• Our study suggests Myr insertion is involved in the sorting of membrane lipids around the protein binding site to prepare it for viral assembly. (elifesciences.org)
  • Our realistic membrane models confirm interactions with PIP 2 and PS lipids are highly favored around the HBR, and are strong enough to keep the protein bound even without Myr insertion. (elifesciences.org)
  • To obtain information about BIN1's interaction with themembrane in near native environments, we used artificial lipid systems suchas liposomes and lipids nanotubes.First, we have shown that electrostatic interactions are more important forBIN1 when binding to membranes with low curvature. (kth.se)
  • The formation ofinvaginations (or tubules) is regulated by the composition of negativecharged lipids in membrane bilayer or electrostatic residues on the BARdomain. (kth.se)
  • Lipofuscin consists of highly autofluorescent granules of oxidized proteins and lipids that build up in the lysosomes of aging cells in a variety of tissues. (biotium.com)
  • Most of the dystrophin protein consists of a central domain made of 24 spectrin-like coiled-coil repeats (R). Using small angle neutron scattering (SANS) and the contrast variation technique, we specifically probed the structure of the three first consecutive repeats 1-3 (R1-3), a part of dystrophin known to physiologically interact with membrane lipids. (cea.fr)
  • From these studies, we report an all-atom model of R1-3 that highlights the opening of the R1 coiled-coil repeat when bound to the membrane lipids. (cea.fr)
  • However, when these proteins are separated from lipids they often tend to aggregate and/or precipitate. (rcsb.org)
  • We aim to address this challenge from an innovative rheological perspective: we utilize hydrodynamic interactions to perturb and measure forces and microstructures in ILs mixed with key biological components such as proteins, lipids and water. (mit.edu)
  • Structure, function and organization of lipids and proteins in biological membranes. (biomembranes.nl)
  • Biogenesis of membranes and the transport and sorting of lipids and proteins. (biomembranes.nl)
  • The adsorption free energy of charged proteins on mixed membranes, containing varying amounts of (oppositely) charged lipids, is calculated based on a mean-field free energy expression that accounts explicitly for the ability of the lipids to demix locally, and for lateral interactions between the adsorbed proteins. (huji.ac.il)
  • The proteins are modeled as uniformly charged spheres and the (bare) membrane as an ideal two-dimensional binary mixture of charged and neutral lipids. (huji.ac.il)
  • the lipids, at a certain demixing entropy penalty, adjust their concentration in the vicinity of the adsorbed protein to achieve optimal charge matching. (huji.ac.il)
  • However, the molecular mechanisms by which lipids and membrane proteins accomplish these tasks are largely unknown. (virginia.edu)
  • This protein functions to regulate the levels of highly phosphorylated inositol lipids, such as PIP2, in the cell. (virginia.edu)
  • Unlike soluble globular proteins, membrane proteins have hydrophobic amino acid side chains exposed on their surfaces so that they can associate with and embed in hydrophobic phospholipid bilayers. (rcsb.org)
  • They can be covalently bound to lipid molecules or interact with the membrane via amphipathic alpha helices, hydrophobic loops, or electrostatic interactions. (rcsb.org)
  • 3D-QSAR is broadly used tool to recognize the steric, electrostatic, and hydrophobic interactions of various drugs acting via receptor modulation for exerting biological activity[ 14 , 15 ]. (ijpsonline.com)
  • A molecular model of the FMDV 3A protein, derived from the nuclear magnetic resonance (NMR) structure of the poliovirus 3A protein, predicted a hydrophobic interface spanning residues 25 to 44 as the main determinant for 3A dimerization. (asm.org)
  • Replacements L38E and L41E, involving charge acquisition at residues predicted to contribute to the hydrophobic interface, reduced the dimerization signal in the protein ligation assay and prevented the detection of dimer/multimer species in both transiently expressed 3A proteins and in synthetic peptides reproducing the N terminus of 3A. (asm.org)
