• In line, many MLL-fusions can act as potent oncogenes in cell line models and animal models of leukemia 4 . (nature.com)
  • Our studies have demonstrated that several of the proteins encoded by cellular oncogenes function in fundamental aspects of gene regulation. (stanford.edu)
  • The normal versions of genes in the first group are called proto-oncogenes. (cancerquest.org)
  • The mutated or otherwise damaged versions of these genes are called oncogenes. (cancerquest.org)
  • We have two copies of each gene and for oncogenes, a single defective copy is enough to cause a cell to divide. (cancerquest.org)
  • Numerous genes have been identified as proto-oncogenes. (cancerquest.org)
  • As stated in the introduction to this section, the defective versions of these genes, known as oncogenes, can cause a cell to divide in an unregulated manner. (cancerquest.org)
  • In addition, these alterations affect 3 principal categories of genes, as follows: proto-oncogenes, tumor suppressor genes, and DNA repair genes. (medscape.com)
  • This article briefly discusses tumor suppressor genes and then focuses on the role of proto-oncogenes in childhood cancer. (medscape.com)
  • KH631 is a recombinant adeno-associated virus vector that encodes a human VEGF receptor fusion protein. (wboy.com)
  • Quality control test: Antibody Reactive Against Recombinant Protein. (novusbio.com)
  • NME2 (NP_002503, 51 a.a. ~ 152 a.a) partial recombinant protein with GST tag. (novusbio.com)
  • Thus, striking improvements in recombinant antigen were achieved by alternative polyadenylation signals and fusion proteins containing targeting signals designed to enhance integration or retention of HBsAg in the endoplasmic reticulum (ER) of plant cells [6]. (scielo.br)
  • This led us to construct recombinant DNA plasmids containing the encoding sequence of HBsAg and the same construction fused with the SEQDEL sequence responsible for ER sorting. (scielo.br)
  • The method involves expressing the recombinant polypeptide as a fusion protein with a pro-peptide. (justia.com)
  • The pro-peptide-polypeptide fusion protein can be cleaved and the recombinant polypeptide released under the appropriate conditions. (justia.com)
  • The preparation of valuable recombinant (genetically engineered) polypeptides, for example pharmaceutical proteins, relies frequently on techniques which involve the production of these polypeptides as fusion or hybrid proteins. (justia.com)
  • Finally, the use of a fusion partner may enable the production of a peptide which would otherwise be too small to accumulate and recover efficiently from a recombinant host cell system. (justia.com)
  • The recombinant fusion bound seven Cd ions, of which 50% were dissociated at pH 4.1. (portlandpress.com)
  • ALL cancers have lots of additional changes, the so-called 'passenger' mutations, that may contribute to the cancer, but are not the main genes. (cancerquest.org)
  • Alterations in mitochondrial dynamics due to mutations in proteins involved in the fusion-fission machinery represent an important pathogenic mechanism of human diseases. (centrodinoferrari.com)
  • An expanding number of degenerative disorders are associated with mutations in the genes encoding MFN2 and OPA1, including Charcot-Marie-Tooth disease type 2A and autosomal dominant optic atrophy. (centrodinoferrari.com)
  • Recently, mutations in the mitofusin 2 (MFN2) gene have been associated with HSMN, type 6. (neuroophthalmology.ca)
  • Despite this, we are not aware of any reports of HSMN, type VI associated with mutations in the OPA1 gene. (neuroophthalmology.ca)
  • Scope includes mutations and abnormal protein expression. (cancerindex.org)
  • Embryos homozygous for mutations in the unc-52, pat-2, pat-3, and unc- 112 genes of C. elegans exhibit a similar Pat phenotype. (illinois.edu)
  • The present study was thus undertaken to determine how TWIST1 gene mutations affect protein function. (uni-marburg.de)
  • CMT3 (also known as Dejerine-Sottas disease) is a rare congenital hypomyelinating neuropathy, which can be an autosomal dominant or a recessive disorder with mutations in several genes, including PMP22 , MPZ , and EGR2 . (msdmanuals.com)
  • At least eight mutations in the KAT6B gene have been identified in people with genitopatellar syndrome, a rare condition characterized by genital abnormalities, missing or underdeveloped kneecaps (patellae), intellectual disability, and abnormalities affecting other parts of the body. (medlineplus.gov)
  • The mutations that cause genitopatellar syndrome occur near the end of the KAT6B gene in a region known as exon 18. (medlineplus.gov)
  • More than 10 mutations in the KAT6B gene have been found to cause the Say-Barber-Biesecker-Young-Simpson (SBBYS) variant of Ohdo syndrome. (medlineplus.gov)
  • Mutations that cause the SBBYS variant of Ohdo syndrome have been identified throughout the KAT6B gene, although many of them occur in exon 18. (medlineplus.gov)
  • Studies suggest that these mutations likely prevent the production of functional histone acetyltransferase from one copy of the KAT6B gene in each cell. (medlineplus.gov)
  • [ 2 , 3 , 4 ] A full list of the described mutations is available at the TBX5 Gene Mutation Database , an online locus-specific database that contains germline and somatic mutations of the TBX5 gene. (medscape.com)
