Endothelial cells actin cell cycle. Medical search. Frequent questions

They are involved in lysine degradation, adherens junction, the TGF-ß signaling pathway, the p53 signaling pathway, the Hippo signaling pathway, the forkhead box O (FoxO) signaling pathway, regulation of actin cytoskeleton, and RNA degradation based on Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. (bvsalud.org)
This result suggests that phospho-ezrin is important for actin cytoskeleton regulation during erythroid cell growth and division. (biomedcentral.com)
The important role of SMC in stabilizing the cytoskeleton is highlighted in individuals with mutations in ACTA2 encoding for smooth muscle actin or its promoter, major to a higher threat for coronary illness (333). (adenosine-kinase.com)
Moreover, MCT4 overexpression enhances cell migration and invasiveness via reorganization of the actin cytoskeleton. (hindawi.com)
Upon the formation of this molecular complex, endorepellin triggers rapid dissolution of the actin cytoskeleton, VEGFA suppression, and induction of autophagy. (jefferson.edu)
Actin staining is very useful in determining the structure and function of the cytoskeleton in living a nd fixed cells. (cytoskeleton.com)
The actin cytoskeleton is a very dynamic and labile structure in the living cell, but it can be fixed with paraformaldehyde prior to probing or staining for actin structures. (cytoskeleton.com)

During angiogenesis, actin-rich filopodia protrusions have been proposed to drive endothelial tip cell functions by translating guidance cues into directional migration and mediating new contacts during anastomosis. (biologists.com)
To investigate the structural organisation, dynamics and functional importance of F-actin in endothelial cells (ECs) during angiogenesis in vivo , we generated a transgenic zebrafish line expressing Lifeact-EGFP in ECs. (biologists.com)
By reversing cell cycle blockage, promoting wound healing ability, improving colony-forming capacity, and increasing angiogenesis in H2O2-treated cells, UA exhibited positive effects on cardiomyocyte growth characteristics. (bvsalud.org)
While acknowledging progress in the other areas mentioned, this work will focus on the current debates concerning sourcing, MSC alterations of angiogenesis, cell differentiation/stimulation, and strategies to improve MSC differentiation. (biomedcentral.com)
For this purpose, we designed an MCT4 overexpression and knockdown/inhibition model to explore the functional consequences of MCT4 expression on cancer cell metabolism, survival, proliferation, migration, invasion potential, and angiogenesis as well as on cell cycle profiles and cell death mechanisms. (hindawi.com)
IL-1 stimulates the proliferation of inflammatory cells and promotes angiogenesis through endothelial cell replication. (medscape.com)
microRNAs have emerged as master regulators of stem cell lineage differentiation and angiogenesis [ 14 ]. (biomedcentral.com)
Altogether, by identifying a pro-angiogenic VEGF/miR-101/EZH2 axis in endothelial cells we provide evidence for a functional link between growth factor-mediated signaling, post-transcriptional silencing, and histone-methylation in the angiogenesis process. (plos.org)
An increasing number of studies have demonstrated that EPCs are of great value in regenerative medicine and clinical applications, which can not only directly participate in angiogenesis but also participate in vascularization and endothelial repair by differentiating into endothelial cells [ 7 ]. (biomedcentral.com)
i.p) or vehicle administered 24 hours after permanent middle cerebral artery occlusion (pMCAO) on behavior, angiogenesis, ultra-structural integrity of brain capillary endothelial cells, and expression of EPO and VEGF were assessed. (researchgate.net)
These results suggested that catalpol might contribute to infarcted-brain angiogenesis and ameliorate the edema of brain capillary endothelial cells (BCECs) by upregulating VEGF and EPO coordinately. (researchgate.net)
The cyclic nature of the capillary bed in the corpus luteum offers a unique experimental model to examine the life cycle of endothelial cells, involving discrete physiologically regulated steps of angiogenesis, blood vessel maturation and blood vessel regression. (biomedcentral.com)
Available evidence indicates that microvascular endothelial cells of the corpus luteum are not alike, and may differ during the process of angiogenesis and angioregression. (biomedcentral.com)
This antibody panel comprises the critical markers for angiogenesis research, including endothelial marker CD34 antibody, basement membrane marker Collagen IV antibody, and mural cell markers NG2 and alpha-SMA antibodies. (arigobio.cn)

The aim of this study is to investigate the role of activated ovarian endothelial cells in early in-vitro follicular development. (biomedcentral.com)
This model is a novel platform for the in vitro maturation of early follicles and for the future exploration of endothelial-follicular communication. (biomedcentral.com)
Moreover, in vitro cultures of erythroid cells derived from haematopoietic stem cells has demonstrated that P. vivax is able to directly inhibit erythroid cell proliferation and differentiation [ 22 ]. (biomedcentral.com)
Distinct hallmarks of cancer were examined using in silico analysis, various in vitro cell culture assays, and ex vivo organotypic rat brain slice culture model. (hindawi.com)
We investigated the impact on tumor malignancy in F98 glioma cells using in silico analysis, in vitro cell culture assays, and ex vivo vascular organotypic glioma impact model (VOGIM) [ 35 ] by implanting F98 cells into rat brain slices. (hindawi.com)
Hydrogen peroxide was used to induce an oxidative stress model of hepatocyte IAR-20 cells to evaluate the protective effects of BMMSCs in vitro. (medscimonit.com)
In vitro , the sprouting and migratory properties of primary endothelial cell cultures were reduced by inhibiting EZH2 through up-regulation of miR-101, siRNA-mediated knockdown of EZH2, or treatment with 3-Deazaneplanocin-A (DZNep), a small molecule inhibitor of EZH2 methyltransferase activity. (plos.org)
Decades of efforts have attempted to differentiate the pluripotent stem cells (PSCs) into truly functional hematopoietic stem cells (HSCs), yet the problems of low differentiation efficiency in vitro and poor hematopoiesis reconstitution in vivo still exist, mainly attributing to the lack of solid, reproduced, or pursued differentiation system. (biomedcentral.com)
In this study, we established an in vitro differentiation system yielding in vivo hematopoietic reconstitution hematopoietic cells from mouse PSCs through a 3D induction system followed by coculture with OP9 stromal cells. (biomedcentral.com)
Flow cytometry analysis, RNA-seq, and cell cycle analysis were used to detect the in vitro hematopoietic ability of endothelial protein C receptor (EPCR, CD201) cells generated in our induction system. (biomedcentral.com)
Further, we innovatively demonstrated that notch signaling pathway is responsible for in vitro CD201 + hematopoietic cell induction from mouse PSCs. (biomedcentral.com)
Important insights on the morphology and function of luteal endothelial cells have been gained from a combination of in vitro and in vivo studies on endothelial cells. (biomedcentral.com)
Fetal equine dermal (Given) cells are vunerable to EIAV in vitro, as well as the replication of EIAV in Given cells transiently transfected with ELR1-IN was markedly decreased in comparison to replication in cells transfected using the unfilled vector. (angiogenesis-blog.com)
Preparation of stabilized fluorescent actin filaments in vitro. (cytoskeleton.com)

