Engineering strain metabolic engineering. Medical search. Frequent questions

Therefore, yeast strains engineered to produce other products are suboptimal due to their production of ethanol as a major byproduct that significantly diminishes the yield of the desired product. (aiche.org)
Strains have been developed to overcome this central challenge in yeast metabolic engineering by deleting either pyruvate decarboxylases, or alcohol dehydrogenases involved in ethanol formation, which dramatically reduces ethanol formation. (aiche.org)
We have developed a new yeast strain that produces almost no ethanol, has improved fitness when growing on glucose, and overcomes many of the limitations that exist in currently available strains. (aiche.org)
2015. Metabolic engineering of atyrosine-overproducing yeast platform strain evaluated by targetedmetabolomics. (concordia.ca)
Use of Modified Yeast Strains in Distillers Co-Products ∗ Growth in the U.S. ethanol industry has Increased interest in the development of modified yeast strains to improve ethanol production. (slideshare.net)
This has led to increased interest in the use of modified yeast strains in distillers co-products. (slideshare.net)
∗ Industrial use of modified yeast strains is regulated by EPA under its TSCA biotechnology rules. (slideshare.net)
Capitalizing on the existing expertise in reconstruction of carnosic acid in yeast, I applied an interdisciplinary approach that involve metabolic engineering, lipid biochemistry and biophysics to study the role of membrane lipid composition on the CYPs activity and the assembly of the metabolic complex involved in this pathway. (europa.eu)
Here, we engineered the thermotolerant yeast Kluyveromyces marxianus to create a new synthetic biology platform. (asm.org)
However, major genetic engineering limitations have kept this yeast from replacing the commonly used yeast Saccharomyces cerevisiae in industrial applications. (asm.org)
The yeast Saccharomyces cerevisiae has served as the major eukaryotic organism for synthetic biology, but lacks the metabolic potential that could be exploited in many of the more than one thousand yeast species that have been identified to date. (asm.org)
More recently, researchers engineered strains of E. coli and yeast with the capacity to produce taxidene and other isoprenoid compounds [ 4 ][ 6 ]. (openwetware.org)
Still, those engineered strains of E. coli and yeast serve as platform technologies for tractable expression of other newly discovered enzymes. (openwetware.org)

A combination of succinate and ethanol can be fermented to produce butyrate (a precursor to butanol fuel) by utilizing the metabolic pathways present in Clostridium kluyveri. (wikipedia.org)
Presentation at 2016 Fuel Ethanol Workshop, describing U.S. regulatory pathways for approval of use of modified microorganisms in animal feed, and a proposal for making such pathways simpler and more efficient for modified strains of common microorganisms. (slideshare.net)
Here, systems metabolic engineering , which is the key component for biorefinery technology, is utilized to effectively engineer the complex metabolic pathways of microorganisms to enable the efficient production of industrial chemicals. (phys.org)
The authors describe, in depth, the biosynthetic pathways, physical properties and industrial production processes and discuss in detail the genetic and metabolic engineering of microorganisms for biopolymer production. (caister.com)
However, the promoters used in most pathway engineering studies in S. cerevisiae have not been characterized and compared in parallel under multiple conditions that are routinely operated in laboratory and the number of known promoters is rather limited for the construction of large biochemical pathways. (nih.gov)
Metabolic engineering for glycoglycerolipids production in E. coli: tuning phosphatidic acid and UDP-glucose pathways. (iqs.edu)

In this work, a modeling approach was applied to describe the physiological behavior and the metabolic fluxes of a shikimic acid overproducing Escherichia coli strain lacking the major glucose transport system, grown on complex media. (biomedcentral.com)
Both model sets provided information that could be applied to enhance shikimic acid production on an engineered shikimic acid overproducing Escherichia coli strain. (biomedcentral.com)
Coenzyme Q production by metabolic engineered Escherichia coli strains" by Irene Martinez, Nataly Maturana et al. (engconfintl.org)
A newly discovered receptor in a strain of Escherichia coli can be blocked to avert infection, a finding that might aid in developing better therapies to treat bacterial infections resulting in food poisoning, diarrhea or plague. (sciencedaily.com)
Change in cofactor specificity of oxidoreductases by adaptive evolution of an escherichia coli nadph-auxotrophic strain. (mpg.de)

