AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesDisciplines and OccupationsTechnology, Industry, AgricultureInformation ScienceHealth Care
Chromatography, High Pressure LiquidChromatography, AffinityChromatography, GelChromatographyChromatography, Ion ExchangeChromatography, GasChromatography, LiquidChromatography, Thin LayerMolecular WeightChromatography, DEAE-CelluloseElectrophoresis, Polyacrylamide GelChromatography, AgaroseMass SpectrometryChromatography, Reverse-PhaseKineticsSepharoseMolecular Sequence DataAmino AcidsAmino Acid SequenceGas Chromatography-Mass SpectrometryHydrogen-Ion ConcentrationChemical FractionationSpectrophotometry, UltravioletChromatography, PaperCattleChemistryChemical PhenomenaTandem Mass SpectrometrySubstrate SpecificityMethodsSpectrometry, Mass, Electrospray IonizationIsoelectric FocusingCountercurrent DistributionCarbohydratesAdsorptionDrug StabilityIsoelectric PointRabbitsAcetonitrilesTrypsinEscherichia coliIndicators and ReagentsLiverCalibrationPeptide FragmentsHydroxyapatitesSolid Phase ExtractionSolubilityReference StandardsProtein BindingLimit of DetectionMacromolecular SubstancesImmunodiffusionMicrochemistryAnion Exchange ResinsCarbohydrate SequencePeptidesReproducibility of ResultsTemperatureOligosaccharidesRadioimmunoassayHydrolysisChromatography, Micellar Electrokinetic CapillaryAmmonium SulfateGlycoproteinsChemical PrecipitationRecombinant ProteinsMagnetic Resonance SpectroscopyHot TemperatureStereoisomerismCentrifugation, Density GradientPolysaccharidesIsoenzymesDNABinding SitesCell LineUltracentrifugationBase SequenceSolventsBiological AssayMethanolTrifluoroacetic AcidRadionuclide GeneratorsSwineLectinsSensitivity and SpecificityElectrophoresis, DiscChemistry Techniques, AnalyticalElectrophoresisBrain ChemistrySpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationCloning, MolecularRats, Inbred StrainsMolecular StructureBuffersImmunoelectrophoresisGlycoside HydrolasesSpecies SpecificityErythrocytesIsomerism
High performance liSeparationAnion exchange chromatographyHPLCSize exclusion chroLiquid Chromatography-Tandem Mass SpectroPurificationPerformance supercritical fluid chromatoGradientReverse-phase chromatoPhase liquid chromatographyExchange chromatographySupercriticalSolventStationaryRetentionProteinPermeationCompoundsAmino acidsHexaneAffinityHILICDetectionAntibodyResinsMonolithicPeaksRobustColumnPrecipitationEluentOrganicAdvancesCarrier gasColumnsOppositeProcessesExperimentTypicallyTechniquesCompoundTimeProcedureTechniqueFigure
High performance li5
  • A theoretical description of analyte retention in high-performance liquid chromatography (HPLC) has been the subject of various publications. (springer.com)
  • Hydrophilic interaction liquid chromatography (HILIC) is an alternative high-performance liquid chromatography (HPLC) mode for separating polar compounds. (springer.com)
  • The five sweeteners are analysed by high performance liquid chromatography through separation on a C18 reverse-phase column coupled with a diode array detector and charged aerosol detector connected in series (HPLC/UV-CAD). (oiv.int)
  • Selective separation of the analytes is achieved using high-performance liquid chromatography with a gradient elution program. (cdc.gov)
  • 1 extraction was carried out using immunoaffinity column, separated by reversed-phase (C-18) high performance liquid chromatography (HPLC) , and quantified by fluorescence detector. (bvsalud.org)
Separation19
  • This article will include data obtained from method development, optimization and analysis of multiple vitamin D metabolites on an ultra performance supercritical fluid chromatography tandem-mass spectrometry (UPSFC-MS/MS). This includes chromatograms from column screening to confirm the most suitable column for analyte separation. (sun.ac.za)
  • Chromatogram data from UHPLC vitamin D analysis is also presented in order to compare the separation and elution of vitamin D metabolites using UPSFC and UHPLC. (sun.ac.za)
  • Chromatography in general is a separation method in which the partition equilibrium of a compound between two phases is permanently disturbed since one of the phases is changing/exchanged (mobile phase). (stackexchange.com)
