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  • stem cell lines
  • Similarly, a "parthenote" (derived entirely from one parent) that does not have the potential to develop into a person might be a source of cell lines with potential comparable to that of embryonic stem cell lines. (yale.edu)
  • The disease specific stem cell lines could then be studied in order to better understand the condition. (wikipedia.org)
  • Another application of SCNT stem cell research is using the patient specific stem cell lines to generate tissues or even organs for transplant into the specific patient. (wikipedia.org)
  • While being charged with fraud and embezzlement, he has kept a relatively low profile at the Sooam Bioengineering Research Institute in Yongin, Gyeonggi Province, where he currently leads research efforts on creating cloned pig embryos and using them to make embryonic stem-cell lines. (wikipedia.org)
  • Dolly
  • Using the cloning technique which produced Dolly the sheep in 1996, researchers were able to turn skin cells from a 35-year-old man and a 75-year-old man into stem cells , raising the prospect that body parts could be regenerated in old age. (stem-cells-news.com)
  • In fact, it was the cloning of Dolly that first launched the world into a grand ethical debate over cloning. (scribd.com)
  • One of the most significant times this Commission was put to use was after the cloning of Dolly was announced to the world. (scribd.com)
  • Dolly was cloned by Keith Campbell, Ian Wilmut and colleagues at the Roslin Institute, part of the University of Edinburgh, Scotland, and the biotechnology company PPL Therapeutics, based near Edinburgh. (wikipedia.org)
  • The cell used as the donor for the cloning of Dolly was taken from a mammary gland, and the production of a healthy clone therefore proved that a cell taken from a specific part of the body could recreate a whole individual. (wikipedia.org)
  • Even though Dolly was not the first animal cloned, she received media attention because she was the first cloned from an adult cell. (wikipedia.org)
  • Roslin scientists stated that they did not think there was a connection with Dolly being a clone, and that other sheep in the same flock had died of the same disease. (wikipedia.org)
  • In 2016 scientists reported no defects in thirteen cloned sheep, including four from the same cell line as Dolly. (wikipedia.org)
  • After cloning was successfully demonstrated through the production of Dolly, many other large mammals were cloned, including pigs, deer, horses and bulls. (wikipedia.org)
  • SCNT
  • SCNT involves removing the nucleus of a donor cell and transferring it to an egg cell that has had its nucleus removed. (medicalnewstoday.com)
  • This is the first time that scientists have been able to use SCNT to create human embryonic stem cells (hESCs). (medicalnewstoday.com)
  • The SCNT-hES-1 cells displayed typical ES cell morphology and cell surface markers and were capable of differentiating into embryoid bodies in vitro and of forming teratomas in vivo containing cell derivatives from all three embryonic germ layers in severe combined immunodeficient mice. (sciencemag.org)
  • After continuous proliferation for more than 70 passages, SCNT-hES-1 cells maintained normal karyotypes and were genetically identical to the somatic nuclear donor cells. (sciencemag.org)
  • Although we cannot completely exclude the possibility that the cells had a parthenogenetic origin, imprinting analyses support a SCNT origin of the derived human ES cells. (sciencemag.org)
  • The first study to review the long-term health outcomes of cloning, the authors found no evidence of late-onset, non-communicable diseases other than some minor examples of osteoarthritis and concluded "We could find no evidence, therefore, of a detrimental long-term effect of cloning by SCNT on the health of aged offspring among our cohort. (wikipedia.org)
  • Leading scientists to seek an alternative method of obtaining stem cells, SCNT is one such method. (wikipedia.org)
  • For example, if a person with Parkinson's disease donated his or her somatic cells, the stem cells resulting from SCNT would have genes that contribute to Parkinson's disease. (wikipedia.org)
  • Only a handful of the labs in the world are currently using SCNT techniques in human stem cell research. (wikipedia.org)
  • successfully
  • A number of studies have successfully demonstrated the viability of theoretical proposals previously offered by President Bush's Council on Bioethics to generate alternative sources of pluripotent cells, at least in the experimental setting. (yale.edu)
