Directed differentiation of human embryonic stem cells (hESCs) has generated much interest in the field of regenerative medicine. (ca.gov)
Upon differentiation in vitro, CD133(+)GFP(+) hESCs gave rise solely to ectoderm, as detected by expression of nestin. (ca.gov)
However, the possible differences in proliferation and differentiation capabilities among independently-derived human embryonic stem cells (hESCs) are not well known because of insufficient characterization. (biomedcentral.com)
All cell lines differentiated into three embryonic germ lineages in embryoid bodies and into neural cell lineages when cultured in neural differentiation medium. (biomedcentral.com)
In the present study, a side-by-side comparison of the ability to maintain an undifferentiated state and to self-renew under standard conditions, the ability to spontaneously differentiate into cell types of three germ layers in embryonic bodies, and directed differentiation under neural differentiation-promoting conditions was made between three NIH registered hESC lines I3, I6 and BG01V. (biomedcentral.com)
The effect of such engineered conduit on PC12 differentiation was investigated by analyzing differentiation markers Nestin and microtubule-associated protein 2 (MAP2) through immunocytochemistry and PCR-RT techniques. (yjxinhua.com)
The result revealed that such conduit could significantly induce Nestin and MAP2 gene expression in the PC12 cells and, thus, it is a viable option for effective cell differentiation and nerve regeneration. (yjxinhua.com)
In the CNS, nestin is downregulated upon differentiation and replaced by neurofilaments. (csb-bioenergetics.nl)
Transient expression of nestin has been postulated as a key step committing cells to the neural differentiation pathway. (csb-bioenergetics.nl)
The molecular events leading to human embryonic stem cell (hESC) differentiation are the subject of considerable scrutiny. (northwestern.edu)
Progenitor10
suggesting that progenitor cells in the embryonic and adult brain may share similar molecular phenotypes. (jneurosci.org)
Recent studies have reported that taurine causes increased proliferation of neural stem/progenitor neural cells (neural precursor cells, NPCs) obtained from embryonic and adult rodent brain. (nih.gov)
Expression of PRTG protein is strong in the neural tube of mouse embryos between embryonic days 7.75 and 9.5 but disappears after embryonic day 10.5 when the neural progenitor marker nestin expresses prominently. (nycu.edu.tw)
The present study tested the hypothesis that fibroblast progenitor cells express nestin, and the intermediate filament protein drives the migratory phenotype of ventricular fibroblasts. (bvsalud.org)
Transcription factor 21 (Tcf21)- and Wilms tumor 1 (WT1)-fibroblast progenitor cells identified in the epicardial/endocardial regions of the E12.5- to E13.5-day embryonic mouse heart predominantly expressed nestin. (bvsalud.org)
Thus, nestin represents a marker of embryonic Tcf21/WT1(+)-fibroblast progenitor cells. (bvsalud.org)
The neonatal rat heart contains a distinct subpopulation of nestin-immunoreactive Tcf21/WT1(+) fibroblasts refractory to cell cycle reentry, and the intermediate filament protein may preferentially facilitate ventricular fibroblast migration during physiological/pathological remodeling.NEW & NOTEWORTHY Tcf21/WT1(+)-fibroblast progenitor cells of the embryonic mouse heart predominantly express the intermediate filament protein nestin. (bvsalud.org)
Nestin is an intermediate filament protein known to play several roles, including as a marker of neural progenitor and proliferating endothelial cells. (molvis.org)
The key difference between mesenchymal markers and stem cell markers is that mesenchymal markers originate from the multipotent mesoderm derived progenitor cells while stem cell markers originate from embryonic stem cells or pluripotent stem cell surfaces. (differencebetween.com)
individual sensory progenitor cells extracted from NIH accepted L9 individual embryonic control cells had been attained in a commercial sense (Millipore, Billercia, Mother). (researchhunt.com)
Sox24
These cells possess the capability to differentiate into neuronal subtypes and exhibit high amounts of nestin and SOX2 and low amounts of March-4. (researchhunt.com)
