• Successful reprogramming of epiblast stem cells by blocking nuclear localization of beta-catenin. (nature.com)
  • ChemDiv's Inhibitors of beta-Catenin Signaling Library contains 8,000 compounds. (chemdiv.com)
  • ChemDiv proposes the new library of beta-catenin inhibitors/modulators. (chemdiv.com)
  • The primary structure of beta-catenin consists of an NH2-terminal domain (NDT), a central region represented by 12 Arm-repeats (R1-12), and a COOH-terminal end (CTD). (chemdiv.com)
  • Scholars@Duke publication: Protein phosphatase 1 regulates assembly and function of the beta-catenin degradation complex. (duke.edu)
  • The Wnt/beta-catenin signaling pathway is critical in both cellular proliferation and organismal development. (duke.edu)
  • However, how the beta-catenin degradation complex is inhibited upon Wnt activation remains unclear. (duke.edu)
  • Using a directed RNAi screen we find that protein phosphatase 1 (PP1), a ubiquitous serine/threonine phosphatase, is a novel potent positive physiologic regulator of the Wnt/beta-catenin signaling pathway. (duke.edu)
  • PP1 expression synergistically activates, and inhibition of PP1 inhibits, Wnt/beta-catenin signaling in Drosophila and mammalian cells as well as in Xenopus embryos. (duke.edu)
  • Inhibition of PP1 leads to enhanced phosphorylation of specific sites on axin by casein kinase I. Axin phosphorylation markedly enhances the binding of glycogen synthase kinase 3, leading to a more active beta-catenin destruction complex. (duke.edu)
  • Wnt-regulated changes in axin phosphorylation, mediated by PP1, may therefore determine beta-catenin transcriptional activity. (duke.edu)
  • Specific inhibition of PP1 in this pathway may offer therapeutic approaches to disorders with increased beta-catenin signaling. (duke.edu)
  • Members from the plakophilin-catenin sub-family (Pkp-1 -2 and -3) facilitate the linkage of desmosome junctional parts to one Col6a3 another (e. jobs of related catenins such as for example beta-catenin and p120-catenin structurally. (healthyconnectionsinc.com)
  • Intro Plakophilins (Pkps) type a subfamily of catenins each including an Armadillo-repeat site that's structurally homologous to the people present in people from the beta-catenin and p120-catenin subfamilies [1]-[6]. (healthyconnectionsinc.com)
  • Conceivably some Pkp-catenin jobs might later end up being analogous to the people from the beta- and p120-catenin subfamily people that associate straight with transcription-factors (e.g. beta-catenin binds/modulates TCF/LEF and p120-catenin binds/modulates Kaiso) [32]-[34]. (healthyconnectionsinc.com)
  • Tandem repeats form superhelix of helices that is proposed to mediate interaction of beta-catenin with its ligands. (embl.de)
  • The cylindrical structure features a positively charged grove, which presumably interacts with the acidic surfaces of the known interaction partners of beta-catenin. (embl.de)
  • It is now clear that Armadillo and beta-catenin bind directly to members of the T-cell factor/lymphoid enhancer factor subfamily of HMG box DNA-binding proteins, forming bipartite transcription factors that regulate Wingless/Wnt responsive genes in both Drosophila and vertebrates. (embl.de)
  • Three-dimensional structure of the armadillo repeat region of beta-catenin. (embl.de)
  • Beta-catenin is essential for cadherin-based cell adhesion and Wnt/Wingless growth factor signaling. (embl.de)
  • A core region of beta-catenin, composed of 12 copies of a 42 amino acid sequence motif known as an armadillo repeat, mediates these interactions. (embl.de)
  • The three-dimensional structure of a protease-resistant fragment of beta-catenin containing the armadillo repeat region has been determined. (embl.de)
  • Although unrelated in sequence, the beta-catenin binding regions of cadherins, Tcfs, and APC are acidic and are proposed to interact with this groove. (embl.de)
  • Signal transduction of beta-catenin. (embl.de)
  • Beta-catenin participates in signal transduction and developmental patterning in Xenopus and Drosophila embryos as a component of the Wnt signaling pathway. (embl.de)
  • The adenomatous polyposis coli tumor suppressor protein is also implicated in beta-catenin signaling. (embl.de)
