Embryonic stem cells cardiomyocytes. Medical search. Frequent questions

These dental stem cells are considered mesenchymal stem cells (MSCs) and possess different levels of capacities to become specific tissue forming cells. (bvsalud.org)

In this study, the interaction of mouse embryonic stem cells (mESC) with poly L-co-D, L lactic acid co-trimethylene carbonate (PLDLA-co-TMC) was assessed. (jscimedcentral.com)
physiologically based animals because a mouse embryonic stem cell line is used. (cdc.gov)
the EST, which assesses the effects of compounds on the differentiation of mouse embryonic stem cells into contracting cardiomyocytes, can be used to rank the potency of chemicals within a series of alkoxyacetic acid metabolites formed from The implementation of the European REACH (Registration, glycol ethers. (cdc.gov)

Induces hyperpolarization to the same magnitude as ACh in aortic value endothelial cells. (fishersci.com)
The surgical procedure for delivery of embryonic stem cell-derived endothelial cells to the ischemic hindlimb is demonstrated, with non-invasive tracking by bioluminescence imaging. (jove.com)
Does not induce differentiation of vascular smooth muscle cells or endothelial cells. (tocris.com)
In particular, the invention relates to methods of inducing differentiation of embryonic stem cells into muscle cells or vascular endothelial cells. (justia.com)
Therefore there remains a need for providing effective methods of inducing differentiation of stem cells into specific cell types, such as myocytes or endothelial cells. (justia.com)
BACKGROUND: In endothelial cells, phospholipase C (PLC) ß1-activated Ca2+ is a crucial second messenger for the signaling pathways governing angiogenesis. (bvsalud.org)
Angiogenic activities of GSLs in human umbilical vein endothelial cells (HUVECs) were evaluated. (bvsalud.org)
CONCLUSIONS: Overall, the fucose residue in GHCer dictated the glycan conformation for its complexing with TRAX to release TRAX-sequestered PLCß1, leading to Ca2+ mobilization in endothelial cells and enhancing angiogenesis in tumor microenvironments. (bvsalud.org)
Podoplanin (PDPN), a 36-kDa type I transmembrane O-glycoprotein, is expressed in normal cells, including renal epithelial cells (podocytes), lymphatic endothelial cells, and pulmonary type I alveolar cells, and in cancer cells, including brain tumors and squamous cell lung carcinomas. (bvsalud.org)

Embryonic stem cells (ESC) provide a great therapeutic potential for cardiac tissue. (jscimedcentral.com)
VE-CADHERIN is expressed transiently in early ISL1 + cardiovascular progenitor cells and facilitates cardiac differentiation. (nih.gov)
Cell therapy with embryonic stem cell-derived cardiomyocytes encapsulated in injectable nanomatrix gel enhances cell engraftment and promotes cardiac repair. (uab.edu)
Amend N, Thiermann H, Worek F, Wille T ( 2019 ) The arrhythmogenic potential of nerve agents and a cardiac safety profile of antidotes - A proof-of-concept study using human induced pluripotent stem cells derived cardiomyocytes (hiPSC-CM). Toxicol Lett 308:1-6. (multichannelsystems.com)
Kussauer S, David R, Lemcke H ( 2019 ) hiPSCs Derived Cardiac Cells for Drug and Toxicity Screening and Disease Modeling: What Micro- Electrode-Array Analyses Can Tell Us. (multichannelsystems.com)
Modeling genetic cardiac channelopathies using induced pluripotent stem cells - Status quo from an electrophysiological perspective. (uni-koeln.de)
You can record from neuronal or cardiac cultures, stem cells, or brain or cardiac slices. (multichannelsystems.com)
Progenitor cell therapy is being investigated for the treatment of damaged myocardium resulting from acute or chronic cardiac ischemia and for refractory angina. (southcarolinablues.com)
For individuals who have acute cardiac ischemia who receive progenitor cell therapy, the evidence includes 2 randomized controlled trials (RCTs) with 200 patients, numerous small RCTs, and meta-analyses of these RCTs. (southcarolinablues.com)
For individuals who have chronic cardiac ischemia who receive progenitor cell therapy, the evidence includes a nonrandomized comparative trial and systematic reviews of smaller RCTs. (southcarolinablues.com)
NKX2-5eGFP/w hESCs for isolation of human cardiac progenitors and cardiomyocytes. (edu.au)
In this review, we describe the cardiac differentiation from ES cells, iPS cells, and the current progress of using iPS cell-derived cardiomyocytes for heart disease modeling and for the development of therapeutic strategies. (biomedcentral.com)
The neomycin-resistant gene driven by the cardiac α-myosin heavy chain promoter was stably transfected into ES cells. (biomedcentral.com)
Human-induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) have shed great light on cardiac regenerative medicine and specifically myocardial repair in heart failure patients. (biomedcentral.com)
Cell replacement therapy also reversed ventricular remodeling, indicating the potential of iPSC-CMs for cardiac repair strategies. (biomedcentral.com)
The greatest goal in cardiac regenerative medicine is normally to make in large-scale, filtered cardiomyocytes which are ideal for cell transplantation highly. (bioinbrief.com)

