AnatomyOrganismsChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesInformation ScienceHealth Care
Substrate SpecificityEnzyme-Linked Immunosorbent AssayKineticsMolecular Sequence DataAmino Acid SequenceBinding SitesCatalysisModels, MolecularRecombinant ProteinsProtein BindingEscherichia coliHydrolysisCatalytic DomainHydrogen-Ion ConcentrationCrystallography, X-RayBase SequenceProtein ConformationProtein Structure, TertiaryPhosphorylationSequence Homology, Amino AcidMutagenesis, Site-DirectedMutationStructure-Activity RelationshipOxidation-ReductionBacterial ProteinsCattleCloning, MolecularCell LineElectrophoresis, Polyacrylamide GelSensitivity and SpecificityMolecular StructureEnzyme ActivationAdenosine TriphosphateMolecular WeightModels, ChemicalPeptidesImmunoglobulin GCells, CulturedSequence AlignmentChromatography, High Pressure LiquidStereoisomerismOligopeptidesIsoenzymesEscherichia coli ProteinsEndopeptidasesSubstrate CyclingTemperatureBlotting, WesternTime FactorsLiverAmino AcidsEnzyme InhibitorsModels, BiologicalEnzyme StabilitySaccharomyces cerevisiaeBiological TransportRecombinant Fusion ProteinsAmino Acid SubstitutionCarrier ProteinsRabbitsSerine EndopeptidasesAntibodies, BacterialDNA PrimersGlucoseSwineNADProtein Structure, SecondaryMembrane ProteinsImmunoblottingPeptide FragmentsOxidoreductasesDNAChromatography, GelMass SpectrometryPhosphoproteinsMixed Function OxygenasesAmino Acid MotifsSerologic TestsSignal TransductionAntibody SpecificityProtein KinasesTyrosineImmunoglobulin MProteinsAntibodies, ViralPlasmidsArginineCytochrome P-450 Enzyme SystemAntibodies, MonoclonalAdenosine TriphosphatasesBinding, CompetitiveRNA, MessengerCross ReactionsMagnetic Resonance SpectroscopyPeptide HydrolasesCell MembraneLysineMembrane Transport ProteinsCysteine EndopeptidasesProtein Folding
BufferConjugateChemiluminescent SubstrateAlkaline PhosphataseQuantitativeAntigenPrimary AntibodiesSensitivitySandwichReactivitySolutionProteinMicroplateChromatographyQuantitationReagentsSupernatantsConventionalAssay ProcedureQualitativePlateKitsECISProductsSerum and plasmaCommercially availableIncubationReactsEnzyme-linkedEnzymaticLaboratoriesAssaysTestReproducibilityImmunohistochemistryComponentDiagnosticAntibodies directed
Buffer2
  • The system includes AttoPhos® Substrate, AttoPhos® Buffer and Calibration Solution. (selectscience.net)
  • ELISA Max™ Set Deluxe includes pre-titrated capture antibody, pre-titrated biotinylated detection antibody, pre-titrated Avidin-HRP, conveniently-sized assay calibration standards, easy-to-follow instructions, as well as, assay diluent, coating buffer, and TMB substrate. (biolegend.com)
Conjugate2
  • Excess enzyme conjugate is washed off and TMB Chromogenic substrate is added. (rapidtest.com)
  • The wells are washed to remove unreacted conjugate, 3) the microwells containing immobilized peroxidase conjugate are incubated with peroxidase substrate solution. (cdc.gov)
Chemiluminescent Substrate2
  • SuperSignal ELISA Pico Chemiluminescent Substrate is optimized to generate an intense light signal and provide exceptional performance in luminometer-based assays. (selectscience.net)
  • Abbkine SuperKine™ West Pico PLUS Chemiluminescent Substrate provides a highly sensitive color developing solution to enhance the chemical signal of immunological experiments. (gaiascience.com.sg)
Alkaline Phosphatase6
  • The AttoPhos® AP Fluorescent Substrate System provides a highly sensitive fluorescent alkaline phosphatase substrate. (selectscience.net)
  • Alkaline Elisa Laboratories manufactures the substrate solution concentration for alkaline phosphatase elisa reagents distributed by Genprice. (muzeumamonitow.pl)
  • The Substrate Solution Concentration For Alkaline Phosphatase Elisa reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. (muzeumamonitow.pl)
  • Description: Quantitative sandwich ELISA for measuring Goat Alkaline phosphatase (ALP) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. (muzeumamonitow.pl)
  • Description: A sandwich ELISA kit for detection of Alkaline Phosphatase from Rat in samples from blood, serum, plasma, cell culture fluid and other biological fluids. (muzeumamonitow.pl)
  • Description: Quantitative sandwich ELISA for measuring Fish Alkaline phosphatase (ALP) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. (muzeumamonitow.pl)
Quantitative6
  • Abcam's Urokinase type Plasminogen Activator Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Urokinase type Plasminogen Activator (uPA) in plasma, serum, urine, milk, cell culture supernatants and tissue extracts. (abcam.com)
  • This ELISA kit is used for quantitative protein detection, establishing normal range. (biovision.com)
