• On the other hand, grooves that are too wide can decrease the effects of contact guidance Cells can orient in response to contact guidance when located inside three-dimensional structures, such as collagen gels, scaffolds, and soft tissues. (wikipedia.org)
  • For example, it has been observed that endothelial colony forming cells align along the direction of the fibers present in electrospun scaffolds. (wikipedia.org)
  • On this study, the angiogenic functionality of human endothelial cells was studied after being plated on the ground of polyurethane-poly caprolactone (PU/PCL) scaffolds for 72 hours. (ncbcs.org)
  • These biomembranes or scaffolds constitute a support for osteoblastic adhesion and proliferation, but also microenvironment for stem cells' chemotactism and differentiation [ 1 ]. (hindawi.com)
  • My part of the research team is to create dispersion of poly-vinyl alcohol and cellulose to synthesize porous electrospun nanofibrous scaffolds, which will then be used in a 3D bioprinting application to further create 3D vasculature. (fairfield.edu)
  • Her research interests include the preparation and design of materials and scaffolds for in vitro and in vivo fundamental studies, and a further focus is the provision of useful tools to assess mechanisms that govern cell behavior in regenerative medicine. (ibecbarcelona.eu)
  • BC-Ppy scaffolds improved cell viability andattachment, promoting a desirable cardiomyoblast morphology. (ibecbarcelona.eu)
  • Specifically, the employment of BC-Ppy compositesdrives partial H9c2 differentiation toward a cardiomyocyte-like phenotype.The scaffolds increase the expression of functional cardiac markersin H9c2 cells, indicative of a higher differentiation efficiency,which is not observed with plain BC. (ibecbarcelona.eu)
  • Ghasemi-Mobarakeh L, Prabhakaran MP, Tian L, Shamirzaei-Jeshvaghani E, Dehghani L, Ramakrishna S. Structural properties of scaffolds: Crucial parameters towards stem cells differentiation. (wjgnet.com)
  • Scaffolds play an important role in tissue engineering as a substrate that can mimic the native extracellular matrix and the properties of scaffolds have been shown to affect the cell behavior such as the cell attachment, proliferation and differentiation. (wjgnet.com)
  • Electrospun polyurethane/poly (ɛ-caprolactone) nanofibers promoted the attachment and progress of human endothelial cells in static and dynamic tradition situations On this examine, the angiogenic capability of human endothelial cells was studied after being plated on the floor of polyurethane-poly caprolactone (PU/PCL) scaffolds for 72 hours. (wildpalm.net)
  • These scaffolds were then used to detect the response of mouse bone marrow stromal cells after implantation of the scaffold. (nanofiberlabs.com)
  • These nanofibers were made into three-layer tubular scaffolds by continuous electrospinning, and cultured endothelial cells and smooth muscle cells for biological activity evaluation. (nanofiberlabs.com)
  • Contact guidance refers to a phenomenon for which the orientation of cells and stress fibers is influenced by geometrical patterns such as nano/microgrooves on substrates, or collagen fibers in gels and soft tissues. (wikipedia.org)
  • In those conditions, the geometrical cues provided by collagen or scaffold fibers are able to influence the orientation of cells. (wikipedia.org)
  • Similarly, the collagen fibers present in collagen gels and soft tissues can influence cell alignment, providing the most important stimulus in terms of cell orientation Recent research has highlighted the importance of cellular alignment for the mechanical properties and functionality of the prostheses developed using the principles of tissue engineering. (wikipedia.org)
  • Recently, a computational model has been developed that is able to simulate the re-alignment of cells and stress fibers on top of grooved surfaces. (wikipedia.org)
  • In that case, the stress fiber assembly is isotropic, and these fibers can pull on their surroundings in an isotropic fashion, and consequently the resulting cell shape is isotropic (without a preferred alignment). (wikipedia.org)
  • Due to the anisotropic cellular contraction, stress fibers and cells align along the direction of the microgrooves. (wikipedia.org)
