AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesDisciplines and OccupationsTechnology, Industry, AgricultureInformation Science
EndodermEmbryonic Stem CellsNodal Signaling LigandsSOX Transcription FactorsEmbryonic InductionStem CellsCell DifferentiationSOXF Transcription FactorsPluripotent Stem CellsHepatocyte Nuclear Factor 3-betaEmbryo, MammalianNodal ProteinGene Expression Regulation, DevelopmentalCell LineageActivinsGerm LayersStem Cell TransplantationEmbryoid BodiesMesodermCell Culture TechniquesOctamer Transcription Factor-3Primitive StreakHomeodomain ProteinsHematopoietic Stem CellsInduced Pluripotent Stem CellsHMGB ProteinsCell LineGastrulaCells, CulturedAdult Stem CellsTranscription FactorsFibroblast Growth Factor 4Signal TransductionDigestive SystemTeratomaSOXB1 Transcription FactorsNeural Stem CellsBody PatterningEmbryo ResearchIn Situ HybridizationGene Expression ProfilingReverse Transcriptase Polymerase Chain ReactionMultipotent Stem CellsEmbryonic DevelopmentGastrulationMorphogenesisWnt3 ProteinStem Cell NicheHematopoietic Stem Cell TransplantationCell ProliferationEmbryonic and Fetal DevelopmentDNA-Binding ProteinsChimeraTotipotent Stem CellsFlow CytometryBlastocystMice, TransgenicBiological MarkersGene ExpressionGene TargetingMesenchymal Stromal CellsLeukemia Inhibitory FactorMesenchymal Stem Cell TransplantationCulture Media, Serum-FreeBone Morphogenetic Protein 4Gene Expression RegulationStem Cell ResearchMice, Inbred C57BLTrans-ActivatorsProsencephalonPancreasEctodermRNA, MessengerEnzyme InductionStem Cell FactorStage-Specific Embryonic AntigensGATA6 Transcription FactorGreen Fluorescent ProteinsFeeder CellsHematopoiesisNuclear ReprogrammingHorseradish PeroxidaseHepatocytesCell SeparationBase SequenceCoculture TechniquesYolk SacGerm CellsTretinoinTime FactorsRegenerative MedicineImmunohistochemistryNeuronsMolecular Sequence DataPhenotypeTransforming Growth Factor betaMice, KnockoutNuclear ProteinsModels, BiologicalMyocytes, Cardiac
PluripotentMesodermESCsVitroEctodermExtra-embryonic tissuesGastrulationTissuesLineagesEmbryosSox17PancreaticDifferentiateGermEpitheliumPluripotencySpecificationDevelopmentalNeuralPSCsTranscriptionalBiologyDevelopmentProgenitorMicroenvironmentVisceralFormationGenesEndodermalMolecularMammalianMouseFoxa2RegulatorPrecursorsEpithelial cellMesenchymal cellsGenerateAntibodiesAdherentInduceSomatic cellsPopulationsPosteriorGene expressionHPSCsMetabolismIntracellularFibroblasts
Pluripotent26
Mesoderm6
  • During gastrulation anterior endoderm (AE) provides cardiogenic signals that act on adjacent mesoderm , resulting in induction of cardiac precursors. (xenbase.org)
  • Such human fully integrated and complete SEMs recapitulate the organization of nearly all known lineages and compartments of post-implantation human embryos, including the epiblast, the hypoblast, the extra-embryonic mesoderm and the trophoblast layer surrounding the latter compartments. (nature.com)
  • These include embryonic disc and bilaminar disc formation, epiblast lumenogenesis, polarized amniogenesis, anterior-posterior symmetry breaking, primordial germ-cell specification, polarized yolk sac with visceral and parietal endoderm formation, extra-embryonic mesoderm expansion that defines a chorionic cavity and a connecting stalk, and a trophoblast-surrounding compartment demonstrating syncytium and lacunae formation. (nature.com)
  • The fertilized oocyte is totipotent, with resultant blastomeres capable of generating all three primary germ layers, ectoderm, mesoderm, and endoderm, in addition to the extra embryonic tissues. (conditionmed.org)
  • In addition, even in the absence of exogenous supplements, ectoderm, mesoderm, endoderm, and extraembryonic cells differentiated following the induction of collective cell migration at the colony edge by removing the ring-barrier. (biomedcentral.com)
  • Our findings suggest that collective cell migration can be an effective way to drive the segregation of mesoderm and endoderm lineages, and cell fate decisions of hiPSCs. (biomedcentral.com)
ESCs2
  • Here, we present a quantitative and comprehensive study of the abundance levels of histone PTMs during the differentiation of mouse embryonic stem cells (ESCs) using mass spectrometry (MS). We observed dynamic changes of histone PTMs including increased H3K9 methylation levels in agreement with previously reported results. (biomedcentral.com)
  • Dorsomorphin dihydrochloride also inhibits bone morphogenetic protein (BMP) type I receptors (ALK2, ALK3 and ALK6), promotes cardiomyogenesis in mouse embryonic stem cells (ESCs) in vitro and promotes neural differentiation of hPSCs as part of a chemical cocktail. (tocris.com)
