Endogenous mirna hepatocellular carcinoma cells micro rna that regulates. Medical search. Frequent questions

The human genome contains over 1000 miRNAs, each one targeting hundreds of different genes. (wikipedia.org)
It is estimated that half of all genes of the genome are targets of miRNA, spanning a large layer of regulation on a post-transcriptional level. (wikipedia.org)
These competitive endogenous RNAs (ceRNAs) act as molecular sponges for a microRNA through their miRNA binding sites (also referred to as miRNA response elements, MRE), thereby de-repressing all target genes of the respective miRNA family. (wikipedia.org)
EPCs from CB expressed abundant genes involved in cell cycle, hypoxia signalling and blood vessel development, correlating with the phenotypes that CB-EPCs proliferated more rapidly, migrated faster, and formed tubule structure more efficiently. (biomedcentral.com)
Stimulating the expression of angiogenic microRNAs or genes in EPCs of low activity (such as those from patients with cardiovascular diseases) might allow the development of novel therapeutic strategies. (biomedcentral.com)
Consequently, we hypothesized that some cancer-driver genes targeted by miR-31 are up-regulated in HCC as miR-31 was down-regulated in Anisole Methoxybenzene HCC. (nos-nop.org)
Next, to verify that miR-31 particularly binds to 3UTRs of also to interfere translation of these transcripts, mutant vectors harboring random mutation sequences of miR-31 biding sites from the 3UTR of and genes had been generated, and each vector was co-transfected with miR-31 into SNU-449 and SKHep-1 cells. (nos-nop.org)
Most of the existing research has focused on genes for prognosis prediction, whereas pathways based on mRNAs or miRNAs have never been used to predict survival. (aging-us.com)
Long non-coding RNA small nucleolar RNA host genes (SNHGs) play a critical role in the occurrence and development of tumors. (xiahepublishing.com)
We observed that EGFRvIII expression alters the expression of EV-regulating genes (vesiculome) and EV properties, including their protein composition. (cancer-genetics.org)
The multifunctional regulator nuclear factor erythroid 2-related factor (Nrf2) is considered not only as a cytoprotective factor regulating the expression of genes coding for anti-oxidant, anti-inflammatory and detoxifying proteins, but it is also a powerful modulator of species longevity. (springer.com)
The discovery of the antioxidant response element (ARE) have led to the conclusion that the battery of genes, including glutamate-cysteine ligase (GCL), thioredoxin reductase 1 (Txnrd1), NAD(P)H-quinone oxidoreductase 1 (NQO1) and heme oxygenase-1 (HMOX1) is regulated through Nrf2 binding to this consensus binding sequence [ 3 ]. (springer.com)
Mechanistically, lncRNAs have been characterized as sponges with microRNAs (miRNAs) or circular RNAs (circRNAs) and act as competing endogenous RNAs (ceRNAs) to reduce the regulation of their target genes [ 15 ]. (biomedcentral.com)
We previously identified differentially expressed microRNAs with liver-specific target genes in plasma from children with chronic hepatitis B. To further understand the biological role of these microRNAs in the pathogenesis of chronic hepatitis B, we have used the human liver cell line HepG2, with and without HBV replication, after transfection of hepatitis B virus expression vectors. (biomedcentral.com)
RT-qPCR is the preferred method for microRNA studies, and a careful normalisation strategy, verifying the optimal set of reference genes, is decisive for correctly evaluating microRNA expression levels. (biomedcentral.com)
The aim of this study was to provide valid reference genes for the human HCC-derived cell line HepG2. (biomedcentral.com)
A panel of 739 microRNAs was screened to identify the most stably expressed microRNAs, followed by a PubMed search identifying microRNAs previously used as reference genes. (biomedcentral.com)
We identified miR-24-3p, miR-151a-5p, and miR-425-5p as the most valid combination of reference genes for microRNA RT-qPCR studies in our hepatitis B virus replicating HepG2 cell model. (biomedcentral.com)
Results: In our study, 4 circRNAs, 3 miRNAs, and 6 genes that may be closely related to AML were detected. (istanbul.edu.tr)
(B) effect of miR-503 on the expression of endogenous genes. (biomedcentral.com)
From the list of genes that were targeted by the 73 diagnostic miRNAs, DGKE and WDR47 had significant associations with responses to both systemic therapies and radiotherapy in lung cancer. (cdc.gov)
Experimentally confirmed target genes were identified for the 73 diagnostic miRNAs, from which proliferation genes were selected from CRISPR-Cas9/RNA interference (RNAi) screening assays. (cdc.gov)
Generally, the regulation of target genes by miRNAs occurs through binding with the 3′-untranslated region (UTR). (spandidos-publications.com)
Dynamic Transcription of Long Non-Coding RNA Genes during CD4+ T Cell Development and Activation. (aksomics.com)
Molecular (DNA) Detection Current ways of detecting circulating parasites by demonstrating parasite DNA through amplification of ribosomal RNA (rRNA) genes by PCR assays represent the entire gold regular of malaria diagnostics. (tam-receptor.com)
MicroRNAs (miRNAs) are short (about 21 nucleotides long) strands of non-coding RNA that regulate the expression of multiple genes [ 1 ]. (biomedcentral.com)

This protein contains two N-terminal RNA-recognition motifs (RRMs), consistent with the observation that it binds directly to pre-mRNA. (nih.gov)
Luciferase reporter assay was performed to verify that miR-455 directly binds to the 3′-noncoding region (UTR) of RAB18 mRNA in Huh7 cells. (hindawi.com)
miR-455 inhibited cell viability and invasion by directly targeting the 3′-UTR of RAB18 mRNA of hepatocellular carcinoma. (hindawi.com)
MicroRNAs (miRNAs) are small noncoding endogenous RNAs containing 19 to 25 nucleotides that promote posttranscriptional control in regulating the expression of target gene by binding to the 3′-UTR sequences of its mRNA [ 5 ]. (hindawi.com)
Some models focus on mRNA 3' UTRs as targets, and others consider long non-coding RNA targets as well. (wikipedia.org)
The seed region, which comprises nucleotides 2-8 of the 5' portion of the miRNA, is particularly crucial for mRNA recognition and silencing. (wikipedia.org)
Recent studies have shown that the interaction of the miRNA seed region with mRNA is not unidirectional, but that the pool of mRNAs, transcribed pseudogenes, long noncoding RNAs (lncRNA), circular RNA (circRNA) compete for the same pool of miRNA thereby regulating miRNA activity. (wikipedia.org)
The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. (cancerindex.org)
The fact that and are up-regulated in HCC led us to hypothesize that normal and expressions are balanced by endogenous miR-31, which selectively regulates and mRNA translation in normal hepatic liver cells. (nos-nop.org)
Gene set variation analysis (GSVA) was used to establish and validate a miRNA set-based pathway prognostic signature for PC (miPPSPC) and a mRNA set-based pathway prognostic signature for PC (mPPSPC) in independent datasets. (aging-us.com)
Significant miRNA families were identified from mRNA-miRNA and lncRNA-miRNA interaction network analyses followed by stage specific mRNA-miRNA-lncRNA association network generation. (cancerindex.org)
0.05, log2FC≥0.5 (circRNA), and log2FC≥1 (miRNA and mRNA) from the raw data analyzed using the limma R package (v.3.46.0). (istanbul.edu.tr)
Identification of circRNA-lncRNA-miRNA-mRNA competitive endogenous rna network as novel prognostic markers for acute myeloid leukemia. (istanbul.edu.tr)
The seed region in miRNAs, approximately six nucleotides long, recognizes and binds to the complementary binding site of the target mRNA 3'untranslated region (3'UTR) to regulate its expression. (molcells.org)
The Long Intergenic Noncoding RNA UFC1, a Target of microRNA 34a, Interacts with the mRNA Stabilizing Protein HuR to Increase Levels of β-catenin in HCC Cells. (aksomics.com)
The Role of PinX1 in Growth Control of Breast Cancer Cells and Its Potential Molecular Mechanism by mRNA and lncRNA Expression Profiles Screening. (aksomics.com)
When we explored the mechanisms underlying these effects, we found that TUG1 acts as an endogenous competing RNA (ceRNA) that 'sponges' miR-145, thereby preventing the degradation of Sirt3 mRNA and increasing expression of Sirt3 and GDH proteins. (oncotarget.com)
Chen J, Wang S, Jia S, Ding G, Jiang G, Cao L. Integrated Analysis of Long Non-Coding RNA and mRNA Expression Profile in Pancreatic Cancer Derived Exosomes Treated Dendritic Cells by Microarray Analysis. (jcancer.org)
For example, exosomes and nanovesicles secreted by living cells serve as intercellular couriers of mRNA, microRNAs (miRNAs), long noncoding RNAs (lncRNAs), and proteins and have thus garnered considerable interest [ 3 ]. (jcancer.org)
Even though the role of circulating non coding RNAs as miRNA and lncRNA can be easily understood taking into consideration that can be secreted and persist in biofluid in remarkably stable forms, as "free" or associated to exosomes, less is known concerning the role of circulating mRNA, mainly taking into consideration its instability and the presence of RNase, nevertheless its presence is detected in blood. (scientificarchives.com)
In most cases, circRNAs are produced by back splicing of precursor messenger RNA (pre-mRNA), in which the downstream 5'-splicing donor is connected to the upstream 3'-splicing receptor by a 3'-5'-phosphodiester bond [ 11 ]. (biomedcentral.com)
Many of the phenotypic differences may also result from gene-environment interactions, referred to as epigenetics, a hereditable but reversible phenomenon that affects gene expression without modifying DNA sequence, such as alterations of DNA nucleotides (i.e., methylation), modifications of histones and regulation of transcription by altering mRNA stability through small RNA molecules such as microRNAs (miRNAs) [ 7 ] [ 8 ] . (encyclopedia.pub)

