Embryonic stem cloning and stem cell. Medical search. Frequent questions

Scientists announced this week that they have successfully produced embryonic stem cells by transferring the DNA of human skin cells into unfertilized human eggs to produce embryos, a technique the Church considers to be an abuse of human life. (womenofgrace.com)
Rossant, J. & Vijh, K. M. Ability of outside cells from preimplantation mouse embryos to form inner cell mass derivatives. (nature.com)
Depending on the source, stem cells can be classified into two broad categories i.e. embryonic stem cells that are derived from embryos and non-embryonic stem cells that are derived from adult and fetal tissues. (benthamscience.com)
Under such circumstances, the idea of "therapeutic cloning" was proposed, indicating the generation of ESCs from SCNT embryos for therapeutic purpose. (benthamscience.com)
However, the derivation of human NT-ESCs goes with the destruction of clone embryos, leading to fierce ethical disputes. (benthamscience.com)
And now Washington joins the infamous list with Senate Bill 5594, a thoroughly disingenuous piece of legislation that purports to outlaw the cloning of human beings, but by manipulating language and redefining terms, actually permits human cloning and gestation of the resulting cloned embryos through the ninth month. (cbc-network.org)
Embryonic stem cells are derived from early embryos and have the ability to differentiate into any cell type. (spiked-online.com)
For example, the same technology used to eliminate disease-causing genes or to clone embryos may eventually be deployed to produce genetically engineered children. (ssrn.com)
The legislation, which bans reproductive cloning as well as embryonic stem cell research in which human life is destroyed, was amended in the House last week to outlaw the cloning process to create human embryos for research, but would allow importation of embryonic stem cell lines created outside the state for research. (lifenews.com)
The researchers say the finding, which has been successfully demonstrated in frog embryos, will help scientists control the differentiation of various cell types. (scitechdaily.com)
When Notum was not present, the embryos would become a sack of skin cells with no head and a tiny brain, a result of embryonic progenitor cells making only epidermal but not neural cells. (scitechdaily.com)
TORONTO (CNS) - The international scientific body governing stem cell research is abandoning the absolute 14-day limit on culturing human embryos in the laboratory, putting pressure on Canada's law prohibiting the practice. (thetablet.org)
On May 26, the International Society for Stem Cell Research said it was relaxing the 14-day rule, which prohibited experiments on human embryos past 14 days of development in the lab. (thetablet.org)
Recent experimentation that has cultured lab-grown monkey embryos for up to 20 days and the possibility of creating human-monkey chimeras - beings that contain genetic codes from two different species - has further pushed the envelope on embryonic stem cell research. (thetablet.org)
The Australian government has issued its first license for cloning human embryos to obtain embryonic stem cells. (bioedge.org)
Chinese scientists have successfully created chimeric embryos containing a combination of human and pig cells. (bioedge.org)
It is further speculated that those who support human embryonic stem cell research are also seeking human embryos for the purposes of human cloning. (all.org)

Therapeutic Use of Cloning: Osmangazi Tu. (yildiz.edu.tr)
There are important problems in the therapeutic use of cloning which increase with the more advanced differentiation of somatic cells. (yildiz.edu.tr)
5. In 2001, France and Germany requested the United Nations General Assembly to develop international conventions on human reproductive cloning, therapeutic cloning and research on stem cells. (who.int)
Kuldip S. Sidhu , " Frontiers in Pluripotent Stem Cells Research and Therapeutic Potentials Bench-to-Bedside ", Bentham Science Publishers (2012). (benthamscience.com)
Embryonic stem cells (ESCs) can grow infinitely and give rise to all types of cells in human body, thus of tremendous therapeutic potentials for a variety of diseases, such as Parkinson's disease, spinal cord injury, and diabetes. (benthamscience.com)
If the cloned human organism is to be experimented upon and destroyed, the process is often called "therapeutic cloning. (cbc-network.org)
A well-respected medical ethicist from one of Canada's leading universities says Canada must not legalize embryonic cloning for any purpose, including therapeutic purposes. (catholicnewsagency.com)
The reader benefits from the scholar's clear explanation about embryonic stem-cell research and therapeutic cloning. (catholicnewsagency.com)
A renewable, tissue culture source of human cells capable of differentiating into a wide variety of cell types would have broad applications in basic research and therapeutic techniques. (spiked-online.com)
One of the most promising techniques is what is known as therapeutic cloning. (spiked-online.com)
Reproductive cloning in humans and therapeutic cloning in primates: is the ethical debate catching up with the recent scientific advances? (bmj.com)
Human cloning ought to be banned, both reproductive cloning and so-called therapeutic cloning-or as Stanford University recently referred to it, "human nuclear transplantation. (probe.org)
Recombinant DNA technology, Embryonic stem cells, and therapeutic cloning. (edu.sa)
So far, public attention to the new health care has focused on two of its methods: embryonic stem-cell research and therapeutic cloning. (ssrn.com)
If research involving human embryonic stem cells (ESCs) is to achieve its potential for creating breakthrough medical therapies, additional new cell lines should be created, and therapeutic cloning--or somatic cell nuclear transfer (SCNT)--should be employed, according to an expert panel of the National Academy of Sciences. (the-scientist.com)
An Australian ban on therapeutic cloning was lifted in December 2006 after a long debate in Federal parliament. (bioedge.org)
Yet, human embryonic stem cell research has thus far been unsuccessful in the quest to develop any therapeutic treatments. (all.org)
With this background information as a foundation, we then discuss each of the key questions in relation to the upcoming therapeutic trial and critically assess if the time is ripe for clinical translation of parthenogenetic stem cell technology in Parkinson's disease. (lu.se)