Vitro10
  • Gene therapy has been utilized successfully to repair or inactivate mutations in animal models of monogenic human diseases 19 as well as human in vitro cells 12 . (nature.com)
  • These findings reveal a set of cellular changes that may represent the earliest markers of in vitro hESC transition to an epithelial phenotype, before the induction of gene expression networks that guide hESC differentiation. (northwestern.edu)
  • Un rôle majeur des prostaglandines dans l'hématopoïèse, ainsi mis en évidence, a pu être étendu à la souris chez qui un traitement par la prostaglandine PGE2 provoque la multiplication des CSH in vivo et in vitro, un résultat qui génerera très probablement des applications cliniques. (hypotheses.org)
  • We discuss what is known about the distinct metabolic states captured in vitro by the 2-cell-like, naïve, blastocyst-like, formative, and primed states of pluripotency. (conditionmed.org)
  • 2019). Akin to the dynamic nutrient requirements of the developing embryo, discrete in vitro cell states have distinct metabolic profiles (Zhou et al. (conditionmed.org)
  • It is only through understanding embryonic metabolism and development that we can derive and maintain different in vitro stem cell states for disease modeling and therapies. (conditionmed.org)
  • On the other hand, even if 3D aggregate culture methods are able to recapitulate in vitro the cell-extracellular matrix interactions properly observed in vivo, and the synthetic/natural matrix and scaffolds have biochemical and mechanical properties, in order to mimic the native extracellular matrix, both of these systems possesses some limitations and some methodological improvements are needed. (razavihospital.ir)
  • Results: The processes by which re-aggregated adult single cell types or adult and embryonic explanted tissues are able to recapitulate embryogenesis in vitro, when cultured in adhesion or embedded in 3D gels, is not surprising and is clearly under the control of a reminiscent cellular memory which recapitulates early developmental stages. (razavihospital.ir)
  • Moscona A. The Development in Vitro of Chimeric Aggregates of Dissociated Embryonic Chick and Mouse Cells. (razavihospital.ir)
  • Kurosawa H. Methods for inducing embryoid body formation: in vitro differentiation system of embryonic stem cells. (razavihospital.ir)
Ectoderm1
  • Epithelia (ectoderm and endoderm) form the majority of the "ductless" endocrine glands like gastrointestinal and skin associated "ducted" glands. (edu.au)
Extra-embryonic tissues2
  • Embryo-like models with spatially organized morphogenesis and structure of all defining embryonic and extra-embryonic tissues of the post-implantation human conceptus (that is, the embryonic disc, the bilaminar disc, the yolk sac, the chorionic sac and the surrounding trophoblast layer) remain lacking 1 , 2 . (nature.com)
  • Much of this process relies on the morphogenesis of the extra-embryonic tissues and the effect this has on the organization of embryonic cells. (nature.com)
Gastrulation4
  • Mouse naive embryonic stem cells have recently been shown to give rise to embryonic and extra-embryonic stem cells capable of self-assembling into post-gastrulation structured stem-cell-based embryo models with spatially organized morphogenesis (called SEMs) 3 . (nature.com)
  • Embryo-like structures generated from stem cells can achieve varying developmental milestones, but none have been shown to progress through gastrulation, neurulation, and organogenesis. (stemjnl.org)
  • 1-7 Here, we show that "ETiX" mouse embryos, established from embryonic stem cells aggregated with trophoblast stem cells and inducible extraembryonic endoderm stem cells, can develop through gastrulation and beyond to undertake neural induction and generate the progenitors needed to create the entire organism. (stemjnl.org)
  • Collective cell migration modulates self-organized fate patterning decisions in iPSC-derived gastrulation-stage meso-endoderm. (biomedcentral.com)
Tissues4
  • Shih CC, Forman SJ, Chu P, Slovak M. Human embryonic stem cells are prone to generate primitive, undifferentiated tumors in engrafted human fetal tissues in severe combined immunodeficient mice. (razavihospital.ir)
  • In this study, we investigated the role of autophagy and apoptosis in Newcastle disease virus (NDV)-infected chicken cells and tissues. (immune-source.com)
  • In bottom line, we confirmed that autophagy brought about by genotype VII NDV infections was important for virus-like replication, NDV-induced apoptosis, and cell survival in buy 480-11-5 chicken cells and tissues. (immune-source.com)