  • Also, in Korea in 2004, scientist Dr. Hwang Woo Suk published papers that indicated he had successfully cloned human embryos. (scribd.com)
  • Researchers at Oregon Health and Science University have successfully used cloning to create human embryonic stem cells by taking skin cells and fusing them with donated human eggs. (nytimes.com)
  • Hwang first caught media attention in South Korea when he announced he successfully created a cloned dairy cow, Yeongrong-i in February 1999. (wikipedia.org)
  • pluripotency
  • Such a technology would not only provide an ethically acceptable alternative to research cloning, but it would also offer a method to interrogate the biological basis of "sternness," the constellation of gene expression and protein signaling that underlie self-renewal and pluripotency. (yale.edu)
  • A landmark study published in 2006 and many subsequent reports demonstrate that the reactivation of a handful of particular genes can "reprogram" a differentiated cell from a variety of rodent and human tissues into a cell with several properties of embryonic stem cells, including self renewal and pluripotency. (yale.edu)
  • Mouse embryos that are Oct-4-deficient or have low expression levels of Oct-4 fail to form the inner cell mass, lose pluripotency and differentiate into trophectoderm. (wikipedia.org)
  • The pluripotency of these embryonic-like stem cells remains to be fully established. (wikipedia.org)
  • genes
  • He'd like to see a library of cells created with those carefully chosen genes. (wunc.org)
  • Regenerative medicine generally refers to using cells and genes to artificially create tissues and organs and then transplanting them to regenerate physiological functions in patients. (childresearch.net)
  • By culturing ES cells and manipulating the genes in their nuclei, it is possible to develop them into the necessary tissues or organs. (childresearch.net)
  • The EUCOMM program is funded by the European Union sixth R&D programme to make a library of mutant mouse embryonic stem cell clones (ES cells), to enable the mouse research community to rapidly create mutant mice with specific mutant genes. (wikipedia.org)
  • Of these 13000 mutant genes, 8000 mutations in mouse ES Cells are 'targeted': that is, the mutation which knocks out gene function is inserted precisely into the genome. (wikipedia.org)
  • The 8000 genes selected for mutation in mouse ES cells were drawn from the total set of protein coding mouse genes, based on a rough metric of how easy the genes were to mutate, with the technologies present at the time the project started. (wikipedia.org)
  • Viruses offer an efficient means of delivering genes into cells, which they evolved to do, and are thus of great use as experimental tools. (wikipedia.org)
  • This danger can be avoided by the use of viruses which lack key genes, and which are thus unable to replicate after entering a cell. (wikipedia.org)
  • In order to propagate such viral vectors, a cell line that expresses the missing genes is required. (wikipedia.org)
  • Since HEK 293 cells express a number of adenoviral genes, they can be used to propagate adenoviral vectors in which these genes (typically, E1 and E3) are deleted, such as AdEasy. (wikipedia.org)
  • A potential use of stem cells genetically matched to a patient would be to create cell lines that have genes linked to a patient's particular disease. (wikipedia.org)
  • ethical issues
  • On February 24, 1997, President Bill Clinton gave the NBAC 90 days to advise him on ethical issues concerning the cloning of human beings (Eiseman). (scribd.com)
  • Full text of the congressional hearing discussing the ethical issues raised by stem cell research recently conducted in Seoul, South Korea. (iupui.edu)
  • The induced cells also sidestep the ethical issues of embryonic stem cells, which are often created by destroying embryos. (nytimes.com)
  • experiments
  • At present, experiments have succeeded in generating different tissues and organs by exposing ES cells to activin, a particular protein molecule that is secreted from this area. (childresearch.net)
  • In in-vitro experiments of murine Embryonic Stem Cells, Oct-4 has often been used as a marker of stemness, as differentiated cells show reduced expression of this marker. (wikipedia.org)
  • Examples of such experiments include: Effects of a drug on sodium channels Inducible RNA interference system Isoform-selective protein kinase C agonist Interaction between two proteins Nuclear export signal in a protein A more specific use of HEK 293 cells is in the propagation of adenoviral vectors. (wikipedia.org)
