• Several cardiovascular diseases are characterized by endothelial cell activation or dysfunction triggered by external stimuli such as disturbed flow, hypoxia, growth factors, and cytokines in response to high levels of low-density lipoprotein and cholesterol, hypertension, diabetes, aging, drugs, and smoking. (nih.gov)
  • To distinguish these mechanisms, human aortic endothelial cells (HAECs) were stimulated with TNFα and exposed to pro-remodeling or vasoprotective shear stress profiles. (degruyter.com)
  • Low passage porcine aortic endothelial cells were incubated with chrysotile (10 µ1g/cm2) or crocidolite asbestos (5 µ1g/cm2) or with refractory ceramic fiber 1 (RCF-I) (10 µ1g/cm2) for up to 24 hours and then assayed for steady state uPA mRNA levels by solution hybridization or uPA enzymatic activity by in situ or gel-based zymography. (cdc.gov)
  • The physiological functions of endothelial cells control vascular tone, permeability, inflammation, and angiogenesis, which significantly help to maintain a healthy vascular system. (nih.gov)
  • ELTD1 is expressed in endothelial and vascular smooth muscle cells and has a role in angiogenesis. (ox.ac.uk)
  • Circulating endothelial progenitor cells (EPCs) are increased in conditions associated with ischaemia and can potentially support angiogenesis and vasculogenesis. (bmj.com)
  • To better understand the role of LRP1 in endothelial cell function, we investigated how LRP1 regulates mouse retinal angiogenesis. (nih.gov)
  • 5. Low-Intensity Pulsed Ultrasound Treatment Accelerates Angiogenesis by Activating YAP/TAZ in Human Umbilical Vein Endothelial Cells. (nih.gov)
  • Acquired expression of periostin by human breast cancers promotes tumor angiogenesis through up-regulation of vascular endothelial growth factor receptor 2 expression. (nih.gov)
  • Tumor cell lines engineered to overexpress periostin showed a phenotype of accelerated growth and angiogenesis as xenografts in immunocompromised animals. (nih.gov)
  • The underlying mechanism of periostin-mediated induction of angiogenesis was found to derive in part from the up-regulation of the vascular endothelial growth factor receptor Flk-1/KDR by endothelial cells through an integrin alpha(v)beta(3)-focal adhesion kinase-mediated signaling pathway. (nih.gov)
  • Pulmonary arterial hypertension (PAH) is a rare disease characterized by a pro-proliferative endothelial cell phenotype, vascular inflammation and pulmonary vessel remodeling culminating in right ventricular failure and ultimately death. (nih.gov)
  • Other linked pathological processes include acute and chronic inflammation, stasis, reperfusion injury, endothelial activation and dysfunction, coagulation activation, and excess oxidant stress. (nih.gov)
  • Using this model, we demonstrate that macrophage-mediated inflammation is regulated by endothelial cell activity. (nature.com)
  • Furthermore, endothelial inflammation occurs independently of macrophages following exposure to CAR T-cell products and the induced endothelial inflammation potentiates macrophage-mediated inflammatory signaling, leading to a hyperinflammatory environment. (nature.com)
  • Lastly, we demonstrate how targeting STAT3 activity can abrogate endothelial inflammation and attenuate this otherwise hyperinflammatory environment. (nature.com)
  • 8. Attenuation of antimalarial agent hydroxychloroquine on TNF-α-induced endothelial inflammation. (nih.gov)
  • 11. Luteolin protects against vascular inflammation in mice and TNF-alpha-induced monocyte adhesion to endothelial cells via suppressing IΚBα/NF-κB signaling pathway. (nih.gov)
  • Project 2 will examine mechanisms of redox regulation of cell signaling in vascular function and how these processes are perturbed by endothelial cell exposure to oxidants and reactive lipids that are associated with inflammation and metabolic disease. (nih.gov)
  • Project 3 will examine the functional interplay between endothelial function and inflammation in adipose tissue and assess how these processes influence systemic metabolism by focusing on mouse models that over- and under-express the adipocyte-derived cytokine adiponectin. (nih.gov)
  • Cathepsins K and V are powerful elastases elevated in endothelial cells by tumor necrosis factor-α (TNFα) stimulation and disturbed blood flow both of which contribute to inflammation-mediated arterial remodeling. (degruyter.com)
