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  • morphology
  • These hESC-derived adipocyte precursor cells display a characteristic morphology and can be propagated and cryopreserved as a standard stem cell line. (edu.au)
  • After two weeks of spontaneous differentiation under feeder-free conditions, 60-70% of cells display a mature adipocyte morphology as well as the expression of multiple adipocyte-specific mRNAs as assessed by RT-PCR. (edu.au)
  • fetal
  • Additionally, microarray analysis of isolated ES cell-derived HSPCs demonstrated differences in the gene expression from native HSCs isolated from the fetal liver or bone marrow of mice. (utexas.edu)
  • A first wave of hematopoiesis follows MHE colony emergence and is predominated by primitive erythropoiesis characterized by a brilliant red hemoglobinization, CD71/CD325a (glycophorin A) expression, and exclusively embryonic/fetal hemoglobin expression. (bloodjournal.org)
  • poorly understood
  • However, the mechanisms by which ES cells are committed to the neural lineage using this technique are poorly understood. (biologists.org)
  • However, the detailed functions and the mechanism by which Rictor/mTORC2 may affect embryonic cardiogenesis remains poorly understood. (ijbs.com)
  • genes
  • Lentiviral silencing of FoxO1 in pancreatic progenitors upregulated the levels of pancreatic islet differentiation-related genes and improved glucose-stimulated insulin secretion response in their progeny IPCs, whereas overexpression of FoxO1 showed the opposite effects. (ovid.com)
  • From left: Zhong Liu, Ph.D., Rui Zhao, Ph.D., and Xiaosi "Stan" Han, M.D., Ph.D. The more than 200 different types of human cells have the same DNA but express different ensembles of genes. (uab.edu)
  • However, it is not clear if single genes can define a cell̀s identity and if the cell fate defining potential of TFs is also operative in pluripotent stem cells in vitro. (sigmaaldrich.com)
  • These findings demonstrate that a single gene is sufficient to determine cell fate decisions of uncommitted stem cells thus giving insights into the role of key developmental genes during lineage commitment. (sigmaaldrich.com)
  • progenitors
  • Bouhon IA, Joannides A, Kato H, Chandran S, Allen ND (2006) Embryonic stem cell-derived neural progenitors display temporal restriction to neural patterning. (springer.com)
  • In addition, rotary (Synthecon) bioreactors produced more sca-1⁺ progenitors, and spinner flasks generated more c-kit⁺ progenitors, demonstrating their unique differentiation profiles. (utexas.edu)
  • A second wave of definitive-type erythroid burst-forming units (BFU-e's), erythroid colony-forming units (CFU-e's), granulocyte-macrophage colony-forming cells (GM-CFCs), and multilineage CFCs follows next from hEB progenitors. (bloodjournal.org)
  • efficiency
  • Therefore, new tools need to be developed in order to improve the efficiency of stem cell differentiation towards specific cardiovascular lineages. (mdpi.com)
  • Gerecht-Nir S, Cohen S, Itskovitz-Eldor J (2004) Bioreactor cultivation enhances the efficiency of human embryoid body (hEB) formation and differentiation. (springer.com)
  • Insight gained from this work should be continued by comparing the differentiation efficiency of HSPCs derived in dynamic and traditional static culture methods into functional, terminally differentiated hematopoietic cells to generate clinically-relevant numbers of transplantable, therapeutic cells. (utexas.edu)
  • fibroblast
  • With genetic and biochemical tricks, researchers can reverse a differentiated cell - such as a skin fibroblast - into a pluripotent state. (uab.edu)
  • H1 [Wi-Cell] and hES-NCL1 [Newcastle University, United Kingdom] hES cells were then plated on the collagen IV using limbal fibroblast conditioned medium. (arvojournals.org)
  • ventricular
  • Knockdown of Rictor resulted in inhibiting ventricular-like myocytes differentiation and induced arrhythmias symptom, which was accompanied by interfering the expression and distribution patterns of cell-cell junction proteins. (ijbs.com)
  • induction
  • Approximately 50% of these phosphosites were regulated within 1 hr of differentiation induction, revealing a complex interplay of phosphorylation networks spanning different signaling pathways and kinase activities. (nih.gov)
  • Although ES cells are clonally established, they display heterogeneous responses upon the induction of differentiation, resulting in a mixture of various types of differentiated cells. (mdpi.com)
  • embryo
  • The microenvironment surrounding the developing embryo presents these instructive cues in a complex and interactive manner in order to guide cell fate decisions. (semanticscholar.org)