  • He was a professor of theriogenology and biotechnology at Seoul National University (dismissed on March 20, 2006) who became infamous for fabricating a series of experiments, which appeared in high-profile journals, in the field of stem cell research. (wikipedia.org)
  • Shortly after that his human cloning experiments were revealed to be fraudulent. (wikipedia.org)
  • fetal
  • In January 2018, a team of scientists in Shanghai announced the successful cloning of two female crab-eating macaques (named Zhong Zhong and Hua Hua) from fetal nuclei. (wikipedia.org)
  • insulin-secre
  • Specifically, this refers to efforts to culture, for example, skin for the treatment of burns, cornea for cataracts, myocardial cells for myocardial disorders, bone and cartilage for motor disorders, neurocytes for neurological disorders, insulin-secreting cells for diabetes, among others. (childresearch.net)
  • world's
  • On November 2015, a Chinese biotech company Boyalife Group announced that it will partner with Hwang's laboratory, Sooam Biotech, to open the world's largest animal cloning factory in Tianjin as early as 2016. (wikipedia.org)
  • differentiation
  • therefore, it is proposed that after directed cell differentiation, the cells could be transplanted without immune rejection to treat degenerative disorders such as diabetes, osteoarthritis, and Parkinson's disease (among others). (sciencemag.org)
  • too much or too little will cause differentiation of the cells. (wikipedia.org)
  • Gene knockdown of Oct-4 promotes differentiation, thereby demonstrating a role for these factors in human embryonic stem cell self-renewal. (wikipedia.org)
  • The intestinal dysplasia resulted from an increase in progenitor cell population and the upregulation of β-catenin transcription through the inhibition of cellular differentiation. (wikipedia.org)
  • During organogenesis, molecular and cellular interactions between germ layers, combined with the cells' developmental potential, or competence to respond, prompt the further differentiation of organ-specific cell types. (wikipedia.org)
  • AS their differentiation into neural and glial cells as well as hepatocyte precursors was observed. (wikipedia.org)
  • There is some debate whether c-Kit is a suitable marker to distinguish amniotic stem cells from other cell types because cells lacking c-Kit also display differentiation potential. (wikipedia.org)
  • transplantation
  • Parkinsons, Alzheimers, diabetes and other degenerative diseases could be treated with cell transplantation of new and healthy cells. (ung.si)
  • immunodeficient mice
  • In 2007, it was confirmed that the amniotic fluid contains a heterogeneous mixture of multipotent cells after it was demonstrated that they were able to differentiate into cells from all three germ layers but they could not form teratomas following implantation into immunodeficient mice. (wikipedia.org)
  • research
  • Now, a team at the Research Institute for Stem Cell Research at CHA Health Systems in Los Angeles and the University of Seoul said they have achieved the same result with two adult males, 'The Telegraph' reported. (stem-cells-news.com)
  • So if you can't do this with adult cells it is of limited value," said Robert Lanza , co-author of the research published in the journal Cell Stem Cell . (stem-cells-news.com)
  • In an interview with NPR, Sean Morrison, Ph.D., Director of the Children's Research Institute at UT Southwestern, joined other stem cell biology experts in discussing the research's implications. (utsw.edu)
  • ban all federal funding for cloning research as well as asking for a temporary voluntary moratorium on cloning research by private institutions until more could be learned about the issue. (scribd.com)
  • The main argument for why cloning research should be continued is the possible benefits it can have. (scribd.com)
  • Human cloning and embryonic stem cell research after Seoul. (iupui.edu)
  • Values in conflict] contains the document "Values in Conflict: Public Attitudes on Embryonic Stem Cell Research. (iupui.edu)
  • Attempts to use either type of cell for therapy remain at the early stages of research, so it is not clear which will turn out to be better. (nytimes.com)
  • To all: This week two bills regarding human embryonic stem cell research and human cloning are likely to receive committee and floor action. (aproundtable.org)
  • Concerns: For the first time in state law, both bills provide an express intent to encourage research on human embryonic stem cells (even though it is limited to the federal registry). (aproundtable.org)
  • SB 210 is even less restrictive, because it would encourage human cloning research with private funds. (aproundtable.org)
