Embryonic fibroblasts embryonic cell. Medical search. Frequent questions

Serotoninergic and melatoninergic systems are expressed in mouse embryonic fibroblasts NIH3T3 cells. (nel.edu)
Liu Y, Meng F, Wu L, Zhou J. Serotoninergic and melatoninergic systems are expressed in mouse embryonic fibroblasts NIH3T3 cells. (nel.edu)

Swiss Mouse Embryo Fibroblast Cells with MitoTracker Red CMXRos, Alexa Fluor 488, and DAPI - A log phase culture of embryonic Swiss mouse fibroblast cells was stained with MitoTracker Red CMXRos, Alexa Fluor 488 conjugated to phalloidin, and DAPI, which target the intracellular mitochondrial network, cytoskeletal actin filaments, and nuclei, respectively. (fsu.edu)

Cellular senescence is a fundamental cellular program that is activated after a finite number of cell divisions and operates to avoid further cell proliferation. (plos.org)
Fibroblast growth factors (FGFs) are key regulators of cell proliferation, tumor-induced angiogenesis, and migration. (rcsb.org)
It was also shown to act on preosteoblasts - in the form of an increased proliferation - after binding to fibroblast growth factor receptor 1 and activating phosphoinositide 3-kinase . (wikidoc.org)
During cell growth and proliferation, ubiquitin plays an outsized role in promoting progression through the cell cycle. (intechopen.com)
Notably, the G1/S boundary represents a major barrier to cell proliferation and is universally dysfunctional in cancer cells, allowing for the unbridled proliferation observed in malignancy. (intechopen.com)
First, we found that GS-002 significantly inhibited cell proliferation and induced cell apoptosis in dose-dependent manners. (hindawi.com)
ATF-3 induces DNA synthesis and expression of cyclin D1 in hepatocytes [ 21 ] and is involved in serum-induced cell proliferation as a target gene of c-myc [ 10 ]. (hindawi.com)
the end result of these defects is genomic instability, decreased cell proliferation, and premature cell senescence and death. (medscape.com)
However, potentially erroneous characterization of cytotoxicity and cell proliferation can arise if verification of baseline interaction of test article with method employed is neglected. (cdc.gov)
Non-tumorigenic Beas-2B cells were treated with graded concentrations of benzo[a]pyrene (B[a]P) for 24 and 48h prior to cytotoxicity and proliferation assessment with commonly used MTT, MTS, WST1, and Alamar Blue assays. (cdc.gov)

Mouse Embryonic Fibroblasts (MEFs) are a type of fibroblast prepared from mouse embryo. (wikipedia.org)
MEFs show a spindle shape when cultured in vitro, a typical feature of fibroblasts. (wikipedia.org)
Nevertheless, researchers can use several strategies, like virus infection or repeated transmission to immortalize MEF cells, which can let MEFs grown indefinitely in spite of some changes in characters. (wikipedia.org)
MEFs are widely used in life science researches, especially in stem cell biology. (wikipedia.org)
To transmit MEFs, researches should use trypsin to digest the cells (making them detach from the surface) and transmit 1/5 cells digested into a new dish. (wikipedia.org)
MEFs treated by mitomycin or gamma rays (such treatment makes MEF stop mitosis) are widely used as feeder in embryonic stem cell culture because they can mimic the microenvironment in embryo. (wikipedia.org)
In 2006, Shinya Yamanaka reprogrammed MEFs into iPSCs by introducing 4 factors, which is remarkable in the development of stem cell biology. (wikipedia.org)
Here we show that in young proliferating embryonic fibroblasts (MEFs) the Polycomb Repressive Complex 2 (PRC2) member EZH2 together with PRC1 members BMI1 and M33 are strongly expressed and localized at the INK4/ARF regulatory domain (RD) identified as a DNA replication origin. (plos.org)
In the absence of Bmi1, M33, or Phc2, primary embryonic fibroblasts (MEFs) are unable to progress into S phase, undergo premature senescence after only a few passages in culture and show an increased accumulation of the tumor suppressors p16 INK4a , p19 ARF and p15 INK4b [4] , [10] . (plos.org)
Here we report that TSC1 and TSC2 regulate Smoothened (SMO)-dependent HH signaling in mouse embryonic fibroblasts (MEFs). (springer.com)
The elongated cilium phenotype of Tsc1 −/− MEFs is likely due to increased mTORC1-dependent autophagic flux observed in these cells, as both the autophagic flux and the cilia length phenotype was restored by rapamycin. (springer.com)
Mouse embyonic fibroblasts (MEFs) were cultured in DMEM + 10% inactivated fetal bovine serum (FBS), penicillin (100U/mL), and streptomycin (100μg/mL) at 37°C, 5% CO2. (nih.gov)

