Endothelial cells in culture tumor cells cultured. Medical search. Frequent questions

Activation of the VEGFA/VEGFR2 signaling and expression of blood vascular endothelial cell (BEC) markers, such as NRP1 and EphrinB2, further differentiate these precursor cells into BECs, which then form the hierarchical network of blood vessels 4 . (nature.com)

VEGF, produced in large amounts by cancer cells during tumor growth, interacts with its receptors VEGFR1 and VEGFR2 thereby causing endothelial cell survival, proliferation, and sprouting [7] . (plos.org)
Knockdown of KDM1B inhibits cell proliferation and induces apoptosis of pancreatic cancer cells. (cancerindex.org)
However, it increases proliferation rates in some cell types (e.g. mesenchymal stem cells, osteosarcoma cells, HUVECs) which puts extra demands on nutrient supply and waste removal [1,2,3] . (reprocell.com)
The systems have been shown to improve cell proliferation and specific protein expression beyond that seen for static 3D cultures [4,5,6,7] . (reprocell.com)
The levels of glucose and secreted lactate contained in the cell culture medium were monitored as indicators of metabolic depletion, while protein syntheses and cell proliferation were measured by expression of phosphorylated Ribosomal protein S6 and cyclin D1. (reprocell.com)
Results suggested that perfusion culture reduces hypoglycemia, anaerobic stress, and preserves protein synthesis and cell proliferation. (reprocell.com)
This study investigated the effect of acylated synthetic ghrelin (AG) on the survival and proliferation of human chemosensitive ovarian cancer cells (A2780) and explored some mechanisms of action with a focus on the p53 apoptotic pathway and PI3K/Akt and NF- κ B survival pathways. (hindawi.com)
However, while AG treatment had no effect on control cell viability, it significantly increased cell viability and proliferation and inhibited cell death in Cis-treated cells. (hindawi.com)
Among them and over decades of research, it was shown that the PI3K/Akt/mTOR pathway is one of the most frequently hyperactive pathways in OC as well as other types of solid tumors and is associated with increased cell growth, proliferation, migration, protein synthesis, survival, and chemotherapy resistance [ 7 - 9 ]. (hindawi.com)
1994. Mesothelial cell proliferation: A nonspecific response to lung injury associated with fibrosis. (cdc.gov)

As cells are capable of sensing and responding to the stiffness of their surrounding matrix via mechanisms that include changes in the phosphorylation of focal adhesion proteins, like FAK and Src, we hypothesized that there is a relationship between abnormal tumor vasculature characteristics and tumor stiffening. (cornell.edu)
Induced EndoMT cells exhibited up-regulation of mesenchymal markers, including collagen type I and α-smooth muscle actin, and a reduction in endothelial cell and junctional proteins, including von Willebrand factor, CD31, occludin, and vascular endothelial-cadherin. (surrey.ac.uk)

CSCs are enriched near macrophages, particularly near macrophage-containing intravasation sites called Tumor Microenvironment of Metastasis (TMEM) doorways. (nature.com)
In this study, we report the 3D reconstitution of normal bladder and patient-specific bladder tumour "assembloids" - organoids derived from tissue stem/tumour cells reconstituted with other components of the tissue stroma/microenvironment such as stromal fibroblasts, endothelial cells, and muscle cells. (researchsquare.com)
It is well established that the role of the tumor microenvironment (TME) in cancer progression and therapeutic resistance is crucial, but many of the underlying mechanisms are still being elucidated. (frontiersin.org)
The mixture of conditions in the microenvironment exemplified by hypoxia-induced ovarian cancer TNTs playing a crucial role in tumor growth, as just one example, is a potential avenue of investigation that will uncover their role in relation to other known factors, including EVs. (frontiersin.org)
The tumor microenvironment (TME) is lush with a complex array of interacting parts. (frontiersin.org)

