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Focal AdhesionsStress FibersFocal Adhesion Protein-Tyrosine KinasesFocal Adhesion Kinase 1Cell AdhesionPaxillinCell Adhesion MoleculesVinculinActinsCytoskeletonCell MovementFocal Adhesion Kinase 2ZyxinProtein-Tyrosine KinasesrhoA GTP-Binding ProteinCytoskeletal ProteinsIntegrinsActininTissue AdhesionsNerve FibersPhosphorylationrho GTP-Binding ProteinsCells, CulturedCrk-Associated Substrate ProteinSignal TransductionIntercellular Adhesion Molecule-1rho-Associated KinasesFibronectinsActin CytoskeletonTalinFibroblastsPhosphoproteinsAntigens, CD29Muscle Fibers, SkeletalMicrofilament ProteinsExtracellular MatrixVascular Cell Adhesion Molecule-1Microscopy, FluorescenceTyrosineDietary FiberCell LineCell ShapePseudopodia3T3 Cellssrc-Family KinasesStress, MechanicalMyosin Type IIMyosin Light ChainsBacterial AdhesionRetinoblastoma-Like Protein p130Protein BindingFluorescent Antibody TechniqueEnzyme ActivationEndothelium, VascularLIM Domain ProteinsTransfectionMechanotransduction, Cellularrac1 GTP-Binding ProteinSyndecan-4Cell SizeEndothelial CellsCytochalasin Drac GTP-Binding ProteinsMolecular Sequence DataMineral FibersGTPase-Activating ProteinsCell Surface ExtensionsCadherinscdc42 GTP-Binding ProteinAmino Acid SequencePhosphotyrosineCell Line, TumorRecombinant Fusion ProteinsProtein Structure, TertiaryNIH 3T3 CellsModels, BiologicalLysophospholipidsSwiss 3T3 CellsrhoB GTP-Binding ProteinProto-Oncogene Proteins pp60(c-src)Integrin alpha5beta1Muscle Fibers, Fast-TwitchMuscle Fibers, Slow-TwitchCell-Matrix JunctionsIntracellular Signaling Peptides and ProteinsBlotting, WesternTime FactorsProtein-Serine-Threonine KinasesAmidesBotulinum ToxinsActomyosinCotton FiberContractile ProteinsEpithelial CellsRNA, Small InterferingAdaptor Proteins, Signal TransducingGuanine Nucleotide Exchange FactorsMicroscopy, ConfocalReceptors, VitronectinNeural Cell Adhesion Molecules
Cultured endothelial cellsUmbilical vein endothelial cellsProteinsAortic endothelial cellsVascular endothelial cellsKinaseCellsCytoskeletonDisassemblyPathwayPhosphorylationExtracellular matrixPlatelet adhesionDynamicsTransendothelial migrationPaxillinIntracellularMechanotransductionMigrationVEGFCascadesFormationElasticSubstrateCollagenNanoBehaviourSurfaceFunctionalEffectsRoleDiseases
Cultured endothelial cells1
Umbilical vein endothelial cells2
Proteins6
  • 8] P. Girard and R. M. Nerem, "Shear Stress Modulates Endothelial Cell Morphology and F-Actin Organization Through the Regulation of Focal Adhesion-Associated Proteins," J. Cell. (fujipress.jp)
  • Release of this auto-inhibitory interaction has been shown to occur within focal adhesions-but not in the cytoplasm-and therefore is thought to require interaction with focal adhesion proteins, potentially as a result of mechanical forces transmitted through the focal adhesion. (wikipedia.org)
  • Other components include signalling molecules such as tyrosine-kinases, GTPases and effectors proteins as in focal adhesion. (u-bordeaux.fr)
  • As demonstrated on LF fibroblasts, the resistance to trypsin was similar in serum-supplemented and serum-free media, i.e., medium without cell adhesion-mediating proteins. (nature.com)
  • Based on all the results, the increased resistance to trypsinization of C2C12, LF, HaCaT, and VSMC cells on amine PPs can be explained most probably by a non-specific cell adhesion such as electrostatic interaction between the cells and amine groups on the material surface, rather than by the receptor-mediated adhesion through serum-derived proteins adsorbed on the PPs. (nature.com)
  • The levels of E-selectin, vascular endothelial cell adhesion molecule-1 (VCAM-1), and intercellular adhesion molecule-1 (ICAM-1) proteins in the nasal mucosal tissue were significantly downregulated in the BYTQ-H group compared to the AR group. (bvsalud.org)
Aortic endothelial cells3
Vascular endothelial cells3
  • E-selectin, which is highly expressed in vascular endothelial cells near tumor and get involved in the all tumor growth steps: occurrence, proliferation and metastasis, is considered as a promise targeted protein for antitumor drug discovery. (bvsalud.org)
  • Rat vascular endothelial cells (RVECs) were patterned on collagen-coated nitrocellulose paper to create a tissue chip. (biomedcentral.com)
