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CryopreservationVitrificationCryoprotective AgentsPropylene GlycolEthylene GlycolEmbryo, MammalianEmbryo TransferFertilization in VitroTissue PreservationReproductive Techniques, AssistedOocyte RetrievalSemen PreservationFreezingOocytesBlastocystPregnancyPregnancy RateFertilityOvulation InductionFertility PreservationDimethyl SulfoxideInfertility, FemaleEmbryo Culture TechniquesEmbryonic DevelopmentEmbryo ImplantationMorulaEmbryo, NonmammalianCleavage Stage, OvumSpermatozoaOvaryTissue SurvivalSperm MotilityThermal ConductivityPropylene GlycolsFreeze SubstitutionChick EmbryoCell SurvivalOvarian FollicleTissue BanksOrgan PreservationEthylene GlycolsBlastomeresSperm Injections, IntracytoplasmicTrehaloseSperm BanksEmbryonic and Fetal DevelopmentIceDry IceZygoteGlycerolSucroseMice, Inbred ICRSingle Embryo TransferLive BirthFertilizationZona PellucidaRewarmingOocyte DonationInfertilityPregnancy OutcomeTime FactorsTissue Culture TechniquesBlood PreservationSemen AnalysisIn Vitro Oocyte Maturation TechniquesButylene GlycolsFetal ViabilityCulture TechniquesEctogenesisHEPESMetaphaseSperm RetrievalAsepsisParthenogenesisNitrogenReproductive TechniquesCattleSus scrofaCell Culture TechniquesAquaporin 3Cold TemperatureSolutionsPrimary Ovarian InsufficiencyMice, Inbred CBASpecimen HandlingPrenatal InjuriesTemperatureCulture MediaOogenesisEmbryo LossGene Expression Regulation, DevelopmentalCumulus CellsSemenEgg YolkHot TemperatureTromethamineFicollEmbryo DispositionOsmosisRaffinose
Oocytes and embryosBlastocystSperm cryopreservationEmbryonicMouse EmbryosImproved embryo selectionGametes and embryosWoman's uterusOocyte vitrificationFertility preservationEggsTrophectoderm biopsySemenFrozenBiopsyFreezingLaboratoryBlastocystsEthylene glycolProcedureMethodsViabilityHormonalOvarian1998ConventionalDevelopmental competenceOutcomesFertilizationImplantStrawReproductive technologiesUndergoneTransferProcessInfertilityIncubationTemperatureHigh survivalPatient undergoesGeneticDevelopmentHumanPregnancyCPAsMesenchymalCryotoleranceFreezeCryobiology
Oocytes and embryos6
  • EN] Assisted reproduction technologies require ovarian stimulation to increase the number of oocytes and embryos. (upv.es)
  • Cryotec is an innovative method and product line developed by Dr. Masashige Kuwayama and REPROLIFE Inc. for the vitrification and warming of oocytes and embryos. (reprolife.jp)
  • this means that oocytes and embryos of all stages can be treated using the same protocol, leading to greater efficiency and convenience. (reprolife.jp)
  • Cryotec is a safe and effective method even for lower-grade oocytes and embryos that would be difficult to vitrify using traditional methods. (reprolife.jp)
  • Cryotec can be used to safely vitrify and warm oocytes and embryos that are sensitive due to the patient's age, constitution, or medical conditions. (reprolife.jp)
  • His advancements in solution composition, device design, and simplified protocols significantly improve success rates for oocytes and embryos. (reprolife.jp)
Blastocyst6
  • Does vitrification alter methylation pattern of OCT4 promoter in rabbit late blastocyst? (upv.es)
  • After vitrification-warming process, their survival and blastocyst formation rates were examined. (ecerm.org)
  • RESULTS: Survival and blastocyst formation rates of vitrified embryos were higher in EG+DMSO group than those in EG only. (ecerm.org)
  • Established in response to a need for in-house mouse services, the LUTCF provides expertise in cryopreservation of embryos by IVF or natural matings, sperm cryopreservation, rederivation services, strain expansion by IVF, ES morula/blastocyst injections, pronuclear DNA microinjections, and injection of CRISPR edited DNA. (lu.se)
  • Additionally for embryo freezing, male partner's sperm is required for fertilisation purpose and resulting embryos of either a day 3 embryo or blastocyst stage (day 5 or day 6) will be frozen for future use. (sunfert.com)
  • Non-male factor infertility couples with SDF ≥30% have shown lower rates of normal cleavage speed, high-quality embryos at day 3, blastocyst development, blastocyst quality, and implantation in intracytoplasmic sperm injection (ICSI) cycles [ 7 ]. (ecerm.org)
Sperm cryopreservation2
Embryonic6
Mouse Embryos4
  • Effect on Survival and Developmental Competence of Vitrified Mouse Embryos Using Various Cryoprotectants and Cooling Speeds. (ecerm.org)
