• Addgene: High-fidelity, efficient, and reversible labeling of endogenous proteins using CRISPR-based designer exon insertion. (addgene.org)
  • Chinese hamster ovary (CHO) cells are the most commonly used mammalian cell expression system for the industrial production of biotherapeutic recombinant proteins. (kent.ac.uk)
  • Mammalian expression systems are a popular choice for the production of complex biopharmaceuticals due to their ability to correctly fold and assemble recombinant proteins and to carry out necessary human-like post-translational modifications. (kent.ac.uk)
  • With over 35 years of providing the best recombinant proteins to the scientific community, R&D Systems continues to lead the industry in quality, activity, and purity. (rndsystems.com)
  • CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. (rndsystems.com)
  • MultiShot StripWell BL21 Star (DE3) chemically competent E. coli cells are cloning competent cells designed for applications that require high-level expression of non-toxic recombinant proteins from low copy number, T7 promoter-based expression systems. (thermofisher.com)
  • The BL21 (DE3) E. coli strain is one of the most popular host strains to produce recombinant proteins and adapted in research or industrial applications. (thermofisher.com)
  • A powerful tool to understand the molecular mechanism of small silencing RNA production by Dicers is an in vitro RNA processing assay using recombinant Dicer proteins. (nih.gov)
  • Such biochemical analyses have elucidated the substrate specificities and kinetics of Dicers, the mechanism by which the length of small RNAs produced by Dicers is determined, and the effects of Dicer-partner proteins and endogenous small molecules such as ATP and inorganic phosphate on small RNA production by Dicers, among others. (nih.gov)
  • Here, we describe methods for in vitro small RNA production assay using recombinant human and Drosophila Dicer proteins. (nih.gov)
  • Among subjects of all racial ancestries enrolled in HIGS who reported early use of recombinant products (N = 223), mismatch in endogenous haplotype and the FVIII proteins constituting the products used did not confer greater risk for inhibitor development. (lu.se)
  • Here we developed recombinant E3L proteins to detect dsRNA and Z-dsRNA generated from viral infections or endogenous cellular mutations. (bvsalud.org)
  • These vectors have a number of appealing features including the expression by using the machinery of the host cell instead of depending abilities to ef®ciently transduce cells in the central nervous system, on recombinant regulatory proteins. (lu.se)
  • Recombinant elastin-based proteins (ELPs) are used in applications that include therapeutics, drug delivery, and tissue engineering due to their biocompatibility and unique ability to undergo simple coacervation. (bvsalud.org)
  • i>The chassis of choice for production and secretion of the Histamine Binding Protein is  Staphyloccocus Epidermidis, an organism endogenous to the human nasal flora. (igem.org)
  • Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. (rndsystems.com)
  • In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. (rndsystems.com)
  • The 332 amino acid recombinant protein has a predicted molecular mass of approximately 36.7 kD. (biolegend.com)
  • 1. Dilute the recombinant mouse Cathepsin B to 10 μg/ml in activation buffer (25 mM MES, 1 mM DTT, pH 5.0) and incubate the protein at 25°C for 3 min. (biolegend.com)
  • MultiShot StripWell BL21 Star (DE3) Competent Cells offer enhanced mRNA stability due to a mutation in the RNaseE gene ( rne 131) that reduces levels of endogenous RNases and mRNA degradation, thereby increasing the stability of mRNA transcripts and increasing protein yield. (thermofisher.com)
  • Recombinant Human Neuroserpin is a 44.8 kDa non-glycosylated protein containing 395 amino-acid residues. (peprotech.com)
  • We demonstrate that the E3L recombinant protein specifically detects A-dsRNA generated from +ssRNA viruses but not-RNA viruses. (bvsalud.org)
  • We observe that among various virus infections assayed, only the influenza A virus generates Z-RNA that can be detected by anti-Z-NA antibody but not by the E3L recombinant protein containing the Z-DNA domain. (bvsalud.org)
  • The E3L recombinant protein can also detect endogenous dsRNA in PNPT1 or SUV3L1 knockout cells. (bvsalud.org)
  • We engineered recombinant vaccinia virus E3L protein that can detect A-dsRNA generated during infection with a positive-sense RNA genome virus but not a negative-sense RNA genome virus. (bvsalud.org)
  • Infection with influenza A virus generates Z-RNA that can be detected with an anti-z-antibody but not the E3L recombinant protein. (bvsalud.org)