  • However, some tissues are exposed to both types of mechanical stimuli, e.g. vascular endothelial cells experience both shear flow and compression [ 16 ]. (biomedcentral.com)
Kinase7
  • PTK2 protein tyrosine kinase 2 (PTK2), also known as focal adhesion kinase (FAK), is a protein that, in humans, is encoded by the PTK2 gene. (wikipedia.org)
  • PTK2 is a focal adhesion-associated protein kinase involved in cellular adhesion (how cells stick to each other and their surroundings) and spreading processes (how cells move around). (wikipedia.org)
  • The PTK2 gene encodes a cytosolic protein tyrosine kinase that is found concentrated in the focal adhesions that form among cells attaching to extracellular matrix constituents. (wikipedia.org)
  • Focal adhesion kinase has four defined regions, or tertiary structure domains. (wikipedia.org)
  • Caspase-mediated cleavage of focal adhesion kinase disassembly and pp125FAK of focal adhesions in individual endothelial cell apoptosis. (opioid-receptors.com)
  • Houle, F., Poirier, A., Dumaresq, J. and Huot, J. (2007) DAP Kinase Mediates the Phosphorylation of Tropomyosin-1 Downstream of the ERK Pathway, Which Regulates the Formation of Stress Fibers in Response to Oxidative Stress. (scirp.org)
  • The interplay between mechanical strain and inflammation in pulmonary fibrosis remain incompletely understood.APPROACH: In this study, we used conditional KO mice to block mechanotransduction by knocking out FAK (Focal Adhesion Kinase) expression in fibroblasts, followed by induction of pulmonary fibrosis using bleomycin. (stanford.edu)
Cells44
  • Cell-cell and cell-matrix adhesions are known to play key roles in the recruitment and activation of immune T-cells. (weizmann.ac.il)
  • Prominent among the molecular adhesion components are integrins (β1 and β2), that mediate the interactions of a variety of immune cells to extracellular matrices, and to other immune cells, respectively. (weizmann.ac.il)
  • Endothelial cells (ECs) adapt to mechanical environments such as cyclic stretching by altering their morphology and cytoskeletal structure. (fujipress.jp)
  • To understand the contribution of strain in contact area between focal adhesions (FAs) and the substrate to morphological and cytoskeletal changes in cells, we applied cyclic stretching to ECs using a microsubstrate with arrays of micropillars on which cells were selectively stretched between FAs but FA-substrate contact area were hardly stretched. (fujipress.jp)
  • 3] T. Takemasa, T. Yamaguchi, Y. Yamamoto, K. Sugimoto, and K. Yamashita, "Oblique Alignment of Stress Fibers in Cells Reduces the Mechanical Stress in Cyclically Deforming Fields," Eur. (fujipress.jp)
  • 9] Y. Shikata, A. Rios, K. Kawkitinarong, N. DePaola, J. G. N. Garcia, and K. G. Birukov, "Differential Effects of Shear Stress and Cyclic Stretch on Focal Adhesion Remodeling, Site-Specific FAK Phosphorylation, and Small GTPases in Human Lung Endothelial Cells," Exp. (fujipress.jp)
  • FAK is a protein of 125 kD recruited as a participant in focal adhesion dynamics between cells, and has a role in motility and cell survival. (wikipedia.org)
  • During early apoptotic signaling in human endothelial cells, FAK is cleaved by caspase 3 at Asp-772, generating two FAK fragments of approximately 90 and 130 kDa in length. (wikipedia.org)
  • Transforming growth factor-ß plays an important role in the development and maintenance of homeostasis of the vascular systems by regulating functions of endothelial cells and smooth muscle cells. (u-bordeaux.fr)
  • In vitro work aims at a full characterization of the molecular composition of podosomes and elucidation of the molecular mechanisms involved in their assembly and disassembly in both microvascular and macrovascular endothelial cells. (u-bordeaux.fr)
  • Our aim is to understand some of the mechanisms by which endothelial cells contribute to the pathophysiology of vascular diseases. (u-bordeaux.fr)
  • We are studying how environmental cues impact on endothelial cells and translate into functional alterations focusing on changes in ECM composition/rigidity and cytokine contexts. (u-bordeaux.fr)
  • TGFß plays a key role in cancer, fibrosis and inflammatory processes and endothelial cells represent a major target of its action. (u-bordeaux.fr)
