• Induces hyperpolarization to the same magnitude as ACh in aortic value endothelial cells. (fishersci.com)
  • The surgical procedure for delivery of embryonic stem cell-derived endothelial cells to the ischemic hindlimb is demonstrated, with non-invasive tracking by bioluminescence imaging. (jove.com)
  • Does not induce differentiation of vascular smooth muscle cells or endothelial cells. (tocris.com)
  • In particular, FUNGENES identified genes controlling the development of pluripotent embryonic stem (ES) cells into heart cells (cardiomyocytes), nerve cells (neurons), smooth muscle cells, vascular endothelial cells, fat cells (adipocytes), liver cells (hepatocytes) and insulin-producing cells of the pancreas. (europa.eu)
  • The manipulation of endothelial cells to secrete protective factors could enhance cardioprotection. (mdpi.com)
  • Furthermore, we have shown that transplantation of immature cardiovascular progenitors may generate cardiomyocytes in addition to supporting cells such as vascular endothelial cells and fibroblasts. (ca.gov)
  • These dental stem cells are considered mesenchymal stem cells (MSCs) and possess different levels of capacities to become specific tissue forming cells. (bvsalud.org)
  • He has been leader of a research group at the Max-Delbrück-Center for Molecular Medicine in Berlin until 2011 focusing his research on signal transduction mechanisms in human and murine embryonic stem cells. (eurostemcell.org)
  • Role of natural-killer group 2 member D ligands and intercellular adhesion molecule 1 in natural killer cell-mediated lysis of murine embryonic stem cells and embryonic stem cell-derived cardiomyocytes. (tum.de)
  • Dr. Jun's research group focuses on nanostructured biomaterials and stem-cell-based tissue regeneration to develop an innovative strategy to treat various diseases. (uab.edu)
  • Prof. Eldad Tzahor of the Institute's Biological Regulation Department thought that part of the answer to the regeneration puzzle might lie in his area of expertise: embryonic development, especially of the heart. (eurekalert.org)
  • Researchers at Cincinnati Childrens Hospital Medical Center and the Howard Hughes Medical Institute (HHMI) claim that cardiac stem cells used in ongoing clinical trials - which express a protein marker called c-kit - do not achieve high enough regeneration rates to justify their use. (pennywarren.co.uk)
  • Regeneration rates were measured during embryonic development, ageing and after myocardial infarction (heart attack). (pennywarren.co.uk)
  • Here, we utilize in vivo biophysical and genetic fate mapping zebrafish studies to reveal that altered hemodynamic forces due to cardiac injury activate a sequential endocardial-myocardial signaling cascade to direct cardiomyocyte reprogramming and heart regeneration. (elifesciences.org)
  • Consequently, Klf2a then activates endocardial Notch signaling which results in the non-cell autonomous initiation of myocardial Erbb2 and BMP signaling to promote cardiomyocyte reprogramming and heart regeneration. (elifesciences.org)
  • Overall, these findings not only reveal how the heart senses and adaptively responds to environmental changes due to cardiac injury, but also provide insight into how flow-mediated mechanisms may regulate cardiomyocyte reprogramming and heart regeneration. (elifesciences.org)
  • This endocardial Notch signaling can in turn non-cell autonomously initiate myocardial Erbb2 and BMP signaling to promote cardiomyocyte reprogramming and heart regeneration, whereas its inhibition through either using tissue-specific genetic-based strategies or altering blood flow impairs this reparative event. (elifesciences.org)
  • Progenitor cell therapy describes the use of multipotent cells of various cell lineages (autologous or allogeneic) for tissue repair and/or regeneration. (southcarolinablues.com)
  • 1 Treatment with progenitor cells (i.e., stem cells) offers potential benefits beyond those of standard medical care, including the potential for repair and/or regeneration of damaged myocardium. (southcarolinablues.com)
  • However, no study has yet confirmed the delivery of a pure population of stem cells capable of robust regeneration of the injured myocardium. (ca.gov)
  • While adult stem cells have yet failed to convincingly regenerate myocardial tissue, human embryonic stem cells (hESCs) have proven to be a potential and unlimited source for cardiomyocyte regeneration. (ca.gov)
