Endothelial cell hypoxia. Medical search. Frequent questions

However, its role has not been evaluated in hypoxic choroidal endothelial cells (CECs), the precursor of VEGF-mediated choroidal angiogenesis. (molvis.org)
Mammalian cells respond to hypoxia by modulating oxygen-sensing transducers that stabilize the transcription factor hypoxia-inducible factor 1α (HIF-1α), which transactivates genes governing angiogenesis and metabolic pathways. (umn.edu)
van Nieuw Amerongen, G. P., Koolwijk, P., Versteilen, A. & van Hinsbergh, V. W. Involvement of RhoA/Rho kinase signaling in VEGF-induced endothelial cell migration and angiogenesis in vitro. (nature.com)
These cells can be differentiated into mature endothelial cells, which are involved in processes of angiogenesis and vessel regeneration. (intechopen.com)
This chapter provides an overview of the key role of hEPC in promoting angiogenesis and their potential use for cell therapy. (intechopen.com)
In addition, cell proliferation and angiogenesis will be stimulated, enabling better oxygenation of the tissue. (bmj.com)
Although vascular endothelial growth factor (VEGFA-165) is primarily known for its role in angiogenesis, it also plays important neurotrophic and neuroprotective roles for spinal motor neurons. (jneurosci.org)
In vitro and in vivo studies have shown that VEGF is responsible for stimulating the proliferation of microvascular endothelial cells, inducing angiogenesis and rendering the microvasculature hyperpermeable. (cdc.gov)
Hypertension impairs hypoxia-induced angiogenesis in men. (bvsalud.org)
These results suggest that hypertension -induced impairment in angiogenesis in response to isocapnic hypoxia is related to disrupted NO bioavailability , VEGF chemotactic signaling, and EPC mobilization. (bvsalud.org)
It acts by inducing angiogenesis and endothelial cell migration. (bvsalud.org)
Although tumor-associated angiogenesis has traditionally been defined as the sprouting of new vessels from preexisting vessels, it is becoming clear that the blood vessels that support tumor growth can also originate from cells recruited from the bone marrow or can even differentiate from tumor stem cells (vascular mimicry). (medscape.com)
The critical initial stimulus for angiogenesis is hypoxia in the growing tumor. (medscape.com)

Induction of hypoxia was confirmed by flow cytometric analysis and the levels of SIRT1 were noted in a hypoxic condition as well in the cells after blocking SIRT1 activity using sirtinol. (molvis.org)
Hypoxic induction was confirmed using flow cytometric analysis, which showed cell cycle arrest starting at a 200 µM concentration of cobalt chloride. (molvis.org)
In this study, we aimed to examine the effects of RCE in endothelial cells challenged with hypoxic exposure and to elucidate the underlying mechanisms. (mdpi.com)
Pyroptosis is a form of programmed cell death involved in the pathophysiological progression of hypoxic pulmonary hypertension (HPH). (biomedcentral.com)
The expression levels of FENDRR in hypoxic pulmonary artery endothelial cells (HPAECs) were detected by using quantitative real-time polymerase chain reaction (qRT-PCR) and fluorescence in situ hybridization (FISH). (biomedcentral.com)
Estrogen treated TG1-1 cells secreted higher levels of VEGF which were comparable to secreted levels from cells grown under hypoxic conditions. (biomedcentral.com)
To determine the cause of decreased arachidonic acid release, we pulse-labeled BAEC with [ 14 C]arachidonic acid for 5 min under normoxic conditions and chased cells for 1 h under normoxic or hypoxic conditions. (elsevierpure.com)
There was no relevant difference between normoxic and hypoxic cells in the metabolism of any of the arachidonyl molecular species of diacyl lipids. (elsevierpure.com)
However, there was a marked decrease (~40%) in the turnover of arachidonyl alkenylacyl phosphatidylethanolamine in the hypoxic cells. (elsevierpure.com)
Neutrophil adhesion to ECV304 cells, which had been hypoxic for 4 hours and then reoxygenated for 30 minutes, was significantly increased. (bl.uk)
When neutrophils were exposed to both hypoxic/reoxygenated endothelial cells and the soluble factor (s) released by them a large oxidative burst was elicited. (bl.uk)
Analysis of the supernatant from hypoxic/reoxygenated endothelial cell cultures and studies using inhibitors of secretion suggested platelet activating factor (PAF) may be a major component in this overall sequence of events. (bl.uk)
Here we investigate the role of p22phox, a regulatory subunit of Nox, in COPD lungs, hypoxic pulmonary vasoconstriction (HPV), hypoxia-induced pulmonary vascular remodelling and pulmonary hypertension. (ersjournals.com)
BNP also stimulated the proliferation of WT1 + epicardium-derived cells but only in the hypoxic area of infarcted hearts. (elifesciences.org)
The formation of new vessels in the hypoxic area restores blood flow, provides oxygen and nutriments to the surviving cells, and promotes the migration and engraftment of new cells. (elifesciences.org)
A hypoxic environment devoid of nutrients prevents the cell undergoing energy dependent apoptosis and cells become necrotic. (bmj.com)
Low ATP concentrations in hypoxic tumour cells disable the apoptotic cascade and induce cell death by necrosis. (bmj.com)
Exosomes reflect the hypoxic status of glioma cells and mediate hypoxia-dependent activation of vascular cells during tumor development. (lu.se)