The book series Developments in Microbial Cell Factories: From Design to Commercial Production publishes the latest research findings and technological developments in the area of isolation and characterization of microbial strains, strain development and improvement, optimization bioprocesses and large-scale production of valuable products by prokaryotic and eukaryotic microorganisms such as archaea, bacteria, yeasts, fungi, microalgae, and protozoa. (elsevier.com)
Among microorganisms producing succinic acid, Mannheimia succiniciproducens has been proven to be one of the best strains for succinic acid production. (phys.org)
The genetic manipulation of microorganisms opens up an enormous potential for the biotechnological production of biopolymers with tailored properties suitable for high-value medical application such as tissue engineering and drug delivery. (caister.com)
Synthetic biology is used to harness the metabolic capacity of microorganisms for the biosynthesis of simple and complex compounds now sourced unsustainably from fossil fuels or that are too expensive to make using chemical synthesis at industrial scale. (asm.org)

In its engineered form, E. coli produces the highest yields of isobutanol of any microorganism. (wikipedia.org)
citation needed] Methods such as elementary mode analysis have been used to improve the metabolic efficiency of E. coli so that larger quantities of isobutanol may be produced. (wikipedia.org)
Researchers at UT Southwestern Medical Center are the first to identify the receptor, known as QseC, used by a diarrhea-causing strain of E coli to receive signals from human flora and hormones in the intestine and express virulence genes to initiate infection. (sciencedaily.com)
Design and engineering of E. coli metabolic sensor strains with a wide sensitivity range for glycerate. (mpg.de)
We will additionally discuss recent findings regarding the metabolic challenges associated with producing recombinant silk in bacteria, and we will present our work in engineering a new E. coli strain with significantly improved capacity for synthesizing recombinant silk. (techconnectworld.com)

These imbalances can disrupt precursor availability and energy balances, causing the accumulation of pathway intermediates and unwanted byproducts, reducing strain fitness and product yields [ 16 ]. (biomedcentral.com)
Ethanol pathway engineering. (researchgate.net)
To augment ethanol production by the ET strain, an alternative ethanol production pathway comprised of pyruvate decarboxylase (Pdc) and alcohol dehydrogenase (AdhB) from Zymomonas mobilis (Fig. 4A) was transferred into C. phytofermentans on the replicating pQexpE plasmid (Fig. 4B). (researchgate.net)
In this strain, energy redistribution, and metabolic rerouting through the pentose phosphate pathway (PPP) generated excess reductants while uncoupling growth from hydrogen synthesis. (eol.org)
They carried out systems metabolic engineering to optimize the succinic acid production pathway of the M. succiniciproducens strain by determining the crystal structure of key enzymes important for succinic acid production and performing protein engineering to develop enzymes with better catalytic performance. (phys.org)
As an example of application for these promoters in metabolic engineering, the genes involved in xylan degradation and zeaxanthin biosynthesis were subsequently cloned under the control of promoters with medium to high strength and assembled into a single pathway. (nih.gov)
The corresponding construct was transformed to a S. cerevisiae strain integrated with a D-xylose utilizing pathway. (nih.gov)
However, the microbial metabolic pathway of the fungicide and the genetic systems driving its degradation by strain P3 remain unknown. (nature.com)
Therefore, elucidation of the microbial metabolic pathway of OPP is a prerequisite for the downstream exploitation of strain P3 in any environmental application. (nature.com)
6 identified a gene cluster, hbpCAD , encoding the upper metabolic pathway of OPP which involves the transformation of OPP to 2-hydroxypenta-2,4-dienoateand benzoic acid (BA). (nature.com)
The downstream transformation of BA involved a meta -cleavage pathway, although its genetic organization and function was not revealed and the overall network of genes driving the full metabolic pathway of OPP is still not known. (nature.com)
Regarding second-generation or advanced biofuels, bioprospecting techniques are becoming an increasingly important strategy for biochemical pathway engineering and overall optimization. (openwetware.org)
Corynebacterium glutamicum has recently been engineered to produce biofuel additives 2-methyl-1-butanol and 3-methyl-1-butanol isomers via the Ehrlich pathway to accumulate 2-keto-3-methylvalerate and 2-ketoisocaproate, respectively. (pharmiweb.com)
This workflow maps microarray data onto metabolic pathway diagrams represented as SBML models drawn using Cell Designer. (myexperiment.org)

