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  • stem cell
  • A detailed knowledge of these events is important since the success of BM and stem cell transplantations depends on the efficient seeding of grafted cells in the recipient's BM. (rupress.org)
  • In 2003, however, the presence of resident cardiac stem cell was reported [ 1 ], and the shift in paradigm occurred. (hindawi.com)
  • These are mainly: mesenchymal stem cells, endothelial progenitor cells and hematopoietic stem cell. (clinicaltrials.gov)
  • 1 However, it has recently been shown that a decrease in stem cell function plays a primary role in the pathogenesis of multiple diseases and tissue aging. (ahajournals.org)
  • Sara Rankin is Professor of Leukocyte and Stem Cell Biology at the National Heart and Lung Institute, Imperial College London. (imperial.ac.uk)
  • She subsequently obtained a Wellcome Trust University award and is now a Professor in Leukocyte and Stem Cell Biology. (imperial.ac.uk)
  • This patent has broad-reaching effects, covering research, cellular therapy development and stem cell banking. (cnbc.com)
  • Large-scale applications, such as cellular therapeutic development as well as stem cell banking, require automation for parallel processing of samples from many donors. (cnbc.com)
  • The patent positions CDI as the leading supplier of choice for researchers needing a reliable and high quality supply of human cells for research, cellular therapy and stem cell banking. (cnbc.com)
  • The issuance of this patent helps assure our customers that CDI will be able to provide them access to manufactured human cells in the quantity, quality, purity and diversity that meets their drug discovery, stem cell banking and cell therapy research and development needs. (cnbc.com)
  • This patent uniquely enables CDI to process multiple cell lines simultaneously as needed to create new stem cell banks or to expand existing ones. (cnbc.com)
  • CDI was founded in 2004 by Dr. James Thomson, a pioneer in human pluripotent stem cell research at the University of Wisconsin-Madison. (cnbc.com)
  • These cells have been designated CSC based on their stem cell-like properties: self-renewal, tumorigenesis, and the ability to recapitulate a heterogeneous tumor. (frontiersin.org)
  • The CSC theory is based on our understanding of embryological development and stem cell-derived organogenesis, where a few specific cells are capable of asymmetric division leading to the generation of diverse progenitor cells responsible for the creation of complex and heterogeneous organs. (frontiersin.org)
  • Regulation of hematopoietic stem cell release, migration, and homing from the bone marrow (BM) and of the mobilization pathway involves a complex interaction among adhesion molecules, cytokines, proteolytic enzymes, stromal cells, and hematopoietic cells. (hindawi.com)
  • Finally, according to the relevance of HS moieties effects on this complex medullar niche, we describe recent data that identify HS mimetics or sulfated HS signatures as new glycanic tools and targets, respectively, for hematopoietic and mesenchymal stem cell based therapeutic applications. (springer.com)
  • HSCT
  • Liver and whole blood were collected post HSCT and used for the analysis of pathology of liver sinusoidal endothelial cells (LSECs) and hepatocytes, liver ultrastructure, function, level of IL-6, TNF-α and platelet activation. (ovid.com)
  • differentiate
  • Although this cell type does not differentiate into myocardium, it is equipped with advantageous features with low tumorigenicity and relative tolerance for ischemia in contrast to cardiomyocytes. (hindawi.com)
  • vitro
  • In this review, we systematically present and compare the techniques that have drawn the most attention in this field and that generally have focused on four important issues: the scaffold material selection, the scaffold material production, cellular selection and in vitro cell culture. (scielo.br)
  • and (4) in vitro cell culture. (scielo.br)
  • Retroviral and lentiviral vectors have been extensively used for genetic modification of cells in vitro and in vivo due to the efficiency with which they can transduce and integrate genes into a broad array of human cell types. (bloodjournal.org)
  • This can be performed in vitro with certain types of human cells with significant efficiency and selectivity. (bloodjournal.org)
  • 1 In vitro, these CD8-ScFv-displaying lentiviral vectors selectively transduce human CD8 + T cells and express the vector-encoded TCRs. (bloodjournal.org)