  • The E3L recombinant protein also detects endogenous dsRNA in PNPT1 or SUV3L knockout cells. (bvsalud.org)
  • Although these promoters provide high recombinant gene expression, this is not always favourable as constitutive transgene over expression can ultimately lead to cellular stress. (kent.ac.uk)
  • Endogenous and synthetic CHO putative promoter sequences were investigated for their abilities to drive reporter and recombinant gene expression both in stable and transient systems. (kent.ac.uk)
  • Indeed, the endogenous CHO promoter sequence was shown to achieve product titers of up to 3-fold greater than those from the commonly used CMV promoter with enhancer in CHO cells, demonstrating the potential for endogenous promoters to replace typically used viral promoters for recombinant gene expression. (kent.ac.uk)
  • In summary, this study has identified an endogenous CHO promoter sequence able to drive IgG expression beyond that of current widely used viral promoters and has generated additional promoters exhibiting a range of abilities to drive recombinant gene expression to varying amounts that could be applied to cell engineering approaches in the future. (kent.ac.uk)
  • Using gene-specific markers, we validate the presence of both genes on a single recombinant alien segment developed in wheat. (nature.com)
  • However, as there is no current Pgt race known to be virulent to either resistance gene, it was not possible to unambiguously determine whether the recombinant introgression carried a single or both genes. (nature.com)
  • Intravesical gene therapy might achieve sustained high levels of IFNα by inducing endogenous production by host cells. (medpagetoday.com)
  • the endogenous cyclin E gene is activated following expression of the E 2 F 1 product encoded by a recombinant. (wikigenes.org)
  • Ancestral background, specifically African descent, confers higher risk for development of inhibitory antibodies to factor VIII (FVIII) in haemophilia A. It has been suggested that differences in the distribution of FVIII gene (F8) haplotypes, and mismatch between endogenous F8 haplotypes and those comprising products used for treatment could contribute to risk. (lu.se)
  • Using toxin-induced lesions we investigated to what extent transgene expression could be regulated in accordance with the activation of the endogenous GFAP gene. (lu.se)
  • The vast majority of these cells did not divide, suggesting that the transgene was indeed regulated in a similar fashion as the endogenous GFAP gene. (lu.se)
  • An aspect of gene delivery to the brain that is currently being regulate the endogenous GFAP expression in a lesioned brain. (lu.se)
  • A second research question regarding risk related to mismatch in endogenous F8 haplotype and recombinant FVIII products used for treatment was addressed. (lu.se)
  • 3. for measurement of factor VIII (FVIII) and factor IX (FIX) inhibitors that includes a 30minute preanalytical heat treatment (PHT) step to remove endogenous and infused FVIII or FIX. (cdc.gov)
  • Cystatin C has been identified as an endogenous CTSB inhibitor. (biolegend.com)
  • In addition to its role in the endogenous control of erythropoiesis, recombinant human erythropoietin (rh-EPO) has been shown to exert tissue protective properties in various experimental models. (nih.gov)
  • Anemia in pregnancy and the puerperium should be treated according to a staged regimen, administering either iron alone or in combination with an off-label use of human recombinant erythropoietin in selected patients. (karger.com)
  • Clone G146-254 has been shown to recognize in vitro translated, recombinant bacterially expressed, and endogenous B cell Fli-1. (bdbiosciences.com)
  • In vitro studies demonstrate that exogenous recombinant Gal3 is internalised by neuronal cell lines and primary neurons where it interacts with endogenous αSyn fibrils. (lu.se)
  • Turoctocog alfa is a recombinant human coagulation factor VIII and once activated by thrombin cleavage the resulting rFVIIIa has the same structure as endogenous FVIIIa. (janusinfo.se)
  • 1 μg/lane of Recombinant Human Fc gamma RIIB/C (CD32b/c) was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing multiple bands at 27-35 kDa. (rndsystems.com)
  • Recombinant Human Wnt-3a (Catalog # 5036-WN) induces alkaline phosphatase production by the MC3T3-E1 mouse preosteoblast cell line. (novusbio.com)
  • Enhanze™ technology is a proprietary drug delivery platform using Halozyme's first approved enzyme, recombinant human hyaluronidase or rHuPH20. (prnewswire.com)
  • Final Report Summary - ALPHA-MAN (Clinical development of Enzyme Replacement Therapy in alpha-Mannosidosis patients using recombinant human enzyme. (europa.eu)