Plasmid-based synthetic sRNA expression system does not leave any scar on the chromosome, and can be easily transferred to many other host strains to be examined. (sciencedaily.com)
Thus, the construction of libraries and examination of different host strains are much easier than the conventional hard-coded gene manipulation systems. (sciencedaily.com)
Our objective is to produce host strains with enhanced resistance to a broad range of chemical products & so provide highly-productive chassis for commercial synthetic biology. (ukri.org)

They are suited for isobutanol biosynthesis when genetically engineered to produce isobutanol and its corresponding aldehydes. (wikipedia.org)

Gene knock-out experiments are often performed to delete those metabolic fluxes that will consequently result in the increase of the desired product formation. (sciencedaily.com)
In addition, we will discuss how reaction atom mapping information in MetRxn can be used to perform metabolic flux elucidation at a genome-scale revealing how the assumptions implied by core metabolic models propagate in the inference of internal metabolic fluxes. (ucsb.edu)

Using a combination of functional genomics, metabolic engineering and synthetic biology techniques, we study microbes normally found in the environment in order to better understand their metabolism and physiology with the purpose of eventually engineering them as alternatives to producing, cleaner, better, cheaper or even novel products. (concordia.ca)
A Korean research team led by Distinguished Professor Sang Yup Lee at the Department of Chemical and Biomolecular Engineering from the Korea Advanced Institute of Science and Technology (KAIST), a science and engineering university in Korea, reported the development of a strategy for efficiently developing microbial cell factories by employing synthetic small RNAs (sRNAs). (sciencedaily.com)
This strategy of employing synthetic sRNAs in metabolic engineering has been receiving great interest worldwide as it allows easy, rapid, high-throughput, tunable, and un-doable knock-down of multiple genes in multiple strains at the same time. (sciencedaily.com)
Now, a paper published online on August 8 as a journal cover paper (September issue) in Nature Protocols , describes the detailed strategy and protocol for employing synthetic sRNAs for metabolic engineering. (sciencedaily.com)
This approach is becoming more feasible thanks to advances in metabolic strain engineering, synthetic biology, and machine learning. (sjsu.edu)
His research focuses on applying and extending the approaches of synthetic biology, systems biology, and protein engineering. (sjsu.edu)
These yeasts remain difficult to use, however, as there are few synthetic biology tools to access their underlying metabolic networks and physiology ( 1 ). (asm.org)
Ginkgo's synthetic biology platform, enables you to develop functional proteins, enzyme biocatalysts, small molecule ingredients, and microbial strains for a variety of applications in the nutrition and wellness markets. (ginkgobioworks.com)
We will combine knowledge of these systems to develop our DeTox strain platform by strain engineering, using synthetic biology standards. (ukri.org)

This is a big problem in developing a high performance microbial cell factory because it is required to find the best platform strain among many different strains. (sciencedaily.com)
For this experiment , researchers created different strains of the microbe that produced more of certain enzymes to see which ones improved isoprenoid production. (glbrc.org)

Several mathematical frameworks have been developed to understand the physiological and metabolic state of production strains and to identify genetic modification targets for improved bioproduct formation. (biomedcentral.com)
Although classic metabolic engineering (ME) allows flux redirection in a biochemical network into valuable compounds by genetic manipulation, it often induces significant flux imbalances to the CCM that may cause undesirable outcomes. (biomedcentral.com)
Therefore, there is an increasing interest into a more global and detailed understanding of the metabolic and regulatory network changes imposed by different genetic modifications or process conditions in various production systems. (biomedcentral.com)
citation needed] Obtaining higher yields of butanol involves manipulation of the metabolic networks using metabolic engineering and genetic engineering. (wikipedia.org)
FDA Genetic Engineering Policy Comments questioned whether a substance (including microbes) that is GRAS could lose its GRAS status solely because it was produced or modified by new biotechnology. (slideshare.net)
He earned his B.S. in Chemical Engineering from The University of Maryland, College Park in 2002, Ph.D. in Chemical Engineering from the Massachusetts Institute of Technology in 2006, and was a postdoctoral research associate at the Whitehead Institute for Biomedical Research from 2006-2008, and at Shire Human Genetic Therapies from 2007-2008. (sjsu.edu)
In between these two levels is the proteome that performs the biochemical transformations according to the genetic template, though it is this middle step in the process that cannot yet be robustly and efficiently incorporated into models of metabolic systems. (nature.com)
Study combined genetic engineering and quantitative analyses to identify enzymes that enhance the conversion of sugar to building blocks of advanced biofuels and other products. (glbrc.org)
In order to understand the metabolic bottlenecks that limit isoprenoid production, scientists with the Great Lakes Bioenergy Research Center combined genetic engineering and quantitative analyses. (glbrc.org)
Here, we describe genetic tools for genome editing and breeding K. marxianus strains, which we use to create a new thermotolerant strain with promising fatty acid production. (asm.org)
The genetic engineering approach hasn't been applied in industrial A. terreus strains to increase itaconic acid production. (biomedcentral.com)
Itaconic acid production could be improved through genetic engineering of the industrially used A. terreus strain. (biomedcentral.com)
Our results will provide a guide for further enhancement of the itaconic acid production level through genetic engineering in future. (biomedcentral.com)