  • These results highlight the potential benefit of progenitor cells to generate other cell types that contribute to heart regeneration. (ca.gov)
  • A major challenge to clinical translation of stem cells for heart regeneration is the lack of data on the integration of the transplanted cells into the host heart. (ca.gov)
  • There are limited numbers of publications about ES cells in pulp regeneration, due to the restricted policies regarding ES cell research over the past few years. (bvsalud.org)
  • In contrast, augmentation of endogenous Notch reactivation after myocardial infarction in the adult, either by inducing cardiomyocyte-specific Notch1 transgene expression or by intramyocardial delivery of a Notch1 pseudoligand, increases survival rate, improves cardiac functional performance, and minimizes fibrosis, promoting antiapoptotic and angiogenic mechanisms. (monash.edu)
  • Among the current biomaterial applications, the use of biopolymers as cell scaffolds could aid the development of stem cell-based therapies aimed at replacing tissue loss due to injuries or diseases, such as during myocardial infarction. (jscimedcentral.com)
  • Prof. Mummery has pioneered studies on cardiomyocytes from human embryonic stem cells (hPSC) and was among the first to inject them in mouse heart after myocardial infarction. (wikipedia.org)
  • Mummery was the first to derive human-induced pluripotent stem cells (hiPSC) in Netherlands and is leading in their use for cardiovascular disease modelling and safety pharmacology. (wikipedia.org)
  • Amend N, Thiermann H, Worek F, Wille T ( 2019 ) The arrhythmogenic potential of nerve agents and a cardiac safety profile of antidotes - A proof-of-concept study using human induced pluripotent stem cells derived cardiomyocytes (hiPSC-CM). Toxicol Lett 308:1-6. (multichannelsystems.com)
  • Cells from the blood samples are reprogrammed into clinical-grade human induced pluripotent stem cells, which can be distributed to recipients for regenerative medicine. (wjgnet.com)
  • Modeling genetic cardiac channelopathies using induced pluripotent stem cells - Status quo from an electrophysiological perspective. (uni-koeln.de)
  • Cyclooxygenases Inhibitors Efficiently Induce Cardiomyogenesis in Human Pluripotent Stem Cells. (uni-koeln.de)
  • Pluripotent stem cells (PSCs) harbor several attractive features for regenerative medicine: they are capable of self-renewal and have the capacity to differentiate to the tissue lineage of all three germ layers. (ca.gov)
  • Efficient Endoderm Induction from Human Pluripotent Stem Cells by Logically Directing Signals Controlling Lineage Bifurcations. (edu.au)
  • Using this type of direct conversion approach rather than making them from induced pluripotent stem cells, where they revert into a naïve state, helps to retain the epigenetic age of the patient in the generated interneurons. (lu.se)
  • We have started pre-clinical studies of transplanting cardiovascular progenitors (at different stages of development) and mature cardiomyocytes in animal models. (ca.gov)
  • NKX2-5eGFP/w hESCs for isolation of human cardiac progenitors and cardiomyocytes. (edu.au)
  • Using trophoblast stem cells (TSCs) to produce trophoblast - conditioned medium (TCM), we show that TCM profoundly slows down the cardiomyocyte differentiation dynamics and specifically delays the emergence of cardiac mesoderm progenitors. (bvsalud.org)
  • mEScell growth and differentiation on PLDLA-co-TMC membrane, demonstrated the cyto compatibility of this polymer, and provided evidence of minimal to no toxicity on the cells, allowing adhesion and cell proliferation, as well as differentiation into cardiomyocytes. (jscimedcentral.com)
  • In cardiac conditional Ezh2-knockout mice, cardiomyocyte proliferation was repressed, and the apoptosis process was induced [ 10 ]. (hindawi.com)
  • This resulted in extreme cardiomyocyte proliferation and hypertrophy - excessive growth of the individual cardiomyocytes - leading to a giant heart (cardiomegaly) that left little room for blood to enter. (eurekalert.org)
  • Notch1, Notch2, and Epstein-Barr virus-encoded nuclear antigen 2 signaling differentially affects proliferation and survival of Epstein-Barr virus-infected B cells. (tum.de)
  • It also features additional information on "organs on chips" and adult progenitor cells. (wikipedia.org)
  • VE-CADHERIN is expressed transiently in early ISL1 + cardiovascular progenitor cells and facilitates cardiac differentiation. (nih.gov)
