• An E. Coli strain that carries an MBP fusion clone of Bovine Pancreatic DNase I. (neb.com)
  • DNase I (Deoxyribonuclease I ) is a dialyzed lyophilized powder from bovine pancrease that has been purified by chromatography Bovine pancreatic dexoy ribonuclease I (DNase I) is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding polynucleotides with a free hydroxyl group at the 3' position and phosphate group at the 5' position. (gendepot.com)
  • Aprotinin is a bovine pancreatic trypsin inhibitor (BPTI) inhibitor which inhibits trypsin and chymotrypsin with K i s of 0.06 pM and 9 nM, respectively. (medchemexpress.com)
  • The RNase Inhibitor is intended for molecular biology applications. (qiagen.com)
  • 20,000 U of RNAse Inhibitor (0.5 mL at 40,000 U/mL). (qiagen.com)
  • Non-competitive inhibitor of pancreatic-type ribonucleases e.g. (qiagen.com)
  • RNase Inhibitor is an acidic, 52 kDa protein that is a potent non-competitive inhibitor of pancreatic-type ribonucleases such as RNase A, RNase B and RNase C. The enzyme is provided as a fusion of the porcine RNase Inhibitor gene with a proprietary 22.5 kDa protein tag. (qiagen.com)
  • Instructions for using RNase Inhibitor are provided in the corresponding kit protocol in the resources below. (qiagen.com)
  • Dilutions of the enzyme were made in 1X RNase Inhibitor Reaction Buffer and added to 1000 μL reactions containing 1mM cytidine 2',3'-cyclic monophosphate, 1μg RNase A in a 1X reaction buffer containing 100mM Tris-Acetate, 1mM EDTA, pH 6.5. (qiagen.com)
  • Alternatively referred to as the RNase L inhibitor, this protein functions to block the activity of ribonuclease L. Activation of ribonuclease L leads to inhibition of protein synthesis in the 2-5A/RNase L system, the central pathway for viral interferon action. (nih.gov)
  • The 2'-5' oligoadenylate/RNase L/RNase L inhibitor pathway regulates both MyoD mRNA stability and muscle cell differentiation. (nih.gov)
  • cDNA cloning and expression analysis of the murine ribonuclease L inhibitor. (nih.gov)
  • XPF-ERCC1 is a highly conserved, heterodimeric, structure-specific endonuclease complex required for lesion excision in NER 26 , 27 that is thought to play an analogous role in the repair of DNA interstrand crosslinks (ICLs) that covalently link both DNA strands preventing transcription and replication 28 . (nature.com)
  • Ribonuclease pancreatic is an enzyme that in humans is encoded by the RNASE1 gene. (wikipedia.org)
  • Double-stranded endonuclease activity was determined in a 50 μL reaction containing 0.5 μg of pBR322 DNA and 10 μL of enzyme solution incubated for 4 hours at 37°C. (qiagen.com)
  • It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1 (IRE1). (cancerindex.org)
  • This forms a hairpin precursor of approximately 60-70 nucleotides, termed pre-microRNA, which is exported to the cytoplasm and modified by another enzyme, the RNAseII endonuclease, Dicer, to form a duplex of mature microRNA. (biomedcentral.com)
  • The encoded endonuclease cleaves internal phosphodiester RNA bonds on the 3'-side of pyrimidine bases. (wikipedia.org)
  • DNase I, (RNase-free) is an endonuclease that nonspecifically cleaves DNA to release di-, tri- and oligonucleotide products with 5´-phosphorylated and 3´-hydroxylated ends (1,2). (neb.com)
  • endonuclease (CuquEndo) produced in mammalian cells showed no sequence specificity for DNA substrate except that it only cleaves double-stranded DNA. (silverchair.com)
  • Homo sapiens pancreatic and duodenal homeobox 1 (PDX1), mRNA. (dbcls.jp)
  • Among these TFs is Pancreatic and duodenal homeobox 1 (PDX1), which recruits a unique subset of transcriptional coregulators to modulate its activity. (bvsalud.org)
  • This gene encodes a member of the pancreatic-type of secretory ribonucleases, a subset of the ribonuclease A super-family. (wikipedia.org)
  • Therefore, while GLUT2 levels are unchanged or even upregulated in several tissues such as the liver and the intestine during the hyperglycemic conditions observed in diabetes, the same gene undergoes a drastic dysregulation only in the pancreatic islets. (justia.com)
  • The presence of GTI, II and III are both sufficient and necessary to confer pancreatic-specific expression to a reporter gene in vitro or in vivo, using a transgenic mice approach (3,51). (justia.com)