  • The Human CD97 Enzyme-Linked Immunosorbent Assay (ELISA) Kit (ab213763) is designed for the quantitative measurement of Human CD97 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). (abcam.com)
  • AgraQuant ® M1 test kits are accurate and reliable enzyme-linked immunosorbent assays (ELISA) in a quantitative format. (romerlabs.com)
  • The Diagnostic Automation RSV IgG Antibody ELISA Test Kit has been designed for the detection and the quantitative determination of specific IgG antibodies against Respiratory syncytial virus in human serum and plasma. (rapidtest.com)
  • This ELISA kit applies to the in vitro quantitative determination of Rabbit MMP-13 concentrations in Serum, plasma and other biological fluids. (caslab.com)
Antigen5
  • Performing an ELISA involves at least one antibody with specificity for a particular antigen. (wikipedia.org)
  • The sample with an unknown amount of antigen is immobilized on a solid support (usually a polystyrene microtiter plate) either non-specifically (via adsorption to the surface) or specifically (via capture by another antibody specific to the same antigen, in a "sandwich" ELISA). (wikipedia.org)
  • After the final wash step, the plate is developed by adding an enzymatic substrate to produce a visible signal, which indicates the quantity of antigen in the sample. (wikipedia.org)
  • females collected were tested for the presence of two phenotypes of P. vivax (PVK210 and PVK247) antigen by enzyme-linked immunosorbent assay (ELISA). (who.int)
  • To get a cost-effective, high throughput, ELISA for estimation of human tissue kallikreins we have now developed an indirect, back-titration style, Time Resolved ImmunoFluorometric (TRIF) ELISA that uses only 1 antigen-specific moAB and a general polyclonal antibody. (bvsalud.org)
Primary Antibodies1
  • Sensitivity and cost per test - A sensitive substrate could allow you to achieve a better dilution on costly primary antibodies. (2bscientific.com)
Sensitivity4
  • Download the full guide for full specifications of substrates, sensitivity and combinations for multiple labelling. (2bscientific.com)
  • A large offering of ELISA substrates, starting with our Uptima TMB solutions (HRP substrate) with superior sensitivity and stability, at a nice price! (interchim.com)
  • The Bayesian posterior estimate for sensitivity was 89.6 (95% PI: 79.9-95.8), 96.8 (95% PI: 92.3-99.1), and 94 (95% PI: 87.8-97.5) and for specificity was 84.5 (95% PI: 68-94.98), 96.3 (95% PI: 91.7-98.8), and 88.5 (95% PI: 81-93.8) for RBT, I-ELISA, and CFT, respectively. (hindawi.com)
  • In this study I-ELISA was found with the best sensitivity and specificity estimates 96.8 (95% PI: 92.3-99.1) and 96.3 (95% PI: 91.7-98.8), compared to both CFT and RBPT. (hindawi.com)
Sandwich9
  • This DuoSet IC ELISA contains the basic components required for the development of sandwich ELISAs to measure total HSP27 in cell lysates. (rndsystems.com)
  • This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human IL-19. (rndsystems.com)
  • Treatment of Hep G2 cells with H 2 O 2 stimulates phosphorylation of ACC at Ser79, detected by the PathScan ® Phospho-ACC (Ser79) Sandwich ELISA Kit #7986, but does not affect the levels of total ACC detected by PathScan ® Total ACC Sandwich ELISA Kit #7996. (cellsignal.com)
  • The relationship between the protein concentration of lysates from untreated and H 2 O 2 -treated Hep G2 cells and the absorbance at 450 nm using the PathScan ® Phospho-Acetyl-CoA Carboxylase (Ser79) Sandwich ELISA Kit is shown. (cellsignal.com)
  • The PathScan ® Phospho-Acetyl-CoA Carboxylase (Ser79) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of acetyl-CoA carboxylase (ACC) when phosphorylated at Ser79. (cellsignal.com)
  • BioVision's rat IL-6 ELISA Kit is based on the standard sandwich enzyme-linked immunosorbent assay technology. (biovision.com)
  • Sandwich ELISA analysis of mouse IFN-γ protein using GTX02927 Interferon gamma antibody [AN18] as coating antibody and GTX02943-02 Interferon gamma antibody [R4-6A2] (Biotin) as detecting antibody. (genetex.com)
  • This ELISA kit uses the Sandwich-ELISA principle. (caslab.com)
  • Enzyme-linked immunosorbent assay (ELISA) is either based on sandwich, competitive, or inhibition type of format. (bvsalud.org)
Reactivity1
Solution13
  • The substrate is supplied as a ready to use, mildly acidic solution. (cloverbiosciences.com)
  • Working solution shelf life - Substrates are always best made up fresh, but if you're throwing working solution away, a different formulation may help minimize wastage. (2bscientific.com)
  • Increase Substrate Solution incubation time. (stratech.co.uk)
  • After TMB substrate solution is added, only those wells that contain Androgen Receptor (AR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. (avivasysbio.com)