Stem cells are not specialized and the process of their specialization is called differentiation. (benthamscience.com)
The exact process of differentiation is not yet understood and although embryonic stem cells can, in principle, provide for all human tissue, scientists are some way from controlling the process. (spiked-online.com)
The expression of TRA-1-60 antigen is stage-specific and can be used to characterize embryonic cells and monitor their differentiation. (bdbiosciences.com)
As human EC and ES cells undergo differentiation, expression of TRA-1-60 antigen is lost. (bdbiosciences.com)
miRNA 34a, 100, and 137 modulate differentiation of mouse embryonic stem cells. (genscript.com)
Genetic abnormalities associated with MDS block differentiation of hematopoietic stem and progenitor cells. (medscape.com)
Aim: To detect the expression of molecules associated with Notch signaling pathway in stem cells from human exfoliated deciduous teeth (SHED) cultured in specific differentiation medium, namely, keratinocyte growth medium (KGM). (bvsalud.org)
Notch-1, Jagged-1, Jagged-2, and stem cell marker Nanog are expressed in SHED cultured in KGM which may be involved in the differentiation into epithelial-like cells in human dental pulp tissues. (bvsalud.org)
Since the Notch signaling pathway molecules play an important role in differentiation of epithelial cells, it is important to identify the presence of notch signaling molecules in SHED during the process of cell differentiation. (bvsalud.org)
The Notch signaling pathway provides important intercellular signaling mechanisms essential for cell fate specification and it regulates differentiation and proliferation of stem or progenitor cells by para-inducing effects 3-4 . (bvsalud.org)
Notch signaling pathway is also involved in the regulation of epithelial cell differentiation in various tissues 5-6 . (bvsalud.org)
Stem and progenitor cell populations are often heterogeneous, which may reflect stem cell subsets that express subtly different properties, including different propensities for lineage selection upon differentiation, yet remain able to interconvert. (lu.se)
A key challenge is to understand how state, but must also afford flexibility in cell-fate choice to permit the different cell-fate options confronting stem and progenitor cell-type diversification and differentiation in response to cells are selected and coordinated such that adoption of a given intrinsic cues or extrinsic signals. (lu.se)
Evidence the fate of stem cells has broad ramifications for biomedical suggests that during development or differentiation, cells make science from elucidating the causes of cancer to the use of very precise transitions between apparently stable ``network stem cells in regenerative medicine. (lu.se)
Temporal multimodal single-cell profiling of native hematopoiesis illuminates altered differentiation trajectories with age. (lu.se)

This mouse egg (top) is being injected with genetic material from an adult cell to ultimately create an embryo - and, eventually, embryonic stem cells. (usf.edu)
They look like the cells in a human embryo - in fact, they're called embryonic stem cells. (usf.edu)
2. Nuclear transfer is a technique used to duplicate genetic material by creating an embryo through the transfer and fusion of a diploid cell in an enucleated female oocyte.2 Cloning has a broader meaning than nuclear transfer as it also involves gene replication and natural or induced embryo splitting (see Annex 1). (who.int)
Embryonic stem cells can then be harvested from the cloned embryo and used to create new cells and organs for the original organism. (kottke.org)
The mechanisms underlying the appearance of asymmetry between cells in the early embryo and consequently the specification of distinct cell lineages during mammalian development remain elusive. (nature.com)
Louvet-Vallee, S., Vinot, S. & Maro, B. Mitotic spindles and cleavage planes are oriented randomly in the two-cell mouse embryo. (nature.com)
A little shot of electricity comes next, and if all goes well, a new human cloned embryo comes into being and begins to develop in the same way as a sexually created embryo. (cbc-network.org)
Cloning of a human being" means asexual reproduction by implanting or attempting to implant the product of nuclear transplantation [e.g., an embryo] into a uterus or substitute for a uterus with the purpose of producing a human being. (cbc-network.org)
For instance, he wonders-just an intellectual puzzle, he assures me, that he would never want to do-What would happen if scientists injected human stem cells into a monkey embryo? (discovermagazine.com)
Nearly all the cells in the stalled embryo had turned into brain cells, simply because a single protein had been stopped. (discovermagazine.com)
They instead refer to the embryo as a "clump of cells" or as "potential human life," Somerville says, "despite the fact that they are, given the right conditions, human life with the potential to go on living for years and years, just like all of us. (catholicnewsagency.com)
I have been asked to comment on the latest news that scientists are now able to harvest embryonic stem cells without killing the embryo. (christianliferesources.com)
Specifically, two new procedures are being reported in an effort to avoid the ethical offense of killing the embryo when extracting stem cells. (christianliferesources.com)
It involves extracting a cell from an embryo and then stimulating that cell to produce stem cells. (christianliferesources.com)
It is also our view that there are no sound reasons for treating the early-stage human embryo or cloned human embryo as anything special, or as having moral status greater than human somatic cells in tissue culture. (wikiquote.org)
Ellington, who refused to compromise even at the urging of Gov. Blanco, said his support stems from his views that the embryo is not a human life. (lifenews.com)
We want real cures now available from ethical adult stem cell research, not illusory promises from unproductive embryo research,' the Louisiana Coalition for Ethical Stem Cell Research said in a statement. (lifenews.com)
Obtaining stem cells from a human embryo is highly unethical. (all.org)
There is only one way to obtain stem cells from a developing human embryo, and it involves killing the embryo. (all.org)

