Elegans embryos. Medical search. Frequent questions

We showed that QCANet can be applied not only to developing mouse embryos but also to developing embryos of two other model species. (nature.com)
Using QCANet, we were able to extract several quantitative criteria of embryogenesis from 11 early mouse embryos. (nature.com)
The volumetric analysis of cellular expression patterns provides evidence for molecular mechanisms regulating the development of pre-implantation mouse embryos. (zeiss.com)
1-6 These platforms provide sectional imaging of cells, microorganisms and model animals such as C. elegans , zebrafish and mouse embryos. (jove.com)
A selection of evaluations from Faculty of 1000 covering a description of the human plasma proteome, the C. elegans interactome, a primitive Y chromosome in papaya, a robot scientist and the expression profiling of preimplantation mouse embryos. (biomedcentral.com)

Drosophila embryos are easily amenable to imaging because they are more transparent than the embryos of other model organisms, such as mice. (nature.com)
The manual features primers on live imaging of a variety of standard model organisms including C. elegans, Drosophila, zebrafish, Xenopus, avian species, and mouse. (cshlpress.com)
We demonstrate the performance of our approach on data acquired from living embryos of Drosophila and fixed adult C.elegans worms. (sri.com)
Confocal micrograph of a Drosophila embryo stained for Pax3/7 (cyan), eve, horse radish peroxidase (red) and counterstained with DAPI (blue). (cellimagelibrary.org)

Confocal micrograph of a transgenic zebrafish embryo at 24 hours post-fertilization, showing expression of the fusion protein PARD3_GFP. (cellimagelibrary.org)

The one-cell C. elegans embryo serves as a model for studying the establishment and maintenance of polarity. (biologists.com)
We have found that the C. elegans homolog of Lgl, LGL-1, has a role in polarity but is not essential. (biologists.com)
It localizes asymmetrically to the posterior of the early embryo in a PKC-3-dependent manner, and functions redundantly with PAR-2 to maintain polarity. (biologists.com)
We used this method to measure microtubule polarity throughout the first mitotic spindle in C. elegans embryos. (harvard.edu)

Our laboratory uses high-throughput functional genomics and computational approaches to study the cell biology, post-translational regulation, and evolution of molecular mechanisms during early embryonic development in the simple animal model C. elegans and related nematodes. (nyu.edu)
I subsequently applied for a position in Dr. Rebecca Lyczak's C. elegans research lab to learn about early embryonic development. (ursinus.edu)

Where researchers once struggled to connect events at static timepoints, imaging tools now offer the ability to visualize the dynamic form and function of molecules, cells, tissues, and whole embryos throughout the entire developmental process. (cshlpress.com)
The production of viable offspring requires three key developmental events: oocyte maturation, the oocyte-to-embryo transition (OET), and the maternal-to-zygotic transition (MZT) ( Figure 1A ). (elifesciences.org)
Developmental dynamics of gene expression and alternative polyadenylation in the Caenorhabditis elegans germline. (nyu.edu)
Therefore, anyone with an interest in cell and developmental biology, regardless of their area of specialisation, would benefit from reading C. elegans II . (cshlpress.com)
Microscopy allows me to observe developmental timepoints and possible defects in the embryos, specifically during the first division. (ursinus.edu)
To prevent any aberrant changes in the developmental phenotype, the embryos could not be could not be forced against the cover glass and needed to be imaged quickly and with low phototoxicity. (zeiss.com)
Recently, the three-dimensional time-lapse live imaging and delineated developmental programs in the roundworm Caenorhabditis elegans provide an excellent platform for establishing quantitative models. (aps.org)
Transcriptomic analyses provide an efficient approach to explore the temporal gene expression profiles in embryos and to search for the developmental regulators. (biomedcentral.com)