Due to their plasticity and potentially unlimited capacity for self-renewal, embryonic stem cell therapies have been proposed for regenerative medicine and tissue replacement after injury or disease. (wikipedia.org)
In addition to their potential in regenerative medicine, embryonic stem cells provide a possible alternative source of tissue/organs which serves as a possible solution to the donor shortage dilemma. (wikipedia.org)
Transcription factor over-expression is a proven method for reprogramming cells to a desired cell type for regenerative medicine and therapeutic discovery. (nature.com)
During more recent years, his reseach has been conducted in the field of regenerative medicine, focussed on antigen expression and immune recognition of human embryonic stem cells and bioartificial heart valves, the latter tissues being of animal origin. (gu.se)
The increasing importance of iPS cells as regards their potential usefulness within regenerative medicine is widely known. (bioethicsobservatory.org)
Pluripotent stem cells have great potential for use in regenerative medicine and are an important tool for understanding developmental and disease mechanisms. (genengnews.com)

The influence of melatonin on the heart rhythm - An in vitro simulation with murine embryonic stem cell derived cardiomyocytes. (uni-koeln.de)
Role of natural-killer group 2 member D ligands and intercellular adhesion molecule 1 in natural killer cell-mediated lysis of murine embryonic stem cells and embryonic stem cell-derived cardiomyocytes. (tum.de)

Embryonic stem cells (ESCs), derived from the blastocyst stage of early mammalian embryos, are distinguished by their ability to differentiate into any embryonic cell type and by their ability to self-renew. (wikipedia.org)
Embryonic stem cells of the inner cell mass are pluripotent, meaning they are able to differentiate to generate primitive ectoderm, which ultimately differentiates during gastrulation into all derivatives of the three primary germ layers: ectoderm, endoderm, and mesoderm. (wikipedia.org)
When provided with the appropriate signals, ESCs initially form precursor cells that in subsequently differentiate into the desired cell types. (wikipedia.org)
According to a 2002 article in PNAS, "Human embryonic stem cells have the potential to differentiate into various cell types, and, thus, may be useful as a source of cells for transplantation or tissue engineering. (wikipedia.org)
Mouse ES cells have been widely utilized as an in vitro model to study cardiogenesis, as cardiomyocytes were found to spontaneously differentiate from ES cells after withdrawal of LIF (leukemia inhibitory factor), which functions to maintain the pluripotency of undifferentiated mouse ES cells [ 2 - 4 ]. (biomedcentral.com)
The defining characteristics of pluripotent stem cells, which include embryonic stem (ES) cells and induced pluripotent stem (iPS) cells, are straightforward: their ability to self-renew and differentiate into ectoderm, mesoderm, and endoderm germ layers. (genengnews.com)
The most widely accepted method for determining whether a population of cells is truly pluripotent is the teratoma assay, that is, verifying in vivo a cell population's ability to differentiate into each of the three germ layers via teratoma formation in mice. (genengnews.com)
Generally, when a stem cell culture is induced to differentiate, the differentiated population is analysed for particular cell types by expression of genes, markers or phenotypic analysis. (justia.com)
In particular, the induction of stem cells to differentiate into muscle cells (myocytes) is useful for muscle transplantation and therapeutic purposes, as well as providing potential human disease models in culture (e.g. for testing pharmaceuticals). (justia.com)
Background Embryonic stem cells (ESCs) can proliferate endlessly and are capable to differentiate into every cell lineages that make up the mature organism. (bioinbrief.com)
These ESCs have the ability to remain undifferentiated and proliferate indefinitely in vitro , while maintaining the potential to differentiate into all three embryonic germ layers [1,2]. (bioinbrief.com)
Presently, most protocols make use of stationary lifestyle to differentiate ESCs into cardiomyocytes [5-12]. (bioinbrief.com)
Our data shows that in contrast to static tradition, ESC ethnicities caused to differentiate toward cardiomyocytes in suspension bioreactors retain some capability to exhibit pluripotency indicators, to type. (bioinbrief.com)
A stem cell is commonly defined as a cell that has the ability to continuously divide and produce progeny cells that differentiate (develop) into various other types of cells or tissues. (bvsalud.org)

