• Method: To facilitate the analysis of endogenous bioelectric signaling and the exploitation of voltage-based cellular controls in synthetic bioengineering applications, we sought to understand the conditions under which somatic cells can stably maintain distinct resting potential values (a type of state memory) Using the Channelpedia ion channel database, we generated an array of amphibian oocyte and mammalian membrane models for voltage evolution. (tufts.edu)
  • Results: We find that typical mammalian models and amphibian oocyte models exhibit bistability when expressing different ion channel subsets, with either persistent sodium or inward-rectifying potassium, respectively, playing a facilitative role in bistable memory formation. (tufts.edu)
  • Abstract Mammalian oocyte is a highly differentiated cell which gives rise to an embryo after fertilization. (cuni.cz)
  • However, not much is known about mRNA localization and translation in the mammalian oocyte and early embryo. (cuni.cz)
  • The aim of my thesis was to determine the localization of transcripts and components of translational machinery in the mammalian oocyte and embryo and to uncover the mechanisms of spatiotemporal regulation of translation as a prerequisite for correct oocyte and embryo development. (cuni.cz)
  • Taken together our results support the notion that asymmetric localization of RNA determines the timing and localization of translation in mammalian oocyte. (cuni.cz)
  • We here review major concerns about mitochondrial bioenergetic function and morphology as well as their involvement in oocyte and early embryo development. (embj.org)
  • Quantitative analysis showed statistically significant differences during oocyte maturation and early embryo development. (cf.ac.uk)
  • WIKIMEDIA, NATIONAL INSTITUTES OF HEALTH Andrzej Tarkowski-considered by many to be the "father of modern mammalian embryology," according to an obituary published in Nature Cell Biology this week (November 29)-passed away on September 23 at age 83. (the-scientist.com)
  • One of the most salient findings from our results is that the donor cell-cycle stage critically affects the chromatin structure and development of nuclear transplant embryos when the nuclei from donor embryos were transferred to enucleated oocytes. (nii.ac.jp)
  • Cloning experiments of the protein revealed that it is a novel pre-mRNA splicing factor in mammals, and immunofluorescense experiments revealed that it is also stage -specifically accumulated in the nuclei of late 1-cell mouse embryos. (nii.ac.jp)
  • Denker, H.-W. Self-Organization of Stem Cell Colonies and of Early Mammalian Embryos: Recent Experiments Shed New Light on the Role of Autonomy vs. External Instructions in Basic Body Plan Development. (mdpi.com)
  • In the second experiments, we found that nitric oxide, a free radical molecule acts as a regulator of development, especially transition of 2-cell embryos to the 4-cell stage and formation of blastocysts. (nii.ac.jp)
  • Similar experiments in chicken and rabbit embryos subsequently revealed groups of cells with similar instructive potential. (biologists.org)
  • Importantly, C. H. Waddington expanded the notion of the organizer to birds and mammals in experiments in which he transplanted a piece from the leading edge of the primitive streak of chicken, duck and rabbit embryos into early chicken embryos and observed a duplication of the anteroposterior axis of the host ( Waddington, 1932 , 1954 ). (biologists.org)
  • Prior to the nuclear transfer experiments, Willadsen had developed methods for freezing sheep and cow embryos, and embryo manipulation methods for producing genetically identical animals, primarily identical twins in sheep, cattle, pigs, and horses, and for producing mammalian chimaeras, including interspecies chimaeras. (wikipedia.org)
  • Tarkowski was the first to propose the inside-outside hypothesis, which states that the inner cells of an embryo give rise to the embryo proper while the outer cells become the placenta. (the-scientist.com)
  • Effect of ionizing radiations, including radioisotopes, on the placenta and embryo. (wikipedia.org)
  • These two vitrification solutions gave not only a high in-vitro survival for mouse and cattle embryos, but also resulted in many offspring, both mice and calves, after the transfer of vitrified-warmed embryos to recipient animals. (nii.ac.jp)
  • Targeted disruption of genes in the mouse that result in failure of the emergence or maturation of the primitive erythroid lineage leads to early fetal death, indicating that the primitive erythroid lineage is necessary for survival of the mammalian embryo. (ehu.es)
  • Following the observation of the expanding actin rings, we realized just how beautiful and intricate the coordination between all the outer cells of the embryo is," Biro tells The Scientist . (the-scientist.com)
  • While it was thought for over a century that primitive erythroid cells were uniquely nucleated mammalian red cells, it is now recognized that they, like their definitive erythroid counterparts, enucleate to form reticulocytes and pyrenocytes. (ehu.es)
  • This surprising finding indicates that the primitive erythroid lineage is indeed mammalian, rather than non-mammalian, in character. (ehu.es)
  • ZIP ZIP: After cells in the developing embryo divide, microtubules at the dividing poles of each cell pull actin proteins into a ring. (the-scientist.com)
  • Biro and his colleagues used advanced imaging techniques to study live, fully intact mouse embryos as they developed in a Petri dish and found that rings of the protein actin form across the ball of cells and help to zipper the embryo closed in a multistep process. (the-scientist.com)
  • The concept was first introduced by Spemann and Mangold, who showed that there is a cellular population in the newt embryo that elicits the development of a secondary axis from adjacent cells. (biologists.org)
  • In 1984, at the British Agricultural Research Council's Institute of Animal Physiology, Cambridge, he successfully used cells from early embryos to clone sheep by nuclear transfer. (wikipedia.org)
  • Our strategy has been to use electroporation (EP) of plasmid DNA following whole-embryo culture (WEC). (nih.gov)
  • This book pulls together the full range of cell culture, biochemical, microscopic, and genetic techniques to study the early mammalian embryo. (oup.com)
  • For the human embryo, this represents the first two months of intrauterine development preceding the stages of the Fetus . (jove.com)
  • These data show that adenosine and A 1 ARs potently regulate mammalian heart rates via multiple effector systems at very early stages of prenatal development. (elsevier.com)
  • Not only does this reveal the 'birth' of cytoskeleton-bound cell-cell junctions in the early embryo, but also identifies cellular processes likely to be unique to these early stages of development which could cause infertility or early embryo loss if defective," Galea writes in an email to The Scientist . (the-scientist.com)
  • Publications] SEIKE,N: 'Development of frozen-thawed demi-embryos and production of identical twin calves of different ages. (nii.ac.jp)
  • Furthermore, because of the close relationship between ERV activation and ZGA, exploring the regulatory mechanism underlying ERV activation may also shed more light on the enigma of SCNT embryo development in model animals. (mdpi.com)
  • This paper uses embryos from the Harvard Embryological Collection (H.E.C.) and Carnegie Collection . (edu.au)