  • The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. (avivasysbio.com)
  • These ELISA test kits are the ideal solution for an accurate measurement of multiple samples in a very broad quantitation range. (romerlabs.com)
  • After a further washing step, the substrate (TMB) solution is pipetted and incubated for 20 minutes, inducing the development of a blue dye in the wells. (rapidtest.com)
  • Substrate Solution : One 12 mL amber bottle. (antibodies-online.com)
  • Warm Substrate Solution to room temperature. (antibodies-online.com)
  • Add 100 μL of Substrate Solution to each well, including the blank wells. (antibodies-online.com)
  • The substrate solution is added to each well. (caslab.com)
  • The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. (caslab.com)
  • Simoa uses the same reagents as conventional ELISA, but antibody capture agents are attached to the surface of paramagnetic beads that are used to concentrate a dilute solution of target molecules. (lu.se)
Protein3
  • Because a limited amount of EPO products was available, we were restricted to the common assays used for quality control: high performance size exclusion chromatography (HP-SEC) to assess monomer and aggregate content, ELISA to determine EPO protein content, SDS-PAGE under non-reducing conditions to detect possible fragmentation and covalent protein aggregates, capillary zone electrophoresis (CZE) for isoform profiling and a normocythemic mouse assay to test for potency. (springer.com)
  • They have the ability to modulate protein activity by binding to a target protein inside cells to prevent protein-protein interactions, disrupt protein-nucleic acid interactions, or prevent substrate access to enzymes 1-5 . (jove.com)
  • We found this overlap to be largely attributable to the accumulation of high-temperature requirement protein A1 (HTRA1), a serine protease with an established role in the brain vasculature, and several of its substrates. (biomedcentral.com)
Microplate1
  • The ELISA technique has become a popular standard technique through the microplate format, from R&D to QC and Diagnostics. (interchim.com)
Chromatography1
  • Two original products, Eprex (epoetin alfa) and Dynepo (epoetin delta), and two biosimilar products, Binocrit (epoetin alfa) and Retacrit (epoetin zeta), were compared using (1) high performance size exclusion chromatography, (2) ELISA, (3) SDS-PAGE, (4) capillary zone electrophoresis and (5) in-vivo potency. (springer.com)
Quantitation1
  • OxiSelect™ Oxidative UV-induced DNA Damage ELISA Kit (CPD Quantitation) is an enzyme immunoassay developed for rapid detection and quantitation of CPD in any DNA samples. (antibodies-online.com)
Reagents2
  • In ELISA, a liquid sample is added onto a stationary solid phase with special binding properties and is followed by multiple liquid reagents that are sequentially added, incubated, and washed, followed by some optical change (e.g., color development by the product of an enzymatic reaction) in the final liquid in the well from which the quantity of the analyte is measured. (wikipedia.org)
  • BioScience Innovations provide any kind of reagents, and aims to select the best source of conventional reagents (buffers, saturating agents, ELISA substrates,…), as well as to promote great specific, original and powerful solutions. (interchim.com)
Supernatants1
  • Mouse CD26 (DPPIV) ELISA Kit for cell culture supernatants, plasma, and serum samples. (antikoerper-online.de)
Conventional1
  • The limit of detection of this study was 24.3 PFU/ml, a limit 116 times lower than that of conventional ELISA (2824.3 PFU/ml). (nature.com)
Assay Procedure1
  • Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided. (rndsystems.com)
Qualitative2
  • Neogen's Theophylline ELISA (Enzyme-Linked ImmunoSorbent Assay) kit is a qualitative one-step kit designed for use as a screening test for the detection of drugs and/or their metabolites in equine or canine matrices. (neogen.com)
  • This ELISA test is a qualitative serological assay for the detection of Kyuri green mottle mosaic virus (KGMMV) in cucurbit leaves. (agdia.com)
Plate4
  • Unlike other spectrophotometric wet lab assay formats where the same reaction well (e.g., a cuvette) can be reused after washing, the ELISA plates have the reaction products immunosorbed on the solid phase, which is part of the plate, and so are not easily reusable. (wikipedia.org)
  • The objective of this studywas to evaluate diagnostic performance and discriminative ability of Rose Bengal Plate Test (RBPT), complement fixation test (CFT), and indirect enzyme linked immunosorbent assay (I-ELISA) tests used for screening and confirmatory diagnosis of bovine brucellosis in Ethiopia using Bayesian method. (hindawi.com)