  • In attempting to define the mechanism underlying this inhibition, we found that the scaffold proteins Dact1 and Dact2, which are expressed in pre-migratory NC cells, are required for NC delamination in Xenopus and chick embryos, whereas they do not affect the motile properties of migratory NC cells. (ucl.ac.uk)
  • XTcf-3 transcription factor mediates β-catenin-induced axis formation in Xenopus embryos. (esmed.org)
  • Secondly, β-catenin participates in the Wnt signaling pathway as a downstream target. (wikipedia.org)
  • While the pathway is very detailed and not completely understood, in general, when Wnt is not present, GSK-3B (a member of the pathway) is able to phosphorylate β-catenin as a result of a complex formation that includes β-catenin, AXIN1, AXIN2, APC (a tumor suppressor gene product), CSNK1A1, and GSK3B. (wikipedia.org)
  • Indeed, we demonstrate that Hmga2 can induce Spemann Organizer cell fates even when β-catenin, a critical effector of the Wnt signaling pathway, is depleted. (xenbase.org)
  • Despite this clearly defined genetic etiology and the strict and unique implication of the Wnt/β-catenin pathway, no specific molecular targets for these tumors have been identified. (xenbase.org)
  • Hyperactivation of the Wnt/β-catenin signaling pathway via truncation of Apc or constitutive activation of β-catenin induces desmoid tumors in X. tropicalis. (xenbase.org)
  • The Wnt pathway mediates the biological processes of the canonical or non-canonical pathway, depending on the involvement of β-catenin in signal transduction. (chemdiv.com)
  • The recently described role of the Wnt/β-catenin pathway in the regulation of immune cell infiltration of microenvironment tumor resumes interest because of its potential impact to rection of immune therapy. (chemdiv.com)
  • This article presents the roles of the Wnt/β-catenin pathway in cancer and current therapeutic strategies involving this pathway. (chemdiv.com)
  • Nussе and Varmus identified components of the Wnt/β-catenin pathway in 1982 in a study of oncogenic breast tumor viral diseases (MMTV) [1]. (chemdiv.com)
  • Our research interests include the study of the cascade of intracellular events that leads to the activation of Wnt/β-catenin signaling, understand its role in cellular processes during the development of the embryo and, finally, determine the role of the Wnt/β-catenin signaling pathway during organ development and maintenance, such as heart formation. (bu.edu)
  • The Wnt pathway activates target genes by controlling the β-catenin-T-cell factor (TCF) transcriptional complex during embryonic development and cancer. (xenbase.org)
  • Hyperactivation of the Wnt/β-catenin signaling pathway due to mutations in its components initiates the majority of colorectal cancer (CRC) cases and promotes CRC development. (esmed.org)
  • In the past decade, accumulating evidence revealed that nuclear receptors (NRs) modulated Wnt/β-catenin signaling activity via binding to diverse members of this pathway. (esmed.org)
  • Wogonin induced G1 cell cycle arrest by regulating Wnt/β-catenin signaling pathway and inactivating CDK8 in human colorectal cancer carcinoma cells. (esmed.org)
  • LncRNA UCA1 promotes cell proliferation, invasion and migration of laryngeal squamous cell carcinoma cells by activating Wnt/β-catenin signaling pathway. (otwobio.com)
  • In the Drosophila embryo, apical constriction in a strip of epithelial cells along the ventral midline results in the folding of the tissue and the internalization of ventral cells, forming a ventral furrow (VF). (nature.com)
  • Here, we identify Pins-mediated planar cell polarized divisions in several of the mitotic domains of the early Drosophila embryo. (elifesciences.org)
  • Keratinocytes engineered to not express alpha-catenin have disrupted cell adhesion and activated NF-κB. (wikipedia.org)
  • In plakoglobin arm repeats bind alpha-catenin and N-cadherin. (embl.de)
  • RNA was isolated from stage 18 control embryos or embryos depleted of Rspo2 . (xenbase.org)
  • Note the reduction of muscle birefringence in the embryos injected with UM (E) and SM (F). (G-H) Confocal saggital sections of the trunk muscle at the level of anterior somites 7-9 in control embryos (left) and morphants (right). (zfin.org)
  • A) foxq2 , fzl5/8, and six3 expression in 32-cell and late blastula-stage control embryos (Aa-d), ΔFzl5/8 mRNA-injected embryos (Af-i), and embryos treated with JNK inhibitor (Ak-n). (echinobase.org)