Established by George Todaro and Howard Green in 1962 from disaggregated Swiss mouse ( Mus musculus ) embryo tissue, the 3T3 cell line is a standard fibroblast cell line used in a wide spectrum of research and industrial biomedical applications. (fsu.edu)
Tubulin, Actin, and DNA Distribution in 3T3 Cells - A culture of 3T3 cells was immunofluorescently labeled with primary anti-tubulin mouse monoclonal antibodies followed by goat anti-mouse Fab fragments conjugated to the cyanine dye, Cy3. (fsu.edu)
Visualizing Structural Features of the Golgi Complex and Nucleus in Swiss Mouse Embryo Cells - In order to examine structural features of the Golgi complex and nucleus at relatively high magnification, a log-phase culture of 3T3 cells was fixed, permeabilized, blocked with normal goat serum, and then treated with rabbit anti-giantin (Golgi protein) primary antibodies followed by goat anti-rabbit secondary antibodies (IgG) conjugated to Alexa Fluor 568. (fsu.edu)
Mouse Embryo Fibroblast Cells with Texas Red-X, Alexa Fluor 488, and DAPI - In this section, a Swiss mouse embryo fibroblast cell is presented that was resident in a culture labeled with the fluorophore Texas Red-X conjugated to wheat germ agglutinin, a fluorescent lectin that selectively binds to sialic acid residues. (fsu.edu)
88 TAg Stable Y-LIZ DNA Polymerase-β Knockout Mouse Embryonic Fibroblast Cell Line carries the lambda-LIZ transgene (Y-LIZ), a shuttle vector which enables for mutation analysis. (topsan.org)
T316992 TAg Stable Y-LIZ Wildtype Mouse Embryonic Fibroblast Cell Line carry the lambda-LIZ transgene (Y-LIZ), a shuttle. (topsan.org)
T6080The Stable Bcl-x Knockout Mouse Embryonic Fibroblast Cell Line was generated from embryos of a pregnant mouse heterozygous for. (topsan.org)
T6081The Stable Bcl-xL Mouse Embryonic Fibroblast Cell Line was derived from primary embryonic cells from an 11-d-pregnant Bcl-x. (topsan.org)
T6304To generate the Rieske ISP Stable Knockout Mouse Fibroblast Cell Line, primary lung fibroblast culture was established from a. (topsan.org)
T6303To generate the COX10 Stable Knockout Mouse Fibroblast Cell Line, a primary skin fibroblast culture was established from a knockin mouse. (topsan.org)
An adherent culture of Swiss mouse embryo cells was immunofluorescently labeled with primary anti-vinculin mouse monoclonal antibodies followed by goat anti-mouse Fab heavy and light chain fragments conjugated to Cy3 (red emission). (olympus-lifescience.com)
Repair of mitomycin-C-induced chromosomal aberrations was impaired in RAD51B/Rad51b-c.92delT human and mouse somatic cells in vitro and in explanted mouse bone marrow cells. (nature.com)
Accordingly, Rad51b-c.92delT variant reduced replication fork progression of patient-derived lymphoblastoid cell lines and pluripotent reprogramming efficiency of primary mouse embryonic fibroblasts. (nature.com)
Evidence supporting the direct control of the cell cycle by Pc-G proteins in vertebrates came from studies on mouse Bmi1 mutants. (plos.org)
Conditional Mutation of SMC5 in Mouse Embryonic Fibroblasts. (nih.gov)
Conditional mutation of Smc5 in mouse embryonic stem cells perturbs condensin localization and mitotic progression. (nih.gov)
Using a mouse model, we show that nuclear factor κB activation in Schwann cells is not required for myelination in vivo . (jneurosci.org)
Detailed methods for using ES cells are described in the manual Manipulating the Mouse Embryo: A Laboratory Manual, Second Edition . (cshlpress.com)
This was first shown in mouse embryonic fibroblasts (Loonstra A, et al. (jax.org)
SV40 immortalized mouse embryonic fibroblasts derived from Bax-Bak double-knockout mice were significantly more resistant to GL-induced cell killing compared with wild-type cells. (aspetjournals.org)
His current research projects utilize mouse embryonic stem cells as a model system to investigate the molecular mechanisms that control stem cell differentiation and the development of innate immunity. (usm.edu)
The Molecular Basis for the Lack of Inflammatory Responses in Mouse Embryonic Stem Cells and Their Differentiated Cells. (usm.edu)
Development of Antiviral Innate Immunity During In Vitro Differentiation of Mouse Embryonic Stem Cells. (usm.edu)
Mouse embryonic stem cells are deficient in type I interferon expression in response to viral infections and double-stranded RNA. (usm.edu)
Radiosensitizes both proliferating and quiescent mouse embryonic fibroblast cells to IR and inhibits DSB repair. (fishersci.com)
[9] It is necessary in mouse-feeder cell dependent culture systems, as well as in feeder and serum-free culture systems. (wikidoc.org)
6 Preparation of Mouse Embryonic Fibroblasts for Culture of Human Embryonic Stem Cells ( Meng-Jiao Shi, Maria Borowski, and Kimberly Stencel ). (ecampus.com)
7 Thawing and Seeding of Frozen Mouse Embryonic Fibroblasts ( Meng-Jiao Shi, Maria Borowski, and Kimberly Stencel ). (ecampus.com)
9 Passaging of Human Embryonic Stem Cells on Inactivated Mouse Embryonic Fibroblast Plates ( Meng-Jiao Shi, Kimberly Stencel, and Maria Borowski ). (ecampus.com)
They succeeded in converting one type of mouse pancreatic cell directly into the pancreatic beta cells that produce insulin. (ca.gov)
He reprogrammed mouse fibroblasts directly into primitive heart cells. (ca.gov)
Wild-type (Nrf2(+/+)) mouse embryonic fibroblasts (MEF) produced reactive oxygen species (ROS) at a low level, whereas treatment with Cd significantly increased the ROS production. (cdc.gov)
Induction of the genes required antioxidant response elements (ARE) as Cd drove ARE-dependent reporter expression and Cd-activated Nrf2 bound to endogenous AREs in mouse hepa1c1c7 cells. (cdc.gov)
To track differentiating cell populations, reporter cell lines generated by homologous recombination (knock-in) or via BAC transgenes have been widely used in mouse cells (5-8 ). (lu.se)
In vivo experiments were conducted using a co-implantation xenograft mouse model with live cell imaging and immunohistochemistry. (cdc.gov)