Embryonic stem cells (ESCs) are pluripotent stem cells derived from the inner cell mass of a blastocyst, an early-stage pre-implantation embryo. (wikipedia.org)
ESCs divide very frequently due to a shortened G1 phase in their cell cycle. (wikipedia.org)
Retinoblastoma proteins that inhibit the transcription factor E2F until the cell is ready to enter S phase are hyperphosphorylated and inactivated in ESCs, leading to continual expression of proliferation genes. (wikipedia.org)
Despite this difference in the cell cycle when compared to ESCs grown in media containing serum these cells have similar pluripotent characteristics. (wikipedia.org)
selectively promotes the differentiation of ESCs to cardiomyocytes in vitro between days 3-5. (tocris.com)
Extremely, nevertheless, our outcomes confirmed that the bioreactor differentiated ESCs maintained their capability to CL 316243 disodium salt manufacture exhibit pluripotency indicators, to type ESC-like colonies, and to generate teratomas upon transplantation, whereas the cells differentiated in adherent lifestyle dropped these features. (bioinbrief.com)
History Embryonic control cells (ESCs) are made from the internal cell mass (ICM) of pre-implantation embryos [1]. (bioinbrief.com)
This differentiation capacity makes ESCs an attractive cell source for cell/tissue alternative therapies for the treatment of human degenerative diseases. (bioinbrief.com)
The in vitro differentiation of ESCs into cardiomyocytes provides an opportunity to study the developmental aspects of cardiomyogenesis. (bioinbrief.com)

Cyclooxygenases Inhibitors Efficiently Induce Cardiomyogenesis in Human Pluripotent Stem Cells. (uni-koeln.de)

To take human organ generation via BC and transplantation to the next step, we reviewed current emerging organ generation technologies and the associated efficiency of chimera formation in human cells from the standpoint of developmental biology. (frontiersin.org)
The differentiated ES cell cultures are heterogeneous and contain undifferentiated ES cells, which could result in teratoma formation after transplantation into the host. (biomedcentral.com)
Clare's lab recently identified a population of progenitor/stem cells in mice that, on transplantation, is sufficient to generate a fully functional thymus. (eurostemcell.org)
Cell labeling, tracking, and molecular biology techniques indicated that the grafted cardiomyocytes survived in the rat heart 1 month after iPSC-CM transplantation. (biomedcentral.com)
Heart regenerative therapy, such as transplantation of myocytes [ 4 ] and cardiomyocytes to replace lost cells, has become a new strategy for the treatment of heart failure caused by MI [ 5 ]. (biomedcentral.com)
The induction of a specific differentiated cell type can be useful for transplantation or drug screening and drug discovery in vitro. (justia.com)
Such cell transplantation therapies would need the effective seeding of as many as 1 108 donor cardiomyocytes per individual [4]. (bioinbrief.com)

The study of promoter interactions in other hESC-derived cell types may be of utility in functional investigation of GWAS-associated regions. (manchester.ac.uk)
To account for this high level of functional variation, verifying the pluripotency of stem cells-from the generation of a new cell line through its expansion for an experiment-is crucial. (genengnews.com)
Animals injected with cell-free solution (control group) demonstrated significant left ventricular (LV) functional deterioration, whereas grafting of iPSC-CMs attenuated this remodeling process. (biomedcentral.com)
Myocardial infarction (MI), the most common heart disease, is characterized by a significant reduction in the number of functional cardiomyocytes and leads to the development of progressive heart failure. (biomedcentral.com)
Stem cells are undifferentiated cells which can give rise to a succession of mature functional cells. (justia.com)
of functional cardiomyocytes fully. (bioinbrief.com)