  • A) foxq2 expression at stages from 32-cell to hatched blastula, as well as fzl5/8 and six3 expression at mesenchyme blastula stage in control embryos (Aa-f), Fzl1/2/7 morpholino-injected embryos (Ah-m), and embryos treated with PKC inhibitor (Ao-t). (echinobase.org)
  • Catenins play roles in cellular organization and polarity long before the development and incorporation of Wnt signaling pathways and cadherins. (wikipedia.org)
  • β-catenin acts by anchoring the actin cytoskeleton to the junctions, and may possibly aid in contact inhibition signaling within the cell. (wikipedia.org)
  • Mink1 regulates canonical Wnt signaling to define the cell fates of the Spemann Organizer and the Left - Right Organizer , a ciliated structure that breaks bilateral symmetry in the vertebrate embryo . (xenbase.org)
  • Recent work by his group has shown Wnt-β-catenin signaling determines whether a tail or a head will form during regeneration in planarians. (asu.edu)
  • VCAM1 affects the NSC fate by signaling through its intracellular domain to regulate β-catenin signaling in a context-dependent manner. (stanford.edu)
  • The standard model of Wnt signaling specifies that after receipt of a Wnt ligand at the membranous receptor complex, downstream mediators inhibit a cytoplasmic destruction complex, allowing β-catenin to accumulate in the cytosol and nucleus and co-activate Wnt target genes. (silverchair.com)
  • The roles of b-catenin are 'classically' defined: as an adhesion protein and as a signaling protein, transducing extracellular signals to the nucleus to modify gene expression. (chemdiv.com)
  • β -catenin has many binding partners that mediate a diverse set of cellular functions, and the protein probably acts as a 'hub' on which many cellular signaling networks impinge. (chemdiv.com)
  • Wnt/β-catenin signaling is a branch of a functional network that is involved in a broad range of biological systems including stem cells, embryonic development and adult organs. (chemdiv.com)
  • Deregulation of components involved in Wnt/β-catenin signaling has been implicated in a wide spectrum of diseases including a number of cancers and degenerative diseases. (chemdiv.com)
  • Speaking more specifically, β-catenin is an integral structural component of cadherin-based adhesive junctions as well as it a key nuclear effector of canonical in nucleus Wnt signaling. (chemdiv.com)
  • An imbalance in the structural and signaling properties of β-catenin often leads to disease and unregulated growth associated with cancer and metastasis. (chemdiv.com)
  • β-catenin is a component of the complex cadherin complex whose stabilization is required to activate Wnt/β-catenin signaling. (chemdiv.com)
  • Our laboratory studies Wnt/β-catenin signaling , one of the signaling pathways that are required for the development of organs and tissues in the embryo and for the maintenance of tissues in the adult. (bu.edu)
  • Identifying key molecular steps in Wnt/β-catenin signaling will help develop novel or improved strategies for the treatment of diseases that involve Wnt/β-catenin signaling. (bu.edu)
  • A faithful Wnt/β-catenin reporter that demonstrates Wnt/β-catenin signaling in embryos. (bu.edu)
  • Upon Wnt signaling, ß-catenin binds and activates nuclear TCFs by providing a trans-activation domain. (hubrecht.eu)
  • Integration of canonical and noncanonical Wnt signaling pathways patterns the neuroectoderm along the anterior-posterior axis of sea urchin embryos. (echinobase.org)
  • Remarkably, the anterior neuroectoderm (ANE) of the deuterostome sea urchin embryo expresses many of the same transcription factors and secreted modulators of Wnt signaling, as does the early vertebrate ANE (forebrain/eye field). (echinobase.org)
  • Moreover, as is the case in vertebrate embryos, confining the ANE to the anterior end of the embryo requires a Wnt/β- catenin -dependent signaling mechanism. (echinobase.org)
  • B) qPCR measurements from three different cultures of embryos showing that ANE regulatory genes are up-regulated at the late blastula stage (24 hpf) in the absence of functional Fzl5/8 signaling. (echinobase.org)
  • Unphosphorylated β-catenin accumulates in the nuclear and interacts with TCF/LEF factors to stimulate the transcription of the downstream target genes of Wnt/β-catenin signaling. (esmed.org)