  • In conclusion, NF-κB and AP-1 activation were necessary to activate cathepsin K expression in endothelial cells, highlighting integration of biochemical and biomechanical stimuli to control cathepsins K and V, powerful elastases implicated for arterial remodeling due to chronic inflammation and disturbed blood flow. (degruyter.com)
  • In order to provide a rational direction for future research, we also compiled the most recent data regarding the impact of endothelial cell dysfunction on vascular diseases and potential targets that impede the pathogenic process. (nih.gov)
  • A decrease in EPC levels was shown to correlate inversely with atherosclerotic risk factors 11 and the occurrence of ischaemic stroke, 12 and to correlate with endothelial dysfunction, 11, 13 accelerated atherosclerosis 14 and in-stent restenosis. (bmj.com)
  • ABSTRACT This study assessed changes in serum levels of cytokines IFNγ and IL-10 (biomarkers of inflammatory changes) and soluble biomarkers sICAM-1 and sE-selectin (biomarkers of endothelial dysfunction) in diabetic patients with and without nephropathy. (who.int)
  • Clinical emergence of endothelial dysfunction, characterized by increased pro-inflammatory signaling (e.g. (nature.com)
  • Endothelial dysfunction is also highly correlated with macrophage-derived systemic inflammatory markers descriptive of CRS suggesting the endothelium contributes to its pathogenesis as well 2 , 21 . (nature.com)
  • Despite this growing body of evidence, the underlying pathophysiologic mechanisms leading to endothelial dysfunction, its contribution to the hyperinflammatory environment, and the interplay of the vascular endothelium with inflammatory macrophages, has yet to be explored. (nature.com)
  • Endothelial progenitor cells (EPCs) 7 reside in the bone marrow and are found to some extent in the peripheral blood. (bmj.com)
  • 9. Tanshinone II A Attenuates TNF-α-Induced Expression of VCAM-1 and ICAM-1 in Endothelial Progenitor Cells by Blocking Activation of NF-κB. (nih.gov)
  • We identified KDR+/CD34high/CD144+/CD43-/CD45- hemato-endothelial progenitor cells (HEPs) forming organized, vasculature-like structures and giving rise to CD34low/CD144-/CD43+/CD45+ hematopoietic progenitor cells. (haematologica.org)
  • Thus, our data suggest an important role for IL-3 in early human hematopoiesis by supporting the endothelial to hematopoietic transition of hemato-endothelial progenitor cells and highlight the potential of a hemanoid-based model to study human hematopoietic development. (haematologica.org)
  • Here, we hypothesized that CAV1 loss-of-function in the pulmonary artery endothelium leads to a hyperproliferative, pro-inflammatory phenotype that contributes to the pathogenic pulmonary vascular remodeling seen in patients with PAH. (nih.gov)
  • Increased endothelial proliferation as detected by Ki67 staining was observed in LRP1-deleted retinal endothelium in response to hypoxia. (nih.gov)
  • Abstract: DESCRIPTION (provided by applicant): It is widely recognized that the endothelium is affected by the metabolic state of the organism, and endothelial function can also influence systemic metabolism. (nih.gov)
  • Vascular smooth muscle cells (SMCs), upon endothelium damage, transit from contractile phenotype to synthetic phenotype. (rochester.edu)
  • Inhalation of asbestos may activate the pulmonary endothelium to promote an altered cell phenotype that participates in the development of pulmonary fibrosis. (cdc.gov)
  • Angiosarcomas Express Mixed Endothelial Phenotypes of Blood and Lymphatic Capillaries: Podoplanin as a Specific Marker for Lymphatic Endothelium," American Journal of Pathology, Vol. 154, 1999, pp. 385-394. (scirp.org)
  • SLC4A11 mutations in Fuchs endothelial corneal dystrophy. (medscape.com)
  • Linkage of a mild late-onset phenotype of Fuchs corneal dystrophy to a novel locus at 5q33.1-q35.2. (medscape.com)
  • Fuchs endothelial corneal dystrophy (FECD) is an eye disease. (nih.gov)
  • When Do Symptoms of Fuchs endothelial corneal dystrophy Begin? (nih.gov)
  • 2. Flow-dependent YAP/TAZ activities regulate endothelial phenotypes and atherosclerosis. (nih.gov)
  • Distinct endothelial phenotypes evoked by arterial waveforms derived from atherosclerosis-susceptible and -resistant regions of human vasculature. (degruyter.com)
  • Here we report a novel in vitro model of the macrophage-endothelial interface to study the effects of CAR T-cell-induced cytokine storm. (nature.com)
  • Utilizing a network model, coupled to in vitro secretion profiling, we identified STAT3 programming as critical in regulating this endothelial behavior. (nature.com)
