• The enhancing effect of ECM on BCE cell growth and culture longevity clearly illustrates the importance of the cell substrate in the control of proliferation of these cells. (nih.gov)
  • In this study, in attempt to provide fresh information about the impact of CS on proliferation of EPCs to elucidate the pathophysiological mechanisms of the diseases related to CS in cellular level, we assessed the proliferation of EPCs after interfering the cells with a series of concentrations of CSE for various times of exposure in vitro . (hindawi.com)
  • Post-facto analysis was performed on dissected vessels using confocal microscopy techniques, live/dead staining, and polymerase chain reaction (PCR) testing to probe cellular growth and proliferation indicators. (aps.org)
  • Our results further indicate that MCT4 promotes proliferation and survival by altered cell cycle regulation and cell death mechanisms. (hindawi.com)
  • For this purpose, we designed an MCT4 overexpression and knockdown/inhibition model to explore the functional consequences of MCT4 expression on cancer cell metabolism, survival, proliferation, migration, invasion potential, and angiogenesis as well as on cell cycle profiles and cell death mechanisms. (hindawi.com)
  • Endothelial cell lineage tracing showed that BNP directly stimulated the proliferation of resident endothelial cells via NPR-A binding and p38 MAP kinase activation. (elifesciences.org)
  • BNP also stimulated the proliferation of WT1 + epicardium-derived cells but only in the hypoxic area of infarcted hearts. (elifesciences.org)
  • The origin of new endothelial cells (i.e. resident or infiltrating) as well as the underlying mechanism leading to their proliferation (partial endothelial-to-mesenchymal transition [EndMT] or not) have long been debated. (elifesciences.org)
  • Furthermore, we demonstrated that these automatically isolated cells bear proliferation and differentiation capacities comparable to manually isolated cells in vitro. (biomedcentral.com)
  • Another example is the growth of cancerous solid tumours, which are dependent on the proliferation of new blood vessels. (ucl.ac.uk)
  • Hamilton: B. C. Decker Inc.). A number of anti-angiogenic activities have been reported for this protein, such as inhibition of endothelial cell proliferation, migration, and tube formation. (justia.com)
  • Pathway-directed microarrays revealed increased transcription of insulin-like growth factor I (IGF-1), a modulator of cell growth and proliferation upstream of mTOR. (lonza.com)
  • If you seed too high, the cells will attach but there is insufficient room for further proliferation and they will stop replicating. (mbpinc.net)
  • Therefore, the MAPK family governs almost all the physiological functions and processes such as cell adaptation, proliferation, differentiation, survival, and programmed cell death. (spandidos-publications.com)
  • In vitro, PDGF appears to enhance cell proliferation of meningioma cells. (medscape.com)
  • Although bone marrow-derived cells do appear to localize to injured vessels and promote an angiogenic switch, other studies have suggested these cells do not contribute directly to the functional endothelium, instead acting via paracrine methods to provide support for the resident endothelial cells. (wikipedia.org)
  • Low-serum cell culture medium for endothelial cells from large blood vessels. (promocell.com)
  • Endothelial progenitor cell (or EPC) is a term that has been applied to multiple different cell types that play roles in the regeneration of the endothelial lining of blood vessels. (wikipedia.org)
  • This process enables tumor cells, including those within the core of the heterogeneous tumor mass to survive, despite being at a distance from blood vessels that supply their metabolic needs [ 16 , 17 ]. (hindawi.com)
  • The discovery of a protein that encourages blood vessel growth, and especially 'bad' blood vessels - the kind that characterise diseases as diverse as cancer, age-related macular degeneration and rheumatoid arthritis - has been reported in the journal Nature. (ucl.ac.uk)
  • TGF-beta regulates both the maintenance of normal healthy blood vessels, and the unwanted growth of harmful blood vessels, but precisely how it promotes two opposing outcomes is a biological paradox. (ucl.ac.uk)
  • This causes a switch in TGF-beta signalling away from a normal vessel maintenance pathway towards a pathway that promotes the growth of new harmful blood vessels. (ucl.ac.uk)
  • As a vascular biologist and vascular surgeon, Dr. Greisler has worked extensively with fibroblast growth factor (FGF)-1, particularly within a fibrin delivery system for the purpose of stimulating angiogenic mechanisms to re-endothelialize blood vessels. (dermatologytimes.com)
  • Endothelial cells lining the inner surface of blood vessels and lymphatic vessels play an indispensable role in vascular homeostasis. (bvsalud.org)
  • The hemostatic system consists of platelets, coagulation factors, and the endothelial cells lining the blood vessels. (medscape.com)