Promotes embryonic stem cell (ESC) differentiation into cardiomyocytes. (fishersci.com)
This kit, used in combination with the analysis of colony morphology, promotes the selection of high-quality, undifferentiated stem cell colonies, thereby reducing wasted time. (genengnews.com)

At this time I also began working with mouse and human embryonic stem cells resulting in the publication of some of the first papers describing the osteogenic differentiation of mouse ES and human cells in vitro and in vivo . (nottingham.ac.uk)

Progenitor cell therapy describes the use of multipotent cells of various cell lineages (autologous or allogeneic) for tissue repair and/or regeneration. (southcarolinablues.com)
Specifically, human iPS cells could be utilized to generate patient-specific lineages for a variety of translational research. (biomedcentral.com)
What makes characterization challenging is that heterogeneity within a given stem cell population can cause variability in the differentiation into important downstream lineages, such as cardiomyocytes and hepatocytes. (genengnews.com)
The process of differentiation in stem cells involves selective development of immature cells to committed and fully mature cells of various lineages. (justia.com)

Embryonic precursors of liver and heart , whilst not sharing cellular origin, develop in close proximity through a dynamic series of inductive signaling events. (xenbase.org)
During differentiation the expression of stem cell specific genes and markers are often lost and cells acquire gene expression profiles of somatic cells or their precursors. (justia.com)

Among the current biomaterial applications, the use of biopolymers as cell scaffolds could aid the development of stem cell-based therapies aimed at replacing tissue loss due to injuries or diseases, such as during myocardial infarction. (jscimedcentral.com)
The cells are being studied to be used as clinical therapies, models of genetic disorders, and cellular/DNA repair. (wikipedia.org)
hES cells also serve as a basis for cerebral organoid models (Figure C) to better understand e.g. the mechanisms of cognitive impairment and radiation necrosis as severe side effects of radiation therapies. (gsi.de)
This can be applied in cGMP manufacture and enhances directed differentiation or cell conversion to support the development of scalable off-the-shelf therapies for diseases with a high unmet clinical need. (technologynetworks.com)
This represents a unique opportunity to transform the development of existing cell therapies and will become instrumental in our bid to engineer scalable off-the-shelf therapies for diseases with a high unmet clinical need. (technologynetworks.com)
Literature review: This review summarizes current knowledge, barriers, and challenges in the clinical use of adult stem cells, scaffolds, and growth factors for the development and evaluation of regenerative endodontic therapies. (bvsalud.org)
This explains why researchers are now focusing attention on developing stem cell therapies using postnatal stem cells donated by the patients themselves or their close relatives. (bvsalud.org)

In tissue engineering, the use of stem cells are known to be of importance. (wikipedia.org)
For this purpose, we use human stem cells that play a pivotal role in tissue development, maintenance and function. (gsi.de)
I returned full time to Imperial College in 1999 to take up a lectureship in cell biology and tissue engineering and was also actively involved in establishing the Tissue Engineering Centre. (nottingham.ac.uk)
I was also affiliated with the Wolfson Centre for Stem Cells, Tissue Engineering and Modelling (STEM), part of the Centre for Biomolecular Sciences, which is now the Biodiscovery Institute. (nottingham.ac.uk)
My research interests focus mainly on stem cells (embryonic, 'adult' and fetal origins) and their applications in tissue engineering, particularly the osteoblast and bone tissue. (nottingham.ac.uk)
Dr. Jun's research group focuses on nanostructured biomaterials and stem-cell-based tissue regeneration to develop an innovative strategy to treat various diseases. (uab.edu)
The platform utilizes data from more than 100 human cell/tissue types (available via the ENCODE and Epigenome Roadmap consortia) to accurately define culture conditions that can maintain the cell identity or induce cell conversion. (technologynetworks.com)
Embryonic stem (ES) cells are derived from the embryo and are pluripotent, thus possessing the capability of developing into any organ, cell type or tissue type. (justia.com)
Methods of inducing differentiation in stem cells and muscle cells produced therefrom may be used for the study of cellular and molecular biology of tissue development, for the discovery of genes and proteins such as differentiation factors that play a role in tissue development and regeneration. (justia.com)
The key elements of tissue engineering are stem cells, morphogen, and a scaffold of extracellular matrix. (bvsalud.org)
Postnatal stem cells have been sourced from umbilical cord blood, umbilical cord, bone marrow, peripheral blood, body fat, and almost all body tissues, including the pulp tissue of teeth 8 . (bvsalud.org)