These cells also expressed NSC markers like Nestin, Sox2, CD133 and SSEA-1, as well as early development markers of embryonic stem cells (ESCs) like BMP4, c-Myc, OCT4 and Gbx2. (ehu.eus)
The function of WNT signaling is primarily mediated by TCF7, which directly induces expression of Sox2 and Nestin. (zgmx.org.cn)
Inhibition of WNT signaling, overexpression of dominant-negative Tcf7, and silencing Tcf7, Sox2, or Nestin all resulted in drastically reduced tumor formation and substantially improved retinal integration and visual preservation in mice. (zgmx.org.cn)
Neurons6
It is currently not known whether dopamine (DA) neurons derived from human embryonic stem cells (hESCs) can survive in vivo and alleviate symptoms in models of Parkinson disease (PD). (elsevierpure.com)
Although electroporation and viral vector-mediated gene delivery techniques have been used to introduce exogenous DNA into chicken embryonic cells, transducing neurons efficiently and specifically remains challenging. (biomedcentral.com)
Importantly, many neurons generated from nestin-GFP-sorted mesencephalic precursors developed a dopaminergic phenotype in vitro. (elsevierpure.com)
Together, these findings indicate that precursor cells harvested from the embryonic ventral mesencephalon can generate dopaminergic neurons able to restore function to the chemically denervated adult striatum. (elsevierpure.com)
IUGR fetuses at embryonic day 19 (E19) had reduced nestin (progenitors), β‐III tubulin (immature neurons), Glial fibrillary acidic protein (astrocytes), and O4 (oligodendrocytes) cell lineages via immunofluorescence quantification and a 30% reduction in cortical thickness. (ucla.edu)
A potentially pre-clinical aspect of this thesis is detailed in paper №4 where I describe a robust protocol for the generation of functional mesDA neurons from human embryonic stem cells that are functional in a rat model of PD. (lu.se)
GFAP3
The most important neural markers used nowadays are the GFAP, Nestin, NeuroD antibodies and others. (wikipedia.org)
In the control retina, nestin was found principally in glial structures in the ganglion cell layer, as confirmed by nestin/GFAP immunolabeling. (molvis.org)
In addition, SV40T up-regulated the expressions of neural crest-associated markers Nestin, Pax3 and Slug, and down-regulated S100b as well as the markers of mature SCs MBP, GFAP and Olig1/2. (ehu.eus)
Markers7
METHODS: We studied a panel of putative CSC surface markers (ALDH1A1, ABCG2, CD44v7/8, CD44v10, CD133, CD271, and Nestin) in 40 established melanoma cell lines and four early-passage melanoma strains by flow cytometry. (bvsalud.org)
CONCLUSIONS: The testing of melanoma cell lines (n = 40), early-passage cell strains (n = 4), and melanoma tissues (n = 40) showed that several putative CSC markers (ALDH1A1, ABCG2, CD44v7/8, CD44v10, CD133, CD271, and Nestin) are commonly present in a large proportion of melanoma cells in vitro and in situ. (bvsalud.org)
Stemness markers are able to identify a subset of mesenchymal stem cells with high colony forming units or to identify embryonic stem cell-like cells. (differencebetween.com)
Nestin-driven GFP was detected in the mesencephalic ventricular zone, and it colocalized with specific markers for neural precursor cells. (elsevierpure.com)
These stem cells presented embryonic stem cell markers (Oct-4 and Nanog), an ectomesenchymal stem cell marker (Nestin), and mesenchymal stem cell markers (Stro-1 and CD146). (elsevierpure.com)
A set of markers (GSC, CK18, Gata4, Eomes, and Sox17) point to a mesendodermal nature of the epithelial cells with subsequent commitment to definitive endoderm (Sox17, Cdx2, nestin, and Islet1). (northwestern.edu)
Researchers from Johns Hopkins have discovered the presence of functional ion channels in human embryonic stem cells (ESCs). (arasysperfector.com)
NPCs can be differentiated from embryonic stem cells (ESCs) but also from induced pluripotent stem cells (iPSCs) (PMID: 11731782). (sanbio.nl)
Marker1
However, the co-immunoreactivity of nestin and GS, a marker of mature functional Müller cells, could be observable only from the seventh day. (molvis.org)
Progenitors2
Many of us identified progenitors by simply assaying for that expression regarding proliferating cellular atomic antigen (PCNA), N-cadherin as well as the nestin-related filament transitin, along with recently produced cells by utilizing BrdU-birthdating. (apoptosisblog.com)