  • 8 The current study is a further advance, demonstrating not only the striking selectivity and transduction efficiency of the CD8-targeted vectors in vitro but also their capacity to selectively transduce widely distributed human CD8 + T cells in vivo. (bloodjournal.org)
  • For the immunocompromised cancer patient or transplant recipient with a drug-resistant or otherwise untreatable life-threatening viral infection, in vitro or, particularly, in vivo transduction of CD8 + T cells to express TCRs specific for epitopes of critical viral proteins known to be presented by 1 or more HLA alleles of the patient, could induce rapid development of meaningful resistance. (bloodjournal.org)
  • tissues
  • Parallel contribution of the endothelial selectins and VCAM-1 is not known to direct blood cell trafficking to other noninflamed tissues. (rupress.org)
  • The primary defects were restricted to extraembryonic tissues, namely the yolk sac vasculature and haematopoietic system. (biologists.org)
  • Isolation and molecular characterization of rare cells ( e.g. circulating tumor and stem cells) within biological fluids and tissues has significant potential in clinical diagnostics and personalized medicine. (mcponline.org)
  • Under steady-state conditions, a small amount of hematopoietic stem cells constantly leave the BM and penetrate tissues, returning to the BM or peripheral niches via the blood or lymphatic system [ 2 ]. (hindawi.com)
  • HS associated to core protein, constitute the superfamily of heparan sulfate proteoglycans (HSPGs) present on the cell surface and in the extracellular matrix of all tissues. (springer.com)
  • Skeletal
  • We studied trogocytosis in the interaction of T cells with human skeletal muscle cells modeling muscle-immune cell interactions under pathophysiological conditions i.e. myositis. (biomedsearch.com)
  • neurons
  • This study aimed at evaluating whether apoptosis of interstitial cells of Cajal (ICC), smooth muscle cells (SMC), and enteric neurons was involved in a guinea pig model of intestinal ischemia and reperfusion injury. (biomedsearch.com)
  • coronary artery
  • 7,9 In addition to age, which is known to affect stem cells functions, risk factors for coronary artery disease (CAD) and heart failure diminish the capacity of the bone marrow-derived cell to contribute to functional repair ( Figure 1 ). (ahajournals.org)
  • Impaired CXCR4 signaling contributes to the reduced neovascularization capacity of endothelial progenitor cells from patients with coronary artery disease," Circulation Research , vol. 97, no. 11, pp. 1142-1151, 2005. (hindawi.com)
  • isolation
  • 2 , 3 Attempts to selectively transduce specific types of cells have largely focused on isolation of those cells before exposure to the vector. (bloodjournal.org)
  • Microfluidic magnetophoretic isolation of target cells spiked into 1 ml of blood at the level of 1000-2000 cells/ml, followed by focused acoustics-assisted sample preparation has been coupled with one-dimensional PLOT-LC-MS methodology. (mcponline.org)
  • The characterization of rare cells in limited volumes of physiological fluids is shown by the isolation and quantitative proteomic profiling of first MCF-7 cells spiked into whole blood as a model system and then two CD133+ endothelial progenitor and hematopoietic cells in whole blood from volunteers. (mcponline.org)
  • Additionally, the isolation of adult stem cells provides a needed cell source for tissue engineering and regenerative medicine treatments ( 5 , 6 ). (mcponline.org)
  • gene expression
  • gene expression is DIAPH2 colinear with the AP axis in very early development: the 3' genes are expressed in anterior anatomical structures while 5' genes are expressed in posterior structures [3 4 Later in development genes participate in organogenesis and numerous observations suggest that genes modulate different stages of hematopoietic development. (bio-naturlan.com)
  • HPCs
  • However, little is known about the adhesion pathways that are involved in the homing of hematopoietic stem cells and hematopoietic progenitor cells (HPCs) from the blood to the BM. (rupress.org)
  • It is known that L-selectin is expressed on the majority of HPCs (( 9 )-( 11 )), but its function on these cells is unclear. (rupress.org)
  • HPCs were also shown to bind to P-selectin (( 13 )), although the expression of P-selectin on BM endothelial cells has not been described. (rupress.org)
  • EC incubation with IL-6 did not improve cell expansion in comparison to non-stimulated EC supernatant, while the HPCs' phenotype and functional capacities were retained. (nih.gov)
  • mesoderm