  • In contrast to the signal sequences of exported proteins, the bacterial SRP signal sequences are more hydrophobic and are mostly "uncleaved signal sequences" present in membrane proteins that remain in the final protein-chain as transmembrane anchor sequences. (nature.com)
  • In general, the signal for insertion into the inner bacterial membrane is located in the first hydrophobic transmembrane domain and insertion is catalysed by the Sec translocase and/or YidC insertase. (nature.com)
  • Here, we show that KRas, but not HRas, acutely translocates from the plasma membrane (PM) to the Golgi complex and early/recycling endosomes in response to neuronal activity. (rupress.org)
  • Ras activity relies on proper anchoring to the cytoplasmic leaflet of the plasma membrane (PM) by means of a lipid-based PM targeting motif. (rupress.org)
  • The matrix (MA) domain drives Gag onto the plasma membrane through electrostatic interactions at its highly-basic-region (HBR), located near the myristoylated (Myr) N-terminus of the protein. (elifesciences.org)
  • Scaffolding proteins play important roles in supporting the plasma membrane (sarcolemma) of muscle cells. (cea.fr)
  • Direct measurement of the interaction of sucralose with biomolecules and lipid membranes will require both chemical and physical analysis. (experiment.com)
  • While various techniques have successfully reproduced these lipid membranes in the laboratory, their different modes of assembly produce artifacts in their structures. (mdpi.com)
  • The iron atoms are arranged in pairs in an iron-sulfur bridge, which is bounded to the sulfur atoms of Cysteine residues in the protein. (biochemden.com)
  • Replacements that favored (Q44R) or impaired (Q44D) the polar interactions predicted between residues Q44 and D32 did not abolish dimer formation of transiently expressed 3A, indicating that these interactions are not critical for 3A dimerization. (asm.org)
  • Lysine residues in the α-helical region of adipocyte and heart FABP had previously been identified as important for interactions between these proteins and the phospholipid interface. (soton.ac.uk)
  • The information within the KdpD protein that confers SRP interaction was found in the amino-terminal cytoplasmic domain of KdpD, particularly at residues 22-48. (nature.com)
  • When the KdpD peptides (residues 22-48) were fused to sfGFP the targeting to the membrane was observed by fluorescence microscopy. (nature.com)
  • We found that the amino acid residues R22, K24 and K26 are important for SRP interaction, whereas the residues G30, G34 and G36, essential for a functional Walker A motif, can be replaced with alanines without affecting the affinity to SRP-FtsY and membrane targeting. (nature.com)
  • Furthermore, it is suggested that the binding of SRP to the signal sequence is promoted by the presence of basic amino acid residues through electrostatic interactions 18 . (nature.com)
  • Adsorption of apolipoprotein A-I to biological membranes. (edpsciences.org)
  • Adsorption of a positively charged apo A-I macroion to the surface of the membrane modifies the electric field within the head group region and induces lateral demixing of phospholipid molecules in the membrane. (edpsciences.org)
  • and ii) cholesterol reduces macroion adsorption to the membrane by reducing the surface area of the membrane and restricting the dipoles range of rotation. (edpsciences.org)
  • Model predictions for the change in free energy of adsorption to zwitterionic membrane are in good agreement with previously reported experimental data with liposomes. (edpsciences.org)
  • Adsorption isotherms demonstrating the importance of lipid mobility and protein-protein interactions are calculated using an adsorption equation with a coverage-dependent binding constant. (huji.ac.il)
  • Finally, it is argued that the formation of lipid-protein domains may be enhanced by electrostatic adsorption of proteins, but its origin (e.g., elastic deformations associated with lipid demixing) is not purely electrostatic. (huji.ac.il)
  • Domain formation induced by the adsorption of charged proteins on mixed lipid membrane. (edu.ng)
  • Their unique hourglass-shaped architecture is highly conserved among different bacterial membrane proteins and other biological channels. (ibecbarcelona.eu)