EpiMOGRIFY-predicted factors are able to maintain astrocytes and cardiomyocytes in vitro in chemically defined media and promote the generation of astrocytes and cardiomyocytes from neural progenitors and embryonic stem cells, respectively. (technologynetworks.com)

These germ layers generate each of the more than 220 cell types in the adult human body. (wikipedia.org)
ES cells are pluripotent cells, which mean that they can give rise to all differentiated cell types derived from all three germ layers. (bvsalud.org)

Efficient Endoderm Induction from Human Pluripotent Stem Cells by Logically Directing Signals Controlling Lineage Bifurcations. (edu.au)
The kit contains a set of azide-free, fluorochrome-conjugated GloLIVE antibodies that recognize two positive markers and one negative marker of human pluripotent stem cells. (genengnews.com)

Pluripotency distinguishes embryonic stem cells from adult stem cells, which are multipotent and can only produce a limited number of cell types. (wikipedia.org)
The most valuable cells for regenerative endodontics are postnatal or adult stem cells. (bvsalud.org)
Based on their origin, there are two main types of stem cells - embryonic stem cells (ES cells) and postnatal or adult stem cells (AS cells). (bvsalud.org)

To address these difficulties, R&D Systems has developed new tools that aid in the verification of stem cell pluripotency during derivation, maintenance, and expansion. (genengnews.com)

Human embryos reach the blastocyst stage 4-5 days post fertilization, at which time they consist of 50-150 cells. (wikipedia.org)
Isolating the inner cell mass (embryoblast) using immunosurgery results in destruction of the blastocyst, a process which raises ethical issues, including whether or not embryos at the pre-implantation stage have the same moral considerations as embryos in the post-implantation stage of development. (wikipedia.org)
Embryonic stem (ES) cells are naturally derived from early stage embryos and induced pluripotent stem (iPS) cells are reprogrammed from somatic cells with overexpression of four reprogramming factors, Oct4, Sox2, Klf4 and c-Myc. (biomedcentral.com)
These cells have been specially derived from human embryos (embryonic stem cells) or from reprogrammed adult cells (iPS cells). (bioethicsobservatory.org)

One way we did this was by converting adult fibroblasts, or skin cells, into interneurons. (lu.se)

Fourier transform infrared microspectroscopy identifies early lineage commitment in differentiating human embryonic stem cells. (edu.au)
Whilst differentiation of some lineage specific stem cells can be induced with a degree of certainty, a differentiation outcome of a population of pluripotent stem cells is less predictable. (justia.com)

mEScell growth and differentiation on PLDLA-co-TMC membrane, demonstrated the cyto compatibility of this polymer, and provided evidence of minimal to no toxicity on the cells, allowing adhesion and cell proliferation, as well as differentiation into cardiomyocytes. (jscimedcentral.com)
Notch1, Notch2, and Epstein-Barr virus-encoded nuclear antigen 2 signaling differentially affects proliferation and survival of Epstein-Barr virus-infected B cells. (tum.de)
These antibodies are added to live, unfixed cells in a single-step assay that takes only 30 minutes and has no adverse effects on stemness or proliferation. (genengnews.com)
Although a small percentage of the cells may be capable of proliferation [3], this CL 316243 disodium salt manufacture is usually not sufficient for regeneration after myocardial injury. (bioinbrief.com)

Self-renewal conditions must prevent the cells from clumping and maintain an environment that supports an unspecialized state. (wikipedia.org)
Both ES cells and iPS cells are pluripotent stem cells with capabilities of indefinite self-renewal and can be differentiated into almost all cell types of the body, which make them valuable for studying early developmental biology, for modeling and as therapy for human diseases. (biomedcentral.com)

Tahrir FG, Gordon J, Feldman AM, Cheung J, Khalili K, Mohseni Ahooyi T ( 2019 ) Evidence for the impact of BAG3 on electrophysiological activity of primary culture of neonatal cardiomyocytes. (multichannelsystems.com)

It has now been shown that if iPS cells obtained from the skin or bone marrow of a person are injected back into the same person, they can generate tumours and trigger inflammation. (bioethicsobservatory.org)
Conventional bone marrow-derived dendritic cells contribute to toll-like receptor-independent production of alpha/beta interferon in response to inactivated parapoxvirus ovis. (tum.de)

The main research theme of her lab is neural stem cells, and the molecular pathophysiology of Huntington's Disease . (eurostemcell.org)