Emerging evidence indicates that Long non-coding RNAs (LncRNAs) and microRNAs (miRNAs) play crucial roles in tumor progression, including hepatocellular carcinoma (HCC). (biomedcentral.com)
Pseudogene, a subclass of long non-coding RNAs (lncRNAs), are considered as genomic loci that resemble real gene, but lost some functionality because they are lack of protein-coding ability because of disabling mutation, lack of transcription, or their inability to encode RNA [ 3 ]. (biomedcentral.com)
Bioinformatics algorithms including miRCODE ( http://www.mircode.org/ ) suggest that miRNAs can interact with lncRNAs. (biomedcentral.com)
A series of studies indicate that lncRNAs serve as competing endogenous RNAs (ceRNA) by sponging miRNAs, and modulate the targets of miRNAs [ 15 , 16 ]. (biomedcentral.com)
Previously, non-coding RNAs (ncRNAs), including micro-RNAs (miRNAs) and other long non-coding RNAs (lncRNAs), have been generally considered unable to encode proteins both in plants and animals [1-9]. (fortunepublish.com)
lncRNAs are well known to regulate a variety of biological process. (hindawi.com)
LncRNAs have been found to play key roles in a variety of cells in bone tissue. (biomedcentral.com)
Studies have shown that many lncRNAs are involved in the normal development or balance of the skeletal system, regulate the differentiation of osteoblasts, and participate in the occurrence of osteosarcoma [ 12 ]. (biomedcentral.com)
Mounting evidence supports that long noncoding RNAs (lncRNAs) have critical roles during cancer initiation and progression. (biomedcentral.com)
LncRNAs are endogenous cellular molecules with more than 200nt. (biomedcentral.com)
LncRNA-regulated infection and inflammation pathways associated with pregnancy loss: Genome wide differential expression of lncRNAs in early spontaneous abortion. (aksomics.com)
Long noncoding RNAs (lncRNAs) are important regulators in cancer progression. (oncotarget.com)
The expression profiles of long noncoding RNAs (lncRNAs) and mRNAs of PEX-treated dendritic cells are unknown. (jcancer.org)
Circulating RNAs are represented by a broad range of subtypes, such as microRNA (miRNAs), long non coding RNA (lncRNAs), messenger RNAs (mRNAs), transfer RNAs (tRNAs), snRNAs (small nuclear RNAs), snoRNA (small nucleolar RNAs), piwiRNAs (piRNA) and circRNAs (circular RNAs). (scientificarchives.com)
Circulating RNAs are represented by, such as for intercellular RNAs, a broad range of subtypes, mainly represented by microRNA (miRNAs), long non coding RNA (lncRNAs) and messanger RNAs (mRNAs). (scientificarchives.com)

Recently, increasing evidences elucidated that miRNAs was involved in the cancer pathogenesis, including cell proliferation, metastasis, and apoptosis [ 6 ]. (hindawi.com)
Also, in gastric cancer, miR-455 inhibited human cell proliferation and invasion and promoted cell apoptosis [ 12 ]. (hindawi.com)
In addition, miR-455 inhibited cell viability, while it induced cell apoptosis in colorectal cancer [ 13 ]. (hindawi.com)
EPCs from human umbilical cord and adult peripheral blood activate different mechanisms upon high-dose x-ray radiation treatment: CB-EPCs undergo p53 stabilization, Bax-dependent apoptosis and p21-dependent G 1 and G 2 /M cell cycle checkpoints, while PB-EPCs undergo only radiation-induced senescence [ 13 ], indicating unique gene expression patterns in EPCs of different sources. (biomedcentral.com)
Cell Counting Kit‑8 assay and flow cytometry were undertaken to measure proliferation and apoptosis. (spandidos-publications.com)
shlncRNA XIST attenuated cell proliferation, invasion and migration, while increasing the apoptosis of hepatic carcinoma cells. (spandidos-publications.com)
The miR‑320a mimic and PIK3CA inhibitor could recover the effect of oe‑lncRNA in terms of the proliferation, invasion, migration and apoptosis of hepatic carcinoma cells. (spandidos-publications.com)
Long non-coding (lnc)RNAs play important roles in the development, differentiation, proliferation and apoptosis of cells ( 6 , 7 ). (spandidos-publications.com)
Annexin V-PE/PI and PI staining were used to analyze cell apoptosis and cell cycle distribution by flow cytometry, respectively. (biomedcentral.com)
Functional experiments demonstrated that knockdown of PTTG3P inhibited cell proliferation, migration and invasion, and promoted cell apoptosis, acting as an oncogene. (biomedcentral.com)
Knockdown of KDM1B inhibits cell proliferation and induces apoptosis of pancreatic cancer cells. (cancerindex.org)
Circular RNA circ_0040823 inhibits the proliferation of acute myeloid leukemia cells and induces apoptosis by regulating miR-516b/PTEN. (istanbul.edu.tr)
Loss-of-function experiments support that PVT1 regulates cell apoptosis by influencing the DNA damage repair pathway after radiation, suggesting that targeting PVT1 may be a potential strategy for NPC therapy [ 11 ]. (biomedcentral.com)
Myocardial I/R injury may induce cell apoptosis and autophagy by activating oxidative stress and upregulating inflammatory mediators, ultimately resulting in irreversible fibrotic damage ( 3 ). (spandidos-publications.com)
MicroRNAs (miRNAs) are small endogenous RNAs that are associated with tumourigenesis, cell proliferation and apoptosis ( 4 , 5 ). (spandidos-publications.com)
This study was designed to determine the effects of the long non-coding RNA (lncRNA) plasmacytoma variant translocation 1 ( PVT1 ) on vascular smooth muscle cell (VSMC) apoptosis and extracellular matrix (ECM) disruption in a murine abdominal aortic aneurysm (AAA) model. (molcells.org)
Of note, the effects of Ang II in facilitating cell apoptosis, increasing matrix metalloproteinase (MMP)-2 and MMP-9, reducing tissue inhibitor of MMP (TIMP)-1, and promoting switching from the contractile to synthetic phenotype in cultured VSMCs were enhanced by overexpression of PVT1 but attenuated by knockdown of PVT1 . (molcells.org)
Furthermore, knockdown of PVT1 reversed Ang II-induced AAA-associated alterations in mice, as evidenced by attenuation of aortic diameter dilation, marked adventitial thickening, loss of elastin in the aorta, enhanced aortic cell apoptosis, elevated MMP-2 and MMP-9, reduced TIMP-1, and increased pro-inflammatory cytokines. (molcells.org)
SCARA5 overexpression significantly inhibited cell proliferation and induced apoptosis of CAL-27 and SCC-9 cells. (degruyter.com)
In conclusion, SCARA5 inhibited the proliferation and EMT and induced the apoptosis of OSCC cells through the inhibition of STAT3 and PI3K/AKT signaling pathways, thereby exerting a tumor suppressor effect. (degruyter.com)
MiR-155 also upregulates Mxd1/Mad1, a network of basic helix-loop-helix leucine zipper transcription factors which mediate cellular proliferation, differentiation, and apoptosis, through regulating BCL6. (biomedcentral.com)