However, the effects of RCE on response to hypoxia in the endothelium remain unclear. (mdpi.com)
The expression of VEGF is potentiated and is secreted by tumor cells in response to hypoxia, by activated oncogenes, and by a variety of cytokines. (sigmaaldrich.com)
Apoptosis can be induced in response to hypoxia. (bmj.com)
A circulating angiopoietin-like protein that is expressed in a variety of tissues in response to HYPOXIA. (bvsalud.org)
However, changes in cell signalling in response to hypoxia that are mediated by RGS5 stroke. (lu.se)

Activated SIRT1 signals hypoxia inducible factor (HIF)-2α, which transactivates vascular endothelial growth factor (VEGF) and erythropoietin. (molvis.org)
Our study results demonstrate that hypoxia mimetic cobalt chloride induces SIRT1 and augments HIF-2α, which activates and releases VEGF. (molvis.org)
Previously we found that estrogen aided in this neovasculogenesis via recruitment of bone marrow derived endothelial progenitor cells (BM-EPCs), leading to increased vessel formation and vascular endothelial growth factor (VEGF) production in vivo . (biomedcentral.com)
We investigated the influence of alpha6beta1 on vascular endothelial growth factor (VEGF) expression because autocrine VEGF is necessary for the survival of serum-deprived cells in hypoxia. (umassmed.edu)
The results obtained indicate that alpha6beta1 is necessary for VEGF expression because the ability of hypoxia to activate HIF-1 and to stimulate VEGF transcription in MDA-MB-435 cells is dependent on alpha6beta1 expression by a mechanism that involves protein kinase C-alpha. (umassmed.edu)
The most widely studied serum markers for PE, to date, are vascular endothelial growth factor (VEGF) and placental growth factor (PlGF). (hindawi.com)
VEGF (Vascular endothelial growth factor, VEGFA, VEGF-A), a dimeric ligand, is among the most potent angiogenic mitogens. (sigmaaldrich.com)
VEGF is secreted by tumor cells and other cells exposed to hypoxia. (sigmaaldrich.com)
VEGF is a highly specific mitogen for vascular endothelial cells. (sigmaaldrich.com)
Western Blot Analysis: A 1:500 dilution of this antibody detected VEGF on 10 µg of NIH/3T3 cell lysate. (sigmaaldrich.com)
One may speculate that, early after the development of RVO, systemic ET-1 level may increase, then decrease later till the onset of retinal neovascularisation, which might induce an increase again possibly via an increased expression of vascular endothelial growth factor (VEGF), since VEGF can cause an increased expression of endothelin converting enzyme. (bmj.com)
Vascular Endothelial Growth Factor (VEGF) is one of the most important angiogenic components of tissue healing. (cdc.gov)
In the tendon, expression of VEGF can be up-regulated by both mechanical and biochemical stimuli that includes hypoxia and the presence of other growth factors. (cdc.gov)
The purpose of this study was to evaluate the regional variation of VEGF staining cells in the FDP tendon at the epicondyle in response to repetitive loading. (cdc.gov)
endothelial activation, CD62E/CD144), nitrite , vascular endothelial growth factor ( VEGF ), and stromal cell-derived factor 1 (SDF-1) were measured. (bvsalud.org)
During normoxia, EPCs, nitrite , endothelial activation, and SDF-1 were similar between groups, whereas VEGF was lower (Pâ =â 0.02) and apoptotic EMVs tended to increase (Pâ =â 0.07) in hypertensive men . (bvsalud.org)
Results: In BEAS-2B and hAELVi, hypoxia significantly dowregulated genes related to fibrosis, mitochondrial stress, oxidative stress, apoptosis and inflammation whereas VEGF receptor 2 increased. (lu.se)
Hypoxia increased the expression of Tenascin-C, whereas both hypoxia and TGF-β1 stimuli increased the release of VEGF, IL-6, IL-8 and MCP-1 in BEAS-2B. (lu.se)

The aim of this study was to assess whether activation of nrf2 by sulforaphane in human microvascular endothelial cells prevents metabolic dysfunction in hyperglycemia. (diabetesjournals.org)