  • The IB1 gene is highly expressed in the pancreatic islets and in the brain and to a much lesser extent in the heart and the kidney. (justia.com)
  • Contribution of disulfide bonds to the conformational stability and catalytic activity of ribonuclease A". European Journal of Biochemistry. (wikipedia.org)
  • Enhanced expression of RNase L as a novel intracellular signal generated by NMDA receptors in mouse cortical neurons. (nih.gov)
  • In contrast, mRNA levels are now shown not to change by both Northern and RNAse protection assays. (embl.de)
  • Enzymes recognizing helical parameters such as groove width and flexibility(Dnase-I) or helical distortions caused by abasic sites (exonuclease III,HAP1), and enzymes specific for such structures as flap DNA (5′nucleases of eukaryotes and phage and eubacterial DNA polymerases) and four-way junctions (T4 endonuclease VII, RuvC) have been reported (reviewed by Suck, 1997 ). (silverchair.com)
  • Expression of mRNAs of pancreatic and L type RNase inhibitors as a function of age in different tissues of SAMP8 and BDF1 mice. (nih.gov)
  • These observations are critical since several experimental models of diabetes have shown that GLUT2 expression is dramatically reduced specifically in the pancreatic .beta. (justia.com)
  • The GLUT2 facilitated glucose transporter isoform is a membrane protein present in the pancreatic .beta. (justia.com)
  • Apurinic/apyrimidinic endonuclease 1/redox effector factor 1 (APE1/Ref-1) is a multifunctional protein that is essential in activating oncogenic transcription factors. (oncotarget.com)
  • endonuclease was active in the presence of Mg 2+ ions at pH 7.0-8.0,but no endonuclease activity was detected in the presence of calcium ions. (silverchair.com)
  • Previous analysis of the salivary gland transcriptome of Culex quinquefasciatus showed the potential presence of an endonuclease with sequence similarities to shrimp, crab and two tsetse salivary proteins. (silverchair.com)
  • In pancreatic islets, GLUT2 allows a rapid equilibration of glucose between the extracellular space and the interior of the cells and it may play a crucial role in the glucose signaling mechanism leading to insulin secretion (43). (justia.com)
  • pancreatic cells to glucose remains debated. (justia.com)
  • Mrr from Escherichia coli K12 is a type IV restriction endonuclease whose role is to recognize and cleave foreign methylated DNA. (uci.edu)
  • Editing efficiency can be analyzed using ArciTect™ T7 Endonuclease I Kit ( Catalog #76021 ) or flow cytometry, if the experimental design permits. (stemcell.com)
  • Although endonucleases have been associated with a variety of cellular functions, their role in mosquito saliva is not clear. (silverchair.com)
  • This female-specific secreted endonuclease may assist blood meal intake by lowering the local viscosity created by the release of host DNA in the bite site and/or acting as an indirect anticoagulant factor by producing a defibrotide-like mixture of DNA haptamers. (silverchair.com)
  • This factor is abundantly expressed in the pancreatic islets and in the brain and has been named IB-1 for Islet-Brain 1. (justia.com)
  • Ribonuclease pancreatic is an enzyme that in humans is encoded by the RNASE1 gene. (wikipedia.org)
  • An enzyme that catalyzes the endonucleolytic cleavage of pancreatic ribonucleic acids to 3'-phosphomono- and oligonucleotides ending in cytidylic or uridylic acids with 2',3'-cyclic phosphate intermediates. (nih.gov)
  • RNase I catalyzes the hydrolysis of single-stranded RNA to nucleoside 3'-monophosphates via 2', 3' cyclic monophosphate intermediates. (mclab.com)
  • SAMHD1, a global restriction factor, and FEZ1, a brain-specific factor, have been shown to restrict viral replication by endonuclease/ATPase activity, and inhibiting nuclear import, respectively. (rochester.edu)
  • BbvCI restriction endonucleases. (bvsalud.org)
  • Mrr from Escherichia coli K12 is a type IV restriction endonuclease whose role is to recognize and cleave foreign methylated DNA. (uci.edu)
  • Escherichia coli ribonuclease I] catalyzes the degradation of single stranded polyribonucleotides to nucleoside 5'-monophos- phates (1-3). (nih.gov)
  • RNase H is an endoribonuclease that degrades the RNA strand of RNA/DNA hybrid molecules. (mclab.com)
  • Eosinophil-derived neurotoxin is a RIBONUCLEASE and may play a role as an endogenous antiviral agent. (nih.gov)