  • These computer chip-sized devices provide cells with a more physiological 3D environment that supports cell differentiation, enabling organ-like functions. (nature.com)
  • The goal is to characterize the dynamic flow conditions which drive endothelial cell mechanotransduction, cellular growth, and morphological maturation, as well as the differentiation of ADSCs into endothelial-like phenotypes. (aps.org)
  • One complementary therapy could be 're-activating' vasculogenesis (i.e. the differentiation of precursor cells into mature endothelial cells), a mechanism that occurs in the heart during development but is quiescent in adult hearts. (elifesciences.org)
  • Recently, several clinical trials showed an increased left ventricular ejection fraction (LVEF) and an improved regional perfusion following intramyocardial application of cluster of differentiation (CD) 133 + stem cells [ 5 - 9 ]. (biomedcentral.com)
  • Differentiation was compared to mesenchymal stem cells derived from human bone marrow, which is known to differentiate into adipocyte, osteocyte, and chondrocyte lineages. (jove.com)
  • In this study, we explore the role of HMGB1 in advanced glycation end products (AGEs)-induced vascular endothelial growth factor A (VEGF-A) production in rat retinal ganglion cell line 5 (RGC-5) cells. (molvis.org)
  • The VEGF-A protein and mRNA levels in conditioned medium of RGC-5 cells incubated with AGE-modified BSA (AGE-BSA) were examined with real-time PCR and enzyme-linked immunosorbent assay (ELISA), and BSA-treated cells were used as controls. (molvis.org)
  • Compared with the BSA controls, the RGC-5 cells incubated with AGE-BSA showed a dose- and time-dependent increase in VEGF-A mRNA and VEGF-A protein secretion in the supernatant, with the highest levels achieved at 24 h. (molvis.org)
  • HMGB1 is implicated in the production of VEGF-A in retinal ganglion cell line-5 (RGC-5). (molvis.org)
  • In previous studies, many signalling molecules have been identified that control angiogenesis, with the secreted protein vascular endothelial growth factor (VEGF) being considered as the master regulator. (ucl.ac.uk)
  • Endostatin also suppresses vascular endothelial growth factor (VEGF)-induced vascular permeability (Takahashi et al. (justia.com)
  • Activated SIRT1 signals hypoxia inducible factor (HIF)-2α, which transactivates vascular endothelial growth factor (VEGF) and erythropoietin. (molvis.org)
  • However, its role has not been evaluated in hypoxic choroidal endothelial cells (CECs), the precursor of VEGF-mediated choroidal angiogenesis. (molvis.org)
  • The study focused on the determination of vascular endothelial growth factor VEGF and interleukins IL-8, IL-10 and IL-12. (helsinki.fi)
  • Furthermore, it was revealed that treatment with vitexin induced hypoxia inducible factor 1α subunit (HIF‑1α) and vascular endothelial growth factor (VEGF) protein expression, and suppressed phosphorylated‑p38 MAP kinase (p38) protein expression in sevoflurane‑induced newborn rat. (spandidos-publications.com)
  • VEGF is purified from bovine pituitary follicular cells in 1959 and is a vascular endothelial cell-specific mitogen that promotes the growth of vascular endothelial cells. (spandidos-publications.com)
  • In addition, the addition of VEGF to the culture medium can increase the survival rate of neurons in the cerebral cortex as well as the number and length of ganglion cell processes, to promote nerve growth. (spandidos-publications.com)
  • Inhibition of neutrophil proteases inhibited neutrophil transmigration on geltrex mix, but not collagen I. These findings highlight the important role of the ECM in determining cell phenotype and response to inhibitors. (nature.com)
  • Conversely, inhibition of LRG1 in mouse models reduces the harmful blood vessel growth associated with retinal disease. (ucl.ac.uk)
  • Professor John Greenwood, senior author of the research from the UCL Institute of Ophthalmology said: "We have discovered that a secreted protein, LRG1, promotes new blood vessel growth and its inhibition prevents pathological blood vessel growth in ocular disease. (ucl.ac.uk)
  • Inhibition of IGF-1R prevents ionizing radiation-induced primary endothelial cell senescence. (lonza.com)
  • Studies of signal transduction pathways leading to accelerated senescence have revealed that inhibition of mammalian target of rapamycin (mTOR) by rapamycin rescues cells from senescence. (lonza.com)
  • 1 - 4 Multiple mechanisms may be involved in its potent antitumor effect, including induction of apoptosis, cell cycle arrest, telomerase inhibition, antiangiogenesis activity, and an antimetastasis effect. (dovepress.com)
  • particularly, the effect of miR-195-5p modulation seemed to affect cell cycle, while miR-107 seemed to be implicated in the inhibition of cells migration. (biomedcentral.com)
  • The neutralizing antibody response to PC-positive DBs was superior to that of PC-negative DBs, as tested on fibroblasts, epithelial cells, and endothelial cells and for both animal species used. (asm.org)
