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  • human embryos
  • The aorta-gonad-mesonephros (AGM) is a region of embryonic mesoderm that develops during embryonic development from the para-aortic splanchnopleura in chick, mouse and human embryos. (wikipedia.org)
  • During organogenesis (around the fourth week in human embryos), the visceral region of the mesoderm, the splanchnopleura, transforms into distinct structures consisting of the dorsal aorta, genital ridges and mesonephros. (wikipedia.org)
  • This is the same case in human embryos, where they are first detected at day 27 in the aorta gonad mesonephros region, expand rapidly at day 35, then disappear at day 40. (wikipedia.org)
  • activity
  • Furthermore, isolated organ cultures of the AGM from mice embryos can autonomously initiate hematopoietic stem cell activity, without influence from the yolk sac or liver. (wikipedia.org)
  • zebrafish
  • In zebrafish, the initial embryo-wide domain of BMP signaling is refined into a morphogenetic gradient following activation dorsally of a maternal Wnt pathway. (nih.gov)
  • Zebrafish, specifically at embryo stage, showed suitable in vivo model for monitoring PAHs exposure to hematopoietic tissue and other organs. (nih.gov)
  • Zebrafish embryos homozygous for the masterblind (mbl) mutation exhibit a striking phenotype in which the eyes and telencephalon are reduced or absent and diencephalic fates expand to the front of the brain. (zfin.org)
  • Two species of fish, zebrafish and medaka, are most commonly modified because they have optically clear chorions (shells), develop rapidly, the 1-cell embryo is easy to see and micro-inject with transgenic DNA, and zebrafish have the capability of regenerating their organ tissues. (wikipedia.org)
  • Embryonic
  • We have isolated a recessive dorsalizing maternal-effect mutation disrupting the gene encoding Integrator Complex Subunit 6 (Ints6).Limiting Nodal signaling or restoring BMP signaling restores wild-type patterning to affected embryos.Our results are consistent with a novel role for Ints6 in restricting the vertebrate organizer to a dorsal domain in embryonic patterning. (nih.gov)
  • However, a drawback of the mouse model is that embryonic heart rhythm is difficult to study in live embryos due to their intra-uterine development. (nih.gov)
  • We present a new model, the fruit fly Drosophila embryo offering the opportunity for rapid, inexpensive and detailed analysis of CNTs toxicity during embryonic development. (nih.gov)
  • dorsal
  • The accumulation of β-catenin in nuclei on the dorsal side of the embryo then leads to repression of BMP signaling dorsally and the induction of dorsal cell fates mediated by Nodal and FGF signaling. (nih.gov)
  • Due to widespread de-repression of dorsal organizer genes, embryos from mutant mothers fail to maintain expression of BMP ligands, fail to fully express vox and ved, two mediators of Wnt8a, display delayed cell movements during gastrulation, and severe dorsalization. (nih.gov)
  • Consistent with radial dorsalization, affected embryos display multiple independent axial domains along with ectopic dorsal forerunner cells. (nih.gov)
  • It is also expressed in the pole cells and in ectodermally derived tissues, including the epidermis.Consistent with this latter expression, mbc mutant embryos exhibit defects in dorsal closure and cytoskeletal organization in the migrating epidermis.Both the mesodermal and ectodermal defects are reminiscent of those induced by altered forms of Drac1 and suggest that mbc may function in the same pathway. (nih.gov)
  • Consistent with this latter expression, mbc mutant embryos exhibit defects in dorsal closure and cytoskeletal organization in the migrating epidermis. (nih.gov)
  • Note the midgut constrictions in C and the absence of these constrictions in D. (E and F) Dorsal vessel of stage 17 embryos. (nih.gov)
  • Dorsal/ventral patterning of the sea urchin embryo depends upon the establishment of a Nodal-expressing ventral organizer. (nih.gov)
  • It contains the dorsal aorta, genital ridges and mesonephros and lies between the notochord and the somatic mesoderm, extending from the umbilicus to the anterior limb bud of the embryo. (wikipedia.org)
  • elegans