2. Over the years, the international community has tried without success to build a consensus on an international convention against the reproductive cloning of human beings. (who.int)
3. Creating awareness among ministries of health in the African Region will provide them with critical and relevant information on the reproductive cloning of human beings and its implications to the health status of the general population. (who.int)
7. The WHO Regional Committee for Africa is invited to review this document for information and guidance concerning reproductive cloning of human beings. (who.int)
3. Media reports on nuclear transfer are usually about one form, reproductive nuclear transfer, also known as reproductive cloning of human beings . (who.int)
If it is to be brought to birth, the process is usually called "reproductive cloning. (cbc-network.org)
Reproductive cloning is expensive and highly inefficient. (wikiquote.org)
Governor Blanco discussed the bills with the Louisiana Coalition for Ethical Stem Cell Research on the final day of the session, and said that bills banning reproductive cloning but allowing for destructive embryonic research, such as the one sponsored by Senate President Don Hines (D-Bunkie), would not receive her support. (lifenews.com)

Dr. George Daley and correspondent Robert Bazell discuss the significance and ethics of embryonic stem cell cloning. (charlierose.com)

Mouse embryonic stem cells are known to be tumorigenic - that is, they can grow uncontrollably and become cancerous. (spiked-online.com)
BayGenomics: a resource of insertional mutations in mouse embryonic stem cells. (genscript.com)

A House Democrat has filed an amendment to the state budget that would bar the use of Department of Corrections funds for abortion, cloning or stem cell research. (missourinet.com)
The House has given first round approval to legislation meant to allow medical workers in Missouri refuse to participate in certain procedures including abortion, human cloning and stem cell research if they have a religious or moral objection to them. (missourinet.com)
Public funding of abortion or embryonic stem cell research. (iowacatholicconference.org)
Do you exclude advocates of abortion, euthanasia, embryonic stem-cell research or cloning as commencement speakers or recipients of honorary degrees? (thomasaquinas.edu)

A clone is an organism that is a genetic copy of an existing one. (who.int)
At that point - and this is important to understand - there is no more cloning to be done since a new human organism now exists. (cbc-network.org)
If the authors of this bill really meant what they appear to have written, their legislation would ban all human cloning, since as we have seen, biologically, a new human organism, that is, a new human being, comes into existence with the completion of SCNT. (cbc-network.org)
Or to put it the other way around, cloning, not implantation, is what produces a new and distinct human organism. (cbc-network.org)
The science fiction definition of "clone" suggests that the cloned organism would be an exact genetic copy of another creature-human or beast-created in the laboratory by any of a number of means. (all.org)
There are differences-so much so that despite the "exact copy" claim, the cloned organism is actually unique genetically. (all.org)

His work has led to major advances in our understanding of mammalian cloning and embryonic stem cells. (wraltechwire.com)

He is the co-author of the book The Natural Limits to Biological Change , served as general editor of Creation, Evolution and Modern Science , co-author of Basic Questions on Genetics, Stem Cell Research and Cloning (The BioBasics Series) , and has published numerous journal articles. (probe.org)

And the other technique, which produces " induced pluripotent stem cells ," skips the step that requires a human egg cell, so some people find it less fraught, ethically. (usf.edu)
An essential function of SOX2 is to stabilize embryonic stem cells in a pluripotent state by maintaining the requisite level of Oct 3/4 expression. (thermofisher.com)
Induced pluripotent stem cells, another types of pluripotent stem cells derived from any tissue by reprogramming and are the homologous source of stem cells. (benthamscience.com)
The TRA-1-60 monoclonal antibody reacts with the neuraminidase-resistant form of a pluripotent-stem-cell-specific epitope on a high-molecular-weight transmembrane glycoprotein. (bdbiosciences.com)
Right now, the hottest area in stem cell biology is that of induced pluripotent stem cells, or iPS cells, which have the ability to develop into several different tissue types. (cbc-network.org)
Up to 14 days a human blastocyst - the earliest stage of fetal development - consists almost entirely of pluripotent cells, which are those that could develop into the constitutive elements of any organ in the human body. (thetablet.org)
In reality, gene somatic cells to a pluripotent cell state by a handful of transcrip- expression is graded, making the potential gene expression tion factors (Takahashi and Yamanaka, 2006). (lu.se)