Characterization of embryonic stem cell-differentiated fibroblasts as mesenchymal stem cells with robust expansion capacity and attenuated innate immunity. (usm.edu)
13 Characterization of Human Embryonic Stem Cells by Immunofluorescence Microscopy ( Shirwin M. Pockwinse and Prachi N. Ghule ). (ecampus.com)

4F2 monoclonal antibody recognizes a surface antigen on spread human fibroblasts of embryonic but not of adult origin. (rupress.org)
Moreover, 4F2 antigen was expressed on sarcoma-derived or SV40-transformed adult fibroblastic cells. (rupress.org)
and Neuro-2a cells using TREM2 (E7P8J) Rabbit mAb (Carboxy-terminal Antigen) (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). (cellsignal.com)
The stage-specific embryonic antigen 4 (SSEA4) is com- isolate the NSCs from neonatal mice and rats (Campos monly used as a cell surface marker to identify the pluri- et al. (lu.se)
The stage-specific embryonic antigen 4 (SSEA4) is brain. (lu.se)
Serologic studies for viral antigen detection: Specific antibodies against enteroviral groups and serotypes are commercially available, but their application has thus far been limited to fluorescent staining and detection of viral antigens in cell cultures, rather than clinical specimens. (medscape.com)

Moreover, we show that the Polycomb protein BMI1 interacts with CDC6, an essential regulator of DNA replication in eukaryotic cells. (plos.org)
SC precursors arise during gestation (embryonic day 12-13) and can be identified by their expression of myelin protein zero (MPZ) ( Jessen and Mirsky, 2005 ). (jneurosci.org)
Using a wide range of biochemical and lipidomic analyses, we demonstrated that ABTL0812 increases cellular long-chain dihydroceramides by impairing DEGS1 (delta 4-desaturase, sphingolipid 1) activity, which resulted in sustained ER stress and activated unfolded protein response (UPR) via ATF4-DDIT3-TRIB3 that ultimately promotes cytotoxic autophagy in cancer cells. (nih.gov)
In this study, we found that RING finger protein 4 (RNF4), a RING finger E3 ubiquitin ligase, is required for the RIPK1 autophosphorylation and subsequent cell death. (mdpi.com)
This gene provides instructions for making a protein called fibroblast growth factor receptor 2. (medlineplus.gov)
Among its multiple functions, this protein signals immature cells to become bone cells during embryonic development. (medlineplus.gov)
In the presence of nutrients, mTORC1 is activated and promotes cell growth, including protein synthesis and energy storage. (springer.com)
Conversely, during starvation mTORC1 is inhibited and autophagy is induced by AMP-activated protein kinase (AMPK), leading to generation of intracellular nutrients and energy during degradation of non-functional or non-essential organelles or protein aggregates [ 4 , 6 ], in turn contributing to cell survival. (springer.com)
The induction of proapoptotic Bcl-2 family proteins Bax and Bak and a decrease of antiapoptotic Bcl-2 protein Bcl-2 were observed in GL-treated cells. (aspetjournals.org)
Western blot analysis and in situ hybridization confirmed the presence of AND-34 RNA and protein in lens epithelial cells, particularly at the lens equator. (molvis.org)
Each reagent was used to transfect HeLa cells in a 96-well format at the indicated doses with an emerald green fluorescent protein (GFP)-expressing vector. (thermofisher.com)
The biological effects of FGF1 are mediated through the activation of the four transmembrane phosphotyrosine kinase fibroblast growth factor receptors in the presence of heparin sulfate proteoglycans and, therefore, require the release of the protein into the extracellular space. (rcsb.org)