  • Leukocytes have also been shown to migrate through single endothelial cells both in vitro and in vivo [ 18 , 19 ]. (hindawi.com)
  • Instruction of circulating endothelial progenitors in vitro towards specialized blood-brain barrier and arterial phenotypes. (eurogentec.com)
  • Increasing evidence suggests that uncontrolled proinflammatory signaling and further alteration in endothelial cell phenotypes such as barrier disruption, increased permeability, endothelial to mesenchymal transition (EndMT), and metabolic reprogramming further induce vascular diseases, and multiple studies are focusing on finding the pathways and mechanisms involved in it. (nih.gov)
  • Expression and consequently ligand independent activation of ELTD1 results in endothelial-mesenchymal transistion (EndMT) with a loss of cell-cell contact, formation of stress fibres and mature focal adhesions and an increased expression of smooth muscle actin. (ox.ac.uk)
  • Fibrosis is associated with the emergence of fibroblasts derived from endothelial cells, suggesting an endothelial-mesenchymal transition (EndMT). (biolegend.com)
  • Together, these data reveal a pivotal role for LRP1 in endothelial cell proliferation and retinal neovascularization induced by hypoxia. (nih.gov)
  • However, the relationship between CAV1 loss-of-function and the dysfunctional pulmonary vascular phenotype in PAH is unknown. (nih.gov)
  • In primary, human pulmonary artery endothelial cells (PAECs), CAV1 was knocked down using siRNA approach. (nih.gov)
  • Raf/ERK drives the proliferative and invasive phenotype of BMPR2-silenced pulmonary artery endothelial cells. (nih.gov)
  • Project 5 will examine the role of mitochondrial homeostasis in endothelial and inflammatory cells isolated from patients with Type 2 diabetes mellitus. (nih.gov)
  • More recently, Zeldin's group has developed several transgenic mice with endothelial-specific expression of human CYP2C8 or CYP2J2 to investigate the role of endothelial EETs on cardiovascular function. (nih.gov)
  • Nowadays, more and more studies have revealed that cells in the cardiovascular system (such as cardiomyocytes, vascular smooth muscle cells, endothelial cells, et al. (frontiersin.org)
  • Recent findings indicate a complex interaction between kinases, phosphatases, and the adherens junction components that allow a fine regulation of the endothelial permeability to small molecules, leukocyte migration, and barrier resealing. (hindawi.com)
  • Specifically, retinas in mice lacking endothelial LRP1 have more branching points and angiogenic sprouts at the leading edge of the newly formed vasculature. (nih.gov)
  • Vehicle-exposed Chd8V986*/+ mice displayed ASD-associated phenotypes, including anxiety-like behavior and altered sociability, replicating a previous study with this mouse line. (nih.gov)
  • Moreover, oligodendrocyte and neurodegenerative transcriptional phenotypes were apparent in 12 and 18 month old Chd8V986*/+ mice. (nih.gov)
  • Following DM exposure, the mutant mice displayed an exacerbated phenotype in the elevated plus maze, and genes associated with vascular endothelial cells were downregulated in the cerebral cortex of older Chd8V986*/+ animals. (nih.gov)
  • VE-cadherin is critical for endothelial biology and is required for vessel maturation in multiple species ranging from zebrafish [ 7 ] to mice [ 8 , 9 ]. (hindawi.com)
  • Critical role of endothelial cell activation in hypoxia-induced vasoocclusion in transgenic sickle mice. (degruyter.com)
  • Gene set enrichment analysis of the data identified a number of pathways involved in myofibroblast biology suggesting that the endothelial cells had undergone a type II EMT. (ox.ac.uk)
  • Two pathways mediate the passage across the endothelial barrier. (hindawi.com)
  • With these established methods, we further studied the role of endothelial cells (ECs) and their molecular pathways in inducing EP maturation of primitive hESC-CMs. (ca.gov)
  • We combine index sorting of single cells from the embryonic aorta-gonad-mesonephros region with endothelial cell co-culture and transplantation to characterize the phenotypic properties and engraftment potential of single hematopoietic precursors. (jove.com)
  • BACKGROUND: Assessment of endothelial colony-forming cell (ECFC) number and vasculogenic properties is crucial for exploring vascular diseases and regeneration strategies. (imperial.ac.uk)