Dolly, the first mammal to be genetically cloned from adult cells, poses for the camera in 1997 at the Roslin Institute in Edinburgh, Scotland. (usf.edu)
WHA50.37 of 1997 argues that human cloning is ethically unacceptable and contrary to human integrity and morality. (who.int)
He stayed on to earn his Ph.D. in molecular biology at Cambridge, training under the legendary geneticist John Gurdon, whose breakthroughs in the 1950s and 1960s were key to the experiments performed by Ian Wilmut, a Gurdon student who cloned Dolly the sheep in 1997. (discovermagazine.com)
Note: Please read The Little Lamb That Made a Monkey of Us All for the author's comments on the news of a successful lamb cloning (March 7, 1997). (probe.org)

For pediatric patients with refractory cytopenia, certain cytogenetic abnormalities, or malignant transformation, hematopoietic stem cell transplantation (HSCT) from a matched related or unrelated donor early in the course of the disease is the treatment of choice. (medscape.com)
MDS is rare in childhood and may have a rapidly progressive course with an extremely poor prognosis without hematopoietic stem cell transplantation (HSCT). (medscape.com)

This Study presents the first use in somatic cell nuclear transfer (SCNT) of tooth Pulp cells containing progenitor cells that resemble the nonaging embryonic connective tissue in adults. (yildiz.edu.tr)

Mouse nuclear transfer embryonic stem cells (NT-ESCs) were first established in 2000, and then proved to be able to differentiate either in vivo or in vitro, and give rise to individual tissues through germ line transmission or tetraploid complementation. (benthamscience.com)
The primary cloning technique is called "somatic cell nuclear transfer" (SCNT). (cbc-network.org)
Here, the stem cell line is created using the genetic properties of the prospective recipient via somatic cell nuclear transfer. (spiked-online.com)
More than 100 nuclear transfer procedures could be required to produce one viable clone. (wikiquote.org)

Using tooth Pulp Cells ill SCNT increased the first cleavage rates compared with adult somatic Cells, leading to a higher rate of DNA reprogramming and increased production of an identical embryonic stem cells (ESCs) line. (yildiz.edu.tr)
These cells were named Osmangazi Turk Identical ESCs since this was the first use in SCNT of tooth pulp cells to demonstrate a decrease in glucose levels following administration of these cells. (yildiz.edu.tr)
This is junk biology since implanting isn't the act of asexual reproduction: SCNT cloning is. (cbc-network.org)

Human embryonal carcinoma (EC) cells are the stem cells of teratocarcinomas, and they are key components of germ cell tumors (GCTs). (sigmaaldrich.com)
The antigen is found on teratocarcinoma (embryonal carcinoma or EC), embryonic inner cell mass (but not morula or trophoblast), and embryonic stem (ES) cells. (bdbiosciences.com)

Finally, the cloners see that there is no political stability for their clone and kill labs in Louisiana, meaning that effective adult stem cell research can continue to thrive in Louisiana," concluded Bordlee. (lifenews.com)
Keep in mind, this is ethical adult stem cell research, which is in human clinical trials, helping patients NOW. (cbc-network.org)

ESCs derived from tooth Pulp cells using this method were observed to transfer into pancreatic beta Cells using transmission electron microscopy. (yildiz.edu.tr)
What is more, by deriving NT-ESCs from patient cells, the problem of immune rejection may be avoided. (benthamscience.com)

It is the policy of Washington state that research involving the derivation and use of human embryonic stem cells, human embryonic germ cells, and human adult stem cells from any source, including somatic cell nuclear transplantation , is permitted upon full consideration of the ethical and medical implications of this research. (cbc-network.org)