Using metabolic engineering techniques, researchers created transgenic Arabidopsis thaliana capable of producing taxidene, the first committed step in paclitaxel biosynthesis [ 5 ]. (openwetware.org)

Its outcomes aimed to provide insides for engineering advanced cell factories that will enable low-cost, scalable and less time-consuming processes. (europa.eu)
Books in this series cover recent advances and future trends in microbial cell factories and provide an overview from design and improvement of microbial strains to commercial production of valuable products and allowing us to move towards a bio-based economy. (elsevier.com)

As a result, 134 g per liter of succinic acid was produced from the fermentation of an engineered strain using glucose, glycerol, and carbon dioxide. (phys.org)

The research described here also help address basic scientific questions on the molecular regulation and biochemical understanding of the role of membrane composition on functionality of membrane-associated proteins of plant origin that may be involved in formation of yet questionable metabolic complexes or metabolons. (europa.eu)
They systematically engineered strains of Zymomonas to produce more of certain enzymes, or proteins that speed up the metabolic process. (glbrc.org)

This optimized strain can be engineered to produce different fuels or chemicals of interest from glucose, with significantly enhanced yields. (aiche.org)
One of promising applications is high-throughput screening of the target genes to be manipulated and multiple strains simultaneously to enhance the production of chemicals and materials of interest. (sciencedaily.com)
This strategy of integrating systems metabolic engineering with enzyme engineering will be useful for the production of industrially competitive bio-based chemicals. (phys.org)
It can also be engineered to produce isoprenoids, a group of chemicals used to make biofuels, medicines, flavorings, and other valuable products. (glbrc.org)
The bacterium P. putida is being engineered for the conversion of lignin-derived monomers into key chemicals, such as bioplastic or Nylon precursors. (lu.se)

Such simultaneous optimization of gene targets and strains has been one of the big challenges in metabolic engineering. (sciencedaily.com)
Finally, we will present computational tools for strain optimization leading to the microbial overproduction of target biomolecules using both stoichiometric and large-scale kinetic models. (ucsb.edu)
I have been working in different projects in biotechnology/chemical/food engineering in both upstream and downstream processes aiming for process optimization. (lu.se)

Production of these compounds in engineered microbes can provide a sustainable solution. (europa.eu)
Using engineered microbes, protein-based plastics can be made from renewable resources. (techconnectworld.com)

Furthermore, the gene knock-out experiments performed in one strain cannot be transferred to another organism and thus the whole experimental process has to be repeated. (sciencedaily.com)
By modifying the activity of a single metabolic gene, we engineered strains of Magnaporthe oryzae with different nutrient acquisition and growth rates. (exeter.ac.uk)
Echinocandin-resistant clinical isolates of Candida albicans have been reported, and key-hot spot mutations in the FKS1 gene, which encodes a major glucan synthase subunit, have been identified in these (caspofungin-resistant [CAS-R]) strains. (who.int)

Protein engineering of key enzymes corresponding to succinic acid production. (phys.org)

They isolated a Pseudomonas azelaica strain HPB1 which was able to degrade OPP through the production of 2,3-dihydroxybiphenyl. (nature.com)