  • Due to their large surface area and ability to interact with proteins and peptides, graphene oxides offer valuable physiochemical and biological features for biomedical applications and have been successfully employed for optimizing scaffold architectures for a wide range of organs, from the skin to cardiac tissue. (mdpi.com)
  • Sticholysins I and II, cytolysins purified from the sea anemone Stichodactyla helianthus, act by lysing biological and model membranes. (bvsalud.org)
  • Model membranes replicate biological membranes in a simplified lipid bilayer architecture, allowing for the study of the membrane's biophysical properties and interactions in a controlled environment. (mdpi.com)
  • SANS data for the protein/lipid complexes were obtained with contrast-matched bicelles under various phospholipid compositions to probe the role of electrostatic interactions. (cea.fr)
  • Membranes define cellular and organellar boundaries and are composed of phospholipid bilayers. (rcsb.org)
  • Membrane proteins are either embedded in or associated with the phospholipid bilayer. (rcsb.org)
  • Our model is based on the Haugen and May model which takes into account the dipolar nature of the zwitterionic phospholipid head group in the membrane, in which the positive end of the zwitterionic dipole moment can move randomly on a hemispherical surface with a radius equal to the arm of the dipole moment and with the negative end fixed at the hydrocarbon layer. (edpsciences.org)
  • In preliminary studies it was shown that LFABP released the fluorescent fatty acid 11(5-dimethylaminonapthalenesulphonylamino) undecanoic acid (DAUDA) in the presence of anionic phospholipid vesicles under conditions that indicated a direct interaction between the LFABP and the anionic phospholipid interface. (soton.ac.uk)
  • The ligand release that was observed with anionic vesicles was sensitive to the ionic strength of the assay conditions and the anionic charge density of the phospholipid at the interface, indicating that non-specific electrostatic interactions play an important role in the process. (soton.ac.uk)
  • The nature of this interaction involves both electrostatic and non-polar interactions as maximal release of LFABP from phospholipid vesicles with recovery of ligand binding cannot be achieved with high salt and requires the presence of a non-anionic detergent. (soton.ac.uk)
  • The results of these studies suggested that α-helix II, in particular, residue K31 of LFABP are involved in interactions with the anionic phospholipid interface. (soton.ac.uk)
  • Here, we present the BLaTM assay that measures homotypic as well as heterotypic TMD-TMD interactions in a bacterial membrane. (cipsm.de)
  • Hyperphosphorylated lipins are relatively unable to translocate onto endoplasmic reticulum membranes. (aocs.org)
  • When expressed as a recombinant protein in transfected cells, PV 3A cofractionates with endoplasmic reticulum markers ( 66 ), and its single transient expression can disrupt the secretory apparatus ( 23 ) and decrease major histocompatibility complex (MHC) class I expression ( 22 ). (asm.org)
  • Bacteria strictly regulate their cellular membrane composition in response to changes of environmental conditions, in order to adjust membrane fluidity and optimize associated membrane functions [ 14 ]. (biomedcentral.com)
  • Phosphorylation and dephosphorylation of proteins are mechanisms of activation and deactivation which regulate many cellular processes. (epj-nbp.org)
  • Here, we provide a comprehensive review of the molecular interactions that govern pH-dependent rearrangements and introduce a paradigm for electrostatic residue pairings that regulate progress through the viral fusion coordinate. (pacific.edu)
  • Functional reintegration into lipid environments represents a major challenge for in vitro investigation of integral membrane proteins (IMPs). (tudelft.nl)
  • The assembly of integral membrane protein complexes is frequently supported by transmembrane domain (TMD) interactions. (cipsm.de)
  • Finally, our strategy opens up new possibilities for structure determination of peripheral and integral membrane proteins not compatible with different high-resolution structural methods. (cea.fr)
  • The Cytochromes of type a and b and some of type "C" are integral proteins of the inner mitochondrial membrane. (biochemden.com)
  • Integral membrane proteins are permanently attached to a lipid bilayer, either embedded in or anchored to the membrane. (rcsb.org)