  • Mutations of this gene introduce a premature stop codon and result in truncated protein versions. (medscape.com)
  • MLL-AF4 promotes leukemogenesis by activating key target genes, mainly through recruitment of DOT1L and increased histone H3 lysine-79 methylation (H3K79me2/3). (iric.ca)
  • In addition to its role in H3K36 tri-methylation, SETD2 is required to maintain high H3K79 di-methylation and MLL-AF9-binding to critical target genes, such as Hoxa9 . (nature.com)
  • As the SEC can regulate the transcriptional activity of RNA polymerase II, it was hypothesized that these MLL-fusions induce aberrant regulation of transcriptional elongation on MLL-target genes 14 . (nature.com)
  • These are frequently activated by fusion to other transcriptional proteins resulting in chimeric transcription factors. (stanford.edu)
  • We are studying the effects and consequences of protein fusion on the transcriptional and transforming activities of these proteins using in vitro and animal models. (stanford.edu)
  • We demonstrate here that GSK-3 maintains the MLL leukemia stem cell transcriptional program by promoting the conditional association of CREB and its coactivators TORC and CBP with homedomain protein MEIS1, a critical component of the MLL-subordinate program, which in turn facilitates HOX-mediated transcription and transformation. (stanford.edu)
  • Transcriptional targeting in the airway using novel gene regulatory elements. (nih.gov)
  • The resulting fusion protein has the DNA binding activity of Fli-1 and, with the EWS domain, it is also a more potent transcriptional activator than Fli-1 itself. (bdbiosciences.com)
  • Bailly RA, Bosselut R, Zucman J. DNA-binding and transcriptional activation properties of the EWS-FLI-1 fusion protein resulting from the t(11;22) translocation in Ewing sarcoma. (bdbiosciences.com)
  • The Ewing's sarcoma EWS/FLI-1 fusion gene encodes a more potent transcriptional activator and is a more powerful transforming gene than FLI-1. (bdbiosciences.com)
  • I wanted to determine if the TWIST protein or its conserved motifs interact with other regulatory proteins to help to regulate the TWIST transcriptional activity. (uni-marburg.de)
  • Furthermore, two more highly conserved TWIST motifs NSEEE and WR were analyzed individually to find out their interacting proteins and their role in regulating the TWIST1 transcriptional activity. (uni-marburg.de)
  • The GlmS(6Ala) protein and the CBD-tagged proteins are functional in vivo according to assays conducted at New England Biolabs. (neb.com)
  • These results indicate that the p10 protein is produced from the viral genome and that the protein is functional. (virology.ws)
  • Functional investigation of 128 conserved MLL-fusion-interactors identifies a specific role for the lysine methyltransferase SETD2 in MLL-leukemia. (nature.com)
  • Families of related leukemia fusion proteins that share genomic and biological properties represent unique opportunities to study how the combination of distinct functional protein modules can drive oncogenic transformation. (nature.com)
  • It has therefore been proposed that the oncogenic activity of MLL-fusion proteins depends on chromatin targeting functions exerted by the MLL N-terminus in combination with other functional properties encoded by the fusion partners 6 . (nature.com)
  • Chemoplexy results in the formation of functional EWSR1-FLI1 or ESWR1-ERG fusions that provide a selective growth or survival advantage. (standardofcare.com)
  • Interestingly, MFN2 shows functional overlap with the OPA1 gene, the protein underlying the most common form of autosomal dominant optic atrophy. (neuroophthalmology.ca)
  • The aim of my work is to characterize functional domains in TWIST protein, and to determine the interacting partner for TWIST and its motifs particularly NSEEE and WR. (uni-marburg.de)
  • These changes include elongation, polarization, aggregation and fusion, which ultimately give rise to functional muscle. (biologists.com)
  • [ 1 ] His prediction was subsequently supported by the cloning of the retinoblastoma tumor suppressor gene ( RB1 ) and by functional studies of the retinoblastoma protein, Rb. (medscape.com)
  • Spiller B. O., Blackbourne, D., Mark L, Proctor D. and * Blom A. M. , (2003) Functional activity of the complement regulator encoded by Kaposi's sarcoma associated herpesvirus. (lu.se)
  • The most relevant proteins involved in the mitochondrial fusion process are three GTPase dynamin-like proteins: mitofusin 1 (MFN1) and 2 (MFN2), located in the outer mitochondrial membrane, and optic atrophy protein 1 (OPA1), in the inner membrane. (centrodinoferrari.com)
  • This review provides an overview of the basic molecular mechanisms involved in mitochondrial fusion and focuses on the alteration in mitochondrial DNA amount resulting from impairment of mitochondrial dynamics. (centrodinoferrari.com)
  • We also review the literature describing the main disorders associated with the disruption of mitochondrial fusion. (centrodinoferrari.com)
  • Mitochondrial fusion and fission are fundamental processes underlying cellular dynamics [1]. (centrodinoferrari.com)
