Endothelial cell cell line. Medical search. Frequent questions

Low PDCD4 level was associated with reduced proliferation but not apoptosis or phosphorylation of endothelial nitric oxide synthase caused by pulsatile shear stress to help maintain the homeostasis of endothelial cells. (plos.org)
Ample evidence shows that pro-atherosclerotic disturbed flow induces sustained activation of atherogenic genes in endothelial cells to promote their oxidation, inflammation, cell cycle progression and proliferation, whereas pulsatile shear stress tends to maintain endothelial cells in a quiescent and less proliferative state with a low level of oxidation and inflammation [16] , [17] . (plos.org)
VEGF, produced in large amounts by cancer cells during tumor growth, interacts with its receptors VEGFR1 and VEGFR2 thereby causing endothelial cell survival, proliferation, and sprouting [7] . (plos.org)
In addition, at 48H, BPs linked to inflammatory phenotypes were activated (confirming KEGG results), while BPs linked to extracellular matrix (ECM) structural organisation, endothelial cell proliferation, and collagen metabolism were repressed. (nature.com)
It is well established that insulin-like growth factor (IGF) signaling is important for cell responses such as mitogenesis, cell growth, proliferation, angiogenesis, and differentiation. (wikipedia.org)
In addition to providing biomechanical support to the islets, ECM components are required for β-cell development, proliferation, survival, and proper insulin secretion. (nature.com)
We demonstrate that UCN-01, at concentrations lower than those necessary to inhibit cancer cell growth, inhibit proliferation of human endothelial cells in vitro. (karger.com)
We conclude that UCN-01, at clinically relevant concentrations, exerts an anti-neovascularization effect by blocking two important steps in vessel formation: (1) the response of cancer cells to hypoxia, and (2) endothelial cell proliferation. (karger.com)
Hyaline membranes appear along with a proliferation of type 2 alveolar lining cells. (medscape.com)
1994. Mesothelial cell proliferation: A nonspecific response to lung injury associated with fibrosis. (cdc.gov)
Kaposi's sarcoma is a neoplasm of vascular endothelium that is characterized by proliferation of spindle cells, neoangiogenesis, inflammation and edema 17 . (bvsalud.org)

In vitro , the sprouting and migratory properties of primary endothelial cell cultures were reduced by inhibiting EZH2 through up-regulation of miR-101, siRNA-mediated knockdown of EZH2, or treatment with 3-Deazaneplanocin-A (DZNep), a small molecule inhibitor of EZH2 methyltransferase activity. (plos.org)
In vitro studies revealed similar cell permissiveness and replication of CSUR381 (compared with 2 other NiV isolates) in both bat and human cell lines. (cdc.gov)
To study the migration of microglia across the BBB, we developed an in vitro co-culture system of mouse brain endothelial cells (MBECs) and Ra2 microglia using Transwell inserts. (portlandpress.com)
In vitro studies show that the virus can infect various cell types such as endothelial cells and B-lymphocytes 15 . (bvsalud.org)
We have established a method allowing to study the cell-specific secretome of pericytes in vitro-using Turbo-ID, a mutant non-toxic biotin ligase (6). (lu.se)
In vitro cell culture systems are instrumental in the development of therapeutic targets. (cdc.gov)
A robust in vitro cell culture system supporting HBV and HDV infections (HBV/HDV) is a critical prerequisite to studying HBV/HDV pathogenesis, the complete life cycle of HBV/HDV infections, and consequently identifying new therapeutics. (cdc.gov)
In vitro cell culture models have evolved with significant improvements over several decades. (cdc.gov)

This study aims to clarify the time-dependent molecular pathways by which TMAO mediates endothelial dysfunction through transcriptomics and metabolomics analyses in human microvascular endothelial cells (HMEC-1). (nature.com)
SNAI2, an angiogenesis-related factor, was highly expressed under the high glucose condition and also led to viability and migration of human brain microvascular endothelial cells (HBMECs) [ 13 ]. (hindawi.com)

This reflects the fact that endothelial hyperpolarization promotes calcium influx, activating the endothelial isoform of nitric oxide synthase, while also down-regulating the activity of the membrane-bound NADPH oxidase, chief endothelial source of superoxide. (nih.gov)
The expression of caspase‑3, Bax, Bcl‑2, phosphorylated (p)‑p38MAPK/total (t‑)p38MAPK and p‑endothelial e nitric oxide synthase (eNOS)/t‑eNOS proteins were determined by western blotting. (spandidos-publications.com)
Researchers found all nine flavors were dangerous to cells in the laboratory at the highest levels tested and all the flavorings impaired nitric oxide production in endothelial cells in culture (outside of the body). (sciencedaily.com)
Nonsmokers' cells that were treated with menthol or a clove flavoring also had impaired nitric oxide production, suggesting those flavorings cause damage like that found in active smokers. (sciencedaily.com)
Menthol applied to the cells increased IL-6 at high concentrations and reduced nitric oxide even at low doses. (sciencedaily.com)