  • But the stem cell research component was introduced at the end of September and came specifically from negotiations with Ohio Right to Life as a trade off to stand down on Issue 1. (aproundtable.org)
  • What are the goals of stem cell research? (slidesfinder.com)
  • One of Culver's first initiatives was to sign legislation easing limits on types of stem cell research in Iowa. (wikipedia.org)
  • Culver proposed spending $12.5 million to establish a stem cell research center at the University of Iowa. (wikipedia.org)
  • The European Conditional Mouse Mutagenesis Program or EUCOMM is an EU-funded program to generate a library of mutant mouse embryonic stem cells for research purposes. (wikipedia.org)
  • Bauer opposes cloning and embryonic stem cell research, but supports adult stem cell research. (wikipedia.org)
  • HEK 293 cells have been widely used in cell biology research for many years, because of their reliable growth and propensity for transfection. (wikipedia.org)
  • She was responsible for drafting both the 2000 and 2009 NIH Guidelines for Research Using Human Embryonic Stem Cells. (wikipedia.org)
  • He's been an outspoken proponent of the Catholic Church's positions in opposition to human cloning and embryonic stem cell research. (wikipedia.org)
  • On May 12, 2006, Hwang was charged with embezzlement and bioethics law violations after it emerged much of his stem cell research had been faked. (wikipedia.org)
  • The Korea Times reported on June 10, 2007, that Seoul National University fired him, and the South Korean government canceled his financial support and barred him from engaging in stem cell research. (wikipedia.org)
  • In February 2011, Hwang visited Libya as part of a $133 million project in the North African country to build a stem cell research center and transfer relevant technology. (wikipedia.org)
  • His alleged success was touted as the fifth instance in the world in cow cloning, with a notable caveat: Hwang failed to provide scientifically verifiable data for the research, giving only media sessions and photo-ops. (wikipedia.org)
  • It has also made approaches in connection to ethical and bioethical questions in the Brazilian social-political context, such as decision about anencephalic fetus, stem cell research and ethics in public health parting from a critical point of view from iluminism. (wikipedia.org)
  • Dolly's
  • Dolly's birth set off a huge outpouring of ethical concern - along with hope that the same techniques, applied to human cells, could be used to treat myriad diseases. (wunc.org)
  • The funding for Dolly's cloning was provided by PPL Therapeutics and the UK's Ministry of Agriculture. (wikipedia.org)
  • Some in the press speculated that a contributing factor to Dolly's death was that she could have been born with a genetic age of six years, the same age as the sheep from which she was cloned. (wikipedia.org)
  • human cell
  • And with a bit of coaxing, these cells could, theoretically, be prodded to turn into any sort of human cell - nerve, heart, liver and pancreas, for example. (wunc.org)
  • Graham and coworkers provided evidence that HEK 293 cells and other human cell lines generated by adenovirus transformation of human embryonic kidney cells have many properties of immature neurons, suggesting that the adenovirus preferentially transformed a neuronal lineage cell in the original kidney culture. (wikipedia.org)
  • mammals
  • Many vertebrates such as fish, birds and amphibians do not suffer presbycusis in old age as they are able to regenerate their cochlear sensory cells, whereas mammals including humans have genetically lost this ability. (wikipedia.org)
  • nuclear
  • Eighteen years ago, scientists in Scotland took the nuclear DNA from the cell of an adult sheep and put it into another sheep's egg cell that had been emptied of its own nucleus. (wunc.org)
  • Writing in the journal Cell Stem Cell , they say they started with nuclear DNA extracted from the skin cells of a middle-age man and injected it into human eggs donated by four women. (wunc.org)
  • Embryonic-like stem cells were originally identified using markers common to embryonic stem cells such as nuclear Oct4, CD34, vimentin, alkaline phosphatase, stem cell factor and c-Kit. (wikipedia.org)
  • The reprogramming process that cells need to go through during cloning is not perfect and embryos produced by nuclear transfer often show abnormal development. (wikipedia.org)
  • diseases
  • So ideally scientists would like to be able to extract DNA from the cells of older people - not just cells from infants - to create therapies for adult diseases. (wunc.org)
  • Such stem cells can regenerate and replace those damaged cells and tissues and alleviate diseases that affect millions of people. (medicalnewstoday.com)