  • Therefore, we propose that damage to and subsequent release of mtDNA elicits a protective signalling response that enhances nDNA repair in cells and tissues, suggesting that mtDNA is a genotoxic stress sentinel. (regenerativemedicine.net)
Lineages1
  • Endocrine Pancreas [1] "The transcription factor Pax6 functions in the specification and maintenance of the differentiated cell lineages in the endocrine pancreas. (edu.au)
Embryos5
  • ETiX embryos also develop beating hearts, trunk structures comprising a neural tube and somites, tail buds containing neuromesodermal progenitors and primordial germ cells, and gut tubes derived from definitive endoderm. (stemjnl.org)
  • Overall, ETiX embryos uniquely recapitulate natural embryos, developing further than any other stem-cell derived model, through multiple post-implantation stages and within extra-embryonic membranes. (stemjnl.org)
  • Moscona A. Cell suspensions from organ rudiments of chick embryos. (razavihospital.ir)
  • The T-box transcription factor eomesodermin (Eomes) has been implicated as an important component in germ layer induction and patterning in vertebrate embryos. (ox.ac.uk)
  • Conditional expression of Otx2 in these morphant embryos cell-autonomously rescues the formation of the ZLI at its correct location. (silverchair.com)
Sox1711
  • Laminin 411 markedly induced the expression of the genes Foxa2 and Sox17 , markers for pancreatic precursor cells, efficiently induced IPC differentiation from MSCs, and up-regulated insulin expression at both mRNA and protein levels. (biomedcentral.com)
  • We have used conditional gene deletion of mouse β-catenin in Sox17-positive embryonic and extra-embryonic endoderm as well as vascular endothelial progenitors to address the function of canonical Wnt signalling in cell lineage formation and patterning. (biologists.com)
  • Sox17 lineage tracing in the definitive endoderm (DE) shows a cell-autonomous requirement for β-catenin in midgut and hindgut formation. (biologists.com)
  • A set of markers (GSC, CK18, Gata4, Eomes, and Sox17) point to a mesendodermal nature of the epithelial cells with subsequent commitment to definitive endoderm (Sox17, Cdx2, nestin, and Islet1). (northwestern.edu)
  • 2020), Sox17 and β-catenin co-occupy Wnt-responsive en. (xenbase.org)
  • Sox17 and β-catenin co-occupy Wnt-responsive enhancers to govern the endoderm gene regulatory network. (xenbase.org)
  • Sox17 is a key transcriptional regulator of definitive endoderm development, and yet, its genomic targets remain largely uncharacterized. (xenbase.org)
  • Here, using genomic approaches and epistasis experiments, we define the Sox17 -governed endoderm GRN in Xenopus gastrulae. (xenbase.org)
  • We show that Sox17 functionally interacts with the canonical Wnt pathway to specify and pattern the endoderm while repressing alternative mesectoderm fates. (xenbase.org)
  • Sox17 -regulated endoderm transcriptome. (xenbase.org)
  • C) Sox17 -bound genes are enriched in the endoderm . (xenbase.org)
Pancreatic3
  • Promotes transdifferentiation of hepatocytes from mouse pancreatic cells (Shen et al. (stemcell.com)
  • One well-established example of transdifferentiation is the conversion of hepatocytes to pancreatic β-cells. (silverchair.com)
  • Here, we discuss how transcription factors, and their unique position as the gatekeepers of cellular identity, are exploited in cell reprogramming protocols by exploring work focusing on one reprogramming paradigm - the transdifferentiation of hepatocytes to pancreatic beta cells (β-cell). (silverchair.com)
Differentiate6
  • As a stem cell biologist, I aim to understand the mechanisms through which stem cells differentiate into progressively specialized cell types and to harness this knowledge to artificially generate pure populations of desired cell types from stem cells. (stanford.edu)
  • However, it has been notoriously challenging to guide PSCs to differentiate into a pure population of a given cell type. (stanford.edu)
  • To address this challenge, I mapped the cascade of branching lineage choices through which PSCs differentiate into various endodermal and mesodermal cell types. (stanford.edu)
  • Human embryonic stem cells (hESCs) derived from an early embryo can self-renew indefinitely and differentiate into a variety of cell types. (ashpublications.org)
  • We explore the recently described metabolic surge event that occurs as pluripotency is lost and stem cells commit to differentiate. (conditionmed.org)
  • Tissue development is a key process for life starting from the earliest embryonic stages during which cells differentiate into later organs composing an entire body. (exeter.ac.uk)
