• We showed that the extracted criteria could be used to evaluate the differences between individual embryos. (nature.com)
  • Advances in technology facilitated noninvasive measurement of amino acid uptake/output into the spent culture medium of individual embryos, and products of embryo metabolism have been quantified and used in attempts to identify healthy embryos. (parents-life.com)
  • The use of amino acid turnover to predict the developmental competence of individual embryos is based on the premise that metabolism is intrinsic to early embryo health and that the embryonic metabolome is immediately perturbed when embryos are stressed. (parents-life.com)
  • The use of a mesh system allows identification of individual embryos by position, and control of their orientation during culture and preparation for light and electron microscopy. (sciencegate.app)
  • We are also interested in how variations in DNA methylation come about in oocytes and whether we can use this variation as a marker for oocyte quality and embryo potential. (babraham.ac.uk)
  • Here, we have utilized single-cell methylome and transcriptome sequencing (scM&T-seq) to quantify both mRNA expression and DNA methylation in oocytes and a developmental series of human embryos at single-cell resolution. (babraham.ac.uk)
  • Genome-wide assessment of DNA methylation alterations induced by superovulation, sexual immaturity and in vitro follicle growth in mouse blastocysts. (babraham.ac.uk)
  • Here we report genome-wide composition, patterning, and stage-specific dynamics of DNA methylation in pre-implantation rhesus monkey embryos as well as male and female gametes studied using an optimized tagmentation-based whole-genome bisulfite sequencing method. (nature.com)
  • We show that upon fertilization, both paternal and maternal genomes undergo active DNA demethylation, and genome-wide de novo DNA methylation is also initiated in the same period. (nature.com)
  • By the 8-cell stage, remethylation becomes more pronounced than demethylation, resulting in an increase in global DNA methylation. (nature.com)
  • Monkeys have served as one of the most valuable models for understanding DNA methylation dynamics during early embryogenesis in human due to their similarities in genetics and early embryonic development 17 , 18 . (nature.com)
  • Non-CpG methylation can however be observed in embryonic stem cells, and has also been indicated in neural development. (wikipedia.org)
  • By contrast, the genome of most plants, invertebrates, fungi, or protists show "mosaic" methylation patterns, where only specific genomic elements are targeted, and they are characterized by the alternation of methylated and unmethylated domains. (wikipedia.org)
  • High CpG methylation in mammalian genomes has an evolutionary cost because it increases the frequency of spontaneous mutations. (wikipedia.org)
  • Howlett S.K., Reik W., Methylation levels of maternal , and paternal genomes during preimplantation development. (biologie-journal.org)
  • In this review, we take a wide view of this problem by analyzing the strategies involved in setting up normal DNA methylation patterns and understanding how this stable epigenetic mark works to prevent gene activation during development. (aacrjournals.org)
  • Then, at the time of implantation, there is a wave of de novo methylation that modifies almost all CpGs in the genome except for CpG islands that are protected ( 2 ). (aacrjournals.org)
  • Thus, maternal UHRF1 regulates the proper cytoplasmic architecture and function of oocytes and preimplantation embryos, likely through a mechanism unrelated to DNA methylation. (life-science-alliance.org)
  • At blastocyst stage, the segregation of the three primordial lineages is accompanied by establishment of differential patterns of DNA methylation and post-translational modifications of histones, such as H3K27me3. (sciencegate.app)
  • The developing embryo, from which pluripotent stem cells originate, undergoes a series of dynamic metabolic transitions synchronized to its molecular development. (conditionmed.org)
  • Revealing cell populations catching the early stages of the human embryo development in naïve pluripotent stem cells. (axonmedchem.com)
  • A model of the post-implantation human embryo derived from pluripotent stem cells. (caltech.edu)