They develop in a juxtanuclear position, adjacent to the centrosome, the microtubule organizing centre of the cell, and share some protein components. (biorxiv.org)
Despite the long-standing observation that aggresomes/Lewy bodies and the centrosome sit side-by-side in the cell, no studies have been done to see whether these protein accumulations impede the organelle function. (biorxiv.org)
FGF2 , also known as basic fibroblast growth factor (bFGF) and FGF-β, is a growth factor and signaling protein encoded by the FGF2 gene . (wikidoc.org)
Fibroblast growth factor protein was first purified in 1975, but soon afterwards others using different conditions isolated basic FGF, Heparin-binding growth factor-2, and Endothelial cell growth factor-2. (wikidoc.org)
Basic fibroblast growth factor has been shown to interact with casein kinase 2, alpha 1 , [13] RPL6 [14] and ribosomal protein S19 . (wikidoc.org)
Immunoprecipitation of mTOR protein from MCF-7 cell extracts. (cellsignal.com)
The FGFR2 protein is involved in embryonic development, cell division and growth, and cell maturation. (brighthub.com)
The induction of ATF-3 expression was mediated by mitogen-activated protein kinase (MAPK) signaling pathways in GS-002-treated cells. (hindawi.com)

It has been reported that RNF4 negatively regulates TNF-α-induced activation of the nuclear factor-κB (NF-κB) through downregulation of transforming growth factor β-activated kinase 1 (TAK1) activity, indicating the possibility that RNF4-mediated TAK1 suppression results in enhanced sensitivity to cell death. (mdpi.com)
Previous studies reported that ATF-3 was induced by treating cells with antitumorigenic compounds [ 14 - 18 ] and a phosphoinositide 3-kinase inhibitor [ 19 ]. (hindawi.com)

The isolated 3T3 cell presented in the digital image above was resident in an adherent culture stained for F-actin with Alexa Fluor 568 conjugated to phalloidin, and for DNA with 4',6-diamidino-2-phenylindole ( DAPI ). (fsu.edu)
In this report we show that 4F2 mAb specifically binds to the surface of adherent human embryonic fibroblasts but fails to bind to normal adult fibroblasts. (rupress.org)

ABTL0812 is a first-in-class small molecule with anti-cancer activity, which is currently in clinical evaluation in a phase 2 trial in patients with advanced endometrial and squamous non-small cell lung carcinoma ( NCT03366480 ). (nih.gov)
Primary monkey kidney cells or embryonic lung fibroblasts are the standard cells used for isolation and identification of coxsackievirus B. The addition of Buffalo green monkey kidney cells may increase the sensitivity of this method. (medscape.com)
Cancer-associated fibroblasts (CAFs) play a vital role in cancer chemoresistance, but detailed understanding of the process particularly in chemoresistant lung cancer is lacking. (cdc.gov)
In this study, we investigated programmed death-ligand 1 (PDL-1) as a potential biomarker for CAF-induced chemoresistance and evaluated its role and the underlying mechanisms of chemoresistance in non-small cell lung cancer (NSCLC). (cdc.gov)
Silencing PDL-1 expression suppressed CAFs' ability to promote stem cell-like properties and invasiveness of lung cancer cells, favoring chemoresistance. (cdc.gov)
Mechanistically, an upregulation of PDL-1 in chemotherapy-treated CAFs led to an increase in hepatocyte growth factor (HGF) secretion, which stimulates cancer progression, cell invasion, and stemness of lung cancer cells, while inhibiting apoptosis. (cdc.gov)