On the other end of the spectrum, the microvascular endothelial cells are the first cells to undergo apoptosis at the onset of corpus luteum regression. (biomedcentral.com)
The review highlights areas for future investigation of ovarian microvascular endothelial cells. (biomedcentral.com)

Depletion of STK35L1 by siRNA impaired endothelial cell migration. (wikipedia.org)
STK35L1 may act as a central kinase linking the cell cycle and migration of endothelial cells. (wikipedia.org)
Actin filaments are instrumental in driving processes such as migration, cytokinesis and endocytosis and provide cells with mechanical support. (biologists.com)
The formation of new vessels in the hypoxic area restores blood flow, provides oxygen and nutriments to the surviving cells, and promotes the migration and engraftment of new cells. (elifesciences.org)
Knocking down miR-31 levels in CB-EPCs suppressed cell migration and microtubule formation, while overexpressing miR-31 in PB-EPCs helped to recapitulate some of CB-EPC functions. (biomedcentral.com)
Atherosclerosis occurs earlier and with greater severity in the population with diabetes [ 1 - 3 ] and is characterized pathologically by endothelial cell injury, proliferation and migration of vascular smooth muscle cells (VSMCs), and thickening of intima [ 4 , 5 ]. (hindawi.com)
The encoded protein is expressed predominantly in endothelial cells of capillaries as well as larger vessels of the placenta where it may mediate the inhibitory effect of angiostatin on tube formation and the migration of endothelial cells toward growth factors during the formation of new blood vessels. (bioactiva.com)
Also, our mutants induced proliferation, glucose uptake, inhibited the migration of 293T cells and affected the responsiveness of the cells to cisplatin and radiation. (scielo.br)

Vascular endothelial cells, as a monolayer in direct contact with the flowing blood, bear the most of the wall shear stresses and have important homeostatic functions in response to stress [16] . (plos.org)
Separated in the media by the EGF Proteins supplier internal elastic membrane, the intima consists of loose connective tissue intermingled with few SMCs, that is covered by a monolayer of endothelial cells resting on a basal membrane. (adenosine-kinase.com)
Furthermore, monocyte transmigration through the endothelial monolayer was significantly increased by oxLDL treatment under HG conditions. (spandidos-publications.com)
Subsequently, rosmarinic acid treatment significantly reversed oxLDL‑induced overexpression of adhesion molecules and monocyte‑EC adhesion, oxLDL‑induced endothelial junction hyperpermeability and monocyte transmigration through the endothelial monolayer under HG conditions, in a dose‑dependent manner. (spandidos-publications.com)
Endothelial cells (ECs) form a semi-permeable monolayer that separates the bloodstream from underlying tissues and regulate the infiltration of blood cells and proteins through the vessel wall ( 1 , 2 ). (spandidos-publications.com)
Permeability was assessed by the efflux of fluorescein isothiocyanate (FITC)-dextran permeated through the monolayer endothelial cells (ECs). (springer.com)

Application of unidirectional pulsatile shear stress to human umbilical vein endothelial cells (HUVECs) decreased PDCD4 protein but not mRNA level. (plos.org)
The cell viability of human umbilical vein endothelial cells (HUVEC) in high doses of these two contrast agents were measured using the xCELLigence Real-Time Cell Analysis (RTCA) S16 Instrument. (dovepress.com)
However, the effects of NAC on LPS‑induced apoptosis of human umbilical vein endothelial cells (HUVECs) and the associated mechanisms are not well characterized. (spandidos-publications.com)
Thus, umbilical cord blood EPCs hold great therapeutic potential for cell therapy and vascular engineering. (biomedcentral.com)
EPCs from human umbilical cord and adult peripheral blood activate different mechanisms upon high-dose x-ray radiation treatment: CB-EPCs undergo p53 stabilization, Bax-dependent apoptosis and p21-dependent G 1 and G 2 /M cell cycle checkpoints, while PB-EPCs undergo only radiation-induced senescence [ 13 ], indicating unique gene expression patterns in EPCs of different sources. (biomedcentral.com)

Tissue samples (spleen, lung, intestine) were analyzed by using 2 real-time reverse transcriptase-quantitative PCR techniques targeting BTV segment 5 and host β-actin mRNA as a control. (cdc.gov)
Although the internal control was originally designed to detect β-actin mRNA of bovine or ovine species, clear positive signals were noted in all lynx samples, which indicated that this was a reliable control procedure. (cdc.gov)
Compared to cells in normal glucose, high glucose increased H3K9me3 and Suv39H1 binding at Rac1 promoter, and Suv39H1 -siRNA prevented glucose-induced increase 5 hydroxy methyl cytosine (5hmC) and Rac1 mRNA. (nature.com)
This customer written article "Use of Immunomagnetic Separation, mRNA Isolation and RT-PCR for Detection of Activated Human Monocytes in Whole Blood" was published in DYNALogue 2/99 and is a good example of the combined use of cell-specific IMS followed by mRNA isolation. (thermofisher.com)
To normalise the measurements in different samples due to variations in the isolation steps (IMS and mRNA), the expression of the housekeeping-gene, b-actin, was quantified (4). (thermofisher.com)
The b-actin-mRNA expression in the LPS treated samples was set to 100% and the other samples adjusted accordingly. (thermofisher.com)
TF-mRNA, TNFa-mRNA and b-actin-mRNA were analysed after pilot studies to determine the optimal number of PCR-cycles to be within the linear range of amplification for both the gene of interest and the housekeeping gene. (thermofisher.com)