  • neural
  • It was later deterimined that only two of these four factors, Oct4 and Klf4 were sufficient to reprogram mouse adult neural stem cells. (wikipedia.org)
  • In vertebrates, a special population of embryonic cells called the neural crest has been proposed as a "fourth germ layer", and is thought to have been an important novelty in the evolution of head structures. (wikipedia.org)
  • Although those cells which expressed the markers were able to differentiate into muscle, adipogenic, osteogenic, nephrogenic, neural and endothelial cells, this did not necessarily occur from a homogenous population of undifferentiated cells. (wikipedia.org)
  • genetic
  • They repeated the process - this time starting with the genetic material extracted from the skin cells of a much older man. (wunc.org)
  • generate
  • Dr. Mitalipov said the technique was efficient enough that one donation - which can include multiple eggs - would probably be enough to generate a stem cell line, even accounting for failures. (nytimes.com)
  • The Potential of Stem Cells It may become possible to generate healthy heart muscle cells in the laboratory and then transplant those cells into patients with chronic heart disease. (slidesfinder.com)
  • germ cells
  • Orthologs of Oct-4 exist in humans and several other species including: Oct-4 contains the following protein domains: Oct-4 has been implicated in tumorigenesis of adult germ cells. (wikipedia.org)
  • Even within humans and other mortal species, there are cells with the potential for immortality: cancer cells which have lost the ability to die when maintained in a cell culture such as the HeLa cell line, and specific stem cells such as germ cells (producing ova and spermatozoa). (wikipedia.org)
  • vitro
  • Even in the Petri dish, the fertilized egg created through in vitro fertilization continues to divide, with the number of cells doubling quickly. (childresearch.net)
  • As a consequence, HEK 293 cells should not be used as an in vitro model of typical kidney cells. (wikipedia.org)
  • create
  • They injected it into 77 human egg cells, and from all those attempts, managed to create two viable cells that contained DNA from one or the other man. (wunc.org)
  • This ability allows stem cells to create any cell type, which could then be transplanted to replace damaged or destroyed cells. (wikipedia.org)
  • Hwang explained that his team used 242 eggs to create a single cell line. (wikipedia.org)
  • viable
  • The attempt to clone argali (mountain sheep) did not produce viable embryos. (wikipedia.org)
  • The attempt to clone a banteng bull was more successful, as were the attempts to clone mouflon (a form of wild sheep), both resulting in viable offspring. (wikipedia.org)
  • this approach has been championed as an answer to the many issues concerning embryonic stem cells (ESC) and the destruction of viable embryos for medical use, though questions remain on how homologous the two cell types truly are. (wikipedia.org)
  • attempts
  • Though neither of these attempts were successful, both show that attempts on human cloning will still occur as long as there is no worldwide legislation. (scribd.com)
  • fetus
  • In humans, a pregnancy is generally considered to be in the embryonic stage of development between the fifth and the eleventh weeks after fertilization, and is expressed as a fetus from the twelfth week. (wikipedia.org)
  • gene
  • For the National Institutes of Health, Trump and the Republican-led Congress are likely to revisit funding for embryonic stem cell research and to take a closer look at restricting gene editing. (northeastern.edu)
  • This previously unidentified progenitor occurs as a temporally discrete population during ES cell differentiation, and differs from the preceding and succeeding populations in gene expression and differentiation potential, with the unique ability to form surface ectoderm in response to BMP4 signalling. (biologists.org)
  • Research applications such as gene editing, gene expression, and directed differentiation depend on the effective, efficient delivery of DNA, RNA and/or Cas9 protein complexes into stem cells. (thermofisher.com)
  • In addition, Lipofectamine Stem reagent can be used for the transfection of mRNA for gene expression or directed differentiation studies using stem cells. (thermofisher.com)
  • in 9 days (FS) showed that the Ped candidate gene is present in FF and FS but not in 8-cell embryos. (usp.br)
  • The protein encoded by this gene is found in rod cells in the back of the eye and is essential for vision in low-light conditions. (genecards.org)