  • CMT2A is the most common CMT2 phenotype, most often due to a mutation in the gene that encodes mitochondrial fusion protein mitofusin-2 ( MFN2 ). (msdmanuals.com)
  • In terms of gene expression, the viral RNA-dependent RNA polymerase binds the encapsulated genome at the leader region, which starts the transcription process. (wikipedia.org)
  • The mutated GlmS protein does not bind Ni-NTA resin in the presence of 20mM imidazole binding/ wash buffer, whereas wt GlmS protein binds Ni-NTA resin and is not eluted until the imidazole concentration is within 55-80 mM (1). (neb.com)
  • Like other ets-related proteins it binds to consensus sites in DNA through a 85 amino acid ets domain in the carboxyl region of the protein. (bdbiosciences.com)
  • Thus this paper describes, for the first time, an MT gene identified from macroalgae which is induced by copper exposure and whose encoded protein product binds cadmium and copper. (portlandpress.com)
  • Trouw L., Nilsson S., Goncalvez I., Landberg G. and Blom A. M. (2005) C4b-binding protein binds to necrotic cells and DNA, which limits DNA release and inhibits complement activation. (lu.se)
  • Hartmann M, Schneider TR, Pfeil A, Heinrich G, Lipscomb WN, Braus GH (2003) Evolution of feedback-inhibited beta /alpha barrel isoenzymes by gene duplication and a single mutation. (springer.com)
  • A missense mutation in this gene is associated with Van der Woude syndrome 2. (nih.gov)
  • Mutation of CFAP57, a protein required for the asymmetric targeting of a subset of inner dynein arms in Chlamydomonas, causes primary ciliary dyskinesia. (nih.gov)
  • Germline mutation of one TP53 allele is found in patients with Li-Fraumeni syndrome who generally inherit a mutated TP53 gene from an affected parent. (medscape.com)
  • and a large polymerase subunit, encoded by L. In addition, the unedited P gene contains the V protein. (wikipedia.org)
  • In situ hybridization analysis of AMPA receptor subunit gene expression in the developing rat spinal cord. (bcm.edu)
  • Kask L., Hillarp A., Ramesh B., Dahlbäck B., and Blom A. M. (2002) Structural requirements for the intra-cellular subunit polymerization of the complement inhibitor C4b-binding protein. (lu.se)
  • GFP fusion protein in the cytoplasm (middle panel). (invivogen.com)
  • The protein encoded by this gene is prenylated at its C-terminus, and localizes to the cytoplasm and plasma membrane. (cancerindex.org)
  • The CHS protein is expressed in the cytoplasm of cells of a variety of tissues and may represent an abnormality of organellar protein trafficking. (medscape.com)
  • Secondly, those proteins which are present in soluble form in the cytoplasm often are subject to degradation by host specific enzymes, thus reducing the amounts of active protein that can be recovered. (justia.com)
  • The classical Complex I enzyme is composed of seven membrane-spanning protein subunits and seven protein subunits protruding into the cytoplasm (in bacteria) or into the mitochondrial matrix (in eukaryotes). (lu.se)
  • If the membrane-anchor is missing or defective, Fp and Ip subunits accumulate as soluble proteins in the cytoplasm. (lu.se)
  • This is in contrast with tumor suppressor genes which must BOTH be defective to lead to abnormal cell division. (cancerquest.org)
  • Inactivation of tumor suppressor genes, whose products normally provide negative control of cell proliferation, contributes to malignant transformation in various cell types. (medscape.com)
  • Like Rb protein, many of the proteins encoded by tumor suppressor genes act at specific points in the cell cycle. (medscape.com)
  • Another important class of tumor suppressor genes involved in cell cycle control and in the generation of human cancers is the cyclin-dependent kinase (CDK) inhibitors. (medscape.com)
  • TtCM was purified to homogeneity on an SDS polyacrylamide gel as a His-fusion protein with a deduced molecular mass of 15.8 kDa. (springer.com)
  • The molecular sequence of this clone aligns with the gene accession number as a point of reference only. (origene.com)
  • Our most recent work focuses on the molecular mechanisms of the aberrant gene silencing in the malignant lymphoid cells. (upenn.edu)
  • MLL-fusions represent a large group of leukemia drivers, whose diversity originates from the vast molecular heterogeneity of C-terminal fusion partners of MLL. (nature.com)
  • While studies of selected MLL-fusions have revealed critical molecular pathways, unifying mechanisms across all MLL-fusions remain poorly understood. (nature.com)
  • Numerous studies have established strong links between the molecular function of the fusion partner and the mechanistic basis of oncogenic transformation in MLL-fusion-induced leukemogenesis 4 . (nature.com)
  • However, it is unclear whether these molecular mechanisms pertain to the entire family of MLL-fusions or if they specifically affect the leukemogenicity of isolated MLL-fusion proteins. (nature.com)
  • This mechanism also applies to hematopoietic cells transformed by other HOX genes, including CDX2, which is highly expressed in a majority of acute myeloid leukemias, thus providing a molecular approach based on GSK-3 inhibitory strategies to target HOX-associated transcription in a broad spectrum of leukemias. (stanford.edu)