VEGF, produced in large amounts by cancer cells during tumor growth, interacts with its receptors VEGFR1 and VEGFR2 thereby causing endothelial cell survival, proliferation, and sprouting [7] . (plos.org)
We previously showed that VEGF signaling in primary endothelial cell cultures caused overexpression of VEGFR2 in a positive feed-forward loop, which is at least partly regulated by loss of miRNA-mediated control of VEGF receptor degradation. (plos.org)
Moreover, we found that glioblastoma cells, notorious for their VEGF production, elicited a similar response when co-cultured with endothelial cells [11] . (plos.org)
Resveratrol also down-regulated the increased expressions of vascular endothelial growth factor (VEGF) and kinase insert domain receptor (KDR, or VEGF receptor-2) induced by high glucose. (springer.com)
On the other side, vascular endothelial growth factor (VEGF), one of the strongest hyperpermeability inducers (Senger et al. (springer.com)
In addition to binding to the insulin receptor, IRS1 also binds to and transmits signals from the receptors of prolactin, growth hormone (GH), leptin, vascular endothelial growth factor (VEGF), tropomyosin receptor kinase B (TrkB), anaplastic lymphoma kinase (ALK), insulin like growth factor (IGF1), and integrins ( Vuori and Ruoslathi , 1994 Vuori K and Ruoslahti E (1994) Association of insulin receptor substrate-1 with integrins. (scielo.br)

Cells were transfected with the early B-cell factor-2 (EBF2) to activate progenitor cell differentiation and gauged the tumorigenic potentials of the derived cell subsets. (amegroups.org)
Mechanistically, EBF2 uniformly activates Pparγ-expression in pancreatic cancer cells and limits cell proliferation, whereas oncogenic drivers are expressed differentially in the progenitor cell subsets. (amegroups.org)
This study reveals a previously unrecognized function of EBF2 in regulating PDAC progenitor cell differentiation and tumor suppression. (amegroups.org)

Apoptosis was measured by Hoechst 33258/propidium iodide double staining of nuclear chromatin and the formation of gaps into the lymphendothelial barrier in a three-dimensional co-culture model consisting of MCF-7 tumour cell spheroids and human lymphendothelial monolayers. (nature.com)
In HL-60 leukaemia cells, di-GA activated caspase 3 and dose-dependently induced apoptosis. (nature.com)
These data show that di-GA exhibits three distinct anticancer activities: induction of apoptosis, cell-cycle arrest and disruption of cancer cell-induced lymphendothelial disintegration. (nature.com)
Low PDCD4 level was associated with reduced proliferation but not apoptosis or phosphorylation of endothelial nitric oxide synthase caused by pulsatile shear stress to help maintain the homeostasis of endothelial cells. (plos.org)
Interestingly, endothelial expression of a degradation-resistant form of IB did not influence embryonic improvement, while endothelial cell-specific knockout of IKK resulted in enhanced embryonic lethality and endothelial apoptosis, which was at the least in part mediated by kinase-independent functions of IKK (326). (adenosine-kinase.com)
The results of CCK‑8 and flow cytometric analyses showed that pretreatment of HUVECs with NAC or p38MAPK inhibitor (SB203580) attenuated LPS‑induced decrease in cell viability and increase in cell apoptosis. (spandidos-publications.com)
However, few studies have investigated LPS-induced apoptosis of vascular endothelial cells. (spandidos-publications.com)
Apoptosis refers to the self-regulated and orderly death of cells controlled by genes in order to maintain the stability of internal environment ( 8 ). (spandidos-publications.com)
The apoptosis of endothelial cells is a key event that may impair the integrity of the vessel wall and lead to the formation of atherosclerotic plaques ( 9 ). (spandidos-publications.com)
The death receptor pathway plays an important role in cell apoptosis ( 12 ). (spandidos-publications.com)
A series of caspases proteins have been shown to be activated by the death induced signal complex [a form of death ligand that binds to the corresponding death receptor on the cell surface ( 13 )], which in turn induces apoptosis. (spandidos-publications.com)

Purpose: Human corneal endothelial cells (hCECs) have been considered unable to regenerate in vivo, resulting in corneal decompensation after significant loss of hCECs. (bvsalud.org)
The in vivo hematopoietic reconstitution potential of c-kit + cells derived from the mouse PSCs was evaluated via m-NSG transplantation assay. (biomedcentral.com)
The c-kit + cells from 3D self-assembling peptide induction system followed by the OP9 coculture system possessed apparently superiority in terms of in vivo repopulating activity than that of 3D induction system followed by the 0.1% gelatin culture. (biomedcentral.com)
Altogether, our findings lay a foundation for improving the efficiency of hematopoietic differentiation and generating in vivo functional HSC-like cells from mouse PSCs for clinical application. (biomedcentral.com)
In fact, only using the OP9 co-culture system combined with hematopoietic related cytokines, we hardly obtained the in vivo functional reconstitution hematopoietic cells from PSCs [ 9 ]. (biomedcentral.com)
Pre-incubation from the recombinant sELR1 with EIAV inhibited EIAV an infection in equine macrophages considerably, the principal in vivo focus on cell from the trojan. (angiogenesis-blog.com)

However, it can interact with PDLIM1/CLP-36 in the cytoplasm and localize to actin stress fibers. (wikipedia.org)
Actin Stress Fibers stained with Acti-stain™ 555 in a Swiss 3T3 cell. (cytoskeleton.com)