  • Dynamic status of lysosomal cathepsin in bovine oocytes and preimplantation embryos. (researchmap.jp)
  • However, UHRF1 is predominantly localized in the cytoplasm of mouse oocytes and preimplantation embryos, where it may play a role unrelated to the nuclear function. (life-science-alliance.org)
  • however, no differences were noted in the expression of zygotic genome activation-related genes. (cqmu.edu.cn)
  • These were identified to have the capacity to undergo zygotic genome activation and blastocyst development, and were compared with embryos which are metabolically active but are destined to undergo cleavage arrest. (parents-life.com)
  • We fully characterize embryonic genome activation and maternal transcript degradation and map key epigenetic reprogramming events in developmentally high-quality embryos. (babraham.ac.uk)
  • The improvement of laboratory standards and modifications of the culture media helped to achieve high-quality embryos. (parents-life.com)
  • This protein also forms a core subunit of the nucleosome remodeling and histone deacetylation (NURD) complex that epigenetically regulates embryonic stem cell differentiation. (cancerindex.org)
  • Thus, we investigated male mouse (m) PRL protein and mRNA expression in spermatozoa at various differentiation stages in the testes. (go.jp)
  • Removal of sperm tail using trypsin and pre-activation of oocyte facilitates intracytoplasmic sperm injection in mice and rats. (stanford.edu)
  • After intracytoplasmic sperm injection (ICSI), 48 embryos were evaluated on day 3 of their development, according to their cell number. (who.int)
  • Adenot P.G., Mercier Y., Renard J.P., Thompson E.M., Differential H4 acetylation of paternal, maternal chromatin precedes DNA replication, and differential transcriptional activity in pronuclei of 1-cell mouse embryos. (biologie-journal.org)
  • Haaf T., The battle of the sexes after fertilization: behaviour of paternal and maternal chromosomes in the early mammalian embryo. (biologie-journal.org)
  • These studies show that maternal novel method that enables sensitive and assessed in plasma taken at 1-16 weeks exposure and diet during the early stages targeted deep DNAm analysis in minute of pregnancy, and global DNAm of of pregnancy is associated with changes amounts of DNA present in body fluids white blood cel s from their infants was in epigenome patterns of infants. (who.int)
  • At the 8-cell stage, the embryo becomes compact, and the cells form a spherical mass called a morula. (nature.com)
  • The space inside the embryo spreads, and the morula becomes a blastocyst. (nature.com)
  • The first marked increase in the metabolism of glucose by ovine embryos was detected in compact morula stage, but there was no significant increase in the oxidation of glucose after the morula stage. (biomedcentral.com)
  • In contrast to the weak but definite nuclear deposition of Asf1a, Asf1b disappeared from embryonic nuclei at morula and blastocyst stages. (omicsdi.org)
  • 2- to 4-cell and morula- to blastocyst-stage mouse embryos were cultured for 1 h in tritiated leucine at two specific activities and their subsequent development followed in vitro and in vivo (after transfer to recipients), respectively. (sciencegate.app)
  • Giraldo A.M., Hylan D.A., Ballard C.B., Purpera M.N., Vaught T.D., Lynn J.W., Godke R.A., Bondioli K.R., Effect of epigenetic modifications of donor somatic cells on the subsequent chromatin remodeling of cloned bovine embryos. (biologie-journal.org)
  • Here, we consider the metabolism of the early embryo through development, and look at the nutrient milieu within the developing stem cell niche. (conditionmed.org)
  • It is only through understanding embryonic metabolism and development that we can derive and maintain different in vitro stem cell states for disease modeling and therapies. (conditionmed.org)
  • Efficient generation of embryonic stem cells from single blastomeres of cryopreserved mouse embryos in the presence of signalling modulators. (axonmedchem.com)
  • The related coactivator complexes SAGA and ATAC control embryonic stem cell self-renewal through acetyltransferase-independent mechanisms. (axonmedchem.com)
  • Generation of Stem Cell-Based Mouse Embryo-Like Structures. (caltech.edu)
