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  • 2017
  • The table to the right includes counts of all research outputs for State Key Laboratory of Cell Biology (SKLCB), IBCB SIBS CAS published between 1 November 2017 - 31 October 2018 which are tracked by the Nature Index. (natureindex.com)
  • genes
  • In the present study, we show that in H358 non-small cell lung cancer cells, ZEB1 downregulates EpCAM (coding for an epithelial cell adhesion molecule), ESRP1 (epithelial splicing regulatory protein), ST14 (a membrane associated serine protease involved in HGF processing) and RAB25 (a small G-protein) by direct binding to these genes. (mdpi.com)
  • While many immunologically relevant genes undergo alternative splicing, the role of regulated splicing in T cell immune responses is largely unexplored, and the signaling pathways and splicing factors that regulate alternative splicing in T cells are poorly defined. (frontiersin.org)
  • We show that SC35 colocalizes with RNA polymerase II in activated T cells and spatially overlaps with H3K27ac and H3K4me3, which mark transcriptionally active genes. (frontiersin.org)
  • Several T cell genes, such as CD44 and CD45 , undergo alternative splicing to produce distinct protein isoforms ( 7 , 8 ). (frontiersin.org)
  • The coordinated development of the nervous system requires fidelity in the expression of specific genes determining the different neural cell phenotypes. (frontiersin.org)
  • At the same time, stem cell decisions regarding fate commitment and differentiation are highly dependent on the temporospatial expression of specific genes contingent on the developmental stage of the nervous system. (frontiersin.org)
  • These signals induce expression of genes involved in lineage commitment, differentiation, maturation, migration and cell survival. (frontiersin.org)
  • A bivalent chromatin structure marks key developmental genes in embryonic stem cells. (scienceopen.com)
  • They have been differentiated to bone-forming osteoblasts, cartilage-forming chondrocytes, fat-forming adipocytes and neural cells and to attachment-independent spherical clusters expressing genes associated with pancreatic islets. (biologists.org)
  • chromatin
  • In this study, we used a strategy combining the genetic manipulation of mouse embryonic stem cells with affinity purification and quantitative mass spectroscopy utilizing stable isotope labeling with amino acids in cell culture to study the interactome of chromatin-bound β-catenin with and without Wnt3a stimulation. (mcponline.org)
  • Thus, β-catenin is stabilized, translocates to the nucleus, and associates with TCF factors and with various proteins that are implicated in chromatin structure and RNA polymerase II regulation ( 1 , 2 ). (mcponline.org)
  • In mES 1 cells, the epigenetic regulation of gene expression has been shown to take place at the levels of DNA methylation, histone modification, nucleosome packaging and rearrangement, and higher order chromatin organization ( 4 ). (mcponline.org)
  • We find that both structural heterochromatin and gene silencing can be stably maintained over an ∼10-kb domain for up to hundreds of cell divisions in the absence of genomic sequences required for heterochromatin establishment, demonstrating the long-term persistence and stability of this chromatin state. (genetics.org)
  • THE establishment and maintenance of alternative chromatin states over the course of multiple cell divisions requires the complex integration of both genomic and nongenomic signals (reviewed in Straub and Becker 2008 ). (genetics.org)
  • In addition to establishing a functional link between chromatin structure and gene expression state, these studies demonstrated that genetically identical cells can achieve alternate gene expression states that are stably maintained through cell division, thereby supporting an epigenetic mechanism of inheritance. (genetics.org)
  • In these examples, the inheritance of alternative chromatin states cannot be uncoupled from the DNA sequences that direct establishment (or reassembly) of chromatin structure following each successive cell division. (genetics.org)
  • By chromatin immunoprecipitation assay, we demonstrated that H3K27me3 was significantly enriched at the DLC1 promoter region of a DLC1-nonexpressing HCC cell line, MHCC97L. (plos.org)
  • We show for the first time that nuclear PKC-θ co-exists with SC35 in the context of the chromatin template and is a key regulator of SC35 in T cells, directly phosphorylating SC35 peptide residues at RNA recognition motif and RS domains. (frontiersin.org)
  • By use of high resolution tiling microarrays in conjunction with immunoprecipitation of methylated DNA (MeDIP) or modified histones (chromatin IP, ChIP), our study revealed highly distinct landscapes of epigenetic modifications associated with latent KSHV infection in several tumor-derived cell lines as well as de novo infected endothelial cells. (scienceopen.com)