  • Peripheral membrane proteins form transient complexes with the membrane or with integral membrane proteins. (rcsb.org)
  • This automatic annotation pipeline identifies integral alpha-helical domains as well as beta barrels based on sequence features and then determines protein-lipid interactions using molecular dynamics simulations. (rcsb.org)
  • By contrast, MemProtMD specifically considers integral membrane proteins. (rcsb.org)
  • Despite having a small genome of its own, the vast majority of plastid-localized proteins is nuclear encoded and must be posttranslationally imported from the cytosol. (bvsalud.org)
  • The viral genome encodes four structural capsid proteins (VP1 to VP4) and seven nonstructural (NS) proteins, the leader Lb/ab protease, and proteins encoded in the P2 (2B and 2C) and P3 (3A, 3B, 3C, and 3D) regions ( 9 ). (asm.org)
  • Approximately 30 to 40 percent of the proteins in a genome are membrane proteins and 70 percent or more of current pharmaceuticals appear to act on membrane proteins. (virginia.edu)
  • Viral fusion proteins undergo dramatic conformational transitions during membrane fusion. (pacific.edu)
  • The most likely explanation for ligand release is that interaction of LFABF with an anionic membrane interface induces a rapid conformational change, reducing the affinity of DAUDA for the protein. (soton.ac.uk)
  • Using EPR spectroscopy in combination with site-directed mutagenesis and spin-labeling, we are probing the structures and conformational transitions in large membrane proteins that would normally be inaccessible using other structural methods. (virginia.edu)
  • Such a membrane-anchoring strategy has been adopted by a wide range of signaling proteins ( Fivaz and Meyer, 2003 ) with significant variations in the structure of these motifs (see Table S1). (rupress.org)
  • Thisimplies that BIN1 rather bundles actin than decorates single filaments.Third, we explored a strategy to purify an aggregation prone BAR protein.Aggregation is a property common in Peripheral Membrane Proteins. (kth.se)
  • For example, OPM covers a substantially higher fraction of peripheral membrane proteins than are represented in other resources. (rcsb.org)
  • When bound to anionic bicelles, large modifications of the protein threedimensional structure were detected, as revealed by a significant increase of the protein gyration radius from 42 5 1 to 60 5 4 A . R1-3/anionic bicelle complexes were further analyzed by coarse-grained molecular dynamics simulations. (cea.fr)
  • We reveal the formation of amorphous complexes via an en masse pathway and their relaxation into virions via a synchronous pathway. (cea.fr)
  • Our hypothesis is that this effect is of electrostatic origin and that the observed behavior is due to proton charge fluctuations of the histidines, promoting attractive electrostatic interactions between the positively charged proteins and the anionic surfaces, with concomitant counterion release. (lu.se)
  • In addition to parameters such as size and shape, chemical functionalities on the surface seem to play a critical role in binding to cell membrane and subsequent cellular uptake [2] and [3] . (materialstoday.com)
  • Regarding cell membrane, surface charge and membrane potential are often confused and a sharper distinction should be done between the two as they refer to different concepts. (materialstoday.com)
  • This is why cell membrane is generally considered as globally negatively charged [5] . (materialstoday.com)
  • The uptake of nanoparticles by cells can be viewed as a two-step process: binding to the cell membrane and internalization. (materialstoday.com)
  • The accepted explanation lies in the fact that electrostatic interactions are favored with cell membrane that is negatively charged. (materialstoday.com)
  • Consequently, the widespread theory that the preferential cellular uptake of positively charged nanoparticles over neutral or negatively charged nanoparticles is due to favorable electrostatic interactions with cell membrane seems simplistic and reductive. (materialstoday.com)
  • Different aspects of the interaction between peptides from the toxins' N-termini (StI1-31 and StI12-31 SELAGTIIDGASLTFEVLDKVLGELGKVSRK, and StII1-30 and StII11-30 ALAGTIIAGASLTFQVLDKVLEELGKVSRK) and model membranes - micelles and bilayers - have been studied to contribute to the elucidation of the toxins mechanism of action at the molecular level. (bvsalud.org)