Previously, STK35L1 was named as Clik1 (CLP36 Interacting Kinase 1) based on a study that showed an association of STK35 with CLP36 after overexpression of both proteins in osteosarcoma cells. (wikipedia.org)
Endothelial cell lineage tracing showed that BNP directly stimulated the proliferation of resident endothelial cells via NPR-A binding and p38 MAP kinase activation. (elifesciences.org)
Many molecular pathways in eukaryotic cells are modulated by specific signaling proteins that are controlled, by phosphorylation and dephosphorylation, through the activity of kinase and phosphatase enzymes. (biomedcentral.com)
IRE1 RNase inhibition (MKC8866) and PERK kinase inhibition (GSK-2606414) affect autophagy flux in U251 and U87 cells (p62 degradation and LC3β-II/LC3β-I ratio) in different ways. (mdpi.com)
They've very low cell division rates, a very restricted migratory behavior and express high levels of contractile proteins, including myosin heavy chain, myosin light chain kinase, calponin, smooth muscle actin, and SM22. (adenosine-kinase.com)

The purpose of this study was to determine the acute and long-term effects of mitomycin C (MMC) on quiescent rabbit corneal keratocytes regarding cell proliferation, myofibroblast differentiation and DNA repair. (molvis.org)
MMC induces DNA damage to quiescent corneal keratocytes, which remains unrepaired, resulting in abnormal cell replication and gene transcription that leads to long-term effects on corneal repair. (molvis.org)
These data indicate that the effect of MMC on corneal scarring and haze is related to the generation of DNA ICLs leading to defective cell replication and gene expression. (molvis.org)

Ample evidence shows that pro-atherosclerotic disturbed flow induces sustained activation of atherogenic genes in endothelial cells to promote their oxidation, inflammation, cell cycle progression and proliferation, whereas pulsatile shear stress tends to maintain endothelial cells in a quiescent and less proliferative state with a low level of oxidation and inflammation [16] , [17] . (plos.org)
Endothelial inflammation increases the adhesiveness of monocytes to the endothelium in addition to increasing vascular permeability, promoting diabetic atherogenesis. (spandidos-publications.com)
microRNAs also play a crucial role in endothelial inflammation, senescence and susceptibility to atherosclerosis: endothelial inflammation is critically regulated by miRNAs such as miR-126 and miR-10a, and endothelial aging is additionally controlled by miR-217 and miR-34a [ 15 ]. (biomedcentral.com)

In this study, a phosphoproteomic approach was performed to dissect the molecular mechanism of phosphoprotein regulation, which is involved in the inhibitory effect of parasites on erythroid cell development. (biomedcentral.com)
These findings suggest that parasite proteins are able to inhibit erythroid cell growth by down-regulation of ezrin phosphorylation, leading to ineffective erythropoiesis ultimately resulting in severe malarial anaemia. (biomedcentral.com)
The regulation of complex and dynamic signal transduction proteins contributes to the destination of targeting proteins and the signal transduction of cell growth, and exposure to parasites can also influence signaling pathways. (biomedcentral.com)
Recent mechanistic insights into the trophic activities of these cells point to ultimate regulation by nitric oxide, nuclear factor-kB, and indoleamine, among other signaling pathways. (biomedcentral.com)
Our results further indicate that MCT4 promotes proliferation and survival by altered cell cycle regulation and cell death mechanisms. (hindawi.com)
Previous studies on GGTase-I mainly focused on the regulation of cell growth and differentiation via the Rac1 pathway and have been proved to be closely related to the occurrence, invasion, and metastasis of various malignant tumors [ 7 ]. (hindawi.com)
MMC significantly blocked TGFβ-induced cell proliferation and myofibroblast differentiation in cultured quiescent keratocytes and altered the transcriptional regulation of macrophage chemotactic protein-1 (MCP-1) and alpha smooth muscle actin (αSMA). (molvis.org)
The regulation of endothelial cell proliferation in the corpus luteum of pregnancy is less clear. (biomedcentral.com)

adipose-derived mesenchymal stem cell (ASC)-derived exosomes can regenerate tissues and organs. (bvsalud.org)
These elaborate signals promoted by ECs have been termed "angiocrine factors" and have been shown to be crucial for the maintenance of organ-specific tissues and tumor cells [ 12 ]. (biomedcentral.com)
In this study, we showed that Annexin A2 was evidently overexpressed in human NSCLCs cell lines and NSCLCs tissues. (jcancer.org)
The results displayed that LINC00659 is upregulated in IVC tissues and isolated endothelial group cells (EPCs) of patients with LEDVT. (biomedcentral.com)

One complementary therapy could be 're-activating' vasculogenesis (i.e. the differentiation of precursor cells into mature endothelial cells), a mechanism that occurs in the heart during development but is quiescent in adult hearts. (elifesciences.org)
In previously reports , Plasmodium vivax was found to be able to directly inhibited erythroid cell proliferation and differentiation. (biomedcentral.com)
MSCs can be defined by their ability to adhere to tissue culture plastic, their expression of several cell surface molecular epitopes-cluster of differentiation CD73, CD90, and CD105, and others-as well as their lack of several surface markers, including CD45 [ 6 ]. (biomedcentral.com)
We interestingly found that our 3D+OP9 system enriched a higher percentage of CD201 + c-kit + cells that showed more similar HSC-like features such as transcriptome level and CFU formation ability than CD201 - c-kit + cells, which have not been reported in the field of mouse PSCs hematopoietic differentiation. (biomedcentral.com)
Here we show that cell differentiation suppresses tumor formation. (amegroups.org)
Here we show that the brown adipose differentiation gene, EBF2, activates differentiation-associated signals in the cancer progenitor cells in culture and CSC transplantation models. (amegroups.org)