  • Those of the two-cell stage embryos of C. elegans exhibit this pattern. (biomedsearch.com)
  • Moreover, in 15% of dhc-1 (RNAi) embryos, centrosomes failed to remain in proximity of the male pronucleus.Therefore, cytoplasmic dynein is required for multiple aspects of MTOC positioning in the one cell stage C. elegans embryo.In conjunction with our observation of cytoplasmic dynein distribution at the periphery of nuclei, these results lead us to propose a mechanism in which cytoplasmic dynein anchored on the nucleus drives centrosome separation. (nih.gov)
  • amino acid
  • For this purpose we used mbcF12.7, since the protein is truncated at amino acid 492, and analyzed embryos that were genetically mbcF12.7/Df(3R)mbc-30. (nih.gov)
  • Here we show that mbl(-/-) embryos carry an amino-acid change at a conserved site in the Wnt pathway scaffolding protein, Axin1. (zfin.org)
  • mutant
  • In contrast there was no such reduction in the rhea17 embryos suggesting the G340E mutant talin protein interacts with the membrane as well as the WT protein. (nih.gov)
  • We found that injection of as little as 0.7 picograms (pg) of lft1 mRNA (Figure 6A, +0.7× middle row), a dose that only weakly perturbs WT embryos (Figure 6A, Minor head defects), could restore WT or nearly WT patterning in 33% of mutant embryos. (nih.gov)
  • Injection of 1 pg of lft1 mRNA (Figure 6A, +1× middle row) restored WT patterning in a larger fraction of mutant embryos and suppressed mesendoderm formation (oep-like) in 20% of mutant embryos. (nih.gov)
  • Thus, suppression of Nodal signaling can restore the balance between axial and non-axial fate specification in mutant embryos, similarly to restoring BMP signaling. (nih.gov)
  • Given the broad expression pattern of mbc, it was of interest to examine mbc mutant embryos for defects in other tissues. (nih.gov)
  • Nodal
  • Limiting Nodal signaling or restoring BMP signaling restores wild-type patterning to affected embryos. (nih.gov)
  • Remarkably, impairing hbox12 function, either in a spatially-restricted sector or in the whole embryo, specifically rescues nodal transcription in Trichostatin-A-treated larvae. (nih.gov)
  • larvae
  • If embryos develop into healthy larvae, the larvae will hatch out of the egg shell. (nih.gov)
  • Microbiota may be passed on to offspring via bacteriocytes associated with the ovaries or developing embryo, by feeding larvae with microbe-fortified food, or by smearing eggs with a medium containing microbes during oviposition. (wikipedia.org)
  • Sequence
  • Time-lapse DIC microscopy recordings of wild-type (A-D) and dhc-1 (RNAi) embryos (E-H). Time elapsed since the beginning of the sequence is displayed in minutes and seconds in each image. (nih.gov)
  • Injection
  • Blastomere injection of mRNA or antisense oligonucleotides has proven effective in analyzing early gene function in Xenopus.Double-targeted transfection provides a unique opportunity to monitor axon-target interaction in vivo.Finally, electroporated embryos represent a valuable source of MO-loaded or DNA transfected cells for in vitro analysis. (nih.gov)
  • B, C) Live snapshots of embryos injected with 1 mg/ml DiI in DMSO (red, B, DMSO/DiI) or 1 mg/ml DiI labelled MWCNTs in DMSO (red, C, MWCNT-DiI) immediately after injection into an embryo with microtubules labelled by GFP (green, GFP-jupiter). (nih.gov)
  • D, E) Live confocal section of the same embryo 8 h (D) and 15 h (E) after injection. (nih.gov)
  • Hatching rate of embryos injected with water (blue, injection control), 10% DMSO (yellow, vehicle control), 1 mg/ml MWCNT in 10% DMSO/water (black, MWCNT/DMSO, see also Experimental Section) and 1 mg/ml MWCNT in 10% DMSO labelled with DiI (red, MWCNT/DiI/DMSO). (nih.gov)
  • indicate
  • Further analyses by comparative 2D-TLC with adult fish and chick embryo brains indicate the pentasialilated ganglioside GP1c as the possible structure of XI. (biomedsearch.com)
  • tissues
  • At least two other more polar (presumably richer in sialic acid) bands are often visible under XI, both in embryos and in brain and spinal cord tissues of adult Xenopus. (biomedsearch.com)
  • stage
  • A and B) Somatic muscle pattern of stage 16 embryos. (nih.gov)
  • A) P. lividus embryos cultured in the absence or in the presence of either TSA or VPA at the indicated dosages, and observed at the early blastula stage. (nih.gov)