miR-455 suppressed cell proliferation in vitro and in vivo, and it inhibited the abilities of cell invasion and EMT in HCC. (hindawi.com)
CCK-8 assay was performed to examine cell proliferation. (biomedcentral.com)
These are responsible for abnormal gene expression, resulting in increased cancer cell proliferation and escape from immune surveillance, which can speed up the process of development into HCC. (xiahepublishing.com)
Activation of PI3K/Akt signaling promotes glucose uptake, favors glycolysis and increases tumor cell proliferation. (drtaheriazam.com)
CCK-8, plate colony formation and EdU assays were performed to assess the cell proliferation. (biomedcentral.com)
In addition, SNHG4 or RRM2 knockdown significantly induced cell cycle arrest and cell senescence, and inhibited DNA damage repair and cell proliferation, and the effects can be partially reversed by let-7a knockdown or RRM2 reoverexpression. (biomedcentral.com)
12. Suer I, Kaya M. Is the AURKB gene involved in aml cell proliferation since it is targeted by miR-34a-5p and let-7b-5p? (istanbul.edu.tr)
In cell culture experiments, the influence of EPB41L4A-AS1 and UNC5B-AS1 on proliferation, migration, and invasion of the osteosarcoma cell line was examined by CCK-8 and Transwell assays. (biomedcentral.com)
A propulsive impact on cell proliferation, migration, and invasion by EPB41L4A-AS1 and UNC5B-AS1 was observed, but can be rescued by miR-1306-5p. (biomedcentral.com)
PVT1 promotes breast cancer cell proliferation and metastasis both in vitro and in vivo. (biomedcentral.com)
In addition, PVT1 binds to the UPF1 protein, thereby inducing epithelial-mesenchymal transition, proliferation and metastasis in breast cancer cells. (biomedcentral.com)
Knockdown of TUG1 inhibited the proliferation, motility, and invasiveness of cultured ICC cells, and decreased tumor burden in a xenograft mouse model. (oncotarget.com)

metastatic potential of HCC cells by selectively regulating epithelial-mesenchymal transition (EMT) regulatory proteins such as N-cadherin, E-cadherin, vimentin and fibronectin. (nos-nop.org)
HCC tissues derived from chemical-induced rat liver cancer models validated that miR-31 manifestation is significantly down-regulated, and that those cell cycle- and EMT-regulatory proteins are deregulated in rat liver cancer. (nos-nop.org)
Overall, we suggest that miR-31 functions like a tumor suppressor by selectively regulating cell cycle and EMT regulatory proteins in human being hepatocarcinogenesis providing a novel target FANCG for the molecular treatment of liver malignancies. (nos-nop.org)
Gene Ontology and pathway enrichment analyses and assessment of RNA binding proteins were performed by R software, circlncRNAnet and Encyclopedia of RNA Interactomes (ENCORI). (xiahepublishing.com)
ncRNAs are a large family of RNAs with microRNAs (miRNAs) being one kind of endogenous RNA which lack encoded proteins. (drtaheriazam.com)
Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. (cancer-genetics.org)
The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. (cancer-genetics.org)
This encoded protein is a cell surface glycoprotein that is known to complex with integrins and other transmembrane 4 superfamily proteins. (cancer-genetics.org)
Using mass spectrometry, quantitative proteomic analysis and Gene Ontology terms filters, we observed that EVs released by EGFRvIII-transformed cells were enriched for extracellular exosome and focal adhesion related proteins. (cancer-genetics.org)
Among them, we validated the association of pro-invasive proteins (CD44, BSG, CD151) with EVs of EGFRvIII expressing glioma cells, and downregulation of exosomal markers (CD81 and CD82) relative to EVs of EGFRvIII-negative cells. (cancer-genetics.org)
Nano-flow cytometry revealed that the EV output from individual glioma cell lines was highly heterogeneous, such that only a fraction of vesicles contained specific proteins (including EGFRvIII). (cancer-genetics.org)
The primary barriers for invading respiratory pathogens are the respiratory tract epithelial cells and antimicrobial proteins generated by these cells. (cdc.gov)
We also identified miRNAs that bind to receptor proteins, such as ACE2, ADAM17, and TMPRSS2, which are important for understanding the infection mechanism of SARS-CoV-2. (molcells.org)
First, RNA binding proteins (RBPs) bind the upstream and downstream introns. (biomedcentral.com)
These biomarkers are detectable in liquid biopsy, include circulating tumor cells (CTCs), proteins, cell free DNA (cfDNA) and RNA (cfRNA) that recently have acquired interest in cancer for their role in diagnosis and treatment response [ 1 , 3 ]. (scientificarchives.com)
They exert their biological functions by competitively binding to microRNAs (miRNAs) as miRNA sponges, promoting gene transcription and participating in the regulation of selective splicing, interacting with proteins and being translated into proteins. (biomedcentral.com)
They were nanoscale membrane vesicles that were generated by most types of cells, which can convey information in the tumor microenvironment by transferring cargos such as DNA, RNA, proteins and lipids, therefore they are critical to tumor progression [ 5 ]. (biomedcentral.com)
Single nucleotide polymorphisms (SNPs) in proteins regulating hepatocellular lipid handling, including Patatin-like Phospholipase Domain-containing 3 (PNPLA3), Transmembrane 6 Superfamily Member 2 (TM6SF2) and Membrane Bound O-acyltransferase Domain-containing 7 (MBOAT7), have been associated to NAFLD predisposition and progression towards NASH and fibrosis [ 6 ] . (encyclopedia.pub)