Finally, addition of 4F2 mAb inhibited the growth of cultured HT-1080 fibrosarcoma cell line, but had no inhibitory effect on various embryonic and adult normal or transformed fibroblasts. (rupress.org)
Fibroblast growth factors, in concert with other growth factors, are key regulators of lens fiber cell differentiation. (nih.gov)
Recent studies implicate other factors [such as fibroblast growth factor (FGFs), hepatocyte growth factor, integrins], present in the lens and ocular environment, in the pathogenesis of ASC and PCO. (nih.gov)
The mTORC1 signaling cascade receives inputs from various upstream cues related to the metabolic and nutritional status of the cell and translates these into cell growth [ 4 , 6 ]. (springer.com)
Furthermore, TSC1 was demonstrated to be required for the proper activation of the transforming growth factor-β (TGF-β)-SMAD2/3 pathway in HeLa cells [ 11 ]. (springer.com)
Expression of AND-34 regulates epithelial cell growth pattern, motility, and growth factor dependence. (molvis.org)
[3] [5] FGF2 binds to and exerts effects via specific fibroblast growth factor receptor (FGFR) proteins which themselves constitute a family of closely related molecules. (wikidoc.org)
Like other FGF family members, basic fibroblast growth factor possess broad mitogenic and cell survival activities, and is involved in a variety of biological processes, including embryonic development , cell growth , morphogenesis , tissue repair , tumor growth and invasion. (wikidoc.org)
the growth factor is necessary for the cells to remain in an undifferentiated state, although the mechanisms by which it does this are poorly defined. (wikidoc.org)
More specifically, it is caused by mutations in the fibroblast growth factor receptor 2 gene ( FGFR2 ), located on chromosome 10. (brighthub.com)
On the other hand, ATF-3 is rapidly induced in cells treated with growth stimulators such as serum and growth factors [ 20 ]. (hindawi.com)
The cancer stem cell theory holds that tumors also contain stem-like cells that drive tumor growth and metastasis formation. (medscape.com)
Kim and Morshead, 2003), and the fibroblast that SSEA4 associated with CD133 can be used for growth factor (FGF) receptor 4 (Cai et al. (lu.se)
Viral growth occurs in 1-4 days, causing a cytopathic effect on the host cells, resulting in rounded, refractile cells that eventually lyse. (medscape.com)

Increasing attention is now being directed at the network of signaling pathways that effect the EMT in lens epithelial cells, with the aim of identifying potential therapeutic targets to inhibit cataract, particularly PCO, which remains a significant clinical problem in ophthalmology. (nih.gov)
While Akt Ser 473 phosphorylation was readily detectable in AND-34 +/+ lens epithelial cells, it was markedly reduced in the AND-34 −/− lens epithelium. (molvis.org)
These results demonstrate the loss of AND-34 dysregulates focal adhesion complex signaling in lens epithelial cells and suggest that AND-34-mediated signaling is required for maintenance of the structural integrity of the adult ocular lens. (molvis.org)

Previously, we showed that ABTL0812 induces TRIB3 pseudokinase expression, resulting in the inhibition of the AKT-MTORC1 axis and macroautophagy/autophagy-mediated cancer cell death. (nih.gov)
Herein, we show that mTOR complex 1 (mTORC1) and mTORC2 have distinct roles in the generation of CD8 + T cell effector and memory populations. (jci.org)
however, due to constitutive mTORC1 activation, these cells retained a terminally differentiated effector phenotype and were incapable of transitioning into a memory state. (jci.org)

Embryonic stem (ES) cells are important vehicles for transfer of genes into mice. (cshlpress.com)
The therapeutic effect of umbilical cord-derived mesenchymal stem cells(hUC-MSCs) in combination with pirfenidone(PFD) on pulmonary fibrosis in mice and its possible mechanism were investigated. (researchsquare.com)
Pitx2(-/-) mice present with severe cardiac malformations and embryonic lethality, demonstrating a role for PITX2 in heart development. (lu.se)
Immunohisto- have been used for positive selection of NSCs from em- chemistry on human embryonic central nervous system bryonic mice (Nagato et al. (lu.se)

