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EndopeptidasesNeprilysinSubstrate SpecificitySerine EndopeptidasesThiorphanSensitivity and SpecificityPHEX Phosphate Regulating Neutral EndopeptidaseProtease InhibitorsMolecular Sequence DataCysteine EndopeptidasesAmino Acid SequenceMetalloendopeptidasesGlycopeptidesAntibody SpecificityKineticsHydrolysisNeurotensinLysostaphinSerine Proteinase InhibitorsAspartic Acid EndopeptidasesBase SequenceBinding SitesDipeptidesOligopeptidesSpecies SpecificityPeptidesAtrial Natriuretic FactorChromatography, High Pressure LiquidThiazepinesSequence Homology, Amino AcidPeptide FragmentsRecombinant ProteinsCloning, MolecularCathepsin BCarboxypeptidasesModels, MolecularAminopeptidasesElectrophoresis, Polyacrylamide GelExopeptidasesSubstance PHydrogen-Ion ConcentrationSequence AlignmentFlavobacteriumEnkephalin, LeucinePeptide HydrolasesBradykininBotulinum Toxins, Type AProtein BindingIndansHypophosphatemiaStructure-Activity RelationshipThermolysinMolecular WeightPeptidoglycanEscherichia coliProtein Structure, TertiaryCarboxypeptidases AATP-Dependent EndopeptidasesKidneyAmino AcidsBacterial ProteinsPropionatesOrgan SpecificityProtein PrecursorsSubtilisinsMutationCell LineProtein Processing, Post-TranslationalProtein ConformationChromatography, GelMutagenesis, Site-DirectedCattleFamilial Hypophosphatemic RicketsCatalysisCatalytic DomainAngiotensin-Converting Enzyme InhibitorsIsoflurophatePeptidyl-Dipeptidase ALeucyl AminopeptidaseRabbitsChromatography, Ion ExchangeDNA PrimersEnzyme StabilityPolymerase Chain ReactionKininsROC CurveCrystallography, X-RayAminocaproatesCathepsin CPredictive Value of TestsN-Acetylmuramoyl-L-alanine AmidaseDipeptidyl-Peptidases and Tripeptidyl-PeptidasesDNA, ComplementaryCell MembraneCathepsinsSwineTachykininsCastor BeanDNAReproducibility of Results
Substrate SpecificitySensitivity and SpecifiProteasesLysyl EndopeptidaseInhibitorsProteasePeptidesCleavageCleavesPeptidasesEnzymeNeprilysinProteinMMPsProteinsCatalyticDegradeActivityTypeSubstrateSerineCysteineProteinsPeptidaseZinc-dependentEnzymesPeptide bondsProlylCaspasesAntibody24.11MammalianCleavage siteSubunitAminoELISAsBroadCatalysisOligopeptidesFamilyAssayComplexFoundMouseGroupAnalysis
Substrate Specificity8
  • By combining 2 enzymes with gastric activity and complementary substrate specificity, it should be possible to increase the safe threshold of ingested gluten, thereby ameliorating the burden of a highly restricted diet for patients with celiac sprue. (nih.gov)
  • Using a positional scanning combinatorial library, it was found that the major substrate-specificity determinant is for Ala in the P2 position. (expasy.org)
  • Serine-type endopeptidase with a dual trypsin-like and chymotrypsin-like substrate specificity. (icr.ac.uk)
  • To date, more than 25 have been identified and are categorized into 6 groups according to their substrate specificity. (medscape.com)
  • This lipase has a broad substrate specificity, meaning it can hydrolyze a variety of fats and triglycerides into important free fatty acids required for healthy skin and hair. (charnyhealing.com)
  • purification, characterization and substrate specificity on oxidized insulin B-chain. (qmul.ac.uk)
  • Caspases are also a family of intracellular proteases but with a strict substrate specificity for cleavage at sites made up of an Asp residue [18]. (health-ground.com)
  • Our crystal captures a view of TYMV PRO in its polyprotein processing mode that reveals dual substrate specificity determinants. (datexis.com)
Sensitivity and Specifi1
  • However, the poor sensitivity and specificity of those markers and many other molecules are serious limitations for their routine use in both early (AJCC stage I and II) and advanced stages of melanoma (AJCC stage III and IV). (hindawi.com)
Proteases5
Lysyl Endopeptidase5
  • Lysyl endopeptidase ® is a serine protease that cleaves peptide bonds at the carboxy-terminus of lysine residues with high specificity. (fujifilm.com)
  • Taking advantage of its excellent specificity, Lysyl Endopeptidase ® is used for peptide fragmentation and peptide mapping for analysis of the primary structure of proteins. (fujifilm.com)