  • This discovery changed the current thinking about cancer from a model wherein cancer is caused by a foreign substance (a viral gene) to one where a gene that is normally present in the cell can cause cancer. (wikipedia.org)
  • Other studies showed that lack of Daxx gene caused a higher apoptotic rate in embryonic stem cells. (wikipedia.org)
  • Although senescent cells can no longer replicate, they remain metabolically active and commonly adopt an immunogenic phenotype consisting of a pro-inflammatory secretome, the up-regulation of immune ligands, a pro-survival response, promiscuous gene expression (pGE) and stain positive for senescence-associated β-galactosidase activity. (wikipedia.org)
  • 1999
  • Going into the 1999 election with new leadership and executive, the party achieved limited media attention by conducting a demonstration at Queen's Park featuring three "cloned sheep" to represent Progressive Conservative leader Mike Harris, Liberal leader Dalton McGuinty and New Democratic Party leader Howard Hampton. (wikipedia.org)
  • stem cell res
  • All of these attributes make this versatile reagent an ideal addition to your stem cell research tool kit and a readily accessible alternative to electroporation. (thermofisher.com)
  • Proposed by Honduras, the statement was largely supported by Roman Catholic countries and opposed by countries with active embryonic stem cell research programs. (wikipedia.org)
  • The European Union has yet to issue consistent regulations with respect to stem cell research in member states. (wikipedia.org)
  • France prohibits reproductive cloning and embryo creation for research purposes, but enacted laws (with a sunset provision expiring in 2009) to allow scientists to conduct stem cell research on imported a large amount of embryos from in vitro fertilization treatments. (wikipedia.org)
  • Germany has restrictive policies for stem cell research, but a 2008 law authorizes "the use of imported stem cell lines produced before May 1, 2007. (wikipedia.org)
  • According to modern stem cell researchers, Spain is one of the leaders in stem cell research and currently has one of the most progressive legislations worldwide with respect to hESC research. (wikipedia.org)
  • The United Kingdom is one of the leaders in stem cell research, in the opinion of Lord Sainsbury, Science and Innovation Minister for the UK. (wikipedia.org)
  • A new £10 million stem cell research centre has been announced at the University of Cambridge. (wikipedia.org)
  • mice
  • Mice deficient for Mxd3 show increased sensitivity of neuronal and lymphoid cells to gamma-radiation induced apoptosis. (uniprot.org)
  • The experimental elimination of senescent cells from transgenic progeroid mice and non-progeroid, naturally-aged mice led to greater resistance against aging-associated diseases. (wikipedia.org)
  • Gli2-/- and Gli3-/- double homozygous transgenic mice are not viable and do not survive beyond embryonic level. (wikipedia.org)
  • Transgenic mice over-expressing the transcription factor Gli2 under the K5 promoter in cutaneous keratinocytes develop multiple skin tumours on the ears, tail, trunk and dorsal aspect of the paw, resembling those of basal cell carcinoma (BCC). (wikipedia.org)
  • After modifying the technique, Obokata was able to show that white blood cells from newborn mice could be transformed into cells that behaved much like stem cells. (wikipedia.org)
  • This was the first in a series of development that have begun to make rats tractable as genetic research subjects, although they still lag behind mice, which lend themselves better to the embryonic stem cell techniques typically used for genetic manipulation. (wikipedia.org)
  • lineage
  • Using a previously established mouse ES-cell-based system that recapitulates the development of the ectoderm lineage we have identified a transient population that is consistent with definitive ectoderm. (biologists.org)
  • In Xenopus , the germline is specified through the inheritance of germ plasm formed during oogenesis and asymmetrically segregated into the future germ cell lineage. (biologists.org)
  • May play a role in spatial programing within discrete embryonic fields or lineage compartments during organogenesis. (genecards.org)
  • chromosome
  • In human fertilization, a released ovum (a haploid secondary oocyte with replicate chromosome copies) and a haploid sperm cell (male gamete)-combine to form a single 2n diploid cell called the zygote. (wikipedia.org)
  • inducible
  • This means that in these cell types long term tetracycline inducible expression can be achieved. (clontech.com)