The predominant expression pattern of either FL or ED isoforms in single embryonic DRG neurons establishes the existence of two subpopulations exhibiting differential responsiveness to trkB ligands, indicating that regulated splicing of the extracellular domain of trkB may serve as a mechanism to restrict neuronal responsiveness to the neurotrophins. (jneurosci.org)
Since dopaminergic neurons are polarised, ensuring correct localisation of organelles and the effective intracellular transport of neurotransmitter vesicles, loss of centrosome activity could contribute to loss of dopaminergic function and neuronal cell death in Parkinson's disease. (biorxiv.org)
In addition, we provide evidence that many cell types, including dopaminergic neurons, cannot form cilia when aggresomes are present, which would affect their ability to receive extracellular signals. (biorxiv.org)
However, current protocols for deriving neurons from human pluripotent stem cells give rise to heterogeneous cell populations both in regard to the temporal aspects and the cellular composition. (lu.se)
We provide detailed protocols for the generation of lentiviral vectors, the transduction of hES-cells, and their differentia- tion into neurons. (lu.se)
The idea to use transplants of dopa- ment of protocols that allow generation of fully functional mine-producing cells to substitute for the lost midbrain and safe midbrain dopamine neurons from stem cells. (lu.se)
VM), showed that the recovery of motor functions induced implanted either (1) as a solid piece in the lateral ven- by the grafted fetal dopamine neurons was well cor- tricle6 or a cortical cavity8 adjacent to the denervated related with the extent of graft-derived reinnervation caudate-putamen, or (2) as a crude cell suspension of the host caudate-putamen. (lu.se)

X. laevis LG15 cloned adult injected sc with 10,000 15/0 tumor cells. (rochester.edu)
Shinya Yamanaka was the first to reprogram adult cells to an embryonic state called induced pluripotent stem (iPS) cells. (ca.gov)
One way we did this was by converting adult fibroblasts, or skin cells, into interneurons. (lu.se)
Cells from adult organisms that have been reprogrammed into a pluripotential state similar to that of EMBRYONIC STEM CELLS. (bvsalud.org)

2015). Principles of Stem Cell Biology and Cancer: Future Applications and Therapeutics. (wikipedia.org)
X. laevis is the only amphibian where true lymphoid tumors have been discovered and cell lines have been obtained opening new avenues for tumor biology and tumor immunity. (rochester.edu)
Attenuated Innate Immunity in Embryonic Stem Cells and Its Implications in Developmental Biology and Regenerative Medicine. (usm.edu)
Human Stem Cell Technology & Biology: A Research Guide and Laboratory Manual integrates readily accessible text, electronic and video components with the aim of effectively communicating the critical information needed to understand and culture human embryonic stem cells. (ecampus.com)
1 Introduction to Pluripotent Stem Cells: Biology and Applications ( Maria Borowski and Gary S. Stein ). (ecampus.com)
At the core of the network reside Oct4, Sox2 and Nanog, into embryonic stem cells [1,2,3,4,5], have made major inroads which form a self-organized core of the TFs maintaining into stem cell biology. (lu.se)
Combining cell biology and electrophysiology, his work has the potential to create personalized disease models for future research. (lu.se)

Activation of mTOR-dependent pathways regulates the specification and differentiation of CD4 + T effector cell subsets. (jci.org)

HEF, human embryonic fibroblasts. (cdc.gov)
The 4F2 monoclonal antibody (mAb) has been shown to recognize a 120-kilodalton glycoprotein expressed on the cell surface of human peripheral blood monocytes, activated (but not resting) T or B cells, and T and B lymphoblastoid cell lines. (rupress.org)
We report an anticancer effect and mechanism of GL against human prostate cancer cells. (aspetjournals.org)
Treatment with GL significantly inhibited the viability of human prostate cancer cell line LNCaP (androgen-dependent) and its androgen-independent variant (C81) with an IC 50 of ∼1 μM (24-h treatment), at pharmacologically relevant concentrations standardized to its major active constituent z-guggulsterone. (aspetjournals.org)
Chinese hamster ovary (CHO) is one of the most widely used non-human mammalian cell lines. (thermofisher.com)
8 Thawing, Seeding, and Changing the Medium of Human Embryonic Stem Cells ( Meng-Jiao Shi, Kimberly Stencel, and Maria Borowski ). (ecampus.com)
10 Harvesting Human Embryonic Stem Cells for Cryopreservation ( Meng-Jiao Shi, Kimberly Stencel, and Maria Borowski ). (ecampus.com)
11 Human Embryonic Stem Cell Culture on BD Matrigel™with mTeSR®1 Medium ( Meng-Jiao Shi, Kimberly Stencel, and Maria Borowski ). (ecampus.com)
However, Srivastava points out that this cellular career switch has yet to succeed in human cells. (ca.gov)
In this study, we examined the antitumor effects of GS-002 in human hepatoma Hep3B and HepG2 cells in vitro . (hindawi.com)
Many existing protocols for neuronal differentiation of human pluripotent cells result in heterogeneous cell populations and unsynchronized differentiation, necessitating the development of methods for labeling specific cell populations. (lu.se)
This strategy provides a useful tool for visualization and identification of neural progeny derived from human pluripotent stem cells. (lu.se)
We provide detailed protocols for lentiviral transduction, neural differentiation, and subsequent analysis of human embryonic stem cells. (lu.se)
Given this, human pluripotent stem cells, such as human embryonic stem cells (hESCs) and induced pluripo- tent stem cells (hiPSCs), have become a unique cell source for study- ing early human brain development. (lu.se)
Although possible, these strategies are often complicated to transfer to human cells due to technical issues (9 ), and only a few successful cases have been described ( 10, 11 ). (lu.se)
Here we describe how this system can be used for human embry- onic stem cells. (lu.se)
2005). Notch1 and syndecan-1 potent human embryonic stem (ES) cells. (lu.se)
CD133+), but are rarely codetected with the neural stem dents, very few human-specific NSC markers have been cell (NSC) marker CD15. (lu.se)
Human CMV grows only in human cells and replicates best in human fibroblasts. (medscape.com)