  • Aberrant chimeric RNA GOLM1-MAK10 encoding a secreted fusion protein as a molecular signature for human esophageal squamous cell carcinoma. (bcm.edu)
  • This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. (cancerindex.org)
  • An S-peptide Fucus MT fusion protein expressed in Escherichia coli exhibited a relative molecular mass of ∼ 14 kDa. (portlandpress.com)
  • Therefore, in the lab we working with molecular biological approaches such as making mutants and fusion proteins but also purely biochemical/biophysical projects, purifying proteins for reconstitution into liposomes, where the ion translocation activities can be measured by Na+ NMR using a shift reagent or by a pH sensitive, membrane-impermeable dye denoted Glu3. (lu.se)
  • Mark L., Lee W. H., Villoutreix B. O., Proctor D., Blackbourn, D., Spiller B. O. and Blom A. M. (2004) KSHV complement control protein mimics human molecular mechanisms for inhibition of the complement system. (lu.se)
  • Antimicrobial susceptibility testing, molecular typing, characterization of ESBL-encoding genes and the genetic environment, conjugation experiments and plasmid analysis were carried out. (who.int)
  • A novel coronavirus isolated from bats in China is unusual because the genome contains a gene from a virus in a completely different family, the Reoviridae (link to paper) . (virology.ws)
  • This sequence, called p10, was also detected in viral mRNAs from infected bats, further demonstrating the presence of the reovirus-like gene in the coronavirus genome. (virology.ws)
  • Then, we selected 48 genes that may encode uncharacterised effectors (e. g. no detectable amino acid identity to other proteins or with a particular putative biochemical activity) from the genome of C. trachomatis strain L2/434/Bu. (europa.eu)
  • Moreover, genome instability was attributed to impaired autophagy and several autophagy genes with tumor suppressor functions (e.g. (hindawi.com)
  • Of the 30,000 or so genes that are currently thought to exist in the human genome , there is a small subset that seems to be particularly important in the prevention, development, and progression of cancer. (cancerquest.org)
  • CpGV encodes six genes required for genome replication, which includes DNA polymerase and helicase. (kenyon.edu)
  • Despite the differences in their normal roles, these genes all contribute to unregulated cell division if they are present in a mutant (oncogenic) form. (cancerquest.org)
  • Aberrant cell signaling and epigenetic regulation of gene expression in human lymphomas. (upenn.edu)
  • We are studying the role that normal chromatin structure plays in gene regulation in hematopoietic cells and how its disruption leads to altered development and cancer. (stanford.edu)
  • This basic two-step process has been confirmed in most mammalian cells, although its regulation and the repertoire of specific accessory proteins are likely to be highly context dependent. (centrodinoferrari.com)
  • MFN2 encodes an outer mitochondrial membrane protein that is important in the regulation of fusion of the mitochondria. (neuroophthalmology.ca)
  • TP53 activates the expression of genes involved in apoptosis, cell cycle regulation (p21), and MDM2. (medscape.com)
  • Researchers suspect that the shortened enzyme may function differently than the full-length version, altering the regulation of various genes during early development. (medlineplus.gov)
  • A shortage of this enzyme impairs the regulation of various genes during early development. (medlineplus.gov)
  • These changes likely alter histone modification, which could prevent normal regulation of gene activity. (medlineplus.gov)
  • Impaired gene regulation may contribute to the growth of cancers by allowing abnormal cells to grow and divide uncontrollably. (medlineplus.gov)
  • Sjöberg A., Trouw L., McGrath F., Hack E. C. and Blom A. M. (2006) Regulation of complement activation by C-reactive protein: targeting of the inhibitory activity of C4b-binding protein. (lu.se)
  • For example, the TP53 gene, located on chromosome 17, encodes a 53-kd nuclear protein that functions as a cell cycle checkpoint. (medscape.com)
  • The genetic change associated with uterine leiomyomas is a translocation between the region of chromosome 10 containing the KAT6B gene and a particular region of chromosome 17. (medlineplus.gov)
  • Expression of ALK in malignant T cells is typically due to the t(2;5) translocation resulting in formation of the fusion gene which encodes a 80-kDa hybrid protein that contains portion of the nuclear protein nucleophosmin (NPM) joined to the entire cytoplasmic portion of the receptor tyrosine kinase ALK. (upenn.edu)
  • Occlusion bodies (Occluded virus, or OV) of granuloviruses contain one or two virions that are wrapped in a protein called granulin (a protein that distinguishes granuloviruses from nuclear polyhedrosis viruses) (Fields Virology, 2013). (kenyon.edu)
  • This synergistic effect is consistent with the observation that combined K38R (NLS1) and K76R (NLS2) mutants dramatically reduced nuclear localization, further suggesting that both NLS1 and NLS2 work together in regulating nuclear localization of TWIST protein. (uni-marburg.de)