  • Endothelial Cells
  • VEGF receptors (VEGFR1 and VEGFR2) are present on vascular endothelial cells and their signaling is mediated by receptor dimerization leading to autophosphorylation of the cytosolic domains of the receptors. (biologists.org)
  • Gene Expression
  • Endoderm-Specific Gene Expression in Embryonic Stem Cells Differentiated to Embryoid Bodies," Exp Cell Res. (patentgenius.com)
  • The changes in phenotype and function that characterize the differentiation of naïve T cells to effector and memory states are underscored by large-scale, coordinated, and stable changes in gene expression. (frontiersin.org)
  • Alternative splicing is a key mechanism for generating protein diversity and regulating gene expression and, therefore, plays an important role in cell function and development. (frontiersin.org)
  • tumor
  • Our proof-of-principle experiments show that β-catenin can recruit the H3K4me2/1 demethylase LSD1 to regulate the expression of the tumor suppressor Lefty1 in mouse embryonic stem cells. (mcponline.org)
  • The epithelial-to-mesenchymal transition (EMT), which converts epithelial cells into an elongated, motile and invasive phenotype, is thought to be a critical step in the dissemination of tumor cells during metastasis [ 1 , 2 ]. (mdpi.com)
  • Neuroblastoma is an embryonal extracranial solid tumor originating from undifferentiated neural crest cell and it is the most common among children. (intechopen.com)
  • New insights into tumor biology are leading to the development of novel therapeutic approaches, which include small-molecule inhibitors as well as epigenetic approaches, noncoding-RNA, and cell-based immunologic therapies. (intechopen.com)
  • regulation
  • Ameloblastin-positive dental epithelial cells were detected following the temporal regulation of BMP4. (biomedcentral.com)
  • Changes in mitochondrial regulation might also be driven by genetic developmental programmes, reflecting the typical energy requirements of these cell types in vivo as opposed to the energy requirements in culture. (biologists.org)
  • Epigenetic regulation is organized at multiple levels, involving primarily DNA methylation and selective histone modifications in cancer cells. (mdpi.com)
  • While most research has focused on transcriptional regulation of immune responses, alternative splicing of pre-mRNA is an emerging theme in the regulation of T cell function ( 5 , 6 ). (frontiersin.org)
  • An interplay between the above, as well as basic cell processes, such as transcription regulation, DNA replication, cell cycle regulation and DNA repair therefore determine the accuracy and function of neuronal connections. (frontiersin.org)
  • cancer cells
  • We reported that cancer cells-secreted HMGB1 employs MSC into tumors. (nii.ac.jp)
  • The mRNA levels of LSD1 and β -catenin are inversely correlated with the levels of Lefty1 in pancreas and breast tumors, implying that this mechanism is common to mouse embryonic stem cells and cancer cells. (mcponline.org)
  • It is activated in cancer cells and is involved in invasion, metastasis and stem-like properties. (mdpi.com)
  • In addition, several studies evaluated the 5-methylcytosine (5 mC) distribution patterns, which distinguish cancer cells from normal cells, and how CpG methylation contributes to the oncogenic phenotype. (intechopen.com)
  • epithelial cells
  • The epithelial to mesenchymal transition (EMT) enables epithelial cells with a migratory mesenchymal phenotype. (mdpi.com)
  • E-cadherin is a well-characterised single-pass transmembrane Type I cadherin that is primarily expressed on epithelial cells and contains a cytoplasmic domain of 150aa and an extracellular domain of 550aa containing five EC repeats, each of approximately 110aa [ 1 , 2 ]. (hindawi.com)
  • therapeutic
  • Debates the issue of whether the United States Congress should use tax money to fund therapeutic cloning and embryonic stem-cell research. (ebscohost.com)
  • Importantly, we found that DZNep treatment inhibited HCC cell migration through disrupting actin cytoskeleton network, suggesting the therapeutic potential of DZNep in targeting cancer metastasis. (plos.org)
  • For these reasons, unraveling the MYC-mediated mechanism in those cells is fundamental to exploit their full potential and to identify therapeutic targets. (frontiersin.org)
  • pathways
  • The researchers say they should now be in a position to better understand how the three stem cell types interact to enable the embryo to develop, by experimentally altering biological pathways in one cell type and seeing how this affects the behaviour of one, or both, of the other cell types. (eurekalert.org)
  • ATP generation in aerobic cells is divided between two main pathways: anaerobic glycolysis and mitochondrial oxidative phosphorylation. (biologists.org)
  • mRNA
  • Conversely, transient overexpression of GFP-EZH2 in DLC1-expressing Huh7 cells reduced DLC1 mRNA level with a concomitant enrichment of EZH2 on DLC1 promoter. (plos.org)