  • Therefore, the suppression of dysregulated Wnt/β-catenin signaling is considered as a promising strategy for CRC therapy. (esmed.org)
  • In this review, we mainly focus on the regulation and the underlying mechanisms of Wnt/β-catenin signaling by NRs and their ligands or pharmacological modulators. (esmed.org)
  • A double-negative feedback loop between Wnt-β-catenin signaling and HNF4α regulates epithelial-mesenchymal transition in hepatocellular carcinoma. (esmed.org)
  • Canonical WNT signals are transduced through Frizzled family receptors and LRP5/LRP6 coreceptor to the β-catenin signaling cascade. (aacrjournals.org)
  • Canonical ß-catenin signaling is responsible for dermis initiation in the embryo. (lifemapsc.com)
  • Dact1/2 inhibit Wnt/β-catenin signalling upstream of the transcriptional activity of T cell factor (TCF), which is required for EMT to proceed. (ucl.ac.uk)
  • Swiss Mouse Embryo Fibroblast Cells with MitoTracker Red CMXRos, Alexa Fluor 488, and DAPI - A log phase culture of embryonic Swiss mouse fibroblast cells was stained with MitoTracker Red CMXRos, Alexa Fluor 488 conjugated to phalloidin, and DAPI, which target the intracellular mitochondrial network, cytoskeletal actin filaments, and nuclei, respectively. (fsu.edu)
  • Mouse Embryo Fibroblast Cells with Texas Red-X, Alexa Fluor 488, and DAPI - In this section, a Swiss mouse embryo fibroblast cell is presented that was resident in a culture labeled with the fluorophore Texas Red-X conjugated to wheat germ agglutinin, a fluorescent lectin that selectively binds to sialic acid residues. (fsu.edu)
  • Molecular studies in mouse embryo fibroblast and human colorectal cancer cell lines defined APC/β-catenin and SRC-PI3K as key pathways in miR-135b activation. (gla.ac.uk)
  • In our research, we use the zebrafish embryo to investigate how intercellular Wnt protein transport is regulated and how signals are subsequently delivered to the target cell in a living vertebrate organism. (exeter.ac.uk)
  • Vertebrate catenins contain a large number of Arm-domains including 3 subfamilies: β-tencains, p120 and plakophilins 1 and 3. (chemdiv.com)
  • Biological effects of power frequency magnetic fields: Neurochemical and toxicological changes in developing chick embryos med. (emf-portal.org)
  • The effects of exposure of chicken embryos to a 50 Hz magnetic field on neurotransmitter levels in the brain and tissue damage should be investigated. (emf-portal.org)
  • The authors conclude that exposure of chicken embryos to a 50 Hz magnetic field could affect noradrenaline and glutamate levels in the brain . (emf-portal.org)
  • To clarify the roles of the egg yolk as a source of THs, the TH content in the yolk and the expression of TH regulator genes in the yolk sac membrane were evaluated throughout the 21-day incubation period of chicken embryos. (edu.au)
  • In APC-deficient colon carcinoma cells, we demonstrated that ß-catenin accumulates and is constitutively complexed with the TCF family member TCF4, providing a molecular explanation for the initiation of colon cancer (5). (hubrecht.eu)
  • Mice lacking plakoglobin have cell adhesion defects in many tissues, although β-catenin substitutes for plakoglobin at many cellular junctions. (wikipedia.org)
  • The protocol is suitable for studying the development of hPSCs in mouse embryos and may facilitate the generation of human cells, tissues and organs in animals. (nature.com)
  • It has been postulated that Wnt/β-catenin signalling forms concentration gradients across responsive tissues and act as morphogens. (exeter.ac.uk)
  • In 1963, Joseph A. DiPaolo, working in the US, discussed various birth defects found in mice fetuses whose mothers were fed Thalidomide daily, but he found only one kind of anamoly called fetal resorption, or the partial or complete dissolution of fetal tissues after some embryos had died in utero . (asu.edu)
  • F9 cells were genetically engineered to lack β-catenin, resulting in increased association of plakoglobin with E-cadherin. (wikipedia.org)
  • In F9 cells lacking both β-catenin and plakoglobin, very little E-cadherin and α-catenin accumulated at the cell surface. (wikipedia.org)
  • CDMG treatment of amputated zebrafish hearts reduces nuclear β-catenin in injured heart tissue, increases cardiomyocyte (CM) proliferation, and expedites wound healing, thus accelerating cardiac muscle regeneration. (vanderbilt.edu)