  • METHODS: Vitrification of mouse embryos was performed with EM grid using liquid nitrogen (LN2) or SN2 and different composition of CPAs, ethylene glycol (EG) and dimethylsulfoxide (DMSO). (ecerm.org)
  • SN2 can generate high survival and developmental rate of vitrified/warmed mouse embryos when a lower concentration of CPA was applied. (ecerm.org)
  • This is currently the most popular method for the generation of targeted knock-out and knock-in models by ES-cell injections into 8-cell morulae and/or blastocysts of mouse embryos. (lu.se)
Improved embryo selection1
Gametes and embryos1
  • It is easier to handle gametes and embryos when the workspace is organized rationally with workstations and equipment. (medfertility.com)
Woman's uterus2
  • The embryos are frozen and can subsequently be thawed and implanted in a woman's uterus. (icloudhospital.com)
  • One (or sometimes two) of the embryos are then transferred back into the woman's uterus. (cnyfertility.com)
Oocyte vitrification1
  • Another drawback of oocyte vitrification is diminished developmental potential because of the decreased quality of embryos after warming. (molcells.org)
Fertility preservation6
Eggs11
  • Cryopreservation of mammalian oocytes has been successfully applied for long-term storage of surplus eggs in mammalian assisted reproductive technology (ART), including in humans. (molcells.org)
  • Embryo cryopreservation entails in vitro fertilization , a method in which eggs are extracted from a woman's ovary and joined with sperm in a laboratory to generate embryos. (icloudhospital.com)
  • These treatments fertilize eggs with sperm and can result in the formation of additional embryos. (icloudhospital.com)
  • Because frozen embryos include younger eggs, there is a lower chance of pregnancy problems if a woman decides to have a child later in life. (icloudhospital.com)
  • A transgender man (transitioning to male) may also preserve eggs or embryos before beginning hormone therapy or undergoing gender affirmation surgery. (icloudhospital.com)
  • Egg freezing also known as oocyte cryopreservation, or vitrification is a process in which women's eggs are extracted, frozen, and stored as a way to preserve a woman's reproductive potential in reproductive age. (fertilityzilla.com)
  • The eggs are freezed by the vitrification method (fast freezing) and they are kept in liquid nitrogen containers until the patient decides to have a baby but for a maximum of only 5 years as per regulations. (bebekistiyorum.com)
  • In addition to this, she has trained over 500 embryologists from over 18 countries in vitrification for eggs and embryo cryopreservation. (todaynewsspot.com)
  • For both egg and embryo cryopreservation, hormonal therapy has to be administered to stimulate the ovaries to produce as many eggs as possible. (sunfert.com)
  • The incubation component holds the sperm, eggs, and developing embryos directly. (cnyfertility.com)
  • The idea here is to develop as many high quality eggs as possible with the hopes that more more eggs = more embryos = higher chance of a live birth resulting from the INVOcell treatment cycle. (cnyfertility.com)
Trophectoderm biopsy1
  • 35, no prior miscarriage) and normal karyotype seeking elective SET were prospectively randomized into two groups: In Group A, embryos were selected on the basis of morphology and comprehensive chromosomal screening via aCGH (from d5 trophectoderm biopsy) while Group B embryos were assessed by morphology only. (biomedcentral.com)
Semen2
  • Semen, embryos, oocytes, somatic cells, nuclear DNA, and other types of biomaterial such as blood and serum can be stored using cryopreservation, in order to preserve genetic materials. (wikipedia.org)
  • Further investigation of these areas using the available breakthrough molecular tools such as omics technologies is therefore proposed to improve, optimize, or even predict the overall quality of frozen-thawed ruminant semen towards reproductive efficiency. (tci-thaijo.org)
Frozen10
  • A fertilized egg is frozen during embryo cryopreservation. (icloudhospital.com)
  • The couple can utilize the frozen embryos to conceive another child later, or they can be stored and donated to someone else through a donor program. (icloudhospital.com)
  • The number of embryos that will be frozen. (icloudhospital.com)