  • Podosomes are found in a restricted number of cell types (macrophages, immature dendritic cells and osteoclasts) where they seem to be involved in adhesion and invasion. (u-bordeaux.fr)
  • In untreated cells, vinculin is localised at the tips of stress fibers. (u-bordeaux.fr)
  • We have undertaken an extensive characterization of podosomes in different types of endothelial cells. (u-bordeaux.fr)
  • These analyses have brought to light novel components not described previously, which could be involved in specific functions of endothelial cells. (u-bordeaux.fr)
  • Morphologically accurate reproduction of the behavior of endothelial cells is a key to understanding their mechanical behavior in cyclically inflated arteries and to quantitatively correlating this with cellular responses. (springeropen.com)
  • Arterial endothelial cells are exposed to periodic blood flow and undergo associated cyclic deformation. (springeropen.com)
  • For the three-dimensional (3D) modeling and stress analysis of cells subject to fluid shear stress, Ohashi et al. (springeropen.com)
  • Endothelial cells, which form the inner lining of microvessels, create this semipermeable barrier via a combination of cellular junctions, their glycocalyx, and the basement membrane that consists of vessel-specific extracellular matrix (ECM) [ 2 ]. (biomedcentral.com)
  • Embedded within the basement membrane and in direct contact with endothelial cells are pericytes, which are crucial for microvessel maturation, maintenance, as well as proper function. (biomedcentral.com)
  • The microvascular system and especially endothelial cells play a major role in inflammation, act as an endocrine organ and tightly regulate blood coagulation [ 1 ]. (biomedcentral.com)
  • We used brain endothelial cells and mouse models of acid sphingomyelinase-deficient Niemann Pick disease (NPD), and postmortem LSD patients' brains, all compared to respective controls. (ibecbarcelona.eu)
  • NC transcytosis across brain endothelial cells and brain distribution in mice were affected, yet through different mechanisms. (ibecbarcelona.eu)
  • Moreover, cells cultured on a plate showed the active form of the YAP protein and the formation of stress fibers and focal adhesions. (ibecbarcelona.eu)
  • Complimentary to this, fibroblasts exposed to fluid shear stress structurally rearranged pre-coated surface FN, but unstimulated and stretched cells did not. (nature.com)
  • Our previously-obtained impressive results of highly increased C2C12 mouse myoblast adhesion to amine plasma polymers (PPs) motivated current detailed studies of cell resistance to trypsinization, cell proliferation, motility, and the rate of attachment carried out for fibroblasts (LF), keratinocytes (HaCaT), rat vascular smooth muscle cells (VSMC), and endothelial cells (HUVEC, HSVEC, and CPAE) on three different amine PPs. (nature.com)
  • We demonstrated the striking difference in the resistance to trypsin treatment between endothelial and non-endothelial cells. (nature.com)
  • The increased cell adhesion was also confirmed for LF cells by an independent technique, single-cell force spectroscopy. (nature.com)
  • More importantly, the in vivo experiment demonstrated that the conjugate would accumulate of the PODO payload in tumor through targeting endothelial cells in the tumor microenvironment, which resulted in the much improved in vivo inhibition of tumor growth, intratumoral microvessel density, and decreased systemic toxicity of this nanoparticle over the free PODO. (bvsalud.org)
  • Adhesion assays were performed at various periods of time on cells grown on plastic. (scirp.org)
  • Results: We found that cells expressing the phosphomimetic form of Tm1 S283E/Tm1 are characterized by an increased adhesion to the substratum. (scirp.org)
  • Simoneau, B., Houle, F. and Huot, J. (2012) Regulation of Endothelial Permeability and Transendothelial Migration of Cancer Cells by Tropomyosin-1 Phosphorylation. (scirp.org)
  • OBJECTIVES: To investigate the ESG components and their organization on bEnd3 (mouse brain microvascular endothelial cells) monolayer. (semcs.net)
  • The influence of the luminal surface morphology on platelet adhesion and the attachment of endothelial cells was investigated. (biomedcentral.com)