Germ1
  • The blastocyst comprises the inner cell mass (ICM), which gives rise to the three primary germ layers and consequently the fetus, and the trophectoderm (TE), which gives rise to the extraembryonic and placental tissue. (conditionmed.org)
Epithelium4
  • In the mouse, a transporting epithelium is established around the 8-16-cell stage through a process known as compaction where cell definition is lost and the outer cells of the embryo form tight junctions, giving rise to the blastocyst. (conditionmed.org)
  • Pivotal roles for eomesodermin during axis formation, epithelium-to-mesenchyme transition and endoderm specification in the mouse. (ox.ac.uk)
  • Ep-CAM (epithelial adhesion molecule, epithelial specific antigen, ESA) is a transmembrane glycoprotein expressed in the epithelium with a molecular weight of approximately 40 kDa, which functions as an epithelial cell adhesion molecule. (thermofisher.com)
  • Early specification of central epiblasts to a neural fate is influenced by fibroblast growth factor (FGF) production while induction of non-neural epithelium in the periphery occurs through wingless-type (Wnt) and bone morphogenic protein (BMP) expression. (biomedcentral.com)
Pluripotency2
  • To establish protocols for the safe and efficient differentiation of healthy cells for therapies, we must develop a better understanding of the dynamic continuum of metabolic states that span pluripotency and differentiation, and how to influence them. (conditionmed.org)
  • Molecular cues for pluripotency, genetic reprogramming of adult stem cells, and widely multipotent adult cells. (razavihospital.ir)
Specification4
  • The Notch/Lin-12/Glp-1 receptor family was found to be involved in the specification of cell fates during development in Drosophila and C. elegans. (wikipedia.org)
  • Cerberus and hhex are required in anterior endoderm for liver and cardiac specification. (xenbase.org)
  • Marker analysis as well as fate-mapping and chimera studies demonstrate for the first time that Eomes is required for specification of the definitive endoderm lineage. (ox.ac.uk)
  • Collectively, our experiments establish that Eomes is a key regulator of anteroposterior axis formation, EMT and definitive endoderm specification in the mouse. (ox.ac.uk)
Developmental6
  • We also highlight recent studies identifying the cis -regulatory elements such as enhancers at the global level and explain how their developmental activity is regulated by the cooperation of cell-type specific and ubiquitous transcription factors with extrinsic signals. (amegroups.org)
  • We further provide evidence to support the hypothesis that transcription factors (TFs) required to drive induction, differentiation, and maintenance of midbrain dopaminergic neural progenitor cells which give rise to terminally differentiated SNpc neurons are uniquely involved in both developmental patterning and metabolism, a dual function unlike other TFs that program neurons in other brain regions. (frontiersin.org)
  • This paper provides a cellular and developmental framework for understanding the selective vulnerability of SNpc dopaminergic cells to oxidative stress. (frontiersin.org)
  • Increasingly, complimentary cell culture techniques, which recapitulate the developmental microenvironment, are employed to coax cells to adopt new identities by indirectly regulating transcription factor activity via intracellular signalling pathways. (silverchair.com)
  • An essential component for these developmental processes but also for tissue regeneration and stem cell regulation is the communication of cells by chemical signalling. (exeter.ac.uk)
  • Wnt proteins regulate developmental processes, tissue regeneration and stem cell maintenance. (exeter.ac.uk)
Neural2
  • Recently, we showed that Wnt8a is transported on short, actin-based filopodia known as cytonemes to contact responding cells and activate signalling during neural plate formation in zebrafish (1).Wnt/ Ror2 signalling regulates the formation of these Wnt cytonemes (5). (exeter.ac.uk)
  • Neural plate induction is evident as thickening of epiblasts to form neuroectoderm. (biomedcentral.com)
PSCs1
Transcriptional4
  • The investigation of molecular mechanisms underlying transcriptional regulation, particularly in embryonic stem cells, has received increasing attention and involves the systematic identification of target genes and the analysis of promoter co-occupancy. (biomedcentral.com)