  • The research team conducted studies on mouse models to see how efficiently c-kit-positive cardiac progenitor cells regenerate cardiomyocytes. (pennywarren.co.uk)
  • Clare's lab recently identified a population of progenitor/stem cells in mice that, on transplantation, is sufficient to generate a fully functional thymus. (eurostemcell.org)
  • Progenitor cell therapy is being investigated for the treatment of damaged myocardium resulting from acute or chronic cardiac ischemia and for refractory angina. (southcarolinablues.com)
  • For individuals who have acute cardiac ischemia who receive progenitor cell therapy, the evidence includes 2 randomized controlled trials (RCTs) with 200 patients, numerous small RCTs, and meta-analyses of these RCTs. (southcarolinablues.com)
  • Overall, this evidence has suggested that progenitor cell treatment may be a promising intervention, but robust data on clinical outcomes are lacking. (southcarolinablues.com)
  • For individuals who have chronic cardiac ischemia who receive progenitor cell therapy, the evidence includes a nonrandomized comparative trial and systematic reviews of smaller RCTs. (southcarolinablues.com)
  • Results of the nonrandomized trial are encouraging, because this is the first controlled trial that has reported a significant mortality benefit for progenitor cell treatment. (southcarolinablues.com)
  • For individuals who have refractory angina who receive progenitor cell therapy, the evidence includes phase 2 trials and a phase 3 pivotal trial. (southcarolinablues.com)
  • Additional larger trials are needed to determine whether progenitor cell therapy improves health outcomes in patients with refractory angina. (southcarolinablues.com)
  • RATIONALE: The Notch signaling pathway is important for cell-cell communication that controls tissue formation and homeostasis during embryonic and adult life, but the precise cell targets of Notch signaling in the mammalian heart remain poorly defined. (monash.edu)
  • OBJECTIVE: To investigate the functional role of Notch signaling in the cardiomyocyte compartment of the embryonic and adult heart. (monash.edu)
  • CONCLUSIONS: These results reveal a strict requirement for cell-autonomous modulation of Notch signaling during heart morphogenesis, and illustrate how the same signaling pathway that promotes congenital heart defects when perturbed in the embryo can be therapeutically redeployed for the treatment of adult myocardial damage. (monash.edu)
  • My research interests focus mainly on stem cells (embryonic, 'adult' and fetal origins) and their applications in tissue engineering, particularly the osteoblast and bone tissue. (nottingham.ac.uk)
  • Next, the team reactivated the ERBB2 protein in adult mouse heart cells, in which cardiomyocytes normally no longer divide. (eurekalert.org)
  • The question then became: If one could activate ERBB2 for just a short period in an adult heart following a heart attack, might it be possible to get the positive results, i.e., cardiac cell renewal, without such negative ones as hypertrophy and scarring? (eurekalert.org)
  • Investigation of the regenerative process through live imaging and molecular studies revealed how this happens: The cardiomyocytes "dedifferentiate" - that is, they revert to an earlier form, something between an embryonic and an adult cell, which can then divide and differentiate into new heart cells. (eurekalert.org)
  • Several clinical trials of cell-based therapy have generated enthusiasm about the potential of adult stem cells to treat heart disease. (ca.gov)
  • Literature review: This review summarizes current knowledge, barriers, and challenges in the clinical use of adult stem cells, scaffolds, and growth factors for the development and evaluation of regenerative endodontic therapies. (bvsalud.org)
  • The most valuable cells for regenerative endodontics are postnatal or adult stem cells. (bvsalud.org)
  • Based on their origin, there are two main types of stem cells - embryonic stem cells (ES cells) and postnatal or adult stem cells (AS cells). (bvsalud.org)
  • One way we did this was by converting adult fibroblasts, or skin cells, into interneurons. (lu.se)
  • Tahrir FG, Gordon J, Feldman AM, Cheung J, Khalili K, Mohseni Ahooyi T ( 2019 ) Evidence for the impact of BAG3 on electrophysiological activity of primary culture of neonatal cardiomyocytes. (multichannelsystems.com)