  • In physiological conditions, the lumen of the blood vessel is covered with endothelial cells (ECs), which actively counteract the processes of platelet aggregation and blood coagulation through the synthesis and secretion of various bioactive substances e.g.: nitric oxide, heparan sulphate, prostacyclin [ 6 ]. (biomedcentral.com)
  • In addition, intact endothelial monolayer inhibits the proliferation of smooth muscle cells (SMCs), limiting the risk of intimal hyperplasia. (biomedcentral.com)
  • Another approach, in situ endothelialization, is based on colonization with ECs in the patient's body, which is possible through transanastomotic growth, transmural infiltration, and endothelialization with endothelial progenitor cells circulating in bloodstream [ 7 ]. (biomedcentral.com)
  • The ROCK signaling pathway promotes cell contractility and adhesion of fibroblast cells (e.g. (reviewofoptometry.com)
  • 9,10 It may be that RKI Y-27632 increases outflow facility by relaxing the cytoskeleton and disrupting the connectivity between inner wall endothelial cells and between JCT cells, which in turn may decrease resistance to aqueous by redistributing outflow patterns through looser regions in the JCT and the inner wall. (reviewofoptometry.com)
  • 1998. Activation of alveolar macrophages and peripheral red blood cells in rats exposed to fibers/particles. (cdc.gov)
  • This was also reflected in the expression of stable focal adhesions on PR and AA, thus enabling the formation of intact monolayers of cells on these varieties unlike on BM. (arvojournals.org)
  • Tendon cells synthesize the tendon's extracellular matrix , which abounds with densely-packed collagen fibers. (handwiki.org)
Cytoskeleton3
  • FAK is typically located at structures known as focal adhesions, which are multi-protein structures that link the extracellular matrix (ECM) to the cytoplasmic cytoskeleton. (wikipedia.org)
  • We focus our analysis on endothelial cell's cytoskeleton remodeling and differentiation in response to TGFß and accumulation of pathological matrix. (u-bordeaux.fr)
  • Directed cell migration is a complex process that involves front-rear polarization, characterized by cell adhesion and cytoskeleton-based protrusion, retraction, and contraction of either a single cell or a cell collective. (ibecbarcelona.eu)
Disassembly1
  • Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. (cusabio.com)
Pathway4
  • Proteomics analysis showed that the cell adhesion factors and focal adhesion pathway might be potential mechanism of BYTQ against AR. (bvsalud.org)
  • Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. (cusabio.com)
  • Inhibition of the ROCK pathway not only has great promise for treating glaucoma, but also has therapeutic potential for cardiovascular and pulmonary diseases, prostate cancer, neurological disorders and corneal endothelial wound healing. (reviewofoptometry.com)
  • In the eye, inhibiting the ROCK pathway with RKIs is thought to lower IOP by inducing cellular relaxation and disrupting focal adhesions in the TM and the inner wall endothelial lining of Schlemm's canal. (reviewofoptometry.com)
Phosphorylation3
  • Integrin receptors are heterodimeric transmembrane glycoproteins that cluster upon ECM engagement, leading to FAK phosphorylation and recruitment to focal adhesions. (wikipedia.org)
  • Background: Tropomyosin 1 alpha chain (Tm1) is an actin-binding protein that regulates the endothelial cell response to oxidative stress following its phosphorylation at Serine 283 (S283). (scirp.org)
  • Conclusion: Phosphorylation of Tm1 at Ser283 contributes to its anti-tumor properties, and this effect results mainly from an increase in cell adhesion associated with a decrease in their migratory and invasive potentials. (scirp.org)
Extracellular matrix2
Platelet adhesion1
  • Implantation of synthetic grafts without this endothelium barrier may lead to surface protein adsorption followed by platelet adhesion, activation, and aggregation. (biomedcentral.com)
Dynamics2
  • 9. Rui Y-N, Xu Z, Fang X, Menezes MR, Balzeau J, Niu A, Hagan JP, Kim DH (2017) The Intracranial Aneurysm Gene THSD1 Connects Endosome Dynamics to Nascent Focal Adhesion Assembly. (yoproteins.eu)