Fig. 5: RhoA induces glucose transporter SLC2A3 translocation which drives subcellular glycolysis in thrombin-stimulated contractile endothelial cells. (nature.com)
In addition, hypoxia induces the endothelial expression of adhesion molecules, including von Willebrand factor (VWF). (usp.br)
Here, we find that chemotherapy induces the expression of glutathione S-transferase omega 1 (GSTO1), which is dependent on hypoxia-inducible factor 1 (HIF-1) and HIF-2. (nih.gov)
2 In addition, hypoxia induces genetic instability by the induction of fragile sites causing gene amplification. (bmj.com)

Human endothelial progenitor cells (hEPCs) are adult stem cells, located in the bone marrow and peripheral blood. (intechopen.com)
Different phenotypes and subtypes of endothelial progenitor cells (EPCs), such as early and late EPCs, have been described according to their functionality. (intechopen.com)

Using the murine mammary cell line TG1-1 we observed estrogen (E 2 ) lead to an up regulation of hypoxia inducible factor-1 (HIF-1), an effect abrogated by the anti-estrogen Fulvestrant and the HIF-1 inhibitor YC-1 (3-(5'-hydroxymethyl-2'-furyl)-1-benzylindazole) suggesting the interchangeability of hypoxia and estrogen mediated effects. (biomedcentral.com)
Interestingly, estrogen is also capable of contributing to breast cancer progression by a novel role, via modulation of proteins involved in hypoxia signaling, namely hypoxia inducible factor 1 (HIF-1). (biomedcentral.com)
Mechanical activation of hypoxia-inducible factor 1α drives endothelial dysfunction at atheroprone sites. (nature.com)
The key regulator of this process, hypoxia inducible factor 1 (HIF-1), can initiate apoptosis by inducing high concentrations of proapoptotic proteins, such as BNIP3, and can cause stabilisation of p53. (bmj.com)
Polymorphisms of the hypoxia-inducible factor 1 gene and peripheral artery disease. (cdc.gov)

These data demonstrate that pulmonary vascular endothelial cells respond to hypoxia by reversibly decreasing ET-1 production, and this attenuation is likely regulated at the level of transcription. (johnshopkins.edu)
The inability of the organism to appropriately respond to hypoxia results in abnormal cell metabolism and function. (bvsalud.org)

abstract = "Adaptive changes to oxygen availability are critical for cell survival and tissue homeostasis. (umn.edu)

One complementary therapy could be 're-activating' vasculogenesis (i.e. the differentiation of precursor cells into mature endothelial cells), a mechanism that occurs in the heart during development but is quiescent in adult hearts. (elifesciences.org)

Methods were initially developed to examine the adhesion of neutrophils to monolayers of a cultured endothelial cell line (ECV304) after periods of hypoxia and reoxygenation. (bl.uk)
Cells adapt to this environmental stress, so that after repeated periods of hypoxia, selection for resistance to hypoxia induced apoptosis occurs. (bmj.com)

A current hypothesis unifies these concepts where an altered immune response leads to disturbed placental function early in pregnancy with consequent syncytiotrophoblast ischemia and shedding of products that extensively damage endothelial integrity. (hindawi.com)
Burns, hypo- and hyperthermia, rhabdomyolysis, and hypoxia damage endothelial cells and allow for the exposure of tissue factor. (vin.com)

Reductions in uteroplacental flow initiate a cascade of molecular effects leading to hypoxia, thrombosis, inflammation, and endothelial cell dysfunction resulting in untoward pregnancy outcomes. (hindawi.com)
LINC00926 is involved in hypoxia-induced vascular endothelial cell dysfunction via miR-3194-5p regulating JAK1/STAT3 signaling pathway. (nih.gov)
CONCLUSIONS- We conclude that activation of nrf2 may prevent biochemical dysfunction and related functional responses of endothelial cells induced by hyperglycemia in which increased expression of transketolase has a pivotal role. (diabetesjournals.org)

