Embryo caenorhabditis elegans. Medical search. Frequent questions

To try to overcome some of these limitations in this study, we have used Caenorhabditis elegans (nematode) and Danio rerio embryos (zebrafish) as alternative assays for DART hazard assessment of some candidate chemicals being considered for a new commercial application. (nih.gov)
Eventually what it turned out is that a very famous nematode called Caenorhabditis elegans was the one that worked. (webofstories.com)
Specifically, they are using the early embryo of the soil nematode, Caenorhabditis elegans, as a model system because of the advantages it offers for the molecular analysis of mitosis. (pewtrusts.org)
Distribution of 212 kinase subfamilies throughout four kinomes: the yeast Saccharomyces cerevisiae , the nematode worm Caenorhabditis elegans , the fruit fly Drosophila melanogaster and human. (wormbook.org)
The invariant lineage of the nematode C. elegans [ 1 ] makes the organism a powerful model for studying developmental processes. (biomedcentral.com)
We are studying germ cell regulation primarily using the model organism C. elegans, a nematode, because of the large number of germ cells it contains, and because of the many experimental advantages it offers. (yale.edu)

The manual features primers on live imaging of a variety of standard model organisms including C. elegans, Drosophila, zebrafish, Xenopus, avian species, and mouse. (cshlpress.com)
Drosophila embryos are easily amenable to imaging because they are more transparent than the embryos of other model organisms, such as mice. (nature.com)
A confocal Z-stack from a transgenic Drosophila expressing the kinesin-5 motor KLP61F-GFP embryo during prometaphase phase 1. (ucsd.edu)
Dynamics of spindle microtubules and chromosomes during cycle 11 in a transgenic Drosophila embryo expressing GFP-tubulin (green) and histone-RFP (red). (ucsd.edu)
A confocal 4D-stack from Cycle 11 in a transgenic Drosophila embryo expressing gfp-tubulin (shown in green) and histone-rfp (shown in red). (ucsd.edu)
A confocal 4D-stack from a transgenic Drosophila embryo expressing lamin-GFP (green) and injected with rhodamine-conjugated tubulin (red) and anti-lamin-B antibodies during prometaphase. (ucsd.edu)

Our laboratory uses high-throughput functional genomics and computational approaches to study the cell biology, post-translational regulation, and evolution of molecular mechanisms during early embryonic development in the simple animal model C. elegans and related nematodes. (nyu.edu)
We discuss C. elegans embryonic development in case studies to illustrate how various HT approaches can be integrated and how biological insights can be gained from these approaches. (northwestern.edu)
The first C. elegans embryonic division of the P0 zygote is asymmetric and ge. (ucsd.edu)
1996. The SL1 trans-spliced leader RNA performs an essential embryonic function in Caenorhabditis elegans that can also be supplied by SL2 RNA. . (ucsb.edu)

The CeCDC-14 phosphatase is required for cytokinesis in the Caenorhabditis elegans embryo. (uchicago.edu)

The analyses were carried out in unfertilized oocytes, newly fertilized oocytes and embryos at the stages of mid-blastula transition and segmentation. (biomedcentral.com)
The two enrichment strategies allow probing of methyl-CpG protein interactions in early vertebrate oocytes and embryos. (biomedcentral.com)

Using QCANet, we were able to extract several quantitative criteria of embryogenesis from 11 early mouse embryos. (nature.com)
For example, since most diseases are multigenic in origin, we are generating genome-wide genetic interaction maps for genes with essential roles in the early embryo that will help us understand how these interactions affect embryogenesis. (nyu.edu)

As a graduate student at Cornell University, she employed the invertebrate model organism C. elegans, and made significant contributions to the discovery of RNA interference (RNAi) as well as understanding of asymmetric cell divisions. (ucsf.edu)
Pioneering work initiated in invertebrate model systems such as Caenorhabditis elegans has provided first drafts of catalogs of essential components, transcriptional regulatory diagrams and molecular interaction networks underlying developmental processes. (northwestern.edu)

Morphogenesis is important for creating the three germ layers of the early embryo (ectoderm, mesoderm, and endoderm) and for building up complex structures during organ development. (wikipedia.org)
The foxa gene is an integral component of the endoderm specification subcircuit of the endomesoderm gene regulatory network in the Strongylocentrotus purpuratus embryo. (biologists.com)
1997. end-1 encodes an apparent GATA factor that specifies the endoderm precursor in Caenorhabditis elegans embryos. . (ucsb.edu)