  • When expressing the Tet-On 3G transactivator protein from the EF-1 alpha promoter, 83% of the Jurkat Tet-On 3G clones showed strong inducible expression and 33% demonstrated very high inducibility (greater than 2,000-fold). (clontech.com)
  • plasmid
  • Time-lapse phase-contrast (left) and fluorescence (right) movies of human ES cells (H9) grown in Essential 8 medium on vitronectin and transfected with 1 µL Lipofectamine Stem reagent complexed with a GFP-expression construct (500 ng total plasmid DNA). (thermofisher.com)
  • The goal of this study was to illustrate the versatility of Invitrogen Lipofectamine Stem Transfection Reagent, which was developed specifically for stem cells, to co-deliver multiple payloads in addition to large plasmid constructs with high transfection efficiency. (thermofisher.com)
  • (A) Human ES (H9) cells or Human Episomal iPSCs were transfected with Lipofectamine Stem Reagent or FuGENE HD Regent according to the accompanying protocol and either a 5 kb or 11 kb DNA plasmid expressing GFP and examined for GFP expression 24 hours posttransfection. (thermofisher.com)
  • In the bottom panel NCRM-iPS cells transfected using Lipofectamine Stem Reagent with a ~10.5 kb plasmid expressing Cas9 and GFP followed by detection via immunocytochemistry. (thermofisher.com)
  • heterochromatin
  • The nucleus of senescent cells is characterized by senescence-associated heterochromatin foci (SAHF) and DNA segments with chromatin alterations reinforcing senescence (DNA-SCARS). (wikipedia.org)
  • Proteins
  • Gli family zinc finger proteins are mediators of Sonic hedgehog (Shh) signaling and they are implicated as potent oncogenes in the embryonal carcinoma cell. (wikipedia.org)
  • In the field of molecular biology, nuclear receptors are a class of proteins found within cells that are responsible for sensing steroid and thyroid hormones and certain other molecules. (wikipedia.org)
  • cellular
  • TGF-β regulates a variety of different cellular developmental processes including growth, differentiation, proliferation, and cell death. (wikipedia.org)
  • Cellular senescence is the phenomenon by which normal diploid cells cease to divide. (wikipedia.org)
  • As such, cellular senescence represents a change in "cell state" rather than a cell becoming "aged" as the name confusingly suggests. (wikipedia.org)
  • Consistent with this, telomerase-immortalised cells continued to age (according to the epigenetic clock) without having been treated with any senescence inducers or DNA-damaging agents, re-affirming the independence of the process of epigenetic ageing from telomeres, cellular senescence, and the DNA damage response pathway. (wikipedia.org)
  • Mitochondrial DNA (mtDNA or mDNA) is the DNA located in mitochondria, cellular organelles within eukaryotic cells that convert chemical energy from food into a form that cells can use, adenosine triphosphate (ATP). (wikipedia.org)
  • Stem cells are cells found in most, if not all, multi-cellular organisms. (wikipedia.org)
  • eukaryotic cell
  • A zygote (from Greek ζυγωτός zygōtos "joined" or "yoked", from ζυγοῦν zygoun "to join" or "to yoke") is a eukaryotic cell formed by a fertilization event between two gametes. (wikipedia.org)
  • proliferation
  • of TRPC decreased the cell routine S stage and cell migration, implicating an operating function for TRP-mediated Ca2+ entrance in cell proliferation and invasion. (cancercurehere.com)
  • Exogenous PUFA and a TRPC3 antagonist regularly attenuated breasts cancer tumor cell proliferation and migration, recommending a mechanism where PUFA restrains the breasts cancer partially via its inhibition of TRPC stations. (cancercurehere.com)
  • Ca2+ entrance via turned on TRPC was improved when PUFA had been absent, recommending a double-gating system for Danshensu TRPC which may be involved with MCF breasts cancer tumor cell proliferation and invasion. (cancercurehere.com)
  • Lipofectamine Stem reagent can be used to transfect a wide range of stem cell types with superior efficiency while supporting continued proliferation without inducing differentiation. (thermofisher.com)
  • Human
  • (B) Human ES (H9) cells were transfected with Lipofectamine Stem Reagent or FuGENE HD Reagent according to the protocol with an mRNA expressing GFP. (thermofisher.com)
  • The United Nations Declaration on Human Cloning was a nonbinding statement against all forms of human cloning approved by a divided UN General Assembly. (wikipedia.org)
  • The UN Declaration on Human Cloning, as it is named, calls for all member states to adopt a ban on human cloning, which it says is "incompatible with human dignity and the protection of human life. (wikipedia.org)