  • image: Two neonatal cardiomyocytes (stained red) undergoing cell division after treatment with NRG1 are shown. (eurekalert.org)
  • In the early embryonic development, abnormal expression of EZH2 impaired embryo growth and pluripotency maintenance [ 7 , 8 ]. (hindawi.com)
  • In pigs, several studies have focused on its function in early embryonic development regulation and in SCNT efficiency improvement [ 8 , 14 - 16 ]. (hindawi.com)
  • In 1993, Prof. Mummery became a group leader working on TGFβ and BMP signalling in mouse development using cardiomyogenesis and vasculogenesis in mouse and human (embryonic) stem cells as models. (wikipedia.org)
  • Embryonic stem cells (ESC) provide a great therapeutic potential for cardiac tissue. (jscimedcentral.com)
  • For this purpose, we use human stem cells that play a pivotal role in tissue development, maintenance and function. (gsi.de)
  • I returned full time to Imperial College in 1999 to take up a lectureship in cell biology and tissue engineering and was also actively involved in establishing the Tissue Engineering Centre. (nottingham.ac.uk)
  • I was also affiliated with the Wolfson Centre for Stem Cells, Tissue Engineering and Modelling (STEM), part of the Centre for Biomolecular Sciences, which is now the Biodiscovery Institute. (nottingham.ac.uk)
  • To date, a lot of EZH2 variants have been found in various cell and tissue types [ 18 - 20 ]. (hindawi.com)
  • When a heart attack strikes, heart muscle cells die and scar tissue forms, paving the way for heart failure. (eurekalert.org)
  • A new study challenges the efficacy of using stem cells to regenerate damaged heart tissue. (pennywarren.co.uk)
  • It is possible that the transplanted cells fail to physiologically couple with the host tissue, or they may modify the substrate such that a pro-arrhythmic focus is created. (ca.gov)
  • There is currently no therapy that successfully regenerates heart tissue, and this despite more than a decade of stem cell research that initially appeared to be very promising in this regard. (fondationleducq.org)
  • 3) Develop pre-clinical mammalian models of regenerative therapy through the delivery of cardiomyocyte mitogens and the engineering of cardiac tissue patches from stem cell-derived cardiomyocytes. (fondationleducq.org)
  • The key elements of tissue engineering are stem cells, morphogen, and a scaffold of extracellular matrix. (bvsalud.org)
  • Postnatal stem cells have been sourced from umbilical cord blood, umbilical cord, bone marrow, peripheral blood, body fat, and almost all body tissues, including the pulp tissue of teeth 8 . (bvsalud.org)
  • At this time I also began working with mouse and human embryonic stem cells resulting in the publication of some of the first papers describing the osteogenic differentiation of mouse ES and human cells in vitro and in vivo . (nottingham.ac.uk)
  • the EST, which assesses the effects of compounds on the differentiation of mouse embryonic stem cells into contracting cardiomyocytes, can be used to rank the potency of chemicals within a series of alkoxyacetic acid metabolites formed from The implementation of the European REACH (Registration, glycol ethers. (cdc.gov)
  • selectively promotes the differentiation of ESCs to cardiomyocytes in vitro between days 3-5. (tocris.com)
  • The influence of melatonin on the heart rhythm - An in vitro simulation with murine embryonic stem cell derived cardiomyocytes. (uni-koeln.de)
  • In vitro toxicity data of these metabolites derived in the development of validated and accepted in vitro and in silico embryonic stem cell test were used as input in the PBK model to extrapolate in vitro concentration-response curves to predicted approaches is urgently needed. (cdc.gov)
  • Promotes embryonic stem cell (ESC) differentiation into cardiomyocytes. (fishersci.com)
  • Cell therapy with embryonic stem cell-derived cardiomyocytes encapsulated in injectable nanomatrix gel enhances cell engraftment and promotes cardiac repair. (uab.edu)
  • TCM also strongly promotes the retention of pluripotency transcription factors , thereby sustaining the stem cell state of ESCs. (bvsalud.org)
  • Vascular endothelial cell (EC)-derived factors play an important role in endothelial-cardiomyocyte crosstalk and could save cardiomyocytes (CMs) from injury. (mdpi.com)
  • Our approaches provide a meaningful deconstruction of the intricate crosstalk between the embryonic and the extraembryonic compartments. (bvsalud.org)
