TransplantationProgenitor CellsHSCsHSCTReconstitutionControl of hematopoietic stemMyeloidUmbilicalPost-engraftmentPlatelet engraftmentDonor cellsPatient'sImmunodeficientCD34ChimerismMultilineageMarrowInfectionsMiceVivoProgenitorsCellChemotherapyPatientsHumanPhaseRepopulateMyeloablativePostengraftmentCellsRegimenTissue
Transplantation31
- Successful stem cell transplantation for patients with severe combined immunodeficiency (SCID) from matched family donors without conditioning results in engraftment of T lymphocytes. (nih.gov)
- Full hematopoietic engraftment was reported in one case after bone marrow transplantation without conditioning for a SCID patient. (nih.gov)
- Recovery of cytomegalovirus (CMV)-specific immunity after hematopoietic cell transplantation (HCT) is essential in controlling CMV infection. (umn.edu)
- Our aim was to study the influence of acute graft-versus-host disease (a-GVHD) on primary engraftment times after allogeneic transplantation. (unict.it)
- However, this balance is altered during the hematopoietic recovery after Hematopoietic Stem and Progenitor Cell Transplantation . (bvsalud.org)
- Transplantation efficacy can be limited by inadequate Hematopoietic Stem Cells number, poor homing, low level of engraftment, or limited self -renewal. (bvsalud.org)
- Hematopoietic stem cell transplantation (HSCT) involves the intravenous infusion of hematopoietic stem cells in order to reestablish blood cell production in patients whose bone marrow or immune system is damaged or defective. (medscape.com)
- HSCs specifically are defined by their ability for self-renewal and to reconstitute the entire hematopoietic system following transplantation. (stemcell.com)
- It is used both by hematopoietic researchers and clinical labs to assess the potency of transplantation units of cord blood (CB) and hematopoietic cellular therapy products. (stemcell.com)
- Other uses of the CFU assay include studying the effects of stimulatory and inhibitory growth factors, screening novel compounds to predict potential toxicity to the hematopoietic system, and testing the effects of various in vitro manipulations (e.g. cell processing, cryopreservation, gene transduction, and transmission) on cellular products used in hematopoietic cell transplantation. (stemcell.com)
- Kamble RT, Guo S, Ramos CA, Carrum G " Acute gout at engraftment following hematopoietic transplantation. . (bcm.edu)
- Gurkan E, Patah PA, Saliba RM, Ramos CA, Anderson BS, Champlin R, de Lima M, Lichtiger B " Efficacy of prophylactic transfusions using single donor apheresis platelets versus pooled platelet concentrates in AML/MDS patients receiving allogeneic hematopoietic stem cell transplantation. . (bcm.edu)
- Although allogeneic hematopoietic cell transplantation (alloHCT) is the treatment of choice, patient eligibility criteria and optimal conditioning regimen remain under study. (confex.com)
- Therefore, hematopoietic stem cells mobilized into a subject's peripheral blood by the method of the invention is useful as a source of donor cells in bone marrow transplantation for the treatment of a variety of disorders, including cancer, anemia, autoimmunity and immunodeficiency. (justia.com)
- Umbilical cord blood (UCB) is a valuable source of hematopoietic stem cells (HSCs) and potential alternative for bone marrow transplantation for patients who lack human leukocyte antigen (HLA)-matched donors. (wjgnet.com)
- Hematopoietic stem cell transplantation is the only potentially curative therapy, but it is only an option for select patients. (cancernetwork.com)
- This phase I trial assessed the safety, efficacy, and immunologic responses to minor histocompatibility antigens following nonmyeloablative allogeneic hematopoietic cell transplantation as treatment for metastatic renal cell carcinoma. (aacrjournals.org)
- Eight patients received conditioning with fludarabine and low-dose total body irradiation followed by hematopoietic cell transplantation from an HLA-matched sibling donor. (aacrjournals.org)
- Treatment of metastatic renal cell carcinoma with allogeneic hematopoietic cell transplantation after nonmyeloablative conditioning with fludarabine/total body irradiation is feasible and may induce tumor regression or stabilization in some patients. (aacrjournals.org)