  • In keeping with the rapidly developing fields of mechanobiology and regenerative medicine, Biorheology aims to include studies of the rheological aspects of these fields by focusing on the dynamics of mechanical stress formation and the response of biological materials at the molecular and cellular level resulting from fluid-solid interactions. (semcs.net)
Transendothelial migration1
  • In vitro migration assays, transendothelial migration assays (iTEM), and cell adhesion assays were used to investigate the effects of SPON2 on monocyte/macrophage migration. (biomedcentral.com)
Paxillin1
Intracellular2
  • 6] H. Yamada, T. Takemasa, and T. Yamaguchi, "Theoretical Study of Intracellular Stress Fiber Orientation Under Cyclic Deformation," J. Biomech. (fujipress.jp)
  • 7] S. K. Sastery and K. Burridge, "Focal Adhesions: A Nexus for Intracellular Signaling and Cytoskeletal Dyamics," Exp. (fujipress.jp)
Mechanotransduction1
  • 1] P. F. Davis, "Flow-Medicated Endothelial Mechanotransduction," Physiol. (fujipress.jp)
Migration1
  • The increased resistance observed for the non-endothelial cell types was accompanied by an increased rate of cellular attachment, even though spontaneous migration was comparable to the control, i.e., to the standard cultivation surface. (nature.com)
VEGF2
  • For example, RhoA is one of the downstream signaling cascades activated by vascular endothelial growth factor ( VEGF ). (cloudfront.net)
  • ROCK1 acts as a negative regulator of VEGF endothelial cell activation and angiogenesis. (cloudfront.net)
Cascades1
  • Our studies aim at a better understanding of the signaling cascades underlying endothelial cell behaviour in human diseases such as tumoral angiogenesis and metastasis, inflammation or atherosclerosis, with the long term goal of manipulating these cascades for therapeutical intervention. (u-bordeaux.fr)
Formation1
  • Required for the apical junction formation of keratinocyte cell-cell adhesion. (cusabio.com)
Elastic1
  • Each fascicle is bound by an endotendineum, which is a delicate loose connective tissue containing thin collagen fibrils [1] [2] and elastic fibers. (handwiki.org)
Substrate4
  • Stress fibers were oriented about 60° to the direction of stretching on the flat substrate, while stress fibers were not aligned in any direction for the micropillar substrate. (fujipress.jp)
  • After 6 h of stretching, stress fibers on the micropillar substrate were oriented approximately 90° to the direction of stretching. (fujipress.jp)
  • These results suggest that strain in contact area between FAs and the substrate may have an impact on reorientation rates of stress fibers in ECs in response to cyclic stretching. (fujipress.jp)
  • Our study demonstrates a physical mechanism of collective durotaxis, through both cell-cell and cell-substrate adhesion ligands, based on the wetting properties of active droplets. (ibecbarcelona.eu)
Collagen1
Nano1
  • Endothelial coverage after 1, 3, and 7 days of culture on flat samples (2D model) was higher on Nano prostheses as compared with Micro scaffolds. (biomedcentral.com)
Behaviour1
  • 11] D. Lehnert, B. Wehrle-Haller, C. David, U. Weiland, C. Ballestem, B. A. Imhof, and M. Bastmeyer, "Cell Behaviour on Micropatterned Substrata: Limits of Extracellular Matix Geometry for Spreading Adhesion," J. Cell Sci. (fujipress.jp)
Surface2
  • BACKGROUND: In order to play different roles in vascular functions as a mechanosensor to blood flows and as a barrier to transvascular exchange, the endothelial surface glycocalyx (ESG) should have an organized structure. (semcs.net)
  • Regardless of the approach chosen, the surface of the prosthesis must enhance the adhesion and proliferation of ECs, to enable restoration of a functional endothelium and, as a result, to reduce clotting processes. (biomedcentral.com)
Functional1
  • In all these areas, the interrelationships of rheological properties of the systems or materials investigated and their structural and functional aspects are stressed. (semcs.net)
Effects1
Role1
  • Ongoing projects aim at demonstrating the existence of podosomes in vivo and determine their role in endothelial cell (patho)physiology. (u-bordeaux.fr)
Diseases1
  • Her current research focuses on endothelial cell biology in health and diseases. (u-bordeaux.fr)