  • The use of peptides was based on the hypothesis that potein fragments can eventually mimic the structure and activity of the whole protein. (bvsalud.org)
  • A crucial contribution to the ligand -receptor binding affinity is, in addition to their electrostatic and van der Waals interactions, the desolvation of the ligand . (rsc.org)
  • Free energy perturbation calculations have shown that the significant decrease of the penalty of desolvation, facilitating the entrance of the ligands into the binding site crevice, compensates for the weaker ligand -receptor interaction. (rsc.org)
  • For example, learning which parts of the protein face the cytosol and which parts are extracellular can help us recognize the ligand-binding and signaling domains of a membrane receptor. (rcsb.org)
  • The protein is targeted to the inner membrane surface and is released after contacting the SRP receptor protein FtsY. (nature.com)
  • Lipocalin 10 is essential for protection against inflammation-triggered vascular leakage by activating LDL receptor-related protein 2-slingshot homologue 1 signalling pathway. (uc.edu)
  • Each of these resources uses a variety of measures and strategies for annotating membrane proteins (reviewed in Shimuzu, 2018 ). (rcsb.org)
  • Here, we employed transmission electronmicroscopy and other biophysical methods to elucidate how BAR domainproteins steer processes at the membrane.In this work we studied the BAR protein bridging integrator 1 (BIN1), whichhas an established role in cancer, Alzheimer's disease and skeletalmyopathies. (kth.se)
  • These three processes (membrane binding, translocation, dephosphorylation) give rise to a cyclic dynamics known as the myristoyl-electrostatic switch. (epj-nbp.org)
  • Membranes and membrane proteins participate in some of the most important and interesting cellular processes. (virginia.edu)
  • Energy transduction, cell signaling, membrane excitability, secretion and immune recognition are examples of a few of the processes mediated by membrane proteins. (virginia.edu)
  • However, recent improvements in experimental design ( e.g. , use of cryo-electron microscopy and inclusion of detergents, lipid molecules, vesicles, and nanodiscs) provide a wealth of new possibilities for membrane protein structure determination. (rcsb.org)
  • Digitization of membrane adsorbers replaces the need for pH shifts or/and high salt concentrations without affecting the molecular structure of the target molecules. (i3membrane.de)
  • Our work will provide data on sucralose's impact on cellular membrane structure and its electrostatic interactions with proteins. (experiment.com)
  • Despite the importance of the PAP reaction, identification of the genes responsible for encoding PAP enzymes remained elusive until 2006 when the yeast PAP was identified and was shown to be an orthologue of a family of three mammalian proteins called lipins [reviewed in 1]. (aocs.org)
  • Cyt.c of mitochondria, a soluble protein that associates through electrostatic interactions with the outer surface of the inner membrane. (biochemden.com)
  • Although membrane proteins are composed of helices and sheets, they have some unique features and properties that distinguish them from any other soluble protein. (rcsb.org)
  • These proteins contain a very tightly, sometimes covalently bound flavin nucleotide, either FMN (or) FAD. (biochemden.com)
  • SRP is bound to the ribosome and is ready to interact with a nascent protein chain. (nature.com)
  • The amino-terminal NG domain of SRP is bound to the ribosomal proteins uL23 and uL29, next to the tunnel exit and the carboxy-terminal M domain to the ribosomal 23S RNA 6 . (nature.com)
  • Antibodies (Trastuzumab) bound at a Protein A membrane could be gained from the membrane by more than 95% using a certain voltage without shifting the pH-value. (i3membrane.de)
  • In addition to ion exchange membranes, affinity membranes allow specific binding of an analyte of interest through ligands, such as protein A. This allows analytes, such as monoclonal antibodies, to be purified. (i3membrane.de)
  • The polybasic nuclear localization sequence (NLS) on the lipins as well as the oligomerization of lipins mediates membrane association. (aocs.org)
  • Monotopic membrane proteins are attached to a single side of the lipid bilayer. (rcsb.org)