GSE94591, GSE116617, and GSE163386) were used to investigate dysregulated expressions of circular RNAs (circRNAs), and the GSE142699 and GSE142698 datasets were analyzed to detect dysregulated expressions of microRNAs (miRNAs) and mRNAs, respectively. (istanbul.edu.tr)
Circular RNAs (circRNAs) were first found in RNA (ribonucleic acid) viruses as a viroid as early as 1976 [ 1 ] and were later found to be an endogenous RNA splicing product in eukaryotes in 1979 as well [ 2 ]. (biomedcentral.com)
In the twenty-first century, with the development of RNA sequencing (RNA-seq) technologies and bioinformatics, the abundance and diversity of circRNAs was identified, and the dynamic expression patterns of circRNAs were revealed in various developmental stages and physiological conditions. (biomedcentral.com)
CircRNAs are divided into four categories: exonic circRNAs (ecircRNA), circular RNAs from introns, exon-intron circRNAs (EIciRNA) and intergenic circRNAs [ 21 ]. (biomedcentral.com)
Circular RNAs (circRNAs) are a new RNA type, deriving from back-splicing. (mdpi.com)
Circular RNAs (circRNAs) are a type of recently discovered noncoding RNA. (biomedcentral.com)
However, little attention has been given to exploring the value of circRNAs, as these RNAs were previously thought to be redundant products of faulty splicing [ 10 ]. (biomedcentral.com)
CircRNAs, a new type of endogenous noncoding RNA (ncRNA), are described as covalent closed-loop structure without 5' caps and 3' poly (A) tails. (biomedcentral.com)
In addition, several potential functions of circRNAs have been reported: (1) CircRNAs competitively bind to miRNAs as endogenous competitive RNAs (ceRNAs), known as miRNA sponges (Fig. 1 a). (biomedcentral.com)
METHODS Using high-throughput RNA sequencing (RNA-Seq), we conducted an in-depth bioinformatic analysis of noncoding RNAs (ncRNAs) in human cell-free saliva (CFS) from healthy individuals, with a focus on microRNAs (miRNAs), piwi-interacting RNAs (piRNAs), and circular RNAs (circRNAs). (scite.ai)

A long noncoding RNA critically regulates Bcr-Abl-mediated cellular transformation by acting as a competitive endogenous RNA. (aksomics.com)

An integrated approach for mining precise RNA-based cervical cancer staging biomarkers. (cancerindex.org)
Since international federation of gynecology and obstetrics (FIGO) staging is mainly based on clinical assessment, an integrated approach for mining RNA based biomarkers for understanding the molecular deregulation of signaling pathways and RNAs in cervical cancer was proposed in this study. (cancerindex.org)
MicroRNAs are regulatory molecules and suggested as non-invasive biomarkers for molecular diagnostics and prognostics. (biomedcentral.com)
MicroRNAs are suggested as non-invasive biomarkers for molecular diagnostics and prognostics, and some microRNAs show promising results as therapeutic targets in human trials [ 1 - 3 ]. (biomedcentral.com)
This review summarizes some information about categories, biogenesis, functions at the molecular level, properties of circular RNAs and the possibility of circular RNAs as biomarkers in cancers. (biomedcentral.com)
Long Non-Coding RNA Profiling in Laryngeal Squamous Cell Carcinoma and Its Clinical Significance: Potential Biomarkers for LSCC. (aksomics.com)
Recently, circulating RNAs (cfRNAs) are increasing their importance as biomarkers in liquid biopsy for cancer patients both for tumor characterization and development and for setting a monitoring personalized therapy. (scientificarchives.com)
BACKGROUND Extracellular RNAs (exRNAs) in human body fluids are emerging as effective biomarkers for detection of diseases. (scite.ai)

MicroRNAs are an abundant class of small, non-coding RNAs (~22nt long), which negatively regulate gene expression at the levels of messenger RNAs (mRNAs) stability and translation inhibition. (wikipedia.org)
microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. (cancerindex.org)
Moreover, these findings re-evaluated previous discoveries related to circulating mRNAs, that were found, for the first time in 1999 in plasma of nasopharyngeal carcinoma patients [ 13 ]. (scientificarchives.com)

Models for ceRNA regulation describe how changes in the expression of one or multiple miRNA targets alter the number of unbound miRNAs and lead to observable changes in miRNA activity - i.e., the abundance of other miRNA targets. (wikipedia.org)
editorializing] Hundreds of publications have described the influence of ceRNA regulation in normal and disease cells, but ceRNA regulation and its effects continue to be debated in scientific circles. (wikipedia.org)
Experimental evidence for such a ceRNA crosstalk has been initially shown for the tumor suppressor gene PTEN, which is regulated by the 3' untranslated region (3'UTR) of the pseudogene PTENP1 in a DICER-dependent manner. (wikipedia.org)
A new mechanism has recently been shown in which two closely spaced MREs (of the same or of different miRNA families) can cooperatively sequester miRNAs and thereby significantly boost a ceRNA effect. (wikipedia.org)
However, these studies were focused on one miRNA per context, and their leading researchers later identified physiologically-relevant ceRNA regulation of another miRNA. (wikipedia.org)
Both studies agree that determining the number of transcriptomic miRNA-binding sites is crucial for evaluating the potential for ceRNA regulation and that miRNA binding sites are generally higher than the number of miRNA molecules. (wikipedia.org)
2016) has revisited the discrepancies between the two studies and has shown that while miRNA levels define the extent of repression, they have little effect on the number of binding sites that need to be added to observe ceRNA-mediated regulation. (wikipedia.org)
Mechanistically, PTTG3P upregulated the expression of miR-383 targets Cyclin D1 (CCND1) and poly ADP-ribose polymerase 2 (PARP2) by sponging miR-383, acting as a competing endogenous RNA (ceRNA). (biomedcentral.com)
Bioinformatic analysis as well as experimental validation suggested that SNHG4 regulates RRM2 expression via a let-7 miRNA-mediated ceRNA network. (biomedcentral.com)

The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
The ncRNAs regulate various molecular pathways and mechanisms in cancer that can lead to induction/inhibition of carcinogenesis. (drtaheriazam.com)
A single microRNA can have multiple downstream targets and affect a number of different networks and pathways [ 4 , 5 ]. (biomedcentral.com)
(C) signaling pathways that regulate the cell cycle control in HCC involving miR-503. (biomedcentral.com)
In addition, Ingenuity Pathway Analysis (IPA) integrated with PCR array data showed that the JAK1/STAT3 pathway was significantly altered in cells overexpressing DEFB1, suggesting this to be one of the pathways by which defensin regulates IAV replication in HBEpCs. (cdc.gov)

Long non-coding RNA (lncRNA) is a class of RNA molecules with a length greater than 200 nt, a wide range of biological sources, and highly conserved secondary and tertiary structures [ 11 ]. (biomedcentral.com)
Dual-luciferase reporter assay and RNA pull-down experiments were used to examine the mechanisms interaction between molecules. (biomedcentral.com)

The expression of miR-455 was lower in HCC tissues and cell lines than in nontumor tissues and normal cell line, and downregulation of miR-455 was connected with worse outcome of HCC patients. (hindawi.com)
RAB18 was upregulated in HCC tissues and cell lines, and the expression of RAB18 was regulated by miR-455. (hindawi.com)
All the fresh tissues were snap-frozen in liquid nitrogen and stored at −80°C until RNA extraction. (hindawi.com)
lncRNA XIST was highly expressed in hepatic carcinoma tissues and cells. (spandidos-publications.com)
For instance, Deng et al ( 8 ) compared 66 pairs of hepatic carcinoma tissues and normal tissues and found that CCAT1 expression was increased in liver cancer tissues, and that the degree of upregulation was associated with tumor size, vascular invasion and serum alpha-fetoprotein level. (spandidos-publications.com)
In this study, we found that LIM-only protein 3 (LMO3) is hypomethylated and overexpressed in glioma cells and tissues. (oncotarget.com)
In this study, we found that PTTG3P was up-regulated in HCC tissues and cells. (biomedcentral.com)
Recent research has revealed that PVT1 is up regulated in nasopharyngeal carcinoma tissues and its overexpression predicts a poor prognosis for NPC patients. (biomedcentral.com)
Simple Summary: This study identified a set of 73 microRNAs (miRNAs) that can accurately detect lung cancer tumors from normal lung tissues. (cdc.gov)
Based on the consistent expression patterns associated with patient survival outcomes and in tumors vs. normal lung tissues, 10 miRNAs were considered to be putatively tumor suppressive and 4 miRNAs were deemed as oncogenic in lung cancer. (cdc.gov)
This study identified a set of 73 microRNAs (miRNAs) that classified lung cancer tumors from normal lung tissues with an overall accuracy of 96.3% in the training patient cohort (n = 109) and 91.7% in unsupervised classification and 92.3% in supervised classification in the validation set (n = 375). (cdc.gov)
Five miRNAs (hsa-miR-15b-5p, hsa-miR-195-5p, hsa-miR-221-3p, hsa-miR-140-3p, and hsa-miR-422a) showed differential expression patterns in lung tissues before and after infection. (molcells.org)
After Ang II infusion, mouse serum levels of pro-inflammatory cytokines were analysed, and aortic tissues were isolated for histological, RNA, and protein analysis. (molcells.org)
Total RNA was extracted from tissues of 78 patients with epithelial ovarian cancer, 17 normal ovarian epithelium tissues and two ovarian cancer cell lines. (scite.ai)