  • Heparin binding growth associated molecule (pleiotrophin or HBGAM), for example, interacts with the low-affinity heparan sulfate proteoglycans, which are much more abundant on endothelial cells than on smooth muscle cells or fibroblasts. (dermatologytimes.com)
  • Endothelial colony forming cells represent a distinct population that has been found to have the potential to differentiate and promote vessel repair. (wikipedia.org)
  • Hemangioblasts (or their in vitro counterpart, blast-colony forming cells) are cells believed to give rise to both the endothelial and hematopoietic systems during early development. (wikipedia.org)
  • During an inflammatory response, neutrophils move from the blood to the site of inflammation by transmigrating across the endothelial barrier and through the basement membrane (BM) of the surrounding extracellular matrix (ECM). (nature.com)
  • Toll-like receptors (TLRs) enable innate immune cells, such as macrophages, to recognize a wide variety of microbial ligands, thereby promoting inflammation. (frontiersin.org)
  • Overall, the findings suggested that S/V could downregulate the expression of MALAT1, inhibit inflammation and apoptosis and improve endothelial function in ox‑LDL‑induced HUVECs via inactivating the TLR4/NF‑κB signaling pathway. (spandidos-publications.com)
  • Vascular endothelial cells serve a key role in the pathogenesis of atherosclerosis by regulating vascular tension, platelet adhesion, inflammation and fibrinolysis ( 5 ). (spandidos-publications.com)
  • accepted 20 January 2014) Increasing evidence has demonstrated that beyond its role in activation of protein C, endothelial cell protein C receptor (EPCR) is involved in vascular inflammation. (sagepub.com)
  • Fetal haemoglobin (HbF) induction is the primary treatment response, along with improved anaemia, reduced haemolysis, myelosuppression and decreased endothelial inflammation. (cdc.gov)
  • Human renal epithelial cells (HREpCs) (PromoCell) were cultured in renal epithelial cell growth medium-2 (PromoCell). (biomedcentral.com)
  • ECFCs are isolated by plating peripheral blood mononuclear fraction on collagen-coated plates, removing non-adherent cells, and culturing for weeks until the emergence of colonies with a distinctive cobblestone morphology. (wikipedia.org)
  • The cell composition consists of a homogenous population of intestinal cells and are characterized by viability, morphology, plating efficiency along with cell characterization by staining for CK8 and CK18. (novabiosis.com)
  • Primary Human Saphenous Vein Endothelial Cells isolated from saphenous veins from single donors. (promocell.com)
  • DetachKit for gentle and effective detachment of adherent primary human cells. (promocell.com)
  • In vitro investigation of human umbilical vein endothelial cell (HUVEC) and adipose-derived stem cell (ADSC) responses in a biomimetic 3D bioprinted blood vessel model under complex physiological flows was performed. (aps.org)
  • In vitro studies revealed similar cell permissiveness and replication of CSUR381 (compared with 2 other NiV isolates) in both bat and human cell lines. (cdc.gov)
  • According to the European Medicines Agency (EMA) BM-derived CD133 + stem cells intended for the regeneration of human ischemic heart tissue are classified as an advanced therapy medicinal product (ATMP) [ 17 ]. (biomedcentral.com)
  • We predict, therefore, that abnormal blood vessel growth is also a conserved process and that the role of LRG1 is equally applicable to human pathological angiogenesis. (ucl.ac.uk)
  • We analyzed the cellular consequences of hantavirus infection by measuring adhesion and migration capacity of human renal cells infected with Puumala (PUUV) or Hantaan (HTNV) virus. (biomedcentral.com)
  • However, the underlying mechanisms and effects of hantavirus infection on human renal cells have not been investigated so far. (biomedcentral.com)
  • To identify the consequences of hantavirus infection, we analyzed adhesion and motility capacity of human renal cells infected with hantaviruses PUUV or HTNV. (biomedcentral.com)
  • The human podocyte cell line was derived from human normal podocytes conditionally transformed with a temperature-sensitive mutant of the simian virus 40 (SV40) large T antigen. (biomedcentral.com)
  • In the present study, we demonstrate that following exposure to plasma from diabetic patients, the human promonocytic cell line U937 exhibits a significant elevation in core 2 GlcNAc-T activity and increased adherence to cultured retinal capillary endothelial cells. (diabetesjournals.org)
  • Cryopreserved human intestinal cells are viable for one year when stored under these conditions. (novabiosis.com)
  • Cryopreserved primary human intestinal cells are assured to be viable and functional when thawed and maintained properly. (novabiosis.com)
  • Novabiosis primary human intestinal epithelial cells are specifically critical in the digestion and retention of nutrients and shields the organism from toxins and bacteria found in the intestine. (novabiosis.com)
  • Explore our extensive collection of Human and Animal Cells, Clinical and Patient-Derived models. (ixcellsbiotech.com)