  • Embryonic precursors of liver and heart , whilst not sharing cellular origin, develop in close proximity through a dynamic series of inductive signaling events. (xenbase.org)
  • Noninvasive detection and imaging of molecular markers in live cardiomyocytes derived from human embryonic stem cells Biophysical Journal. (nottingham.ac.uk)
  • Dr Jeffery Molkentin, study principal investigator and a cardiovascular molecular biologist and HHMI investigator at the Cincinnati Children's Heart Institute, said this effect is unlikely to be due to the generation of new contractile cells, known as cardiomyocytes. (pennywarren.co.uk)
  • Molecular endpoints indicate a role for reactive oxygen species and changes in cell membrane proteins. (nottingham.ac.uk)
  • This is an experimental treatment based on preclinical studies in rats and mice which have indicated that c-kit-positive stem cells completely regenerate myocardial cells and heart muscle. (pennywarren.co.uk)
  • METHODS AND RESULTS: Here, we report that either conditional overexpression of Notch1 intracellular domain (NICD1) or selective silencing of Notch signaling in the embryonic cardiomyocyte compartment results in developmental defects and perinatal lethality. (monash.edu)
  • In 1981, she continued with a Postdoctoral fellowship at the Hubrecht Institute (KNAW), Utrecht, working on neuroblastoma and embryonal carcinoma cells as screens for teratogens before being appointed tenured staff scientist working on developmental biology, differentiation and stem cells in 1985. (wikipedia.org)
  • We have isolated these cells and have performed detailed analysis to fully characterize their developmental potential. (ca.gov)
  • Normal developmental progression relies on close interactions between the embryonic and extraembryonic lineages in the pre- and peri- gastrulation stage conceptus. (bvsalud.org)
  • Here, we established a robust differentiation protocol of mouse embryonic stem cells (ESCs) into cardiomyocyte -containing embryoid bodies that we used to test the impact of trophoblast on this key developmental process. (bvsalud.org)
  • Next, the importance of embryonic developmental genes such as homeobox and T-box transcription factors are highlighted in conduction system development and function. (medscape.com)
  • The slow-conducting SA and AV nodes take developmental origin from the slow-conducting embryonic inflow tract and atrioventricular canal region. (medscape.com)
  • By applying TCM from various mutant TSCs, we further show that those mutations that cause a trophoblast -mediated effect on early heart development in vivo alter the normal cardiomyocyte differentiation trajectory. (bvsalud.org)
  • The Effect of Antiarrhythmic Drugs on the Beat Rate Variability of Human Embryonic and Human Induced Pluripotent Stem Cell Derived Cardiomyocytes. (uni-koeln.de)
  • It thoroughly investigated the unique ability of mouse embryonic stem cells to develop into cells of any organ ("pluripotency"), created new tools for functional genomic studies and thus provided key knowledge to understanding the commitment of cells to differentiate into cells of a particular germ layer. (europa.eu)
  • A stem cell is commonly defined as a cell that has the ability to continuously divide and produce progeny cells that differentiate (develop) into various other types of cells or tissues. (bvsalud.org)
  • Furthermore, the decreased expression of the EZH2 gene is crucial for stem cell differentiation into specific cell lineages involved in myogenesis, adipogenesis, osteogenesis, neurogenesis, and haematopoiesis [ 9 ]. (hindawi.com)
  • This figure fell below 0.08 when considering a natural process called cellular fusion - where c-kit-positive cells from the bone marrow or circulating immune system cells fuse with cardiomyocytes in the heart. (pennywarren.co.uk)
  • Conventional bone marrow-derived dendritic cells contribute to toll-like receptor-independent production of alpha/beta interferon in response to inactivated parapoxvirus ovis. (tum.de)
  • FUNGENES identified the gene subsets that are active in self-renewing cells, during the exit from pluripotency and at specific stages of mouse ES cell differentiation. (europa.eu)
  • The Trophoblast Compartment Helps Maintain Embryonic Pluripotency and Delays Differentiation towards Cardiomyocytes. (bvsalud.org)
  • Transcription factor over-expression is a proven method for reprogramming cells to a desired cell type for regenerative medicine and therapeutic discovery. (nature.com)