  • Critical for cellular organization, phosphatidylinositol phosphate (PIP) lipids regulate the localization and activity of numerous proteins across intracellular membranes in eukaryotic cells ( Di Paolo and De Camilli 2006 ). (elifesciences.org)
  • 1) Characterize novel families of oncoproteins (Pbx and Meis) that dimerize with and regulate the DNA binding properties of Hox proteins. (stanford.edu)
  • The mutant proteins often retain some of their capabilities but are no longer sensitive to the controls that regulate the normal form of the protein. (cancerquest.org)
  • Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. (cancerindex.org)
  • It appears to regulate genes that are important for early development, including development of the skeleton and nervous system. (medlineplus.gov)
  • Fusion of the outer mitochondrial membrane depends on two GTPase family members: mitofusin 1 (MFN1) and mitofusin 2 (MFN2). (centrodinoferrari.com)
  • The largest family of "multi-partner translocations" in acute leukemia comprises fusions involving the product of the KMT2A ( MLL) gene. (nature.com)
  • 120 different translocations, resulting in the generation of MLL-fusion proteins encompassing more than 75 different partner genes 5 . (nature.com)
  • Chemoplexy, rather than simple reciprocal translocations define the gene fusions in about 42% of patients. (standardofcare.com)
  • The genetic changes are chromosomal rearrangements (translocations) that disrupt the region of chromosome 10 containing the KAT6B gene. (medlineplus.gov)
  • It is unclear how translocations involving the KAT6B gene are related to the development of cancer. (medlineplus.gov)
  • The order from left to right is the leader region, followed by the nucleocapsid, then the phosphoprotein, then the matrix protein, followed by the fusion protein, then the large polymerase, and finally the trailer sequence. (wikipedia.org)
  • T7 RNA Polymerase: T7 gene1 is encoded by the lambda DE3 prophage present within the chromosome. (neb.com)
  • In addition to the HA and NA, the RNA transcription and replication complex (PB2, PB1, PA, NP) also have species-speci c determinants that impact e cient replication in humans and other mammals, particularly polymerase basic protein 2 (PB2). (cdc.gov)
  • The unc-52 (perlecan), pat-2 α-integrin), and pat-3 (β-integrin) genes encode ECM or transmembrane proteins found at the cell - matrix adhesion sites of both dense bodies and M-lines. (illinois.edu)
  • Combet C, Blanchet C, Geourjon C, Deleage G (2000) NPS@: network protein sequence analysis. (springer.com)
  • Faraldo MM, de Pedro MA, Berenguer J (1992) Sequence of the S-layer gene of Thermus thermophilus HB8 and functionality of its promoter in Escherichia coli . (springer.com)
  • Read-through transcription from the neighboring upstream gene (NME1) generates naturally-occurring transcripts (NME1-NME2) that encode a fusion protein comprised of sequence sharing identity with each individual gene product. (origene.com)
  • They actually made discoveries of several unique DNA sequences which were published in the gene sequence database of the National Center for Biotechnology Information at the National Library of Medicine. (twu.edu)
  • They went from a gene identity to moving that gene into a plasmid, sequencing it, finding out the sequence, and finding out the encoded protein's localization in a semester's work. (twu.edu)
  • It is caused by nucleotide sequence changes within or in proximity of the TWIST1 gene. (uni-marburg.de)
  • Although the protein sequence showed high identity with plant and invertebrate MTs, it contained a unique 'linker ' region (14 amino acid residues) between the two putative metal-binding domains which contained no cysteine residues. (portlandpress.com)
  • Although λ DE3 is normally dormant in the host chromosome, the induction of the SOS response can occur as the result of expressing proteins that damage the E. coli chromosome, either directly or indirectly. (neb.com)
  • Therefore, these results demonstrate that LsARF3 could promote lettuce bolting in response to the high temperature by directly or indirectly activating the expression of floral genes such as LsCO, which provides new insights into lettuce bolting in the context of ARFs signaling and heat response. (frontiersin.org)
  • Genes whose protein products can directly or indirectly prevent cell division or lead to cell death. (cancerquest.org)
  • Because cancer-driven by a single oncogene, such as leukemia driven by the BCR-ABL fusion gene, is amenable to treatment with inhibitors that target that very single causative agent, there is a lot of interest to develop therapies to treat the EHE. (oist.jp)
  • Ph+ acute lymphoblastic leukemia (Ph+ ALL) is a high-risk acute leukemia with poor prognosis, in which the specific t(9;22)(q34;q11) translocation results in a chimeric bcr-abl (e1a2 breakpoint) and in a 190 KD protein (p190) with constitutive tyrosine kinase activity. (hindawi.com)
  • This translocation brings the 5' region of the EWS gene into conjunction with the 3' region of the Fli-1 gene encoding the ets-DNA binding domain. (bdbiosciences.com)
  • Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by NME1) and 'B' (encoded by this gene) isoforms. (novusbio.com)