In the 18 years since researchers cloned a sheep, scientists have found another way to produce cloned human cell lines. (usf.edu)
Two other independent researchers, Severino Antinori (an Italian working in an undisclosed Muslim country) and Panos Zavos (from Lexington, Kentucky) have also been hinting at human cloning success and suggesting that a birth will be announced soon. (probe.org)
This incredibly high 50% success rate for human cloning leaves most researchers believing that either this isn't really a clone or they simply aren't revealing all the other failures. (probe.org)
Researchers from the Harvard Stem Cell Institute have identified a vital protein that can help determine embryonic development. (scitechdaily.com)
A protein that is necessary for the formation of the vertebrate brain has been identified by researchers at the Harvard Stem Cell Institute (HSCI) and Boston Children's Hospital, in collaboration with scientists from Oxford and Rio de Janeiro. (scitechdaily.com)
These findings could benefit stem cell researchers trying to create specific tissue types or organs in the lab. (scitechdaily.com)
In order to guide or direct stem cells to differentiate into a given cell type, such as neural cells or muscle cells, researchers continue to alter their experimental recipes, fine-tuning which molecules should be added to their dishes in what sequence and amount. (scitechdaily.com)
For those trying to create neural cells, "Notum is a necessary ingredient and new tool in the kit box for researchers to instruct human progenitor cells to become neural tissues," said He, who is also an American Cancer Society research professor. (scitechdaily.com)
Additionally, the researchers were able to demonstrate how Notum deactivates Wnt, which is a family of proteins that direct stem cells to "self-renew," or make more stem cells, among other things. (scitechdaily.com)
Researchers are making great strides with hair cloning, but I have no idea how many years it's going to be before anything reliably safe and effective is commercially available. (baldingblog.com)
Recently, researchers from the Sanger Institute (UK) have isolated stable and germline competent embryonic stem (ES) cells from C57BL/6N mice (1). (umassmed.edu)
Below you can see some examples of the infrastructure for research on genes and cells, available for researchers at Lund University. (lu.se)

SOX2 is an intronless gene encoding a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. (thermofisher.com)

These Outcomes are promising for regenerative medicine ill the therapy of diabetes mellitus, and clarification of the importance of selecting appropriate adult somatic cells in deriving identical ESC lines is also a significant step. (yildiz.edu.tr)

Specificity Comments: SOX2 is required for stem cell maintenance in the central nervous system, and it also regulates gene expression in the stomach. (thermofisher.com)
Resolution of cell fate decisions revealed by single-cell gene expression analysis from zygote to blastocyst. (nature.com)
Expression of TRA-1-60 antigen has also been observed on a rhesus monkey ES cell line (Thomson et al, 1995). (bdbiosciences.com)
ORF sequences can be delivered in our standard vector, pcDNA3.1 + /C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. (genscript.com)
Knowledge on the expression analysis of Notch signaling pathway molecules in SHED cultured in KGM could highlight its involvement in controlling the biological activity of these stem cells, particularly during odontogenesis and other developmental process. (bvsalud.org)
In this study, we showed that a one-hour treatment with topoisomerase inhibitors (camptothecin and etoposide) prior to viral transduction is enough to increase AAV and LV reporter expression in non-dividing hepatic cells in culture. (bvsalud.org)
Similarly, GATA-1 has been shown to induce lineage switching expression values even if, for simplicity, we assume only ``on'' of committed cells in hematopoiesis, first in cell lines (Kulessa and ``off'' states for each gene. (lu.se)

Dolly the sheep was cloned in 1996. (thetablet.org)

WHA50.37, which states "the use of cloning for the replication of human individuals is ethically unacceptable and contrary to human integrity and morality. (who.int)
While stem-cell research holds enormous potential for treating or even curing some diseases, the cloning of a human being is morally and ethically unacceptable…Any attempt to clone a human being is in direct conflict with the public policies of this state. (cbc-network.org)

from nationalreview.com Let's call it "stealth human-cloning legalization. (cbc-network.org)

He'd like to see a library of cells created with those carefully chosen genes. (usf.edu)
Alternatively, transgenesis and gene targeting techniques can be used to introduce the patient's genes into the stem cell line. (spiked-online.com)
explosion further, consider that a fictitious small genome with 2002) More recently and more dramatically, the potential for 260 genes would host the same number of combinations as cell state conversions is exemplified by the reprogramming of the number of atoms in the visible universe! (lu.se)
Below is a non-exhaustive list of in-house infrastructures that are categorized into three overarching themes: bio-imaging, proteins, genes & cells and other resources. (lu.se)
In addition to infrastructures for bioimaging, protein and genes & cells, we also provide other resources e.g., databases, networks and specialized labs. (lu.se)

This antibody reacts with TRA-1-60 antigen that is expressed upon the surface of human tetracarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). (sigmaaldrich.com)
The TRA-1-60 antigen is a sialylated epitope on the same keratan sulfate core molecule, podocalyxin, as 4 other distinct antigens on tumor-derived cell lines, TRA-1-81, GCTM2, K4, and K21. (bdbiosciences.com)
TRA-1-60 antigen is released into the serum of patients bearing testicular tumors containing EC cells. (bdbiosciences.com)
The stage-specific embryonic antigen 4 (SSEA4) is com- isolate the NSCs from neonatal mice and rats (Campos monly used as a cell surface marker to identify the pluri- et al. (lu.se)
The stage-specific embryonic antigen 4 (SSEA4) is brain. (lu.se)

Current screening of potential new drugs is done using cell lines derived from animals or 'abnormal' human tissue such as tumor cells. (spiked-online.com)
If there are intact cells in this tissue they have been 'stored' frozen. (wikiquote.org)
A blastocyst (cloned or not), because it lacks any trace of a nervous system, has no capacity for suffering or conscious experience in any form - the special properties that, in our view, spell the difference between biological tissue and a human life worthy of respect and rights. (wikiquote.org)
Obtaining stem cells from fatty tissue, bone marrow, or the umbilical cord after the birth of a baby, on the other hand, may be done ethically. (all.org)
Recent news of an impending clinical cell transplantation trial in Parkinson's disease using parthenogenetic stem cells as a source of donor tissue have raised hopes in the patient community and sparked discussion in the research community. (lu.se)