  • During more recent years, his reseach has been conducted in the field of regenerative medicine, focussed on antigen expression and immune recognition of human embryonic stem cells and bioartificial heart valves, the latter tissues being of animal origin. (gu.se)
  • We're a partnership of more than 400 stem cell and regenerative medicine labs across Europe, connected via research centres, consortia, networks and hubs. (eurostemcell.org)
  • Paolo De Coppi is a Consultant Paediatric Surgeon at Great Ormond Street Hospital (GOSH), and Reader and Head of Stem Cells and Regenerative Medicine at the UCL Institute of Child Health in London. (eurostemcell.org)
  • Prof. Mummery is also the co-founder of LUMC spinout Pluriomics bv (now Ncardia since September 2017) Mummery is a board member of the Hubrecht Institute (KNAW), the Netherlands Organisation for Scientific Research and the International Society of Stem Cell research (ISSCR) from which she is vice president since 2018 until 2020. (wikipedia.org)
  • EuroStemCell has key partnerships with European Union-funded stem cell research consortia who provide significant contribution to the project financially and scientifically. (eurostemcell.org)
  • He co-ordinates the EU Horizon2020 funded stem cell research consortium - INTENS which aims to aims to make a functional reconstructed bowel for people with Short Bowel Syndrome. (eurostemcell.org)
  • chick embryonic heart micromass (MM) and mouse embryonic D3 stem cells (ESD3). (nottingham.ac.uk)
  • This approach could therefore micromass test, and the embryonic stem cell test (EST) provide a means to reduce the need for animal testing in human risk assessment practices. (cdc.gov)
  • At the Stem Cell Differentiation and Cytogenetics Group, we are interested in the effects of ionizing radiation on organs such as the brain, the heart, and the lung trying to elucidate the risks for patients treated with ionizing radiation as well as for astronauts, who are inevitably exposed to ionizing radiation on their missions into space. (gsi.de)
  • Particularly, human embryonic stem cells (hES cells), which can give rise to all tissues of an organism (Figure A), are used to generate mature cells of the heart (Figure B) allowing us to study radiation/microgravity-induced cardiovascular alterations, which may occur during long-term space missions. (gsi.de)
  • Endocardium differentiation through Sox17 expression in endocardium precursor cells regulates heart development in mice. (nih.gov)
  • Chris' primary research focus is on using experimental and clinical data to predict how heart cells, the engine of every heartbeat, respond to genetic mutations and to drugs. (swansea.ac.uk)
  • As opposed to blood, hair or skin cells that can renew themselves throughout life, our heart cells cease to divide shortly after birth, and there is very little renewal in adulthood. (eurekalert.org)
  • He and his team plan to continue researching this signaling pathway to suggest ways of improving the process, which may, in the future, point to ways of renewing heart cells. (eurekalert.org)
  • Understanding the genetic pathways underlying differentiation of ES cells to somatic cells would contribute to future therapeutic strategies for degenerative diseases such as heart disease, diabetes and Parkinson's. (europa.eu)
  • Many patients have already been treated using c-kit positive stem cells, which are removed from healthy regions in a damaged heart, processed in a laboratory and then re-injected into the patients' hearts. (pennywarren.co.uk)
  • It was found that c-kit-positive cells originating in the heart generated new cardiomyocytes at a percentage (from baseline) of 0.03 or less. (pennywarren.co.uk)
  • Therefore, treatment of these cells with an antibiotic results in selection of the desired heart cells while eliminating all unwanted contaminant cells. (ca.gov)
  • We have shown engraftment of these cells into the host heart, albeit a very low efficiency. (ca.gov)
  • As such, grafted cells in the heart can also be an ectopic source of activities, promoting arrhythmic events. (ca.gov)
  • We are in the process of developing imaging modalities to investigate the extent of structural and functional integration of the transplanted cells into the host heart. (ca.gov)
  • Coxsackievirus B3 regulates T-cell infiltration into the heart by lymphocyte function-associated antigen-1 activation via the cAMP/Rap1 axis. (medscape.com)