Sleep apnea is a risk factor for cardiovascular disease, and intermittent hypoxia (IH, 20 episodes/h of 5% O2-5% CO2 for 7 h/day) to mimic sleep apnea increases blood pressure and impairs hydrogen sulfide (H2S)-induced vasodilation in rats. (unboundmedicine.com)
In silico analysis identified putative nuclear factor of activated T cell (NFAT) binding sites in the CSE promoter. (unboundmedicine.com)
The observations that IH exposure decreases NFATc3 activation and CSE-dependent vasodilation support a role for NFAT in regulating endothelial H2S production.NEW & NOTEWORTHY This study identifies the calcium-regulated transcription factor nuclear factor of activated T cells as a novel regulator of cystathionine γ-lyase (CSE). (unboundmedicine.com)
This pathway is basally active in mesenteric artery endothelial cells, but, after exposure to intermittent hypoxia to mimic sleep apnea, nuclear factor of activated T cells c3 nuclear translocation and CSE expression are decreased, concomitant with decreased CSE-dependent vasodilation. (unboundmedicine.com)
Hypoxia is a critical pathological factor in a variety of retinal diseases, including age-related macular degeneration. (molvis.org)
The role of SIRT1 in the activation of HIF-2α and nuclear factor-κB (RelA/p65) during hypoxia in the presence or absence of SIRT1 was assessed using immunoblot analysis. (molvis.org)
Bovine pulmonary artery (BPAE) and microvascular endothelial (BMVE) cells were isolated, grown in tissue culture, and characterized by the presence of Factor VIII related antigen and LDL uptake. (johnshopkins.edu)
To understand the functional interactions between the TrkA and p75 nerve growth factor (NGF) receptors, we stably transfected LAN5 neuroblastoma cells with an expression vector for ET-R, a chimeric receptor with the extracellular domain of the epidermal growth factor receptor (EGFR), and the TrkA transmembrane and intracellular domains. (umassmed.edu)
Notably, RhoB was specifically required for hypoxia-induced factor-1α stabilization and for hypoxia- and platelet-derived growth factor-induced cell proliferation and migration. (brighton.ac.uk)
Similarly, sEng is a truncated form of receptor for two subtypes of transforming growth factor beta (TGF β ) specifically, TGF β 1 and TGF β 2 which are highly expressed by vascular endothelial cells and syncytiotrophoblasts. (hindawi.com)
ROS generation in turn caused the release of a soluble factor (s) which induced a conformational change on the neutrophil surface allowing it to bind to the intercellular adhesion molecule 1 (ICAM-1) on the endothelial cell. (bl.uk)
Soluble factor (s) from hypoxia/reoxygenated endothelial cells also had a powerful neutrophil chemoattractant ability. (bl.uk)
The NAPDH oxidase (Nox) family is emerging as a key disease-related factor in vascular diseases, but currently its role in hypoxia-induced pulmonary remodelling in COPD remains unclear. (ersjournals.com)
Emphysema formation has been linked to the involvement of the small pulmonary arteries and the endothelium and could be achieved experimentally using vascular endothelial growth factor antagonists [ 3 ] or by chronic smoke exposure [ 4 ]. (ersjournals.com)
De- cidual cells promote hemostasis through enhanced expression of tissue factor (TF), the primary initiator of hemostasis via thrombin generation, and plasminogen activator inhibitor-1, which inactivates tissue type plasminogen activator, the primary fibrinolytic agent. (scirp.org)
Levy, A.P., Levy, N.S. and Goldberg, M.A. (1996) Post-transcriptional regulation of vascular endothelial growth factor by hypoxia. (scirp.org)
2009) Progestin and thrombin regulate tissue factor expression in human term decidual cells. (scirp.org)
Here, we report that in AD patients and two mouse models of AD, overexpression of serum response factor (SRF) and myocardin (MYOCD) in cerebral vascular smooth muscle cells (VSMCs) generates an Aβ non-clearing VSMC phenotype through transactivation of sterol regulatory element binding protein-2, which downregulates low density lipoprotein receptor-related protein-1, a key Aβ clearance receptor. (nature.com)
Especially common in gram-negative sepsis where endotoxin stimulates monocytes and endothelial cells to express tissue factor. (vin.com)
Malignant cells cause endothelial damage and allow the expression of tissue factor as well as other procoagulant materials. (vin.com)
Commonly seen in hemangiosarcomas because of damaged endothelial cells, release of tissue factor. (vin.com)
Endothelial cells can be damaged and tissue factor released. (vin.com)
Other findings include the presence of fragmented red cells and low specific clotting factor assays for Factors II, V, VII, VIII, and IX. (vin.com)
The localization of vascular endothelial growth factor in a repetitively loaded tendon in vivo: an immunohistological study. (cdc.gov)
The development of the tumor vasculature is dependent on the homeostatic balance between a variety of proangiogenic and antiangiogenic (vascular endothelial growth factor and thrombospondin, respectively), inflammatory, and coagulation factors. (medscape.com)
LRG1 promotes endothelial cell proliferation and migration by binding directly to transforming growth factor- β (TGF- β ) accessory receptor endoglin, which, in the presence of TGF- β , enhances the activation of the proangiogenic Smad1/5/8 signaling pathway. (medscape.com)