Human brain vascular pericytes treated with either scrambled or RGS5 targeting siRNA, will be co-cultured in a spheroid model of the BBB together with human brain endothelial cells and human astrocytes. (lu.se)
Results of their new study suggest that these malformations are a result of KRAS -induced activation of the mitogen-activated protein kinase-extracellular signal-regulated kinase (MAPK-ERK) signaling pathway in brain endothelial cells. (medscape.com)

The team also exposed commercially-available human aortic endothelial cells to the flavorings. (sciencedaily.com)

Endothelial dysfunction is a critical initiating factor contributing to cardiovascular diseases, involving the gut microbiome-derived metabolite trimethylamine N-oxide (TMAO). (nature.com)
The discrepancies between studies on TMAO-induced endothelial dysfunction may be attributed to different cell types and animal models used, including different metabolic backgrounds, as well as to distinct TMAO treatment concentrations and durations. (nature.com)
Endothelial dysfunction during diabetes has been previously reported to be at least in part attributed to increased oxidized low‑density lipoprotein (oxLDL) levels mediated by high glucose (HG) levels. (spandidos-publications.com)
These results suggest that rosmarinic acid can exert a protective effect against oxLDL‑mediated endothelial dysfunction under HG conditions by reducing the interaction between monocytes and ECs in addition to preventing monocyte diapedesis. (spandidos-publications.com)
A number of studies have previously suggested that vascular endothelial dysfunction caused by increased junctional permeability is a key event in the development and progression of atherosclerosis and atherosclerotic cardiovascular diseases ( 3 , 4 ). (spandidos-publications.com)
Chronic metabolic disorders, such as hyperglycemia and dyslipidemia, promote endothelial dysfunction and eventually leakage, resulting in increased monocyte diapedesis ( 5 - 7 ). (spandidos-publications.com)
Endothelial dysfunction during diabetes is closely associated with increased oxidized low-density lipoprotein (oxLDL) levels mediated by high glucose (HG) conditions ( 8 ). (spandidos-publications.com)
That's because ED is associated with endothelial dysfunction that occurs in cardiovascular problems. (cnn.com)
Endothelial dysfunction is a chemical breakdown of the thin layer of cells that line the blood vessels. (cnn.com)
Officers with higher post-traumatic stress disorder (PTSD) symptoms had a nearly two-fold reduction in brachial artery flow-mediated dilation, indicating greater impairment of endothelial function (physiologic dysfunction of the normal biochemical processes carried out by the cells which line the inner surface of blood vessels) than officers with fewer PTSD symptoms. (cdc.gov)

We report that, in addition to direct inhibitory effects on endothelial cell growth, UCN-01 abrogates hypoxia-mediated transactivation of HIF-1-responsive promoters in a prostate cancer cell line. (karger.com)

ESCs will eventually produce endothelial cells (ECs), which create the thin-walled endothelium that lines the inner surface of blood vessels and lymphatic vessels. (wikipedia.org)
Role of insulin-like growth factors in endothelium differentiation ECs derived from stem cells are the beginning of vasculogenesis. (wikipedia.org)
Important signaling factors for vasculogenesis are TGF-β, BMP4, and VEGF, all of which promote pluripotent stem cells to differentiate into mesoderm, endothelial progenitor cells, and then into mature endothelium. (wikipedia.org)
Not only is VEGF critical for mesoderm cells to become an EC, but also for EPCs to differentiate into mature endothelium. (wikipedia.org)
Endothelial inflammation increases the adhesiveness of monocytes to the endothelium in addition to increasing vascular permeability, promoting diabetic atherogenesis. (spandidos-publications.com)
The endothelium is the thin layer of cells that line the interior surface of blood vessels, forming an interface between circulating blood in the lumen and the rest of the vessel wall. (phys.org)
Other genes associated with essential hypertension are important for the normal function of the lining of blood vessels (the vascular endothelium ). (medlineplus.gov)
A patient with a healthy endothelium can endure the cell loss difference found in this study. (bmj.com)
The endothelium is the thin layer of simple squamous cells that lines the interior surface of blood vessels and lymphatic vessels, forming an interface between circulating blood or lymph in the lumen and the rest of the vessel wall. (onteenstoday.com)
Endothelium is the inner lining of the blood vessel wall that is in direct contact with circulating blood. (mayo.edu)
Dr. Katusic's studies are focused on understanding mechanisms responsible for normal endothelial function and repair of injured endothelium. (mayo.edu)
The recognition of endothelium in brain blood vessels as a major therapeutic target significantly advanced efforts to develop pharmacological therapies, as well as gene- and cell-based therapies. (mayo.edu)
This injury appears to be a consequence of the host's immunological response to viral antigens that have penetrated the endothelium by means of the cells' own integrins. (medscape.com)
Kaposi sarcoma is a vascular neoplasm composed of endothelium-lined vascular spaces and spindle-shaped cells. (bvsalud.org)

