• Reduction of beta-catenin-2 function in wild-type embryos by injection of morpholino antisense oligonucleotides (MOs) specific for this gene (MO2) results in the same ventralized phenotypes as seen in ichabod embryos, and administration of MO2 to ichabod embryos increases the extent of ventralization. (biomedsearch.com)
  • beta-catenin-2 is thus specifically required for organizer formation and this function is apparently required maternally, because the ichabod mutation causes a reduction in maternal transcription of the gene and a reduced level of beta-catenin-2 protein in the early embryo. (biomedsearch.com)
  • In contrast there was no such reduction in the rhea17 embryos suggesting the G340E mutant talin protein interacts with the membrane as well as the WT protein. (nih.gov)
  • Blastomere injection of mRNA or antisense oligonucleotides has proven effective in analyzing early gene function in Xenopus.Double-targeted transfection provides a unique opportunity to monitor axon-target interaction in vivo.Finally, electroporated embryos represent a valuable source of MO-loaded or DNA transfected cells for in vitro analysis. (nih.gov)
  • The neural expression pattern of the SCL gene was highly conserved in mouse, chicken, and zebrafish embryos and the 5' region of the chicken SCL locus exhibited a striking degree of functional conservation in transgenic mice. (nih.gov)
  • The simultaneous expression of the hunchback gene in the numerous nuclei of the developing fly embryo gives us a unique opportunity to study how transcription is regulated in living organisms. (mcmaster.ca)
  • We show that the precision of the expression of the hunchback gene to measure its position along the anterior-posterior axis is low both at the boundary and in the anterior even at cycle 13, suggesting additional post-transcriptional averaging mechanisms to provide the precision observed in fixed embryos. (mcmaster.ca)
  • Finally, electroporated embryos represent a valuable source of MO-loaded or DNA transfected cells for in vitro analysis. (nih.gov)
  • Using a combination of MO1 and MO2 in wild-type embryos, or by injecting solely MO1 in ichabod embryos, we obtain expression of a wide spectrum of neural markers in apparently appropriate anteroposterior pattern. (biomedsearch.com)
  • Xenopus zinc finger transcription factor IA1 (Insm1) expression marks anteroventral noradrenergic neuron progenitors in Xenopus embryos. (ac.be)