Studies of the cells and genes of the nematode C. elegans have become a cornerstone of current biology. (cshlpress.com)
A classic 1988 Cold Spring Harbor monograph, The Nematode Caenorhabditis elegans, described the basic genetics, anatomy and development of the organism. (cshlpress.com)
The chapters are more focused and comprehensive than those found in its predecessor (the 1988 monograph, The Nematode Caenorhabditis elegans ). (cshlpress.com)
Many new chapters have been included which deal with topics that were accorded only a few lines in the original, such as lifespan and ageing, nematode evolution and the relationship of C. elegans to parasitic nematodes. (cshlpress.com)
In C. elegans, lon-8 is involved in nematode male tail tip morphogenesis and regulation of growth. (wormbase.org)
Specifically, they are using the early embryo of the soil nematode, Caenorhabditis elegans, as a model system because of the advantages it offers for the molecular analysis of mitosis. (pewtrusts.org)

Spermatogenesis produces haploid sperm capable of penetrating the oocyte, whereas oogenesis produces differentiated oocytes that are stockpiled with maternal nutrients, proteins, and mRNAs, and have outer layers that protect the embryo and enable fertilization. (elifesciences.org)
In the early embryo, a myosin II-dependent contraction of the cortical meshwork asymmetrically distributes the highly conserved PDZ proteins PAR-3 and PAR-6, as well as an atypical protein kinase C (PKC-3), to the anterior. (biologists.com)
Localization of the proteins in the embryo helps with our understanding of their role during development. (ursinus.edu)
PARD proteins, which were first identified in C. elegans, are e. (cellimagelibrary.org)
Transcripts found in unfertilized oocytes also encoded a large number of proteins implicated in cell adherence, tight junction and focal adhesion, suggesting high complexity in terms of structure and cellular interactions in embryos prior to midblastula transition (MBT). (biomedcentral.com)

Here we combine the first large-scale serial electron tomography of whole mitotic spindles in early C. elegans embryos with live-cell imaging to reconstruct all microtubules in 3D and identify their plus- and minus-ends. (nature.com)
In this paper, we set out to identify the cytoskeletal ultrastructure in C. elegans mitotic spindles that underlies this function, and how this ultrastructure is generated, using a combination of large-scale electron tomography, light microscopy and mathematical modelling. (nature.com)
Furthermore, we have investigated the mechanism of chromosome segregation in C. elegans mitotic spindles, human mitotic spindles, and C. elegans female meiotic spindles. (harvard.edu)

Large-scale sorting of C. elegans embryos reveals the dynamics of small RNA expression. (mpg.de)
The PAM-1 aminopeptidase interacts with cytoskeletal regulators in controlling cortical dynamics in the one-cell C. elegans embryo. (ursinus.edu)

For example, since most diseases are multigenic in origin, we are generating genome-wide genetic interaction maps for genes with essential roles in the early embryo that will help us understand how these interactions affect embryogenesis. (nyu.edu)
RNAi is used to knock out the gene of interest (the two genes I am interested in are ani-1 and nmy-2) , resulting in the embryos not having the protein that gene encodes. (ursinus.edu)
The database stores data from recent systematic studies of cell lineage differentiation in the C. elegans embryo containing ∼ 200 conserved genes, 1400 perturbed cell lineages and 600,000 digitized single cells. (nih.gov)

Morphogenesis is important for creating the three germ layers of the early embryo (ectoderm, mesoderm, and endoderm) and for building up complex structures during organ development. (wikipedia.org)
The foxa gene is an integral component of the endoderm specification subcircuit of the endomesoderm gene regulatory network in the Strongylocentrotus purpuratus embryo. (biologists.com)

During Genetics Lab my Sophomore year, one of our first experiments was using C. elegans to characterize phenotypic defects and determine the mutation. (ursinus.edu)

Because maturing oocytes and early embryos lack appreciable transcription, posttranscriptional regulatory processes control their development. (elifesciences.org)
The analyses were carried out in unfertilized oocytes, newly fertilized oocytes and embryos at the stages of mid-blastula transition and segmentation. (biomedcentral.com)

