GFAPSandwich-ELISAAstrocytesAntibodyDetectionEnzyme-linkeSerumMonocyteReactivityS100HomogenatesAntibodiesExtracellularConcentrationsPlasmaSpinalAmyloid precursTitersCytokinesAutoantibodiesMRNATissuesCellsAntigensImmunohistochemistryImmunofluorescenceKinaseInflammationPhosphorylationBrainImmuneSpeciesEncodesExpressionLevelsDiseases
GFAP14
- This ELISA kit applies to the in vitro quantitative determination of Mouse GFAP concentrations in serum, plasma and other biological fluids. (elabscience.com)
- The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse GFAP. (elabscience.com)
- Plasma concentrations of tau, glial fibrillary acidic protein (GFAP), ubiquitin carboxyl-terminal hydrolase L1, and neurofilament light chain (NFL) were measured using the single-molecule array technology. (quanterix.com)
- Using ELISA spinal tissue exhibited increased albumin, glial fibrillary acidic protein (GFAP), myelin basic protein (MBP) and heat shock protein (HSP 72kD) upregulation together with cytokines TNF-α, IL-4, IL-6, IL-10 levels in nerve lesion that was exacerbated following NPs intoxication. (iasp-pain.org)
- Recognizes the ~55 kDa glial fibrillary acidic protein (GFAP). (scientificlabs.co.uk)
- The aim of the study was to measure glial fibrillary acidic protein (GFAP) as a marker of brain damage in the serum of patients that underwent internal carotid endarterectomy (CEA). (viamedica.pl)
- Serum GFAP levels were measured by a commercially available enzyme-linked immunosorbent assay (ELISA). (viamedica.pl)
- WB detected MGCs activation marker glial fibrillary acidic protein (GFAP) protein levels. (bvsalud.org)
- Studies of glial fibrillary acidic protein (GFAP), an astrocytic damage marker, may help advance our understanding of different neurodegenerative diseases. (biomedcentral.com)
- Compared with their wild-type counterparts, IL-10 tm1/tm1 astrocytes exhibited higher expression of glial fibrillary acidic protein (GFAP). (biomedcentral.com)
- exploring the role of plasma glial fibrillary acidic protein (GFAP) in PD with cognitive impairment has shed new light on this important area of study. (geneworks.com.au)
- GFAP is an astrocyte-specific intermediate filament protein that plays a crucial role in maintaining the structural integrity and functioning of astrocytes, the star-shaped glial cells in the central nervous system. (geneworks.com.au)
- GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. (affbiotech.com)
- For example, the CSF interleukin (IL)-6 levels in NMO patients were significantly higher compared to those in patients with MS or other non-inflammatory neurological disorders, and were significantly correlated with clinical variables, including the Expanded Disability Status Scale (EDSS) score, CSF glial fibrillary acidic protein (GFAP) level and anti-AQP4 antibody titers [15C17]. (innovation-ecosystems-agora.com)
Sandwich-ELISA3
- This ELISA kit uses the Sandwich-ELISA principle. (elabscience.com)
- Quantification of Glial Fibrillary Acidic Protein: Comparison of Slot-Immunobinding Assays with a Novel Sandwich ELISA. (epa.gov)
- Description: A sandwich ELISA kit for detection of Thrombospondin 1 from Mouse in samples from blood, serum, plasma, cell culture fluid and other biological fluids. (ksiazkiwnauce.pl)
Astrocytes6
- The functional phenotype of astrocytes was determined by quantitative RT-PCR, ELISA, and immunohistochemistry. (biomedcentral.com)
- Astrocytes comprise the most abundant glial cells in the mammalian brain. (biomedcentral.com)
- Furthermore, neuropathologists have found that glial cells, including astrocytes and microglia, are activated in each of these disorders, accompanied by low-to-moderate levels of inflammatory mediators in the parenchyma [ 16 ]. (biomedcentral.com)
- In response to released cytokines from immune cells, perilesional astrocytes modify their phenotype and form the glial scar composed of astrocytes and a dense extracellular matrix within weeks following SCI [ 1 ]. (biomedcentral.com)
- This gene encodes one of the major intermediate filament proteins of mature astrocytes. (affbiotech.com)
- It is used as a marker to distinguish astrocytes from other glial cells during development. (affbiotech.com)
Antibody4
- Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. (elabscience.com)
- Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Surfactant Associated Protein C (SPC) in Tissue homogenates, cell lysates, lung lavage fluid and other biological fluids. (stemcellcharter.org)
- Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Protein Disulfide Isomerase (PDI) in serum, plasma, tissue homogenates and other biological fluids. (knoblauchpublishing.com)
- Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Protein Disulfide Isomerase (PDI) in samples from serum, plasma, tissue homogenates and other biological fluids with no significant corss-reactivity with analogues from other species. (knoblauchpublishing.com)
Detection8
- This Anti-Glial Fibrillary Acidic Protein Rat mAb (2.2B10) is validated for use in ELISA, Immunoblotting, Frozen Sections, IP, Paraffin Sections for the detection of Glial Fibrillary Acidic Protein. (scientificlabs.co.uk)
- In the present study TMT was used to assess the detection of the serum polyclonal IgG responses against NFs, measured by ELISA, as a peripheral marker of neurotoxicity. (cdc.gov)
- This study suggests that the detection of autoantibodies to neurotypic proteins, using ELISA, may be used to indicate chemical neurotoxicity. (cdc.gov)
- Description: A sandwich quantitative ELISA assay kit for detection of Mouse Thrombospondin 1 (THBS1) in samples from plasma, tissue homogenates or other biological fluids. (ksiazkiwnauce.pl)
- Description: A sandwich quantitative ELISA assay kit for detection of Human Surfactant Associated Protein C (SPC) in samples from serum, plasma, tissue homogenates, cell lysates, lung lavage fluid, cell culture supernates or other biological fluids. (stemcellcharter.org)
- Description: A sandwich quantitative ELISA assay kit for detection of Human Protein Disulfide Isomerase (PDI) in samples from serum, plasma, tissue homogenates or other biological fluids. (knoblauchpublishing.com)
- WB: For western blot detection of denatured protein samples. (affbiotech.com)
- ELISA(peptide): For ELISA detection of antigenic peptide. (affbiotech.com)
Enzyme-linke1
- Enzyme-linked immunosorbent assay (ELISA) detected IL-11 levels in MIO-M1 cell culture supernatant. (bvsalud.org)
Serum3
- Description: A competitive ELISA for quantitative measurement of Mouse Glutamate Receptor, Ionotropic, N Methyl D Aspartate 2C in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. (stemcellcharter.org)
- Description: Quantitativesandwich ELISA kit for measuring Human protein disulfide isomerase, PDI in samples from serum, plasma, tissue homogenates. (knoblauchpublishing.com)
- This Rat Hyaluronic acid (HA) ELISA Kit was designed for the quantitative measurement of Rat Hyaluronic acid (HA) protein in serum, plasma, tissue homogenates. (cusabio.com)
Monocyte1
- Other cytokines/chemokines including interleukin (IL)-6 and monocyte chemotactic protein (MCP)-1 were also significantly increased in the acute NMOSD group compared to both the MS and HC groups. (innovation-ecosystems-agora.com)
Reactivity3
- We sought to determine whether immune reactivity occurs between anti-SARS-CoV-2 protein antibodies and human tissue antigens, and whether molecular mimicry between COVID-19 viral proteins and human tissues could be the cause. (frontiersin.org)
- Cross-reactivity occurs when amino acid sequence homology exists between a pathogen and self-tissue proteins ( 1 ). (frontiersin.org)
- In this mechanism, antibodies formed against SARS-CoV-2 would also bind to human tissue proteins leading to autoimmune reactivity. (frontiersin.org)
S1001
- S-100B protein is a member of highly homologous acidic calcium and zinc binding proteins family (S100 family) that possess two EF-hand motifs. (biovendor.com)
Homogenates2
- Western-blots, using purified NF proteins, or hippocampal homogenates, were used to confirm ELISA. (cdc.gov)
- Immunostaining of Western-blots using HRP-conjugated anti-rat IgG confirmed the presence of antibodies, against purified proteins or hippocampal homogenates, in sera of rats exposed to TMT. (cdc.gov)
Antibodies3
- We applied both human monoclonal anti-SARS-Cov-2 antibodies (spike protein, nucleoprotein) and rabbit polyclonal anti-SARS-Cov-2 antibodies (envelope protein, membrane protein) to 55 different tissue antigens. (frontiersin.org)
- We found that SARS-CoV-2 antibodies had reactions with 28 out of 55 tissue antigens, representing a diversity of tissue groups that included barrier proteins, gastrointestinal, thyroid and neural tissues, and more. (frontiersin.org)
- Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. (affbiotech.com)
Extracellular3
- Guha D, Mukerji SS, Chettimada S, Misra V, Lorenz DR, Morgello S, Gabuzda D. Cerebrospinal fluid extracellular vesicles and neurofilament light protein as biomarkers of central nervous system injury in HIV-infected patients on antiretroviral therapy. (biovendor.com)
- Two well-defined pathological hallmarks of AD have been described: the formation of extracellular amyloid plaques and the development of intracellular neurofibrillary tangles (NFTs) formed by aggregated hyperphosphorylated TAU protein [ 4 ]. (biomedcentral.com)
- Immunohistochemistry revealed a marked increase in extracellular clusterin protein expression in the perilesional cortex and ipsilateral hippocampus (7d to 1 month post-TBI), and ipsilateral thalamus (14d to 12 months post-TBI). (nature.com)
Concentrations1
- Relationships between Accumulation of a 60 kDa Stress Protein and Scope-for-Growth in 'Mytilus edulis' Exposed to a Range of Copper Concentrations. (epa.gov)
Plasma1
- On this basis, we hypothesized that TBI leads to increases in the brain levels of clusterin protein, and consequently, increased plasma clusterin levels. (nature.com)
Spinal1
- Apart from inflammation, the formation of a glial scar is one of the most prominent host reactions after injury of the CNS including the spinal cord. (biomedcentral.com)
Amyloid precurs2
- Additionally, we detected increased levels of active glycogen synthase kinase 3 β, a physiological kinase of TAU, in neurons derived from AD iPSCs, as well as significant upregulation of amyloid precursor protein (APP) synthesis and APP carboxy-terminal fragment cleavage. (biomedcentral.com)
- Most cases of early-onset AD are linked to autosomal dominant inherited mutations in the genes encoding amyloid precursor protein ( APP ), presenilin 1 ( PSEN1 ), and presenilin 2 ( PSEN2 ). (biomedcentral.com)
Titers1
- The polyclonal IgG response to NF68, NF160, and NF200, using ELISA, showed detectable titers of IgG autoantibodies to NFs in sera from TMT-exposed rats, only. (cdc.gov)
Cytokines1
- Phosphorylation of NMDA receptor and expression of inflammatory cytokines were examined with Western blot, real-time RT-PCR and ELISA. (biomedcentral.com)
Autoantibodies3
- Autoantibodies to nervous system proteins following trimethyl tin (TMT) exposure: a comparison of ELISA and Western-blot analysis. (cdc.gov)
- In more than 80% of cases, NMO is caused by immunoglobulin G autoantibodies to aquaporin 4 (anti-AQP4), the most abundant water channel protein in the central nervous system. (wikipedia.org)
- citation needed] NMOSD is usually caused by autoantibodies targeting aquaporin 4 (AQP4), a channel protein in the cell membrane that allows water to pass through the membrane. (wikipedia.org)
MRNA3
- QRT-PCR and WB detected tight junction-associated molecules claudin-5, occluding and tight junction protein 1 (ZO-1) mRNA and protein levels in mouse retina tissues, respectively. (bvsalud.org)
- Despite the proposed role for nerve growth factor (NGF) in this sprouting, we observed no change in NGF mRNA or protein at several postlesion time points. (jneurosci.org)
- Pansensitive and panresistant genes to 21 NCCN-recommended drugs with concordant mRNA and protein expression were identified. (cdc.gov)
Tissues2
- ELISA detected IL-11 and IL-11RA levels in mouse retina tissues. (bvsalud.org)
- We believe the answer is probable, since some of the systemic disease clinical manifestations of COVID-19 cannot be explained solely by the binding of SARS-CoV-2 spike proteins with cell membranes of tissues that exhibit angiotensin-converting enzyme 2 (ACE2). (frontiersin.org)
Cells7
- Protein phosphorylation is a key regulatory mechanism in eukaryotic cells. (chemcarbindia.com)
- This analysis indicated that ZnO NPs can transfer into the CNS, activate glial cells and cause neuroinflammation after tongue instillation. (biomedcentral.com)
- In normal cells the BRCA1 protein is localized in the nucleus, whereas in the majority of breast cancer cell lines and in malignant pleural effusions from breast cancer patients, it is localized mainly in the cytoplasm. (lookformedical.com)