  • The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rabbit MMP-13. (caslab.com)
  • Samples (or Standards) are added to the micro ELISA plate wells and combined with the specific antibody. (caslab.com)
Kits4
  • The assay kits will no longer include ELISA microwell plates. (biolegend.com)
  • BioLegend's mouse and human IL-1ß ELISA kits use antibodies raised against the mature form of IL-1ß. (biolegend.com)
  • The human IL-1ß ELISA kits have been shown to equally recognize both pro- and mature forms of IL-1ß. (biolegend.com)
  • For more information about ELISA Kits, Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representative at 818-591-3030 . (rapidtest.com)
ECIS1
  • The addition of microglia or ATP led to the disruption of the MBEC monolayer and significantly decreased barrier function as measured by trans-endothelial electrical resistance (TEER) and electric cell-substrate impedance sensing (ECIS). (portlandpress.com)
Products1
Serum and plasma2
  • Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA. (rndsystems.com)
  • Both the ELISA Max™ Set Standard and ELISA Max™ Set Deluxe are specifically engineered for accurate recovery and measurement of analytes in complex fluids, such as serum and plasma (as well as activated cell cultures) without any interference by confounding serum factors, such as heterophilic antibody or rheumatoid factor. (biolegend.com)
Commercially available2
  • Unlike other commercially available substrates, Bio-Helix's TMB contains no DMF. (cloverbiosciences.com)
  • Test evaluation in the absence of a gold standard test was conducted for the diagnosis and screening of bovine brucellosis using three commercially available tests including RBPT, CFT, and I-ELISA in National Animal Health Diagnostic and Investigation Center (NAHDIC) Ethiopia. (hindawi.com)
Incubation1
  • Study doctors verifi ed 405 nm after a fi nal incubation with AP substrate (15 min, eligibility and willingness to return for convalescent-phase room temperature, in the dark). (cdc.gov)
Reacts2
  • The substrate reacts with __, causing a color change which indicates whether or not the desired substance was present in the original sample. (submissionstatus.com)
  • Based on immunoelectrophoresis and/or ELISA, the antibody reacts with the Fc portion of mouse IgG heavy chain but not with the Fab portion of mouse immunoglobulins. (jacksonimmuno.com)
Enzyme-linked3
Enzymatic2
  • HRP substrate TMB is used to visualize HRP enzymatic reaction. (abcam.com)
  • In wells containing a bead with a single target molecule, the formation of an immunocomplex will result in enzymatic cleavage of a substrate, which will be converted to a fluorescent product imaged by the SR-X reader. (lu.se)
Laboratories2
  • All Vector Laboratories' substrates are provided in liquid format to minimise exposure and enable run appropriate volumes to be made for bench top runs, or on an autostasiner for suitable substrates. (2bscientific.com)
  • Vector Laboratories offers two lines of peroxidase substrates. (2bscientific.com)
Assays1
  • Of note, ELISA can perform other forms of ligand binding assays instead of strictly "immuno" assays, though the name carried the original "immuno" because of the common use and history of development of this method. (wikipedia.org)
Test5
  • The Elisa test is a common laboratory technique used to detect the presence of certain substances in a sample. (submissionstatus.com)
  • Diagnostic Automation HSV1 IgA elisa test kit is intended for use in evaluating a patient's serologic status to Herpes Simplex Virus (HSV) infection, or for evaluating paired sera for the presence of a significant increase in herpes specific IgA. (rapidtest.com)
  • HSV1 IgA elisa test kit is an accurate serologic method to detect HSV specific antibody in serum sample. (rapidtest.com)
  • Antibody was detected by successive incubations with test sera, urease-conjugated rabbit-human antibody, and urease substrate. (ajtmh.org)
  • The Wampole Measles IgG ELISA test system is designed to detect IgG class antibodies to Measles virus in human sera. (cdc.gov)
Reproducibility1
Immunohistochemistry1
  • Peroxidase conjugates are commonly used for immunohistochemistry, Western blotting, and ELISA. (jacksonimmuno.com)
Component1
  • As a heterogenous assay, ELISA separates some component of the analytical reaction mixture by adsorbing certain components onto a solid phase which is physically immobilized. (wikipedia.org)
Diagnostic1
  • ELISA has been used as a diagnostic tool in medicine, plant pathology, and biotechnology, as well as a quality control check in various industries. (wikipedia.org)
Antibodies directed1
  • The ELISA method is based on the use of antibodies directed against alachlor. (cdc.gov)