  • The goal of this protocol is to test the ability of progenitor cells derived from human perivascular adipose tissue to differentiate into multiple cell lineages. (jove.com)
  • This protocol describes a simple and an economical method for isolation of endothelial cells from human umbilical vein. (bvsalud.org)
  • Thus, umbilical vein is an ideal source for isolation of endothelial cells of human origin. (bvsalud.org)
  • Aim of this study was to evaluate possible in vitro cytotoxic effects of two new-generation antiepileptic drugs on a human glioma cell line U87MG. (biomedcentral.com)
  • Description: The WM59 monoclonal antibody reacts with human CD31, also known as platelet-endothelial cell adhesion molecule-1 (PECAM-1) and gpIIa. (thermofisher.com)
  • Applications Tested: The WM-59 (WM59) antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. (thermofisher.com)
  • This study aimed to explore the effects of chemerin on the functions of human endothelial progenitor cells (EPCs) and to investigate its role in lipid accumulation in ApoE-knockout (ApoE−/−) mice. (biomedcentral.com)
  • 2. The aim of this work was to investigate how hypoxia affects human lung epithelial cells in combination with profibrotic stimuli and its correlation to pathogenesis. (lu.se)
  • 2005). Notch1 and syndecan-1 potent human embryonic stem (ES) cells. (lu.se)
  • CD133+), but are rarely codetected with the neural stem dents, very few human-specific NSC markers have been cell (NSC) marker CD15. (lu.se)
  • To investigate the putative anti-proliferative effect, apoptosis assay, microRNA expression profile and study of cell cycle were performed. (biomedcentral.com)
  • CD31 is a multifunctional molecule with diverse roles in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, negative regulation of immunoreceptor signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. (thermofisher.com)
  • We characterize the role of increased Lyn kinase activity in neutrophils, endothelial cells and lesional liver biopsies and utilized an iPSC-derived endothelial cell platform for disease modeling of neutrophilic vasculitis and to screen and evaluate drug efficacy. (nature.com)
  • While some other authors have contested these, and maintained that they are true EPCs, many investigators have begun to term these cells colony forming unit-Hill cells (CFU-Hill) or circulating angiogenic cells (CAC) instead (depending on the method of isolation), highlighting their role as hematopoietic myeloid cells involved in promoting new vessel growth. (wikipedia.org)
  • By method of isolation and cell function, three main populations of putative adult EPCs have been described. (wikipedia.org)
  • Certain developmental cells may be similar to or the same as other endothelial progenitors, though not typically referred to as EPCs. (wikipedia.org)
  • Endothelial progenitor cells (EPCs) contribute to neovascularization and play an important role in the development of these diseases. (hindawi.com)
  • The bone-marrow-derived endothelial progenitor cells (EPCs) provide an alternative source of endothelial cells (ECs) that contributes to neovessel formation in endothelium structure [ 4 , 5 ]. (hindawi.com)
  • Angioblasts are believed to be a form of early progenitor or stem cell which gives rise to the endothelium alone. (wikipedia.org)
  • In vitro studies have reported a dramatic increase in PC anticoagulant pathway is the endothelial cell protein C EPCR shedding from the endothelium by a wide variety receptor (EPCR). (sagepub.com)
  • Diabetes causes retinal microvasculopathy associated with pericyte cell death, microaneurysms, abnormal vascular permeability, and macular edema. (molvis.org)
  • The exact pathogenic mechanism by which capillary nonperfusion, as demonstrated by fluorescein angiography, occurs is still unclear, but recent experimental animal studies ( 3 - 5 ) demonstrate that increased leukocyte entrapment in retinal capillaries of diabetic rats is an early event associated with areas of capillary nonperfusion and the development of endothelial cell damage. (diabetesjournals.org)
  • We recently reported that raised activity of the glycosylating enzyme core 2 β 1,6- N -acetylglucosaminyltransferase (GlcNAc-T) through protein kinase C (PKC)β2-dependent phosphorylation plays a fundamental role in increased leukocyte-endothelial cell adhesion and capillary occlusion in retinopathy. (diabetesjournals.org)
  • These effects of diabetic plasma on enzyme activity and cell adhesion, mediated by PKCβ2-dependent phosphorylation of the core 2 GlcNAc-T protein, were found to be triggered by increased plasma levels of tumor necrosis factor (TNF)-α. (diabetesjournals.org)
  • Endostatin inhibits endothelial cell migration by inhibiting phosphorylation of focal adhesion kinase via binding to α5β1 integrin (Wickstrom et al. (justia.com)