  • Mouse embryo model derived exclusively from embryonic stem cells undergoes neurulation and heart development. (caltech.edu)
  • Stem-cell-based human and mouse embryo models. (caltech.edu)
  • Stem cell-derived synthetic embryos self-assemble by exploiting cadherin codes and cortical tension. (caltech.edu)
  • Machine learning-assisted high-content analysis of pluripotent stem cell-derived embryos in vitro. (caltech.edu)
  • In mouse, only epiblast cells can be directly converted into cultured pluripotent embryonic stem cells, capable of forming all adult cell types. (bioone.org)
  • However, it is disputed whether TBLCs are 'true' totipotent stem cells equivalent to in vivo two-cell stage embryos. (sciencegate.app)
  • During pre-implantation stages of mammalian development, maternally stored material promotes both the erasure of the sperm and oocyte epigenetic profiles and is responsible for concomitant genome activation. (babraham.ac.uk)
  • Although the genome-wide DNA demethylation is believed to be a hallmark of mammalian embryogenesis, previous study also indicated that the somatic form of dnmt1 ( dnmt1s ) is actually expressed at each stage of pre-implantation embryos and plays a role in the maintenance of DNA imprinting 8 . (nature.com)
  • Abdalla H., Yoshizawa Y., Hochi S., Active demethylation of paternal genome in mammalian zygotes. (biologie-journal.org)
  • Bellier S., Chastant S., Adenot P., Vincent M., Renard J.P., Bensaude O., Nuclear translocation , and carboxyl-terminal domain phosphorylation of RNA polymerase II delineate the two phases of zygotic gene activation in mammalian embryos. (biologie-journal.org)
  • Fulka H., St John J.C., Fulka J., Hozak P., Chromatin in early mammalian embryos: achieving the pluripotent state. (biologie-journal.org)
  • We examine how epigenetic states are set up in oocytes - or egg cells - and influence gene expression in the embryo. (babraham.ac.uk)
  • Single-cell multi-omic analysis profiles defective genome activation and epigenetic reprogramming associated with human pre-implantation embryo arrest. (babraham.ac.uk)
  • By comparing these signatures with early embryos that have undergone spontaneous cleavage-stage arrest, as determined by time-lapse imaging, we identify embryos that fail to appropriately activate their genomes or undergo epigenetic reprogramming. (babraham.ac.uk)
  • Exposure to environmental toxins during embryonic development may lead to epigenetic changes that influence disease risk in childhood and later life. (who.int)
  • In 1-cell and 2-cell embryos Dnmt1s is derived from the oocyte, whereas from the 2-cell stage onward the embryo starts to synthesize its own Dnmt1s 8 . (nature.com)
  • Testing this method in rats yielded similar results, although the oocyte activation method was different. (stanford.edu)
  • We herein report that oocyte-specific Uhrf1 KO results in impaired chromosome segregation, abnormal cleavage division, and preimplantation lethality of derived embryos. (life-science-alliance.org)
  • Unlike in rodents, X chromosome inactivation is not observed during monkey pre-implantation development. (nature.com)
  • However, robust and large-scale genome-wide reprogramming of DNA methylome occurs during two critical developmental processes: (1) development of primordial germ cells and (2) pre-implantation embryogenesis. (nature.com)
  • Furthermore, due to ethical and legal concerns, very limited techniques can be applied to human embryos to validate some of significant conclusions drawn from descriptive studies regarding human embryonic development. (nature.com)
  • Thus, embryo development is highly dynamic. (nature.com)
  • Surgical embryo recovery was performed on Days 4 and 6 after onset of estrus (Day 0) and recovered embryos were subjected to comparative metabolism studies with in vitro derived embryos at the same stage of development. (biomedcentral.com)
  • In addition, pyruvate (0.33 mmol/L) and lactate (3.3 mmol/L) together were beneficial to early embryonic development in sheep[ 3 ]. (biomedcentral.com)
  • Western blotting and qRT-PCR were used to analyse the expression of TOP2A and proteins involved in trophoblast functions, the FOXO signalling pathway, and the development of pre-implantation embryos. (cqmu.edu.cn)
  • Additionally, TOP2A inhibition resulted in impaired development of pre-implantation embryos in mice, which could be attributed to excessive oxidative stress. (cqmu.edu.cn)