Altogether, by identifying a pro-angiogenic VEGF/miR-101/EZH2 axis in endothelial cells we provide evidence for a functional link between growth factor-mediated signaling, post-transcriptional silencing, and histone-methylation in the angiogenesis process. (plos.org)
We previously showed that VEGF signaling in primary endothelial cell cultures caused overexpression of VEGFR2 in a positive feed-forward loop, which is at least partly regulated by loss of miRNA-mediated control of VEGF receptor degradation. (plos.org)
Moreover, we found that glioblastoma cells, notorious for their VEGF production, elicited a similar response when co-cultured with endothelial cells [11] . (plos.org)
miR-10 Regulates the Angiogenic Behavior of Zebrafish and Human Endothelial Cells by Promoting VEGF Signaling. (ca.gov)
We found that miR-10 functions, in part, by directly regulating the level of fms-related tyrosine kinase 1 (FLT1), a cell-surface protein that sequesters VEGF, and its soluble splice variant sFLT1. (ca.gov)
The increase in FLT1/sFLT1 protein levels upon miR-10 knockdown in zebrafish and in HUVECs inhibited the angiogenic behavior of endothelial cells largely by antagonizing VEGF receptor-2 signaling. (ca.gov)
In previous studies, many signalling molecules have been identified that control angiogenesis, with the secreted protein vascular endothelial growth factor (VEGF) being considered as the master regulator. (ucl.ac.uk)

For example, an endothelial progenitor cell (EPC) is more specialized than an ESC, and an EC is more specialized than an EPC. (wikipedia.org)
The terms "progenitor cell" and "stem cell" are sometimes equated. (phys.org)

Using the endothelin-1 (ET-1)-stimulated elevation in cGMP in LLC-PK1 cells as a biological detector system for the conversion of big ET-1 (bET-1) to ET-1, we detected bET-1-converting activities in subcellular fractions from bovine aortic cultured endothelial cells (BAE) and rat brain. (aspetjournals.org)

Vascular endothelial cells, as a monolayer in direct contact with the flowing blood, bear the most of the wall shear stresses and have important homeostatic functions in response to stress [16] . (plos.org)
Furthermore, monocyte transmigration through the endothelial monolayer was significantly increased by oxLDL treatment under HG conditions. (spandidos-publications.com)
Subsequently, rosmarinic acid treatment significantly reversed oxLDL‑induced overexpression of adhesion molecules and monocyte‑EC adhesion, oxLDL‑induced endothelial junction hyperpermeability and monocyte transmigration through the endothelial monolayer under HG conditions, in a dose‑dependent manner. (spandidos-publications.com)
Endothelial cells (ECs) form a semi-permeable monolayer that separates the bloodstream from underlying tissues and regulate the infiltration of blood cells and proteins through the vessel wall ( 1 , 2 ). (spandidos-publications.com)
The addition of microglia or ATP led to the disruption of the MBEC monolayer and significantly decreased barrier function as measured by trans-endothelial electrical resistance (TEER) and electric cell-substrate impedance sensing (ECIS). (portlandpress.com)
In conclusion, we offer evidence that microglia migration across the brain endothelial cell monolayer is increased in the presence of ATP in a manner that involves MMP secretion. (portlandpress.com)
Endothelial toxicity was evaluated by quantifying pro-inflammatory mRNA expression, monolayer permeability, and wound closure. (cdc.gov)
Conditioned medium from CBox-treated macrophages induced significant elevation in endothelial cell pro-inflammatory mRNA expression, enhanced monolayer permeability and impairment of scratch healing in CXCR3 dependent manner. (cdc.gov)

Progenitor stem cells are committed to differentiating along a particular cell developmental pathway. (wikipedia.org)
Now, the team is going back a step further in the pathway leading to DESAT1 degradation, trying to understand how temperature changes are sensed inside the cell to lead to changes in fatty acid composition and consequent effects on the whole organism. (asbmb.org)
The death receptor pathway plays an important role in cell apoptosis ( 12 ). (spandidos-publications.com)
P-selectin, a granule membrane protein of platelets and endothelial cells, follows the regulated secretory pathway in AtT-20 cells. (rupress.org)
In contrast, the surface expression of P-selectin transfected into CHO cells, which do not have a regulated pathway of secretion, did not change with 8-Br-cAMP treatment. (rupress.org)
Oxidized carbon black nanoparticle s induce endothelial damage through C-X-C chemokine receptor 3-mediated pathway. (cdc.gov)