9. Chan CK, Vanhoutte PM. Hypoxia, vascular smooth muscles and endothelium Acta Pharmaceutica Sinica B. 2013;3(1):1-7. (scielo.br)
This injury appears to be a consequence of the host's immunological response to viral antigens that have penetrated the endothelium by means of the cells' own integrins. (medscape.com)

Soluble factors in conditioned media from E 2 treated breast cancer cells also lead to migration and tube formation of human umbilical vein endothelial cells (HUVEC) in vitro . (biomedcentral.com)
We show that cellular glycolysis fuels endothelial activation, migration and contraction and that sites of high lactate production colocalize with active cytoskeletal remodelling within an endothelial cell. (nature.com)
Neutrophil migration in response to factors secreted by the treated endothelial cells was then assessed. (bl.uk)
The timeline for cell migration in a normal wound healing process is predictable. (medscape.com)
Enstrom A, Carlsson R, Ozen I, and Paul G. RGS5: a novel role as a hypoxia-responsive protein that suppresses chemokinetic and chemotactic migration in brain pericytes. (lu.se)
Cancer is a complex disease in which many basic processes, such as cell division, apoptosis, and cell migration are dysregulated. (medscape.com)

At the onset of acute hypoxia, eicosanoid synthesis by bovine aortic endothelial cells (BAEC) markedly decreases, reflecting a decreased release of arachidonic acid from endogenous stores. (elsevierpure.com)
RhoB deficiency in mice markedly attenuated development of chronic hypoxia-induced pulmonary hypertension, despite compensatory expression of RhoA in the lung.CONCLUSIONS: RhoB mediates adaptational changes to acute hypoxia in the vasculature, but its continual activation by chronic hypoxia can accentuate vascular remodeling to promote development of pulmonary hypertension. (brighton.ac.uk)

There could be thickening of the vessels and they're more sensitive to vasospasm, which leads to tissue hypoxia and injury. (hstalks.com)
and 5) absence of severe respiratory illness (to concurrently received positive test results for SARS-CoV-2, exclude patients in which inflammation and organ dysfunc- the virus that causes COVID-19, by polymerase chain reaction tion might be attributable simply to tissue hypoxia). (cdc.gov)

Pericytes are perivascular cells that line the entire microvasculature. (lu.se)
Upon brain hypoxia, pericytes are one of the first responders at the blood/brain interface. (lu.se)
Human brain vascular pericytes treated with either scrambled or RGS5 targeting siRNA, will be co-cultured in a spheroid model of the BBB together with human brain endothelial cells and human astrocytes. (lu.se)
We have established a method allowing to study the cell-specific secretome of pericytes in vitro-using Turbo-ID, a mutant non-toxic biotin ligase (6). (lu.se)

We examined changes in endothelial biomarkers and vascular chemoattraction/angiogenic capacity in response to isocapnic hypoxia in hypertensive men . (bvsalud.org)

7 Severe hypoxia in the presence of energy stimulates cells to undergo apoptosis, whereas oxygen levels above 0.5% prevent cell death. (bmj.com)
There is some sort of etiologic agent, which we do not know what it is, which stimulates or activates the molecular and cellular target cells in a patient, who has the specific genetic background for this disease. (hstalks.com)

Rhodiola crenulata root extract (RCE) has been shown to possess protective activities against hypoxia both in vitro and in vivo. (mdpi.com)
MDA-MB-435 cells engineered to be deficient in alpha6beta1 expression form primary tumors that are highly apoptotic and unable to metastasize, although they exhibit no increased apoptosis in vitro under standard culture conditions. (umassmed.edu)
Although other irritants in the smoke may have contributed to the incident, there is supporting evidence that stable strontium can stimulate the release of histamine from mast cells in vitro (ATSDR 2001e). (cdc.gov)

The enzyme that produces H2S, cystathionine γ-lyase (CSE), is decreased in rat mesenteric artery endothelial cells (EC) following in vivo IH exposure. (unboundmedicine.com)
The genesis of an inappropriate oxidative burst by the neutrophil upon exposure to endothelial chemoattractants and adhesion molecules was also measured. (bl.uk)
Exposure of breast cancer cells to chemotherapy leads to an enrichment of BCSCs. (nih.gov)

2. The aim of this work was to investigate how hypoxia affects human lung epithelial cells in combination with profibrotic stimuli and its correlation to pathogenesis. (lu.se)
Discussion: In conclusion, our data indicate that bronchial and alveolar epithelial cells respond differently to hypoxia and profibrotic stimuli. (lu.se)

Cells were also treated with PKC activator PEP005 or specific siRNA against protein kinase Cδ (PKCδ). (medscimonit.com)
Estrogen modulation of HIF-1 and subsequent effects on endothelial cells is dependent on the Akt/PI3K pathway and protein synthesis as validated by the use of the inhibitors wortmannin and cycloheximide which abrogated estrogen's effects respectively. (biomedcentral.com)