In vitro , the sprouting and migratory properties of primary endothelial cell cultures were reduced by inhibiting EZH2 through up-regulation of miR-101, siRNA-mediated knockdown of EZH2, or treatment with 3-Deazaneplanocin-A (DZNep), a small molecule inhibitor of EZH2 methyltransferase activity. (plos.org)
Dr. Yang has extensive expertise in developing novel biomaterials and stem cell-based therapeutics for musculoskeletal tissue engineering, or engineering 3D in vitro cancer models for drug screening and mechanistic discovery. (stanford.edu)
We determined the presence of EndoMT in the pulmonary vasculature in vivo and the functional effects on pulmonary artery endothelial cells (PAECs) undergoing EndoMT in vitro. (surrey.ac.uk)
Three-dimensional (3D) in vitro culture can improve the performance and physiological relevance of immortalized and primary cells. (reprocell.com)
If this is the kind of results you want to achieve with your in vitro cell models, then adding perfusion can be done with minimal disruption to your existing protocols. (reprocell.com)
In vitro expansion of mesenchymal stem cells using 3-D matrix derived from cardiac fibroblast. (reprocell.com)
They have been imaged in vitro and also in vivo and ex vivo in tumors from human patients as well as animal models, thus not only proving their existence in the TME, but opening further speculation about their exact role in the dynamic niche of tumor ecosystems. (frontiersin.org)
Application of stretch and/or strain to in vitro tissue models also requires 3D gel-based cell culture models that are stretchable and/or compressible, which is inappropriate for silicone-based microfluidic models. (biomedcentral.com)
The aim of the present study was to identify whether P. gingivalis heat‑shock protein 60 (HSP60) induced the dysfunction of human umbilical vein endothelial cells (HUVECs) in vitro. (spandidos-publications.com)
C1QBP knockdown and overexpression cells were established via lentiviral infection and subjected to apoptosis and ROS assay in vitro . (medsci.org)

The aim of this work was to study the protective activity of a honey of multifloral origin, standardized for total antioxidant power and analytically profiled (HPLC-MS) in antioxidants, in a cultured endothelial cell line (EA.hy926) subjected to oxidative stress. (researchgate.net)
oxidative stress, intracellular calcium levels, endothelial barrier function, cell viability, and apoptosis were measured in vascular endothelial cells treated with 90 μM linoleic acid (18:2, n-6) and/or 20 ng/mL TNF (100 U/mL). (uky.edu)
Although initial treatment with TNF or 18:2 significantly increased oxidative stress and intracellular calcium levels, only exposure to TNF induced apoptosis in cultured endothelial cells. (uky.edu)
However, the role of C1QBP in metabolism, oxidative stress, and apoptosis of renal cell carcinoma (RCC) cells have not yet been explored. (medsci.org)

Macrophages are emerging as major cellular factors in the tumor ecosystem that can influence the stem phenotype and cancer progression. (nature.com)
These parts include a multitude of cell types that evolve during the course of carcinogenesis and cancer cell progression. (frontiersin.org)
Among a ll, accumulating evidence has shown that ghrelin, an endogenous ligand for growth hormone secretagogue receptor (GHSR), may play a crucial role in the development and progression of numerous kinds of solid tumors [ 10 ]. (hindawi.com)
In vivo , orthotopic tumor xenografts assay was performed to investigate the role of C1QBP in RCC progression. (medsci.org)
We propose that loss of PTEN during malignant progression contributes to tumor expansion through the deregulation of Akt activity and HIF-1-regulated gene expression. (ox.ac.uk)

Her research group is particularly interested in developing biomaterials and cell-based therapeutics to improve regeneration of various musculoskeletal tissues including bone, cartilage, tendon, blood vessels etc. (stanford.edu)
Tumor blood vasculature tends to be heterogeneously distributed, densely branched, tortuous, malformed, and hyperpermeable relative to that found within physiological tissues. (cornell.edu)
By grafting glycated collagen constructs to the chorioallantoic membranes of chicken embryos cultured ex ovo, and with two- and three-dimensional human umbilical vein endothelial cell culture systems, we show that increasing the stiffness of a tissue significantly enhances the extent of angiogenic vascularization it experiences, and that neovessels or monolayers formed within stiff tissues or on stiff substrates are significantly more permeable than their compliant counterparts. (cornell.edu)
However, some tissues are exposed to both types of mechanical stimuli, e.g. vascular endothelial cells experience both shear flow and compression [ 16 ]. (biomedcentral.com)
Moreover, the expression of C1QBP and XDH was lower in RCC tumors compared with the tumor-associated normal tissues, and their down-regulation was associated with higher Fuhrman grade. (medsci.org)