Protein activation and expression were analysed by western blotting, deoxyribonucleoside triphosphate levels by HPLC, ribonucleotide reductase activity by 14 C-cytidine incorporation into nascent DNA and cell-cycle distribution by FACS. (nature.com)
A combination treatment of Simvastatin (Simva) and Temozolomide (TMZ) inhibits autophagy flux and induces unfolded protein response (UPR) in GBM cells (U251, U87). (mdpi.com)
ECs were treated with oxLDL under HG conditions in the presence or absence of ROS scavengers mitoTEMPO and NAC, p38 inhibitor SB203580, FOXO1 inhibitor AS1842856 or transfected with the TXNIP siRNA, before protein expression levels of intercellular adhesion molecule 1 (ICAM‑1), vascular cell adhesion molecule‑1 (VCAM‑1), phosphorylated vascular endothelial‑cadherin (VE‑cadhedrin), VE‑cadherin and zonula occludens‑1 (ZO‑1) were measured by western blotting. (spandidos-publications.com)
In addition, oxLDL‑stimulated ECs under HG conditions exhibited increased phosphorylated VE‑cadherin protein levels and decreased ZO‑1 protein expression levels compared with those in untreated ECs, suggesting increased endothelial permeability. (spandidos-publications.com)
Tumor and calor develop as vascular endothelial gaps enlarge, allowing the egress of plasma protein and fluid into the interstitial space. (medscape.com)
BMMSCs also significantly improved the expression level of microtubule-associated protein 1 light chain 3 (LC3)-II in both DCD donor livers and ROS-induced injured IAR-20 cells, including upregulating the expression of ferritin. (medscimonit.com)

A essential part of endothelial NF-B signaling has also been shown in mouse models of atherosclerosis exactly where ablation of canonical NF-B signaling by endothelial cell-specific deletion of NEMO or overexpression of a dominant-negative variant of IB protected ApoE-deficient mice from atherosclerosis induced by a Western-type diet regime (327). (adenosine-kinase.com)
Generally, atherosclerosis is usually regarded as as chronic inflammatory illness with the vasculature, that is characterized by a complicated crosstalk among diverse cell types, with endothelial cells constituting a important beginning point of a vicious cycle, wherein NF-B activation will not only lead to the expression of adhesion molecules that bind leukocytes, but also causes secretion of inflammatory mediators, which activate smooth muscle cells. (adenosine-kinase.com)
This phenotypic shift was reported to become involved in endothelial dys Natural Killer Group 2, Member D (NKG2D) Proteins MedChemExpress function throughout atherosclerosis. (adenosine-kinase.com)
A number of studies have previously suggested that vascular endothelial dysfunction caused by increased junctional permeability is a key event in the development and progression of atherosclerosis and atherosclerotic cardiovascular diseases ( 3 , 4 ). (spandidos-publications.com)
The progressive impairment of endothelial function and integrity starts a cascade of events, leading to microcirculation damage, atherosclerosis and common cardiovascular disease (CVD), such as coronary heart disease (CHD), myocardial infarction (MI), heart failure, stroke and peripheral arterial disease (PAD) [ 1 ]. (biomedcentral.com)
The proliferation of vascular smooth muscle cells (VSMCs) induced by oxidative injury is one of the main features in diabetes-accelerated atherosclerosis. (hindawi.com)
Endothelial hyperpermeability induced by hyperglycemia is the initial step in the development of atherosclerosis, one of the most serious cardiovascular complications in diabetes. (springer.com)

Conclusions: ASC-derived exosomes promoted wound healing and regeneration of endothelial cells by inducing a shift in the cell cycle and suppressing senescence and autophagy. (bvsalud.org)
Microparticles Induce Cell Cycle Arrest Through Redox-Sensitive Processes in Endothelial Cells: Implications in Vascular Senescence. (ohri.ca)

Methods: We performed cell viability and cell-cycle analyses to evaluate the effect of ASC-derived exosomes on the regeneration capacity of cultured hCECs. (bvsalud.org)
Multiple research groups have developed artificial mitochondrial transfer/transplantation (AMT/T) methods that transfer healthy mitochondria into damaged cells and recover cellular function. (biomedcentral.com)

In general, arteries and veins consist of 3 layers, the tunica adventitia, largely constituted by connective tissue and fibroblasts, the tunica media primarily containing vascular smooth muscle cells and also the tunica intima. (adenosine-kinase.com)

It further inhibited cell-cycle progression in the G1 phase by four different mechanisms: rapid downregulation of cyclin D1, induction of Chk2 with simultaneous downregulation of Cdc25A, induction of the Cdk-inhibitor p21 Cip/Waf and inhibition of ribonucleotide reductase activity resulting in reduced dCTP and dTTP levels. (nature.com)
Overall, PERK and IRE1 arms of the UPR are involved in the inhibition of autophagy induced by Simva-TMZ- in GBM cells. (mdpi.com)
Knockdown of Annexin A2 significantly suppressed cell proliferation and invasion of NSCLCs cells, and the biological effect of Annexin A2 on proliferation and invasion was mediated by the inhibition of p53, which in turn, promoted cell cycle progression and EMT. (jcancer.org)

These results reveal that the induction of tip cells and the integration of endothelial guidance cues do not require filopodia. (biologists.com)
MMC also induced phosphorylation of the nuclear histone marker of DNA damage, γH2AX (a member of the H2A histone family), without induction of cell cycle entry or immediate DNA repair measured by Comet assay. (molvis.org)