After a short period, capillary vasodilatation occurs secondary to local histamine release, and the cells of inflammation are able to migrate to the wound bed. (medscape.com)

Here, we use phrenic motor facilitation (pMF) as a general term, including pLTF after acute intermittent hypoxia and other forms induced, for example, by spinal administration of receptor agonists. (jneurosci.org)
Intermittent hypoxia-induced pLTF is associated with greater ERK phosphorylation (activation) in the region of the phrenic motor nucleus ( Wilkerson and Mitchell, 2009 ), and pLTF requires ERK (not Akt) activation in its underlying mechanism (M. S. Hoffman and G. S. Mitchell, unpublished observation). (jneurosci.org)

Directly linking cell motility to cell metabolism is technically challenging but biologically important. (nature.com)
Our data indicate that Rho-GTPase signalling coordinates energy metabolism with cytoskeleton remodelling to regulate endothelial cell motility. (nature.com)
Fig. 3: Single-cell metabolism links migratory cell phenotypes to glycolysis states. (nature.com)
Laminar shear stress inhibits endothelial cell metabolism via KLF2-mediated repression of PFKFB3. (nature.com)

LINC00926 promotes pyroptosis of hypoxia-induced human umbilical vein vascular endothelial cells by recruiting ELAVL1]. (nih.gov)

These results demonstrate that TrkA kinase initiates differentiation, and p75 enhances differentiation by rescuing differentiating cells from apoptosis via the PI3K pathway. (umassmed.edu)
3- 5 Therefore, during severe hypoxia or anoxia, the cell initiates a cascade of events that leads to apoptotic cell death, thereby preventing the accumulation of cells with hypoxia induced mutations. (bmj.com)

Endothelin-1 (ET-1), a peptide product of endothelial cells, is mitogenic for fibroblasts and smooth muscle cells. (johnshopkins.edu)

Cultured human arterial endothelial cells (HAECs) were exposed to hypoxia/deoxygenation (H/R). Cells were also treated with baicalin at serially diluted concentrations. (medscimonit.com)
A recent study provided evidence that pyroptosis is involved in the inflammatory process of human pulmonary artery smooth muscle cells (HPASMCs) in HPH (Zhang et al. (biomedcentral.com)
Here we provide evidence for what we believe is a novel mechanism regulating HIF-1α levels in isolated human ECs during hypoxia. (umn.edu)
Hypoxia stimulated SRF/MYOCD expression in human cerebral VSMCs and in animal models of AD. (nature.com)
Figure 7: Hypoxia increases MYOCD and SRF expression in human cerebral VSMC and pial vessels in APPsw ± mice. (nature.com)
RESEARCH DESIGN AND METHODS- Human microvascular HMEC-1 endothelial cells were incubated in low and high glucose concentrations (5 and 30 mmol/l, respectively), and activation of nrf2 was assessed by nuclear translocation. (diabetesjournals.org)
Figure 1: We use human ADPKD-derived cells which grow in three-dimensional organotypic cultures. (sheffield.ac.uk)

Flow cytometry was used to detect cell apoptosis. (medscimonit.com)
PKCδ-siRNA transfection increased cell viabilities and reduced cell apoptosis in HAECs exposed to H/R. Baicalin treatment preserved cell viabilities and reduced apoptosis of H/R-exposed HAECs in a concentration- dependent manner. (medscimonit.com)
Baicalin treatment reduced phosphorylation levels of PKCδ and p53, as well as the expression levels of active caspase3 and bax in HAECs exposed to H/R. The treatment of PKC activator PEP005 impaired the protective effects of baicalin in increasing cell viabilities and reducing apoptosis in HAECs exposed to H/R. (medscimonit.com)
Baicalin exerts vascular a protective effect on HAECs exposed to H/R by reducing cell apoptosis. (medscimonit.com)
Studies have shown that endothelial cells (ECs) are the direct targets of hypoxia and are involved in the pathogenesis process of HPH leading to cell hyperproliferation, inhibition of apoptosis and plexiform intima injuries (Ranchoux et al. (biomedcentral.com)
Based on the hypothesis that alpha6beta1 is necessary for the survival of these cells in the tumor microenvironment, we report here that hypoxia protects these cells from apoptosis induced by serum deprivation and that hypoxia-mediated protection requires alpha6beta1 expression. (umassmed.edu)
EGF may induce a limited number of neuronal cells because it also enhanced apoptosis. (umassmed.edu)
During hypoxia, an intricate balance exists between factors that induce or counteract apoptosis, or even stimulate proliferation. (bmj.com)
Understanding the regulation of apoptosis during hypoxia and the mechanisms of resistance to apoptosis might lead to more specific treatments for solid tumours. (bmj.com)