  • Ladislav Sojak published a freely available 35-page review Separation and identification of isomeric hydrocarbons by capillary gas chromatography and hyphenated spectrometric techniques in Petroleum & Coal , 2004 , 4(3) , 1-35 ( PDF ). (stackexchange.com)
  • The "elution time" of a solute is the time between the start of the separation (the time at which the solute enters the column) and the time at which the solute elutes. (wikipedia.org)
  • NP-LC has been widely used to separate various compounds, from nonpolar to highly polar compounds (note that chromatography was first introduced as a method used in separation science). (springer.com)
  • For historical reasons, it has been reported that HILIC is a variant of normal phase liquid chromatography, but the separation mechanism used in HILIC is more complicated than that in NP-LC. (springer.com)
  • Figure 2 shows how HILIC complements other areas of chromatography and extends the range of separation options. (springer.com)
  • Hydrophilic interaction liquid chromatography has established itself as the separation mode of choice for uncharged highly hydrophilic and amphiphilic compounds that are too polar to be well retained in RP-LC but have insufficient charge to allow effective electrostatic retention in ion-exchange chromatography. (springer.com)
  • Join us on a journey through the monolith matrix to discover how convective chromatography supports the robust separation of large biomolecules. (sartorius.com)
  • The involved chemistry relies on basic knowledge of element separation involving radiochemical techniques such as liquid-liquid extraction, ion chromatography, pyrochemistry or precipitation. (psi.ch)
  • The gradual elution of lanthanides for the separation of 171 Tm. (psi.ch)
  • Analytical-scale reverse-phase chromatography of these Schiff's bases produced six peaks, indicating separation of the enantiomeric forms of the three gossypol compounds. (usda.gov)
  • Ionic and ionizable compounds typically rely on separation by ion exchange chromatography (IC) or ion pairing reversed-phase chromatography (IP-RPLC). (news-medical.net)
  • However, the separation of oligonucleotides by size exclusion chromatography (SEC) is a valuable alternative. (chromatographytoday.com)
  • Previously, we have developed a gradient elution system for pillar array columns, which achieved faster separation than isocratic elution. (go.jp)
  • Wang has developed new chromatography separation techniques that could separate rare earth elements first from other impurities and then from each other by using only a few chromatography units. (phys.org)
  • GC/MS analysis allows separation of compounds based on their retention time within a chromatography column and identification of each compound by the characteristic fragments into which a given chemical is broken following ionization of the compound. (medscape.com)
  • The high separation power and short analysis time obtained demonstrate for the first time that supercritical fluid chromatography is a fast and reliable technique for the analysis of lignin-derived phenols in complex environmental samples. (lu.se)
Anion exchange chromatography4
  • Ion pair reversed phase liquid chromatography (IP-RP) and anion exchange chromatography (AEX) are the most used methods for oligonucleotides analysis. (chromatographytoday.com)
  • This article shows how anion-exchange chromatography can fulfill that need. (bioprocessintl.com)
  • The monitoring system used, both in the on- and off-line mode, was based on continuous flow microdialysis sampling (CFMS) coupled to anion exchange chromatography and integrated pulsed electrochemical detection (IPED). (lu.se)
  • A microdialysis probe equipped with a 5-mm polysulfone (SPS 4005) membrane, with a molecular-weight cut-off of 5 kDa, was used to sample the hydrolysis products of native wheat starch at 90 degrees C. Characteristic fingerprint separations were achieved by anion exchange chromatography after enzymatic hydrolysis. (lu.se)
HPLC2
  • Predict the order of elution for a mixture containing caffeine and benzoic acid, using a reverse phase HPLC. (stackexchange.com)