  • The heart achieves the coordinated contraction of the atrial and ventricular chambers due to the precise timing of the cardiac conduction system (CCS), a specialized complex and heterogeneous network of cells that initiate and allow propagation of action potentials through the heart. (medscape.com)
  • The normal cardiac impulse of the vertebrate heart originates in the pacemaker cells of the sinoatrial node, located in the right atrium. (medscape.com)
  • hES cells also serve as a basis for cerebral organoid models (Figure C) to better understand e.g. the mechanisms of cognitive impairment and radiation necrosis as severe side effects of radiation therapies. (gsi.de)
  • Non-thermal disruption of β-adrenergic receptor-activated Ca2+ signalling and apoptosis in human ES-derived cardiomyocytes by microwave electric fields at 2.4 GHz. (swansea.ac.uk)
  • In this study, the interaction of mouse embryonic stem cells (mESC) with poly L-co-D, L lactic acid co-trimethylene carbonate (PLDLA-co-TMC) was assessed. (jscimedcentral.com)
  • EZH2, a methyltransferase catalyzing H3K27me3, has been abundantly studied in human and mouse embryonic development. (hindawi.com)
  • QuinoMit Q10-Fluid attenuates hydrogen peroxide-induced irregular beating in mouse pluripotent stem cell-derived cardiomyocytes. (uni-koeln.de)
  • The diabetic formula used showed an increase in DNA damage and a decline in cell migration in mouse embryonic stem cells. (nottingham.ac.uk)
  • For example, mouse epiblast -derived FGF and NODAL signals are required to maintain a stem -like state in trophoblast cells of the extraembryonic ectoderm , while visceral endoderm signals are pivotal to pattern the anterior region of the epiblast . (bvsalud.org)
  • physiologically based animals because a mouse embryonic stem cell line is used. (cdc.gov)
  • Rare mutations of ADAM17 from TOFs induce hypertrophy in human embryonic stem cell-derived cardiomyocytes via HB-EGF signaling. (cdc.gov)
  • however, restoration of CBY1 expression in tumor cells reduces BCSC and its enrichment, thus lnc408 plays an essential role in maintenance of BCSC stemness. (nature.com)
  • These results validated a different transcript in pigs and characterized its expression profile in fetal tissues of different gestation stages, which indicated that EZH2 played important roles during porcine embryonic development. (hindawi.com)
  • Thus, the appropriate expression level of the EZH2 gene is important for embryonic development. (hindawi.com)
  • However, the transcript and expression status of the EZH2 gene during porcine embryonic development remains unknown. (hindawi.com)
  • Subsequently, we detected the expression of EZH2 on mRNA level and protein level in two different embryonic development stages (65-dpc and 90-dpc) via qRT-PCR and western blots. (hindawi.com)
  • Its major objective was to produce a gene expression atlas covering the development of ES cells into all three germ layers (ecto-, meso-, and endoderm) and into somatic cells. (europa.eu)
  • These include contractile activity (morphological scoring system), cell activity, total protein content, ROS production, DNA damage, transmembrane proteins expression (connexin43, integrin β1) and stem cell migration. (nottingham.ac.uk)
  • Examination of gene expression in st. 43 AC/AE explants by RT-PCR reveals expression of liver (fabp1), intestine (fabp2), pancreas (pdia2 and pdx1 , but not ins and amy2a), thyroid/lung (nkx2-1) as well as ventricular cardiomyocyte (myl3) markers. (xenbase.org)
  • I work on various biochemical and biophysical approaches to investigate and control cell-cell, cell-scaffold interactions. (nottingham.ac.uk)
  • 3. developed new embryonic stem cell-based approaches to screening of small candidate molecules for therapeutic applications in human diseases. (europa.eu)
  • Tumor initiation, development, and relapse may be closely associated with cancer stem cells (CSCs). (nature.com)
  • Our study is aimed at providing information for understanding the biological function of EZH2 in porcine embryonic development. (hindawi.com)
  • This complex process occurs in several steps and controls the development of pluripotent cells into highly specialised cells of an organism. (europa.eu)
  • The thymus plays a central role in the development of the body's immune system as it is the main site in which T cells are generated. (eurostemcell.org)