  • Further Pkp-catenin actions in the nuclear area have grown to be of increased curiosity using the recognition of relationships between Pkp2-catenin and RNA Pol III and Pkp1 with single-stranded DNA. (healthyconnectionsinc.com)
  • As released so far Pkp nuclear organizations possess intriguingly included Pkp1-catenin binding to single-strand DNA and Pkp2-catenin towards the RNA polymerase III holoenzyme [26] [35]. (healthyconnectionsinc.com)
  • In offering an initial evaluation of their nuclear romantic relationship this function should help out with furthering a knowledge of how Pkp-catenins impact development and perhaps disease. (healthyconnectionsinc.com)
  • We have combined zebrafish embryo-based screens with cardiomyogenesis assays to discover selective small molecules that modulate heart development and regeneration with minimal adverse effects. (vanderbilt.edu)
  • We characterize the activation kinetics of these reagents in vitro and demonstrate their efficacy in zebrafish embryos that express NfsB either ubiquitously or in defined cell populations. (cdc.gov)
  • Here we use morpholino- or dominant negative-mediated interference to demonstrate that the early sea urchin embryo integrates information not only from Wnt/β- catenin but also from Wnt/Fzl5/8- JNK and Fzl1/2/7-PKC pathways to provide precise spatiotemporal control of neuroectoderm patterning along its AP axis. (echinobase.org)
  • β-Catenin binds directly to the cytoplasmic tail of classical cadherins. (wikipedia.org)
  • The tumor suppressor protein APC forms the core of a cytoplasmic complex which binds ß-catenin and targets it for degradation in the proteasome. (hubrecht.eu)
  • We reveal that Pkp3-catenin binds to ETV1 an associate of the bigger E-twenty six (Ets) family members whose people bind DNA straight (e.g. with Cefdinir a conserved Ets site) (evaluated in Hollenhorst et al. (healthyconnectionsinc.com)
  • E) Embryo coexpressing Wnt8 DNA and Rspo2 mRNA. (xenbase.org)
  • While their cytoplasmic features are less realized Pkp-catenins get excited about regulating mRNA manifestation the trafficking of desmosomal cadherins and insulin-induced cell proliferation [20]-[23]. (healthyconnectionsinc.com)
  • C) foxq2 expression is completely eliminated in embryos injected with either Wnt1 (b) or Wnt8 (d) mRNA. (echinobase.org)
  • Using embryos and mammalian cells we offer evidence for the Pkp3:ETV1 complex on both functional and biochemical amounts. (healthyconnectionsinc.com)
  • 16] Tetsu O, McCormick F. β-Catenin regulates expression of cyclin D1 in colon carcinoma cells. (esmed.org)
  • Eventually, some of this accumulated β-catenin will move into the nucleus with the help of Rac1. (wikipedia.org)
  • Visualizing Structural Features of the Golgi Complex and Nucleus in Swiss Mouse Embryo Cells - In order to examine structural features of the Golgi complex and nucleus at relatively high magnification, a log-phase culture of 3T3 cells was fixed, permeabilized, blocked with normal goat serum, and then treated with rabbit anti-giantin (Golgi protein) primary antibodies followed by goat anti-rabbit secondary antibodies (IgG) conjugated to Alexa Fluor 568. (fsu.edu)
  • Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells. (wikipedia.org)
  • This library represents a selection of drug-like compounds aimed at modulating protein-protein interaction (PPI) of β-catenin with different proteins involved in significant physiological processes. (chemdiv.com)
  • The molecular weight of proteins were 102, 88 and 80 kDa, so they were named α, β and γ-catenin. (chemdiv.com)
  • At this point, β-catenin becomes a coactivator for TCF and LEF to activate Wnt genes by displacing Groucho and HDAC transcription repressors. (wikipedia.org)
  • the tadpoles showed reduced expression of cα(E)-catenin , small muscle protein, dystrophin , and myosin light chain genes. (biomedcentral.com)
  • Unlike the other E(spl)-C genes, the gene is expressed within neuronal cells in the embryo. (sdbonline.org)
  • Expression of thyroid hormone regulator genes in the yolk sac membrane of the developing chicken embryo [1] "Thyroid hormones (THs) are essential for the correct development of nearly every structure in the body from the very early stages of development, yet the embryonic thyroid gland is not functional at these stages. (edu.au)