Expression of pQexpE increased cellulolysis by ϳ 30% in both the WT and ET strains (Fig. 5A) and boosted ethanol production by 70% relative to the ET strain ( P Ͻ 0.01), thereby restoring ethanol yields to WT levels (Fig. 5B). (researchgate.net)

This will enable formation of multi-molecular complexes (metabolons) responsible for efficient metabolic channeling and coordinated processing of substrates and intermediates. (europa.eu)
By measuring changes in the level of metabolic intermediates, or molecules that can be converted to isoprenoids, researchers were able to identify the primary enzyme limiting carbon metabolism as well as secondary bottlenecks that occurred when levels of the first enzyme were increased, or overexpressed. (glbrc.org)

However, if these enzymatic activities are completely eliminated, the resulting strains are unable to grow on glucose, a preferred industrial feedstock. (aiche.org)
Restoration of growth of these strains on glucose can be achieved through directed evolution, but the resulting strains have impaired fitness and are difficult to work with. (aiche.org)
In the present work, the production of CoQ was assessed in the mentioned strains at several culture conditions including the use of different carbon sources (glucose, glycerol and succinate) and different culture strategies (batch and continuous) in a Lab-Scale Bioreactor. (engconfintl.org)
[1] One of the strains, also known as Tma200, produced 5.77 mol H 2 / mol glucose which is the highest yield so far reported in a fermentative bacterium. (eol.org)

1 Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA. (nih.gov)

Metabolic engineering to reduce byproducts like glycerol. (slideshare.net)

This can be of benefit to researchers who want to explore new metabolic engineering strategies for use of lignin. (lu.se)

In metabolic engineering, it is important to find the genes that need to be amplified and attenuated in order to increase the product formation rate while minimizing the production of undesirable byproducts. (sciencedaily.com)

The editor and the authors have produced an excellent up-to date compendium which is extremely useful for all students of biotechnology, engineering and scientists in the biotechnological and microbiological branches and is recommended for all biotechnological and microbial laboratories and enterprises in this field. (caister.com)

2013. Novel approach toengineer strains for simultaneous sugar utilization. (concordia.ca)

By breeding two of these mating-type engineered K. marxianus strains, we combined three complex traits-thermotolerance, lipid production, and facile transformation with exogenous DNA-into a single host. (asm.org)
The ability to cross K. marxianus strains with relative ease, together with CRISPR-Cas9 genome editing, should enable engineering of K. marxianus isolates with promising lipid production at temperatures far exceeding those of other fungi under development for industrial applications. (asm.org)
Chloroplast engineering of the green microalgae Chlamydomonas reinhardtii for the production of HAA, the lipid moiety of rhamnolipid biosurfactants. (iqs.edu)

In a previous work, we constructed strains unable to produce demethylmenaquinone (DMK) and menaquinone (MK), compounds that compete for both chorismate, precursor of the benzoquinone ring, and the isoprenoid chain. (engconfintl.org)
We validated the algorithm by showing that it can find novel strategies that are growth-coupled in silico for a compound that has not been coupled to growth previously, as well as reproduce known growth-coupled strain designs for two different target compounds. (dtu.dk)
The RetroPath2.0 workflow build a reaction network from a set of source compounds to a set of sink compounds.When applied in a retrosynthetic fashion, the source is composed of the target compounds and the sink is composed of the available reactants (for instance in the context of metabolic engineering the sink is the set of native metabolites of a chassis strain). (myexperiment.org)

Journal of Biological Engineering. (concordia.ca)
He was elected as a Fellow to the American Institute for Medical and Biological Engineering in 2018 and to the National Academy of Inventors in 2019. (sjsu.edu)

We will describe how this resource can impact genome-scale metabolic model reconstruction by providing curated reaction and metabolite content. (ucsb.edu)
In metabolic engineering, strains are modified and controlled at the genome level through the transcriptome, and the effects are observed at the fluxome level, which accounts for the range of metabolic reactions in an organism. (nature.com)
Classically, model-based strain design has relied on tools that use the DNA sequence of an organism and homology with well-studied organisms to infer a network of metabolic reactions that happen inside a cell of that organism, which is called a genome-scale model (GEM). (nature.com)

Metabolic engineering in this bacterium led to development of strains of T. maritima that surpassed the Thauer limit of hydrogen production. (eol.org)