  • The RUNX2/BMI-1/SMAD2/3 complex is associated predominantly with genes not expressed in undifferentiated BG01V cells, consistent with the reported role of those TFs as transcriptional repressors. (biomedcentral.com)
  • In the developing embryo, TFs regulating the assembly/disassembly of transcriptional complexes and ultimately gene expression, are directed by complex extrinsic signalling processes which connect all cells within a multi-cellular organism to their environment. (amegroups.org)
  • Cellular identity is determined by the transcriptional profile which comprises the subset of mRNAs, and therefore proteins, being expressed by a cell at a given point in time. (silverchair.com)
Biology7
  • I then moved my laboratory to Stanford University as a Siebel Investigator and Instructor at the Stanford Institute for Stem Cell Biology & Regenerative Medicine. (stanford.edu)
  • These established tools enable new discoveries in fields such as immunology, inflammation and stem cell biology. (bdbiosciences.com)
  • While techniques for cell surface staining are relatively standard, optimal staining for intracellular markers often depends on the biology of the target protein. (bdbiosciences.com)
  • Welcome to the website of StemJournal , a vehicle for communicating outstanding stem cell research, including basic biology, clinical applications, disease modeling, computational and systems biology, and data science. (stemjnl.org)
  • Stem cell technologies have opened up new avenues in the study of human biology and disease. (ehu.eus)
  • They therefore represent a more realistic cellular environment for modeling the cell biology and pathology of the nervous system. (ehu.eus)
  • When this connection was made, it launched a whole new field," says Isidore Rigoutsos, Ph.D., professor of pathology, anatomy, and cell biology and director of the Computational Medicine Center at Thomas Jefferson University. (pharmaceuticalintelligence.com)
Development19
  • Although many differentiation protocols in the literature have been successful in mimicking lung development from stem cells, there has not been an examination of how a specific mutation impacts the differentiation process including its effects on the early endoderm, as well as the proximal and distal lung epithelial cell populations in the lung organoids. (nature.com)
  • It plays a major role in the regulation of embryonic development. (wikipedia.org)
  • The development of an efficient IPC induction system is a crucial step for the clinical application of IPCs for DM. (biomedcentral.com)
  • Stem Cells and Development , 21 (8), 1250-1263. (northwestern.edu)
  • In particular, due to the limited regenerative capacity of the adult nervous system, the therapeutic potential of stem cells has opened up new perspectives for development of treatment strategies for brain repair after injury or disease. (ehu.eus)
  • Here, we consider the metabolism of the early embryo through development, and look at the nutrient milieu within the developing stem cell niche. (conditionmed.org)
  • 2020). This places metabolism at the forefront of development and cell state decisions. (conditionmed.org)
  • The stimulus for the change of cell fate originates from extrinsic signals which set a cascade of intracellular processes in motion that eventually terminate at the genome leading to changes in gene expression and the development of alternate gene regulatory networks. (amegroups.org)
  • The development of the hematopoietic system has long served as model to understand how changes in gene regulatory networks drive the differentiation of the various blood cell types. (amegroups.org)
  • Embryonic development is tightly controlled at the level of gene expression. (amegroups.org)
  • Objectives Mesenchymal stem cells (MSCs) play an important role in the development and growth of tumor cells. (immune-source.com)
  • The use of these TFs during induction and differentiation may program ventral midbrain progenitor cells metabolically to higher ATP levels, allowing for the development of those specialized cell processes seen in terminally differentiated cells. (frontiersin.org)
  • The use of hPSCs provides great opportunities to model and study embryonic and human development. (biomedcentral.com)
  • However, creating a culture platform with spatiotemporal control of cell behavior to study signaling dynamics during development remains challenging. (biomedcentral.com)
  • The identification of key master regulator transcription factors (which distinguish one body part from another) during embryonic development has been central in developing transdifferentiation protocols. (silverchair.com)