More specifically, they can act as microRNA sponges, protein sponges or scaffolds, and encode for peptides. (mdpi.com)

Oral squamous cell carcinoma (OSCC) is a common tumor in the world. (degruyter.com)

In molecular biology, competing endogenous RNAs (abbreviated ceRNAs) regulate other RNA transcripts by competing for shared microRNAs (miRNAs). (wikipedia.org)

Genome-wide analysis of long noncoding RNA in primary nasopharyngeal carcinoma by microarray. (aksomics.com)

The biogenesis, functions, and challenges of circular RNAs. (istanbul.edu.tr)
Role of circular RNA DLEU2 in human acute myeloid leukemia. (istanbul.edu.tr)
Circular RNA CREBBP suppresses hepatic fibrosis via targeting the hsa-miR-1291/LEFTY2 axis. (istanbul.edu.tr)
Global circular RNA expression profile of human gastric cancer and its clinical significance. (istanbul.edu.tr)
Circular RNAs, a novel class of endogenous noncoding RNAs, are characterized by their covalently closed loop structures without a 5′ cap or a 3′ Poly A tail. (biomedcentral.com)
Although the mechanisms of circular RNAs' generation and function are not fully clear, recent research has shown that circular RNAs may function as potential molecular markers for disease diagnosis and treatment and play an important role in the initiation and progression of human diseases, especially in tumours. (biomedcentral.com)
Unlike linear RNAs, circular RNAs have a special circular covalently bonded structure, which give them a higher tolerance to exonucleases. (biomedcentral.com)
As a result, an RNA double lariat and a circular RNA are produced. (biomedcentral.com)
Finally, some introns of the circular RNA are removed, producing an ecircRNA or EIcirRNA. (biomedcentral.com)
Thus, a circular RNA is produced. (biomedcentral.com)

However, they differ in two aspects: (1) the experimental approaches used to determine the number of effective transcriptomic miRNA-binding sites and (2) the impact miRNA concentrations have on the number of binding sites that must be added to detect target gene derepression. (wikipedia.org)
Another level of regulation may lie on microRNAs (miRNAs), which are endogenously expressed small non-coding RNAs of 18-24 nucleotides in length that regulate gene expression on the posttranscriptional level [ 14 ]. (biomedcentral.com)
It had been discovered that miR-31 could suppress reporter gene activity in these cells, whereas mutants plasmids showed zero noticeable adjustments. (nos-nop.org)
Gene expression-based prognostic signatures have a significant effect on predicting the survival of patients with malignant tumors, such as non-small cell lung cancer [ 7 ] and pediatric acute myeloid leukemia [ 8 ]. (aging-us.com)
Gene expression, Kaplan-Meier survival, microRNA and transcription factor target analyses were performed with the University of Alabama Cancer (UALCAN) Database, Kaplan-Meier Plotter, LinkedOmics, WebGestalt and gene set enrichment analysis, respectively. (xiahepublishing.com)
Reference gene stabilities were calculated first by standard deviations of ΔCt values and then by geNorm and NormFinder analyses, taking into account the amplification efficiency of each microRNA primer set. (biomedcentral.com)
Even small changes in microRNA expression may therefore have implications for gene regulation in various physiological and pathophysiological states [ 1 , 6 ]. (biomedcentral.com)
The small nuclear RNA U6 is frequently used as a reference gene (RG) in such studies [ 20 ]. (biomedcentral.com)
Despite the well-known fact that primiRNAs are acting as non-protein-coding RNAs, the published data suggest that, in the plant genomes, some pri-miRNAs can also be found in polysomes, and the expression of these miRNA precursors may results in formation of micropeptides which may be involved in regulation of gene expression. (fortunepublish.com)
Combined with miRNAs-based transcriptome sequencing assay, it was found that Tanshinone IIA downregulated miR-125b level and upregulated its target gene STARD13 (StAR-related lipid transfer protein 13) level, thus inactivating the miR-125b/STARD13 axis, which had been previously confirmed to promote breast cancer progression. (biomedcentral.com)
Significant downregulation of beta-defensin1 gene (DEFB1) expression was observed when human bronchial epithelial cells (HBEpCs) were exposed to IAV. (cdc.gov)
NRAV, a Long Noncoding RNA, Modulates Antiviral Responses through Suppression of Interferon-Stimulated Gene Transcription. (aksomics.com)
Mature miRNAs are generated from sequential processing of primary miRNA transcripts by Drosha and Dicer, then serve as posttranscriptional regulators of gene expression in certain biological events including carcinogenesis [ 10 ]. (biomedcentral.com)
The BIC gene is activated by promoter insertion at a retroviral integration site on chromosome 21q21 in B cell lymphomas induced by avian leukosis virus. (biomedcentral.com)
Also, since BCL6 interacts with several co-repressor complexes to inhibit transcription, and its gene is frequently trans-located and hyper-mutated in diffuse large B cell lymphoma (DLBCL), miR-155 acts to enhance transcription and contribute to the pathogenesis of DLBCL. (biomedcentral.com)

A large number of studies have demonstrated that the synergistic collaboration of a number of microRNAs (miRNAs), their growth factors and their downstream agents is required for the initiation and completion of pathogenesis in the liver. (wjgnet.com)
Non-coding RNAs (ncRNAs) are commonly dysregulated in disease pathogenesis, and their role in cancer has been well-documented. (drtaheriazam.com)
The field of non-coding RNA (ncRNA) has made significant progress in understanding the pathogenesis of diseases and has broadened our knowledge towards their targeting, especially in cancer therapy. (drtaheriazam.com)
By now, miRNAs have been well-coined in pathogenesis and development of cancer. (drtaheriazam.com)
Instead we use an in vitro HBV-replicating liver cell model to investigate the biological role of microRNAs with a possible influence on the pathogenesis of CHB in children. (biomedcentral.com)
Emerging evidence suggests that the long noncoding RNA (lncRNA) growth arrest special 5 (GAS5) plays crucial roles in the pathogenesis of ischemic stroke (IS). (hindawi.com)

Thus, the pivotal roles of miR-455 in cell viability and metastasis in HCC still needed to be explored. (hindawi.com)
However, advanced stage hepatic carcinoma lacks effective treatment and intervention, and it is prone to metastasis at the early stages ( 4 ). (spandidos-publications.com)
SOX2 has capacity of increasing growth and metastasis of cancer cells. (drtaheriazam.com)
This protein enhances cell motility, invasion and metastasis of cancer cells. (cancer-genetics.org)
Long Noncoding RNA Activated by TGF-β Promotes the Invasion-Metastasis Cascade in Hepatocellular Carcinoma. (aksomics.com)

Hepatocellular carcinoma (HCC) has been regarded as the fifth most common cancer worldwide with a low prognosis. (hindawi.com)
Clinically, there is an urgent need to identify the effective molecular biological markers for the treatment and prognosis of hepatic carcinoma. (spandidos-publications.com)
In this way, the discovery of the tumor-associated protein lncRNA and elucidation of its associated mechanisms will provide new insights in terms of the prognosis and treatment of hepatic carcinoma. (spandidos-publications.com)
Long noncoding RNA GAPLINC regulates CD44-dependent cell invasiveness and associates with poor prognosis of gastric cancer. (aksomics.com)