Applications of protein engineering to members of the old yellow enzyme family. (microbiologyresearch.org)

A strain of Clostridium cellulolyticum, a native cellulose-degrading microbe, affords isobutanol directly from cellulose. (wikipedia.org)

Classic metabolic engineering strategies often induce significant flux imbalances to microbial metabolism, causing undesirable outcomes such as suboptimal conversion of substrates to products. (biomedcentral.com)

A strain of Clostridium can convert nearly any form of cellulose into butanol even in the presence of oxygen. (wikipedia.org)

Metabolic modeling, which helps making sense of the metabolism in a biological network, is an important tool for engineering biocatalysts, with applications in biofuels, drug design, microbial community analysis, and personalized medicine. (nature.com)

Comparative aspects based on recent genomic data of various Xanthomonas strains are also covered. (caister.com)

2016.Engineering of a nepetalactol-producing platform strain of Saccharomycescerevisiae for the production of seco-iridoids. (concordia.ca)

However, S. cerevisiae has proven difficult to engineer to expand the carbon sources it can utilize, the products it can make, and the harsh conditions it can tolerate in industrial applications. (asm.org)

Joining AIChE gives you access to an amazing network of top professionals in chemical engineering and related fields. (aiche.org)
Dr. Hal Alper is the Kenneth A. Kobe Professor in Chemical Engineering and Executive Director of the Center for Biomedical Research Support at The University of Texas at Austin. (sjsu.edu)
Wison Engineering Services Co. Ltd., together with its subsidiaries, announced that Wison Engineering Ltd., an indirect non-wholly owned subsidiary of the Group, has been awarded a contract for the engineering of Erdos Jinchengtai coal-to-methanol Project (Phase II) for Erdos Jinchengtai Chemical Co., Ltd. Wison Engineering will partner with Erdos Jinchengtai. (greencarcongress.com)

A research team led by Distinguished Professor Sang-Yup Lee reported the production of a microbial strain capable of the massive production of succinic acid with the highest production efficiency to date. (phys.org)
The research team has developed a bio-based succinic acid production technology using the M. succiniciproducens strain isolated from the rumen of Korean cows for over 20 years and succeeded in developing a strain capable of producing succinic acid with the highest production efficiency. (phys.org)
Metabolic efficiency reshapes the seminal relationship between pathogen growth rate and virulence. (exeter.ac.uk)
We conducted in planta infection studies and uncovered an unexpected non-monotonic relationship between growth rate and virulence that is jointly shaped by how growth rate and metabolic efficiency interact. (exeter.ac.uk)

Transglycosylation activity of engineered Bifidobacterium lacto- N -biosidase mutants at donor subsites for lacto- N -tetraose synthesis. (iqs.edu)
We first sought to assess biofilm formation and structure in these fks1 mutants and found that the biofilm mass and metabolic activities were reduced in most of the fks1 mutants as compared with reference strain SC5314. (who.int)

The combination of dynamic metabolic modeling and experimental parameter response surfaces was a successful approach to understand and predict the behavior of a shikimic acid producing strain under variable substrate concentrations. (biomedcentral.com)

Anatomy10

Organisms29

Chemicals and Drugs84

Analytical, Diagnostic and Therapeutic Techniques and Equipment39

Psychiatry and Psychology1

Phenomena and Processes67

Disciplines and Occupations13

Technology, Industry, Agriculture28

Information Science7

Health Care6

Yeast13

Pathways6

Escherichia5

Microorganisms4

Coli5

Pathway14

Host strains3

Genetically1

Fluxes2

Synthetic9

Different strains2

Genetic13

Biosynthesis1

Cell Factories2

Fermentation1

Proteins2

Chemicals5

Optimization3

Microbes2

Gene3

Enzymes1

Pseudomonas1

Ethanol production1

Intermediates2

Glucose4

Department of Chemical and Biomolecular Engineering1

Glycerol1

Researchers1

Genes1

Biotechnological1

Utilization1

Lipid3

Compounds3

Biological Engineering2

Genome3

Bacterium1

Protein1

Microbe1

Flux1

Clostridium1

Biocatalysts1

Genomic1

Platform strain1

Utilize1

Chemical3

Efficiency4

Mutants2

Substrate1