  • The biological activity of the surface-induced FG network to provide 3D cues in a potential tissue engineered scaffold, making use of electrospun PEA fibers (-OH(0)), seeded with human umbilical vein endothelial cells was investigated. (ibecbarcelona.eu)
  • The FG assembled on the polymer fibers gave rise to a biologically active network able to direct cell orientation along the fibers (random or aligned), promote cytoskeleton organization and focal adhesion formation. (ibecbarcelona.eu)
  • Interactions between endothelial cells and electrospun methacrylic terpolymer fibers for engineered vascular replacements. (ncsu.edu)
  • Investigated the role of ECM like fiber diameter on epithelial cluster formation and HGF induced scattering behavior via electrospun polycaprolactone (PCL) fibers conjugated with thiolated RGD as a biomimetic signal. (cecon.com)
  • Actin Stress Fibers stained with Acti-stain™ 555 in a Swiss 3T3 cell. (cytoskeleton.com)
  • Both mats supported cell adhesion, proliferation, cellular phenotype and spreading. (unipd.it)
  • The various areas that Shu Wang examines in his Electrospinning study include Cell morphology and Cell adhesion. (research.com)
  • His research in Cancer research intersects with topics in Immune checkpoint, Epithelium, In vitro, Cell therapy and Epithelial cell adhesion molecule. (research.com)
  • The final evaluation experiment results showed that the collagen in the middle layer significantly improved the adhesion and proliferation of SMC, and the proliferation of endothelial cells increased significantly with culture, indicating the non cytotoxicity of the construct. (nanofiberlabs.com)
  • In dermal fibroblasts culture in vitro, the mixed scaffold induced higher cell seeding efficiency and improved fibroblast adhesion and proliferation compared with collagen sponge. (nanofiberlabs.com)
  • Govindarajan T, Shandas R. Microgrooves Encourage Endothelial Cell Adhesion and Organization on Shape-Memory Polymer Surfaces. (ucdenver.edu)
  • Quantified role of soluble fibrin in receptor mediated tumor cell adhesion to pulmonary endothelial cells. (cecon.com)
  • air effective roles in cell adhesion, migration, in vivo angiogenesis and tumor advancement 35, 36. (upb.ro)
  • Endothelial cell attachment to an intravascular stent is promoted by coating the stent with an endothelial cell specific adhesion peptide. (patsnap.com)
  • Bone regeneration using electrospun nanofibers' implant has the following objectives: bone neoformation induction with rapid healing, reduced postoperative complications, and improvement of bone tissue quality. (hindawi.com)
  • Thus, the strategy of functionalization of nanofibers by nanoreservoirs of BMP-2 or BMP-7 showed a great efficiency for bone regeneration and increased the differentiation of MSCs (mesenchymal stem cells), accelerating the tissue regeneration in vivo [ 9 , 10 , 13 , 14 ]. (hindawi.com)
  • The results showed that compared with pure PLLA nanofibers, the mineral growth, ALP activity and cell morphology of the modified structure were in the best state. (nanofiberlabs.com)
  • A similar behavior has been observed with other cell types, such as fibroblasts, which align along these topographical patterns when the grooves are wider than 150 nm. (wikipedia.org)
  • Growth behavior of endothelial cells according to electrospun poly(D,L-lactic-co-glycolic acid) fiber diameter as a tissue engineering scaffold. (axogeninc.eu)
  • The physical properties of electrospun nanofibrous mats have a significant effect on the behavior of electrospun mat in interaction with other surfaces, as well as on its efficiency in various applications. (ac.ir)
  • The in vitro studies indicated that NIC-loaded BLVG could significantly increase the proliferation, migration and antioxidation capability of endothelial cells (ECs). (zju.edu.cn)
  • The results show that the P(LLA-CL)/fibrinogen nanofibrous membrane has a good cellular compatibility, and the high level porosity promotes the cell proliferation. (fzxb.org.cn)
  • Conditioned medium from these M2-like macrophages presented lower levels of proinflammatory cytokines and higher levels of proangiogenic factors and promoted endothelial cell proliferation, migration, and tube formation. (biomedcentral.com)