  • Since for HPLC, the mobile phase is the polar phase, elution of polar compounds will be favoured hence benzoic acid will be eluted first. (stackexchange.com)
Size exclusion chro4
Liquid Chromatography-Tandem Mass Spectro1
  • Therefore, in the present study, we performed untargeted lipid profiling of abalone tissues at different developmental stages as well as the hemolymph of mature female and male abalones using ultrahigh-performance liquid chromatography-tandem mass spectrometry. (hindawi.com)
Purification4
  • Sartorius experts represent extensive experience in chromatography and purification of biomolecules as well as analytical methods development. (sartorius.com)
  • Benefit from all the attributes of monolithic chromatography with a range of strong and weak anion and cation exchangers to meet the needs for purification of large biomolecules. (sartorius.com)
  • Affinity chromatography provides an excellent platform for protein purification, which is a key step in the large scale downstream processing of therapeutic monoclonal antibodies (Mabs). (nih.gov)
  • Protein A chromatography constitutes the gold standard for Mab purification. (nih.gov)
Performance supercritical fluid chromato3
  • The data presented is related to the research article "Analysis of multiple vitamin D metabolites by ultra performance supercritical fluid chromatography-tandem mass spectrometry (UPSFC-MS/MS)" (Jenkinson et al. (sun.ac.za)
  • Alkaline cupric oxide (CuO) oxidation is one of the mostly performance supercritical fluid chromatography (UHPSFC) adopted methods for analyzing lignin in various complex using columns with sub-2 m packing can further improve environmental matrices such as soils and sediments [9]. (lu.se)
  • UHPSFC , ultra- pacted by other parameters, such as the addition of the mod- high performance supercritical fluid chromatography ifier and the change of the mobile phase density [20]. (lu.se)
Gradient7
  • In contrast to RP-LC, gradient elution HILIC begins with a low-polarity organic solvent and elutes polar analytes by increasing the polar aqueous content [ 13 ]. (springer.com)
  • The model and model parameters were verified from 10 to 50% MeCN, loading up to 2 CV, and linear velocities up to 1.69 cm/min, in both linear gradient elution and stepwise elution. (aiche.org)
  • It has the advantage of 3% higher yield, at least four times the productivity, 2.5 times the product concentration and 1/3 the protein residence time compared to linear gradient elution. (aiche.org)
  • In this study, we validated gradient elution in microchip liquid chromatography (LC) and investigated the retention and bandwidth predictions of fluorescently labeled aliphatic amines in fast gradient elution chromatography using semi-empirical retention models. (go.jp)
  • The retention times and peak widths under different gradient elution programs were predicted by three solvent strength models and compared with the experimental results. (go.jp)
  • The results showed that the solvent strength models could be utilized for predicting the retention times and peak widths under gradient elution in the microchip LC system and that the gradient elution program for pillar array columns worked efficiently. (go.jp)
  • The mobile phase, typically a gradient of water and organic solvent, facilitates the elution of amino acids from the column. (pharmiweb.com)
Reverse-phase chromato1
  • This contrasts with the order seen in standard reverse-phase chromatography, where elution occurs in the order of least to most hydrophilic. (news-medical.net)
Phase liquid chromatography3
Exchange chromatography2
Supercritical2
Solvent3
  • Elution then is the process of removing analytes from the adsorbent by running a solvent, called an "eluent", past the adsorbent/analyte complex. (wikipedia.org)
  • As the solvent molecules "elute", or travel down through the chromatography column, they can either pass by the adsorbent/analyte complex or they can displace the analyte by binding to the adsorbent in its place. (wikipedia.org)
  • What were your column chromatography solvent(s)? (chemicalforums.com)
Stationary6
  • Hydrophilic interaction liquid chromatography (HILIC) provides an alternative approach to effectively separate small polar compounds on polar stationary phases. (springer.com)