  • Haeckel aggressively argued that the development of an embryo repeats or recapitulates the progressive stages of lower life forms and that by studying embryonic development one could thus study the evolutionary history of life on earth. (asu.edu)
  • The highly conserved family of Wnt proteins represents important regulators of cell behaviour, tissue development and homeostasis by inducing responses in a concentration-dependent manner. (exeter.ac.uk)
  • Due to the conserved nature of vertebrate cell behaviour, our results will be relevant to Wnt signalling during human embryonic development and could suggest novel vulnerabilities to Wnt-dependent diseases - a prerequisite for the development of novel therapeutics. (exeter.ac.uk)
  • The phenotype of Pax6-/- knockout mice suggests non-redundant functions of the transcription factor in the development of glucagon-expressing alpha-cells as this cell type is absent in the mutants. (edu.au)
Progenitor3
  • hPSC-derived CD34 + progenitor cells further developed to endothelial and smooth muscle cells with functionality. (ashpublications.org)
  • Our results suggest that combined modulation of signaling pathways may be an efficient means of differentiating hPSCs into functional CD34 + progenitor cells. (ashpublications.org)
  • Therefore, the therapeutic use of appropriate populations derived from stem and progenitor cells has been considered for cell-based therapies for neurodegenerative diseases, primarily Parkinson's disease, and brain injuries. (ehu.eus)
Microenvironment1
Visceral2
  • Tetraploid rescue experiments reveal that lack of β-catenin in the anterior visceral endoderm results in defects in head organizer formation. (biologists.com)
  • Surprisingly, wild-type posterior visceral endoderm (PVE) in midgut- and hindgut-deficient tetraploid chimera rescues the posterior body axis truncation, indicating that the PVE is important for tail organizer formation. (biologists.com)
Formation4
  • Teratoma formation by human embryonic stem cells is site dependent and enhanced by the presence of Matrigel. (razavihospital.ir)
  • Komura H, Ogita H, Ikeda W, Mizoguchi A, Miyoshi J, Takai Y. Establishment of cell polarity by afadin during the formation of embryoid bodies. (razavihospital.ir)
  • Formation of Ep-CAM-mediated adhesions have a negative regulatory effect on adhesions mediated by classic cadherins, which may have strong effects on the differentiation and growth of epithelial cells. (thermofisher.com)
  • The signalling molecules are delivered directly to the receiving cells by a direct-contact model and parameters such as cytoneme length or speed of filopodia formation dictate local Wnt concentration. (exeter.ac.uk)
Genes4
  • The search for an accurate and complete list of target genes for thousands of TFs and the elucidation of their complex interactions at promoter sites, particularly in embryonic stem (ES) cells, has gained increasing interest. (biomedcentral.com)
  • Genes Cells. (razavihospital.ir)
  • The production of terminally differentiated cells from such cells, therefore, requires successive changes of cell fates, meaning that genes relevant for the next stage of differentiation must be switched on and genes not relevant anymore must be switched off. (amegroups.org)
  • Ep-CAM functions as a homotypic calcium-independent cell adhesion molecule, and has a direct impact on cell cycle, proliferation and metabolism of epithelial cells and fibroblasts due to its ability to rapidly induce the proto-oncogene c-myc and the cell cycle regulating genes cyclin A and E. Ep-CAM mediates Ca2+-independent homotypic interactions. (thermofisher.com)
Endodermal4
  • After differentiating the mutant and corrected cells into lung organoids, we show expression of SFTPB mRNA during endodermal and organoid differentiation but the protein product only after organoid differentiation. (nature.com)
  • Here, we developed an hiPSC-based model to study the effect of collective cell migration in meso-endodermal lineage segregation and cell fate decisions through the control of space confinement using a detachable ring culture system. (biomedcentral.com)
  • Furthermore, the induction of collective cell migration at the colony edge using an endodermal induction media enhanced endodermal differentiation efficiency in association with cadherin switching, which is involved in the epithelial-mesenchymal transition. (biomedcentral.com)
  • Cadherin switching during collective cell migration enhances endodermal differentiation. (biomedcentral.com)
Molecular5
  • The molecular events leading to human embryonic stem cell (hESC) differentiation are the subject of considerable scrutiny. (northwestern.edu)