  • The final results showed that the cell proliferation in the double-layer scaffold was higher than that in the single-layer CP nano pad and PLLA microdisk. (nanofiberlabs.com)
  • This work focuses on the evaluation of the potential use of electrospun poly(epsilon-caprolactone) (PCL) micrometric and/or sub-micrometric fibrous membranes for rat hippocampal astrocyte (HA) and rat cerebro-microvascular endothelial cell (CEC) cultures. (unipd.it)
  • Functionalization of the surface of electrospun poly(epsilon-caprolactone) mats using zwitterionic poly(carboxybetaine methacrylate) and cell-specific peptide for endothelial progenitor cells capture, Q. Li et al. (baseclick.eu)
  • We have recently demonstrate the potential of electrospun poly(lactic acid) (PLA) fiber-based membranes, containing calcium phosphate (CaP) ormoglass particles, to elicit angiogenesis in vivo , in a subcutaneous model in mice. (diapathlabtalks.com)
  • In vitro experiments withcardiac fibroblasts and H9c2 cells confirmed the exceptional biocompatibilityof BC-Ppy composites. (ibecbarcelona.eu)
  • Coupled with ethical concerns around animal testing, this leads to high demand for improved in vitro cell culture platforms. (dcu.ie)
  • The latest development in in vitro cell culture models, organ-on-chips (OOCs), seeks to introduce more realistic models of organ function. (dcu.ie)
  • Biological studies confirmed the formation of a stable in vitro alveolar model assessed by the formation of confluent layers of human lung carcinoma epithelial cells (A549) and human pulmonary microvascular endothelial cells (hPMEC) on the opposite sides of the selected membrane as relevant cells to those of the alveolar-capillary barrier in the lung. (dcu.ie)
  • Fig. 3 Small Aperture (125-250 μ m) In vitro cartilage differentiation of human bone marrow mesenchymal stem cells induced by β - lactamase scaffold Large specific aperture (425-600 μ m) The stents in our body better support cartilage formation. (nanofiberlabs.com)
  • Synthesize novel hyaluronic acid hydrogels for in vitro culture of secretory epithelial cells. (cecon.com)
  • Using in vitro coagulation assay determined the cellmembrane bound tissue factor and melanoma cell invasiveness. (cecon.com)
  • Bilayer nicorandil-loaded small-diameter vascular grafts improve endothelial cell function via PI3K/AKT/eNOS pathway[J]. Journal of Zhejiang University Science D, 2021, 4(1): 72-86. (zju.edu.cn)
  • Data on biodistribution and radiation absorbed dose profile of a novel 64Cu-labeled high affinity cell-specific peptide for positron emission tomography imaging of tumor vasculature. (ncsu.edu)
  • Selective endothelial cell attachment to peptide-modified terpolymers. (ncsu.edu)
  • Selection and initial characterization of novel peptide ligands that bind specifically to human blood outgrowth endothelial cells. (ncsu.edu)
  • Next, we used a peptide array method that involved human dental pulp stem cells (hDPSCs) to evaluate the mineralization-inducing ability of each peptide. (nagoya-u.ac.jp)
  • An extensive body of work has focused on the use of angiogenic factors or endothelial progenitor cells. (diapathlabtalks.com)
  • Hydrogel network was tuned altering disulphide and ester linkages to allow cell assembly into spheroids. (cecon.com)
  • The electrospun randomized nanofiber network and the created micropores (diameter 100 μ m) mimic the pattern of the connective tissue matrix [ 4 , 5 ]. (hindawi.com)
  • A series of P(LLA-CL)/fibrinogen composite nanofiber membranes were prepared, and their micromorphology, surface composition, wettability, mechanical properties and cellular compatibility were characterized and analyzed by scanning electron microscope, Fourier-transformed infrared spectroscopy, X-ray photoelectron spectroscopy, and cell experiments. (fzxb.org.cn)
  • As a part of the same scientific family, Shu Wang mostly works in the field of Neurite, focusing on Nano- and, on occasion, Nanofiber, Elongation and Cell culture. (research.com)
  • Approaches of bioengineering and regenerative medicine aim to create different types of materials, implants, or scaffold mimicking structure of extracellular matrix, functionalized with bioactive molecules or living cells. (hindawi.com)