Diet-derived lipids may influence cytokine-mediated endothelial cell dysfunction, including TNF-induced apoptosis. (uky.edu)
The present study suggests that changes in the cellular lipid environment may markedly influence local TNF-induced events in the vascular endothelium, including endothelial cell apoptosis. (uky.edu)
Effects of three-dimensional culturing on osteosarcoma cells grown in a fibrous matrix: analyses of cell morphology, cell cycle, and apoptosis. (reprocell.com)
C1QBP promotes the catabolism of hypoxanthine and elevates the apoptosis of RCC cells by modulating XDH-mediated ROS generation. (medsci.org)

ECM gel can used with epithelial cells, endothelial cells, muscle cells, nerve cells and tumor cells. (sigmaaldrich.com)
ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue. (thermofisher.com)
Differences in the biological effects of crocidolite asbestos and two glass fibres on epithelial lung cells. (cdc.gov)

Pulmonary arterial hypertension (PAH) is a progressive disease characterized by lung endothelial cell dysfunction and vascular remodeling. (surrey.ac.uk)

In a separate project, scientists from the University of Oxford cultured the human pancreatic cancer cell line PSN-1 over a period of seven days, in either static or perfused conditions, using the Alvetex perfusion plate . (reprocell.com)

These effects of diabetic plasma on enzyme activity and cell adhesion, mediated by PKCβ2-dependent phosphorylation of the core 2 GlcNAc-T protein, were found to be triggered by increased plasma levels of tumor necrosis factor (TNF)-α. (diabetesjournals.org)
Induced EndoMT in PAECs by inflammatory cytokines IL-1β, tumor necrosis factor α, and transforming growth factor β led to actin cytoskeleton reorganization and the development of a mesenchymal morphology. (surrey.ac.uk)

During embryogenesis endothelial cells exhibit substantial plasticity that contribute to cardiac development by undergoing endothelial-to-mesenchymal transition (EndoMT). (surrey.ac.uk)

Here, we create multi-layered normal bladder assembloids and tumour assembloids by reconstituting tissue stem cell/tumour cell-derived organoids with multiple stromal components to represent an organized tissue architecture and functionality of adult bladder, and to mimic in vivo pathophysiological features of patient-derived urothelial carcinoma, respectively. (researchsquare.com)
TNT cellular networks are upregulated between cancer and stromal cells under hypoxic and other conditions of physiologic and metabolic stress. (frontiersin.org)
The field of investigation of TNT-mediated tumor-stromal, and tumor-tumor, cell-cell communication is gaining momentum. (frontiersin.org)

Over lifetime, due to the action of several stressors such as DNA damage and telomere shortening, senescent cells accumulate in the organism and release a variety of pro-inflammatory cytokines responsible for low-grade inflammation. (frontiersin.org)
Certain studies show that P. gingivalis lipopolysaccharide (LPS) could induce the expression of intercellular adhesion molecule 1 and vascular cell adhesion molecule 1 in human umbilical vein endothelial cells (HUVECs) ( 13 , 14 ), which significantly enhances trans-endothelial migration of inflammatory cells. (spandidos-publications.com)

Native honey (1% w/v pH 7.4, 10(6) cells) showed strong quenching activity against lipophilic cumoxyl and cumoperoxyl radicals, with significant suppression/prevention of cell damage, complete inhibition of cell membrane oxidation, of intracellular ROS production and recovery of intracellular GSH. (researchgate.net)