  • B) Northern blot analysis of total RNA isolated from embryos at the early blastula stage treated or not treated with TSA or VPA, and probed with an antisense 32P labelled RNA against the hbox12 transcript. (nih.gov)
  • D) Spatial distribution of the hbox12 transcripts in control and TSA-treated embryos at the early blastula stage, revealed by chromogenic WMISH. (nih.gov)
  • Analysis with time-lapse differential interference contrast microscopy and indirect immunofluorescence revealed that pronuclear migration and centrosome separation failed in one cell stage dhc-1 (RNAi) embryos. (nih.gov)
  • severe
  • These embryos exhibited the severe somatic muscle phenotype previously reported (Rushton et al. (nih.gov)
  • granules
  • In addition to modifying the oocyte's extracellular matrix and establishing a block to polyspermy, the exocytosis of cortical granules may also contribute towards protection and support of the developing embryo during preimplantation. (wikipedia.org)
  • adult
  • Alternative splicing is temporally and spatially regulated in embryo and adult. (nih.gov)
  • possibility
  • Therefore, Gsk3 activity may be decreased in mbl(-/-) embryos and in support of this possibility, overexpression of either wild-type Axin1 or Gsk3beta can restore eye and telencephalic fates to mbl(-/-) embryos. (zfin.org)
  • significantly
  • This method significantly decreases the time, effort, microscopy, and embryo costs by a factor of the number of specimens imaged per session, typically six. (biomedsearch.com)
  • early
  • Tracing individual cell positions can rapidly become a large-scale problem because cell numbers often grow exponentially in the early embryo. (biomedsearch.com)
  • We show that injected DiI labelled multi-walled carbon nanotubes (MWCNTs) become incorporated into cells in early Drosophila embryos, allowing the study of the consequences of cellular uptake of CNTs on cell communication, tissue and organ formation in living embryos. (nih.gov)
  • wild-type
  • A and E) In both wild-type and dhc-1 (RNAi) embryo, the male pronucleus is apposed to the posterior cortex (A and E, rightmost arrow). (nih.gov)
  • Note the pseudocleavage furrow in the middle of both wild-type and dhc-1 (RNAi) embryos. (nih.gov)
  • shape
  • We are also interested in morphogenesis that provides shape to the developing embryo. (yale.edu)
  • This study demonstrates that heterogeneity in surface tension results in significant deviations of cell behavior compared to simple soap bubble models, and thus must be taken into consideration in understanding cell shape within embryos. (biomedsearch.com)
  • analysis
  • Finally, electroporated embryos represent a valuable source of MO-loaded or DNA transfected cells for in vitro analysis. (nih.gov)
  • control
  • Top row shows uninjected p18ahub control clutch for each experiment, middle row indicates p18ahub embryos injected with mRNA, and bottom row is WT embryos injected with mRNA. (nih.gov)
  • Female
  • In contrast, there are five female pronuclei in the dhc-1 (RNAi) embryo (E, arrows towards the left point at three that are visible in this focal plane). (nih.gov)
  • Female pronuclei in some dhc-1 (RNAi) embryos were located towards the middle of the embryo (not shown). (nih.gov)
  • In contrast, neither male nor female pronuclei migrate in the dhc-1 (RNAi) embryo. (nih.gov)
  • brain
  • This procedure can be used to electroporate separate regions of the CNS in the same embryo allowing separate manipulation of growing axons and their intermediate and final targets in the brain. (nih.gov)
  • f: Wholemount brain preparation from an electroporated embryo showing different axon tracts. (nih.gov)
  • cell division
  • At these dosages, that are commensurate with effective doses determined in studies in mammalian systems [39, the rate of cell division was not altered, and embryos cleaved synchronously with respect to untreated controls (Fig 1A). (nih.gov)
  • cells
  • SOX1 expression is restricted to neuroectoderm by proliferating progenitor cells in the tetrapod embryo. (wikipedia.org)
  • visible
  • In the dhc-1 (RNAi) embryo, no bipolar structure is visible after nuclear envelope breakdown. (nih.gov)