  • Ion chromatography relies on distribution ratios between a solid stationary and a liquid mobile phase. (psi.ch)
  • Hydrophilic interaction chromatography (HILIC) is a liquid chromatography technique that is used to separate moderate to highly hydrophilic and polar compounds based on their retention on a hydrophilic stationary phase versus the hydrophilic aqueous polar organic mobile phase. (news-medical.net)
  • Secondary forces, such as dipole-dipole, electrostatic, and hydrogen bonding interactions influence this partition, resulting in retention of the compound on the stationary phase or elution in the mobile phase. (news-medical.net)
  • Although dsDNA has the same length as its single-stranded counterpart, the dsDNA elutes at lower elution volumes when separated by SEC. This behaviour is most probably due to the larger hydrodynamic radius of dsDNA compared to ssDNA, which results in faster diffusion through the stationary phase. (chromatographytoday.com)
  • I understand that the purpose of the column chromatography is to separate away any unreacted benzoin (the hydroxyl group would be attracted to the stationary silica layer). (chemicalforums.com)
Retention1
  • Enhancing our understanding of retention behavior in HILIC increases the scope of possible applications of liquid chromatography. (springer.com)
Protein3
  • The ability of protein A affinity chromatography to achieve 95% purity in one simple step was the runway that got recombinant immunotherapy off the ground and made it available to millions. (bioprocessintl.com)
  • Furthermore, gel column chromatography on Sephadex G-150 indicated that the elution curves of the carbohydrate and protein overlapped significantly in sample P ( Figure 1 ). (scirp.org)
  • Gas chromatography is suitable for the analysis of complex protein samples. (pharmiweb.com)
Permeation3
  • Sample preparation steps for adipose tissue vary but, in general, involve a lipid extraction step followed by a clean-up procedure involving gel permeation chromatography (GPC) and/or Florisil column clean-up. (cdc.gov)
  • The filtrate was cleaned up by gel permeation chromatography utilizing elution with hexane and ethyl-acetate. (cdc.gov)
  • The glycopeptide fractions were further purified by gel-permeation chromatography and two purified glycopeptides, D2H, D2L, were obtained. (scirp.org)
Compounds6
  • Such series are useful for determining necessary solvents needed for chromatography of chemical compounds. (wikipedia.org)
  • For example, it is suitable for analyzing compounds in complex systems that always elute near the void in reserved-phase chromatography. (springer.com)
  • DuPont chromatography resins quickly and efficiently elute the salts and non-anion compounds while retaining the anionic compounds. (dupont.com)
  • Over the years, technologic advances in electronics and detectors have allowed GC/MS to detect ever smaller concentrations of compounds, as has the addition of other analytical techniques, such as liquid chromatography. (medscape.com)
  • The successful chromatography of Tris-BP on the short, thin-film column with both inlet systems illustrates the effect of film thickness and column length on thermally labile compounds. (epa.gov)
  • The new ultra-high performance super- critical fluid chromatography method was also applied for the qualitative and quantitative analysis of lignin-derived phenolic compounds obtained upon alkaline cupric oxide oxida- tion of a commercial humic acid. (lu.se)
Amino acids3
Hexane3
  • The preparation step used for measuring heptachlor epoxide in blood and serum involves lipid extraction, clean-up with column chromatography, and elution with acetonitrile, hexane, and methylene chloride (Burse et al. (cdc.gov)
  • elution with acetonitrile, hexane and methylene chloride. (cdc.gov)
  • The diastereomers derived from some aliphatic amines were resolved by a reversed-phase chromatography with water-acetonitrile or normal-phase chromatography with n-hexane-ethyl acetate as the eluent. (unboundmedicine.com)
Affinity1
  • However, the required acidic conditions (2.8-3.5) for elution from the affinity matrix limit their applicability, particularly for next generation antibodies and antibody fusion proteins, since denaturation and irreversible aggregation can occur due to the acidic buffer conditions. (nih.gov)