All of us found that there's a progressive spatial stops involving progenitors into a highly discreet CMZ during late levels regarding embryonic improvement in between E16 as well as hatching, at about E21. (apoptosisblog.com)
Germ layers1
In contrast, CD135(+)GFP(+) hESCs gave rise to tissues representing all three embryonic germ layers, and were virtually indistinguishable from CD135(-)-derived tissues. (ca.gov)
Neuronal1
Accordingly, prior studies have conducted neuronal transduction of embryonic chicken brains using recombinant avian adeno-associated viruses (rAAAVs) and the ubiquitous RSV promoter [ 6 ]. (biomedcentral.com)
NSCs4
Trim71 deletion in prenatal mouse NSCs (Nestin-Trim71fl/fl and Emx-Trim71fl/fl), or knock-in of a human congenital hydrocephalus-associated missense mutation that disrupts the regulation of target RNAs (Trim71R595H/+), causes severe cortical thinning and associated ventriculomegaly due to defective NSC self-renewal without altering ependymal cilia- driven cerebrospinal fluid flow. (yale.edu)
The recent discovery of induced pluripotent stem cells (iPSCs) not only overcomes the ethical and logistical issues associated with human embryonic stem cells, but also provides a flexible platform for generating various differentiated cell types from diseased individuals.iPSC-derived NSCs are a potentially valuable source of in vitro models for complex, polygenic human diseases, and are potentially useful for drug discovery and cell-based therapy applications [2] . (ixcellsbiotech.com)
Lentiviral short-hairpin RNA (shRNA)-mediated nestin depletion inhibited the in vitro migratory response of NNVFbs as the wound radius was significantly larger compared with NNVFbs infected with the empty lentivirus. (bvsalud.org)
Pax62
Finally, Tbr2 was examined in the context of additional TFs that, together, define a TF cascade in embryonic neocortical neurogenesis (Pax6 → Ngn2 → Tbr2 → NeuroD → Tbr1). (jneurosci.org)
Nestin, Pax6 and Sox1 (Figure 1 and Figure 2), with the purity higher than 97% (Figure 3). (ixcellsbiotech.com)
Antibody2
Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66259-1-Ig (Nestin Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. (ptglab.com)
Human Antibody Laboratories manufactures the human nestin antbody reagents distributed by Genprice. (csb-bioenergetics.nl)
Precursor cells1
To visualize and isolate mesencephalic precursor cells from a mixed population, we used transgenic mice and rats carrying green fluorescent protein (GFP) cDNA under the control of the nestin enhancer. (elsevierpure.com)
Retina2
Nestin was also observed in some vascular cells, as demonstrated by the co-immunoreactivity of the protein with vWF in the choroid and retina. (molvis.org)
As a result, the purpose of these studies ended up being to characterize the formation and patterning from the CMZ from the embryonic chicken retina. (apoptosisblog.com)
Hematopoietic1
Nestin expression has also been observed in pancreatic hematopoietic stem cell populations. (csb-bioenergetics.nl)
Neurogenesis1
These results suggest that Tbr2+ IPCs play a major role in the regulation of adult hippocampal neurogenesis, and that a similar transcriptional program controls neurogenesis in adult SGZ as in embryonic cerebral cortex. (jneurosci.org)
Protein6
Nestin, also named as NES and Nbla00170, is a class VI intermediate filament protein expressed in stem cells of the central nervous system (CNS) but not in mature CNS cells. (ptglab.com)
The intermediate filament protein nestin is implicated in cell migration and expression identified in a subpopulation of scar-derived myofibroblasts. (bvsalud.org)
Nuclear Tcf21/WT1 expression persisted in adult rat ventricular fibroblasts, whereas nestin protein levels were downregulated. (bvsalud.org)
Description: Nestin is a class VI intermediate filament protein expressed predominantly in stem cells of the neural tube but absent from virtually all differentiated CNS cells. (csb-bioenergetics.nl)
Description: Nestin is a type VI intermediate filament (IF) protein that in humans is encoded by the NES gene. (csb-bioenergetics.nl)
Figure 1), brain lipid-binding protein (BLBP), and nestin (PMID: 21549330). (sanbio.nl)
Human6
