This video guide is an essential learning tool for investigators considering work with Xenopus eggs and embryos. (cshlpress.com)
Studies using Xenopus cell-free extracts, oocytes, eggs, embryos, larval stages, and adult frogs have yielded important insights into a multitude of key biological processes-from mechanisms underlying the cell cycle to embryonic development to human disease. (cshlpress.com)
To examine the role of the brain during early development, the researchers removed the brains of Xenopus laevis frog embryos 27-1/2 hours after the eggs were fertilized, long before independent embryonic activity occurs. (medindia.net)
Chk1 is also transiently activated at the MBT in Xenopus laevis embryos, even when there is no block to DNA replication or damaged DNA. (vt.edu)
Whole mount in situ hybridisation (Wmish) of wild type Xenopus laevis embryos at different developmental stages. (xenbase.org)
For a reverse experiment, DNA methylation in early Xenopus embryos was assessed by MBD affinity capture. (biomedcentral.com)
Together, these results suggest that xCyp26c plays a specific role in anterior-posterior (A-P) neural patterning of Xenopus embryos. (molcells.org)
Xenopus laevis embryos were incubated in four experimental solutions and a control of aged tap water. (saintpeters.edu)
To evaluate the impacts of Masp 1 genes on Xenopus laevis embryos through Bmp4 and Otx2 genes- BMp4genes are primarily used to signal cells to form a cement gland, whereas it expressed Otx2genes through the instruction of cement gland formation- we compared their function through in-situ hybridization and the consequential expression through embryotic stages 16-18 and 19. (boisestate.edu)
Oocyte2
Endogenous MeCP2 and MBD3 were precipitated from Xenopus oocyte extracts and conditions for methylation-specific binding were optimized. (biomedcentral.com)
The Xenopus oocyte possesses a large maternal store of ribosomes, thereby uncoupling early development from the de novo ribosome biosynthesis required for cell growth. (silverchair.com)
Epidermis5
Here we report the essential role of NO during embryonic epidermis development. (labgenexp.eu)
Xenopus embryonic epidermis has become a useful model reflecting human epithelial tissue composition. (labgenexp.eu)
The developing epidermis of Xenopus laevis is formed from specialized ionocytes, multi-ciliated, goblet and small secretory cells. (labgenexp.eu)
Figure 4: miR-34/449 deficiency causes defective ciliogenesis in the Xenopus embryonic epidermis. (nature.com)
We examined the bioelectric properties (resting potential gradients in the epidermis) of Xenopus laevis froglets undergoing hindlimb amputation and observed that the contralateral (undamaged) limb exhibits apparent depolarization signals immediately after the opposite hindlimb is amputated. (biologists.com)
Development18
This 3-volume video set contains 68 demonstrations of the manipulation of living eggs using techniques taught in the course on the Early Development of Xenopus laevis held at Cold Spring Harbor Laboratory, New York, for the past four years. (cshlpress.com)
Xenopus laevis embryonic protein UVS.2, which is expressed during dorsoanterior development. (embl.de)
In another lifetime, if they had been allowed to follow their natural development, the stem cells taken from embryonic frogs would have turned into skin and heart tissue within living, breathing animals. (sciencealert.com)
This is according to new research from Tufts University scientists which shows that long before movement or other behaviors occur, the brain of an embryonic frog influences muscle and nerve development and protects the embryo from agents that cause developmental defects. (medindia.net)
Mammalian neural crest cells are also ideal models because they have a key role in embryonic facial development. (neurosciencenews.com)
In Xenopus laevis, early embryonic development consists of twelve rapid cleavage cycles between DNA replication (S) and mitosis (M) without checkpoints or gap phases. (vt.edu)
Chk1 plays a critical but not fully understood role in cell cycle remodeling and early embryonic development. (vt.edu)
The regeneration of a tissue intuitively recapitulates aspects of its embryonic development. (frontiersin.org)
RMND5 from Xenopus laevis is an E3 ubiquitin-ligase and functions in early embryonic forebrain development. (xenbase.org)
We therefore suggest that Xenopus laevis Rmnd5 , as a subunit of the CTLH complex, is a ubiquitin-ligase targeting an unknown factor for polyubiquitination and subsequent proteasomal degradation for proper fore- and midbrain development. (xenbase.org)
rmnd5 is expressed during early embryonic development. (xenbase.org)
In this study, we demonstrate that PPan is expressed maternally as well as in the eye and cranial neural crest cells (NCCs) during early Xenopus laevis development. (silverchair.com)
The research aims to study the effects of 5 ug/ml and 10 ug/ml caffeine, 0.01% coffee, and 0.01% decaffeinated coffee on neurulation and brain development in Xenopus laevis (clawed frog). (saintpeters.edu)
