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  • stromal cells
  • We tested the hypothesis that the stromal cells from PZ and transitional zone (TZ) have differential effects on the ability of tumorigenesis. (jcancer.org)
  • The C-Kit inhibitor, imatinib mesylate, was administrated to confirm the effect of stromal cells on the tumorigenesis. (jcancer.org)
  • In contrast, the data was significantly lower with DU145 and stromal cells from TZ than DU145 alone. (jcancer.org)
  • These effects of the stromal cells in the PZ on DU145 cells could be blocked using imatinib mesylate. (jcancer.org)
  • in contrast, the stromal cells in the TZ inhibit it through down-regulating the expression of C-Kit. (jcancer.org)
  • proteins
  • In exponentially growing P19 embryonal carcinoma (EC) cells, WT1 mRNA and proteins were undetectable. (aacrjournals.org)
  • However, it has been demonstrated that fibroblast growth factor 2 (FGF2) maintains self-renewal of human ES or iPS cells by supporting stable expression of the extracellular matrix proteins through the activation of the PI3K/Akt pathway. (springer.com)
  • Activation of the Smad signaling pathway by bone morphogenetic proteins (BMPs) and activin/nodal has been shown to promote cardiovascular differentiation of mouse and human ES or iPS cells. (springer.com)
  • Line
  • Most human prostate cancers are adenocarcinomas, which originate from the epithelial cells that line the glands and ducts of prostate [ 2 ]. (jcancer.org)
  • CHO-Anti-Human FUT4 scFv stable cell line is clonally-derived from a CHO cell line, which has been transfected with an Anti-human FUT4 scFv gene to allow expression of the scFv. (creativebiomart.net)
  • It is an example of a cell line transfected using our proprietary CBTGS gene screening and amplification system. (creativebiomart.net)
  • What's more, down regulation of NR6A1 could reverse epithelial-to-mesenchymal transition (EMT) process in DU145 and PC3 cell lines, and the overexpression could enhance EMT process in 22RV1 cell line. (oncotarget.com)
  • Growth
  • Prevalent expression of fibroblast growth factor (FGF) receptors and FGF2 in human tumor cell lines. (wikipathways.org)
  • Once cells are thawed, it is important to dilute the cells 1:10 in growth media immediately to reduce the potentially toxic effects of the DMSO preservative on the cells.2. (creativebiomart.net)
  • Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.3. (creativebiomart.net)
  • Furthermore, the existence of variant cells creates an essential substrate for in vitro selection whereby mutations that endow cells with improved growth outcompete their normal counterparts and overtake the culture-a phenomenon termed culture adaptation (Baker et al. (springer.com)
  • However, the presence of genetic changes in hPSCs coupled with their increased growth rates is reminiscent of the defining features of cancer cells (Baker et al. (springer.com)
  • progenitor cells
  • This link between the control of DNA replication during early development and germ cell neoplasia reveals Geminin as a potential chemotherapeutic target in the eradication of cancer progenitor cells. (mdpi.com)
  • lines
  • Do Cancer Cell Lines Have Fixed or Fluctuating Stem Cell Phenotypes? (semanticscholar.org)
  • It is remarkable that permanent pluripotent stem cell lines can be derived from preimplantation embryos at all, because, in vivo, pluripotent cells of the early mammalian embryo proliferate only briefly before becoming cells with a more restricted developmental potential. (biologists.org)
  • However, this term was never adopted, and the extent to which these pluripotent stem cell lines represent any specific embryonic cell type or reflect their artificial tissue culture environment is still an open issue today - two decades later. (biologists.org)
  • 1. Remove the packaging cell lines from liquid nitrogen and carry out a quick thaw. (creativebiomart.net)
  • The predominant genetic changes found in hPSC lines involve changes in chromosome number and structure (particularly of chromosomes 1, 12, 17 and 20), reminiscent of the changes observed in cancer cells. (springer.com)
  • primordial germ
  • Mouse and human GCTs arise from primordial germ cells (PGCs), presumably from some dysfunction in their normal development shortly after they populate the genital ridge ( 20 , 21 ). (pnas.org)
  • isolation
  • SPECIFIC AIM The specific aim of this work was identification and isolation of spectra of single, living cancer stem cells, which were acquired directly from the patients' biopsies, followed by testing of their pluripotency. (semanticscholar.org)
  • origins
  • When murine PGCs are cultured in vitro under appropriate conditions, they convert to cells that closely resemble EC and ES cells, and these are known as embryonic germ cells to distinguish their origins ( 22 ). (pnas.org)
  • protein
  • Remarkably, pluripotent stem cells, like many cancer cells, depend on the Geminin protein to prevent excess DNA replication from triggering DNA damage-dependent apoptosis. (mdpi.com)
  • Here we show that Lin28, a developmentally regulated RNA binding protein, selectively blocks the processing of pri-let-7 miRNAs in embryonic cells. (sciencemag.org)