  • Lysyl Endopeptidase ® is especially designed for use with in-gel digestion and mass spectrometric analysis. (serva.de)
  • Lysyl Endopeptidase (LysC), originally isolated from the soil bacterium discovered by Masaki, et al. (serva.de)
  • An added feature of Lysyl Endopeptidase is its ability to retain complete activity after incubation in 4 M urea or in 0.1 % SDS solution for up to 6 hours at 30 °C. (serva.de)
Inhibitors3
Protease1
  • It has protease and esterase activities and wide specificity. (beisin88.com)
Peptides4
  • The product peptides derived from the endopeptidase activities of BoNTs are detected by matrix-assisted laser-desorption ionization time-of-flight mass spectrometry. (cdc.gov)
  • A series of reactions between synthesised peptides and ZIP were carried out in order to elucidate the size and specificity of the active site of the enzyme. (dcu.ie)
  • In terms of the size of the active site, the endopeptidase was found to have optimum activity when there were two more amino acids after the proline, with a fall-off in activity detected for the longer peptides. (dcu.ie)
  • They can be divided into Endopeptidases and terminal peptidases according to the way they degrade peptides. (beisin88.com)
Cleavage4
  • A serine endopeptidase that has specificity for cleavage at ARGININE . (nih.gov)
  • A CALCIUM-dependent endopeptidase that has specificity for cleavage at ARGININE that is near paired basic residues. (nih.gov)
  • Asparaginyl endopeptidases (AEPs) are versatile enzymes that in biological systems are involved in producing three different catalytic outcomes for proteins, namely (i) routine cleavage by bond hydrolysis, (ii) peptide maturation, including macrocyclisation by a cleavage-coupled intramolecular transpeptidation and (iii) circular permutation involving separate cleavage and transpeptidation reactions resulting in a major reshuffling of protein sequence. (silverchair.com)
  • First, its endopeptidase activity is required to proteolytically process 206K at the HEL/POL junctions to release the 66K polymerase, while a second cleavage at the PRO/HEL junction contributes to the regulation of viral RNA synthesis. (datexis.com)
Cleaves1
  • cleaves specifically the peptide bonds at the carboxy-terminal side of Lysine residues and S-aminoethylcysteine residues with a high degree of specificity, making it a valuable tool for protein sequence analysis and for proteome research. (serva.de)
Peptidases1
Enzyme4
  • We investigated a new combination enzyme product, consisting of a glutamine-specific endoprotease (EP-B2 from barley) and a prolyl endopeptidase (SC PEP from Sphingomonas capsulata), for its ability to digest gluten under gastric conditions. (nih.gov)
  • This gene encodes a protein precursor of the digestive enzyme pepsin, a member of the peptidase A1 family of endopeptidases. (nih.gov)
  • 2021). Article 14: The specificity of host-pathogen interactions with emphasis on the specific inhibitor enzyme synthesized in the invasion-defense process. (natural-foundation-science.org)
  • Moreover, the consequences of using selected natural substances, which increased the specific activity of NEP, were examined on the angiotensin-converting enzyme (ACE, EC 3.4.15.1) in order to observe the specificity of these substances for the NEP. (fu-berlin.de)
Neprilysin2
  • Die Neutrale Endopeptidase (Neprilysin, EC 3.4.24.11, NEP) ist eine Metallopeptidase, die ein großes Substratspektrum hat. (fu-berlin.de)
  • The neutral endopeptidase (neprilysin, EC 3.4.24.11, NEP), as a metallopeptidase with a large substrate spectrum, affects different pathological processes or diseases such as hypertonia, Alzheimer s disease, adiposity or pathologic changes of the prostate. (fu-berlin.de)
Protein3
  • Comparisons with Ssp2-Rap2a orthologues suggest that the specificity of these immunity proteins for neutralizing effectors is fold-dependent and that in cases where the fold is conserved sequence differences contribute to the specificity of effector-immunity protein interactions. (dundee.ac.uk)
  • What does procollagen C-endopeptidase enhancer protein 2 have to do with HDL-cholesteryl ester uptake? (nih.gov)