Thus, embryo development is highly dynamic. (nature.com)
Recording and contextualizing the science of embryos, development, and reproduction. (asu.edu)
The endomesoderm gene regulatory network (GRN) models the transcriptional control system defining vegetal specification of the sea urchin( Strongylocentrotus purpuratus ) embryo during the first 30 hours of development. (biologists.com)
Wnt signaling has been demonstrated to regulate diverse cell processes throughout the development of the Caenorhabditis elegans embryo. (nih.gov)
Now, in that authoritative tradition, comes C. elegans II -- not a second edition but a book that breaks new ground and defines the current status of the field, providing a detailed molecular explanation of how development is regulated and the nervous system specifies varied aspects of behavior. (cshlpress.com)
His research interests are in the mechanisms involved in the development of the embryo. (webofstories.com)
A landmark early example of using phylogenetics to describe cellular relationships was that of Sulston and colleagues reporting the development of C. elegans as deduced from meticulous visual observation [ 1 , 2 ]. (biorxiv.org)
In C. elegans, rpn-8 is involved in programmed cell death involved in cell development. (wormbase.org)
Today, it is generally thought that the combination of determinants deposited by the mother during oogenesis and the inductive signals between different cells trigger the specification of different cell lineages during development of the embryo [ 7 , 8 ]. (biomedcentral.com)

Anterior and posterior regions of a C. elegans embryo are determined by PAR protein distribution. (cellimagelibrary.org)
PAR-2, shown in green marks the posterior region of the embryo and PAR-6, shown in red, marks the ante. (cellimagelibrary.org)
A higher concentration of Eph transcripts is evident in the posterior pole of the embryo. (sdbonline.org)

In the embryo, zygotic transcription of Eph is confined to the nervous system. (sdbonline.org)

To determine which cells are expressing specific cellular markers and how these cells are organized relative to each other requires volumetric imaging of the pre-implantation embryos by confocal microscopy. (zeiss.com)

Because the cells in embryos are considerably crowded, an algorithm to segment individual cells in detail and accurately is needed. (nature.com)
In early embryos, cells are loosely connected to each other. (nature.com)
At the 8-cell stage, the embryo becomes compact, and the cells form a spherical mass called a morula. (nature.com)
For example, in the neural plate of the embryo this system operates to generate a population of neuronal precursor cells in which NeuroD is highly expressed. (wikipedia.org)
Further techniques are organized by the level of visualization they provide, from cells to tissues and organs to whole embryos. (cshlpress.com)
These cells are also sometimes referred to as lateral hypodermal cells, because they share some functions with the major hypodermis, including secreting cuticle and generating the contractile force during elongation of the embryo. (wormatlas.org)
SeamFIG 1: Seam is composed of a longitudinal string of cells on each side of C. elegans . (wormatlas.org)
During the transition from the naive epiblast to a pluripotent state, over 100 cells are organized into an approximately 60-80 µm diameter pre-implantation mouse embryo. (zeiss.com)
Patterns are ubiquitous in living systems and underlie the dynamic organization of cells, tissues, and embryos. (mpi-cbg.de)

This volume is a must for any investigator doing worm studies but it has been written and rigorously edited to illuminate for a wider community of investigators in cell and molecular biology who should know how new knowledge of C. elegans relates to their own specialty. (cshlpress.com)

I use RNAi (RNA interference) for gene knock-out and then image the embryos through two types of microscopy to analyze the interactions of cytoskeletal regulators with the PAM-1 aminopeptidase in organizing the cytoskeleton in the one-cell C. elegans embryo. (ursinus.edu)

In quick succession, the preimplantational mouse embryo undergoes a series of cellular divisions involving two critical cell fate decisions. (zeiss.com)

Here, by using genome editing and depletion of tubulin isotypes following RNAi, we demonstrate that four tubulin isotypes (hereafter referred to as α1, α2, β1 and β2) cooperatively confer distinct MT properties in Caenorhabditis elegans early embryos. (elsevierpure.com)

2018) Neurexin directs partner-specific synaptic connectivity in C. elegans. (assumption.edu)

Caenorhabditis elegans represents a valuable model for investigating ACD, given its invariant cell lineage and conserved mechanisms of ACD. (elifesciences.org)
As with all model systems, a great deal of what is learned from C. elegans is likely to be applicable to other organisms. (cshlpress.com)
In this paper, we study two popular computational models for multicellular systems, i.e., the phase-field model and the coarse-grained model, and compare their performance in characterizing the cell morphologies, cell adhesion, and cell stiffness in a real C. elegans embryo. (aps.org)
As a graduate student at Cornell University, she employed the invertebrate model organism C. elegans, and made significant contributions to the discovery of RNA interference (RNAi) as well as understanding of asymmetric cell divisions. (ucsf.edu)