Here we combine the first large-scale serial electron tomography of whole mitotic spindles in early C. elegans embryos with live-cell imaging to reconstruct all microtubules in 3D and identify their plus- and minus-ends. (nature.com)

Zebrafish embryo stably expressing the microtubule-binding domain of ensconsin fused to three GFPs (EMTB-3GFP). (ucsd.edu)

Two null alleles of C. elegans ten-1 have previously been isolated using a reverse genetics approach. (biomedcentral.com)

The one-cell C. elegans embryo serves as a model for studying the establishment and maintenance of polarity. (biologists.com)
We have found that the C. elegans homolog of Lgl, LGL-1, has a role in polarity but is not essential. (biologists.com)
It localizes asymmetrically to the posterior of the early embryo in a PKC-3-dependent manner, and functions redundantly with PAR-2 to maintain polarity. (biologists.com)

A molecular explanation for this may be that several of the engulfment genes expressed by macrophages, including the ABC1 transporter (believed to be part of the phagocytic machinery conserved from Caenorhabditis elegans to mouse), are not upregulated by these 'stand-in' phagocytes. (bris.ac.uk)
Embryos homozygous for mutations in the unc-52, pat-2, pat-3, and unc- 112 genes of C. elegans exhibit a similar Pat phenotype. (illinois.edu)
Because the genes in C. elegans are related to those in higher organisms, the results from our studies should help us to understand how germ cells function in humans as well. (yale.edu)

In the Caenorhabditis elegans zygote, nuclear envelope breakdown (NEBD) of the parental pronuclei is spatially and temporally regulated during mitosis to promote the unification of the maternal and paternal genomes. (ijm.fr)

For a reverse experiment, DNA methylation in early Xenopus embryos was assessed by MBD affinity capture. (biomedcentral.com)

ten-1 is the C. elegans teneurin homolog with two transcripts, ten-1a and ten-1b , that respectively encode a long (TEN-1L) and short (TEN-1S) form of the protein. (biomedcentral.com)

Because maturing oocytes and early embryos lack appreciable transcription, posttranscriptional regulatory processes control their development. (elifesciences.org)
A landmark early example of using phylogenetics to describe cellular relationships was that of Sulston and colleagues reporting the development of C. elegans as deduced from meticulous visual observation [ 1 , 2 ]. (biorxiv.org)
In early embryos, cells are loosely connected to each other. (nature.com)
For time-lapse observation of early-stage D rosophila embryos, Keller et al. (nature.com)
In the early embryo, a myosin II-dependent contraction of the cortical meshwork asymmetrically distributes the highly conserved PDZ proteins PAR-3 and PAR-6, as well as an atypical protein kinase C (PKC-3), to the anterior. (biologists.com)
Here, by using genome editing and depletion of tubulin isotypes following RNAi, we demonstrate that four tubulin isotypes (hereafter referred to as α1, α2, β1 and β2) cooperatively confer distinct MT properties in Caenorhabditis elegans early embryos. (elsevierpure.com)
This movie illustrates the early division up to the four-cell stage of a C. elegans embryo depleted of npl-4 through RNAi. (ucsd.edu)
This time-lapse series illustrates the early division up to the four-cell stage of a C. elegans embryo depleted of npl-4 through RNAi. (ucsd.edu)

To analyse the time-series 3D microscopic images of developing embryos with fluorescently labelled nuclei, these studies used image segmentation. (nature.com)
AceTree, a software application first released in 2006, facilitates exploration, curation and editing of tracked C. elegans nuclei in 4-dimensional (4D) fluorescence microscopy datasets. (biomedcentral.com)

Anterior and posterior regions of a C. elegans embryo are determined by PAR protein distribution. (cellimagelibrary.org)
In C. elegans , the ten-1 gene can be transcribed from two distinct promoters to produce the transcripts ten-1a and ten-1b , which respectively encode two isoforms of the protein: TEN-1L, which contains all the teneurin domains described above (see Fig. 1F ), and TEN-1S, which lacks the ICD but contains the rest of the protein including the transmembrane domain. (biomedcentral.com)
The zinc finger protein DIE-1 is required for late events during epithelial cell rearrangement in C. elegans. (ucsb.edu)