  • Structure-activity features of the protein were characterized, and functional studies predict bubaline pregnancy associated glycoprotein 2 as an inducible, extra-cellular, non-essential, N-glycosylated, aspartic pro-endopeptidase that is involved in down-regulation of complement pathway and immunity during pregnancy. (biomedcentral.com)
MMPs2
Proteins1
  • The structures of two immunity proteins, Rap1a and Rap2a, responsible for the neutralization of Ssp1 and Ssp2-like endopeptidases, respectively, revealed two distinct folds, with that of Rap1a not having previously been observed. (dundee.ac.uk)
Catalytic1
  • Functional assays confirm the identity of a catalytic cysteine in these endopeptidases and crystal structures provide information on the structure-activity relationships of Ssp1 and, by comparison, of related effectors. (dundee.ac.uk)
Degrade1
  • They degrade polypeptides and are distinguished by their substrate specificities. (eurogentec.com)
Activity1
  • The data suggested that our rLys-C have higher activity and specificity in long time reaction than the competitor's product. (fujifilm.com)
Type1
  • Here, the peptidoglycan endopeptidase specificity of two type VI secretion-system-associated effectors from Serratia marcescens is characterized. (dundee.ac.uk)
Substrate14
  • The substrate specificity of botulinum type B neurotoxin was studied using various analogues of VAMP-2 (60-94). (nih.gov)
  • Family of DPP-4-related proteases and their substrate specificities. (medscape.com)
  • Here, we designed specific substrate peptides and reconfigured gold nanoparticle-based lateral flow test strip (LFTS) to develop an endopeptidase-based lateral flow assay for the diagnosis of botulism. (springer.com)
  • Therefore, the zinc-dependent endopeptidase activity of BoNT/A was utilized to design a specific substrate peptide with a His-tag at the carboxyl terminal end and a biotin at the amino terminus (Fig. 1a ). (springer.com)
  • This is a widely expressed enzyme with broad substrate specificity. (anaspec.com)
  • Bacterial collagenases differ from vertebrate collagenases in that they exhibit broader substrate specificity (Peterkofsky 1982, Birkedal-Hansen 1987). (worthington-biochem.com)
  • Serine-type endopeptidase with a dual trypsin-like and chymotrypsin-like substrate specificity. (icr.ac.uk)
  • Investigations on oligopeptides have revealed that the substrate specificity strongly overlaps and differs only subtly between the individual members (Choe et al. (clinical-research-informatics.com)
  • Neprilysin (NEP) is a promiscuous zinc metalloprotease with broad substrate specificity and cleaves a remarkable diversity of substrates through endopeptidase action. (diamond.ac.uk)
  • To determine the basis for this substrate specificity, we have determined the crystal structure of SENP1 in isolation and in a transition-state complex with SUMO-2. (uea.ac.uk)
  • FAP shares substrate specificity with prolyl oligopeptidase (PREP), studied in (neuro)inflammation and neurodegeneration as well as cancer. (inra.fr)
  • 13. Marked differences between metalloproteases meprin A and B in substrate and peptide bond specificity. (nih.gov)
  • 18. Human and mouse homo-oligomeric meprin A metalloendopeptidase: substrate and inhibitor specificities. (nih.gov)
  • After being activated by lysyl endopeptidase, it cleaves a thioester substrate. (rndsystems.com)
Serine6
  • Inhibited by mercurial reagents and some inhibitors of serine endopeptidases. (cathdb.info)
  • Enterokinase also known as enteropeptidase is a membrane-bound enzyme serine protease that initiates the activation of pancreatic hydrolases by cleaving and activating trypsinogen and converting it into trypsin and has a specificity for the sequence (Asp)4-Lys-Ile . (anaspec.com)
  • A serine endopeptidase that has specificity for cleavage at ARGININE . (bvsalud.org)
  • A serine endopeptidase secreted by the pancreas as its zymogen, CHYMOTRYPSINOGEN and carried in the pancreatic juice to the duodenum where it is activated by TRYPSIN . (lookformedical.com)
  • A serine endopeptidase that is formed from TRYPSINOGEN in the pancreas . (lookformedical.com)
  • Proteolytic enzymes from the serine endopeptidase family found in normal blood and urine. (wakehealth.edu)
Cysteine3
Proteins4