The protein, Notum, first discovered in fruit flies in 2002 and then found in mice and humans, is one of many that help determine embryonic development. (scitechdaily.com)
Our facilities provide the opportunity to study protein structure, molecular probes and drug design, system biology and molecular interactions in cells and tissues. (lu.se)

The resulting egg was implanted in the womb of a third sheep, and the result was Dolly, the first clone of a mammal. (usf.edu)

Meanwhile, the ability of the mature egg to transform and begin embryonic development remains fully potent. (cbc-network.org)
2005). Notch1 and syndecan-1 potent human embryonic stem (ES) cells. (lu.se)

Margaret Somerville, founding director of the McGill Centre for Medicine, Ethics and Law at McGill University in Montreal, makes her case from a purely academic and secular perspective in a comment published in the National Post last week, called "The ethics of stem cells. (catholicnewsagency.com)
Research ethics and lessons from Hwanggate: what can we learn from the Korean cloning fraud? (bmj.com)
Great Iranian Muslim scholars netics, stem cell research, and organ trans- laid huge emphasis on teaching and practis- plantation are some of the medical issues ing ethics. (who.int)

Here, we characterize SpCas9 targeting specificity in human cells to inform the selection of target sites and avoid off-target effects. (cdc.gov)

The study of biology of stem cells is the hallmark of the recent emerging field of regenerative medicine and medical biotechnology. (benthamscience.com)
In biology , cloning is the process of producing similar populations of genetically identical individuals that occurs in nature when organisms such as bacteria , insects or plants reproduce asexually . (wikiquote.org)
Understanding cell-fate decisions in stem cell populations is a major goal of modern biology. (lu.se)

Scientists anticipate that in the future stem cell lines will provide a virtually unending supply of pancreatic cells for diabetic patients, neuronal cells for patients with neural disorders such as Parkinson's or Alzheimer's disease, and a host of heart cells that may treat a variety of cardiac problems. (spiked-online.com)
CD133+), but are rarely codetected with the neural stem dents, very few human-specific NSC markers have been cell (NSC) marker CD15. (lu.se)

Furthermore, 129-strain ES cells often contribute to the germline of the chimeras, and when breeding chimeras with C57BL/6 test mice, the dominant agouti color in offspring signifying germline transmission is also easily detected in the G1 generation litters. (umassmed.edu)
These ES cells allow for the generation of gene‐targeted mice on a C57BL/6 genetic background without the need for multiple backcrossing of mice. (umassmed.edu)
To generate suitable C57BL/6 embryonic stem cells for gene targeting experiments, the Sanger team established a male cell line (JM8) from the N-substrain of C57BL/6 mice. (umassmed.edu)
When these C57BL/6N-based JM8 cells were injected into blastocysts from albino mice, a high proportion of chimeras displayed ES cell contribution to both germline and somatic tissues. (umassmed.edu)
The Sanger group performed gene targeting in the JM8 ES cells to delete the retrotransposon from the agouti locus and restore agouti gene function, permitting the visualization of ES cell-derived mice on an inbred C57BL/6 background by agouti coat color. (umassmed.edu)
Since these JM8A3 cells are heterozygous for the corrected agouti allele [A tm1brd ], crossing the resulting brown-on-white chimeras with C57BL/6N test mice yield embryonic stem cell-derived offspring with either agouti or black coats. (umassmed.edu)
However, one still has to genotype all of the G1 generation mice from germline transmitting chimeras or risk losing some black colored mice that are ES cells derived. (umassmed.edu)
Immunohisto- have been used for positive selection of NSCs from em- chemistry on human embryonic central nervous system bryonic mice (Nagato et al. (lu.se)

Developments in biotechnology have raised new concerns about animal welfare, as farm animals now have their genomes modified (genetically engineered) or copied (cloned) to propagate certain traits useful to agribusiness, such as meat yield or feed conversion. (wikiquote.org)

And yet, all of us would be appalled at the idea of terminating their lives so we could harvest their tissues or organs in order to save others," she says, in reference to the common utilitarian argument that embryonic stem-cell research is valid in an effort to find cures that could save people's lives. (catholicnewsagency.com)
Blastocytes obtained through nuclear transfers would be used to generate the embryonic stem cells that could be differentiated to specific tissues or organs for transfer to the nuclear donor. (spiked-online.com)
Stem cells from human exfoliated deciduous teeth (SHED) are multipotent stem cells derived from the pulp tissues of extracted deciduous teeth 1 . (bvsalud.org)