- She is currently Research Scientist at Stanford University, where she is developing innovative cell therapy approaches to address neuropathic lysosomal storage diseases (LSDs) using hematopoietic stem cell transplantation (HSCT) and genome editing. (stanford.edu)
- Autologous hematopoietic stem cell transplantation using genome-edited cells can become a definitive therapy for hematological and non-hematological disorders with neurological involvement. (stanford.edu)
- These strains show not only a high rate of human cell engraftment, but also generate well-differentiated multilineage human hematopoietic cells after human hematopoietic stem cell (HSC) transplantation. (nih.gov)
- Hematopoietic stem cell transplantation is also sometimes used for solid tumors (eg, some germ cell tumors) that respond to chemotherapy. (msdmanuals.com)
- Hematopoietic stem cell transplantation may be autologous (using the patient's own cells) or allogeneic (using cells from a donor). (msdmanuals.com)
- Peripheral blood has largely replaced bone marrow as a source of stem cells, especially in autologous hematopoietic stem cell transplantation, because stem cell harvest is easier and neutrophil and platelet counts recover faster. (msdmanuals.com)
- Umbilical cord hematopoietic stem cell transplantation has been restricted mainly to children because there are too few stem cells in umbilical cord blood for an adult. (msdmanuals.com)
- There are no contraindications to autologous hematopoietic stem cell transplantation. (msdmanuals.com)
- Allogeneic hematopoietic stem cell transplantation is limited mainly by lack of histocompatible donors. (msdmanuals.com)
- The technique for umbilical cord hematopoietic stem cell transplantation is still in its infancy, but it is gaining interest. (msdmanuals.com)
- Because cord blood contains immature stem cells, HLA matching appears less crucial than for the other types of hematopoietic stem cell transplantation. (msdmanuals.com)
- The growing need for haematopoietic stem cell transplantation (HSCT) is reflected in the increasing number of transplants performed globally each year. (bvsalud.org)
Progenitor Cells14
- Natural estrogens enhance the engraftment of human hematopoietic stem and progenitor cells in immunodeficient mice. (bvsalud.org)
- Hematopoietic Stem and Progenitor Cells are crucial in the maintenance of lifelong production of all blood cells . (bvsalud.org)
- As recent evidences indicate that estrogens are involved in regulating the hematopoiesis , we sought to examine whether natural estrogens ( estrone or E1, estradiol or E2, estriol or E3 and estetrol or E4) modulate human Hematopoietic Stem and Progenitor Cells . (bvsalud.org)
- We found that both E2 and E4 expand human Hematopoietic Stem and Progenitor Cells . (bvsalud.org)
- Furthermore, we identified that E2 and, more significantly, E4 doubled human hematopoietic engraftment in immunodeficient mice without altering other Hematopoietic Stem and Progenitor Cells properties. (bvsalud.org)
- This application might have particular relevance to ameliorate the hematopoietic recovery after myeloablative conditioning, especially when limiting numbers of Hematopoietic Stem and Progenitor Cells are available. (bvsalud.org)
- Also making up a part of the hematopoietic system are short-term repopulating progenitor cells, which give rise to lineage-specific cell types. (stemcell.com)
- Collectively, these are referred to as hematopoietic stem and progenitor cells (HSPCs). (stemcell.com)
- Improved methods and pharmaceutical compositions are provided herein for mobilizing hematopoietic progenitor cells from bone marrow into peripheral blood, comprising the administration of an effective amount of an inhibitor of GTPases, such as Rac1 and Rac2 alone or in combination. (justia.com)
- Current treatments to manage the problems that result from prolonged bone marrow suppression include the reinfusion of a patient's own previously harvested hematopoietic stem and progenitor cells. (justia.com)