  • The class 1A phosphoinositide 3-kinase (PI3K) beta (PI3Kβ) is functionally unique in the ability to integrate signals derived from receptor tyrosine kinases (RTKs), heterotrimeric guanine nucleotide-binding protein (G-protein)-coupled receptors (GPCRs), and Rho-family GTPases. (elifesciences.org)
  • Previous experiments have not been able to elucidate whether interactions with membrane-tethered proteins primarily control PI3Kβ localization versus directly modulate lipid kinase activity. (elifesciences.org)
  • q11) resulting in a chimeric bcr-abl (e1a2 breakpoint) fusion gene that encodes a 190 KD protein (p190) with constitutively active tyrosine kinase activity that can alter multiple signaling pathways, contributing to tumor growth and proliferation. (hindawi.com)
  • A recurrent BCAM-AKT2 fusion gene leads to a constitutively activated AKT2 fusion kinase in high-grade serous ovarian carcinoma. (bcm.edu)
  • Anaplastic lymphoma kinase (ALK) protein may be detected in most cases (60-70%) of systemic ALCL by immunohistochemistry. (medscape.com)
  • InvivoGen also provides THP1-KO-ASC cells featuring a biallelic knockout of the human ASC gene. (invivogen.com)
  • These cells do not express the ASC protein and display a complete abrogation of mature IL-1β secretion upon activation of the canonical and non-canonical inflammasomes. (invivogen.com)
  • For detecting and quantifying the release of mature human (h)IL-1β, InvivoGen provides HEK-Blue™ IL-1β sensor cells , which express an NF-κB-inducible SEAP reporter gene. (invivogen.com)
  • Cells deficient in both these proteases are much more amenable to the production of proteins from cloned genes. (neb.com)
  • When the p10 gene from the bat coronavirus was expressed in cells, formation of syncytia (fused cells) was observed. (virology.ws)
  • We have raised rabbit polyclonal antibodies against 5 of these proteins and we are currently analysing their subcellular localisation in C. trachomatis infected cells. (europa.eu)
  • These ATG genes are highly conserved in mammalian cells, allowing to study their functions also in higher eukaryotes [ 1 - 4 ]. (hindawi.com)
  • Expression of MLL-fusions enhances proliferation and blocks myeloid differentiation of hematopoietic progenitor cells, leading to their pathological accumulation. (nature.com)
  • Genes whose protein products stimulate or enhance the division and viability of cells. (cancerquest.org)
  • Occlusion bodies are crystalline gene products that are responsible for the primary viral infection in the gut basal cells. (kenyon.edu)
  • The CHS gene affects the synthesis and/or maintenance of storage/secretory granules in various types of cells. (medscape.com)
  • Accordingly, the subcellular localization of NLS1 and NLS2 in TWIST protein was investigated in U2-OS cells following co-transfection with E12. (uni-marburg.de)
  • The direct interaction of SEF2 with TWIST was verified in a yeast mating assay and then confirmed in an in vivo NLS-rescue assay using U2-OS cells, showing that SEF2 forms a heterodimer with TWIST protein and co-localized into the nucleus. (uni-marburg.de)
  • Production of the fusion polypeptide involves the introduction of the hybrid gene into a biological host cell system, for example yeast cells, which permits the expression and accumulation of the fusion polypeptide. (justia.com)
  • This methodology facilitates the isolation and recovery of the fusion peptide from the host cells by exploiting the physico-chemical properties of the fusion partner. (justia.com)
  • The TP53 gene is also capable of stimulating apoptosis of cells containing damaged DNA. (medscape.com)
  • The hemagglutinin (HA) gene codes for one of the two surface glycoproteins and is central to species specificity because it is responsible for virus attachment and fusion with host cells. (cdc.gov)
  • It is active in cells and tissues throughout the body, where it interacts with many other proteins. (medlineplus.gov)
  • mRNAs are capped and polyadenylated by the L protein, and the V protein is created through editing the P mRNA gene, as mentioned earlier. (wikipedia.org)
  • The second screening of the yeast clones suggested some promising candidates protein as interacting partners with the NSEEE motif such as ETV5, SURF4, Spastin, Metalloproteinase 2, and ALR-like protein mRNA. (uni-marburg.de)
  • Somatic changes involving the KAT6B gene have also been identified in some people with uterine leiomyomas, which are noncancerous growths in the uterus that are also known as uterine fibroids. (medlineplus.gov)
  • The outside of the nucleocapsid contains proteins that form ring-shaped subunits through their interactions. (kenyon.edu)
  • These events are dependent on the proper levels of transcription and translation of certain genes. (cancerquest.org)
  • By adding a small molecule called an acetyl group to particular locations on histones, histone acetyltransferases control the activity of certain genes. (medlineplus.gov)
  • Multiple alternatively spliced transcript variants have been found for this gene. (origene.com)
  • Multiple alternatively spliced variants, encoding the same protein, have been identified. (cancerindex.org)
  • Studies in yeast have identified a series of autophagy- (ATG-) related genes forming the autophagy machinery. (hindawi.com)