A new controversy is brewing over government backing of Ebola vaccines that are using aborted fetal cell lines even though vaccines developed from moral alternatives are just as effective. (womenofgrace.com)
1983) and the multipotent progenitor cells from fetal disease (Bjorklund and Lindvall, 2000). (lu.se)

The "stem cell theory of cancer" states that a subpopulation of cells with stem cell-like properties plays a central role in the formation, sustainment, spread, and drug resistant characteristics of malignant tumors. (medscape.com)
Recent studies have isolated distinct cell populations from infantile hemangiomas that display properties equivalent to aberrant progenitor cells, suggesting that, in addition to malignant tumors, benign tumors may also contain a stem cell-like component. (medscape.com)
These findings provide supporting evidence that enrichment for proteins involved in pluripotency is not restricted solely to malignant tumors as is suggested by the "stem cell theory of cancer", but additionally extends to common benign vascular tumors such as hemangiomas. (medscape.com)
Resolving the Pathogenesis of Anaplastic Wilms Tumors through Spatial Mapping of Cancer Cell Evolution. (lu.se)

Cloning in biotechnology refers to processes used to create copies of DNA fragments ( molecular cloning ), cells (cell cloning), or organisms . (wikiquote.org)

Scientists at Stanford University in California have been able to coax embryonic stem cells into becoming eggs and sperm, which could one day lead to the creation of children through entirely artificial means. (womenofgrace.com)
Hair growth in adults occurs naturally in a process known as hair neogenesis â€" where cells called dermal papilla cells that span the top two layers of skin coax surrounding cells to form hair follicles. (baldingblog.com)

Eighteen years ago, scientists in Scotland took the nuclear DNA from the cell of an adult sheep and put it into another sheep's egg cell that had been emptied of its own nucleus. (usf.edu)
So ideally scientists would like to be able to extract DNA from the cells of older people - not just cells from infants - to create therapies for adult diseases. (usf.edu)
In principle, scientists could produce a series of cell lines that would allow a close match for the majority of would-be cell recipients - just as transplant surgeons currently seek a close match for organ donors. (usf.edu)
British scientists are hailing the recent creation of human sperm cells that they believe could revolutionize fertility treatment. (womenofgrace.com)
1. Cloning is an umbrella term traditionally used by scientists to describe different processes for duplicating biological material. (who.int)
Scientists, many of whom are sold on utilitarian-based ethical analysis, try to downplay the issue of human life in stem-cell research. (catholicnewsagency.com)
As part of Charlie's ongoing Science Series, co-host Sir Paul Nurse and a panel of scientists tackle the controversial subject of stem cell research. (charlierose.com)
It might be expected that the richest nation on Earth would encourage its top scientists to pursue this work with vigor rather than limiting funding opportunities, creating legal barriers and fencing off any newly developed cell lines. (spiked-online.com)
"PrimeGen , based in Irvine, California, says that its scientists have converted specialised adult human cells back to a seemingly embryonic state - using methods that are much less likely to trigger cancer than those deployed previously. (cbc-network.org)
The company also claims to be able to produce reprogrammed cells faster and much more efficiently than other scientists. (cbc-network.org)

The U.S. Food and Drug Administration (FDA) has approved the world's first human clinical trial of a therapy involving embryonic stem cells, a move that has been condemned by the Vatican as "unacceptable. (womenofgrace.com)
The non-embryonic stem cells like adult stem cells are in clinical use for many years and embryonic stem cells are now emerging as an alternative source for the same purpose with huge potentials in drug discovery and toxicological studies. (benthamscience.com)
There are major obstacles to overcome before a stem cell line is liable to reach clinical trials. (spiked-online.com)
We will also explore whether lower MIH will be related to higher cell-free mtDNA (ccf-mtDNA) levels and poor clinical outcomes. (bvsalud.org)
The biological properties and clinical potential of stem cells elicit that are generated must not be unduly sensitive to small fluctu- continued scientific, commercial, and public interest. (lu.se)
Based on discussions held by a global collaborative initiative on translation of stem cell therapy in Parkinson's disease, we have identified a set of key questions that we believe should be addressed ahead of every clinical stem cell-based transplantation trial in this disorder. (lu.se)
Stem cell-based therapies for Parkinson's dis- ogy company International Stem Cell Corporation ease (PD) are rapidly moving towards clinical trials. (lu.se)
A second press release [2] has indicated stem cell source, resulting in widespread excitement that the program is planning to move forward very rapidly, with all of the patients being enrolled in the Correspondence to: Roger A. Barker, John van Geest Centre first quarter of 2016 and interim results being shared for Brain Repair, Department of Clinical Neurosciences, Univer- in October 2016. (lu.se)

The following product was used in this experiment: SOX2 (Embryonic Stem Cell Marker) Recombinant Rabbit Monoclonal Antibody (SOX2/4267R) from Thermo Fisher Scientific, catalog # 6657-RBM7-P1. (thermofisher.com)
However, SOX2 is expressed in a high percentage of lung squamous cell carcinomas and has been shown to be an independent favorable prognostic marker. (thermofisher.com)
RNA was reverse-transcribed to obtain the cDNA and then proceeded with PCR using specific primers for the Notch signaling pathway molecules (Notch1, Jagged-1, Jagged-2 and, Hes1) as well as stem cell marker (Nanog). (bvsalud.org)