Somatic cell therapy is viewed as a more conservative, safer approach because it affects only the targeted cells in the patient, and is not passed on to future generations. (omicsonline.org)
Such single gene disorders are good candidates for somatic cell therapy. (omicsonline.org)

A leading contributing factor to metastasis and treatment resistance is the heterogeneity and plasticity of the cells within tumors 6 . (nature.com)

Noninvasive detection and imaging of molecular markers in live cardiomyocytes derived from human embryonic stem cells Biophysical Journal. (nottingham.ac.uk)
However, the molecular pathways that lead to specification and terminal differentiation of specific cell types, such as myocytes, from embryonic stem cells during development are not entirely clear. (justia.com)

However, though BC is emerging as a potential organ transplant option, challenges regarding organ size scalability, immune system incompatibilities, long-term maintenance, potential evolutionary distance, or unveiled mechanisms between donor and host cells remain. (frontiersin.org)
The possible donor-host rejection of human ES cells is another concern 3 . (bvsalud.org)

1 Treatment with progenitor cells (i.e., stem cells) offers potential benefits beyond those of standard medical care, including the potential for repair and/or regeneration of damaged myocardium. (southcarolinablues.com)
There are limited numbers of publications about ES cells in pulp regeneration, due to the restricted policies regarding ES cell research over the past few years. (bvsalud.org)

A) Colonies of human embryonic stem cells, bright field microscopy, scale bar: 100µm. (gsi.de)
Manually picking colonies that contain pluripotent stem cells is possible through an analysis of colony morphology. (genengnews.com)
Pluripotency of live human stem cell colonies was verified using a NorthernLights™ (NL) 493-conjugated Mouse Anti-Human SSEA-4 Monoclonal Antibody (green) and a NL557-conjugated Mouse Anti-Human TRA-1-60(R) Monoclonal Antibody (red). (genengnews.com)

These conditions include growing the cells to high or low density, changing media, introducing or removing cytokines, hormones and growth factors, creating an environment which suits differentiation toward a specific cell type, such as providing a suitable substrate. (justia.com)

Researchers are currently focusing heavily on the therapeutic potential of embryonic stem cells, with clinical use being the goal for many laboratories. (wikipedia.org)

A well-established method of cardiomyocyte differentiation from PSCs provides an ideal source of cardiomyocytes and makes regenerative therapy for myocardial muscle damage a promising strategy. (biomedcentral.com)

Gene and Cell Therapy for Sinus and AV Nodal Dysfunction. (heartandcoeur.com)
For individuals who have refractory angina who receive progenitor cell therapy, the evidence includes phase 2 trials and a phase 3 pivotal trial. (southcarolinablues.com)
Additional larger trials are needed to determine whether progenitor cell therapy improves health outcomes in patients with refractory angina. (southcarolinablues.com)
In addition, we summarize the recent direct reprogramming of cardiomyocytes from fibroblast cells, which provides another method for potential heart disease therapy. (biomedcentral.com)
Objectives ethical difficulties therapy solved by iPS cells? (bioethicsobservatory.org)

check the tag ADOLESCENCE HN - 2008 BX - Nutrition in Adolescence FX - Adolescent Nutrition Physiology MH - Peritoneal Stomata UI - D054048 MN - A01.047.025.600.700 MN - A10.810 MS - Natural openings in the subdiaphragmatic lymphatic plexus in the PERITONEUM, delimited by adjacent mesothelial cells. (bvsalud.org)

Placing the cells under conditions which induce specific cell types has been one form of an attempt to regulate the differentiation outcome. (justia.com)
culturing a stem cell in the presence of an embryonic cell and/or extracellular medium of an embryonic cell, under conditions that induce differentiation of the stem cell. (justia.com)
This study demonstrates that suspension bioreactor tradition systems do indeed possess the ability to prevent differentiation, and actually induce 'transient' pluripotency within a defined differentiation protocol, presumably due to influence of shear stress on the cells. (bioinbrief.com)
Rare mutations of ADAM17 from TOFs induce hypertrophy in human embryonic stem cell-derived cardiomyocytes via HB-EGF signaling. (cdc.gov)

Kraushaar U, Guenther E ( 2019 ) Assay Procedures for Compound Testing of hiPSC-Derived Cardiomyocytes Using Multiwell Microelectrode Arrays. (multichannelsystems.com)