  • Braude P., Bolton V., Moore S., Human gene expression first occurs between the four- , and eight-cell stages of preimplantation development. (biologie-journal.org)
  • Christians E., Rao V.H., Renard J.P., Sequential acquisition of transcriptional control during early embryonic development in the rabbit. (biologie-journal.org)
  • Modification of embryo transfer techniques and development of softer catheters were steps forward toward better results. (parents-life.com)
  • The development of a blastocyst encouraged clinicians to transfer one embryo, which satisfied the request of the regulatory bodies to avoid multiple pregnancies. (parents-life.com)
  • Distinct role of histone chaperone Asf1a and Asf1b during fertilization and pre-implantation embryonic development in mice. (omicsdi.org)
  • METHODS:We analyzed the dynamics of histone chaperone Asf1a and Asf1b in oocytes and pre-implantation embryos in mice by immunofluorescence and real-time quantitative PCR, and further investigated the role of Asf1a and Asf1b during fertilization and pre-implantation development by specific Morpholino oligos-mediated knock down approach. (omicsdi.org)
  • During early development in vivo and in vitro, each preimplantation embryo will utilize numerous substrates from its immediate environment: oxygen for respiration, sugars, energy sources such as glucose and proteins/amino acids. (parents-life.com)
  • Development of technologies to measure embryo metabolism led to the science of "embryo metabolomics," defined as "the systematic analysis of the inventory of metabolites - as small molecule biomarkers - that represent the functional phenotype at the cellular level. (parents-life.com)
  • This process gets rid of unneeded cells and is particularly important for "sculpting" tissue and organ structure during development of the embryo (or larval metamorphosis in insects), but may occur at any time even in adult cells when a tissue needs to be remodeled. (agemed.org)
  • Poor in vitro embryo development is a major obstacle in porcine assisted reproduction. (mdpi.com)
  • During development, these will form extraembryonic and embryonic tissues, respectively. (bioone.org)
  • In this study, we have analysed the dynamics of H3K27me3 at pericentromeric heterochromatin (PCH) during development of the mouse blastocyst, in comparison with cultured embryonic cells. (sciencegate.app)
  • To facilitate a quantitative morphological analysis of early mouse development under controlled conditions, a perfusion culture system capable of supporting embryogenesis to blastocyst stage has been developed. (sciencegate.app)
  • Through the definition of a coordinate system based on mesh structure and the development of a special sectioning procedure, sections can be localized within the intact embryo and three-dimensional coordinates given to any element of embryo volume. (sciencegate.app)
  • In vivo and in organized cells, and proper symmetry are healthy individuals, macrophages can characteristics of higher-quality embryos, which phagocytize DNA that has been passively point to healthy development and higher rates of released into the blood from apoptotic or necrotic implantation. (who.int)
  • Adenot P.G., Szollosi M.S., Geze M., Renard J.P., Debey P., Dynamics of paternal chromatin changes in live one-cell mouse embryo after natural fertilization. (biologie-journal.org)
  • Huang J.C., Lei Z.L., Shi L.H., Miao Y.L., Yang J.W., Ouyang Y.C., Sun Q.Y., Chen D.Y., Comparison of histone modifications in in vivo and in vitro fertilization mouse embryos. (biologie-journal.org)
  • Antimüllerian hormone (AMH) and age as predictors of preimplantation genetic testing for aneuploidies (PGT-A) cycle outcomes and blastocyst quality on day 5 in women undergoing in vitro fertilization (IVF). (ivi-rmainnovation.com)
  • SUMOylation of linker histone H1 drives chromatin condensation and restriction of embryonic cell fate identity. (axonmedchem.com)
  • Rieger[ 4 ] inferred that the pre-implantation bovine embryo undergoes an intensified period of cellular synthetic processes which require appropriate substrates for production of energy and reducing equivalents in the form of nicotinamide-adenine dinucleotide phosphate. (biomedcentral.com)