  • PDB structures of these proteins often do not include a lipid bilayer, so the membrane-binding regions have to be manually and/or programmatically annotated by experts. (rcsb.org)
  • An earlier study showed that, upon interaction with a common model bilayer, a protein cushion spontaneously forms underneath the bilayer. (lu.se)
  • The microstructures we probe can then be linked to critical events determining the performance of ILs, such as protein aggregation and membrane lipid extraction. (mit.edu)
  • Translocation is reversible and mediated by the polybasic-prenyl membrane targeting motif of KRas. (rupress.org)
  • We provide evidence that KRas translocation occurs through sequestration of the polybasic-prenyl motif by Ca 2+ /calmodulin (Ca 2+ /CaM) and subsequent release of KRas from the PM, in a process reminiscent of GDP dissociation inhibitor-mediated membrane recycling of Rab and Rho GTPases. (rupress.org)
  • General porins are nature's sieving machinery in the outer membrane of Gram-negative bacteria. (ibecbarcelona.eu)
  • For example, in gram negative bacteria, such as E. coli, we are examining the molecular mechanisms by which vitamin B-12 and iron are transported across the outer membrane. (virginia.edu)
  • The outer membrane transport proteins in E. coli are beta-barrel structures, which function to drive the accumulation of iron and vitamin B-12. (virginia.edu)
  • Poly-L-lysine (PLL) of extracellular matrix proteins is a small natural homopolymer of the essential amino acid L-lysine that is used to coat culture substrates. (hindawi.com)
  • KdpD is a four-spanning membrane protein that has two large cytoplasmic domains at the amino- and at the carboxyterminus, respectively. (nature.com)
  • Over the past decades, the atomistic simulation of membrane proteins has reached biologically relevant timescales and revealed important insights into their energetics and functional mechanisms. (stonybrook.edu)
  • One area of investigation involves studies on the mechanisms by which proteins become attached to membrane surfaces.This attachment is critical for cell-signaling because it controls protein-protein interactions and the access of enzymes to lipid substrates. (virginia.edu)
  • However, the biomolecules are recovered by pH shifts or/and high salt concentrations from ion exchange membranes or affinity membranes, which minimizes the yield and maximizes the cost of the process. (i3membrane.de)
  • The digital desorption works with AEX Membrane (use of BSA protein) as well as with affinity chromatographic membranes. (i3membrane.de)
  • Membrane proteins are crucial for cell survival and communication across membranes, serving as molecular transporters , signal receptors , ion channels , and enzymes . (rcsb.org)
  • Majority of research lacks a high-resolution structure of the target protein i.e. membrane spanning G-proteincoupled receptors and membrane transporters. (ijpsonline.com)
  • Cell membranes possess strong electrochemical gradients, which form the basis of membrane voltage and drive the function of transporters and ion channels. (stonybrook.edu)
  • We present a molecular dynamics simulation study on the binding mechanism of the membrane targeting domain of HIV-1 Gag protein. (elifesciences.org)
  • Do molecular interactions with sucralose alter gut bacteria and cause glucose intolerance? (experiment.com)
  • The membranes that define these organelles require complex protein sorting and molecular machines that selectively mediate the import of proteins from the cytosol to their functional location inside the organelle. (bvsalud.org)
  • These proteins are encoded as a larger molecular weight precursor that contains a special "zip code," a targeting sequence specific to the intended final destination of a given protein. (bvsalud.org)
  • The role of biomembranes in signal transduction via membrane receptors, cell-cell-, and cell-matrix interactions. (biomembranes.nl)
  • For example, the activation of G-protein coupled receptors has been reported to be voltage-sensitive. (stonybrook.edu)
  • This review critically focuses on opportunities to employ protein-graphene oxide structures either as nanocomposites or as biocomplexes and highlights the effects of carbonaceous nanostructures on protein conformation and structural stability for applications in tissue engineering and regenerative medicine. (mdpi.com)