  • Here he also utilizes mesenchymal stem cells or their products incorporated in a biodegradable scaffold that undergoes substantial in vivo remodeling to develop a native-like blood vessel. (pitt.edu)
  • In actual preparation, electrospun PLLA scaffold will be prepared in combination with surface modification to guide cells to differentiate into bone lineage and achieve the best bone regeneration performance. (nanofiberlabs.com)
  • Different sources of living cells are described, as mesenchymal stem cells (MSCs), adipose-tissue derived stem cells, skin derived multipotent stem cells, or oral cavity MSCs, presenting compatible immunophenotype or morphology [ 2 , 3 ]. (hindawi.com)
  • He is developing a treatment strategy for abdominal aortic aneurysms by delivering mesenchymal stem cells or their secreted products to the periadventitial side of the aneurysm to inhibit the matrix degradation commonly seen in the disease progression and promote its regeneration. (pitt.edu)
  • His biological study spans a wide range of topics, including T cell, Immunology, Induced pluripotent stem cell, Mesenchymal stem cell and Immunotherapy. (research.com)
  • Fabrication of electrospun polymer fiber mats. (cecon.com)
  • This finding was correlated to the presence of larger voids within electrospun PCL microfibrous mats, suggesting that the morphology should be accurately selected for the realization of a cell environment-mimicking mat. (unipd.it)
  • During the Expo, engineering students Alana Hayes '24 and Ryan Jaworski '25 and biology students Elia Haghbin '26, Eliza Hogan '24, Grace Lombardi '25, and Elizabeth Ricci '23 presented phase one of their original research, "Developing a Human Blood Vessel Using 3D Bioprinting and Cell Culturing Techniques. (fairfield.edu)
  • This research is ultimately trying to cultivate human umbilical endothelial cells and human aortic smooth muscle cells, to be used in a 3D bioprinter to print a human vein with live cells," said Jaworski. (fairfield.edu)
  • Human T cells signify a heterogeneous inhabitants, together with cells with completely different phenotypical and performance properties. (heraeus-targets.com)
  • We previously demonstrated expression and activity of the subtype hTAS2R46 in human airway smooth muscle and broncho-epithelial cells, and here we show its expression and functionality in human skeletal muscle cells. (bvsalud.org)
  • Three different cellular models were used: micro-dissected human skeletal tissues, human myoblasts/myotubes and human skeletal muscle cells differentiated from urine stem cells of healthy donors. (bvsalud.org)
  • We show, for the first time, experimentally the presence and functionality of a type 2 bitter receptor in human skeletal muscle cells. (bvsalud.org)
  • Human primary salivary gland acinar like cells were assembled into tightly adhered spheroid structures using a multi well non adherent cell culture system. (cecon.com)
  • that researchers drew increasing attention on this topic, seeing the potential of contact guidance in influencing the morphology and organization of cells. (wikipedia.org)
  • Two-dimensional (2D) tradition of cells from big cell tumor of bone (GCTB) is affected by lack of the multinucleated big cells in subsequent passages. (ncbcs.org)
  • Synthesis and comparative evaluation of novel Cu-64-labeled high affinity cell-specific peptides for positron emission tomography imaging of tumor vasculature. (ncsu.edu)
  • The vascular endothelial progress issue (VEGF)/VEGF receptor (VEGFR) axis is an important regulator of angiogenesis and vital therapeutic goal in most cancers. (ncbcs.org)
  • Can the vascular endothelial growth factor (VEGF) and total estradiol 17β be used as a marker of equine oocyte maturation? (cloud-clone.com)
  • Once formed, the focal adhesions produce a signal that starts to diffuse into the cell inducing stress fiber assembly. (wikipedia.org)
  • His study in Induced pluripotent stem cell is interdisciplinary in nature, drawing from both Regenerative medicine, Antigen, Genetic enhancement, Cell biology and Neural stem cell. (research.com)
  • Current OOCs often use commercial porous polymeric membranes as a barrier membrane for cell culture which is challenging due to the poor replication of the physiological architectures. (dcu.ie)