- Many neoplasm proteins have been characterized and are used as tumor markers (BIOMARKERS, TUMOR) when they are detectable in cells and body fluids as monitors for the presence or growth of tumors. (lookformedical.com)
- Results The induced protein (IP)-10 level in the cells was markedly increased following exposure to acute phase NMOSD sera. (innovation-ecosystems-agora.com)
- The up-regulation of the IP-10 levels in the cells after exposure to the acute-phase NMOSD sera was also observed using another specified ELISA, and this effect was significantly decreased during the remission phase in the individual NMOSD patients. (innovation-ecosystems-agora.com)
- The primary barriers for invading respiratory pathogens are the respiratory tract epithelial cells and antimicrobial proteins generated by these cells. (cdc.gov)
Antigens2
- We also did selective epitope mapping using BLAST and showed similarities and homology between spike, nucleoprotein, and many other SARS-CoV-2 proteins with the human tissue antigens mitochondria M2, F-actin and TPO. (frontiersin.org)
- Some neoplasm proteins are tumor antigens (ANTIGENS, NEOPLASM), i.e. they induce an immune reaction to their tumor. (lookformedical.com)
Immunohistochemistry1
- Then, murine behaviors were tested, and the hippocampus and cortex were analyzed by quantitative RT-PCR, ELISA, and immunohistochemistry. (biomedcentral.com)
Immunofluorescence1
- WB detected IL-RA protein levels, and Immunofluorescence measured the co-localization of IL-11 and IL-11RA. (bvsalud.org)
Kinase3
- Acquisition and Loss of a Neuronal Ca(2+)/Calmodulin-Dependent Protein Kinase during Neuronal Differentiation. (epa.gov)
- Nuclear and Axonal Localization of Ca(2+)/Calmodulin-Dependent Protein Kinase Type Gr in Rat Cerebellar Cortex. (epa.gov)
- Based on our identified miRNA-regulated molecular machinery, an inhibitor of PDK1/Akt BX-912, an anthracycline antibiotic daunorubicin, and a multi-targeted protein kinase inhibitor midostaurin were discovered as potential repositioning drugs for treating lung cancer. (cdc.gov)
Inflammation1
- Because of its favorable safety profile and the involvement of misfolded proteins, oxidative damage, and inflammation in multiple chronic degenerative diseases, these data relating curcumin dosing to the blood and tissue levels required for efficacy should help translation efforts from multiple successful preclinical models. (aspetjournals.org)
Phosphorylation1
- Neurons from patients with fAD and patients with sAD showed increased phosphorylation of TAU protein at all investigated phosphorylation sites. (biomedcentral.com)
Brain1
- In addition, increased levels of 4-hydroxynonenal and nitrotyrosine were measured in cerebella of HFD ± NDEA treated rats, and overall, NDEA+HFD treatment reduced brain levels of Tau, phospho-GSK-3β (reflecting increased GSK-3β activity), glial fibrillary acidic protein, and ChAT to greater degrees than either treatment alone. (biomedcentral.com)
Immune1
- Additionally, such immune response modulation could have impacted astrocyte activation resulting in a reduced glial scar. (biomedcentral.com)
Species1
- This is a high quality ELISA kit developped for optimal performance with samples from the particular species. (stemcellcharter.org)
Encodes2
- It encodes a large nuclear protein that is a component of DNA repair pathways. (lookformedical.com)
- It encodes a large, nuclear protein that is an essential component of DNA repair pathways, suppressing the formation of gross chromosomal rearrangements. (lookformedical.com)
Expression2
- Proteins whose abnormal expression (gain or loss) are associated with the development, growth, or progression of NEOPLASMS. (lookformedical.com)
- Abnormal expression of ONCOGENE PROTEINS is involved in neoplastic transformation, whereas the loss of expression of TUMOR SUPPRESSOR PROTEINS is involved with the loss of growth control and progression of the neoplasm. (lookformedical.com)
Levels1
- Paraffin-embedded sections were used to assess p65 NF-κB nuclear translocation at days 1, 3, 7, 10, 14, and 28 and to determine levels of glial scaring, white and gray matter preservation, and cavity size at day 28 after SCI. (biomedcentral.com)
Diseases1
- A biomarker that has been involved in neuronal diseases but more recently involved in Stroke is Parkinson Protein 7 (PARK7). (randoxbiosciences.com)