It also means that finally getting the sheep technology to work with cells from adult humans may not turn out to be a turning point for this technology, after all. (usf.edu)
Can Humans Be Cloned Like Sheep? (probe.org)
The intent of this legislation is to prevent the cloning of humans,' said Rep. Beard. (lifenews.com)
2002). In humans, SSEA4 is expressed by building the nervous system but also for their prospec- nonneural cells such as the erythrocytes (Kannagi et al. (lu.se)

Peripheral blood mononuclear cells (PBMCs) were used to measure the enzymatic activities of citrate synthase and complexes I, II, and IV and mtDNA copy number. (bvsalud.org)

Rossant, J. & Tam, P. P. L. Blastocyst lineage formation, early embryonic asymmetries and axis patterning in the mouse. (nature.com)
Fleming, T. P. A quantitative analysis of cell allocation to trophectoderm and inner cell mass in the mouse blastocyst. (nature.com)

During a recent appearance on the Oprah Winfrey show, the popular cardiovascular surgeon and television personality, Dr. Mehmet Oz, announced to a stunned audience that due to advances in adult stem cell technology, the debate over embryonic stem cells "is dead. (womenofgrace.com)
Dr. Gerald Fischbach, dean of Columbia Medical School, and others explain advances in stem cell research. (charlierose.com)
He said cloning research was no longer necessary because of recent advances in stem cell science. (bioedge.org)

In neu- fatal neurologic disease of horses and sheep, owes rons, sometimes in glia cells, acidophilic intra- its name to the town Borna in Saxony, Germany, nuclear inclusion bodies, called Joest-Degen inclu- where a large number of horses died during an sion bodies, are occasionally found. (cdc.gov)

The Vatican document "Dignitas Personae" ("The Dignity of a Person") warns that certain recent developments in stem-cell research, gene therapy and embryonic experimentation violate moral principles and reflect an attempt by man to "take the place of his Creator. (thetablet.org)

Although low ROS levels are beneficial to cellular stress responses for the activation of several cellular signaling pathways, abnormally elevated ROS leads to damage to cells and organs, and eventually, to cell death, thereby ROS can be either beneficial or detrimental to health 11 . (nature.com)
Beyond 14 days the fetus becomes more complex and cells begin to acquire the specific attributes of the organs they will become. (thetablet.org)
Our facilities provide the opportunity to study molecules, cells, organs and entire organisms. (lu.se)

Dolly's birth set off a huge outpouring of ethical concern - along with hope that the same techniques, applied to human cells, could be used to treat myriad diseases. (usf.edu)
The director of the lobby group Australians for Ethical Stem Cell Research, David van Gend, criticised the issuing of the licence. (bioedge.org)

Johnson, M. H. & Ziomek, C. A. The foundation of two distinct cell lineages within the mouse morula. (nature.com)

H9 human embryonic stem (ES) cells were harvested with Accutase™ and stained with either PE Mouse Anti-Human TRA-1-60 (solid line) or PE mouse IgM (G155-228) isotype control (Cat. (bdbiosciences.com)

And with a bit of coaxing, these cells could, theoretically, be prodded to turn into any sort of human cell - nerve, heart, liver and pancreas, for example. (usf.edu)

For Melton's children, this imbalance is most likely an early death sentence, unless, of course, their father can discover a stem cell fix for pancreatic cells in diabetics that are inexplicably attacked by the body's immune system. (discovermagazine.com)

Anatomy52

Organisms12

Diseases7

Chemicals and Drugs65

Analytical, Diagnostic and Therapeutic Techniques and Equipment59

Phenomena and Processes63

Disciplines and Occupations4

Technology, Industry, Agriculture1

Information Science3

Embryos17

Therapeutic18

Differentiation15

Embryo19

Reproductive7

Correspondent Robert Bazell discuss the significance1

Mouse embryonic2

Abortion4

Organism6

Mammalian1

Research and Cloning1

Pluripotent7

19974

Hematopoietic Stem Cell Trans2

Connective tissue1

Nuclear transfer4

SCNT3

Embryonal carcinoma2

Adult stem cell res2

ESCs2

Derivation1

Researchers12

Involved in the regulation1

Somatic cells1

Expression7

19961

Ethically unacceptable2

Legalization1

Genes5

Antigen5

Tissue5

Protein2

Dolly1

Potent2

Ethics3

Characterize1

Biology3

Neural2

Mice8

Genetically engineered1

Tissues3

Fetal2

Tumors4

Biotechnology1

Coax2

Scientists10

Clinical8

Marker3

Humans4

Mononuclear cells1

Blastocyst2

Advances3

Sheep1

Moral1

Organs3

Ethical2

Morula1

Isotype1

Human cell1

Pancreatic1