  • The second aim of my work was to identify the interacting proteins that could influence the functionality of TWIST using yeast-two-hybrid assay. (uni-marburg.de)
  • Orthologous to human NAPA (NSF attachment protein alpha). (nih.gov)
  • Humans have a duplicate set of 23 chromosomes (or a total of 46 chromosomes) in any given cell that carries all of the human genes. (sarcomahelp.org)
  • HER2/neu (also called ERB B2 ) is the gene that encodes the human epidermal growth factor receptor type 2. (cancerquest.org)
  • WD-repeat proteins: structure characteristics, biological function, and their involvement in human diseases. (nih.gov)
  • Find NAPG Proteins for a variety of species such as anti-Human NAPG, anti-Arabidopsis thaliana NAPG, anti-Mouse NAPG. (antibodies-online.com)
  • The 720-amino acid UNC-112 protein is homologous to Mig-2, a human protein of unknown function. (illinois.edu)
  • No. 4,743,679 which discloses a process for the production of human epidermal growth factor comprising cleavage of a fusion protein by Staphylococcus aureus V8 protease. (justia.com)
  • Although carcinogenic roles for the INK4B, INK4C, INK4D, CIP1, KIP1, and KIP2 genes appear to be limited, INK4A is among the most commonly mutated genes in human tumors. (medscape.com)
  • The responsible gene has been mapped to band 12q24.1, which encodes the human transcription factor TBX5. (medscape.com)
  • The T-box gene family is a group of related genes that play a critical role in human embryonic development. (medscape.com)
  • Blom A. M . , Berggård K., Webb J. H., Villoutreix B., Lindahl G. and Dahlbäck B. (2000) Human C4b-binding protein has overlapping but not identical binding sites for C4b and streptococcal M-proteins. (lu.se)
  • However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. (origene.com)
  • protein sequences were analysed with a major concern [1,2]. (who.int)
  • 25 of the genes are unique to CpGV, while 118 are homologous to other ''Baculovirus'' species. (kenyon.edu)
  • Autophagosomes engulf cytoplasmatic components and deliver these cargos, for example, long-lived proteins or damaged mitochondria, to lysosomes for degradation. (hindawi.com)
  • Mitochondria are highly dynamic, complex organelles that continuously alter their shape, ranging between two opposite processes, fission and fusion, in response to several stimuli and the metabolic demands of the cell. (centrodinoferrari.com)
  • Fusion allows the exchange of contents, DNA, and metabolites between neighboring mitochondria, including damaged or senescent mitochondria, promoting their survival [2, 3]. (centrodinoferrari.com)
  • Chédiak-Higashi syndrome (CHS) is an autosomal recessive immunodeficiency disorder characterized by abnormal intracellular protein transport. (medscape.com)
  • Saethre-Chotzen syndrome is an autosomal dominant inherited disorder with premature fusion of cranial sutures. (uni-marburg.de)
  • 2) We have discovered a group of oncoproteins that are implicated in long-term maintenance of gene expression through their effects on the state of chromatin. (stanford.edu)
  • To decipher how amplification of PI(3,4,5)P 3 arises from the relay of signals between cell surface receptors, lipids, and peripheral membrane proteins, we must understand how membrane localization and activity of PI3Ks is regulated by different signaling inputs. (elifesciences.org)
  • Exogenous control of mammalian gene expression via modulation of RNA self-cleavage. (bcm.edu)
  • Analysis of this HA gene shows that it is closely related to avian A(H5) viruses in HA clade 2.3.4.4b and lacked amino acid changes that improve recognition of mammalian receptors or fusion of the viral membrane with the host endosomal membranes. (cdc.gov)
  • A(H5) viruses in HA clade 2.3.4.4b and lacked amino acid changes that improve recognition of mammalian receptors or fusion of the viral membrane with the host endosomal membranes. (cdc.gov)
  • Na(+) influx via Orai1 inhibits intracellular ATP-induced mTORC2 signaling to disrupt CD4 T cell gene expression and differentiation. (nih.gov)
  • This gene encodes for a bHLH transcription factor, which inhibits osteogenic differentiation by transc. (uni-marburg.de)
  • Major steps in the autophagic process include initiation, nucleation, elongation, and maturation of the autophagosomes as well as fusion of the autophagosomes to lysosomes. (hindawi.com)
  • Interestingly, recent data suggest a function for ULK1 not only during autophagy activation but also during elongation and closure of the autophagosomal membrane via binding to ATG8 proteins [ 8 ]. (hindawi.com)
  • Floral inducers including FLOWERING LOCUS T (FT), FD, SUPPRESSOR OF CONSTANS 1 (SOC1), and LFEAY (LFY) activate the transcription of the majority of genes floral genes, promoting Arabidopsis bolting and flowering ( Johansson and Staiger, 2015 ). (frontiersin.org)
  • The p19ARF protein, which is encoded by the same locus as p16, also leads to cell cycle arrest by inhibiting the ability of MDM2 to inactivate TP53. (medscape.com)
  • This locus, however, also encodes a protein from an alternative reading frame, designated p19ARF. (medscape.com)
  • A vesicle trafficking protein αSNAP regulates Paneth cell differentiation in vivo. (nih.gov)