  • phenotype
  • For instance, CD4 + T cells that adopt the Th2 phenotype secrete IL-4, which elicits maturation of the B cell-mediated antibody response while regulatory T cells (Tregs) function to maintain immune homeostasis through the secretion of immunosuppressive cytokines, including IL-10 and TGF-β. (frontiersin.org)
  • E-cadherin is the primary cell adhesion molecule within the epithelium, and loss of this protein is associated with a more aggressive tumour phenotype and poorer patient prognosis in many cancers. (hindawi.com)
  • We propose that aberrant E-cadherin expression is a critical contributing factor to neoplasia and the early stages of tumorigenesis in the absence of EMT by altering growth factor response of the cells, resulting in increased proliferation, decreased apoptosis, and acquisition of a stem cell-like phenotype. (hindawi.com)
  • vivo
  • We established an optimized approach to induce high-odontogenic potential dental epithelium derived from mES cells by temporally controlling bone morphogenic protein 4 (BMP4) function and regenerated tooth-periodontium complex structures in vivo. (biomedcentral.com)
  • This procedure was designed to provide conditions resembling the in vivo microenvironment that is home for the most-primitive stem cells. (biologists.org)
  • We have combined specific culture conditions, including extracellular matrix substrata, oxygen tension, growth factors and vitamins, cell density and co-culture of cells to resemble the in vivo niche microenvironment in which the more primitive cells are expected to be found ( Watt and Hogan, 2000 ). (biologists.org)
  • Cellular
  • Additionally, these cells might yield insights into the processes underlying cellular senescence, cancer and ageing ( Zeng, 2007 ). (biologists.org)
  • The transition from a pluripotent stem cell through progressive stages of differentiation probably involves dynamic changes in the energy demand for cellular processes, depending on the needs of the individual cell types. (biologists.org)
  • MIAMI cells proliferate extensively without evidence of senescence or loss of differentiation potential and thus may represent an ideal candidate for cellular therapies of inherited or degenerative diseases. (biologists.org)
  • viability
  • We make specific reference to two assisted reproductive technologies, namely mitochondrial supplementation and somatic cell nuclear transfer, and how modulating the mitochondrial content in the oocyte influences embryo viability and the potential to generate enhanced offspring for livestock production purposes. (publish.csiro.au)
  • methylation
  • We scanned soybean and Arabidopsis seed genomes for hypomethylated regions, or DNA methylation valleys (DMVs), present in mammalian cells. (pnas.org)
  • These hypomethylated regions are similar to the DNA methylation valleys (DMVs), or canyons, found in mammalian cells. (pnas.org)
  • indefinitely
  • While the viral genome may persist in the nucleus of such cells indefinitely, it retains the ability to re-enter the lytic cycle and produce new virions if conditions in the cell become unfavorable. (scienceopen.com)
  • Mechanisms
  • ZEB2 and LMO2 drive immature T-cell lymphoblastic leukemia via distinct oncogenic mechanisms. (nih.gov)
  • Here, we focus on the mechanisms that control T cell differentiation, with a particular focus on the role of regulatory elements encoded within the genome, known as transcriptional enhancers (TEs). (frontiersin.org)
  • The efficiency and reproducibility of some reprogramming events represent unexploited avenues in which to probe mechanisms that ensure robust cell conversion. (sciencemag.org)
  • insulin
  • Diabetes mellitus, which is the result of autoimmune destruction of the insulin-producing β cells, occurs by loss of insulin-secreting capacity. (springer.com)
  • The insufficient source of insulin-producing cells (IPCs) is the major obstacle for using transplantation as diabetes treatment method. (springer.com)
  • The differentiated mESCsco express other markers of pancreatic islet cells as well as insulin. (springer.com)
  • This method exhibited higher insulin generation and further improvement in IPCs protocol that may result in an unlimited source of ES cells suitable for transplantation. (springer.com)
  • transcriptional
  • USP7 Cooperates with NOTCH1 to Drive the Oncogenic Transcriptional Program in T-Cell Leukemia. (nih.gov)
  • While it is understood that the phenotypic and functional differences between cell types are underscored by cell type-specific transcriptional profiles ( 5 , 6 ), how these unique profiles arise and are maintained is far from resolved. (frontiersin.org)
  • Transdifferentiation
  • We report that a conserved H3K27me3/me2 demethylase, JMJD-3.1, and the H3K4 methyltransferase Set1 complex cooperate to ensure invariant transdifferentiation (Td) of postmitotic Caenorhabditis elegans hindgut cells into motor neurons. (sciencemag.org)