Overexposure of cilia in E10.5 Arl13b +/+ , Arl13b V358A/V358A , and Arl13b hnn/hnn embryonic neural tube, stained for IFT88 and ARL13B (NeuroMab). (elifesciences.org)

which means one copy of the altered gene in each cell is sufficient to cause the disorder. (medlineplus.gov)
MATERIALS AND METHODS: A systemic search of gene expression profiles of multiple tissues in NSCLC was carried out to determine the expression intensities of traditional fibroblast biomarkers and CAF-secreted protumorigenic cytokines. (cdc.gov)

The signaling pathways involved in initiating the EMT seem to primarily involve the Smad-dependent pathway, whereby TGFbeta binding to specific high affinity cell surface receptors activates the receptor-Smad/Smad4 complex. (nih.gov)

Our results suggest that in young cells Polycomb proteins are recruited to the INK4/ARF locus through CDC6 and the resulting silent locus is replicated during late S-phase. (plos.org)
Cancer stem cell-associated proteins revealed by proteomics. (medscape.com)

Variants of the initial cell line have been tested and found negative for ectromelia virus (mousepox), but most are susceptible to polyoma and simian virus 40 ( SV40 ). (fsu.edu)

The ability to reprogram fibroblasts into cardiomyocytes has many therapeutic implications. (ca.gov)

However, due to examination of 3T3 cells and subsequent research it has become widely accepted that for immortalization of cells to take place, telomere shortening, which can instigate chromosomal rearrangements, must be overcome, a process that is not necessarily related to a cell s ability to undergo oncogenic transformation. (fsu.edu)

FGFs are essential for early embryonic development, organ formation, and angiogenesis. (rcsb.org)

When combined with normal pre-implantation embryos and transferred to foster mothers, ES cells contribute to the development of many different tissues, including the germline. (cshlpress.com)
For example, it promotes T cell over B cell development from a common precursor (2). (rndsystems.com)

J Cell Sci, 2016 Apr 15. (nih.gov)

Schwann cell isolation. (jneurosci.org)
fication and isolation via fluorescence-activated cell sort- ing (FACS). (lu.se)
Coxsackie B virus infection can be diagnosed by isolation of the virus in cell culture, detection of virus RNA via polymerase chain reaction (PCR) or serologic evaluation of viral antibodies. (medscape.com)

On the other hand, Nrf2 knockout (Nrf2(-/-)) MEF cells exhibited an elevated level of ROS under a basal condition, and Cd dramatically increased the ROS production at concentrations as low as 2 mu M, resulting in increased sensitivity to Cd-induced cell death. (cdc.gov)

Scientists working in basic, translational, and clinical cancer metabolism research are invited to join the Academy in New York on April 17th to discuss the intersection between cell signaling and metabolism. (nyas.org)
Cancer Cell Metabolism: Unique Features Inform New Therapeutic Opportunities. (nyas.org)

The volume will be a valuable reference resource for both experienced investigators pursuing stem cell and induced pluripotent stem cell research as well as those new to this field. (ecampus.com)
These strat- egies provide a robust way to visualize and isolate specific cell pop- ulations of differentiated pluripotent stem cells. (lu.se)
Using this type of direct conversion approach rather than making them from induced pluripotent stem cells, where they revert into a naïve state, helps to retain the epigenetic age of the patient in the generated interneurons. (lu.se)

Avoiding the embryonic state may avoid the tumor-causing potential of embryonic cells and may have other advantages, according to the Gladstone release. (ca.gov)
The CSC is defined as a cancer cell with self-renewing capacity that can regenerate the original tumor in all its differentiated heterogeneity. (medscape.com)
[ 2 ] In addition, some tumor types do not appear to adhere to the CSC concept at all [ 3 , 4 ] or may have multiple independent tumor-initiating cell populations. (medscape.com)