  • Potential of preimplantation genomic selection using the blastomere separation technique in bovine in vitro fertilized embryos. (researchmap.jp)
  • In vitro fertilized (IVF) embryos were cultured in the presence of dimethyl sulfoxide or inhibitors of MAP2K (0.4 μM PD0325901) and GSK3 (3 μM CHIR99021) from the zygote (Day 1) stage. (bioone.org)
  • The synchronization and ovulatory responses of Sangsari cross bred ewes and metabolism of energy substrates in 8-cell stage embryos to hatched blastocysts stage produced in vitro or in vivo were investigated. (biomedcentral.com)
  • While the pattern of oxidation was similar among in vitro and in vivo derived embryos, a low pyruvate to lactate ratio was the preferred substrate of embryos derived in vitro . (biomedcentral.com)
  • The production of CO2 from glucose by sheep embryos has been determined[ 3 ] and results have indicated that little glucose is utilized by the embryos of sheep and that blastocysts derived from oocytes, matured and fertilized in vitro , oxidized glucose at a lower rate than those developed in vivo . (biomedcentral.com)
  • But, as in vitro culture conditions are different from those in vivo, cleaved Day-3 embryos may fail to reach the blastocyst stage at in vitro culture media. (parents-life.com)
  • The same embryo may survive and proceed normally at in vivo conditions. (parents-life.com)
  • Evidence that interferon-tau secreted from Day-7 embryo in vivo generates anti-inflammatory immune response in the bovine uterus. (researchmap.jp)
  • Currently, the ability to culture in vitro totipotent cells possessing molecular and functional features like those of an early embryo in vivo has been a challenge. (sciencegate.app)
  • This study underscores the subtle differences between in vitro derived TBLCs and in vivo mouse early developmental cell stages at the single-cell transcriptomic level. (sciencegate.app)
  • After adjusting for confounding factors, the risk of preterm birth was significantly greater after blastocyst stage than after cleaved-stage transfer. (parents-life.com)
  • Nevertheless, on the basis of the turnover of three to five key amino acids, morphologically similar cleavage stage human embryos which are metabolically "quiet" were identified. (parents-life.com)
  • Interestingly, amino acid turnover by early cleavage embryos appears to be linked to embryo genetic health. (parents-life.com)
  • Preliminary trials of in vitro amino acid profiling suggested that this strategy might be used to identify cleavage stage embryos with high implantation potential. (parents-life.com)
  • Understanding the association between Cell-free DNA levels in embryo CM and the quality of embryo cleavage could help improve the quality of IVF techniques. (who.int)
  • This prospective study was conducted with 96 spent CM from patients undergoing IVF cycle, in order to determine relationships of Cell-free DNA levels in embryo CM with embryo cleavage quality on day 3. (who.int)
  • We conclude that cel -free DNA levels in CM might be associated with delayed embryo cleavage. (who.int)
  • The developmental potential within pluripotent cells in the canonical model is restricted to embryonic tissues, whereas totipotent cells can differentiate into both embryonic and extraembryonic tissues. (sciencegate.app)
  • The TBLCs exhibited totipotency transcriptionally and acquired expanded developmental potential with the ability to yield various embryonic and extraembryonic tissues that may be employed as novel mouse developmental cell models. (sciencegate.app)
  • In mouse, a unidirectional demethylation process from the zygote stage to blastocyst stage is observed using either reduced representation bisulfite sequencing (RRBS) or single-base resolution whole-genome bisulfite sequencing (WGBS) method. (nature.com)
  • RESULTS:Immunofluorescence with specific antibodies revealed that both Asf1a and Asf1b were deposited in the nuclei of fully grown oocytes, accumulated abundantly in zygote and 2-cell embryonic nuclei, but turned low at 4-cell stage embryos. (omicsdi.org)
  • Drosophila embryos are easily amenable to imaging because they are more transparent than the embryos of other model organisms, such as mice. (nature.com)
  • Amino acid profiling has been extensively tested as a valid clinical diagnostic test for embryo selection in animal species, including mice, cows and pigs, as well as humans. (parents-life.com)