  • Therefore, after treatment, cells must be quickly fixed and subjected to stronger permeabilization conditions to allow the antibody to enter the nucleus and access the epitope within disrupted molecular complexes. (bdbiosciences.com)
  • Fundamental questions in organogenesis are related to the identification of a simplified model which is able to mimic the molecular mechanisms involved in pattern organization and cell fate determination as well. (razavihospital.ir)
  • Analyze stem cells and their derivatives using a wide range of cellular and molecular characterization methods, as validation is critical in iPSC research. (thermofisher.com)
  • However, the precise interplay between the molecular pathways responsible for nervous system induction are less apparent. (biomedcentral.com)
Mammalian1
  • The process of autophagy in mammalian cells involves six principal actions: initiation, nucleation, elongation, closure, maturation, buy 480-11-5 and degradation [27]. (immune-source.com)
Mouse7
  • Promotes osteogenic, adipogenic, and chondrogenic differentiation of mouse mesenchymal cells (Tropel et al. (stemcell.com)
  • Promotes differentiation of mature hepatocytes from mouse and human embryonic stem (ES) cells (Cai et al. (stemcell.com)
  • Inhibition of notch signaling inhibits the proliferation of T-cell acute lymphoblastic leukemia in both cultured cells and a mouse model. (wikipedia.org)
  • Recombinant Human Wnt-3a (Catalog # 5036-WN) induces alkaline phosphatase production by the MC3T3-E1 mouse preosteoblast cell line. (novusbio.com)
  • Measured by its ability to induce alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. (novusbio.com)
  • Staining of the TE-71 cell line with 0.06 µg of Rat IgG2a K Isotype Control APC (Product # 17-4321-81) (blue histogram) or 0.06 µg of Anti-Mouse CD326 (EpCAM) APC (purple histogram). (thermofisher.com)
  • In addition, mtDNA stress in TFAM-deficient mouse melanoma cells produces tumours that are more resistant to doxorubicin in vivo. (regenerativemedicine.net)
Foxa21
  • B) qPCR showing fold difference in expression between Gata4 treated animal caps and control animal caps grown to st. 33, induction of expression of myl7 , nr1h5 , hhex and marker of endoderm foxa2 is shown. (xenbase.org)
Regulator1
  • Cell cycle analysis showed an accumulation of tumor cells predominantly in 511296-88-1 manufacture G0/G1 phase with an increase in concentration of TD-MSCs, which was confirmed by increased mRNA expression of cell cycle negative regulator p21. (immune-source.com)
Precursors2
  • Embryonic precursors of liver and heart , whilst not sharing cellular origin, develop in close proximity through a dynamic series of inductive signaling events. (xenbase.org)
  • Ying QL, Stavridis M, Griffiths D, Li M, Smith A. Conversion of embryonic stem cells into neuroectodermal precursors in adherent Ferro F et al. (razavihospital.ir)
Epithelial cell2
  • Here we show the differentiation of patient specific iPSCs derived from a patient with SFTPB deficiency into lung organoids with mesenchymal and epithelial cell populations from both the proximal and distal portions of the human lung. (nature.com)
  • The immunogen used to generate the G8.8 antibody was the TE-71 thymic epithelial cell line. (thermofisher.com)
Mesenchymal cells1
  • Promotes osteogenic, adipogenic, and chondrogenic differentiation of human mesenchymal cells (Jaiswal et al. (stemcell.com)
Generate6
  • Current differentiation strategies typically generate heterogeneous cell populations unsuitable for basic research or clinical applications. (stanford.edu)
  • Stem cell research endeavors to generate specific subtypes of classically defined "cell types. (stanford.edu)
  • We thus efficiently generate artery and vein cells, introduce stem-cell-based toolkits for biosafety-level-4 virology, and explore the arterial tropism and cellular effects of Nipah and Hendra viruses. (stanford.edu)
  • A better understanding of the levers governing transcription factor activity benefits our ability to generate therapeutic cell types at will. (silverchair.com)
  • Here, we explore the evolution of reprogramming and directed differentiation approaches within the context of hepatocyte to β-cell transdifferentiation focussing on how the introduction of new techniques has improved our ability to generate β-cells. (silverchair.com)
  • LN metastases resist T cell-mediated cytotoxicity, induce antigen-specific regulatory T cells, and generate tumor-specific immune tolerance that subsequently facilitates distant tumor colonization. (stanford.edu)
Antibodies3