The mutations of Masp 1/3 genes, among humans and the Xenopus laevis species, have impacted the rate of embryonic development and craniofacial structures. (boisestate.edu)
These discoveries may account that the Masp1 gene accounts for the mutations responsible for the embryonic development of the Xenopus laevis species that is synonymous with humans with 3MC Syndrome. (boisestate.edu)
Tolloid (Tld) metalloproteases are zinc dependent extra-cellular endopeptidases that have numerous roles during embryonic development. (ucl.ac.uk)
I have also identified three members of the lox family and characterise their role during early Xenopus development. (ucl.ac.uk)
Frog3
Here, we document the interplay between rhythmic locomotory and `C-start' escape swimming in Xenopus frog larvae when exposed to hyperthermic conditions. (biologists.com)
Members of the frog Xenopus genus are versatile model organisms used in developmental biology, cell biology, neuroscience, and toxicology research, among others. (cshlpress.com)
To identify genes involved in the anterior neural specification, we analyzed gene expression profiles using a Xenopus Affymetrix Gene Chip after BMP-4 inhibition in animal cap explants. (molcells.org)
Cyp26 genes have been previously characterized in various species, but their function in Xenopus leavis have not yet been fully identified. (molcells.org)
The frogs Xenopus laevis and Xenopus tropicalis undergo temporally restricted regenerative healing of appendage amputations and spinal cord truncations, injuries that are both devastating and relatively common in human patients. (frontiersin.org)
Frogs2
Then, the team selected the most promising designs to physically build out of cells harvested from embryonic African clawed frogs ( Xenopus laevis ). (sciencealert.com)
It is a critical reference for laboratories that currently work with these organisms and will serve as an essential start-up guide for those seeking to start using Xenopus frogs in their research for the first time. (cshlpress.com)
Tissues2
Here, we performed a discriminant microarray analysis using Xenopus laevis genome arrays to compare tail tissues of control and predator-exposed tadpoles. (biomedcentral.com)
Ahrens K, Schlosser G (2005) Tissues and signals involved in the induction of placodal Six1 expression in Xenopus laevis . (springer.com)
Developmental2
Application of various small molecules and recombinant proteins to mouse embryonic stem cells at specific time points in vitro has enabled recapitulation of developmental cues with subsequent formation of inner ear organoids. (springer.com)
For over a century amphibians from the genus Xenopus have been the organisms of choice to study developmental processes. (biomedcentral.com)
Patterning1
It is well-established that early embryonic signaling pathways are critical for growth and patterning of new tissue during regeneration. (frontiersin.org)
In this thesis I identify and describe the expression for Xenopus homologues of procollagen 3al, 5al, 5a2 and Hal, biglycan and a laminin ct-chain like gene. (ucl.ac.uk)
Immunology1
There are protocols for analyses of Xenopus immunology, neurobiology, metamorphosis, and adult physiology. (cshlpress.com)
Other types of cell lines are also available in X. laevis including: fibroblasts lines of the different strains and LG clones, and a kidney cells line (A6). (rochester.edu)
In both mouse and Xenopus , miR-34/449-deficient multiciliated cells (MCCs) exhibited a significant decrease in cilia length and number, due to defective basal body maturation and apical docking. (nature.com)
C) Localization of Homo sapiens RMND5A ( RMND5a , middle panel), RMND5B (RMND5b, bottom panel) and Xenopus laevis Rmnd5 ( Rmnd5 , top panel) in HEK293 cells. (xenbase.org)
Protein2
In a recent, elegant study, Inomata and colleagues proposed a new model explaining how bone morphogenic protein (BMP) activity gradient scales with embryo size in the early Xenopus laevis embryo. (huji.ac.il)
Xenopus laevis Rmnd5 protein is structurally and functionally related to human RMND5A . (xenbase.org)
Amphibian2
X. laevis is the only amphibian where true lymphoid tumors have been discovered and cell lines have been obtained opening new avenues for tumor biology and tumor immunity. (rochester.edu)
Cloning and functional characterization of two key enzymes of glycosphingolipid biosynthesis in the amphibian Xenopus laevis. (xenbase.org)
Stages1
The role of Cyp26 during RA utilization and the anteriorization of the neuroectoderm during the early embryonic stages of Xenopus is also unclear. (molcells.org)
Here we review recent insights into the biophysical, biochemical, and epigenetic processes that underlie regenerative healing in amphibians, focusing particularly on tail and limb regeneration in Xenopus . (frontiersin.org)
This temporally restricted regenerative competence therefore makes Xenopus an appealing model for defining the features that enable or inhibit regenerative healing. (frontiersin.org)