- In such procedures, patients undergo successive treatments with cell mobilization agents to cause mobilization of hematopoietic progenitor cells from the bone marrow to the peripheral circulation for harvesting. (justia.com)
- Small hepatic progenitor cells (SHPCs) derived from these hepatocytes have shown mature metabolic functions as well as engraftment in immunodeficient mice. (warf.org)
- Therapeutic Candidate or Device The therapeutic product candidate is autologous CD34+ hematopoietic stem/progenitor cells (HSPC) transduced with the G1XCGD lentiviral vector. (ca.gov)
- This article contains highlights of "Guidelines for Pre- allogeneic or autologous, depending on the source of venting Opportunistic Infections among Hematopoi- the transplanted hematopoietic progenitor cells. (cdc.gov)
HSCs6
- HSCs make up a very small population of the hematopoietic system, however, these cells are invaluable as they have the potential to give rise to all mature blood and immune cell types and sustain life-long blood production. (stemcell.com)
- These severely immunodeficient animals are required to support human cell engraftment to generate multiple differentiated lineages from human hematopoietic stem cells (HSCs). (genengnews.com)
- It maintains normal hematopoietic function by interacting with hematopoietic stem cells (HSCs) to control their proliferation and differentiation ( Morrison and Scadden, 2014 ). (techscience.com)
- However, human hematopoietic cells developed from HSCs are not always phenotypically and functionally identical to those in humans. (nih.gov)
- Results from this study showed that antibodies targeting the CD117 receptor on FA hematopoietic stem cells (HSCs) enables safe and effective HCT in FA mice when combined with T-cell depleting antibodies. (fanconi.org)
- Engraftment of donor cells in mouse models also showed that when specific immunosuppressant regimens are used donor HSCs have a competitive advantage over FA HSC cells. (fanconi.org)
HSCT1
- Opportunistic infections (OIs) are defined as any in- the infusion of hematopoietic stem cells from a donor fections that occur with increased frequency or severity into a patient who has received chemotherapy, which in HSCT patients. (cdc.gov)
Reconstitution5
- In conclusion, a-GVHD may influence early marrow reconstitution and is a relevant factor for primary myeloid and platelet engraftment. (unict.it)
- Together, our data demonstrate that E4 is well tolerated and enhances human reconstitution in immunodeficient mice , directly by modulating human Hematopoietic Progenitor properties and indirectly by interacting with the bone marrow niche. (bvsalud.org)
- The success of treatment crucially depends on the mobilization of the bone marrow stem cells, the subsequent return of which permits the patient to achieve reconstitution of a functioning hematopoietic system. (justia.com)
- However, the use of UCB is limited by delayed engraftment and poor immune reconstitution, leading to a high rate of infection-related mortality. (wjgnet.com)
- The research interests in my lab are on the development of new approaches to enhance hematopoietic stem cell engraftment and reconstitution following blood and bone marrow transplant. (dukehealth.org)
Control of hematopoietic stem1
- There his work focused on transcriptional control of hematopoietic stem cell maturation and cell fate. (ubc.ca)
Myeloid3
- Source of engraftment determination included preferentially peripheral blood CD3 fraction, then myeloid cell fraction (CD15+), then bone marrow. (umn.edu)
- Criteria for discharge were presence of myeloid engraftment and absence of severe concomitant problems. (nature.com)
- Patients were monitored for donor engraftment of myeloid and lymphoid cells, for clinical response by serial imaging, and for immunologic response by in vitro isolation of donor-derived CD8 + CTLs recognizing recipient minor histocompatibility (H) antigens. (aacrjournals.org)
Umbilical1
- CRL's CD34 + humanized NCG model, HuCD34-NCG, injected with umbilical cord blood-derived CD34 + cells shows robust engraftment of major human immune cells and demonstrates infiltration of T cells into tissues and xenograft tumors. (genengnews.com)
Post-engraftment2