Altogether, by identifying a pro-angiogenic VEGF/miR-101/EZH2 axis in endothelial cells we provide evidence for a functional link between growth factor-mediated signaling, post-transcriptional silencing, and histone-methylation in the angiogenesis process. (plos.org)
Angiogenesis is dependent on the coordinated action of numerous cell types. (rupress.org)
Here, we show that although this receptor is present on most vascular and blood cells, the key regulatory function in tumor and wound angiogenesis is performed by β 3 integrin on bone marrow-derived cells (BMDCs) recruited to sites of neovascularization. (rupress.org)
Thus, β 3 integrin has the potential to control processes such as tumor growth and wound healing by regulating BMDC recruitment to sites undergoing pathological and adaptive angiogenesis. (rupress.org)

mRNA of ghrelin receptors(GHS-R1a and GHS-R1b), as well as, protein levels of GHS-R1a, were expressed abundantly in A2780 cells. (hindawi.com)

In fact, several studies have shown that T2D induces senescence in multiple types of cells, including fibroblasts and endothelial cells ( 11 , 12 ). (frontiersin.org)
Additionally, P. gingivalis induces macrophage foam cell formation ( 11 ) and stimulates oxidation of low-density lipoprotein ( 12 ). (spandidos-publications.com)
Studies have shown that xanthine dehydrogenase (XDH)-derived reactive oxygen species (ROS) accelerates tumor growth, and also induces mutations or produces cytotoxic effects concurrently. (medsci.org)

Binding of this protein to CXCR3 results in pleiotropic effects, including stimulation of monocytes, natural killer and T-cell migration, and modulation of adhesion molecule expression. (cancerindex.org)
Have 4 connected wells, allowing for greater volume provision for highly metabolic monocultures, or active solute flow for paracrine or cell migration co-cultures. (reprocell.com)

In breast cancers from patients, the density of TMEM doorways correlates with the proportion of cancer cells expressing stem cell markers, indicating that in human breast cancer TMEM doorways are not only cancer cell intravasation portals but also CSC programming sites. (nature.com)
In mammary gland development, Notch-dependent heterotypic signaling between resident tissue macrophages and mammary stem cells supports survival and function of the normal mammary stem cell 20 . (nature.com)

Organoids are derived from three major cell types: tissue-restricted adult stem cells, pluripotent stem cells and, in the case of tumour organoids, tumour cells (reviewed in 3,4 ). (researchsquare.com)

Such mechanisms may play a role in the damage and death of vascular endothelial cells in atherosclerosis. (uky.edu)
peptide 14 or 19 from P. gingivalis HSP60 may have either an anti- or pro-atherogenic role, respectively, in the ApoE(−/-) mouse model of infection-triggered atherosclerosis through distinct mechanisms operating in the polarization of T cells. (spandidos-publications.com)

6 months) bladder organoids, mouse embryonic fibroblasts (MEF), HULEC, bladder smooth muscle cells (BSMC) cultures in advance. (researchsquare.com)

We demonstrate that α v β 3 integrin enhances BMDC recruitment and retention at angiogenic sites by mediating cellular adhesion and transmigration of BMDCs through the endothelial monolayer but not their release from the bone niche. (rupress.org)
Because age-related cellular senescence and type 2 diabetes (T2D) have been recognised as risk factors for CRC development, the recent finding that type 2 diabetic patients present an elevated circulating volume of senescent cells raises the question whether type 2 diabetes facilitates the process of CRC tumorigenesis by inducing premature cell senescence. (frontiersin.org)

Together, these data indicate that matrix stiffening promotes the phosphorylation of Src, and that Src interacts with activated FAK to phosphorylate tyrosine residues on the cytoplasmic tail of VE-cadherin, which regulate endothelial barrier junction integrity via the mediation of catenin attachment. (cornell.edu)