  • The online Alcor library contains articles detailing repair of cryonics patients by nanorobots at cryogenic temperature, in particular, "A Cryopreservation Revival Scenario using Molecular Nanotechnology" by Ralph Merkle and Robert Freitas as well as "'Realistic' Scenario for Nanotechnological Repair of the Frozen Human Brain. (biostasis.com)
  • Although aCGH followed by frozen embryo transfer has been used to screen at risk embryos (e.g., known parental chromosomal translocation or history of recurrent pregnancy loss), this is the first description of aCGH fully integrated with a clinical IVF program to select single blastocysts for fresh SET in good prognosis patients. (biomedcentral.com)
  • When results became available, frozen embryo transfer (FET) was subsequently arranged so that only euploid embryo(s) were transferred. (biomedcentral.com)
  • In 1972, mice embryos were frozen first and this was followed by the freezing of human embryos. (bebekistiyorum.com)
  • The total number of embryo transfers was stratified further into fresh and frozen embryo transfers. (ecerm.org)
  • However, in the frozen embryo transfers, there were no significant differences in clinical outcomes between the two groups. (ecerm.org)
  • All remaining embryos are frozen using a flash freezing technology called vitrification . (cnyfertility.com)
Biopsy1
  • In collaboration with molecular genetic laboratories including Reprogenetics, Genesis Genetics, Reproductive Genetics Institute and others, we have been performing embryo biopsy and preimplantation genetic screen (PGS) for spontaneous abnormal chromosomes (aneuploidies), as well as preimplantation genetic diagnosis (PGD) of specific genetic mutation on human embryos since 2004. (familyfertility.com)
Freezing11
Laboratory3
  • In vitro fertilization (IVF) involves combining an egg and sperm within a laboratory setting to create an embryo. (ivf.net)
  • We incorporate the state-of-the-art equipment and the latest technologies in our assisted reproductive laboratory to allow every embryo to develop to its fullest potential. (familyfertility.com)
  • Fluctuations in temperature and pH (acidity), along with high (20%) oxygen concentration as found in ambient air are known to adversely affect the growth of human embryos in the laboratory. (familyfertility.com)
Blastocysts2
  • Before starting the vitrification procedure, in order to reduce the negative effect of the blastocoel, expanded blastocysts should be collapsed by reducing artificially with a glass pipette the volume of the blastocoel. (planer.com)
  • Using an attenuated pipette or an equally suitable device, deposit maximum 2 blastocysts in a volume of approximately 0.3 μl of VitriStore Freeze 2 in the gutter of the tip of the vitrification straw. (planer.com)
Ethylene glycol1
  • GM501 VitriStore Freeze/VitriStore Thaw are DMSO/ethylene glycol based vitrification media that also contain PBS, sucrose, Ficoll and human serum albumin (10-20 g/Liter). (planer.com)
Procedure6
  • Once the procedure is successful the developed embryo is implanted in the uterus of the woman. (fertilityzilla.com)
  • Their combined experience guarantees that every step of the IVF procedure, from conception to embryo transfer, is carefully handled and observed. (medfertility.com)
  • What happens during the Ovarian Tissue Cryopreservation procedure? (sunfert.com)
  • A procedure summary letter with a copy of the signed ovarian tissue cryopreservation form will be given to the patient. (sunfert.com)
  • We strongly advice to read through all the steps of the vitrification/warming procedure before starting the procedure. (planer.com)
  • Conveniently place the separate parts of the vitrification device on the workbench for easy access later in the procedure. (planer.com)
Methods2
  • Introduced by Professor Gábor Vajta in 1998, OPS is recognized as one of the best high-rate cooling-warming vitrification methods, widely respected for its efficiency, practicality and versatility. (ivf.net)
  • Assist in the development and optimization of protein purification methods to improve efficiency and yield. (irvinesci.com)
Viability1
  • When 50% of embryonal cells lose viability, the remaining cells can survive and maintain the viability of the embryo, establishing pregnancy. (bebekistiyorum.com)
Hormonal1
  • At present, the only possibility to maintain fertility as well as hormonal cyclicity and endocrinological function is the cryopreservation and autologous re-transplantation of ovarian cortical fragments taken before chemotherapy or radiotherapy. (biomedcentral.com)
