Earlier two transferrin receptors in humans, transferrin receptor 1 and transferrin receptor 2 had been characterized and until recently cellular iron uptake was believed to occur chiefly via these two well documented transferrin receptors. (wikipedia.org)
Receptors, Transferrin" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (umassmed.edu)
This graph shows the total number of publications written about "Receptors, Transferrin" by people in this website by year, and whether "Receptors, Transferrin" was a major or minor topic of these publications. (umassmed.edu)
Below are the most recent publications written about "Receptors, Transferrin" by people in Profiles. (umassmed.edu)
Structural requirements and sequence motifs for polarized sorting and endocytosis of LDL and Fc receptors in MDCK cells. (wikidata.org)
The transferrin receptor in turn binds to adaptor proteins in the interior of the cell, triggering the formation of a clathrin cage around the bound transferrin receptors. (utah.edu)
Uptake of transferrin2
Knockdown of IRR inhibited glucose-induced uptake of transferrin, a marker of endocytosis, translocation of the guanine-nucleotide-exchange factor ARNO to the plasma membrane, and generation of phosphatidylinositol 3,4,5-trisphosphate (PIP 3 ). (fujita-hu.ac.jp)
Both of these methods resulted in reduced uptake of transferrin, a marker for clathrin-mediated endocytosis, and, under unstimulated conditions, reduced the uptake of the fluid-phase markers dextran and horseradish peroxidase (HRP). (elsevierpure.com)
Holo-transferrin3
Aprotinin transcytosis across bovine brain capillary endothelial cell (BBCEC) monolayers is at least 10-fold greater than that of holo-transferrin. (aspetjournals.org)
The results obtained with in vitro and in vivo models provided evidence for greater passage of aprotinin across the BBB than of holo-transferrin. (aspetjournals.org)
Supplied as a human holo-transferrin analog, rTransferrin binds specifically to the transferrin receptor thereby facilitating the bioavailability of iron to the cell. (genengnews.com)
Serum transferrin binds ferric ions in the bloodstream and transports them to cells, where they are released in a process involving receptor-mediated endocytosis. (rcsb.org)
Harding, C., Heuser, J. & Stahl, P. Endocytosis and intracellular processing of transferrin and colloidal gold-transferrin in rat reticulocytes: demonstration of a pathway for receptor shedding. (nature.com)
Moreover, the transferrin-receptor pathway is more efficient than free inorganic iron in the targeting of iron toward the mitochondria. (genengnews.com)
Using the Drosophila nephrocyte as a genetic screen platform, most genes involved in endocytosis and cell trafficking were screened, and the key components were identified of the cell trafficking pathway required for SD protein endocytosis and recycling. (sdbonline.org)
The SD protein endocytosis and recycling pathway was found to contain clathrin , dynamin , AP-2 complex, like-AP180 (Lap) , auxilin and Hsc70-4 (the endocytosis part) followed by Rab11 and the exocyst complex (the recycling part). (sdbonline.org)
All genes in this SD protein endocytosis and recycling pathway, as well as SD proteins themselves, are highly conserved from flies to humans. (sdbonline.org)
Thus, these results suggest that the SD proteins in human kidney undergo the same endocytosis and recycling pathway to maintain the filtration structure, and mutations in any genes in this pathway could lead to abnormal SD and renal diseases. (sdbonline.org)
Ingenuity pathway analysis indicated changes to clathrin-mediated endocytosis (CME) and CME-associated proteins, clathrin, Transferrin (TF), TF receptor and multiple Rabs, were identified as O-GlcNAcylation targets. (manchester.ac.uk)
Proteins1
SD proteins are known to undergo endocytosis and recycling to maintain the integrity of the filtration structure. (sdbonline.org)
Clathrin6
Transferrin uptake assay to measure Clathrin-mediated endocytosis in HIPCS derived neurons. (protocols.io)
Blocks clathrin-mediated endocytosis of transferrin when applied for 30 minutes to U2OS cells in culture (IC 50 = 7.0 μM). (abcam.com)
The selective effect of cytochalasin D on apical endocytosis was also demonstrated by the failure of the drug to reduce the uptake of 125I-labeled transferrin, which occurs by receptor-mediated endocytosis, via clathrin-coated pits, almost exclusively from the basolateral surface. (rupress.org)
In contrast to these well established effects of Rho and Rac on plasma membrane morphology and bulk pinocytosis, there has been no evidence for their involvement in the regulation of receptor-mediated endocytosis in clathrin-coated pits. (elsevierpure.com)
Clathrin is a coat protein that has been shown to function in receptor-mediated endocytosis events at the plasma membrane. (utah.edu)
The animation below shows the process of clathrin-mediated endocytosis. (utah.edu)
Apical2
Actin microfilaments play a critical role in endocytosis at the apical but not the basolateral surface of polarized epithelial cells. (rupress.org)
Treatment with cytochalasin D, a drug that acts by inducing the depolymerization of the actin cytoskeleton, selectively blocked endocytosis of membrane bound and fluid phase markers from the apical surface of polarized MDCK cells without affecting the uptake from the basolateral surface. (rupress.org)
Acidification2
Endocytosis of iron loaded transferrin/receptor complex leads, after acidification of the endosome, to the separation of iron and its diffusion out of the vesicle. (reactome.org)
They specifically bind iron-bearing transferrin, are endocytosed with its ligand and then returned to the cell surface where transferrin without its iron is released. (umassmed.edu)
EGFR2
3H]Thymidine uptake is clearly stimulated on KB cells by nanomolar concentrations of EGF and transferrin, whereas in Ara-C-treated cells [3H]thymidine uptake is not increased by EGF and transferrin under conditions where maximal EGFR and TrfR up-regulation occurs. (cnr.it)