  • Retinoic acid solution (RA) is definitely a more developed inducer of genes during development of neurectoderm however ramifications of RA about expression are poorly described in mesoderm rather than described in the hematopoietic compartment. (bio-naturlan.com)
  • Pulses of RA exposure demonstrate that the complexes are decoupled from RA regulation progressively in lateral plate mesoderm as it undergoes hematopoietic specification. (bio-naturlan.com)
  • Most of these studies focus on the embryonic development of neurectoderm while RA-dependent regulation of genes in mesoderm and particularly within the hematopoietic compartment has been assumed but never tested comprehensively. (bio-naturlan.com)
  • cardiovascular
  • Pretreatment of cells or the target tissue by small molecules, polymers, growth factors, or a combination thereof may provide useful approaches for enhancement of cell therapy for cardiovascular diseases. (ahajournals.org)
  • The possibility of preserving or even rejuvenating endothelial function offers a potent means of preventing/treating some of the most fearful aspects of aging such as loss of mental, cardiovascular, and sexual function. (aidanproducts.com)
  • cardiomyocytes
  • The cell size of cardiomyocytes was also studied. (scielo.br)
  • In the case of myocardial infarction, hypertrophic responses occur in cardiomyocytes in the surviving portion of the ventricle, and ventricular dilatation follows as a result of re-organization of the myocardium, including cardiomyocytes and mesenchymal cells in combination (2,3). (scielo.br)
  • chemoattractant
  • Subsequently, when a rolling cell encounters a chemoattractant that binds to surface receptors, an intracellular signaling cascade is triggered that induces the functional upregulation of integrins. (rupress.org)
  • self-renewal
  • Stem cells were considered to possess unlimited self-renewal capacity and to be able to replace themselves throughout the lifespan of the organism. (ahajournals.org)
  • Similarly, the CSC theory explains that there exists a hierarchy of cells, where CSC are capable of unregulated asymmetric division, which is responsible for self-renewal and generation of a diverse population of differentiated progenitor cells that ultimately make up a heterogeneous tumor. (frontiersin.org)
  • human
  • Currently, most lentiviral vectors used for genetic modification of human cells are packaged or pseudotyped in an envelope protein derived from vesicular stomatitis virus (VSV) that is able to bind to and facilitate entry into a broad array of human cells. (bloodjournal.org)
  • Aug. 28, 2014 (GLOBE NEWSWIRE) -- Cellular Dynamics International (CDI) (Nasdaq:ICEL) today announced that the U.S. Patent and Trademark Office (PTO) awarded the company Patent No. 8,815,585 on the automated production of human pluripotent stem cells, including induced pluripotent stem cells (iPSCs). (cnbc.com)
  • U.S. Patent No. 8,815,585 covers the automated production of human pluripotent stem cells, including induced pluripotent stem cells (iPSCs). (cnbc.com)
  • CDI manufactures human iPSCs and differentiated cells at industrial scale and to tight specifications. (cnbc.com)
  • Bob Palay, chief executive officer of CDI, said, "CDI has distinguished itself as the leader of human cell manufacturing based on our industrialized process of manufacturing iPSCs and differentiated cells. (cnbc.com)
  • We believe that automation is critical to both large-scale manufacture of human stem cells and differentiated cell types, and their application, especially in cellular therapeutics. (cnbc.com)
  • Cellular Dynamics International, Inc. (CDI) is a leading developer and manufacturer of fully functioning human cells in industrial quantities to precise specifications. (cnbc.com)
  • CDI's proprietary iCell Operating System (iCell O/S) includes true human cells in multiple cell types (iCell products), human induced pluripotent stem cells (iPSCs) and custom iPSCs and iCell products (MyCell Products). (cnbc.com)
  • When the differentiated cells were incubated overnight in human serum at 37°C, E. coli or PMA induced a ten-fold burst, whereas no effect was seen in response to fMLP (Figure 4B). (nih.gov)
  • Burst rates between cells, which had been stored overnight in human serum and those without serum incubation were significantly different (p ≤ 0.018). (nih.gov)
  • vessels
  • FDCP-mix cell rolling was measured in the same BM vessels before and after injection of anti-P- and/or anti-E-selectin mAbs (100 μg/mouse) or treatment of cells with anti- L-selectin mAb (100 μg/10 7 cells). (rupress.org)