ASC-derived exosomes protect hCECs against TGF-ß- or H2O2-induced endothelial-mesenchymal transition and mitophagy. (bvsalud.org)
The origin of new endothelial cells (i.e. resident or infiltrating) as well as the underlying mechanism leading to their proliferation (partial endothelial-to-mesenchymal transition [EndMT] or not) have long been debated. (elifesciences.org)
Lung scaffolds were recellularized with mesenchymal stromal cells to assess their biocompatibility. (ibecbarcelona.eu)
Moreover, endothelial cells could undergo a reprogramming procedure toward a mesenchymal phenotype, designated as endothelial-mesenchymal transition, that is characterized by the expression of smooth muscle actin, several fibroblast markers and collagen (328). (adenosine-kinase.com)
Adult mesenchymal stem cells (MSCs) represent a subject of intense experimental and biomedical interest. (biomedcentral.com)
From a research, medical, and business standpoint, mesenchymal stem cell (MSC)-based therapies are fascinating. (biomedcentral.com)
To improve the quality of liver grafts from extended-criteria donors donated after circulatory death (DCD), this study explored whether bone marrow mesenchymal stem cells (BMMSCs) combined with normothermic machine perfusion (NMP) have protective effects on DCD donor livers and the effects of ferroptosis in this procedure. (medscimonit.com)
hC-MSCs were elongated and homogeneously spindle-shaped in morphology with thin cytoplasmic projections (Figure 1H).Human cadaver mesenchymal stromal/stem cell phenotypic and molecular PRMT8 Formulation characterizationAt the third replaying, flow cytometry analysis showed that hC-MSCs expressed recognized markers of hMSCs (CD44, CD73, CD90 and CD105), pericyte antigens (CD146, PDGF-r and NG2) and stemness markers (Stro-1, Oct-4 and Notch-1). (amparinhibitor.com)
The isolated cells also constituting expressed of HLA-G antigen, a well-known tolerogenic molecule involved within the immuomodulatory activity of mesenchymal stromal/stem cells [17] (Figure 2A). (amparinhibitor.com)
Stem Cell Analysis Therapy 2014, 5:eight stemcellres.com/content/5/1/Page 7 ofFigure two Human cadaver mesenchymal stromal/stem cell phenotypic and molecular characterization. (amparinhibitor.com)
A) Representative flow cytometry evaluation of mesenchymal, pericytic, stem cell, hematopoietic and vascular markers. (amparinhibitor.com)
Mechanistically, our studies showed that knockdown of Annexin A2 increased the expression of p53, which in turn, induced cell cycle G2 arrest and inhibited epithelial-to-mesenchymal transition (EMT). (jcancer.org)

The expression of caspase‑3, Bax, Bcl‑2, phosphorylated (p)‑p38MAPK/total (t‑)p38MAPK and p‑endothelial e nitric oxide synthase (eNOS)/t‑eNOS proteins were determined by western blotting. (spandidos-publications.com)

The intermediate filaments Vimentin and Nestin have been strongly expressed virtually in all cells (Figure 2C, D), whereas Neurofilament was optimistic in rare cells. (amparinhibitor.com)
Fluorescent staining of actin filaments in fixed tissue sections and tissue culture cells preparations.Note: Unlike many actin antibodies, Acti-stain™ 555 phalloidin binds only to F-actin resulting in low background fluorescence. (cytoskeleton.com)
Phalloidin has been labeled with a proprietary red fluorescent dye which allows it to be used to stain actin filaments in tissue cultured cells and tissue sections (1, see Figure 1) and cell-free preparations. (cytoskeleton.com)
Acti-stain™ 555 phalloidin-labeled actin filaments retain many functional characteristics of unlabeled actin including their ability to interact with myosin. (cytoskeleton.com)

Around the contrary, no cellsexpressed markers of hematopoietic lineage (CD14 and CD45), hematopoietic progenitor (CD34) or endothelial cells (CD31, vWF). (amparinhibitor.com)
Immunofluorescence: hCMEC/D3 cells stained with ARG52756 anti-CD34 antibody [QBEND/10]. (arigobio.cn)
Immunohistochemical analysis that demonstrates the endothelial nature of the neoplasm is frequently positive for specific vascular markers (CD31, CD34, factor VIII), and keratin 1,2,5,6 . (bvsalud.org)

Studies have shown that periodontitis is associated with altered expression of inflammatory mediators in gingival fibroblasts and periodontal membrane cells ( 3 , 4 ). (spandidos-publications.com)
These growth factors act on surrounding cells and stimulate chemotaxis of neutrophils, monocytes, and fibroblasts to the area of injury. (medscape.com)
bFGF is a chemotactic and mitogenic factor for fibroblasts and endothelial cells. (medscape.com)

Stable F98 glioma cell lines with MCT4 overexpression, normal expression, and knockdown were generated. (hindawi.com)

This process enables tumor cells, including those within the core of the heterogeneous tumor mass to survive, despite being at a distance from blood vessels that supply their metabolic needs [ 16 , 17 ]. (hindawi.com)
Recently, we found that decorin evokes a protracted autophagic program in endothelial cells mitochondrial autophagy in tumor cells. (jefferson.edu)

Along with flow cytometry evaluation, a single immunofluorescence staining was performed to investigate the smooth muscle (-smooth muscle actin, calponin, hcaldesmon, Vimentin and Desmin) and neural (NSE, Nestin, Neurofilament and S100) phenotypes. (amparinhibitor.com)

In contrast, Akt-phosphorylation, β-catenin, and c-Myc are repressed in the cells, presumably inhibiting tumor growth. (amegroups.org)
The tumor-promoting signals such as Akt-phosphorylation, β-catenin, and c-Myc were upregulated in the cells compared to the controls. (amegroups.org)
2004) Akt phosphorylation and gefitinib efficacy in patients with advanced non-small-cell lung cancer. (scielo.br)

We found that most of the previously described PAR 1 -targeted agents can also activate/desensitize PAR 2 , and most of these peptides can also activate a calcium signaling pathway in a target cell that possesses PAR 2 along with PAR 1 . (aspetjournals.org)

This study describes the first comparative phosphoproteome analysis of growing erythroid cells (gECs), derived from human haematopoietic stem cells, exposed to lysates of infected erythrocytes (IE)/uninfected erythrocytes (UE) for 24, 48 and 72 h. (biomedcentral.com)
In the field of stem cells, cumulative evidence has shown that mitochondrial transfer contributes to stem cell-triggered repair of damaged cells [ 9 ]. (biomedcentral.com)
Although hematopoietic stem and progenitor cells (HSPCs) enable autologous treatment of blood disorders, de novo generation of hematopoietic cells from pluripotent stem cells still compromises the unlimited high yield and rich hematopoiesis engraftment potential [ 2 ]. (biomedcentral.com)
Cancer stem cells (CSCs) and poorly differentiated progenitor cells in pancreatic ductal adenocarcinoma (PDAC) exacerbate hierarchical tissue organization contributing to cell hyperproliferation and therapy resistance. (amegroups.org)