Pluripotency factors Oct4 and Nanog play a role in transcriptionally regulating the embryonic stem cell cycle. (wikipedia.org)
Moreover, colony morphology is not truly indicative of pluripotency, since cells that are not fully reprogrammed can have similar morphology. (genengnews.com)
The GloLIVE™ Human Pluripotent Stem Cell Live Cell Imaging Kit and individual GloLIVE antibodies available from R&D Systems allow you to verify the pluripotency status of your stem cells by monitoring the expression of both positive and negative markers ( Figure 1 ). (genengnews.com)
Despite its broad use as the most common assay for assessing the pluripotency of stem cells, the teratoma assay has limitations. (genengnews.com)
The advancement of effective ESC difference protocols within suspension system bioreactors needs a even more comprehensive understanding of the affects of shear factors on the control of pluripotency and difference in pluripotent control cells. (bioinbrief.com)
Nevertheless, since we have previously observed that the suspension bioreactor environment enhances ESC pluripotency, while suppressing differentiation effectiveness [17], we wanted to investigate whether this trend would also happen in during cardiomyocyte differentiation. (bioinbrief.com)

Examination of gene expression in st. 43 AC/AE explants by RT-PCR reveals expression of liver (fabp1), intestine (fabp2), pancreas (pdia2 and pdx1 , but not ins and amy2a), thyroid/lung (nkx2-1) as well as ventricular cardiomyocyte (myl3) markers. (xenbase.org)
Mouse ES cell-derived EBs were dissociated using collagenase, followed with a modified procedure by Isenberg and Klockner in 1982 to prepare the calcium-tolerant ventricular myocytes [ 18 ]. (biomedcentral.com)

On the other hand, a chimera is defined as an organism in which cells from two or more different organisms have contributed. (frontiersin.org)
Particularly, human embryonic stem cells (hES cells), which can give rise to all tissues of an organism (Figure A), are used to generate mature cells of the heart (Figure B) allowing us to study radiation/microgravity-induced cardiovascular alterations, which may occur during long-term space missions. (gsi.de)

The induction of cardiomyocyte differentiation in stem cells is especially useful in developing therapeutic methods and products for heart disease and abnormal heart conditions. (justia.com)

The primitive streak gene Mixl1 is required for efficient haematopoiesis and BMP4-induced ventral mesoderm patterning in differentiating ES cells. (edu.au)

I cover mainly cell biology and physiology. (nottingham.ac.uk)
Professor Elena Cattaneo is Director of the Laboratory of Stem Cell Biology and Pharmacology of Neurodegenerative Diseases at the Department of Pharmacological Sciences, as well as a co-founder and first appointed Director of UniStem , the Centre for Stem Cell Research of the University of Milano. (eurostemcell.org)
Combining cell biology and electrophysiology, his work has the potential to create personalized disease models for future research. (lu.se)

The characterization of this material was made by means of SEM, DSC, TGA, GPC, Tensile Test, Viability Assay, and stem cells behavior by qPCR and Immunofluorescence techniques. (jscimedcentral.com)
The teratoma assay is also inherently variable, depending on the site of cell inoculation and the mouse strain used. (genengnews.com)
METHODS: Globo-series glycosphingolipids (GSLs), including GHCer and stage-specific embryonic antigen-3 ceramide (SSEA3Cer), were analyzed using enzyme-linked immunosorbent assay (ELISA) and Biacore for their binding with TRAX. (bvsalud.org)

Cyclin-dependent kinases such as CDK2 that promote cell cycle progression are overactive, in part due to downregulation of their inhibitors. (wikipedia.org)

The Effect of Antiarrhythmic Drugs on the Beat Rate Variability of Human Embryonic and Human Induced Pluripotent Stem Cell Derived Cardiomyocytes. (uni-koeln.de)