- Pre- and post-engraftment bloodstream infection rates and associated mortality in allogeneic hematopoietic stem cell transplant recipients. (uchicago.edu)
- HuCD34-NCG mice sustain humanization through day 236 post engraftment. (genengnews.com)
Platelet engraftment2
- When factors important for engraftment were studied in a multivariate analysis, postengraftment a-GVHD was a significant factor in delayed neutrophil and platelet engraftment. (unict.it)
- Additionally, the study met key secondary endpoints related to the speed of platelet engraftment, decrease in infections and reduction in hospitalizations, all significant clinical measures in bone marrow transplant. (biospace.com)
Donor cells1
- these mice carry the SIRP-alpha gene similar to humans that allows the engraftment of donor cells. (genengnews.com)
Patient's2
- These therapies decrease a patient's white blood cell counts, suppress bone marrow hematopoietic activity, and increase the patient's risk of infection and/or hemorrhage. (justia.com)
- A key milestone in a patient's recovery, neutrophil engraftment is a measure of how quickly the stem cells a patient receives in a bone marrow transplant are established and begin to make healthy new cells. (biospace.com)
Immunodeficient2
- Finally, the impact of E4 on promoting human hematopoietic engraftment in immunodeficient mice might be mediated through the regulation of mesenchymal stromal cells in the bone marrow niche. (bvsalud.org)
- The inventors have previously shown that such SHPCs express high levels of mature hepatic genes, perform xenobiotic metabolism at rates equal to primary human hepatocytes, and support engraftment in immunodeficient mice (P180325). (warf.org)
CD341
- In this regard, his lab has identified a novel family of hematopoietic cell surface proteins, called the CD34 family, and shown that these are essential for a number of developmentally important processes. (ubc.ca)
Chimerism4
- In contrast to the expected mixed chimerism that usually occurs in the absence of conditioning, we found in our patients 100% donor cell engraftment based on fluorescence in situ hybridization (FISH) and microsatellite techniques. (nih.gov)
- We hypothesize that mixed donor engraftment as measured by chimerism at day 30 in CMV D(+) HCTs and full chimerism in CMV D(−) HCTs will be predictive of CMV reactivation. (umn.edu)
- Chimerism analysis - testing of short tandem repeat regions to assess donor bone marrow engraftment. (ouh.nhs.uk)
- All patients achieved initial mixed hematopoietic chimerism with two patients rejecting their graft and recovering host hematopoiesis. (aacrjournals.org)
Multilineage1
- Multilineage donor engraftment is possible in SCID patients even without conditioning. (nih.gov)
Marrow15
- Primary engraftment and frequency of marrow granulocyte-macrophage colony-forming units and erythroid burstforming units, at day +18, were studied in 126 allogeneic transplants. (unict.it)
- Newer research indicates that other graft-versus-host disease target organs include the immune system (the hematopoietic system , e.g., the bone marrow and the thymus ) itself, and the lungs in the form of immune-mediated pneumonitis . (wikipedia.org)
- NOD-SCID-Gamma (NSG) mice) and measuring the presence of human blood cells in the blood or bone marrow (BM) after extended periods of engraftment, e.g., 20 weeks or longer. (stemcell.com)
- The present invention relates generally to improved methods and pharmaceutical compositions for mobilizing hematopoietic stem and progenitor cell from bone marrow into peripheral blood by administration of at least one inhibitor of a GTPase, such as Rac1 and/or Rac2 GTPase. (justia.com)
- The use of high-dosage chemotherapy or radiotherapy for bone marrow ablation requires subsequent incorporation of hematopoietic stem cells into the patient, in which case prior harvesting of such cells is required. (justia.com)
- The bone marrow stromal cell (BMSC) population, which makes up a large part of the hematopoietic microenvironment, encompasses a variety of adherent cell types. (techscience.com)
- Blood stem cells, also known as hematopoietic stem cells , are found in the bone marrow, where they self-renew as well as differentiate to create all types of blood cells. (medicalxpress.com)