  • Furthermore, employing flow cytometry, Western blotting and adhesion assays for PBMCs and Jurkat-T cells, we elucidate that insulin enhances fibronectin adherence of quiescent lymphocytes through non-canonical signalling involving insulin-like growth factor-1 receptor (IGF-1R) autophosphorylation, phospholipase C gamma-1 (PLCγ-1) Tyr783 phosphorylation and inside-out activation of ß-integrins respectively. (bvsalud.org)
  • CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling by a dual immunoreceptor tyrosine-based inhibitory motif (ITIM) that upon associated kinases-mediated phosphorylation provide docking sites for protein-tyrosine phosphatases. (thermofisher.com)
  • 2000). In addition, phosphorylation of endothelial cell surface, where it enhances the rate of PC p38MAPK, ERK1/2, and JNK was increased by stimula- activation (Mosnier et al. (sagepub.com)
  • Low density BCE cell cultures maintained on extracellular matrix (ECM)-coated dishes and plated in the total absence of serum proliferate actively when exposed to a synthetic medium supplemented with high density lipoprotein (HDL 500 micrograms protein/ml), transferrin (10 micrograms/ml), insulin (5 micrograms/ml), and fibroblast (FGF) or epidermal growth factor (EGF) added at concentrations of 100 or 50 ng/ml, respectively. (nih.gov)
  • Use of PriCoat TM T25 Flasks ( G299 ) or Applied Cell Extracellular Matrix ( G422 ) is required for cell adhesion to the culture vessels. (mbpinc.net)
  • This is followed by their culture on extracellular matrix (ECM) protein coated tissue culture flasks in presence of endothelial cell growth medium. (bvsalud.org)
  • Unfortunately, there are no unique markers for endothelial progenitors that are not shared with other endothelial or hematopoietic cells, which has contributed to the historical controversy surrounding the field. (wikipedia.org)
  • This multi-step process is enabled by inflammatory receptors and molecules expressed by the endothelial cells which capture the neutrophils and support their extravasation, alongside gradients of chemokines that guide the neutrophils through the ECM. (nature.com)
  • In addition, the presence of EVs reduced inflammatory responses in Pam 3 CSK 4 -treated endothelial cells and HEK Dual reporter cells, demonstrating that TLR2-EVs can act as decoy receptors. (frontiersin.org)
  • Once implanted, these tissues require that one induce vascularization in vivo through means such as inflammatory mechanisms and induction of growth factor expression. (dermatologytimes.com)
  • Schisandra chinensis -lignans inhibited especially the release of nitric oxide in both C. pneumoniae -infected and LPS-stimulated cells which may tell about their broad anti-inflammatory properties. (helsinki.fi)
  • Chemerin is an adipocytokine involved in the recruitment of immune cells [ 11 ] and serves as a bridge between various immune cells from the initial stage of inflammatory diseases through the development process [ 12 ], and its receptor CMKLR1 (also named ChemR23 in humans) is a specific functional member of the G protein receptor family. (biomedcentral.com)
  • Endothelial progenitor cells are likely important in tumour growth and are thought to be critical for metastasis and the angiogenesis. (wikipedia.org)
  • However angiogenesis also plays a major role in many diseases where new vessel growth can be harmful. (ucl.ac.uk)
  • The mechanism through which LRG1 promotes angiogenesis is by modifying the signalling of a multifunctional secreted growth factor called transforming growth factor beta (TGF-beta). (ucl.ac.uk)
  • Here, gain- and loss-of-function assays demonstrated that miR-874 inhibited the tumor angiogenesis of GC cells in vitro and in vivo . (oncotarget.com)
  • Distinct hallmarks of cancer were examined using in silico analysis, various in vitro cell culture assays, and ex vivo organotypic rat brain slice culture model. (hindawi.com)
  • We investigated the impact on tumor malignancy in F98 glioma cells using in silico analysis, in vitro cell culture assays, and ex vivo vascular organotypic glioma impact model (VOGIM) [ 35 ] by implanting F98 cells into rat brain slices. (hindawi.com)
  • Following verification of a stem-like signature (upregulated expression of stemness markers, resistance to chemotherapy, increased spheroid formation, and tumorigenesis capabilities despite inoculation of a small number of cells), we analyzed the interaction of these cells with macrophages via direct and indirect coculture assays. (springer.com)
  • The biological activity of automatically isolated CD133 + cells was evaluated and compared to manually isolated CD133 + cells via functional assays as well as immunofluorescence microscopy. (biomedcentral.com)
  • More recently, mesoangioblasts have been theorized as a cell giving rise to multiple mesodermal tissues. (wikipedia.org)
  • Heterotopic ossification (HO) is the abnormal growth of bone in soft connective tissues that occurs as a frequent complication in individuals with traumatic brain injury (TBI) and in rare genetic disorders. (mdpi.com)
  • The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
  • Apart from regulating vessel tone and forming an anti-thrombotic and anti-atherosclerotic surface, the dynamic endothelial barrier controls transport of solutes and fluid in and out of tissues at the capillary bed. (bvsalud.org)