Ovarian2
  • In these circumstances, a treatment called ovarian tissue cryopreservation can be offered to protect their future fertility. (sunfert.com)
  • To safeguard fertility window of cancer patients, cryopreservation of human ovarian tissue has been increasingly applied worldwide, especially for young girls and women who cannot delay their cancer treatment. (sunfert.com)
19981
  • Our laboratories have been equipped with clean room technologies using high efficiency purification of air (HEPA) system since 1998. (familyfertility.com)
Conventional1
  • The treatment process using INVOcell is very similar to conventional IVF, only fertilization and subsequent early embryo development happens inside the INVOcell device (rather than an embryology lab) which is then placed inside the woman's vagina for a brief incubation period spanning a few days. (cnyfertility.com)
Developmental competence1
  • Levels of maturation-promoting factor (MPF) in oocytes decline after vitrification, and this decline has been suggested as one of the main causes of low developmental competence resulting from cryoinjury. (molcells.org)
Outcomes1
Fertilization2
Implant3
  • After a brief incubation period, the embryo is placed into the uterus, where it may implant and initiate a pregnancy. (ivf.net)
  • When an embryo does not implant, the lining sheds and is released through the vagina, and a women's period occurs. (cnyfertility.com)
  • In this case, even though the egg-sperm binding does occur, the resulting embryo is unable to implant or cannot develop till the end of the pregnancy, that is, delivery. (invitra.com)
Straw6
  • The Open Pulled Straw (OPS) was the first purpose designed tool for ultra-rapid vitrification. (ivf.net)
  • The first open vitrification technology confirmed by independent experts to eliminate the danger of cross-contamination during storage by sealing the OPS into a pre-cooled container straw. (ivf.net)
  • Uniquely suited for cryopreservation of biopsied or zona-free embryos due to the protection provided by the straw, the defined solution volume, the lack of adhesion to surfaces and trauma-free loading/ expelling. (ivf.net)
  • Place the vitrification straw in the outer sheath and seal it as indicated in the instructions for use of the vitrification device. (planer.com)
  • Remove the vitrification straw from the outer sheath as indicated in the instructions for use of the vitrification device. (planer.com)
  • Immediately plunge the vitrification straw into pre-heated Vitri-Store Thaw Medium 1 (37°C) and leave in Thawing 1 Medium for 3 minutes. (planer.com)
Reproductive technologies1
  • Despite the limitations, embryo transfer and related reproductive technologies hold promise as a means to accelerate genetic improvement and to take advantage of superior breeding sows, to break the link between health status of customer and source herds, to eradicate disease from a swine herd, and to facilitate inexpensive transport of genetic material around the world. (veteriankey.com)
Undergone1
  • Cryopreservation has undergone tremendous advances and is widely used in animal production based on decades of study of cellular permeability, freezability and empirical generalization. (engineering.org.cn)
Transfer5
  • Single embryo transfer (SET) remains underutilized as a strategy to reduce multiple gestation risk in IVF, and its overall lower pregnancy rate underscores the need for improved techniques to select one embryo for fresh transfer. (biomedcentral.com)
  • Despite a welcome trend in increased acceptance and utilization of elective SET treatment in some groups [ 4 ], most IVF cycles continue to involve two or more embryos for transfer. (biomedcentral.com)
  • Experience in management of the infertility couple, oocytes retrieval and Embryo Transfer. (generapma.it)
  • 1 Until now, however, the swine industry has never successfully implemented a commercial viable embryo transfer process as an alternative method of improving hog genetics. (veteriankey.com)
  • Embryo Transfer: The developed embryos are removed form the INVOcell device. (cnyfertility.com)
Process3
  • What happens before Embryo Cryopreservation process? (icloudhospital.com)
  • Business Process Automation is the latest process that is emerging in the business landscape when it comes to increasing business efficiency and agility. (todaynewsspot.com)