  • The major areas of research that might have application in the development of regenerative endodontic techniques are (a) postnatal stem cells, (b) scaffold materials, (c) morphogen/growth factors, (d) implantation. (bvsalud.org)
  • The study of promoter interactions in other hESC-derived cell types may be of utility in functional investigation of GWAS-associated regions. (manchester.ac.uk)
  • All these diseases are characterised by the irreversible loss of functional cells. (europa.eu)
  • The FUNGENES project aimed to achieve a detailed basic understanding of stem cell self-renewal and differentiation. (europa.eu)
  • Mature cardiomyocytes have passed the stage of self-renewal and may pose problems due to lack of proper integration. (ca.gov)
  • This explains why researchers are now focusing attention on developing stem cell therapies using postnatal stem cells donated by the patients themselves or their close relatives. (bvsalud.org)
  • Fourier transform infrared microspectroscopy identifies early lineage commitment in differentiating human embryonic stem cells. (edu.au)
  • check the tag ADOLESCENCE HN - 2008 BX - Nutrition in Adolescence FX - Adolescent Nutrition Physiology MH - Peritoneal Stomata UI - D054048 MN - A01.047.025.600.700 MN - A10.810 MS - Natural openings in the subdiaphragmatic lymphatic plexus in the PERITONEUM, delimited by adjacent mesothelial cells. (bvsalud.org)
  • I cover mainly cell biology and physiology. (nottingham.ac.uk)
  • This research appeared in Nature Cell Biology . (eurekalert.org)
  • Combining cell biology and electrophysiology, his work has the potential to create personalized disease models for future research. (lu.se)
  • Here we examine the success rate of methods and data for differentiation by testing the ability of nine computational methods (CellNet, GarNet, EBseq, AME, DREME, HOMER, KMAC, diffTF and DeepAccess) to discover and rank candidate factors for eight target cell types with known reprogramming solutions. (nature.com)
  • Normalized area under rank recall curve values for all methods are available in Supplementary Tables 2 - 6 and for epigenomic marks of liver cells in Extended Source Data Fig. 3 . (nature.com)
  • Gene and Cell Therapy for Sinus and AV Nodal Dysfunction. (heartandcoeur.com)
  • Somatic cell therapy is viewed as a more conservative, safer approach because it affects only the targeted cells in the patient, and is not passed on to future generations. (omicsonline.org)
  • Such single gene disorders are good candidates for somatic cell therapy. (omicsonline.org)
  • ISSCR Public Service Award 2023 Elected member Academia Europaea (2015) Hans Bloemendaal Medal 2014 for innovative interdisciplinary research Hugo van de Poelgeest Prize 2014 for Animal Alternatives Elected member of the Royal Netherlands Academy of Arts and Sciences (KNAW) (elected 2010) Radcliffe Fellowship, Harvard University (2007) Royal Society Fellowship, UK (1978, 1979) Prof. Mummery is part of five different patents on differentiation of cardiomyocytes and hESC. (wikipedia.org)
  • The primitive streak gene Mixl1 is required for efficient haematopoiesis and BMP4-induced ventral mesoderm patterning in differentiating ES cells. (edu.au)
  • ERBB2 is a specialized receptor - a protein that transmits external messages into the cell. (eurekalert.org)
  • Then, the C/EBP 3â UTR was found to directly inhibit the phosphorylation activity of protein kinase CPKC in SMMC-7721, a hepatocarcinoma cell line. (omicsonline.org)
  • In MM, 6-gingerol decreased contractility, cell activity and protein content. (nottingham.ac.uk)
  • In ESD3, 6-gingerol severely affected differentiation into cardiomyocytes cell activity and protein content at both low and high concentrations. (nottingham.ac.uk)
  • G-Rg1, from ginseng, decreased contractile activity, cell activity, protein content and elevated ROS production in both systems only at high concentrations. (nottingham.ac.uk)
  • Eugenol (holy basil) at moderate to high concentrations, significantly decreased contractility, cell activity and protein content. (nottingham.ac.uk)
  • HN - 2008 BX - Granulosa Cells, Cumulus MH - Coronary Sinus UI - D054326 MN - A07.231.908.194.500 MS - A short vein that collects about two thirds of the venous blood from the MYOCARDIUM and drains into the RIGHT ATRIUM. (bvsalud.org)
  • Our data shows that cell isolation by mechanical cell sorting exerts harmful effect on the cells, compromising their viability. (ca.gov)