These MSC influences can range from relatively rare activities that require cell contact, such as mitochondrial transfer and cell fusion, to relatively common paracrine MSC actions through extracellular microvesicles or secreted factors. (biomedcentral.com)
Time-lapse videography of living cells captures spectacular intracellular mitochondrial movement, and this movement promotes mitochondrial connections to form a dynamic mitochondrial network [ 3 ]. (biomedcentral.com)
In addition to intracellular mitochondrial movement, intercellular mitochondrial transfer between mammalian cells has recently been discovered. (biomedcentral.com)
Intercellular mitochondrial transfer can be regarded as an extension of intracellular mitochondrial movement or intercellular communication, which undoubtedly increases the mtDNA content of the recipient cells and restores the respiration and survival of the recipient cells. (biomedcentral.com)

Finally, we correlated the NP functionality, cell receptor density, and NP uptake to identify the highest cell uptake selectivity regimes. (ibecbarcelona.eu)
However, this paradigm changed upon the discovery that soluble decorin directly binds, with high affinity, to a plethora of cell surface receptor tyrosine kinases that are richly expressed by the tumor microenvironment and parenchyma. (jefferson.edu)
We sought to extend the receptor-desensitization paradigm for use with a cultured cell system, in which an intracellular calcium signal rather than a contractile response might be used as an index of receptor activation. (aspetjournals.org)
2004) Activating mutations in the epidermal growth factor receptor underlying responsiveness of non-small-cell lung cancer to gefitinib. (scielo.br)
Metz H, Busch SE, Hanke ML, Kargl J, Kim KH and Houghton M (2014) Insulin receptor substrate-1 regulates immune cell content in lung adenocarcinoma. (scielo.br)

We developed a calcium signaling-based assay, using cultured human embryonic kidney cells (HEK), that evaluates simultaneously, the activation/desensitization or blockade of the proteinase-activated receptors, PAR 1 and PAR 2 . (aspetjournals.org)
To this end, we developed a calcium-signaling assay, employing cultured human embryonic kidney cells (HEK293) in which the action of PAR 1 and PAR 2 agonists and antagonists could be evaluated simultaneously. (aspetjournals.org)

Mouse ovarian ECs were isolated using magnetic cell sorting or by FACS and cultured in serum free media. (biomedcentral.com)
After a constitutive activation of the Akt pathway was initiated, early follicles (50-150 um) were mechanically isolated from 8-day-old mice and co-cultured with these activated ovarian endothelial cells (AOEC) ( n = 32), gel ( n = 24) or within matrigel ( n = 27) in serum free media for 14 days. (biomedcentral.com)
The potential clinical applications of research directed on corpus luteum endothelial cells are intriguing considering reproductive processes in which vascular dysfunctions may play a role such as ovarian failure, polycystic ovary syndrome (PCOS), and ovarian hyperstimulation syndrome (OHSS). (biomedcentral.com)

The ability of two cytokines, tumor necrosis factor alpha and interferon gamma, are evaluated as paracrine mediators of endothelial cell function during angioregression. (biomedcentral.com)

At a molecular level, the number of functional antibodies on the NP surface and the density of receptors on the target cell determine the targeting interaction. (ibecbarcelona.eu)
Here, we developed a labeling approach to quantify the number of functional cetuximabs conjugated to NPs and the expression of epidermal growth factor receptors (EGFRs) in breast cancer cells using direct stochastic optical reconstruction microscopy (dSTORM). (ibecbarcelona.eu)
The single-molecule resolution of dSTORM allows quantifying molecules at the nanoscale, giving a detailed insight into the distributions of individual NP ligands and cell receptors. (ibecbarcelona.eu)
With both receptors present in the same cell, the selectivity or nonselectivity of a variety of compounds that would affect PAR 1 and/or PAR 2 could be efficiently evaluated in a single experiment. (aspetjournals.org)
Introduction For some retroviruses, the viral envelope binds to receptors within a pH-independent way, recommending which the virions may fuse towards the cell membrane [1] straight. (angiogenesis-blog.com)
As a result, viral receptors over the cell membrane offer binding sites for the trojan and so are also mixed up in structural modulation of viral envelopes, resulting in the fusion Pseudouridimycin from the viral and mobile membranes and virion entrance, the first step in viral an infection of focus on cells [2]. (angiogenesis-blog.com)
As opposed to almost every other lentiviruses, such as for example human immunodeficiency trojan (HIV)-1, simian immunodeficiency trojan (SIV) and feline immunodeficiency trojan (FIV), which need co-receptors for effective an infection, EIAV seems to depend just on an operating ELR1 for the invasion of focus on cells. (angiogenesis-blog.com)

NSCLCs including adenocarcinoma, squamous cell carcinoma and large cell carcinoma, constitute 80% of all lung cancers [ 2 ]. (jcancer.org)
Cancer Lett, 300:215-224.doi:10.1016/j. squamous cell carcinoma in north-eastern Iran. (who.int)

In summary, our data highlight MCT4 /SLC16A3 as a key gene for distinct hallmarks of tumor malignancy in glioma cells. (hindawi.com)

Investigating the downstream transduction pathways revealed key molecular targets underpinning decorin-mediated cell cycle arrest and tumorigenic and angiogenic suppression. (jefferson.edu)
Although studies have identified unique transcription factors in the selection of normal pancreatic lineages, the cells of origin and molecular mechanisms arbitrating PDAC growth are poorly defined. (amegroups.org)

En face co-immunostaining of the mouse aortic arch revealed a low level of PDCD4 in endothelial cells undergoing pulsatile shear stress. (plos.org)

The resulting acellular lung scaffolds were shown to be biocompatible (98% cell survival after 72 h of culture). (ibecbarcelona.eu)

Evidence of proliferating endothelial cells was determined by the presence of Ki-67 antigen-positive cells, bromodeoxyuridine-positive cells, or [ 3 H] thymidine-positive cells. (biomedcentral.com)

Live imaging identifies dynamic and transient F-actin-based structures, such as filopodia, contractile ring and cell cortex, and more persistent F-actin-based structures, such as cell junctions. (biologists.com)