We previously showed that VEGF signaling in primary endothelial cell cultures caused overexpression of VEGFR2 in a positive feed-forward loop, which is at least partly regulated by loss of miRNA-mediated control of VEGF receptor degradation. (plos.org)

spread of metastatic cells [3]. (researchgate.net)
CSCs have an enhanced ability to self-renew, which makes them uniquely capable of initiating and sustaining primary and metastatic tumor growth 3 . (nature.com)

however, many of their functional roles in mediating tumor growth remain unknown. (frontiersin.org)
It was observed that tumor cells strongly accelerate plasma coagulation as a result of: i) expression of the blood clotting initiator protein, a tissue factor, as shown by flow cytometry and functional assays (factor Xa formation in the presence of cells and factor VIIa), and ii) direct activation of prothrombin to thrombin by cells, as evidenced by hydrolysis of the synthetic substrate, S-2238, and the natural substrate, fibrinogen. (scielo.br)
Restoration of wild-type PTEN to glioblastoma cell lines lacking functional PTEN ablates hypoxia and IGF-1 induction of HIF-1-regulated genes. (ox.ac.uk)

Syncytin is expressed mainly in the human placenta and is implicated in placental syncytiotrophoblast cell fusion. (ecancer.org)
We consider the origin of syncytin, its normal role in human placentogenesis and data regarding its relevance in cell-cell fusion. (ecancer.org)
In the present study, we demonstrate that following exposure to plasma from diabetic patients, the human promonocytic cell line U937 exhibits a significant elevation in core 2 GlcNAc-T activity and increased adherence to cultured retinal capillary endothelial cells. (diabetesjournals.org)
We determine a comprehensive map of lineage-specific lncRNAs in human dermal lymphatic and blood vascular endothelial cells (LECs and BECs), combining RNA-Seq and CAGE-Seq. (nature.com)
Human A2780 ovarian cancer cells were cultured with or without AG treatment in the presence or absence of cisplatin. (hindawi.com)
In the present study, we dissected the molecular mechanisms responsible for the procoagulant activity of MV3, a highly aggressive human melanoma cell line. (scielo.br)

Rat vascular endothelial cells (RVECs) were patterned on collagen-coated nitrocellulose paper to create a tissue chip. (biomedcentral.com)

On the other hand, particular attention has been paid recently to the role of gut hormones in the tumorigenesis of various types of solid tumors. (hindawi.com)

Clear cell RCC (ccRCC) is the most common subtype among the malignant RCCs, accounting for 70-80%, followed by papillary (15%), chromophobe (4-5%), and other fewer subtypes[ 2 ]. (medsci.org)

During development, the blood vascular system arises from endothelial cell progenitors that differentiate from mesodermal cells, mostly through the expression of the transcription factor (TF) ETV2. (nature.com)
Histologic assessment of patients with systemic sclerosis-associated PAH and the hypoxia/SU5416 mouse model identified the presence von Willebrand factor/α-smooth muscle actin-positive endothelial cells in up to 5% of pulmonary vessels. (surrey.ac.uk)
And yet even with this advance, in addition to diffusible soluble factor-mediated paracrine and endocrine cell communication as well as EVs, additional niches of intratumoral communication are filled by other modes of intercellular transfer. (frontiersin.org)
In contrast, prothrombin activation was not observed when cells were previously incubated with DEGR-factor Xa, an inactive derivative of the enzyme. (scielo.br)
Moreover, a monoclonal antibody against bovine factor Xa reduced the prothrombin-converting activity of tumor cells. (scielo.br)
In conclusion, the data strongly suggest that MV3 cells recruit factor Xa from the culture medium, triggering an uncommon procoagulant mechanism. (scielo.br)

Thereafter, a distinct subpopulation of endothelial cells lining the cardinal vein starts differentiating by expressing the TF PROX1, the master regulator of lymphatic endothelial cell (LEC) identity, via the TFs SOX18 and COUPTFII. (nature.com)

Swedish Research Counsil (R.J.A.N.), National Cancer Institute (NCI) CA069246 and CA86355 (X.O.B.), and the American Brain Tumor Association (T.W.). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. (plos.org)
Cancer stem cells (CSCs) play an important role during metastasis, but the dynamic behavior and induction mechanisms of CSCs are not well understood. (nature.com)
Mechanistically, stemness is induced in non-stem cancer cells upon their direct contact with macrophages via Notch-Jagged signaling. (nature.com)
90% of cancer-related deaths, is a multi-step process involving cancer cell intravasation into the bloodstream, dissemination to distant sites, and seeding in new organs 1 . (nature.com)
At the apex of the hierarchy are the cancer stem cells (CSCs). (nature.com)
In this post, we will explore two examples of how perfusion systems can help improve the relevance of your 3D cell culture, based on two publications from Alvetex users in the field of developmental biology and cancer. (reprocell.com)