Indeed, that such forces exist in C. elegans is evidenced by the maintenance of half-spindle lengths throughout mitosis 5 and in many perturbations experiments. (nature.com)
The experiments are done in both wildtype (normal) embryos and pam-1 mutant embryos. (ursinus.edu)
Lecture and textbook material introduced me to experiments, but nothing could have conveyed the excitement and awe I felt watching that first division of the one cell C. elegans embryo. (ursinus.edu)
An inquiry-based approach to study the synapse: student-driven experiments using C. elegans. (assumption.edu)
CherryTemp allows us to perform temperature shift experiments with unprecedented speed and precision with C. elegans embryos. (cherrybiotech.com)

Here, we demonstrate that the nucleosome remodeling and deacetylase (NuRD) complex is asymmetrically segregated into the surviving daughter cell rather than the apoptotic one during ACDs in Caenorhabditis elegans . (elifesciences.org)

eph dsRNA was injected into syncytial stage embryos to perturb eph expression at the larval time points relevant to axon targeting in the adult visual system. (sdbonline.org)
CherryTemp was the perfect solution for us to combine precisely controlled temperature stimuli and live cell imaging in our functional studies on thermosensory neurons in C. elegans adult animals. (cherrybiotech.com)

The authors propose that the asymmetric segregation of the NuRD complex in C. elegans is regulated in a V-ATPase-dependent manner, that this plays a crucial role in determining the differential expression of the apoptosis activator egl-1 and that it is therefore critical for the life/death fate decision in this species. (elifesciences.org)
Notably, although the phosphosites are not conserved between human and C. elegans nucleoporins, they are located in IDRs in both species. (ijm.fr)

In C. elegans, cogc-8 is involved in gonad morphogenesis and regulation of cell migration. (wormbase.org)

Fast Red) localized within hepatocytes of the liver tissue of an elegant-crested tinamou embryo by in situ hybridization ( Technical Appendix ). (cdc.gov)

Here we use Caenorhabditis elegans to study the role of centrosomes in setting spindle length. (princeton.edu)

Asymmetric segregation of NuRD during ACDs of C. elegans Q neuroblast. (elifesciences.org)

The first time I looked into the microscope to watch the embryo divide asymmetrically with such purpose, I knew I was hooked. (ursinus.edu)
Mesoscopic imaging of live or cleared samples of multiple biological models, like 3D organoids, fish (Danio) and mammalian embryos, can be observed by SPIM, OPT, SPIM-OPT and micro-CT, including multiple Zeiss Z.1 light-sheet systems, the ultra-microscope and home-built SPIM-OPT for cm-large samples. (eurobioimaging.eu)
The ZEISS Lattice Lightsheet 7 microscope enabled imaging of the entire depth of the embryos with little to no phototoxicity, at the required speed to prevent any motion artefacts, and with minimal loss of signal intensity. (zeiss.com)

This generally involves the action of cytoplasmic determinants, located within parts of the fertilized egg, and of inductive signals emitted from signaling centers in the embryo. (wikipedia.org)
In terms of the pattern of structures, this is controlled by a re-activation of signals active in the embryo. (wikipedia.org)

For time-lapse observation of early-stage D rosophila embryos, Keller et al. (nature.com)
The embryos at this stage are sensitive to changes in growth environment, structural integrity and phototoxicity. (zeiss.com)

Anatomy41

Organisms16

Diseases2

Chemicals and Drugs42

Analytical, Diagnostic and Therapeutic Techniques and Equipment33

Psychiatry and Psychology1

Phenomena and Processes63

Disciplines and Occupations4

Technology, Industry, Agriculture2

Information Science7

Health Care1

Mouse embryos5

Drosophila4

Zebrafish1

Polarity4

Embryonic2

Developmental8

Nematode6

Proteins5

Mitotic3

Dynamics2

Genes3

Endoderm2

Genetics1

Oocytes2

Development9

Posterior3

Zygotic1

Confocal1

Cells9

Biology1

Interactions1

Cellular1

Genome1

20181

Model4

Experiments5

Apoptotic1

Adult2

Species2

Cell1

Appendix1

Centrosomes1

Asymmetric1

Microscope3

Signals2

Stage2