  • Since pharmaceutical interest in this disease is low, two EU-supported projects (EURAMAN and HUE-MAN) within the 5th and 6th framework program, respectively have worked towards developing the recombinant human enzyme (rhLAMAN) as a therapeutic agent for patients suffering from alpha-Mannosidosis. (europa.eu)
  • The authors have been investigating the metabolism of a number of agrochemicals by human liver microsomes and recombinant CYP isoforms and have recently embarked on studies of the induction of XMEs in human hepatocytes. (cdc.gov)
  • The FeLV-Pco virus health, wildlife managers released 8 female Texas pumas was closely related to the domestic cat exogenous FeLV-A into southern Florida to increase genetic variation and ame- subgroup in lacking recombinant segments derived from liorate the physiologic effects of inbreeding. (cdc.gov)
  • Chlorpyrifos and other phosphorothioates are potent inhibitors of the CYP-dependent metabolism of both endogenous substrates, such as testosterone and estradiol, and exogenous substrates, such as carbaryl, presumably as a result of the interaction of highly reactive sulfur, released during the oxidative desulfuration reaction, with the heme iron of CYP. (cdc.gov)
  • 2. Dilute the recombinant mouse Cathepsin B to 0.05 μg/ml in assay buffer (25 mM MES, pH 5.0). (biolegend.com)
  • Sr26 and Sr61 are each validated by transgenic complementation using endogenous and/or heterologous promoter sequences. (nature.com)
  • This study reports on the investigation of endogenous CHO promoters as potential alternatives to the commonly used strong viral promoters such as SV40 and CMV. (kent.ac.uk)
  • Today, the most promising therapy for lysosomal storage disorders including alpha-Mannosidosis is Enzyme Replacement Therapy (ERT) where the respective enzyme lacking in the patient is produced by recombinant approaches and then introduced into the blood stream, from where it is internalized by the cells and reaches the lysosomes replacing the missing endogenous enzyme. (europa.eu)
  • Label-free detection, on the other hand, is proving to be a highly sensitive method of measurement for endogenous targets in live cell assays, and eliminates the need for tags, dyes or specialized reagents or engineered cells-reducing the resources required for assay development, simplifying assay design, minimizing liabilities created by the use of labels and enabling the use of native cells for greater biological relevance. (drugdiscoverynews.com)
  • The obtained results provide compelling evidence that higher level of hisADC transgene expression completely triggered the endogenous agmatine synthesis during H 2 O 2 injury thus protecting NIH3T3 cells against cytotoxicity. (elsevierpure.com)
  • The rationale led to development of the rAd-IFNα recombinant adenovirus with the syn 3 excipient to enhance adenoviral transduction of NMIBC cells. (medpagetoday.com)
  • Animal studies have shown that LAK cells are more effective against cancer cells than are the original endogenous T cells, presumably because of their greater number. (msdmanuals.com)
  • From these data, we conclude that neutrophil-mediated endogenous opioids warrant further investigation as a potential strategy for oral cancer pain treatment. (frontiersin.org)
  • Overall, 14 of 40 patients had high-grade recurrence-free survival (hgRFS) a year after treatment with recombinant adenovirus-linked interferon-alpha (rAd-IFNα/Syn3), including half of patients who had only papillary disease. (medpagetoday.com)
  • We found that recombinant G-CSF (rG-CSF, 5 μg/mouse, intraperitoneal) significantly increased circulating Ly6G + neutrophils in the blood of male and female mice within 24 h of administration. (frontiersin.org)
  • Genetic mutations can also lead to endogenous dsRNA accumulation. (bvsalud.org)
  • Upon generation of pools and mini pools stably producing IgG under the control of the various promoters, the CHO endogenous 2 kb promoter outperformed the synthetic candidates, the CMV with enhancer and an industrially relevant control promoter. (kent.ac.uk)
  • We infer that rG-CSF-induced Ly6G + neutrophils drive an endogenous analgesic mechanism. (frontiersin.org)
  • Michal Opas Department of Anatomy & Cell Biology, University of Toronto, Toronto, Ontario, CANADA Subcellular localization and function of endogenous and recombinant calreticulins. (bio.net)
  • Here, we investigated whether recombinant SVmab (rSVmab) binds to and blocks Nav1.7 similar to SVmab. (springer.com)
  • Its physiological role may be to transport or sequester an endogenous organic molecule other than Fe3+. (joplink.net)
  • Recombinant Elastin-Based Bioelastomers for Biomedical Applications. (bvsalud.org)
  • D1 receptor-mediated endogenous tPA upregulation contributes to blood-brain barrier injury after acute ischaemic stroke. (peprotech.com)