In a paper published online in the journal Biomaterials Science , a team from the laboratories of Rice bioengineer Jordan Miller and Baylor College of Medicine biophysicist Mary Dickinson showed how to use a combination of human endothelial cells and mesenchymal stem cells to initiate a process called tubulogenesis that is crucial to the formation of blood-transporting capillaries. (sciencedaily.com)
While tissue engineers have found dozens of ways to coax stems cells into forming specific kinds of cells and tissues, they still cannot grow tissues with vasculature -- capillaries and the larger blood vessels that can supply the tissues with life-giving blood. (sciencedaily.com)
Each of the trillions of living cells in the human body are constantly supplied with oxygen and nutrients by tiny blood vessels known as capillaries. (sciencedaily.com)
Measuring just a few thousandths of a millimeter in diameter, some capillaries are so narrow that individual blood cells must squeeze through them in single-file. (sciencedaily.com)
Capillaries are made entirely from networks of endothelial cells, the type of cell that lines the inner surface of every blood vessel in the human body. (sciencedaily.com)
In the process of tubulogenesis -- the first step to making capillaries -- endothelial cells undergo a series of changes. (sciencedaily.com)
First, they form small, empty chambers called vacuoles, and then they connect with neighboring cells, linking the vacuoles together to form endothelial-lined tubes that can eventually become capillaries. (sciencedaily.com)

Application of unidirectional pulsatile shear stress to human umbilical vein endothelial cells (HUVECs) decreased PDCD4 protein but not mRNA level. (plos.org)
Programmed cell death 4 (PDCD4) is an important tumor suppressor in the development of various human cancers [1] and inhibits translation rather than transcription. (plos.org)
Here, we report on a novel human endothelial cell-based recycling assay (HERA) that can be used for such pre-clinical screening. (nature.com)
Melero-Martin JM , Khan ZA , Picard A , In vivo vasculogenic potential of human blood-derived endothelial progenitor cells . (avma.org)
Endothelial cell damage in human and rabbit corneas stored in K-Sol without antioxidants. (bmj.com)
Knockdown of miR-10 led to premature truncation of intersegmental vessel growth (ISV) in the trunk of zebrafish larvae, while overexpression of miR-10 promoted angiogenic behavior in zebrafish and cultured human umbilical venous endothelial cells (HUVECs). (ca.gov)
However, the effects of NAC on LPS‑induced apoptosis of human umbilical vein endothelial cells (HUVECs) and the associated mechanisms are not well characterized. (spandidos-publications.com)
Thus, human endocrine cells are not in direct contact with the vascular BM components but with the invaginated peri-islet membrane. (nature.com)
Here we investigate the effect of material wrinkled topographies on the responses of human umbilical vein endothelial cells (HUVECs) and adhesion of monocytes to HUVECs. (rsc.org)
Scientists have considered using human stem cells to repair heart damage, but some research has suggested that such cells often don't survive when they are implanted in a person's body, according to the study. (livescience.com)
The work is an important step with fragile endothelial cells (ECs) made from "induced pluripotent stem cells," or iPSCs, a type of cell that can potentially be made from the cells of any human patient. (sciencedaily.com)
Our work has important therapeutic implications because we demonstrate utilization of human cells and the ability to live-monitor their tubulogenesis potential as they form primitive vessel networks," said study lead author Gisele Calderon, a graduate student in Miller's Physiologic Systems Engineering and Advanced Materials Laboratory. (sciencedaily.com)
However, acute xenograft rejection (AXR), driven by immune cell activation,continues to be a barrier in pig-to-human xenotransplantation. (iupui.edu)
NK cell tolerance occurs naturally in utero where human leukocyte antigen (HLA)-E and HLA-G are present. (iupui.edu)
Expressing HLA-E/G in xenografts may provide immune protection from human NK cell cytotoxicity. (iupui.edu)
Methods: This study aims to demonstrate the use of HLA class I molecules in inducing human NK cell tolerance. (iupui.edu)
Genetically modified pECs were co-cultured with human peripheral blood mononuclear cells (PBMCs) for E:T ratio of 10:1 for 2 hours. (iupui.edu)
Conclusion and Impacts: Co-expression of HLA-E and HLA-G in 5GKO pECs significantly reduced human NK cell degranulation, compared to 5GKO.HLA-E, 5GKO.HLA-G, and 5GKO pECs. (iupui.edu)
The scientists, working in a special hydrogel with properties they can change in a controlled manner, first grew a parent blood vessel from human blood vessel lining cells. (mpg.de)
Multi-omics biomarkers of response to the discovered drugs were identified using human breast cancer cell lines. (cdc.gov)
Recently, the development of the HepG2-NTCP sec+ cell line, expressing the sodium taurocholate co-transporting polypeptide receptor (NTCP) and self-assembling co-cultured primary human hepatocytes (SACC-PHHs) has opened new perspectives for a better understanding of HBV and HDV lifecycles and the development of specific antiviral drug targets against HBV/HDV infections. (cdc.gov)