  • Clostridial neurotoxins are bacterial endopeptidases that cleave the major SNARE proteins in peripheral motorneurons. (rupress.org)
  • Pepsin / ˈ p ɛ p s ɪ n / is an endopeptidase that breaks down proteins into smaller peptides . (wikipedia.org)
  • It is one of three principal endopeptidases (enzymes cutting proteins in the middle) in the human digestive system, the other two being chymotrypsin and trypsin . (wikipedia.org)
  • Hydrolysis of proteins with broad specificity similar to that of pepsin A, preferring hydrophobic residues at P1 and P1', but also cleaving Gly20!Glu in the B chain of insulin. (genome.jp)
Peptidase1
  • This peptidase hydrolyses oligopeptides beyond the N-terminal peptide bond which indicates that ApAAP exhibits endopeptidase activity. (kenyon.edu)
Zinc-dependent2
  • Neprilysin (NEP) is a neutral endopeptidase, a zinc-dependent metalloprotease that inactivates several peptide hormones. (anaspec.com)
  • It also inhibits the zinc-dependent endopeptidases, MMPs (Matrix metalloproteinases). (agscientific.com)
Enzymes1
  • Hydrolysates from exopeptidase enzymes such as Flavourzyme gave negative effect on HSF 1184 cell culture, while hydrolysates from endopeptidase enzymes were supplementary for HSF 1184 cell culture. (utm.my)
Peptide bonds1
  • Cleavage of peptide bonds with very broad specificity. (cathdb.info)
Prolyl1
Caspases1
  • Since the cleavage site within precursors matches the specificity of caspases , sequential activation of precursors by activated caspases can occur. (lookformedical.com)
Antibody1
  • Sandwich ELISA: Legumain/Asparaginyl Endopeptidase Antibody (M1) [H00005641-M02] - Detection limit for recombinant GST tagged LGMN is approximately 0.1ng/ml as a capture antibody. (novusbio.com)
24.112
Mammalian1
Cleavage site1
  • Proteinase K has no pronounced cleavage specificity and the preferential cleavage site is the peptide bond adjacent hydrophobic amino acids. (avivasysbio.com)
Subunit2
  • Cleavage of the VAMP-2 peptide was demonstrated by both botulinum type B neurotoxin (Km = 3.3 x 10(-4) M) and by its purified light subunit (Km = 3.5 x 10(-4) M). The endopeptidase displayed a pH optimum of 7.0-7.5 and was inhibited by greater than 0.2 M NaCl and greater than 0.05 M sodium phosphate. (nih.gov)
  • 4. Two alpha subunits and one beta subunit of meprin zinc-endopeptidases are differentially expressed in the zebrafish Danio rerio. (nih.gov)
Amino3
  • The light chain (~50 kD - amino acids 1-448) acts as a zinc (Zn 2+ ) endopeptidase similar to tetanus toxin with proteolytic activity located at the N-terminal end (see image below). (medscape.com)
  • The cleavage specificity of pepsin is broad, but some amino acids like tyrosine , phenylalanine and tryptophan increase the probability of cleavage. (wikipedia.org)
  • A carboxypeptidase that catalyzes the release of a C-terminal amino acid with a broad specificity. (lookformedical.com)
ELISAs1
  • The specificity from the ELISAs seem to be low in African populations (Truck Dyck et al. (exposed-skin-care.net)
Broad1
Catalysis1
  • In eukaryotic organisms there are up to seven different types of beta subunits, three of which may carry the N-terminal threonine residues that are the nucleophiles in catalysis, and show different specificities. (cathdb.info)
Oligopeptides1
  • In addition, all strains have 4-5 intact extra genes encoding OppA paralogs for binding of oligopeptides, possibly with different specificity. (ens-lyon.fr)
Family1
  • [ 3 ] The multiple members of the DPP-4 family mandate a careful assessment of the selectivity and specificity of any agent used to inhibit DPP-4 activity. (medscape.com)
Assay1
  • The as-prepared LFTS had excellent performance in the detection of botulinum neurotoxin using only 1 μL of simulated serum, and its sensitivity and specificity were comparable to that of mouse lethality assay. (springer.com)
Complex1
Found1
  • 1953), studies from the late 1950s to the mid 1980s found that several separable collagenases exist and these fractions' specificities and stabilities were partially characterized. (worthington-biochem.com)
Mouse1
Group1
Analysis1
  • In vitro analysis of specificity during nerve-muscle synaptogenesis. (nyu.edu)