Annexin A2 has been involved in cancer cell adhesion, invasion and metastasis. (jcancer.org)
and platelet/endothelial cell adhesion molecule-1, PECAM-1). (biomedcentral.com)

For functional analysis, we used low concentrations of Latrunculin B that preferentially inhibited F-actin polymerisation in filopodia. (biologists.com)

Chronic metabolic disorders, such as hyperglycemia and dyslipidemia, promote endothelial dysfunction and eventually leakage, resulting in increased monocyte diapedesis ( 5 - 7 ). (spandidos-publications.com)

Addition of 237.9 µmol/L UA improved cellular damage and oxidative stress in H2O2-treated H9C2 cells, as determined by cell viability, LDH release, ROS levels, and total antioxidant capacity assays. (bvsalud.org)
Cell viability was assessed using the Cell Counting Kit‑8 (CCK‑8) assay. (spandidos-publications.com)

Programmed cell death 4 (PDCD4) is an important tumor suppressor in the development of various human cancers [1] and inhibits translation rather than transcription. (plos.org)
Using human retinal endothelial cells in 20 mM D-glucose, H3K9me3 at Rac1 promoter was quantified by chromatin-Immunoprecipitation technique. (nature.com)
These human RNAs Do a removing download Ethos and see cities, overhangs, cells, ATPases operators, 7SL RNA, 7SK RNA, the RNA cell of RNase transfer RNA, the RNA number of RNase MRP, and the RNA molecule of conservation. (evakoch.com)
We isolated CSCs from low passage PDAC cell lines derived from human tumors and commercially available cell lines. (amegroups.org)
The abilities of the PAR 1 -targeted agents to desensitize or block PAR 1 in the HEK cell assay were compared with their activities in a human platelet aggregation assay. (aspetjournals.org)
Results showed that in cultured human-derived retinal pigment epithelial ARPE-19 cells , the extract of Chlorella zofingiensis and its nutritional ingredient astaxanthin exhibited significant inhibitory effects on the formation of endogenous N ε -carboxymethyllysine (CML), a key AGE representative, through the suppression of intracellular oxidative stress. (rsc.org)

Endothelial dysfunction during diabetes has been previously reported to be at least in part attributed to increased oxidized low‑density lipoprotein (oxLDL) levels mediated by high glucose (HG) levels. (spandidos-publications.com)
These results suggest that rosmarinic acid can exert a protective effect against oxLDL‑mediated endothelial dysfunction under HG conditions by reducing the interaction between monocytes and ECs in addition to preventing monocyte diapedesis. (spandidos-publications.com)
Endothelial dysfunction during diabetes is closely associated with increased oxidized low-density lipoprotein (oxLDL) levels mediated by high glucose (HG) conditions ( 8 ). (spandidos-publications.com)

Isotype controls are presented as filled black histograms, the precise cell markers as white histograms. (amparinhibitor.com)

IL-2 promotes further T cell proliferation to augment the immunogenic response to injury. (medscape.com)

Crosstalk between H3K9me3 and DNA methylation was examined in cells transfected with siRNA of histone trimethyl-transferase, Suv39H1, or Dnmt1 , exposed to high glucose. (nature.com)

Thus, the secretion of gall wasps stimulates plant cell growth and overrules homeostasis of the affected leaf area - this is similar to tumour outgrowth. (nature.com)

Endothelial cells communicate with cells comprising the functional unit of the corpus luteum, i.e ., other vascular cells, steroidogenic cells, and immune cells. (biomedcentral.com)

Phalloidin is a seven amino acid peptide toxin from the mushroom Amanita phalloides, which binds specifically and with high affinity (Kd 20 nM) to the polymerized form of actin (F-actin). (cytoskeleton.com)

EPCs from CB expressed abundant genes involved in cell cycle, hypoxia signalling and blood vessel development, correlating with the phenotypes that CB-EPCs proliferated more rapidly, migrated faster, and formed tubule structure more efficiently. (biomedcentral.com)
These cells may be activated by a wide range of stimuli, such as immunological, inflammatory or bacterial components, to express a diversity of "early immediate genes", including Tissue Factor (TF) and Tumour Necrosis Factor a (TNFa) (1-3). (thermofisher.com)

The cells are usually large and polygonal, containing abundant eosinophilic cytoplasm, a large nucleus with open chromatin, a prominent eosinophilic nucleolus, and an occasional intracellular lumina containing red blood cells 1,4-7 . (bvsalud.org)

The complex PDAC pathobiology is established predominantly through KRAS mutations and the associated cellular signaling that contributes to cell proliferation and dedifferentiation ( 5 , 6 ). (amegroups.org)

Anatomy39

Organisms11

Diseases4

Chemicals and Drugs116

Analytical, Diagnostic and Therapeutic Techniques and Equipment25

Phenomena and Processes59

Disciplines and Occupations1

Technology, Industry, Agriculture1

Information Science3

Cytoskeleton7

Angiogenesis14

Vitro14

Microvascular endothelial cells2

Migration8

Monolayer6

Umbilical5

MRNA7

VEGF6

Progenitor cell3

Apoptosis11

Vivo6

Stress fibers2

Kinase5

Corneal3

Inflammation3

Regulation8

Tissues4

Differentiation6

Protein6

Atherosclerosis7

Senescence2

Methods2

Vascular smooth muscl1

Inhibition3

Induction2

Mesenchymal12

Nitric1

Filaments4

CD343

Fibroblasts3

Overexpression1

Tumor cells2

Calponin1

Phosphorylation3

Signaling pathway1

Stem Cells4

Mitochondrial4

Receptor5

Embryonic2

Ovarian3

Mediators1

Receptors7

Squamous cell carc2

Gene1

Molecular2

Aortic1

Survival1

Evidence1

Transient1

Adhesion2

Preferentially1

Hyperglycemia1

Viability2

Human6

Dysfunction3

Markers1

Promotes1

SiRNA1

Homeostasis1

Functional1

Binds1

Genes2

Nucleolus1

Predominantly1