More recently, a number of studies have focused on the procoagulant mechanisms exhibited by tumor cells. (scielo.br)

Renal cell carcinoma is one of the most common cancers in the urinary system, causing over 140,000 deaths worldwide each year[ 1 ]. (medsci.org)

In conclusion, the findings of this study show that AG promotes cell survival of the OC cells and renders them resistat to Cis therapy, an effect that is mediated by the activation of PI3K/Akt/mTOR and activation of NF- κ B, and requires GHS-R1a. (hindawi.com)

In both control and Cis-treated cells, AG treatment significantly increased PI3K/Akt/mTOR signaling and enhanced the nuclear accumulation of NF- κ B. Concomitantly, in both control and Cis-treated cells, AG significantly lowered the protein levels of p53, p-p53 (Ser16), PUMA, cytochrome C, and cleaved caspase-3. (hindawi.com)
In glioblastoma-derived cell lines, PTEN does not significantly alter apoptotic sensitivity or cause complete inhibition of DNA synthesis. (ox.ac.uk)

The exact pathogenic mechanism by which capillary nonperfusion, as demonstrated by fluorescein angiography, occurs is still unclear, but recent experimental animal studies ( 3 - 5 ) demonstrate that increased leukocyte entrapment in retinal capillaries of diabetic rats is an early event associated with areas of capillary nonperfusion and the development of endothelial cell damage. (diabetesjournals.org)
1998. Activation of alveolar macrophages and peripheral red blood cells in rats exposed to fibers/particles. (cdc.gov)

PC12 cells will show neurite formation within 2 days when they are grown on a thin layer of this ECM gel. (sigmaaldrich.com)

To dissociate cells from this gel, use protease in PBS without calcium, magnesium, or EDTA, at a concentration of 0.6-2.4 units per mL. (sigmaaldrich.com)
The levels of vascular endothelial (VE)‑cadherin, endothelial nitric oxide synthase (eNOS) and cysteinyl aspartate‑specific protease‑3 (caspase‑3) were measured using western blot analysis. (spandidos-publications.com)

AG treatment did not affect the mRNA and protein levels of GHS-R1a and GHS-R1b in both control and Cis-treated cells. (hindawi.com)

Moreover, we found that glioblastoma cells, notorious for their VEGF production, elicited a similar response when co-cultured with endothelial cells [11] . (plos.org)

Could perfusion reduce metabolic stress in your cultures and improve the functionality of your cells? (reprocell.com)

A large body of evidence now implicates increased leukocyte-endothelial cell adhesion as a key early event in the development of diabetic retinopathy. (diabetesjournals.org)
We recently reported that raised activity of the glycosylating enzyme core 2 β 1,6- N -acetylglucosaminyltransferase (GlcNAc-T) through protein kinase C (PKC)β2-dependent phosphorylation plays a fundamental role in increased leukocyte-endothelial cell adhesion and capillary occlusion in retinopathy. (diabetesjournals.org)
A key adhesion molecule expressed by these cells is the α v β 3 integrin. (rupress.org)

For short-term exposure, endothelial cells were exposed to 18:2 for 6 h or to TNF for 1.5 h. (uky.edu)
For long-term exposure, endothelial cultures were treated with 18:2 for 24 h and with TNF for 19.5 h. (uky.edu)
In cells exposed to 18:2 + TNF, pretreatment with 18:2 began 4.5 h before additional exposure to TNF for either 1.5 h (short-term exposure) or 19.5 h (long-term exposure). (uky.edu)

J Cell Biol (2008) 183 (6): 1145-1157. (rupress.org)
Am J Respir Cell Mol Biol 10:253-258. (cdc.gov)

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