  • pathomechanisms that regulate extravasation of damaging immune cells into surrounding tissues are poorly understood. (nature.com)
  • Cryopreserved: Cryogenic vial containing 500.000 viable cells. (promocell.com)
  • Viability, Cells per vial, Coverage by Crystal Violet at day 7, Cytokeratin 8 + 18 immunostaining. (novabiosis.com)
  • The frozen vial of cells will be shipped in Dry ice while T25 flasks by themselves are shipped at room temperature. (mbpinc.net)
  • In most cases, a vial of primary cells shipped in dry ice (-70°C) can be placed back into liquid nitrogen and recovered at a later date by rapid thawing. (mbpinc.net)
  • How many cells are each vial? (mbpinc.net)
  • The number of cells in a vial is lot-dependent. (mbpinc.net)
  • A Certificate of Analysis stating the cell quantity of the vial will be provided with your order. (mbpinc.net)
  • Endothelial pipe formation assay Subconfluent HUVECs had been gathered and resuspended in restricting moderate (Moderate 200 with 0.2% LSGS) and treated using the indicated focus of exosomes. (exposed-skin-care.net)
  • HUVECs seeded on Matrigel-coated chamber slides (Thermo Scientific Inc. Rochester NY) had been incubated at 37°C with tagged exosomes at a focus of 1 1 μg exosomes/10 0 cells or as described. (exposed-skin-care.net)
  • Where indicated two labeled populations of HUVECs were generated after incubation of the cells in UF010 standard culture monolayer for 3 h with PKH26- or PKH67-labeled exosomes. (exposed-skin-care.net)
  • The regeneration potential of these cells is mainly based on their direct contribution to neovascularization and on their secretion of various paracrine factors activating pro-angiogenic mechanisms [ 13 - 16 ]. (biomedcentral.com)
  • miRNA-132 acts as an angiogenic switch by suppressing endothelial p120RasGAP expression, leading to Ras activation and the induction of neovascularization [ 9 ]. (oncotarget.com)
  • It upregulates angiogenic growth factors and promotes neovascularization. (molvis.org)
  • Further, GA-mPEG 2000 micelles were effective in inhibiting tumor growth and prolonged survival in subcutaneous B16-F10 and C26 tumor models. (dovepress.com)
  • 1983) and the multipotent progenitor cells from fetal disease (Bjorklund and Lindvall, 2000). (lu.se)
  • 2000). In *Correspondence to: Perrine Barraud, Department of Veterinary Medi- contrast, several cell surface markers have been used to cine, Neurosciences, Madingley Road, Cambridge CB3 OES, United enrich for NSCs in the rodent CNS. (lu.se)
  • Functional studies revealed increased expression of ICAM-1 on induced patient-derived endothelial cells (iECs) and of β2-integrins on patient neutrophils that increase neutrophil adhesion and vascular transendothelial migration (TEM). (nature.com)
  • Tissue infiltration by activated innate immune cells, including monocytes, macrophages, and neutrophils, if untreated, leads to rapidly progressive organ injury, that can involve many organ systems. (nature.com)
  • The entire manufacturing process, the examination of cell number and viability of the final CP as well as the depletion of non-target cells were part of the validation process, which was performed in compliance with EU guidelines for GMP. (biomedcentral.com)
  • Cell Counting Kit‑8 assay was used to detect cell viability. (spandidos-publications.com)
  • Compared with the ox‑LDL group, S/V treatment significantly increased the cell viability, NO concentration and Bcl‑2 expression, decreased the levels of IL‑1β, IL‑6 and TNF‑α and reduced the expressions of MALAT1, ICAM‑1, VCAM‑1, cleaved‑caspase‑3, Bax, TLR4 and p‑p65. (spandidos-publications.com)
  • However it is important to note that the viability of some sensitive cell types may be reduced by the temperature shift of such treatment, making recovery more difficult. (mbpinc.net)
  • ABM does not warrant the viability of cells stored at -70C after shipments have been received. (mbpinc.net)
  • In addition some of the compounds were tested for the cell viability using resazurin assay. (helsinki.fi)
  • Alterations in cell viability and metabolic activity were determined. (lu.se)
  • Molecular genetic analysis of early outgrowth putative EPC populations suggests they do indeed have monocyte-like expression patterns, and support the existence of a separate population of progenitors, the late outgrowth, or endothelial colony forming cell (ECFC). (wikipedia.org)
  • Furthermore, early outgrowth cells maintain other monocyte functions such as high Dil-Ac-LDL and India ink uptake and low eNOS expression. (wikipedia.org)
  • Stable F98 glioma cell lines with MCT4 overexpression, normal expression, and knockdown were generated. (hindawi.com)
  • Previous studies have shown that the expression of MALAT1 is increased in endothelial cells induced by ox-LDL and its expression level can reflect the damage degree of endothelial cells ( 11 , 12 ). (spandidos-publications.com)