  • As a unique functional test of these iPSCs, we injected them into the pre-implantation embryos of another non-human species, rhesus macaques (Macaca mulatta). (stanford.edu)
  • Metabolic profiles of developmentally competent, frozen-thawed human embryos were also consistent with those of fresh embryos, and metabolic profiles could be used to identify frozen-thawed embryos with the potential to develop to the blastocyst stage in vitro. (parents-life.com)
  • To analyse the time-series 3D microscopic images of developing embryos with fluorescently labelled nuclei, these studies used image segmentation. (nature.com)
  • Promoters of genes associated with oxidative phosphorylation are preferentially remethylated at the 8-cell stage, suggesting that this mode of energy metabolism may not be favored. (nature.com)
  • The embryo also secretes many waste products of metabolism into its immediate environment. (parents-life.com)
  • Embryo oxygen consumption by respirometry has also been used to quantify embryo metabolism. (parents-life.com)
  • For time-lapse observation of early-stage D rosophila embryos, Keller et al. (nature.com)
  • We show that this histone modification is first enriched at PCH in the whole embryo and evolves into a diffuse distribution in epiblast during its specification and maturation. (sciencegate.app)
  • Moreover, the dynamic of H3K27me3 at PCH during in vitro conversion from naïve to primed pluripotent state and during ESCs derivation suggests that the mechanisms underlying the control of this histone mark at PCH are different in embryo and in vitro. (sciencegate.app)
  • Moreover, the effect of TOP2A inhibitor on embryos was determined by immunofluorescence and mitochondrial-related dyes. (cqmu.edu.cn)
  • Our results indicate that a failure to successfully accomplish these essential milestones impedes the developmental potential of pre-implantation embryos and is likely to have important implications, similar to aneuploidy, for the success of assisted reproductive cycles. (babraham.ac.uk)
  • They studied 128 proteins, called trans-acting factors , which are known to regulate gene expression by binding to regulatory regions within the genome. (pharmaceuticalintelligence.com)
  • Francastel C., Magis W., Groudine M., Nuclear relocation of a transactivator subunit precedes target gene activation. (biologie-journal.org)
  • Ectopic expression of gene BCL2 enhances the survival and proliferation of chimpanzee and pig-tailed macaque iPSCs within the pre-implantation embryo, although the identity and long-term contribution of the transplanted cells warrants further investigation. (stanford.edu)
  • 100.000 cCREs in total), dispersed within the genome and present in cis-regulatory regions of ~81% of human genes, as calculated following gene enrichment analysis. (mdpi.com)
  • Mosaic results after preimplantation genetic testing for aneuploidy may be accompanied by changes in global gene expression. (ivi-rmainnovation.com)
  • Using QCANet, we were able to extract several quantitative criteria of embryogenesis from 11 early mouse embryos. (nature.com)
  • Quantitative evaluation of tissue-processing procedures has permitted selection of conditions which reduce changes in linear dimensions to −1·6 ± 1·8 % in two-cell embryos. (sciencegate.app)
  • Day 2 and day 3 CM corresponding to each one of the embryos was analyzed, by quantitative PCR, for estimation of Cell-free DNA levels. (who.int)
  • Superovulation ensures recovery of multiple embryos during one estrous cycle, thus allowing us to maximally exploit the female germ pool. (biomedcentral.com)
  • However, probably due to technological limitations, no study has yet revealed genome-wide DNA remethylation during early embryogenesis. (nature.com)
  • In early embryos, cells are loosely connected to each other. (nature.com)
  • To address this question, single-cell RNA sequencing was applied to TBLCs and cells from early mouse embryonic developmental stages and the data were integrated using canonical correlation analyses. (sciencegate.app)
  • Blastocyst culture provides some theoretical advantages and disadvantages, while it promotes embryo self-selection, it also exposes those embryos to possible harm due to the in vitro environment. (parents-life.com)