  • After DNMT1 knockout in human cancer cells, these cells were found to retain their inherited methylation pattern, which suggests maintenance activity by the expressed DNMT3s. (wikipedia.org)
  • Invitrogen
  • MCF-7 cells had Danshensu been passaged onto coverslips in 500 l Opti-MEM (Invitrogen) 1 day before transfection and reached about 40C50% confluence during transfection. (cancercurehere.com)
  • Designed for dramatically improved transfection efficiency, Invitrogen Lipofectamine Stem Transfection Reagent offers a simple, robust and reproducible method for delivering DNA, RNA and RNP such as Cas9/gRNA complexes into a wide range of stem cells, including pluripotent stem cells, neural stem cells and mesenchymal stem cells (Figure 2) . (thermofisher.com)
  • diploid
  • The result of karyogamy is the formation of a diploid cell called zygote or zygospore. (wikipedia.org)
  • After approximately 30 hours from the time of fertilization, fusion of the pronuclei and immediate mitotic division produce two 2n diploid daughter cells called blastomeres. (wikipedia.org)
  • sperm
  • Italy has a 2004 law that forbids all sperm or egg donations and the freezing of embryos, but allows, in effect, using existing stem cell lines that have been imported. (wikipedia.org)
  • In seedless plants, the archegonium is usually flask-shaped, with a long hollow neck through which the sperm cell enters. (wikipedia.org)
  • protein
  • C, left) Lipofectamine Stem Reagent was used to transfect cells with Cas9 mRNA (modified)/ gRNA: Emx-1 crRNA (Exon2)-tracrRNA oligo/ GFP mRNA (modified) or Cas9 protein/gRNA: Emx-1 crRNA (Exon2)-tracrRNA oligo/ GFP mRNA (modified). (thermofisher.com)
  • Alkaline phosphatase in E. coli is located in the periplasmic space and can thus be released using techniques that weaken the cell wall and release the protein. (wikipedia.org)
  • viability
  • This newest addition to the Lipofectamine transfection reagent family helps ensure high-efficiency transfection while maintaining maximum cell viability and growth in an undifferentiated state. (thermofisher.com)
  • Required for photoreceptor cell viability after birth. (genecards.org)
  • divisions
  • In normal circumstances, absent telomerase, if a cell divides recursively, at some point the progeny reach their Hayflick limit, which is believed to be between 50-70 cell divisions. (wikipedia.org)
  • oncogenes
  • Cells can also be induced to senesce via DNA damage in response to elevated reactive oxygen species (ROS), activation of oncogenes and cell-cell fusion, independent of telomere length. (wikipedia.org)
  • mitosis
  • This cell may then enter meiosis or mitosis depending on the life cycle of the species. (wikipedia.org)
  • Telomerase replaces short bits of DNA known as telomeres, which are otherwise shortened when a cell divides via mitosis. (wikipedia.org)
  • membrane
  • In Gram-negative bacteria, such as Escherichia coli (E. coli), alkaline phosphatase is located in the periplasmic space, external to the inner cell membrane and within the peptidoglycan portion of the cell wall. (wikipedia.org)
  • fluorescence
  • Adjustments in the Ca2+ focus had been indicated as F/F, where F was the fluorescence strength when cells had been at rest, and F was the modification in fluorescence during excitement. (cancercurehere.com)
  • subsequently
  • The technique for producing STAP cells was subsequently studied by Obokata at the Brigham and Women's Hospital (BWH), while she was studying as a post doc under Charles Vacanti, and then at the RIKEN Center for Developmental Biology in Japan. (wikipedia.org)
  • functional
  • Within this research, we discovered the functional appearance of TRPC3 in individual MCF-7 breasts cancer tumor cell-mediated Ca2+ entrance. (cancercurehere.com)
  • found
  • This partnership is found mainly in the S-phase of the cell cycle. (wikipedia.org)
  • On August 5, 2014, Yoshiki Sasai-Obokata's supervisor at RIKEN and one of the coauthors on the STAP cell papers-was found dead at a RIKEN facility after an apparent suicide by hanging. (wikipedia.org)
  • Indeed, it was found that differentiation was partially rescued if Dnmt3a-/- HSCs experienced an additional Ctnb1 knockdown - Ctnb1 codes for β-catenin, which participates in self-renewal cell division. (wikipedia.org)
  • It has also been found that DNMT3A-mutated cell lines exhibit transcriptome instability, in that they have much more erroneous RNA splicing as compared to their isogenic[disambiguation needed] wildtype counterparts. (wikipedia.org)
  • reagent
  • Cell routine evaluation After removal of methanol, cells had been treated having a Coulter DNA-Prep reagent package (Beckman-Coulter, France). (cancercurehere.com)