Several main apoptotic indicators were found in GS-002-treated cells, such as the cleaved forms of caspase-3, caspase-9, and poly(ADP-ribose) polymerase (PARP). (hindawi.com)

2002). In humans, SSEA4 is expressed by building the nervous system but also for their prospec- nonneural cells such as the erythrocytes (Kannagi et al. (lu.se)

Fluorescent staining and neutralization assays: Using specific antibodies, these tests are used to further confirm and delineate the type of enterovirus isolated from cell culture. (medscape.com)
Role of PDL-1 in NSCLC chemoresistance was assessed using CRISPR/Cas9 knockdown and various functional assays including MTT, cell invasion, sphere formation, and cell apoptosis. (cdc.gov)

The dogma was once that mature cell types like skin or nerves needed to be reprogrammed to an embryonic-like state before they could mature into a different cell type. (ca.gov)

1983) and the multipotent progenitor cells from fetal disease (Bjorklund and Lindvall, 2000). (lu.se)

Fibroblasts are derived from E 12.5 PKD1 null embryos. (pkd-rrc.org)

When expressed in fibroblasts, the ED isoform exhibited restricted neurotrophin specificity compared with that of the FL receptor. (jneurosci.org)
Pitx2a and Pitx2c are endogenously expressed in C3H10T1/ 2 cells, and these cells express factors that differentially regulate PITX2 isoform activities. (lu.se)

MEF cells can be cultured in vitro in DMEM medium with 10% FBS. (wikipedia.org)

Finally digest the remains by enzymes to obtain single isolated cells and culture the cells in a tissue culture dishes. (wikipedia.org)
3 Basics of Cell Culture ( Alicia Allaire, Mai X. Luong, and Kelly P. Smith ). (ecampus.com)
Reverse transcriptase PCR (RT-PCR) is now an established technique for the detection of viral nucleic acids, especially from specimens with a low viral load that may be associated with false-negative cell culture results. (medscape.com)

When a microRNA is present in the cell, it binds to the target sites and downregulates GFP expression, while in cells that do not express the microRNA GFP, it is expressed. (lu.se)

Hedgehog signaling was restored in Tsc1 −/− cells after exogenous expression of Gli2 , whereas rapamycin restored HH signaling in Tsc 2 −/− cells. (springer.com)

Although the signaling mechanisms governing myelination are not fully understood, NF-κB activation in Schwann cells has been implicated as a key regulator in vitro . (jneurosci.org)
Mechanisms regulating cardiomyocyte cell cycle arrest are of great interest partly because reversing this process could provide a way to stimulate cardiac regeneration after injury [ 17 ]. (springer.com)

It delivers exceptional transfection efficiency with improved cell viability for the widest range of hard-to-transfect and common cell types including HEK 293, HeLa, LNCaP, HepG2, and A549 cell lines (Figure 1) . (thermofisher.com)
Each reagent was used to transfect HEK 293, HeLa, LNCaP, HepG2, and A549 cell lines in a 96-well format, and GFP expression was analyzed 48 hours post-transfection. (thermofisher.com)
Simple Western™ analysis of lysates (0.1 mg/mL) from Hela cells using mTOR (7C10) Rabbit mAb #2983. (cellsignal.com)
Simple Western™ analysis of lysates (1 mg/mL) from HeLa cells using AMPKα (D5A2) Rabbit mAb #5831. (cellsignal.com)
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence ® Control siRNA (Fluorescein Conjugate) #6201 (-) or SignalSilence ® mTOR siRNA II (+), using mTOR (7C10) Rabbit mAb #2983 and α-Tubulin (11H10) Rabbit mAb #2125. (cellsignal.com)

Anatomy48

Organisms21

Diseases10

Chemicals and Drugs79

Analytical, Diagnostic and Therapeutic Techniques and Equipment29

Phenomena and Processes72

Disciplines and Occupations1

Information Science3

Health Care1

Expressed in mouse embryonic fibroblasts2

Swiss Mouse Fibroblast Cells1

Proliferation10

MEFs12

Mouse35

Characterization2

Antigen6

Protein20

Kinase2

Adherent2

Lung6

Growth14

Epithelial Cells3

MTORC13

Mice4

Neurons7

Adult4

Biology7

Regulates1

Human19

Neural1

Gene2

Receptors1

Proteins2

SV401

Cardiomyocytes1

Immortalization1

FGFs1

Development2

20161

Isolation3

Knockout1

Metabolism2

Pluripotent3

Tumor3

Polymerase1

Humans1

Assays2

Type1

Fetal1

Embryos1

Isoform2

DMEM1

Culture3

Binds1

Rapamycin1

Mechanisms2

HeLa5