  • The efficiency of this process is therefore increased by placing three such PCO chambers sequentially in a single frame, thus increasing the 'dwelling time' of the airstream through the PCOC™3 equipment. (planer.com)
Infertility1
Incubation1
Temperature2
  • The temperature at which the solidification (vitrification) occurs is called the glass transition temperature (Tg). (biostasis.com)
  • Using pre-mix certified gas with low (6%) oxygen content, table top incubators provide a more embryo friendly environment with a more rapid temperature recovery, tighter pH control, and lower oxygen toxicity than traditional large box incubator. (familyfertility.com)
High survival2
  • It features simplified protocols, embryologist-friendly product designs, and focuses on achieving high survival rates in cryopreservation. (reprolife.jp)
  • His unwavering dedication drives innovation for high survival rates and stress-free vitrification for embryologists. (reprolife.jp)
Patient undergoes1
  • Multiple embryos are frequently generated when a patient undergoes IVF. (icloudhospital.com)
Genetic3
  • Genetic materials that are typically cryogenically preserved include sperm, oocytes, embryos and somatic cells. (wikipedia.org)
  • Women who have genetic abnormalities that may impair fertility are also more likely to desire to preserve their embryos. (icloudhospital.com)
  • Since its discovery, cryopreservation has contributed positive impacts on animal breeding as well as in genetic exchange, improvement, and conservation efforts. (tci-thaijo.org)
Development2
  • The assisted reproductive laboratories, also known as the andrology (the science of male reproduction, sperm biology and reproductive technology) and embryology (the branch of biology and medicine concerned with the study of embryos and their development) laboratories are critical components of a successful fertility program. (familyfertility.com)
  • Poor in vitro embryo development is a major obstacle in porcine assisted reproduction. (mdpi.com)
Human4
  • The best survival-developmental rates after cryopreservation of human ES cells. (ivf.net)
  • Colony-forming activity, as well as osteogenic and adipogenic capacities of primary human fetal liver (HFL) mesenchymal stem or progenitor cells (MSCs) were compared before and after cryopreservation using a standard three-step cooling protocol (Cryo3-S) or the same protocol with induced ice nucleation (Cryo3-IIN) and 5% and 10% w/v dimethyl sulphoxide (Me 2 SO). (cryoletters.org)
  • GM501 VitriStore Freeze/VitriStore Thaw are a set of ready-to-use media for vitrification and thawing of human embryos. (planer.com)
  • Rogulska O, Tykhvynska O, Revenko O, Grischuk V, Mazur S, Volkova N, Vasyliev N, Petrenko A, Petrenko Y. Novel cryopreservation approach providing off-the-shelf availability of human multipotent mesenchymal stromal cells for clinical applications. (in.ua)
Pregnancy1
  • Embryos randomized to the aCGH group implanted with greater efficiency, resulted in clinical pregnancy more often, and yielded a lower miscarriage rate than those selected without aCGH. (biomedcentral.com)
CPAs1
  • Vitrification, an alternative cryopreservation tool, has recently been shown to prevent ice formation and involves the use of a high concentration of CPAs and extremely high cooling rate. (molcells.org)
Mesenchymal1
  • Choice of vitrification mode for macroporous matrices seeded with mesenchymal stromal cells. (in.ua)
Cryotolerance1
  • Specific molecular mechanisms concerning sperm structures and functions were also examined relative to their association to cryotolerance, and spermiogram or seminogram modifications following cryopreservation procedures. (tci-thaijo.org)
Freeze5
  • The choice to freeze embryos is a personal one. (icloudhospital.com)
  • Other times, embryo cryopreservation allows women who are receiving medical procedures that may compromise fertility to freeze embryos and use them later. (icloudhospital.com)
  • Couples may choose to freeze additional embryos rather than kill them. (icloudhospital.com)
  • You must provide your permission to freeze embryos. (icloudhospital.com)
  • In a 4-well dish fill the first well with 300 μl of VitriStore Pre-vitrification Medium, the second with VitriStore Freeze Medium 1 and the third with VitriStore Freeze Medium 2. (planer.com)
Cryobiology1
  • Isolation and cryopreservation of placental cells: search for optimal biotechniques in experimental and regenerative medicine Problems of Cryobiology and Cryomedicine. (in.ua)