  • For these reasons, membrane proteins represent one of the most important challenges in the field of structural biology. (virginia.edu)
  • They represent one of only a few classes of transport proteins for which accurate high-resolution structural models have been obtained. (virginia.edu)
  • We are currently studying the structure and electrostatic interactions made by MARCKS (the myristoylated alanine rich C-kinase substrate), which has a highly positively charged domain that attaches to the membrane interface. (virginia.edu)
  • NS proteins are involved in crucial aspects of the viral cycle and pathogenesis, such as rearrangements of intracellular membranes required for endomembrane recruitment and the lysis of host cells ( 1 , 12 , 14 , 18 , 73 ). (asm.org)
  • in poliovirus (PV), the interaction between the RNA replication complex and intracellular membranes appears to be accomplished by proteins 3A and 2C, which have membrane-binding properties ( 11 , 60 ). (asm.org)
  • On the other hand, 3AB presumably anchors 3B in intracellular membranes originated de novo during the early steps of RNA replication, where uridylylated 3B primes the synthesis of nascent viral RNAs ( 2 , 37 , 68 , 69 ). (asm.org)
  • Liver fatty acid binding protein (LFABP) belongs to a family of small (14kDa) intracellular lipid-binding proteins, which have a characteristic β-barrel structure. (soton.ac.uk)
  • Since the membrane-associated and membrane-traversing regions of membrane proteins have distinct properties, recognizing these regions in the protein can help us understand the stability and functions of membrane proteins. (rcsb.org)
  • Our goal is to understand how sucralose interacts with biomolecules so we can isolate its effect on the digestive system and understand its interactions in the natural environment. (experiment.com)
  • Membrane chromatography is considered a promising method for the purification and separation of diverse biomolecules such as proteins and antibodies. (i3membrane.de)
  • Various biomolecules with a different charge, but similar molecule weight, can be easily separated as a result of electrostatic interactions between the surface charge of the ion-exchange membrane and the charged groups. (i3membrane.de)
  • By functionalization of the ion-exchange membrane with a nanometer-sized layer of gold, the chemical binding capacity of biomolecules can be switched when it is connected to an electrical source. (i3membrane.de)
  • In the present work, we use a wide range of techniques (SANS, SAXD, DSC, ITC, CD, and confocal and electron microscopy) in order to fully characterize the interaction of a cecropin A-melittin hybrid antimicrobial peptide, CA(1-7)M(2-9), of known antimicrobial activity, with a bacterial model membrane of POPE/POPG in an effort to unravel its mechanism of action. (cea.fr)
  • Experiments with model membrane systems have indicated, however, that membrane cholesterol reduces apo A-I binding to the membrane. (edpsciences.org)
  • The physiological role of FABP's within the cell remains unclear but a targeting role for the protein would provide an attractive mechanism for ligand (fatty acid) transfer to membrane sites for further metabolism and requires an interaction between the FABP and the membrane. (soton.ac.uk)
  • As a result, experimentally determined structures of membrane proteins are underrepresented in the PDB archive. (rcsb.org)
  • How are membrane protein structures identified in the PDB? (rcsb.org)
  • Some membrane protein structures are unanimously annotated by all four external resources. (rcsb.org)
  • Depending on the annotation strategy used, some membrane protein structures are only annotated by a single resource. (rcsb.org)
  • 20. Sgourakis NG, Lange OF, DiMaio F, André I, Fitzkee NC, Rossi P, Montelione GT, Bax A, Baker D. Determination of the structures of symmetric protein oligomers from NMR chemical shifts and residual dipolar couplings. (lu.se)
  • C2 domains function attach to membranes in a Ca++ dependent fashion, and PH domains translocate to the membrane interface and bind to PIP2. (virginia.edu)
  • MARCKS is a protein which binds to the membrane by electrostatic interaction. (epj-nbp.org)
  • During its biogenesis KdpD binds to the signal recognition particle (SRP) of Escherichia coli that consists of a 48-kDa protein Ffh and a 4.5S RNA. (nature.com)