Significantly, there are additional non-epithelial stromal cells (NES) that exist alongside TEC within thymic microenvironments, including multiple subsets of mesenchymal and endothelial cells. (frontiersin.org)
Broadly separated into mesenchymal and endothelial cells, NES have been implicated in thymus organogenesis, thymocyte development, tolerance induction and development/maintenance of epithelial stroma. (frontiersin.org)

If such channels are expressed by endothelial cells lining conduit arteries, glycine is likely to have anti-atherogenic activity. (nih.gov)
Why do arteries have smooth lining? (onteenstoday.com)
Arteries typically have a thicker tunica media than veins, containing more smooth muscle cells and elastic tissue. (onteenstoday.com)
Why is the lining of arteries thicker than the lining of veins? (onteenstoday.com)
Muscular arteries contain more smooth muscle cells in the tunica media layer than the elastic arteries. (onteenstoday.com)

1998. Activation of alveolar macrophages and peripheral red blood cells in rats exposed to fibers/particles. (cdc.gov)

Various signaling pathways control the behavior of endothelial cells-the cells that line the interior surface of blood vessels. (ca.gov)
Various signaling pathways control the behavior of endothelial cells, but their post-transcriptional dose-titration by miRNAs is poorly understood. (ca.gov)

Endothelial cells can be found throughout the whole vascular system and they also play a vital role in the movement of white blood cells ECs were first thought to arise from extraembryonic tissues because blood vessels were observed in the avian and mammalian embryos. (wikipedia.org)
Endothelial cell (EC) monolayers located in the inner lining of blood vessels serve as a semipermeable barrier between circulating blood and surrounding tissues. (rsc.org)
The prime function of the arterial smooth muscle cell (SMC) in adult individuals is to contract and relax, thereby regulating blood flow to target tissues. (onteenstoday.com)
Endothelial cells form a single cell layer that lines all blood vessels and regulates exchanges between the bloodstream and the surrounding tissues. (onteenstoday.com)
While many studies have demonstrated the importance of cTEC and mTEC during T-cell development, thymic stromal microenvironments also contain heterogenous non-epithelial stromal (NES) populations in a similar manner to that seen in peripheral lymphoid tissues. (frontiersin.org)
Tissue engineering, also known as regenerative medicine, is a field aimed at integrating advances in stem cell biology and materials science to grow transplantable replacement tissues and organs. (sciencedaily.com)
Ultimately, we'd like to 3-D print with living cells, a process known as 3-D bioprinting, to create fully vascularized tissues for therapeutic applications," said Miller, assistant professor of bioengineering. (sciencedaily.com)
In one channel, the scientists seed endothelial cells, which line blood vessels in natural tissues. (mpg.de)
When this has happened, the scientists deliver a growth factor cocktail of molecules that drive blood vessel growth in natural tissues through the second channel, whereupon the endothelial cells migrate into the hydrogel. (mpg.de)

In psoriasis, T H 1 cells, which secrete interferon gamma and tumor necrosis factor, predominate. (medscape.com)
In psoriasis and allergic contact dermatitis, T H 1 cells, which secrete interferon gamma and tumor necrosis factor, predominate. (medscape.com)

Abnormal endothelial cell migration is seen in various diseases, including rheumatoid arthritis, cancer and macular degeneration. (asbmb.org)
While adult lymphoid progenitor cells are produced by the bone marrow, their lineage commitment and development into mature T-cells is dependent on their migration into the thymus, where essential interactions with heterogeneous thymic stromal cells take place ( 1 ). (frontiersin.org)

Toxicity and functional assays were also performed on murine macrophage (RAW 264.7) and endothelial cell lines (C166) with and without pharmacological inhibitors. (cdc.gov)