  • Fluorescent antibodies specific for cell surface markers can be combined with markers of apoptosis, proliferation and protein phosphorylation to determine which cell subsets respond to various stimuli or treatments. (bdbiosciences.com)
  • In addition, many fluorescent antibodies specific for key cell surface markers have been tested in several buffer systems to save time, sample and money. (bdbiosciences.com)
  • These simplified basic ChIP and novel Sequential ChIP protocols were successfully tested with a variety of antibodies with human embryonic stem cells, generated a number of novel observations for future studies and might be useful for high-throughput ChIP-based assays. (biomedcentral.com)
Adherent2
  • To establish the importance of specific cell signaling pathways to the production of pro-inflammatory cytokines from LPS-stimulated human monocytes, the NF-B inhibitor, Bay11, the p38 inhibitor, SB203580, the PI3K inhibitor, LY294002, and 607737-87-1 supplier the JNK inhibitor, SP 600125, were added to adherent human monocytes cultured in RPMI 1640 made up of 10% AB+ serum in flat bottom 96 well plates. (morainetownshipdems.org)
  • Such solutions are readily obtainable for two-dimensional adherent cell cultures, due to defined geometries with low flow rates, which enable precise characterization of fluid flow in microfluidic systems. (biomedcentral.com)
Induce3
  • Ligand proteins binding to the extracellular domain induce proteolytic cleavage and release of the intracellular domain, which enters the cell nucleus to modify gene expression. (wikipedia.org)
  • The compound can also be used in protocols for the chemical reprogramming of somatic cells to iPSCs.Shown to induce autophagy in cancer cell lines via a mechanism independent of AMPK inhibition. (tocris.com)
  • Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. (novusbio.com)
Somatic cells2
  • Our complete portfolio of stem cell research products and services provides optimized tools for each step of the stem cell workflow: from reprogramming somatic cells to iPSC expansion, validation, and differentiation. (thermofisher.com)
  • Reprogramming of somatic cells for iPSC generation can be accomplished with a number of technologies. (thermofisher.com)
Populations1
  • While western blotting and other methods are useful for the examination of single proteins expressed by entire cell populations, flow cytometry allows the detection of multiple proteins simultaneously at the level of individual cells. (bdbiosciences.com)
Posterior1
  • The posterior download drawing made easy dragons fantasy unleash your of self-containing die in reading beobachten stem and andere with Organ-derived Periodontal differentiation. (it-dresden.net)
Gene expression1
HPSCs1
  • A higher proportion of CD34 + cells (∼ 20%) could be derived from hPSCs by inhibition of mitogen-activated protein kinase (MAPK) extracellular signal-regulated protein kinase (MEK)/extracellular signal-regulated kinase (ERK) signaling and activation of bone morphogenic protein-4 (BMP4) signaling. (ashpublications.org)
Metabolism1
  • Alveolar type II (ATII) epithelial cells synthesize, secrete and recycle all components of surfactant and dysfunction in surfactant metabolism can result in a variety of pediatric lung diseases including respiratory distress syndrome and interstitial lung disease 3 . (nature.com)
Intracellular4
  • Intracellular flow cytometry is a powerful technique for the identification of cell types and the analysis of signaling and functional responses within cell lines and heterogeneous cell samples. (bdbiosciences.com)
  • For some cell types, such as Th17 and regulatory T cells (Tregs), definitive identification depends on the combined use of surface and intracellular markers such as cytokines or transcription factors. (bdbiosciences.com)
  • However, if they are trapped inside the cell, they can be stained as intracellular proteins using protein transport inhibitors such as BD GolgiStop™ (containing monensin) or BD GolgiPlug™ (containing brefeldin A). Cytokines are relatively accessible using the gentle fixation and permeabilization afforded by BD Cytofix/ Cytoperm™ Fixation and Permeabilization Solution. (bdbiosciences.com)
  • Wnts bind to the cell surface Frizzled family receptors in conjunction with low-density lipoprotein receptor-related protein family receptors (LRP5 or 6) resulting in the stabilization of intracellular beta -catenin levels (2). (novusbio.com)
Fibroblasts1
  • In cultured primary fibroblasts and cancer cells, the chemotherapeutic drug doxorubicin causes mtDNA damage and release, which leads to cGAS STING dependent ISG activation. (regenerativemedicine.net)