HILIC1
Detection1
  • The common method used is gas chromatography (GC) coupled with electron capture detection (ECD) followed by identification using GC/mass spectrometry (MS). Since evidence indicates that heptachlor is metabolized to heptachlor epoxide in mammals, exposure to heptachlor is usually measured by determining levels of heptachlor epoxide in biological media. (cdc.gov)
Antibody1
  • Antibody elution is the process of removing antibodies that are attached to their targets, such as the surface of red blood cells. (wikipedia.org)
Resins1
  • In organic acid fermentation, DuPont's acrylic chromatography resins can separate the organic acids from contaminating salts and residual dextrose with sharp and efficient elution peaks. (dupont.com)
Monolithic1
Peaks1
  • The elution order of the peaks was found to vary with the polarity of the mobile phase. (usda.gov)
Robust1
  • The processes involve ligand-assisted elution or displacement chromatography methods using robust, low-cost, inorganic sorbent titania or polymeric sorbents. (phys.org)
Column7
  • In a liquid chromatography experiment, for example, an analyte is generally adsorbed ("bound to") an adsorbent in a liquid chromatography column. (wikipedia.org)
  • Predicting and controlling the order of elution is a key aspect of column chromatographic methods. (wikipedia.org)
  • The sample was then analyzed by gas chromatography utilizing a DB-1 column. (cdc.gov)
  • After reacting and isolating the crude product, we did recrystallization, and afterwards, column chromatography. (chemicalforums.com)
  • If we did the column chromatography first, would water be instead attracted to the silica layer? (chemicalforums.com)
  • The conditions were successful in that elution times and column operating temperatures could be decreased to such an extent that the thermal lability of the compound was not prohibitive. (epa.gov)
  • in gas chromatography , the conditions for adjustment of the column dimensions, injection volume, split ratio, injection port and transfer-line temperatures (the latter two being new for Ph. (edqm.eu)
Precipitation1
  • To screen and characterize the analgesic components from Marasmius androsaceus , the water-soluble extracts from the mycelium of M. androsaceus were isolated by ethanol precipitation followed by macroporous resin chromatography and activity-based fractionation. (scirp.org)
Eluent1
  • In the same way, the elution volume is the volume of eluent required to cause elution. (wikipedia.org)
Organic1
  • My other interests include organic modification of surfaces as it pertains to dye-sensitized solar cells, chromatography and drug elution. (sdstate.edu)
Advances1
Carrier gas2
Columns1
  • Figure 3: Comparison of the elution volume of ssDNA and dsDNA by using YMC-Pack Diol columns with different pore sizes. (chromatographytoday.com)
Opposite1
  • The opposite elution order was obtained with the use of R(+)-DBD-Pro-COCl, instead of S(-)-DBD-Pro-COCl. (unboundmedicine.com)
Processes1
  • A model-based approach is used to develop reversed-phase chromatography (RPC) processes for purifying lispro human proinsulin (lispro-HPI) from a complex multi-component mixture with ~70% impurities and unknown components. (aiche.org)
Experiment1
Typically1
  • If the screening test is positive, then that positive result must be confirmed, typically with gas chromatography/mass spectrometry (GC/MS). (medscape.com)
Techniques1
Compound1
Time1
  • The di- and tri- carboxylic acids will be more strongly retained and will have a longer elution time than the mono-carboxylic acids. (dupont.com)
Procedure1
  • Here we describe a generic procedure for the generation of antigen-specific chromatography ligands with tailor-made elution conditions. (nih.gov)
Technique1
  • Under standard conditions for a known mix of solutes in a certain technique, the elution volume may be enough information to identify solutes. (wikipedia.org)
Figure1
  • Next, the component sugars of sample P were investigated by gas chromatography, and the results are shown in Figure 2 . (scirp.org)