Robert S. Fischer, Clare M. Waterman (2010) CIL:7428, Mus musculus, blood vessel endothelial cell, primary cell line cell. (cellimagelibrary.org)
We show that a microRNA called miR-10 regulates the behavior of endothelial cells during blood vessel formation by encouraging signals that promote blood vessel growth. (ca.gov)
Genetic experiments in zebrafish revealed that miR-10 functions, in part, by directly regulating the level of a protein called FLT1, which inhibits the behavior of endothelial cells that promotes new blood vessel growth. (ca.gov)
The apoptosis of endothelial cells is a key event that may impair the integrity of the vessel wall and lead to the formation of atherosclerotic plaques ( 9 ). (spandidos-publications.com)
Flavoring chemicals widely used in e-cigarettes and other tobacco products may be toxic to the cells that line and regulate blood vessel function. (sciencedaily.com)
Signals from endothelial cells organize the growth and development of connective tissue cells that form the surrounding layers of the blood-vessel wall. (onteenstoday.com)
In these diseased retinas the LRG1 protein is expressed by blood vessel endothelial cells, which line blood vessel walls. (ucl.ac.uk)
A team headed by biomedical engineer Dr Britta Trappmann from the Max Planck Institute for Molecular Biomedicine in Münster, Germany, has developed a cell culture system in which, for the first time, a functional blood vessel system is able to grow within a framework made of synthetic materials. (mpg.de)
A synthetic hydrogel in which endothelial cells (pink cell nuclei) form new blood vessels that grow from a parent blood vessel (upright on the left). (mpg.de)
The endothelial cells form contacts with each other and attach to their synthetic tissue environment in the channel, thus forming a parent blood vessel after about a day," explains Britta Trappmann. (mpg.de)
It's important to determine whether the mutation is specific to one type of vascular cell, for example, endothelial cells that line the lumen of vessels and regulate exchanges between tissue and blood and help prevent clotting, or vascular smooth muscle cells in the vessel wall that regulate the vessel diameter and adjust the blood flow like a faucet would. (medscape.com)

Methods and Results: We show that the highly conserved microRNA family encoding miR-10 regulates the behavior of endothelial cells during angiogenesis by positively titrating pro-angiogenic signaling. (ca.gov)
Since hypoxia activates hypoxia-inducible factor (HIF-1)-dependent transcription in cancer cells that, in a paracrine fashion, drive tumor angiogenesis, we investigated the effects of UCN-01 on HIF-1-responsive promoter constructs. (karger.com)

However, changes in cell signalling in response to hypoxia that are mediated by RGS5 stroke. (lu.se)

Apoptosis refers to the self-regulated and orderly death of cells controlled by genes in order to maintain the stability of internal environment ( 8 ). (spandidos-publications.com)
In contrast, culturing on Laminin-421 induced the expression of β-cell genes, including Ins1 , MafA , and Glut2 , and significantly improved glucose-stimulated insulin secretion. (nature.com)
5-gene-knock-out (5GKO) porcine endothelial cells (pECs) were transfected with HLA-E and HLA-G genes. (iupui.edu)
Our hypothesis was that maybe it's a somatic genetic disease" or due to a mutation in the genes of a single cell. (medscape.com)

Thus, we hypothesized that pericytes regulate β-cells through the production of BM components. (nature.com)
We further found that the pericytic laminin isoforms differentially regulate mouse β-cells. (nature.com)

They investigated the role played by the activation of so-called adhesion molecules in the cell membrane through which cells adhere to their surrounding environment. (mpg.de)

Here, we used single-cell transcriptomics analysis to compare embryonic and post-natal endothelial cells to investigate the potential of adult vasculature to be reprogrammed in hematopoietic stem cells. (biorxiv.org)

The results of CCK‑8 and flow cytometric analyses showed that pretreatment of HUVECs with NAC or p38MAPK inhibitor (SB203580) attenuated LPS‑induced decrease in cell viability and increase in cell apoptosis. (spandidos-publications.com)

Studies to date indicate that endothelial cells express glycine-activated chloride channels, which promote hyperpolarization of the endothelial plasma membrane. (nih.gov)
Studies have shown that periodontitis is associated with altered expression of inflammatory mediators in gingival fibroblasts and periodontal membrane cells ( 3 , 4 ). (spandidos-publications.com)
β-Cells depend on the islet basement membrane (BM). (nature.com)
The peri-islet membrane, which encapsulates the islets, separates the endocrine cells from the exocrine pancreas and serves as a barrier from immune cell infiltration to the islets. (nature.com)
The researchers first enriched the hydrogel tissue framework with varying amounts of peptides that activate a certain type of adhesion molecule found in the membrane of endothelial cells called integrins. (mpg.de)
Upon stimulation of these cells, P-selectin is translocated to the plasma membrane where it functions as a receptor for monocytes and neutrophils. (rupress.org)

Medina RJ , Barber CL , Sabatier F , Endothelial progenitors: a consensus statement on nomenclature . (avma.org)

In a previous study, it was reported that oxLDL treatment induced nucleotide‑binding domain and leucine‑rich repeat containing family, pyrin domain‑containing 3 inflammasome activation in endothelial cells (ECs) under HG conditions, in a manner that could be effectively reversed by rosmarinic acid. (spandidos-publications.com)
The effects of oxLDL‑mediated inflammasome activation on endothelial permeability under HG conditions, in addition to the effects of rosmarinic acid on these oxLDL‑mediated processes, also remain poorly understood. (spandidos-publications.com)

heart muscle cells and cells called endothelial cells, which line the interior surface of blood vessels. (livescience.com)