  • SRP is universally conserved in its core region that consists of a ribonucleoprotein particle, the SRP RNA (4.5S RNA in E. coli ) and the protein component SRP54 (Ffh in E. coli for "fifty-four-homolog") that binds to the conserved RNA domain IV 4 . (nature.com)
  • This resource is based on the TMDET algorithm ( Tusnády, 2004 ) that detects membrane proteins by their 3D structure. (rcsb.org)
  • The commonly used dip-and-read test strip (dipstick) mainly detects albumin among the various proteins in urine. (medscape.com)
  • Microtubule-associated protein MAP1LC3C regulates lysosomal exocytosis and induces zinc reprogramming in renal cancer cells. (uc.edu)
  • The functional relevance of this diversity in membrane anchors remains largely unexplored. (rupress.org)
  • Minimization of this free energy functional yields the familiar nonlinear Poisson-Boltzmann equation and the boundary condition at the membrane surface that allows for lipid charge rearrangement. (huji.ac.il)
  • second, three maize embryo LEA proteins from groups 1, 2, and 3 are analyzed in order to detect functional differences among them and finally, transgenic maize plants over-expressing group 5 "rab28" lea gene are characterized. (ndltd.org)
  • At high curvature,binding is likely driven by non-polar interactions. (kth.se)
  • To characterize this behavior, we identify two distinct residue pairings, which we define as Histidine-Cation (HisCat) and Anion-Anion (AniAni) interactions. (pacific.edu)
  • The overview shows two membrane patches facing the cantilever with different sides: left the extracellular side and right the cytoplasmic side. (nanosurf.net)
  • Insertion events only occur with one of the membrane models, showing a combination of surface charge and internal membrane structure modulate this process. (elifesciences.org)
  • The application of EPR spectroscopy to membrane protein structure is relatively recent. (virginia.edu)
  • 26. Rämisch S, Lizatovic and Andre I. "Exploring alternate states and oligomerization preferences of coiled-coils by de novo structure modeling" Proteins, 2014. (lu.se)
  • Benzylamine derivatives have recently been demonstrated interesting potential as the treatment for chronic obstructive pulmonary disease via interaction with the PDE4 enzymes. (ijpsonline.com)
  • Non-specific fluorescence background can have several causes, including sample autofluorescence, non-specific antibody binding, and electrostatic interactions of fluorescent dyes with proteins and blotting membranes. (biotium.com)
  • In this work, FMDV 3A homodimerization was evidenced by an in situ protein fluorescent ligation assay. (asm.org)
  • It is translocated from the membrane and phosphorylated by Protein Kinase C. Back in the cytoplasm the translocated MARCKS proteins are dephosphorylated by the enzyme phosphatase and can reattach to the membrane. (epj-nbp.org)
  • Herein, we start from a reaction-diffusion model taking into account mass conservation of the MARCKS proteins. (epj-nbp.org)
  • Furthermore, we show that the model fits previous experimental results well and predicts, in addition, the formation of domains with high concentration of MARCKS proteins at the membrane. (epj-nbp.org)
  • We have developed a simple model of binding, phosphorylation and desphosphorylation for MARCKS protein. (epj-nbp.org)
  • Here, we describe further development of an in vivo library selection platform based on self-assembling protein nanoparticles encapsulating their own mRNA genomes (synthetic nucleocapsids or synNCs). (uw.edu)
  • However, the caveat remains that quantitative estimates of proteinuria performed at clinical chemistry laboratories reflect the sum total of several classes of proteins and yield a result greater than the actual amount of albumin in the specimen. (medscape.com)
  • 29. Rämisch S, Lizatović R, André I. Automated de novo phasing and model building of coiled-coil proteins. (lu.se)
  • The investigation of nanoparticle/cell interactions is a crucial issue with regard to two fields: nanomedicine and nanotoxicology. (materialstoday.com)
  • A second area of investigation involves membrane transport. (virginia.edu)
  • We conclude that the sarcolemma membrane anchoring that occurs during the contraction/elongation process of muscles could be ensured by this coiled-coil opening. (cea.fr)