  • We demonstrate that the endothelial to hematopoietic transition of HEPs is dependent on the presence of interleukin 3 (IL-3). (haematologica.org)
  • 3-5 The emergence of hematopoietic cells from the HE (also referred to as endothelial to hematopoietic transition [EHT]), is controlled by distinct cell-intrinsic signals mediated by transcription factor (TF) activation, as well as important cell-extrinsic factors such as cell-cell interactions, extracellular matrix molecules, and cytokines. (haematologica.org)
  • 7 Conditions that induce tissue ischaemia or endothelial damage-that is, vascular trauma, 8 acute myocardial infarction 9 and unstable angina 10 -are associated with a release of angiogenic factors, including vascular endothelial growth factor (VEGF) and erythropoietin, which promote mobilisation of EPC from the bone marrow to the peripheral circulation. (bmj.com)
  • We overexpressed FSTL3 in iPS-ECs and found that FSTL3 could induce and enhance endothelial features by facilitating β-catenin nuclear translocation through inhibition of glycogen synthase kinase-3β activity and induction of Endothelin-1. (qub.ac.uk)
  • Senescence-associated secretory phenotype (SASP) is a phenotype associated with senescent cells wherein those cells secrete high levels of inflammatory cytokines, immune modulators, growth factors, and proteases. (wikipedia.org)
  • Cellular senescence is a process in which various stressors permanently stop cell division, resulting in cell changes that appear as senescent-associated secretory phenotype (SASP) ( 2 ). (medsci.org)
  • Cultured human umbilical vein endothelial cells (HUVECs) were exposed to H 2 O 2 as oxidative stress and a high glucose concentration with palmitate as a glucolipotoxic condition. (medsci.org)
  • 7. Sargaquinoic Acid Inhibits TNF-α-Induced NF-κB Signaling, Thereby Contributing to Decreased Monocyte Adhesion to Human Umbilical Vein Endothelial Cells (HUVECs). (nih.gov)
  • Paradigm shifts in the field will be presented including the discovery of unique metabolic requirements of lymphatic endothelial cells and the role of brain lymphatics in neurodegenerative disease. (grc.org)
  • We studied tumour lymphangiogenesis and lymphatic invasion using D2-40 endothelial marker in 35 breast cancer patients treated by primary surgery and correlated it with various clinico-pathological prognostic parameters. (scirp.org)
  • M. A. Ansari, V. Pandey, V. Srivastava, M. Kumar, R. Mishra and A. Kumar, "Lymphangiogenesis as a Prognostic Marker in Breast Cancer Using D2-40 as Lymphatic Endothelial Marker-A Preliminary Study," Journal of Cancer Therapy , Vol. 3 No. 5A, 2012, pp. 814-821. (scirp.org)
  • 3. L-tetrahydropalamatine inhibits tumor necrosis factor-α-induced monocyte-endothelial cell adhesion through downregulation of intercellular adhesion molecule-1 and vascular cell adhesion molecule-1 involving suppression of nuclear factor-κ B signaling pathway. (nih.gov)
  • Shing Y, Folkman J, Sullivan R, Butterfield C, Murray J, Klagsbrun M. Heparin affinity: purification of a tumor-derived capillary endothelial cell growth factor. (childrenshospital.org)
  • Depletion of LRP1 in endothelial cells results in increased retinal neovascularization in a mouse model of oxygen-induced retinopathy. (nih.gov)
  • Using an array of biochemical and cell biology approaches, we demonstrate that poly(ADP-ribose) polymerase-1 (PARP-1) directly interacts with LRP1 in human retinal microvascular endothelial cells. (nih.gov)
  • While corticosteroids, the current gold standard of care, attenuate the resulting macrophage inflammatory signaling, the endothelial activity remains refractory to this treatment strategy. (nature.com)
  • At the placentation site, TCDD activated AHR signaling within endothelial cells but not trophoblast cells. (nih.gov)
  • 12. Rabbit sera containing compound danshen dripping pill attenuate leukocytes adhesion to TNF-alpha--activated human umbilical vein endothelial cells by suppressing endothelial ICAM-1 and VCAM-1 expression through NF-kappaB signaling pathway. (nih.gov)
  • Suppression of Tumour Lymphangiogenesis and Lymph Node Metastasis by Blocking Vascular Endothelial Growth Factor Receptor 3 Signaling," Journal of the National Cancer Institute, Vol. 94, No. 11, 2002, pp. 819-825. (scirp.org)
  • Influence of the oxygen microenvironment on the proangiogenic potential of human endothelial colony forming cells. (eurogentec.com)
  • Magnesium deficiency promotes a pro-atherogenic phenotype in cultured human endothelial cells via activation of NFkB. (thieme-connect.de)