They also demonstrate the use of LLC-PK1 cells as a rapid assay that permits the sensitive detection and measurement of the formation of biologically active ET-1 from its precursor bET-1. (aspetjournals.org)
Cell viability was assessed using the Cell Counting Kit‑8 (CCK‑8) assay. (spandidos-publications.com)
The qRT-PCR was used to measure the expression levels of the miR-429 in the serums of the patients or cells, and CCK-8, wound healing assay, and tube formation assay were used to observe the effects of miR-429 on the phenotype of HBMECs. (hindawi.com)

Primary endothelial cells from transgenic mouse embedded in 3D collagen gels of ~ 100 Pa Young's modulus are exposed to 10 micromolar blebbistatin (myosin II ATPase inhibitor). (cellimagelibrary.org)

Although transcriptional similarities have been found between embryonic and adult endothelial cells, we found some key differences in term of transcription factors expression. (biorxiv.org)
There is a deficit of expression of Runx1, Tal1, Lyl1 and Cbfb in adult endothelial cells compared to their embryonic counterparts. (biorxiv.org)
During embryonic development, the programmed death of certain cells plays an essential role in shaping organs and allowing proper growth. (phys.org)

Vasculogenesis is new production of a vascular network from mesodermal progenitor cells. (wikipedia.org)
It is important to talk more about vasculogenesis because this is what makes ECs different from other types of cells that are found in the body. (wikipedia.org)
When vasculogenesis occurs the cells transform into different versions throughout the process to eventually become the earliest blood vessels. (wikipedia.org)

Natural killer (NK) cells, with inhibitory and activating receptors, play unique roles in AXR by promoting graft rejection or tolerance. (iupui.edu)

A series of caspases proteins have been shown to be activated by the death induced signal complex [a form of death ligand that binds to the corresponding death receptor on the cell surface ( 13 )], which in turn induces apoptosis. (spandidos-publications.com)
In order to mimic the natural environment of cells, many additional biomolecules and cells would have to be integrated into the model system in later steps - these may be signaling proteins, immune cells or cells to stabilize the blood vessels. (mpg.de)
AtT-20 cells are a mouse pituitary cell line that can store proteins in a regulated fashion. (rupress.org)

Endothelial cells line the entire circulatory system, from the heart to the smallest capillary. (phys.org)
The vascular BM surrounds the islet capillary network and directly contacts mouse endocrine cells. (nature.com)
The basic pathophysiological lesion of HPS, and indeed of all Hantavirus infections, is a generalized increase in capillary permeability that results from endothelial damage. (medscape.com)
We've confirmed that these cells have the capacity to form capillary-like structures, both in a natural material called fibrin and in a semisynthetic material called gelatin methacrylate, or GelMA," Calderon said. (sciencedaily.com)

Lung examination findings reveal an interstitial pneumonitis made up of edema fluid, mononuclear cells, and lymphocytes with polymorphonuclear leukocytes. (medscape.com)
Differences in the biological effects of crocidolite asbestos and two glass fibres on epithelial lung cells. (cdc.gov)

In summary, this study mechanistically demonstrated an increased biological potency of CBox particles and established the role of macrophage-released chemical mediators in endothelial damage. (cdc.gov)

Measuring a few millimeters to about 3 cm in diameter, the cysts of peliosis hepatis often lack a cell lining and are surrounded by hepatocytes. (msdmanuals.com)

Endothelial tissue is a specialized type of epithelium tissue (one of the four types of biological tissue in animals). (phys.org)
IMs are found in the space between the tissue stroma cells and contain, among other components, fibrillar collagens and fibronectin. (nature.com)
Which tissue is found in the lining of blood vessels? (onteenstoday.com)
Which tissue makes up the lining of blood vessels? (onteenstoday.com)
Patching up a failing heart is no easy task, but now, researchers are using a new combination of cells to make grafts of heart tissue . (livescience.com)
Using these two cell types, the researchers engineered strips of heart muscle tissue. (livescience.com)
Glaucoma leads to the loss of retinal ganglion cells and their axons but also to tissue remodelling which involves both the optic nerve head and the retina. (molvis.org)

The signals and receptors must function properly at all times so the cells will know what they are supposed to do (Fuchs & Chen 2013). (wikipedia.org)