  • α-catenin β-catenin γ-catenin δ-catenin All but α-catenin contain armadillo repeats. (wikipedia.org)
  • Such DNA constructs, when electroporated in fertilised eggs, produce transiently transgenic embryos and allow rapid gene functional and gene regulatory analyses , in precisely identifyable tissue precursors, from earliest embryonic stages in vivo . (uibk.ac.at)
  • Using two in vivo models, we show that Wnt/β-catenin signalling is transiently inhibited at the time of NC delamination. (ucl.ac.uk)
  • Fig. 7: NGS quantification of human DNA in mouse-human chimeric embryos. (nature.com)
  • E) Quantification of abnormal cardiac OFT looping in embryos with overexpression of wild-type and kinase-dead human MINK1 RNA. (xenbase.org)
  • A) Representative images of foxj1 expression in stage 10.5 uninjected embryos and mink1 crispants along with quantification. (xenbase.org)
  • F9 embryonal carcinoma cells are similar to the P19 cells shown in Figure 1 and normally have cell-to-cell adhesion mediated by E-cadherin with β-catenin bound to the cytoplasmic domain of E-cadherin. (wikipedia.org)
  • While less attention is directed at α-catenin in studies involving cell adhesion, it is nonetheless an important player in cellular organization, function and growth. (wikipedia.org)
  • β-Catenin is a fascinating protein with many important cellular and developmental functions. (chemdiv.com)
  • Whats more, ascidian embryos develop with a fixed cellular lineage based on few and large cells allowing cellular resolution of cell fate identity. (uibk.ac.at)
  • We have used conditional gene deletion of mouse β-catenin in Sox17-positive embryonic and extra-embryonic endoderm as well as vascular endothelial progenitors to address the function of canonical Wnt signalling in cell lineage formation and patterning. (biologists.com)
  • Embryonic defects are much more extreme in germline clones, where the majority of mutant embryos die during embryogenesis and do not form cuticle, implying a strong chem maternal contribution. (peerj.com)
  • For instance, when an epithelial layer is complete and the adherens junctions indicate that the cell is surrounded, β-catenin may play a role in telling the cell to stop proliferating, as there is no room for more cells in the area. (wikipedia.org)
  • These gene products are important in determining cell fates during normal development and in maintaining homeostasis, or they can lead to de-regulated growth in disorders like cancer by responding to mutations in β-catenin, APC or Axin, each of which can lead to this de-regulated β-catenin level stabilization in cells. (wikipedia.org)
  • Mice engineered to specifically have vascular endothelium cells deficient in β-catenin showed disrupted adhesion between vascular endothelial cells. (wikipedia.org)
  • Naive human pluripotent stem cells (hPSCs) can be used to generate mature human cells of all three germ layers in mouse-human chimeric embryos. (nature.com)
  • Established naive hPSCs are injected into mouse blastocysts, which produce E17.5 mouse embryos containing 0.1-4.0% human cells as quantified by next-generation sequencing of 18S ribosomal DNA amplicons. (nature.com)
  • Transient inhibition of mTOR in human pluripotent stem cells enables robust formation of mouse-human chimeric embryos. (nature.com)
  • Our findings provide new insight on how stem cells in the embryo are preserved to meet the need for growth and regeneration. (stanford.edu)
  • We find that Cardiomogen acts as a Wnt inhibitor by targeting β-catenin and reducing Tcf/Lef-mediated transcription in cultured cells. (vanderbilt.edu)
  • Delamination of neural crest (NC) cells is a bona fide physiological model of epithelial-to-mesenchymal transition (EMT), a process that is influenced by Wnt/β-catenin signalling. (ucl.ac.uk)
  • Together, these data identify a novel yet important regulatory element that inhibits β-catenin signalling, which then affects NC delamination. (ucl.ac.uk)
  • The y-axis shows the fold change in gene expression in ΔFzl5/8-containing embryos relative to controls. (echinobase.org)
  • Established by George Todaro and Howard Green in 1962 from disaggregated Swiss mouse ( Mus musculus ) embryo tissue, the 3T3 cell line is a standard fibroblast cell line used in a wide spectrum of research and industrial biomedical applications. (fsu.edu)