The enhanced EGF and transferrin binding is paralleled by a twofold increase of in vitro targeting of Ara-C-treated KB and A549 cells with anti-EGFR 108.1 mAb and anti-TrfR OKT9 mAb. (cnr.it)
Conjugate1
Mass spectrometry-guided optimization and characterization of a biologically active transferrin-lysozyme model drug conjugate. (umassmed.edu)
Transferrin is also thought to be involved in cellular growth and differentiation activities. (genengnews.com)
In this review we summarize recent data regarding stress-induced changes in endocytosis and discuss how these changes might contribute to the cellular adaptation to stress in different systems. (cell-stress.com)
While certain aspects of stress-elicited adaptive programs such as the heat shock response or the oxidative stress response are very well studied, the contribution of other cellular pathways including endocytosis to cellular adaptation is still elusive. (cell-stress.com)
Selectively1
We next tested whether a specific spliced variant might selectively affect fluid-phase endocytosis. (elsevierpure.com)
Stimulating protein O-GlcNAcylation using glucosamine (2.5 mM) increased the rate of TF endocytosis by human placental cells (p = 0.02) and explants (p = 0.04). (manchester.ac.uk)
Mass spectrometry study of a transferrin-based protein drug reveals the key role of protein aggregation for successful oral delivery. (umassmed.edu)
Our recent study suggested that EPI64, a GTPase activating protein for Rab27a, contributes to the regulation of glucose-induced endocytosis, which is mediated by the GDP-bound form of Rab27a. (fujita-hu.ac.jp)
Cells6
Iron, insoluble as free Fe(3+) and toxic as free Fe(2+), is distributed through the body as Fe(3+) bound to transferrin (Tf) for delivery to cells by endocytosis of its complex with transferrin receptor (TfR). (rcsb.org)
Traditionally, transferrin has been supplied in the form of serum-derived human transferrin (hTf), bovine transferrin (bTf), or, more recently, with the move toward serum-free medium (SFM), inorganic iron salts have been used to supply iron to mammalian cells. (genengnews.com)
Endocytosis after insulin secretion plays a pivotal role in the regulation of insulin secretion in pancreatic β-cells. (fujita-hu.ac.jp)
Here we show that activated Rho and Rac inhibit transferrin-receptor-mediated endocytosis when expressed in intact cells. (elsevierpure.com)
Two classes of EGF-binding sites with a Kd of 0.055 nM and 2.3 nM respectively, and one class of transferrin-binding sites with a Kd of about 4 nM are detected on both untreated and Ara-C-treated KB cells. (cnr.it)
Endocytosis is the process by which cells are able to internalize membrane and extracellular materials through the formation of a vesicle. (utah.edu)
Transmembrane1
Endocytosis is a fundamental process involved in trafficking of various extracellular and transmembrane molecules from the cell surface to its interior. (mdpi.com)
Concentrations1
Low iron concentrations promote increased levels of transferrin receptor, to increase iron intake into the cell. (wikipedia.org)
Human2
To help define the steps in iron release, we have determined the three-dimensional structure of the iron-free (apo) form of the recombinant N-lobe half-molecule of human serum transferrin (ApoTfN) by X-ray crystallography. (rcsb.org)
It is a recombinant analog of human transferrin that has shown equivalence to serum-derived human transferrin (hTf) and superiority to bovine transferrin (bTf) in stimulating cell growth and productivity for a number of cell lines. (genengnews.com)
Iron8
It imports iron by internalizing the transferrin-iron complex through receptor-mediated endocytosis. (wikipedia.org)
The existence of a receptor for transferriniron uptake has been recognized since the late 1950s. (wikipedia.org)
The multifunctional glycolytic enzyme glyceraldehyde 3-phosphate dehydrogenase (GAPDH, EC 1.2.1.12) has been shown to utilize post translational modifications to exhibit higher order moonlighting behavior wherein it switches its function as a holo or apo transferrin receptor leading to either iron delivery or iron export respectively. (wikipedia.org)
Transferrin is the natural physiological method by which iron is transported into the cell. (genengnews.com)
Transferrin is an extracellular antioxidant, binding iron so tightly that little unbound iron is available to catalyze the production of free radicals. (genengnews.com)
These effects are thought to be at least partially independent of its iron-binding function as apo transferrin has been shown to have growth-promoting effects. (genengnews.com)
Phosphatidylinositol 3-kinase type 2α (PI3KC2α) is an essential member of the structurally unresolved class II PI3K family with crucial functions in lipid signaling, endocytosis, angiogenesis, viral replication, platelet formation and a role in mitosis. (nature.com)
The endosome is not fused with a lysosome but recycles its content back to the cell surface where soon transferrin dissociates from its receptor (Dautry-Varsat, 1986). (reactome.org)
Transferrin gold conjugates are suitable for use in cell uptake studies and can be detected using light microscopy (requires silver enhancement), darkfield microscopy, and electron microscopy. (cytodiagnostics-us.com)
The most rapid means to alter the cell surface proteome in response to stress is by alterations in endocytosis. (cell-stress.com)
Maintains1
Endocytosis in the axon initial segment maintains neuronal polarity. (uchicago.edu)
Complex1
Although much is understood of the transferrin endocytotic cycle, little has been uncovered of the molecular details underlying the formation of the receptor-transferrin complex. (rcsb.org)
Specific1
Mutation of specific Dyn2 spliced variants resulted in a differential attenuation of transferrin and dextran internalization. (elsevierpure.com)