  • Modern resources like the UK Biobank (UKB, www.ukbiobank.ac.uk ) that make thousands of phenotypes available to match these genetic data are proving a boon to our understanding of human genetics. (nature.com)
  • Anterior keratocyte depletion in fuchs endothelial dystrophy. (medscape.com)
  • Fuchs' endothelial dystrophy of the cornea. (medscape.com)
  • Quality of vision in patients with fuchs endothelial dystrophy and after descemet stripping endothelial keratoplasty. (medscape.com)
  • Treatment of Fuchs Endothelial Dystrophy by Descemet Stripping Without Endothelial Keratoplasty. (medscape.com)
  • Fiber-specific activation of endothelial cells to express more active uPA may contribute to the tissue remodeling cascade seen following asbestos fiber deposition within the lung. (cdc.gov)
  • Our study reveals a gene x environment interaction with a Chd8 haploinsufficient mouse line and points to the importance of investigating phenotypes in ASD animal models across the lifespan. (nih.gov)
  • After using our rare-variant-tailored methodology to reduce test statistic inflation, we identify 64 statistically significant gene-based associations in our meta-analysis of the two cohorts and 37 for phenotypes available in only one cohort. (nature.com)
  • 4. Clematichinenoside inhibits VCAM-1 and ICAM-1 expression in TNF-α-treated endothelial cells via NADPH oxidase-dependent IκB kinase/NF-κB pathway. (nih.gov)
  • 18. Danshenol A inhibits TNF-α-induced expression of intercellular adhesion molecule-1 (ICAM-1) mediated by NOX4 in endothelial cells. (nih.gov)
  • The fight against vascular disease requires functional endothelial cells (ECs) which could be provided by differentiation of induced Pluripotent Stem Cells (iPS Cells) in great numbers for use in the clinic. (qub.ac.uk)
  • After three weeks of culture, only 0.1% of cells exhibited the input HSC phenotype, but these accounted for almost all functional long-term HSC activity. (elifesciences.org)
  • Developmental pyrethroid exposure and age influence phenotypes in a Chd8 haploinsufficient autism mouse model. (nih.gov)
  • However, mechanisms behind endothelial cell integration of biochemical and biomechanical cues to regulate cathepsin production are not known. (degruyter.com)
  • 1. TNF-α-Induced YAP/TAZ Activity Mediates Leukocyte-Endothelial Adhesion by Regulating VCAM1 Expression in Endothelial Cells. (nih.gov)
  • 6. ZLJ-6, a novel COX/5-LOX inhibitor, attenuates TNF-α-induced endothelial E-selectin, ICAM-1 and VCAM-1 expression and monocyte-endothelial interactions via a COX/5-LOX-independent mechanism. (nih.gov)
  • 13. Elevated ZIPK is required for TNF-α-induced cell adhesion molecule expression and leucocyte adhesion in endothelial cells. (nih.gov)
  • Statins upregulate KLF2 expression in endothelial cells, and this activity is associated with an antiinflammatory phenotype. (jci.org)
  • Project 1 will examine the mechanism by which endothelial cells switch from aerobic respiration to anaerobic glycolysis under conditions that stimulate vascular growth (i.e. hypoxia and pseudo-hypoxia) by focusing on a HIF1a-regulated microRNA that regulates the expression of mitochondrial respiratory complex proteins. (nih.gov)
  • 0.1%) variants against 4264 phenotypes in 49,960 exome-sequenced individuals from the UK Biobank and 1934 phenotypes (1821 overlapping with UK Biobank) in 21,866 members of the Healthy Nevada Project (HNP) cohort who underwent Exome + sequencing at Helix. (nature.com)
  • Our results demonstrate that endothelial cells play a central role in the pathophysiology of CAR T-cell toxicities and targeting the mechanisms driving the endothelial response can guide future clinical management. (nature.com)
  • Endothelial cells play a critical role in the regulation of vasoreactivity, hemostasis, and leukocyte recruitment. (hindawi.com)
  • We identified that Follistatin-Like 3 (FSTL3) is highly expressed in iPS-ECs, and, as such, we sought to clarify its possible role in retaining and improving iPS-ECs function and phenotype, which are crucial in increasing the cells' potential as a therapeutic tool. (qub.ac.uk)
  • This review highlights the main proinflammatory stimuli and their effects on endothelial cell function. (nih.gov)
  • While it is often presumed that all non-specialized endothelial cells are the same, and thus will have the same response to various pharmacological agents, disease states, and stimuli, this may not be true. (umsystem.edu)