  • Injured hearts exposed to CDMG1 display increased newly formed CMs and reduced fibrotic scar tissue, which are in part attributable to the β-catenin reduction. (vanderbilt.edu)
  • Patterning the neuroectoderm along the anterior-posterior (AP) axis is a critical event in the early development of deuterostome embryos. (echinobase.org)
  • The endomesoderm gene regulatory network (GRN) models the transcriptional control system defining vegetal specification of the sea urchin( Strongylocentrotus purpuratus ) embryo during the first 30 hours of development. (biologists.com)
  • Recording and contextualizing the science of embryos, development, and reproduction. (asu.edu)
  • β-catenin is a multipurpose and evolutionarily conserved molecule that plays a critical role by metazoans in a variety of processes in development and homeostasis. (chemdiv.com)
  • Like the original allele, a fraction of mutant embryos die with cuticular defects, notably head involution and dorsal closure defects. (peerj.com)
  • A fraction of these mutant embryos have lethal head involution or dorsal open phenotypes, and have an extended phenocritical period. (peerj.com)
  • Four-cell embryos were injected animally into both dorsal blastomeres with the indicated constructs and cultured to stage 38. (xenbase.org)
  • H) Dorsal marginal zones (DMZ) were dissected at stage 10 from the control and Rspo2 RNA- or RMOATG-injected embryos and cultured until stage 12. (xenbase.org)
  • A representative example of a 'druggable' 'hot spots' included specific topological features of the β -catenin interaction. (chemdiv.com)
  • Functional interaction of β-catenin with the transcription factor LEF-1. (esmed.org)
  • Our function uses both embryos and mammalian cell-line centered assays and helps the discussion of Pkp3-catenin with ETV1 on both biochemical and practical grounds. (healthyconnectionsinc.com)
  • Satou, Y. (2020): Gata is ubiquitously required for the earliest zygotic gene transcription in the ascidian embryo. (uibk.ac.at)
  • In this research we display for the very first time to our understanding a Pkp-catenin particularly interacts having a transcription element. (healthyconnectionsinc.com)
  • Altogether we record the first discussion of the plakophilin-catenin having a site-specific DNA-binding transcription element specifically Pkp3-catenin's association with ETV1. (healthyconnectionsinc.com)
  • Rothbächer, U. (2016): Embryo Microinjection and Electroporation in the Chordate Ciona intestinalis. (uibk.ac.at)
  • Satou, Y. (2016): A Maternal System Initiating the Zygotic Developmental Program through Combinatorial Repression in the Ascidian Embryo. (uibk.ac.at)
  • Tetraploid rescue experiments reveal that lack of β-catenin in the anterior visceral endoderm results in defects in head organizer formation. (biologists.com)
  • Birth Defects Research Part C: Embryo Today: Reviews. (esmed.org)
  • The biomedical accomplishment of human in vitro fertilization and embryo transfer (IVF-ET) took years to become the successful technique that presently enables infertile couples to have their own children. (asu.edu)
  • These complexes, which help regulate cell growth in addition to creating and maintaining epithelial layers, are known as adherens junctions and they typically include at least cadherin, β-catenin, and α-catenin. (wikipedia.org)
  • β-catenin is particularly interesting as it plays a dual role in the cell. (wikipedia.org)
  • Sox17 lineage tracing in the definitive endoderm (DE) shows a cell-autonomous requirement for β-catenin in midgut and hindgut formation. (biologists.com)
  • A,B) Four-cell embryos were injected with 0.5 ng of Rspo2 RNA into both animal- ventral blastomeres and cultured until stage 28. (xenbase.org)
  • Unexpectedly, shortly after Wnt treatment, we detected the dephosphorylated form of β-catenin at the plasma membrane, where it displayed a discontinuous punctate labeling. (silverchair.com)
  • It is assumed that the chicken yolk sac inactivates THs contained abundantly in the yolk and supplies the hormones to the developing embryo in appropriate concentrations until the second week of incubation, while THs may be activated in the yolk sac membrane in the last week of incubation. (edu.au)
  • The foxa gene is an integral component of the endoderm specification subcircuit of the endomesoderm gene regulatory network in the Strongylocentrotus purpuratus embryo. (biologists.com)