In fact, in addition to their potent vasodilating effect, EETs have potent anti-inflammatory properties, inhibit platelet aggregation, promote fibrinolysis, and reduce vascular smooth muscle cell proliferation. (frontiersin.org)
Finally to appraise how tissue function might be affected by endothelial cell hypoxia the contractility of vascular smooth muscle was examined. (bl.uk)

Hypoxia leads to the up-regulation of hypoxia-induced transcription factors (HIF)-a and HIF-2a, which are the master regulators of proangiogenic signals, mainly the vascular endothelial cell growth factors (VEGFs). (medscape.com)

Our data provide evidence that estrogen signaling mediates the tumor vasculogenic process required for breast cancer progression and involves a key regulator of the hypoxia signaling pathway. (biomedcentral.com)
Hypoxia triggers a proangiogenic pathway involving cancer cell microvesicles and PAR-2-mediated heparin-binding EGF signaling in endothelial cells. (lu.se)

These steps are supported by functions of the cancer cells themselves or of the tumor microenvironment. (medscape.com)

A hypoxia-induced mouse model of pulmonary hypertension (PH) was used to test preventive effect of conserved fragment TFO2 of FENDRR. (biomedcentral.com)

Endothelial cell lineage tracing showed that BNP directly stimulated the proliferation of resident endothelial cells via NPR-A binding and p38 MAP kinase activation. (elifesciences.org)

Hypoxia has been shown to alter several biochemical mechanisms in endothelial cells. (usp.br)

American Type Culture Collection [ATCC], Manassas, VA) were cultured in minimal essential medium (Thermoscientific, Logan, UT) and the media was supplemented with 10% fetal bovine serum (Invitrogen Corp., Carlsbad, CA), 100 U/ml penicillin, and 100 µg/ml of streptomycin (Invitrogen Corp.). Cells were maintained at 37 °C in logarithmic scale in a 75 cm 2 cell culture flask. (molvis.org)
In this study we examined the effect of hypoxia on ET-1 production by bovine pulmonary vascular endothelial cells. (johnshopkins.edu)

Methods Cell. (bioind.com)

FENDRR overexpression inhibited hypoxia-induced HPAEC pyroptosis. (biomedcentral.com)

The metastatic process involves sequential steps, including invasion through the surrounding extracellular matrix (ECM) and stromal cell layers, intravasation into the lumina of blood vessels, and survival in the circulation. (medscape.com)
Direct invasion by carcinoma cells of the stromal compartment involves active proteolysis effected principally by matrix metalloproteinases (MMPs), while degrading the BM and other ECM that lie in the path of invading tumor cells, MMP-expressing cells also liberate growth factors that are sequestered there, thereby fostering cancer cell proliferation. (medscape.com)

BBA - Molecular Cell Research , 1134 (2), 105-111. (johnshopkins.edu)
The 14 C incorporation and specific activity (disintegrations per minute per nanomole) of three major arachidonyl molecular species (16:0-20:4, 18:120:4, and 18:0-20:4) of each phospholipid class were determined in cells chased under either of the two conditions. (elsevierpure.com)
The most commonly studied isoforms are VEGF121, VEGF165, and VEGF189 (f, d and b, respectively) All seven isoforms differ in their molecular mass and in biological properties such as their ability to bind to cell-surface heparan-sulfate proteoglycans. (sigmaaldrich.com)
Despite the positive molecular adaptations observed after various IHT modalities, the characteristics of optimal training stimulus in hypoxia are still unclear and their functional translation in terms of whole-body performance enhancement is minimal. (bmj.com)
A broad understanding of the sequence of events, cells involved, relative time table, and molecular signaling can allow for maximum optimization of this important patient care issue. (medscape.com)
Although seemingly basic in concept, advances in molecular science have allowed modern medicine to gain a true appreciation of the complex interplay between the cells involved in the phases of wound healing. (medscape.com)
This process is marked by a complex and coordinated set of molecular changes leading to the motile behavior of the invading cancer cells, which involves dynamic cytoskeletal changes, cell-matrix interactions, localized proteolysis, actin-myosin contractions, and focal contact disassembly. (medscape.com)
Correspondence with low molecular weight chemicals that results in the priming of chemical-specific, Prof. Dr. Stefan F. Martin, Allergy Research skin-homing CD8+ Tc1/Tc17 and CD4+ Th1/Th17 cells. (cdc.gov)