Subpopulations of human embryonic stem cells with distinct tissue-specific fates can be selected from pluripotent cultures. (ca.gov)
66259-1-Ig targets Nestin in WB, FC, ELISA applications and shows reactivity with human, mouse, rat samples. (ptglab.com)
Human embryonic stem cells (hESCs) possess the ability to self-renew in an undifferentiated state in culture while retaining the ability to differentiate into all of the cell types in the human body. (biomedcentral.com)
In the present study, we performed a comparative study of the ubiquitous CMV promoter and three neuron-specific promoters, chicken Ca2+/calmodulin-dependent kinase (cCaMKII), chicken Nestin (cNestin), and human synapsin I. We explored the possibility of manipulating gene expression in chicken embryonic brain cells using in ovo electroporation with the selected promoters. (biomedcentral.com)
The Human Nestin Antbody reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. (csb-bioenergetics.nl)
From embryonic development to tissue regeneration and disease progression, the human body is continuously subject to mechanical stresses. (5dok.org)
Glial1
Our results confirmed the presence of nestin in glial (e.g., astrocytes), reactive Müller, and endothelial cells. (molvis.org)
Ventricular2
Nuclear Tcf21/WT1 staining was identified in neonatal rat ventricular fibroblasts (NNVFbs), and a subpopulation coexpressed nestin. (bvsalud.org)
A subpopulation of Tcf21/WT1(+)-neonatal rat ventricular fibroblasts express nestin and are refractory to selective stimuli influencing cell cycle reentry. (bvsalud.org)
Proteins1
Distribution and expression of nestin in mitotically active cells suggests it plays a role in regulation of the assembly and disassembly of intermediate filaments, which, together with other structural proteins, participate in remodeling of the cell. (csb-bioenergetics.nl)
Cells5
hNPCs obtained from 3 fetal brains (14-15 weeks of gestation) were cultured and expanded as neurospheres, which contained 76.3% nestin-positive cells. (nih.gov)
A unique and essential property of embryonic stem cells is the ability to self-renew and differentiate into multiple cell lineages. (biomedcentral.com)
Finally, nestin-GFP + cells were transplanted into the striatum of a rat model of Parkinson's disease. (elsevierpure.com)
Tumor formation constitutes a major obstacle to the clinical application of embryonic stem cell-derived (ESC-derived) cells. (zgmx.org.cn)
Conversely, targeted expression of Dkk1 in dental epithelial cells leads to the formation of dysfunctional enamel knots and subsequent tooth defects during embryonic development. (tamu.edu)
Gene1
In ovo electroporation offers an easier and more efficient way to manipulate gene expression during embryonic development, and this technique will be useful for neuron-targeted transgene expression. (biomedcentral.com)
Exhibit1
Strikingly, Trim71R595H/R595H mice exhibit anencephaly and embryonic lethality. (yale.edu)
Phenotype2
Nestin-expressing NNVFbs exhibited a unique phenotype as the subpopulation was refractory to cell cycle reentry in response to selective stimuli. (bvsalud.org)
Nestin(-)- and nestin(+)-scar-derived rat myofibroblasts plated in Matrigel unmasked a migratory phenotype characterized by the de novo formation of lumen-like structures. (bvsalud.org)
Chicken1
By using in ovo electroporation of plasmid DNA into embryonic chicken eyes, Chen et al. (biomedcentral.com)
Expression2
Two days after the laser impacts, the nestin expression extended to numerous radial processes at the site of the impacts. (molvis.org)
and (5) a dramatic change in Osx and nestin expression. (tamu.edu)
Structures1
The elongated membrane projections emanating from scar myofibroblasts delineating the boundary of lumen-like structures expressed nestin. (bvsalud.org)
Positive1
In addition, Noggin diminished the occurrence of a fibroblast-like Nestin-positive precursor population that differentiated into myocytes. (elsevierpure.com)
Role2
In this study, we examined the role of nestin in this process. (molvis.org)
The role of nestin was examined using angiography, western blot from the second to the 14th day after laser impacts, and intraocular injection of nestin siRNA. (molvis.org)
High1
Nestin immunolabeling remained high from the third to the seventh day but appeared reduced on the 14th day. (molvis.org)