  • E. coli B strains are "naturally" deficient in the Lon protease which in K-12 strains serves to degrade misfolded proteins and to prevent some cell cycle-specific proteins from accumulating. (neb.com)
  • Deletion of this gene confers resistance to this type of phage, but does not significantly affect the transformation or growth characteristics of the cell. (neb.com)
  • In bat reoviruses, the p10 gene is known to encode a protein that causes cell fusion. (virology.ws)
  • Chromosomes are the cellular storage units for genes contained within the nucleus (which is the genetic center) of the cell and are analogous to a spool with the DNA or genetic message being the thread on the spool. (sarcomahelp.org)
  • Antibody reactivity against recominant protein and cell lysate for WB. (novusbio.com)
  • 1. Role of the cytokine-signal transduction pathways and epigenetic gene silencing in pathogenesis of T-cell lymphoma. (upenn.edu)
  • The genes that have been identified to date have been categorized into two broad categories, depending on their normal functions in the cell. (cancerquest.org)
  • This first category also includes genes that contribute to tumor growth by inhibiting cell death. (cancerquest.org)
  • Many of these genes are responsible for providing the positive signals that lead to cell division. (cancerquest.org)
  • Overexpression of this gene is associated with tumor cell proliferation and metastasis. (cancerindex.org)
  • DRAM2 overexpression also increased proteins CDK4, CyclinD3, and decreased p27 expression, all of which are cell cycle-related factors. (cancerindex.org)
  • Auxiliary genes include proteases, such as chitinase, which are dedicated to the degradation of host structures and the prevention of host cell death (Luque et al, 2001). (kenyon.edu)
  • The CDK4-cyclinD complex normally phosphorylates the retinoblastoma protein (Rb protein), leading to release of the E2F transcription factor and cell cycle progression. (medscape.com)
  • The p16INK4A protein is a cell-cycle inhibitor that acts by inhibiting activated cyclin D:CDK4/6 complexes, which play a crucial role in the control of the cell cycle by phosphorylating Rb protein. (medscape.com)
  • Inoculation with a high dose strains of LMP1 transgenic mice vide a powerful tool in mechanistic of EBV caused a B-cell lymphopro- were established that express LMP1 studies on the role of individual viral liferative disorder in these mice, under the control of the immunoglob- genes in cancer. (who.int)
  • Chengdu Origen is a clinical-stage gene therapy company focused on developing gene therapy for unmet medical needs and providing meaningful clinical benefits for patients suffering from genetic and chronic diseases and has comprehensive viral vector manufacturing capabilities and infrastructure. (wboy.com)
  • These techniques are based upon the preparation of hybrid genes, i. e. genes comprising genetic material encoding the polypeptide of interest linked to genetic material additional to the gene of interest. (justia.com)
  • Genetic changes involving the KAT6B gene have been associated with certain types of cancer. (medlineplus.gov)
  • A cardiomelic developmental field has also been postulated to relate the genetic heterogeneity of HOS (and other similar syndromes) to a cascade of molecules, including the brachyury, sonic hedgehog, bone morphogenetic protein, retinoic acid receptor, and transforming growth factor beta families. (medscape.com)
  • Evolutionary alignment of Twist proteins from different species, indicate TWIST contain 4 additional conserved regions such as NSEEE, NLS1, NLS2, and WR-domain besides the bHLH motif. (uni-marburg.de)
  • BMV (as well as a parainfluenza virus known as PIV5) contain short hydrophobic proteins, which have a role in blocking the TNFalpha-mediated apoptosis pathway. (wikipedia.org)
  • Webb, J. H., Villoutreix, B. O., Dahlbäck, B. and Blom A. M. (2001) Localization of a hydrophobic binding site for protein S on the b-chain of C4BP. (lu.se)
  • The Fas proteins synthesize, modify, and activate a mixture of growth regulating cytokinins, which cause a hormonal imbalance in plants, resulting in abnormal growth. (usda.gov)
  • Complex karyotypes are the product of chemoplexy (a sudden burst of complex, loop like gene arrangements that give rise to a fusion gene), and these fusions arise early, giving rise to both primary and relapsed Ewing sarcoma tumors, which can continue to evolve in parallel. (standardofcare.com)
  • Alternative splicing of this gene results in multiple transcript variants. (nih.gov)
  • Chen X, Antson AA, Yang M, Li P, Baumann C, Dodson EJ, Dodson GG, Gollnik P (1999) Regulatory features of the trp operon and the crystal structure of the trp RNA-binding attenuation protein from Bacillus stearothermophilus . (springer.com)
  • METHODS: CDX2 and mucin protein expressions were examined by immunohistochemistry and compared with survival and adjuvant chemotherapy benefits in a prospective evaluation cohort of 782 stage II/III GC patients. (bvsalud.org)
  • BACKGROUND/AIM: Colorectal cancer (CRC) with reduced expression of the homeobox transcription factor CDX2, a master gene essential for the development and maintenance of the intestinal tract, is known as a poor prognosis subtype of CRC. (bvsalud.org)