In our research, microRNAs (miRNAs) binding to the genome sequences of severe acute respiratory syndrome coronavirus (SARS-CoV), Middle East respiratory-related coronavirus (MERS-CoV), and SARS-CoV-2 were identified by bioinformatic tools. (molcells.org)

RAB18 reversed partial roles of miR-455 on cell viability and invasion in HCC. (hindawi.com)
Most reports found that multiple miRNAs that include miR-548a, miR-1246, miR-632, and miR-5692a played pivotal roles in HCC [ 7 - 10 ]. (hindawi.com)
Our study uncovered a novel molecular mechanism of lncRNA SNHG4 in driving prostate cancer progression and enzalutamide resistance, revealing the critical roles and therapeutic potential of RREB1, SNHG4, RRM2 and let-7 miRNAs in anticancer therapy. (biomedcentral.com)
Tanshinone II A is an effective component extracted from Salvia miltiorrhiza and the roles of Tanshinone IIA in regulating the stemness of tumor cells remain unclear. (biomedcentral.com)

lncRNA XIST accelerates hepatic carcinoma progression by targeting the miR‑320a/PIK3CA axis, which might provide the theoretical basis for the potential targeted therapy of hepatic carcinomas. (spandidos-publications.com)
EGFRvIII impacts both cell-intrinsic and non-cell autonomous aspects of GBM progression, including cell invasion, angiogenesis and modulation of the tumor microenvironment. (cancer-genetics.org)
The quality and quantity changes of circulating RNAs, mainly miRNA, are broadly investigated and associated with the initiation and progression of cancer [ 5 ]. (scientificarchives.com)

Functional enrichment analysis revealed SNHG4 involvement with regulation of ribosomal RNA synthesis and the RNA processing and surveillance pathway. (xiahepublishing.com)
Combinatorial regulation is a feature of miRNA regulation in animals. (microrna1.com)

Identifcation of differentially expressed long non-coding RNAs in CD4+ T cells response to latent tuberculosis infection. (aksomics.com)
Evaluation of the most differentially expressed miRNAs showed that miR-132, miR-367b, miR-410, and miR-152 were decreased in more aggressive cells, and this was reversed after treatment of the cells with 5-aza-2′-deoxycytidine. (scite.ai)

The aim of the present review is to evaluate the role of ncRNAs in regulating autophagy in gastrointestinal tumors. (drtaheriazam.com)
Recently, noncoding RNAs (ncRNAs) have been reported to regulate protein expression, and it is definite that PTEN expression is controlled by ncRNAs epigenetically or posttranscriptionally as well. (biomedcentral.com)

Luciferase reporter assay and RNA pull-down assay were carried out to detect the interaction between miR-383 and LncRNA PTTG3P. (biomedcentral.com)
Luciferase reporter assay and RNA immunoprecipitation assay were performed to verify the RNA-RNA interactions. (biomedcentral.com)

Endothelial progenitor cells (EPCs) play a fundamental role in post-natal vascular repair, yet EPCs from different anatomic locations possess unique biological properties. (biomedcentral.com)
Like intercellular RNAs, circulating RNAs participate in numerous biological process and express aberrantly under abnormal or pathological status. (scientificarchives.com)

Here we analyzed the impact of EGFRvIII on the profile of glioma EVs using isogenic tumor cell lines, in which this oncogene exhibits a strong transforming activity. (cancer-genetics.org)

Accordingly, glutamine consumption, α-KG production, and ATP levels were dramatically decreased by TUG1 knockdown in ICC cells, and this effect was reversed by miR-145 inhibition. (oncotarget.com)
MicroRNA-181 expression could be directly induced upon an activation of Wnt/β-catenin signaling, which includes Wnt10B overexpression, inhibition of GSK3β signaling by LiCl, or forced expression of β-catenin/Tcf4. (biomedcentral.com)
For example, Cirs-7 is derived from a highly conserved single exon, also known as antisense transcription of cerebellar degeneration-related protein 1 (CDR1as), which can regulate miR-7 through adsorption, thus reducing the inhibition of miR-7 on its target [ 19 ]. (biomedcentral.com)

In addition to RNA-binding activity, this protein interacts directly and highly specifically with subunit 2 of the splicing factor 3B. (nih.gov)
miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. (cancerindex.org)
As demonstrated in Fig. ?Fig.2B,2B, knockdown augmented HDAC2 and CDK2 protein expressions in SNU-449 and SKHep-1 cells, whereas co-transfection of miR-31 mimics attenuated knockdown effect on the same cells. (nos-nop.org)
PTEN also dephosphorylates Src homology 2-containing protein (Shc) directly, and inhibits the activation of Shc/Raf/ERK1/2 (extracellular signal-regulated kinase) signaling cascade. (biomedcentral.com)
Binding of this protein to CXCR3 results in pleiotropic effects, including stimulation of monocytes, natural killer and T-cell migration, and modulation of adhesion molecule expression. (cancerindex.org)
And a recent study demonstrates that Tanshinone IIA restrains migratory and invasive ability of cervix carcinoma stem cells by suppressing the transcriptional activity of YAP (Yes-associated protein) [ 11 ]. (biomedcentral.com)
The study has previously revealed that miR-125b faciliates cell migratory ability breast cancer via targeting STARD13 (StAR-related lipid transfer protein 13) [ 20 ]. (biomedcentral.com)
Alcohol www.selleckchem.com/products/17-AAG(Geldanamycin).html dehydrogenase (Adh) and cytochrome p450 enzyme 2E1 (Cyp2E1) are the two main hepatic enzymes to metabolize alcohol and to convert alcohol to acetaldehyde.29 Microsomal Cyp2E1 protein was similarly up-regulated in the ethanol-treated groups (Fig. 3B). (rafsignaling.com)
Based on our identified miRNA-regulated molecular machinery, an inhibitor of PDK1/Akt BX-912, an anthracycline antibiotic daunorubicin, and a multi-targeted protein kinase inhibitor midostaurin were discovered as potential repositioning drugs for treating lung cancer. (cdc.gov)
The viral spike protein is essential for cell receptor binding, cell entry, and viral infection. (molcells.org)
RNA-binding-protein-driven circularisation. (biomedcentral.com)

microRNAs have emerged as master regulators of stem cell lineage differentiation and angiogenesis [ 14 ]. (biomedcentral.com)
Since then, several microproteins have been functionally characterized, which may act, for example, as signals promoting cell migration and differentiation of human cells [5, 7]. (fortunepublish.com)

Hence, it is of great importance to investigate the mechanisms that induce enzalutamide resistance in prostate cancer cells. (biomedcentral.com)
MiRNAs-based transcriptome sequencing and data analysis, online dataset analysis, luciferase reporter assay combined with rescuing experiments were constructed to explore the underlying mechanisms. (biomedcentral.com)
It synthesizes information from ten recent papers on miR-155, and includes an analysis and discussion of its association with cancer, interactions with other miRNAs, mechanisms of action, and the most promising available treatment options. (biomedcentral.com)

The previous work has shown that Tanshinone IIA could ameliorate inflammation microenvironment in colorectal cancer via repressing miRNA-155 expression ( 8 ) and exert an anti-angiogenic effect in vascular endothelial cells via inhibiting the VEGF/VEGFR2 pathway [ 9 ]. (biomedcentral.com)

Others attempt to model more realistic cellular scenarios, where multiple RNA targets are affecting multiple miRNAs and where each target pair is co-regulated by multiple miRNA species. (wikipedia.org)