- Extramedullary hematopoiesis (EMH) is likely the result of abnormal trafficking of hematopoietic stem cells (HSC) from the bone marrow to organs such as the spleen, liver, and lung, causing organomegaly and sometimes organ dysfunction. (cancernetwork.com)
- The first area is to define the genes and molecules in the bone marrow stem cells and in the bone marrow micro-environment that regulate hematopoietic stem cell function and number. (dukehealth.org)
- Angiocrine is developing a cell therapy aimed to improve the availability and engraftment of blood stem cell transplants for cancer patients who have had their cancerous bone marrow removed by chemotherapy. (ca.gov)
- Hematopoietic stem cells in mammals are known to reside mostly in the bone marrow, but also transitively passage in small numbers in the blood. (uzh.ch)
- Proof-of-concept studies using human genome-edited hematopoietic stem cells have been hindered by the low efficiency of engraftment of the edited cells in the bone marrow and their modest efficacy in the CNS. (stanford.edu)
- Compared with sub-lethal irradiation, Busulfan conditioning enhanced the engraftment of edited CD34+ cells in the bone marrow, as well the long-term homing and survival of bone-marrow-derived cells in viscera, and in the CNS, resulting in higher transgene expression and biochemical correction in these organs. (stanford.edu)
- The company also highlighted progress with omidubicel , an advanced cell therapy with positive Phase 3 clinical data, as a potentially life-saving treatment option for patients in need of an allogeneic hematopoietic stem cell (bone marrow) transplant, and GDA-201 , a natural killer (NK) cell immunotherapy in Phase 1/2 development for patients with non-Hodgkin lymphoma (NHL). (biospace.com)
- Immature Granulocyte count (IMG) is used in the assessment of various infections or sepsis and its severity 1,2 , inflammatory conditions like Pancreatis 3 , Appendicitis 4 , Acute Respiratory Distress Syndrome (ARDS) 5 , different diseases of bone marrow, transplant engraftment or growth factor therapy or hematopoietic neoplasms. (horiba.com)
Infections2
- We conclude that in spite of rapid engraftment, non-hematological toxicities and infections remain important limitations for further reduction of the length of patient hospitalization in a significant number of patients after PBSCT. (nature.com)
- Hopefully, following the recommendations made in the guidelines will reduce morbidity and mortality from opportunistic infections in hematopoietic stem cell transplant recipients. (cdc.gov)
Mice2
- In this review, we describe the current knowledge of human hematopoietic cells developed in these mice. (nih.gov)
- NOD/LtSz-Rag1nullPfpnull mice: a new model system with increased levels of human peripheral leukocyte and hematopoietic stem-cell engraftment. (umassmed.edu)
Vivo1
- Evaluation of serum-free culture conditions able to support the ex vivo expansion and engraftment of human hematopoietic stem cells in the human-to-sheep xenograft model. (wakehealth.edu)
Progenitors2
- However, E4 was the best tolerated estrogen and promoted cell cycle of human Hematopoietic Progenitors. (bvsalud.org)
- The inventors have developed a novel system for co-differentiation of hepatocytes and hematopoietic progenitors from human embryonic stem cells (ES cells) that mimics a late fetal liver undergoing hematopoiesis. (warf.org)
Cell12
- Our results show that human Hematopoietic Stem and Progenitor Cell subsets express estrogen receptors , and whose signaling is activated by E2 and E4 on these cells . (bvsalud.org)
- Quality assurance of hematopoietic stem cells by macrophages determines stem cell clonality. (bu.edu)
- Distinct Roles for Matrix Metalloproteinases 2 and 9 in Embryonic Hematopoietic Stem Cell Emergence, Migration, and Niche Colonization. (bu.edu)
- Flow cytometry analysis of peripheral blood at 12 and 16 weeks post injection verified engraftment of human cell subpopulations. (genengnews.com)
- The various mature blood cell types are all ultimately derived from a single class of progenitor cell known as hematopoietic stem cells. (justia.com)