Recording and contextualizing the science of embryos, development, and reproduction. (asu.edu)
His research interests are in the mechanisms involved in the development of the embryo. (webofstories.com)
The endomesoderm gene regulatory network (GRN) models the transcriptional control system defining vegetal specification of the sea urchin( Strongylocentrotus purpuratus ) embryo during the first 30 hours of development. (biologists.com)
Thus, embryo development is highly dynamic. (nature.com)
Today, it is generally thought that the combination of determinants deposited by the mother during oogenesis and the inductive signals between different cells trigger the specification of different cell lineages during development of the embryo [ 7 , 8 ]. (biomedcentral.com)
Caenorhabditis elegans), and some deuterostomes (e.g., echinoderms and ascidians) exhibit determinative development. (iospress.com)
The embryos of larger arthropods and deuterostomes with well-provisioned eggs or viviparity, on the other hand, exhibit regulative development, while their larval "set-aside" or adult stem cells function in the growth, maintenance, and regulation of organ size coupled to constrained proliferation and cell turnover. (iospress.com)

Developmental dynamics of gene expression and alternative polyadenylation in the Caenorhabditis elegans germline. (nyu.edu)
Transcriptomic analyses provide an efficient approach to explore the temporal gene expression profiles in embryos and to search for the developmental regulators. (biomedcentral.com)
We address these questions in vivo with unprecedented cell specificity and comprehensiveness, utilizing innovative methods to investigate how genome structure and organization influences gene expression specifically in the C. elegans germ line. (yale.edu)

In this paper, we set out to identify the cytoskeletal ultrastructure in C. elegans mitotic spindles that underlies this function, and how this ultrastructure is generated, using a combination of large-scale electron tomography, light microscopy and mathematical modelling. (nature.com)

Immunohistology note: germ granules (P granules) and muscle cells in fixed C. elegans embryos (recognized two antigens). (uiowa.edu)

We have previously established that the spontaneous, genome-wide rate of gene duplication in C. elegans is two orders of magnitude higher than the point mutation rate [ 8 ]. (biomedcentral.com)

Transcripts found in unfertilized oocytes also encoded a large number of proteins implicated in cell adherence, tight junction and focal adhesion, suggesting high complexity in terms of structure and cellular interactions in embryos prior to midblastula transition (MBT). (biomedcentral.com)

Where researchers once struggled to connect events at static timepoints, imaging tools now offer the ability to visualize the dynamic form and function of molecules, cells, tissues, and whole embryos throughout the entire developmental process. (cshlpress.com)
Further techniques are organized by the level of visualization they provide, from cells to tissues and organs to whole embryos. (cshlpress.com)
Patterns are ubiquitous in living systems and underlie the dynamic organization of cells, tissues, and embryos. (mpi-cbg.de)

The authors propose that the asymmetric segregation of the NuRD complex in C. elegans is regulated in a V-ATPase-dependent manner, that this plays a crucial role in determining the differential expression of the apoptosis activator egl-1 and that it is therefore critical for the life/death fate decision in this species. (elifesciences.org)
We showed that QCANet can be applied not only to developing mouse embryos but also to developing embryos of two other model species. (nature.com)
Specificity note: Caenorhabditis elegans (species cross-reaction is not tested). (uiowa.edu)
Notably, although the phosphosites are not conserved between human and C. elegans nucleoporins, they are located in IDRs in both species. (ijm.fr)

Here, we demonstrate that the nucleosome remodeling and deacetylase (NuRD) complex is asymmetrically segregated into the surviving daughter cell rather than the apoptotic one during ACDs in Caenorhabditis elegans . (elifesciences.org)