Twelve normotensive (38â ±â 10 years) and nine hypertensive men (45â ±â 11 years) were exposed to 5-min trials of normoxia (21% O2) and isocapnic hypoxia (10% O2). (bvsalud.org)
2 and IL-8 during hypoxia compared to normoxia. (lu.se)

Pyroptosis, a novel form of proinflammatory programmed cell death, is mainly dependent on the activation of caspase-1 or caspase-11 in inflammasomes (Shi et al. (biomedcentral.com)
Hypoxia-induced miR-424 was regulated by PU.1-dependent transactivation. (umn.edu)
BIO-MPM-1 Medium is a multi-purpose, serum-free medium for culture of a wide variety of anchorage-dependent cells. (bioind.com)

The severity of hypoxia determines whether cells become apoptotic or adapt to hypoxia and survive. (bmj.com)

On the other hand, the number of circulating hEPC is increased in hypoxia or acute myocardial infarction (AMI). (intechopen.com)
The association of functional polymorphisms in genes expressed in endothelial cells and smooth muscle cells with the myocardial infarction. (cdc.gov)

As greater understanding of the growth factors involved in wound healing emerges, future patient care may include scarless wound healing and transplant of tissues engineered from stem cell progenitors. (medscape.com)

Cells will adapt by activating the expression of genes involved in metabolic adaptation, such as those involved in glycolysis. (bmj.com)

Single-cell motility is spatially heterogeneous and driven by metabolic energy. (nature.com)
Here, we use single-cell metabolic imaging to measure glycolysis in individual endothelial cells with genetically encoded biosensors capable of deciphering metabolic heterogeneity at subcellular resolution. (nature.com)
In addition, the number and the migratory activity of these cells are inversely correlated with risk factors such as hypertension, hypercholesterolemia, diabetes, and metabolic syndrome. (intechopen.com)
Alterations in cell viability and metabolic activity were determined. (lu.se)
Metabolic activity was significantly increased by hypoxia in both epithelial cell types. (lu.se)

Breast cancer stem cells (BCSCs) play a critical role in tumor recurrence and metastasis. (nih.gov)
Metastases represent the end products of a multistep cell-biological process termed the invasion metastasis cascade, which involves dissemination of cancer cells to anatomically distant organ sites. (medscape.com)
Infiltrated cells might proceed towards overt metastasis with or without an intervening period of latency (dormancy). (medscape.com)

Furthermore, because VEGFA-165 expression is hypoxia sensitive, it may play a role in respiratory plasticity after prolonged exposures to low oxygen. (jneurosci.org)
For investigation of RGS5, we hypothesize that silencing RGS5 expression using siRNA technology (5) leads to secretome changes that effect cells at the BBB. (lu.se)

Coordinated activation of RhoA and RhoB maximized the hypoxia-induced stress fiber formation caused by RhoB/mammalian homolog of Drosophila diaphanous-induced actin polymerization and RhoA/Rho kinase-induced phosphorylation of myosin light chain on Ser19. (brighton.ac.uk)
Mammalian cells have developed a range of adaptations to survive acute and even prolonged hypoxia. (bmj.com)

The formulation contains no albumin, which has been found to be non-essential for cell growth, and even prevents efficient adhesion in some cases. (bioind.com)

Hypoxia-driven pulmonary vascular remodelling is one of the underlying mechanisms, although many other mechanisms may contribute [ 5 ]. (ersjournals.com)

RhoA activation triggers a glycolytic burst through the translocation of the glucose transporter SLC2A3/GLUT3 to fuel the cellular contractile machinery, as demonstrated across multiple endothelial cell types. (nature.com)

Anatomy10

Organisms1

Diseases4

Chemicals and Drugs11

Phenomena and Processes6

Angiogenesis13

Hypoxic18

Response to hypoxia5

VEGF18

Microvascular endothelial cells1

Induces4

Progenitor cells2

Inducible5

Respond to hypoxia2

Abstract1

Mature endothelial cells1

Periods of hypoxia2

Damage endothelial2

Dysfunction3

Factor26

Endothelium2

Migration6

Acute hypoxia2

Tissue hypoxia2

Pericytes4

Angiogenic1

Stimulates2

Vitro3

Exposure3

Epithelial cells2

Protein2

Inflammation1

Intermittent2

Metabolism4

Promotes1

Initiates2

Fibroblasts1

Human7

Apoptosis9

Vascular smooth2

Regulation1

Pathway2

Tumor microenvironment1

Pulmonary hypertension1

Stimulated the proliferation1

Biochemical1

Bovine2

Methods1

Overexpression1

Stromal2

Molecular8

Normoxia2

Dependent3

Apoptotic1

Myocardial infarction2

Stem cell1

Genes1

Metabolic5

Metastasis3

Expression2

Mammalian2

Adhesion1

Mechanisms1

Activation1