- Other cell types derived from hematopoietic stem cells include platelets and erythrocytes. (justia.com)
- In addition, he identified a number of novel hematopoietic stem cell surface proteins and began analyzing their function. (ubc.ca)
- His laboratory has followed two primary interests: 1) the transcription factor networks that regulate fate determination in various cells that make blood, and 2) the cell surface proteins expressed by hematopoietic stem cells that and allow them to communicate with their microenvironment. (ubc.ca)
- Myelofibrosis (MF) is a hematopoietic stem cell malignancy classified as a myeloproliferative neoplasm (MPN). (cancernetwork.com)
- The goal of this grant was to develop a non-genotoxic antibody-based conditioning regimen that can be used to treat people with FA who are undergoing a hematopoietic cell transplant (HCT). (fanconi.org)
- and hematopoietic stem cell safety. (cdc.gov)
- SCID is fatal in the first 2 years of life unless the patient is treated with extremely restrictive isolation, hematopoietic stem cell transplant or therapy that replaces the abnormal gene or gene product. (lu.se)
Chemotherapy1
- This is defined by their ability to repopulate an individual whose hematopoietic system has been destroyed by radiation or chemotherapy. (justia.com)
Patients3
- The cumulative incidence of CMV infection 1-year after transplant was not different in patients with full (54%, n = 276) or mixed (53%, n = 131) donor engraftment. (umn.edu)
- In many cases, successfully mobilization is not effected in the patient and inadequate numbers of hematopoietic stem cells are harvested from these patients using current methods. (justia.com)
- Patients with evidence of maternal engraftment or an elevated response to phytohemagglutinin (PHA) should receive RETHYMIC with immunosuppressive medications (Table 2). (rxlist.com)
Human3
- Polymorphism in Sirpa modulates engraftment of human hematopoietic stem cells. (wikipedia.org)
- They robustly support the engraftment of human cells, and develop functional human immune, systems making them ideal for studying immune-related therapies in a vast array of applications. (genengnews.com)
- Improved engraftment and therapeutic efficacy by human genome-edited hematopoietic stem cells with Busulfan-based myeloablation. (stanford.edu)
Phase1
- During the engraftment phase, which lasts several weeks, the healing process begins with resolution of mucositis and other acquired lesions. (medscape.com)
Repopulate1
- The subject's blood can be processed and used to repopulate the destroyed lymphohematopoietic system of a recipient and may in the future be utilized to repair a variety of non-hematopoietic tissues. (justia.com)
Myeloablative1
- At day +30, full donor engraftment was seen in 82% of myeloablative and 55% of RIC transplants. (umn.edu)
Postengraftment1
- and the postengraftment a-GVHD group are those who were not on corticosteroid treatment at the time of engraftment but needed it after day +19. (unict.it)
Cells5
- Additional markers can be used to distinguish HSPC subsets within the CD34+ population and isolate HSPCs with different engraftment abilities and capacities to expand or generate mature blood cells in culture. (stemcell.com)
- Specifically, methods are disclosed for mobilizing hematopoietic stem cells into a subject's peripheral blood. (justia.com)
- In particular, embodiments of the method involve inhibition of both Rac1 and Rac2 GTPases to increase the numbers of hematopoietic stem cells into a subject's peripheral blood of a subject. (justia.com)
- Leukocytic, hematopoietic cells are important in maintaining the body's defense against disease. (justia.com)
- We also analyse the engraftment of donor stem cells into non-conditioned and conditioned hosts, quantifying the impact of different treatment scenarios. (uzh.ch)
Regimen1
- We have therefore examined whether TCD compromises engraftment using a fludarabine-based conditioning regimen. (qub.ac.uk)
Tissue1
- During the initial development of the HuCD34-NCG mouse, peripheral blood samples were collected at various timepoints, along with periodic tissue sampling, to verify engraftment and sustainability. (genengnews.com)