Hepatocellular carcinoma (HCC) is a malignant cancer with an observable heterogeneity and microRNAs are functionally associated with the tumorigenesis of HCC. (biomedcentral.com)
Current debates in the field include the importance of miRNAs in general and their utility as targets in preventing tumorigenesis (Blood 119:513-520, 2012). (biomedcentral.com)
Those miRNAs that lead to tumorigenesis and cancer are classed as oncomiRs. (biomedcentral.com)

Interestingly, ZNFX1 antisense RNA 1 (ZFAS1) could abolish its tumor suppressor effect by binding microRNA (miR)-150, while miR-150 could inhibit the invasion of hepatic carcinoma cells by inhibiting zinc finger E-box binding homeobox 1 (ZEB1) and matrix metalloproteinases (MMP)14 and MMP16 ( 10 ). (spandidos-publications.com)

Taken together, our results demonstrate that microRNA-181s are transcriptionally activated by the Wnt/beta-catenin signaling pathway in HCC. (biomedcentral.com)

For example, a small peptide that is encoded by lncRNA HOXB-AS3 inhibits oncogenesis by regulating alternative splicing and metabolic reprogramming of colon cancer cells [5-7, 17]. (fortunepublish.com)

Long non-coding RNA MRUL promotes ABCB1 expression in multidrug-resistant gastric cancer cell sublines. (aksomics.com)
Microarray Expression Profile Analysis of Long Non-coding RNAs in Human Gastric Cardiac Adenocarcinoma. (aksomics.com)

Most notably, it has been challenged by a group of researchers that performed a quantitative assessment of two miRNA families (highly and lowly expressed) and their binding sites in liver- and embryonic stem cells as described below. (wikipedia.org)
Hepatoma cell lines (SUN449), hepatoblastoma cell line (HepG2, Huh‑6), liver cancer cell line (HepG2) and transformed human liver epithelial‑2 cells (THLE‑2) were used in the present study. (spandidos-publications.com)
At present, surgical resection and liver transplantation are effective treatments for early hepatic carcinoma ( 2 ). (spandidos-publications.com)
The manifestation of miR-31 was normalized to U6 snRNA (*test) (D) The qRT-PCR analysis of miR-31 for hepatocellular carcinoma cell lines (n=7) and liver normal cell lines (n=2) (**test). (nos-nop.org)
miRNAs are thought to exert a profound effect on almost every aspect of liver biology and pathology. (wjgnet.com)
Hepatocellular carcinoma (HCC), which represents about 85-90% of cases of primary liver cancer, is the fourth most common malignancy and the third leading cause of tumor-related deaths in China. (xiahepublishing.com)
Hepatocellular carcinoma (HCC) is a primary liver cancer with high incidence rate, especially in regions with high prevalence of viral hepatitis infection. (drtaheriazam.com)
Nonalcoholic fatty liver disease (NAFLD) encompasses a broad spectrum of pathological hepatic conditions ranging from simple steatosis to nonalcoholic steatohepatitis (NASH), which may predispose to liver cirrhosis and hepatocellular carcinoma (HCC). (encyclopedia.pub)
It encompasses a wide spectrum of hepatic conditions ranging from simple steatosis, a benign manifestation characterized by lipid accumulation exceeding 5% of liver weight excluding other etiological causes, to a more severe form, such as nonalcoholic steatohepatitis (NASH), fibrosis, cirrhosis and hepatocellular carcinoma (HCC) [ 1 ] [ 2 ] . (encyclopedia.pub)

In addition, seven putative β-catenin/Tcf4 binding sites were identified in the promoter region of the microRNA-181a-2 and microRNA-181b-2 transcripts. (biomedcentral.com)

The aim of the present study was to reveal the new molecular mechanism of long non‑coding (lnc)RNA XIST in the development of hepatic carcinoma. (spandidos-publications.com)
A total of 69 patients with hepatic carcinoma were included. (spandidos-publications.com)
A total 3 short hairpin RNA (sh)‑lncRNA XIST sequences, overexpression vector (oe)‑lncRNA XIST, microRNA (miR)‑320a mimic, miR‑320a inhibitor, PIK3CA inhibitor, and their corresponding controls were transfected in hepatic carcinoma cells. (spandidos-publications.com)
Hepatic carcinomas are among the most common malignant tumors in China, and cause serious threat to individuals' health ( 1 ). (spandidos-publications.com)
The fatality rate of Hepatic carcinoma ranks second among malignant tumors. (spandidos-publications.com)
With advancements in research on various surgical and drug treatments, the 5-year survival rate of hepatic carcinoma patients has increased ( 3 ). (spandidos-publications.com)
Further, Zamani et al ( 9 ) investigated the epigenetic effects of solute carrier family 25 member 19 in hepatic carcinoma and confirmed that DNA methylation in the functional region of maternally expressed 3 (MEG3) was associated with its loss of expression in hepatic carcinoma. (spandidos-publications.com)
H19 was also found to induce p-glycoprotein expression and MDR1-associated drug resistance in hepatic carcinoma cells by regulating the demethylation of the MDR1 promoter ( 11 ). (spandidos-publications.com)
We recently identified that EpCAM (CD326)-positive cells isolated from alpha-fetoprotein (AFP)-positive HCC samples are hepatic cancer stem cells (HepCSCs). (biomedcentral.com)
We recently found that isolated HCC cells using a cell surface marker EpCAM (CD326) from alpha-fetoprotein (AFP) positive HCC cases are hepatic cancer stem cells (HepCSCs), where an activation of Wnt/β-catenin signaling is a major feature [ 5 ]. (biomedcentral.com)
Obesity, nutrition and environmental factors might alter intestinal permeability producing a favorable micro-environment for bacterial overgrowth, mucosal inflammation and translocation of both invasive pathogens and harmful byproducts, which, in turn, influence hepatic fat composition and exacerbated pro-inflammatory and fibrotic processes. (encyclopedia.pub)

In vitro and in vivo experiments showed that SNHG4 overexpression markedly enhanced cell resistance to enzalutamide. (biomedcentral.com)
Notably, miR-125b overexpression enhanced the stemness of breast cancer cells, and miR-125b overexpression or STARD13 knockdown impaired the inhibitory effects of Tanshinone IIA on the stemness of breast cancer cells. (biomedcentral.com)

CASC11 promotes aggressiveness of prostate cancer cells through miR-145/IGF1R axis. (istanbul.edu.tr)
miR-132 was found to be involved in diseases such as epilepsy ( 8 ), prostate cancer ( 9 ) and hepatocellular carcinoma ( 10 ). (spandidos-publications.com)
Herein, we probed the miRNA expression profile of novel AA and CA derived prostate cancer cell lines. (scite.ai)

Similarly, the miRNA signature shows predictive value in adults with T-cell lymphoblastic lymphoma [ 9 ], and long noncoding RNA shows predictive value in adults with localized clear cell renal cell carcinoma [ 10 ]. (aging-us.com)
It turned out that four peptides, encoded by a number of long non-coding RNAs, have a length of 11 to 32 residues and are necessary for embryonic development of flies [13, 14]. (fortunepublish.com)
A distinct set of long non-coding RNAs in childhood MLL-rearranged acute lymphoblastic leukemia: biology and epigenetic target. (aksomics.com)
Expression profile of long noncoding RNAs in cartilage from knee osteoarthritis patients. (aksomics.com)
Aberrantly expressed long noncoding RNAs in human intervertebral disc degeneration: a microarray related study. (aksomics.com)
Expression Pattern of Long Non-coding RNAs in Renal Cell Carcinoma Revealed by Microarray. (aksomics.com)
The Long Noncoding RNA Expression Profile of Hepatocellular Carcinoma Identified by Microarray Analysis. (aksomics.com)