  • C. pneumoniae induces the secretion of different kinds of cytokines and cell signalling molecules and the expression of adhesion molecules in all of these cell types. (helsinki.fi)
  • CTXA also inhibited expression and activity of TACE induced by PMA in endothelial cells. (sagepub.com)
  • In recent years, the MAPK family has been found to be an important signal-regulating enzyme between cell surface receptors and determinants of gene expression ( 8 ). (spandidos-publications.com)
  • Recently, controversy has developed over the definition of true endothelial progenitors. (wikipedia.org)
  • Moreover, MCT4 overexpression enhances cell migration and invasiveness via reorganization of the actin cytoskeleton. (hindawi.com)
  • The formation of new vessels in the hypoxic area restores blood flow, provides oxygen and nutriments to the surviving cells, and promotes the migration and engraftment of new cells. (elifesciences.org)
  • Infection of kidney cells with hantavirus species PUUV and HTNV causes a significant reduction of migration capacity. (biomedcentral.com)
  • The direct impairment of migration capacity of renal cells by hantaviral N proteins may contribute substantially to proteinuria observed in the clinical picture of hantavirus infection. (biomedcentral.com)
  • Ablation of the endothelial progenitor cells in the bone marrow lead to a significant decrease in tumour growth and vasculature development. (wikipedia.org)
  • CD133 + stem cells were purified from patient-derived sternal bone marrow using the recently developed automatic CliniMACS Prodigy® BM-133 System (Prodigy). (biomedcentral.com)
  • the CFU-Hill population is an early outgrowth, formed by plating peripheral blood mononuclear cells on fibronectin-coated dishes, allowing adhesion and depleting non-adherent cells, and isolating discrete colonies. (wikipedia.org)
  • Cell adhesion and individual cell motility play a pivotal role in the establishment and maintenance of cell-to-cell contacts, polarity, and renal filtration. (biomedcentral.com)
  • A large body of evidence now implicates increased leukocyte-endothelial cell adhesion as a key early event in the development of diabetic retinopathy. (diabetesjournals.org)
  • Furthermore, we noted much higher correlation, as compared with control subjects, between increased values of core 2 GlcNAc-T activity and cell adhesion properties. (diabetesjournals.org)
  • A nitric oxide (NO) detection kit was used to determine the concentration of NO. Western blotting analysis was performed to determine the levels of intercellular cell adhesion molecule (ICAM)‑1, vascular cell adhesion molecule (VCAM)‑1, endothelin‑1, caspase‑3, Bax, Bcl‑2, Toll‑like receptor 4 (TLR4), p65 and p‑p65. (spandidos-publications.com)
  • Homotypic interaction of CD31 is important in adhesion, cell-cell and cell-matrix interaction, and signal transduction. (thermofisher.com)
  • Hypoxia inducible factor-1 (HIF-1) is an oxygen-sensitive transcriptional regulator that regulates more than 70 downstream target genes, including vascular endothelial growth factor, erythropoietin, glucose transporter and so on ( 5 ). (spandidos-publications.com)
  • Exosomes microvesicles of endocytic source released by normal and tumor cells play an important role in cell-to-cell communication. (exposed-skin-care.net)
  • ECFCs are now known to be tissue-resident progenitor cells in adults that maintain some vasculogenic ability. (wikipedia.org)
  • Chicago - Genetically manipulated growth factors could hold the key to producing engineered tissue with vascular systems ready-made for transplantation. (dermatologytimes.com)
  • After one hour, no visible tissue pieces should be present and the solution will appear as a cloudy cell suspension. (jove.com)
  • Nutritional availability during fasting and refeeding affects the temporal redistribution of lymphoid and myeloid immune cells among the circulating and tissue-resident pools. (bvsalud.org)
  • Epidermal growth factor has been described in nearly 100% of meningiomas in tissue culture. (medscape.com)
  • Additionally, characterization of the tumor microenvironment such as interactions with immune cells remain largely unknown. (springer.com)
  • Previous studies have juxtaposed and analyzed immune cell compartments between different CCA categorizations such as short or long-term overall survival (OS) [ 16 ]. (springer.com)
  • Too strong cytokine and immune response is detrimental to cells and to whole system. (helsinki.fi)
  • The pellet is the stromal vascular fraction containing endothelial cells, immune cells, blood cells and progenitor cells. (jove.com)
  • Despite being exposed to periodic alterations in blood insulin levels upon fasting and feeding, studies exploring the physiological effects of these hormonal changes on quiescent immune cell function and trafficking are scanty. (bvsalud.org)
  • My research in cell physiology focuses on mitochondrial biology in the context of health, aging, and disease. (brocku.ca)
  • To learn about basic mitochondrial and cellular biology, understand mechanisms of disease, and develop drugs, cell culture is indispensable. (brocku.ca)