Rapid cell division allows the cells to quickly grow in number, but not size, which is important for early embryo development. (wikipedia.org)
Endocardium differentiation through Sox17 expression in endocardium precursor cells regulates heart development in mice. (nih.gov)
Tumor initiation, development, and relapse may be closely associated with cancer stem cells (CSCs). (nature.com)
The thymus plays a central role in the development of the body's immune system as it is the main site in which T cells are generated. (eurostemcell.org)
Owen Rackham, PhD, Assistant Professor at Duke-NUS Medical School, Singapore, and Co-founder and CTO, Mogrify, said: "Building on our development of the Mogrify technology to identify transcriptomic switches for cell conversion, this new technology published in Cell Systems adds an important layer of epigenetic specificity in our continuous search to drive cell identity with an increasing level of control. (technologynetworks.com)
In vitro toxicity data of these metabolites derived in the development of validated and accepted in vitro and in silico embryonic stem cell test were used as input in the PBK model to extrapolate in vitro concentration-response curves to predicted approaches is urgently needed. (cdc.gov)
The major areas of research that might have application in the development of regenerative endodontic techniques are (a) postnatal stem cells, (b) scaffold materials, (c) morphogen/growth factors, (d) implantation. (bvsalud.org)

All research areas are substantiated by analyses (Figure D) monitoring the acute radiation-induced cytogenetic damage and the long-term genetic stability of cells. (gsi.de)
My research continues to focus on stem cells and osteoblasts and in particular 3D cell culture models and microenvironments. (nottingham.ac.uk)
Chris' primary research focus is on using experimental and clinical data to predict how heart cells, the engine of every heartbeat, respond to genetic mutations and to drugs. (swansea.ac.uk)
Our findings may have implications for research on liver differentiation from embryonic stem cells. (xenbase.org)
The research underpinning this novel computational approach has recently been published in Cell Systems* (Kamaraj et al. (technologynetworks.com)
A conclusion This scholarly research demonstrates that although cardiomyocyte difference can CL 316243 disodium salt manufacture end up being attained in stirred suspension system bioreactors, the addition of moderate boosters is certainly not really sufficient to power comprehensive difference as liquid shear factors show up to maintain a subpopulation of cells in a transient pluripotent condition. (bioinbrief.com)
An important aspect of ESC research focuses on elucidating the mechanisms of differentiation from the pluripotent ESC to numerous terminally differentiated cell types. (bioinbrief.com)
Andreas' research focuses on generating brain cells, specifically interneurons, in the lab, which has significant implications for the study of neurodegenerative and neuropsychiatric disorders. (lu.se)

Non-thermal disruption of β-adrenergic receptor-activated Ca2+ signalling and apoptosis in human ES-derived cardiomyocytes by microwave electric fields at 2.4 GHz. (swansea.ac.uk)
The FRET analysis also indicated the disruption of colocalization of TRAX and PLCß1 in cells by GHCer derived from EVs. (bvsalud.org)

At the Stem Cell Differentiation and Cytogenetics Group, we are interested in the effects of ionizing radiation on organs such as the brain, the heart, and the lung trying to elucidate the risks for patients treated with ionizing radiation as well as for astronauts, who are inevitably exposed to ionizing radiation on their missions into space. (gsi.de)
Finally, using primary lung stem cells or lymphocytes from different donors, we study the effects of radon on patients, who are treated in radon spas or baths to alleviate rheumatic diseases. (gsi.de)
In the last 3 years, several protocols have been developed to derive various types of lung cells from iPS and embryonic cells. (bioethicsobservatory.org)

RNA-seq following PCR-based sorting reveals rare cell transcriptional signatures. (nature.com)

Anatomy48

Organisms12

Diseases5

Chemicals and Drugs74

Analytical, Diagnostic and Therapeutic Techniques and Equipment54

Psychiatry and Psychology1

Phenomena and Processes64

Disciplines and Occupations4

Technology, Industry, Agriculture1

Information Science2

Mesenchymal stem1

Mouse embryonic3

Endothelial cells9

Cardiac16

Regenerative medicine6

Murine embryonic2

Differentiate14

ESCs9

Cardiomyogenesis1

Transplantation7

Functional6

Promotes2

Differentiation of mouse1

Lineages4

Precursors2

Therapies7

Tissue11

Progenitors1

Germ2

Human Pluripotent Stem2

Adult stem3

Derivation1

Embryos4

Fibroblasts1

Lineage2

Proliferation4

Self-renewal2

Neonatal1

Bone marrow2

Neural1

Somatic2

Heterogeneity1

Molecular2

Donor2

Regeneration2

Colonies3

Substrate1

Therapeutic potential1

Myocardial1

Therapy5

Mesothelial cells1

Induce4

20191

Pluripotency6

Ventricular2

Organism2

Induction1

Gene1

Biology3

Assay3

Inhibitors1

Variability1

Development7

Research8

Disruption2

Lung3

Transcriptional1