  • These results strongly suggest the possibility of DNA remethylation during pre-implantation embryogenesis. (nature.com)
  • Mares were killed 7.5-8.5 days after transfer and the uterus and oviducts flushed for embryo recovery. (bioone.org)
  • hence CpG dinucleotides steadily deaminate to TpG dinucleotides, which is evidenced by the under-representation of CpG dinucleotides in the human genome (they occur at only 21% of the expected frequency). (wikipedia.org)
  • Excluding repeated sequences, there are around 25,000 CpG islands in the human genome, 75% of which being less than 850bp long. (wikipedia.org)
  • Microarray profiling using the Affymetrix GeneChip Human Genome U133 plus 2.0 arrays was performed to comprehensively determine global changes in transcript levels in bronchial epithelial cells following elastase treatment. (refine.bio)
  • To do so, they coupled findings from 238 DNA-protein-binding experiments performed by the ENCODE project - a massive, multiyear international effort to identify the functional elements of the human genome - with a laboratory-based technique to identify binding patterns among the proteins themselves. (pharmaceuticalintelligence.com)
  • Human endogenous retroviruses (HERVs) are a family of endogenous retroviruses that comprise the ~8.93% of the human genome sequence, with a high proportion being human specific. (mdpi.com)
  • Human Genome Variation. (elsevierpure.com)
  • Human embryo polarization requires PLC signaling to mediate trophectoderm specification. (caltech.edu)
  • Amino acid turnover in spent embryo culture media has been measured non-invasively using high performance liquid chromatography (HPLC), gas chromatography and mass spectrometry, nuclear magnetic resonance spectroscopy and Raman near-infrared spectroscopy. (parents-life.com)
  • 2019). Akin to the dynamic nutrient requirements of the developing embryo, discrete in vitro cell states have distinct metabolic profiles (Zhou et al. (conditionmed.org)
  • The association of metabolic profiles of certain amino acids (particularly asparagine, glycine and leucine) with embryo viability is based on a variety of complex interactions that involve energy production, mitochondrial function, regulation of pH and osmolarity. (parents-life.com)
  • Genome organisation and nuclear architecture. (biologie-journal.org)
  • Nrf2 stabilization and increase in its half-life even to 200 min [ 9 ] allows nuclear translocation and activation of transcription of cytoprotective genes (Fig. 1 ). (springer.com)
  • Reply of the Authors: Recurrent implantation failure: reality or a statistical mirage? (ivi-rmainnovation.com)
  • We showed that QCANet can be applied not only to developing mouse embryos but also to developing embryos of two other model species. (nature.com)
  • An in vitro TOP2A inhibition model was established by culturing mouse embryos at the two-cell stage in a medium containing PluriSIn2, a TOP2A inhibitor. (cqmu.edu.cn)
  • In the mouse, a transporting epithelium is established around the 8-16-cell stage through a process known as compaction where cell definition is lost and the outer cells of the embryo form tight junctions, giving rise to the blastocyst. (conditionmed.org)
  • Aoki F., Worrad D.M., Schultz R.M., Regulation of transcriptional activity during the first , and second cell cycles in the preimplantation mouse embryo. (biologie-journal.org)
  • Bouniol C., Nguyen E., Debey P., Endogenous transcription occurs at the 1-cell stage in the mouse embryo. (biologie-journal.org)
  • Remarkably, a subpopulation within the TBLCs population expressed a high level of the totipotent-related genes Zscan4s and displayed transcriptomic features similar to mouse two-cell stage embryonic cells. (sciencegate.app)
  • The turnover of these substrates and products has been measured in embryos themselves and in their culture environment. (parents-life.com)
  • Chimpanzee and pig-tailed macaque iPSCs: Improved culture and generation of primate cross-species embryos. (stanford.edu)
  • At the organismal level, evolutionary distance is defined by genome-wide sequence homology between species. (frontiersin.org)
  • A molecular program that causes the interspecific barrier might be independent of genome-wide evolution. (frontiersin.org)