For example, in the neural plate of the embryo this system operates to generate a population of neuronal precursor cells in which NeuroD is highly expressed. (wikipedia.org)
Because the cells in embryos are considerably crowded, an algorithm to segment individual cells in detail and accurately is needed. (nature.com)
At the 8-cell stage, the embryo becomes compact, and the cells form a spherical mass called a morula. (nature.com)
Biologists use GFP to study cells in embryos and fetuses during developmental processes. (asu.edu)
In the wild-type embryo, these dead cells are rapidly engulfed and cleared by macrophages. (bris.ac.uk)
None of the body neurons examined show any defects in the ten-1(ok641) mutant, but genetic interaction studies reveal that ten-1(ok641) is synthetic lethal with sax-3, unc-34 and unc-73 , and examination of the hypodermal cells in embryos of the ten-1(ok641) mutant point to a role of ten-1 during hypodermal cell morphogenesis. (biomedcentral.com)

Asymmetric segregation of NuRD during ACDs of C. elegans Q neuroblast. (elifesciences.org)

PAR-2, shown in green marks the posterior region of the embryo and PAR-6, shown in red, marks the ante. (cellimagelibrary.org)

Here we use Caenorhabditis elegans to study the role of centrosomes in setting spindle length. (princeton.edu)

In terms of the pattern of structures, this is controlled by a re-activation of signals active in the embryo. (wikipedia.org)
Efficient production of monoclonal antibodies recognizing specific structures in Caenorhabditis elegans embryos using an antigen subtraction method. (uiowa.edu)

We investigated whether CNVs constitute a common mechanism of genetic change during adaptation in experimental Caenorhabditis elegans populations. (biomedcentral.com)

Indeed, that such forces exist in C. elegans is evidenced by the maintenance of half-spindle lengths throughout mitosis 5 and in many perturbations experiments. (nature.com)

Recently, the three-dimensional time-lapse live imaging and delineated developmental programs in the roundworm Caenorhabditis elegans provide an excellent platform for establishing quantitative models. (aps.org)

This chapter will introduce the diversity of kinases in C. elegans , and compare them to those of fungi and other metazoans, as well as to preliminary results from analysis of the C. briggsae kinome. (wormbook.org)

It is one aim of our study to identify the nature of the KMT-centrosome connection in C. elegans , which is so far unknown. (nature.com)
In this paper, we study two popular computational models for multicellular systems, i.e., the phase-field model and the coarse-grained model, and compare their performance in characterizing the cell morphologies, cell adhesion, and cell stiffness in a real C. elegans embryo. (aps.org)

We show that in a macrophageless mouse embryo, null for the haemopoetic-lineage-specific transcription factor, PU.1, the task of phagocytosis is taken over by 'stand-in' mesenchymal neighbours in a clear example of cell redundancy. (bris.ac.uk)

Caenorhabditis elegans represents a valuable model for investigating ACD, given its invariant cell lineage and conserved mechanisms of ACD. (elifesciences.org)
Apoptosis is one of the key tools used by an embryo to regulate cell numbers and sculpt body shape. (bris.ac.uk)
Revisions to canonical name assignment allow for the free orientation of embryos in 3-dimensional space and an entirely new 3-dimensional viewing window provides a new suite of methods for exploring cell positions. (biomedcentral.com)
2023. Sperm-contributed centrioles segregate stochastically into blastomeres of 4-cell stage Caenorhabditis elegans embryos . (cabimer.es)

This generally involves the action of cytoplasmic determinants, located within parts of the fertilized egg, and of inductive signals emitted from signaling centers in the embryo. (wikipedia.org)

Now I'd got this culture, C. elegans , it had been isolated in Bristol and a strain had also been isolated in France where a man called Nigon had worked on it, that was called the Bergerac strain. (webofstories.com)

Anatomy31

Organisms13

Diseases2

Chemicals and Drugs34

Analytical, Diagnostic and Therapeutic Techniques and Equipment14

Psychiatry and Psychology3

Phenomena and Processes75

Information Science4

Health Care1

Nematode6

Drosophila6

Embryonic4

Cytokinesis1

Oocytes and embryos2

Embryogenesis2

Invertebrate model2

Endoderm3

Microtubules1

Zebrafish1

Genetics1

Polarity3

Genes3

Zygote1

Xenopus1

Homolog1

Early8

Nuclei2

Protein3

Development7

Gene expression3

Mitotic1

Germ1

Genome1

Transcripts1

Tissues3

Species4

Apoptotic1

Cells6

Asymmetric1

Posterior1

Centrosomes1

Structures2

Experimental1

Spindle1

Quantitative1

Diversity1

Study2

Transcription1

Cell4

Signals1

Strain1