  • Cell Biology and Toxicology. (brocku.ca)
  • Vascular endothelial growth factor also seems to play a role in the biology of meningiomas. (medscape.com)
  • Discussion: In conclusion, our data indicate that bronchial and alveolar epithelial cells respond differently to hypoxia and profibrotic stimuli. (lu.se)
  • Experimental conditions have been defined that allow bovine corneal endothelial (BCE) cells to grow in the complete absence of serum. (nih.gov)
  • These cells are phenotypically similar to endothelial cells and have been shown to create vessel-like structures in vitro and in vivo. (wikipedia.org)
  • qRT-PCR confirmed that both IGF-1 and IGF-2 mRNA were increased in response to X-rays, and ELISA showed increased secretion of IGF-1 protein into the medium of irradiated HPAEC. (lonza.com)
  • Cylindrical vessel scaffolds were 3D printed using polylactic acid (PLA) material and the vessel lumen were inoculated with a co-culture of HUVEC and ADSC in a custom-built bioreactor system using cell growth medium ((DMEM, Corning®) and (ECM, Cell Applications Inc)). The vessels were installed in a sterile experimental flow loop facility capable of providing physiological flow forcing conditions. (aps.org)
  • Vero E6 cells were maintained in DMEM (Capricorn) supplemented with 10% FCS. (biomedcentral.com)
  • The Endothelial Growth Supplement is added to DMEM/F12 medium at a final concentration of 40ug supplement / mL of the medium. (gentaur.nl)
  • Hypoxia of CEC was induced by exposing the cells to cobalt chloride (Sigma-Aldrich, St. Louis MO) in serum media, as described previously [ 12 , 13 ], and the induction was confirmed by cytotoxicity and cell cycle analysis. (molvis.org)
  • That results in a growth factor that is vastly more potent as a mitogen than FGF or any other known mitogen. (dermatologytimes.com)
  • As far as LEV is concerned, in 2010 Bobustuc hypothesized that AED may modulate O-6 methylguanine-DNA methyltransferase (MGMT), a DNA repair protein that has an important role in tumor cell resistance to alkylating agents, and LEV was reported as the most potent MGMT inhibitor among several AED [ 10 ]. (biomedcentral.com)
  • Under physiological circumstances, the resistance of the endothelial cell lining to interactions with platelets and coagulation factors prevents thrombosis. (medscape.com)
  • Immunofluorescence Cells were prepared for fluorescence microscopy by permeabilization for 3 min with 0.1% Triton-X100 blocked with 5% BSA and. (exposed-skin-care.net)
  • Using a subviral particle vaccine candidate, we show here that one protein complex of HCMV, termed the pentameric complex (PC), enhances the neutralizing antibody response against viral infection of different cell types. (asm.org)
  • Here, we report that oral glucose load in mice and healthy men enhances the adherence of circulating peripheral blood mononuclear cells (PBMCs) and lymphocytes to fibronectin. (bvsalud.org)
  • Kim and Morshead, 2003), and the fibroblast that SSEA4 associated with CD133 can be used for growth factor (FGF) receptor 4 (Cai et al. (lu.se)
  • To facilitate this we developed the Mitochondrial Network Analysis (MiNA) toolset as a simple set of macros making use of existing ImageJ plug-ins that allows a semi-automated analysis of mitochondrial networks in cultured mammalian cells. (brocku.ca)
  • The family members are a group of evolutionarily conserved enzymes in mammalian cells, including four subclasses: ERK1/2, JNK, p38 and ERK5 ( 7 ). (spandidos-publications.com)
  • Induction of hypoxia was confirmed by flow cytometric analysis and the levels of SIRT1 were noted in a hypoxic condition as well in the cells after blocking SIRT1 activity using sirtinol. (molvis.org)
  • Hypoxic induction was confirmed using flow cytometric analysis, which showed cell cycle arrest starting at a 200 µM concentration of cobalt chloride. (molvis.org)
  • For example, in the retina uncontrolled and irregular blood vessel growth in diseases such as age-related macular degeneration and diabetic retinopathy can result in a catastrophic loss of vision. (ucl.ac.uk)
  • Dysregulation of this barrier function of endothelial cells is a hallmark feature of multiple diseases and conditions such as sepsis, cancer metastasis, and edema. (bvsalud.org)
  • The aim of this study was to investigate the effect of anti-chlamydial compounds on the release of cytokines and cell signaling molecule, nitric oxide, induced by C. pneumoniae -infection in different cell types. (helsinki.fi)
  • Nitric oxide measurements were also determined from the medium using Griess' reagent. (helsinki.fi)
  • Rapid medium nutrient depletion to below the physiological range by cancer cells cultured in Plasmax. (brocku.ca)
  • Our findings uncover the physiological relevance of post-prandial insulin spikes in regulating the adherence and trafficking of circulating quiescent T-cells through fibronectin-integrin interaction. (bvsalud.org)
  • It also has been shown that cell surface glypicans are low-affinity endostatin receptors (Karumanchi et al. (justia.com)