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  • cardiac
  • The scaffolds were successfully reseeded with different cellular sources derived from pluripotent stem cells to achieve beating cardiac tissues characterized by endothelial, cardiac, and smooth muscle markers. (hindawi.com)
  • During differentiation, cardiac-specific genes as well as proteins, receptors, and ion channels are expressed in a developmental continuum, which closely recapitulates the developmental pattern of early cardiogenesis. (ahajournals.org)
  • Exploitation of ES cell-derived cardiomyocytes has facilitated the analysis of early cardiac development and has permitted in vitro "gain-of-function" or "loss-of-function" genetic studies. (ahajournals.org)
  • Recently, human ES cell lines have been established that can be used to investigate cardiac development and the function of human heart cells and to determine the basic strategies of regenerative cell therapy. (ahajournals.org)
  • Using this information, we identified 43 genes not previously associated with cardiac ESC differentiation for which we were able to assign a putative novel biological function. (wur.nl)
  • Diverse types of stem cells represent a potentially attractive source of cardiac cells for the treatment of cardiovascular diseases. (mdpi.com)
  • The effects of inoculating different cell numbers, time of EB adherence to gelatin-coated dishes, and rotation speed for optimal EB formation and cardiac differentiation were investigated. (springer.com)
  • Using 3 × 10 5 cells/ml, 10 rpm rotary speed and plating of EBs onto gelatin-coated surfaces three days after culture, were the best parameters for optimal size and EB quality on consequent cardiac differentiation. (springer.com)
  • These optimized parameters enrich cardiac differentiation in ES cells when using the STLV method. (springer.com)
  • endothelial
  • Endothelial cells typically comprise 15-20% of the differentiated ES cells. (springer.com)
  • 1994) Platelet endothelial cell adhesion molecule-1 (PECAM/CD31): alternatively spliced, functionally distinct isoforms expressed during mammalian cardiovascular development. (springer.com)
  • This process is recapitulated in the adult when endothelial progenitor cells are generated in the bone marrow and can contribute to vascular repair or angiogenesis at sites of vascular injury or ischemia. (harvard.edu)
  • Novel approaches are needed to identify the factors that regulate endothelial differentiation. (harvard.edu)
  • A separate population of VEGF-R2+ stem cells expressing the endothelial-specific marker CD144 (VE-cadherin) was also identified at this same time point. (harvard.edu)
  • Expression profiling confirmed the role of several genes involved in hematopoiesis, and identified several putative genes involved in endothelial differentiation. (harvard.edu)
  • Conclusion: The isolation of CD144+ cells during ES cell differentiation from embryoid bodies provides an excellent model system and method for identifying genes that are expressed during endothelial differentiation and that are distinct from hematopoiesis. (harvard.edu)
  • progenitors
  • Therefore, embedding stem cells within a tissue-specific environment matched to the developmental stage of the progenitors may offer a practical solution for stem-cell-derived applications such as disease modeling, pharmaceutical safety testing, and screening of novel therapeutic targets. (hindawi.com)
  • Bouhon IA, Joannides A, Kato H, Chandran S, Allen ND (2006) Embryonic stem cell-derived neural progenitors display temporal restriction to neural patterning. (springer.com)
  • Lentiviral silencing of FoxO1 in pancreatic progenitors upregulated the levels of pancreatic islet differentiation-related genes and improved glucose-stimulated insulin secretion response in their progeny IPCs, whereas overexpression of FoxO1 showed the opposite effects. (ovid.com)
  • cultures
  • Conventional techniques, such as hanging drop and suspension cultures, do not allow precise control over size of ES cell aggregates. (rsc.org)
  • Using transgenic mice and cell cultures, we found that Notch has complex and extensive regulatory functions in Schwann cells. (epfl.ch)
  • ES cell density does not influence β-catenin expression, but a greater proportion ofβ -catenin is targeted for degradation in high-density cultures. (biologists.org)
  • RT-PCR analysis of differentiated cultures revealed expression of markers of early pancreas differentiation (HNF3beta and pdx-1) in all spontaneously differentiated cell (SDC) cultures but very low expression in undifferentiated cells. (endocrine-abstracts.org)
  • Insulin and glucagon expression was detected in one of the SDC cultures and components of the beta-cell ATP sensitive potassium (K ATP ) channel , Kir6.2 and SUR1, were detected in all SDC cultures. (endocrine-abstracts.org)
  • mesoderm
  • I attempted to enhance the commonly used growth factor-driven mesoderm and DE differentiation protocols using hit molecules acquired from the HTS, and identified MEG as a potent enhancer of growth factor-induced mesoderm and DE differentiations. (illinois.edu)
  • induction
  • Interestingly, basal levels of reactive oxygen species (ROS) were not altered in ESCs, but Nsun3 inactivation attenuated induction of mitochondrial ROS upon stress, which may affect gene expression programs upon differentiation. (springer.com)
  • Although ES cells are clonally established, they display heterogeneous responses upon the induction of differentiation, resulting in a mixture of various types of differentiated cells. (mdpi.com)
  • novel
  • Albelda, S. M., Muller, W. A., Buck, C. A., and Newman, P. J. (1991) Molecular and cellular properties of PECAM-1 (endoCAM/CD31): a novel vascular cell-cell adhesion molecule. (springer.com)
  • molecular
  • We anticipate that these 43 genes can help to improve the understanding of the molecular events underlying ESC differentiation. (wur.nl)
  • They describe their findings June 5 in Molecular Cell . (uchospitals.edu)
  • For the goal of using stem cells in therapy, the most important step is differentiation from iPSCs," said Rui Zhao, Ph.D., an assistant professor of biochemistry and molecular genetics at the University of Alabama at Birmingham . (uab.edu)