  • The electrospinning process was first optimised regarding the solvent selection (theoretically & experimentally) and the processing parameters (using Response Surface Methodology) to achieve electrospun membranes with properties correlating to those of the native BM. (dcu.ie)
  • Based on this, cells seem to be able to sense the structural characteristics of their surrounding and consequently respond by adopting the orientation of topographical stimuli. (wikipedia.org)
  • Stem cells have attracted much interest in tissue engineering as a cell source due to their ability to proliferate in an undifferentiated state for prolonged time and capability of differentiating to different cell types after induction. (wjgnet.com)
  • The many advances made in cell culture, biomaterials, manufacturing techniques, and tissue engineering methods have led to various promising solutions for vascular graft production, with available options able to recapitulate both biological and mechanical properties of native blood vessels. (bvsalud.org)
  • Using parallel plate flow chamber, build a kinetic relationship for soluble fibrin mediated melanoma cell capture mechanism to pulmonary endothelium via the involvement of polymorphonuclear neutrophils. (cecon.com)
  • Focused therapies and immune checkpoint inhibitors have superior the therapy panorama of Renal Cell Carcinoma (RCC) over the past decade. (cellexusinc.com)
  • The main interest of the use of bone marrow derived stem cells is their osteogenic potential for neoangiogenesis. (hindawi.com)
  • His Cell biology research incorporates themes from Immunology, Embryonic stem cell, Biochemistry and Neuron. (research.com)
  • His Cancer research study integrates concerns from other disciplines, such as Cytotoxic T cell, Chimeric antigen receptor, Induced pluripotent stem cell and Suicide gene. (research.com)
  • Generation of 'Off-the-Shelf' Natural Killer Cells from Peripheral Blood Cell-Derived Induced Pluripotent Stem Cells. (research.com)
  • M2 macrophages and exosomes from adipose-derived stem cells (ASCs) are both reported to promote angiogenesis. (biomedcentral.com)
  • Laser scanning confocal microscopy is a significant advance in the field of optical microscopy, primarily because it permits sample visualization deeper within living and fixed cells, tissues and other samples. (sheffield.ac.uk)
  • Immunohistochemistry (IHC) and immunocytochemistry (ICC) are extensively used to determine cancerous cells inside tissues and cell cultures. (ncbcs.org)
  • However, substrates with topographical patterns influence the orientation of cells cultured on these surfaces by their geometrical cues. (wikipedia.org)
  • In order to explore receptor activity, cells were incubated with the specific bitter ligands absinthin and 3ß-hydroxydihydrocostunolide, and calcium oscillation and relaxation were evaluated by calcium imaging and collagen assay, respectively, after a cholinergic stimulus. (bvsalud.org)
  • Shu Wang focuses on Cancer research, Chimeric antigen receptor, NKG2D, Cytotoxic T cell and Immune system. (research.com)
  • His primary scientific interests are in Cytotoxic T cell, Cancer research, Immunology, Chimeric antigen receptor and Interleukin 12. (research.com)
  • Exclusion criteria in return the exploration were disorders that may affect endothelial function, such as cardiovascular contagion (hypertension, diabetes mellitus, peripheral artery malady, heart failure) and cardiovascular levelts in the past (stroke, transient ischemic attack (TIA), myocardial infarction). (upb.ro)
  • For example, if a substrate has nano/microgrooves running parallel to each other, cells orient along the direction of these nano/microgrooves. (wikipedia.org)
  • Current research have drawn consideration to the nonanesthetic results of propofol, however the potential mechanism by which propofol suppresses non-small-cell lung most cancers (NSCLC) development has not been totally elucidated. (cellexusinc.com)
  • Apoptosis, or programmed cell death, is a highly regulated process used by many multicellular organisms. (apoptosis-inhibitors.com)
  • Blastic plasmacytoid dendritic cell neoplasm (BPDCN) is an especially uncommon tumour, which often impacts aged males and presents within the pores and skin with frequent involvement of the bone-marrow, peripheral blood and lymph nodes. (ncbcs.org)