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  • cells
  • Using the EP cell culture system to bypass the developmental block at the endoderm stage, cell lines with mutations in one or both GATA6 alleles were differentiated into β-like cells. (upenn.edu)
  • specification
  • These data show that GATA6 plays a critical role in endoderm specification and β-like cell functionality in humans while in mice it is dispensable, highlighting the importance of studying a human system. (upenn.edu)
  • vertebrate
  • Among vertebrate GATA proteins, the GATA-4/5/6 subfamily regulates aspects of late endoderm development, but a role for GATA factors in establishing the endoderm is unknown. (pnas.org)
  • We exploited this activity of END-1 to demonstrate that establishment of the vertebrate endoderm, like that of invertebrate species, also appears to involve GATA transcriptional activity. (pnas.org)
  • By examining these effects in conjunction with those of Mixer- and Sox17β-activating and dominant inhibitory constructs, we further establish the likely relationships between GATA activity and these regulators in early development of the vertebrate endoderm. (pnas.org)
  • presumptive
  • ELT-2, a second gut-specific transcription factor expressed slightly later in the C. elegans endoderm, has two presumptive zinc fingers, one of which is similar to the GATA motif ( 5 ), and is required for maintenance of intestinal cells ( 6 ). (pnas.org)
  • tissue
  • Endoderm forms the epithelium-- a type of tissue in which the cells are tightly linked together to form sheets-- that lines the primitive gut. (asu.edu)
  • GATA
  • Similarly, mutations that eliminate the Drosophila GATA factor ABF/Serpent (dGATAb) result in the complete absence of the midgut, the only endoderm derivative in flies ( 7 , 8 ). (pnas.org)