ECs were treated with oxLDL under HG conditions in the presence or absence of ROS scavengers mitoTEMPO and NAC, p38 inhibitor SB203580, FOXO1 inhibitor AS1842856 or transfected with the TXNIP siRNA, before protein expression levels of intercellular adhesion molecule 1 (ICAM‑1), vascular cell adhesion molecule‑1 (VCAM‑1), phosphorylated vascular endothelial‑cadherin (VE‑cadhedrin), VE‑cadherin and zonula occludens‑1 (ZO‑1) were measured by western blotting. (spandidos-publications.com)
In addition, oxLDL‑stimulated ECs under HG conditions exhibited increased phosphorylated VE‑cadherin protein levels and decreased ZO‑1 protein expression levels compared with those in untreated ECs, suggesting increased endothelial permeability. (spandidos-publications.com)
UCN-01, a protein kinase C (PKC) inhibitor, induces growth arrest and apoptosis in cancer cells and was recently introduced in a phase I clinical trial. (karger.com)
It has shown that miR-429 could inhibit the development of colorectal cancer via targeting in large tumor suppressor kinase (LATS2), and decreased miR-429 could promote the expression of SRY-box transcription factor 2 (SOX2) and B-cell lymphoma 2 (BCL2) to attenuate the neuronal injury induced by accumulation of amyloid β -protein [ 12 ]. (hindawi.com)
P-selectin (PADGEM, GMP-140, CD62) is a transmembrane protein specific to alpha granules of platelets and Weibel-Palade bodies of endotheial cells. (rupress.org)
To investigate whether the mechanism of targeting of P-selectin to granules is specific for megakaryocytes and endothelial cells and/or dependent on von Willebrand factor, a soluble adhesive protein that is stored in the same granules, we have expressed the cDNA for P-selectin in AtT-20 cells. (rupress.org)
In conclusion, we provide evidence for the regulated secretion of a transmembrane protein (P-selectin) in a heterologous cell line, which indicates that P-selectin contains an independent sorting signal directing it to storage granules. (rupress.org)

The manipulation of endothelial cells to secrete protective factors could enhance cardioprotection. (mdpi.com)

Like stem cells, progenitor cells have a capacity to differentiate into a specific type of cell. (phys.org)
In contrast to stem cells, however, they are already far more specific: they are pushed to differentiate into their "target" cell. (phys.org)

TMAO treatment for either 24H or 48H induces reduced cell viability and enhanced oxidative stress. (nature.com)

Endothelial stem cells (ESCs) are one of three types of stem cells found in bone marrow. (wikipedia.org)
These parent stem cells, ESCs, give rise to progenitor cells, which are intermediate stem cells that lose potency. (wikipedia.org)
Stem cells have the unique ability make identical copies of themselves. (wikipedia.org)
For stem cells, this usually occurs through several stages, when a cell proliferates giving rise to daughter cells that are further specialized. (wikipedia.org)
Hematopoietic stem cells are crucial for the continuous production of blood cells during life. (biorxiv.org)
One option to get hematopoietic stem cells matching perfectly a patient is cellular reprogramming. (biorxiv.org)
Hematopoietic stem cells emerge from endothelial cells in blood vessels during embryogenesis through the endothelial to hematopoietic transition. (biorxiv.org)
Using a combination of gene expression profiling and gene regulatory network analysis, we found that endothelial cells from the pancreas, brain, kidney and liver appear to be the most suitable targets for cellular reprogramming into hematopoietic stem cells. (biorxiv.org)
Overall, our work provides an important resource for the rational design of a reprogramming strategy for the generation of hematopoietic stem cells. (biorxiv.org)
Cells in the blood such as immune cells, red blood cells and other vital cell types are constantly renewed from stem cells, the so-called hematopoietic stem and progenitor cells (HSPCs). (phys.org)
The most important difference between stem cells and progenitor cells is that stem cells can replicate indefinitely, whereas progenitor cells can only divide a limited number of times. (phys.org)

Therefore, the present study aimed to elucidate the mechanisms involved in oxLDL‑induced endothelial permeability and monocyte diapedesis under HG conditions, in addition to the potential effects of rosmarinic acid. (spandidos-publications.com)

In contrast, when the scientists blocked integrin function they observed that the cells only migrated individually. (mpg.de)

Anatomy49

Organisms12

Diseases15

Chemicals and Drugs98

Analytical, Diagnostic and Therapeutic Techniques and Equipment34

Phenomena and Processes49

Disciplines and Occupations1

Information Science3

Proliferation11

Vitro8

Microvascular endothelial cells2

Nitric5

Brain endothelial cells2

Aortic endothelial cells1

Dysfunction10

Effects on endothelial1

Endothelium14

VEGF7

Progenitor cell2

Bovine aortic1

Monolayer8

Pathway6

Capillaries7

Human21

Mesenchymal2

Arteries5

Macrophages1

Pathways2

Tissues9

Tumor necros2

Migration2

Murine1

Vessel11

Angiogenesis2

Hypoxia1

Genes4

Regulate2

Adhesion molecules1

Transcriptomics1

HUVECs1

Membrane6

Progenitors1

Activation2

Surface of blood vessels1

Assay3

Collagen1

Embryonic3

Vasculogenesis3

Inhibitory1

Proteins3

Capillary4

Lung2

Mediators1

Hepatocytes1

Tissue7

Signals1

Protein7

Secrete1

Differentiate2

Induces1

Stem cells11

Mechanisms involved1

Contrast1