MicroRNAs (miRNAs, miRs) are a class of small, non-coding, endogenous and functional RNAs. (biomedcentral.com)
While there is some controversy surrounding recent research that claims that miR-155 may display anti-oncogenic or pro-immunological benefits (Cell Rep 2:1697-1709, 2012), most research seems to point to the importance of anti-miRs, with anti-miR-155 in particular, for cancer therapy. (biomedcentral.com)
microRNAs (miRs) are endogenous small noncoding RNAs that are aberrantly expressed in various carcinomas. (scite.ai)

Cell viability and invasion were measured by CCK8 and Transwell assays. (hindawi.com)
However, miR-455 promoted cell invasion and migration in triple-negative breast cancer [ 14 ]. (hindawi.com)
Cell invasion and migration were detected by Transwell assays. (spandidos-publications.com)
Transwell migration and invasion assays were used to examine cell migration and invasion abilities. (biomedcentral.com)
CircRNA_104889 promotes lung adenocarcinoma cell invasion via sponging miR4458. (istanbul.edu.tr)

For a cooperative effect to be considered two adjacent MREs, however, have to be of miRNA families that are expressed high enough to actively repress targets and to be less than 58 nucleotides apart. (wikipedia.org)
To test whether miR-181s were transcriptional targets of Wnt/β-catenin, we first examined the expression of β-catenin and miR-181s in five different HCC cell lines. (biomedcentral.com)

Sex cord-stromal tumors are groups of tumors composed of granulosa cells, theca cells, Sertoli cells, Leydig cells, and fibroblasts of stromal origin, singly or in various combinations. (medscape.com)
A worldwide database analysis revealed that younger age, smaller tumor size, early stage, and granulosa cell tumor histologic type appear to be independent prognostic factors for improved survival in patients with malignant sex cord-stromal tumors who undergo lymph node dissection. (medscape.com)

The lncRNA XIST negatively targeted miR‑320a, and miR‑320a negatively regulated the expression of PIK3CA. (spandidos-publications.com)
miR‑132 directly targeted SIRT1 and negatively regulated the expression of SIRT1. (spandidos-publications.com)

MiRNAs are reported to involved in multiple cellular processes [ 14 ]. (biomedcentral.com)
It is involved in cellular processes including cell adhesion and may regulate integrin trafficking and/or function. (cancer-genetics.org)
Sirtuin 1 (SIRT1), a member of the sirtuin family, regulates the cellular ageing process, participates in metabolic diseases, such as diabetes, and is related to cancer development ( 13 ). (spandidos-publications.com)
Furthermore, individual variability of these salivary RNA species was highly similar to those in other body fluids or cellular samples, despite the direct exposure of saliva to environmental impacts. (scite.ai)

The present study was conducted to identify suitable RGs for microRNA expression studies in a human HCC-derived cell line (HepG2 tet-on), with and without HBV replication, after transfection of HBV expression vectors. (biomedcentral.com)
48 h after transfection with the indicated RNA duplex, cells were subjected to Western blot analysis. (biomedcentral.com)
DEFB1 expression after transfection with two micro RNAs (miRNAs), hsa-miR-186-5p and hsa-miR-340-5p, provided evidence that DEFB1 expression could be modulated by these miRNAs and hsa-miR-186-5p had a higher binding efficiency with DEFB1. (cdc.gov)

Thus, umbilical cord blood EPCs hold great therapeutic potential for cell therapy and vascular engineering. (biomedcentral.com)

To dissect the role of PMN in this context, we pretreated rats with Repertaxin (Rep), a small molecule inhibitor of CXCR1 and CXCR2, to block recruitment and activation of PMN by CXCL1 or CXCL2 after cell transplantation. (p450inhibitors.com)
MicroRNAs (miRNAs) are a class of small non-coding RNA, 20-22 nucleotides in length. (molcells.org)

Colony formation would healing and transwell assays were used to detect cell function. (biomedcentral.com)

Some describe the kinetics of target-miRNA-target interactions, where changes in the expression of one target species sequester one miRNA species and lead to changes in the dysregulation of the other target species. (wikipedia.org)
Altered expression levels of specific microRNAs are associated with hepatitis B virus infection and hepatocellular carcinoma. (biomedcentral.com)
Tanshinone IIA attenuated the stemness of breast cancer cells, evident by downregulating the expression of stemness markers, hindering the capacity of spheroid formation, decreasing the CD24 − /CD44 + sub-population in a concentration-dependent manner and reducing the tumor-initiating ability of breast cancer cells. (biomedcentral.com)
The expression patterns of those miRNAs were examined in hamster lung samples infected by SARS-CoV-2. (molcells.org)
In this study, we found that the SCARA5 expression was lower in CAL-27 and SCC-9 cells than that in human normal oral epithelial keratinocytes. (degruyter.com)
In addition, SCARA5 repressed OSCC cell epithelial-mesenchymal transformation (EMT), evidenced by increased E-cadherin expression and reduced N-cadherin expression. (degruyter.com)
Analysis of Altered Microarray Expression Profiles in Proximal Renal Tubular Cells in Response to Calcium Oxalate Monohydrate Crystal Adhesion: Implications for Kidney Stone Disease. (aksomics.com)
EpCAM + AFP + HepCSCs have an activated Wnt/β-catenin signaling with a parallel increased expression of all four microRNA-181 family members. (biomedcentral.com)
Using both western blot and quantitative reverse transcriptase-PCR analyses, we found that the expression of all four microRNA-181 family members was positively correlated with β-catenin expression in HCC cell lines. (biomedcentral.com)
Moreover, microRNA-181 expression was inhibited upon an inactivation of Wnt/β-catenin signaling by an induction of adenomatosis polyposis coli (APC) expression or silencing β-catenin via RNA interference. (biomedcentral.com)
By comparative analysis of >90 RNA-Seq data sets of different origins, we observed that piRNAs were surprisingly abundant in CFS compared with other body fluid or intracellular samples, with expression levels in CFS comparable to those found in embryonic stem cells and skin cells. (scite.ai)
Conversely, miRNA expression profiles in CFS were highly similar to those in serum and cerebrospinal fluid. (scite.ai)
Of the miRNAs we assayed, miR-152 demonstrated the most significant reversal of expression, an effect consistent for both LNCaP and PC-3 cell lines. (scite.ai)
miRNA expression in African American compared to Caucasian PCa patients has not been widely explored. (scite.ai)

SOX2 promotes stemness of tumor cells and increases the number of cancer stem cells. (drtaheriazam.com)

Objective: Acute myeloid leukemia (AML) is a lethal type of cancer associated with dysregulation of progenitor hematopoietic stem cell behavior and its incidence is, unfortunately, increasing. (istanbul.edu.tr)

Anatomy10

Organisms7

Diseases45

Chemicals and Drugs75

Analytical, Diagnostic and Therapeutic Techniques and Equipment39

Phenomena and Processes44

Disciplines and Occupations1

Information Science4

Health Care11

Genes26

MRNA22

LncRNAs15

Apoptosis21

Proliferation12

Proteins18

CircRNAs10

Competitive endogenous1

Biomarkers8

MRNAs3

CeRNA9

Pathways5

Molecules2

Tissues14

Sponges1

Squamous cell carc1

CeRNAs1

Nasopharyngeal carcinoma1

Circular10

Gene15

Pathogenesis6

Metastasis5

Prognosis4

Bioinformatic1

Roles4

Progression3

Regulation2

Differentially expressed2

NcRNAs2

Luciferase reporter assay and RNA2

Biological2

Oncogene1

Inhibition3

Protein11

Differentiation2

Mechanisms3

Colorectal1

Multiple miRNAs1

Tumorigenesis3

Tumor suppressor effect1

Pathway1

Inhibits1

Gastric2

Liver9

Transcripts1

Hepatic11

Overexpression2

Prostate3

Long7

MiRs3

Invasion5

Targets2

Tumors2

Negatively regulated the expression2

Cellular4

Transfection3

Therapeutic1

Small2

Assays1

Expression14

Stemness of tumor cells1

Acute myeloid1