Electroporation. Medical search. Frequent questions

November 23, 2010 (New York City) - Irreversible electroporation (IRE) holds promise for the treatment of hepatocellar carcinoma (HCC) and other soft tissue tumors. (medscape.com)
Purpose To compare the accumulation of liposomal doxorubicin in liver tissue treated by radiofrequency ablation (RFA) and irreversible electroporation (IRE) in in vivo porcine models. (muni.cz)
We hypothesize this will allow simultaneous safe treatment of all nodules by irreversible electroporation (IRE) when applied in a transhepatic fashion. (upf.edu)
Irreversible electroporation blijkt succesvolle aanpak bij patiënten met inoperabele, onbehandelbare levertumoren. (kanker-actueel.nl)
Martijn Meyerink en arts-onderzoeker Hester Scheffer over de nanoknife - irreversible electroporation. (kanker-actueel.nl)
Irreversible electroporation of a hepatocellular carcinoma lesion adjacent to a transjugular intrahepatic portosystemic shunt stent graft is gratis in te zien. (kanker-actueel.nl)
Percutaneous irreversible electroporation (IRE) of hepatic malignant tumours: Contrast-enhanced ultrasound (CEUS) findings waarvan een presentatie werd gegeven april 2013 op het ARRS congres, zie ook onderstaand artikel. (kanker-actueel.nl)
Met de nanoknife - IRE - Irreversible electroporation bleek 70 procent van de tumoren volledig te zijn vernietigd. (kanker-actueel.nl)
IRE - Irreversible electroporation verstoort de celmembraan en leidt automatisch tot celdood. (kanker-actueel.nl)
Electroporación irreversible en el tratamiento de los cánceres de páncreas e hígado. (sergas.gal)
The irreversible electroporation (IRE) procedure The NanoKnife IRE procedure used in the Heidelberg Clinic for Prostate Therapy was developed by the American company AngioDynamics (New York, USA) and is being used by the Heidelberg urologists for the very first time in Germany as a standardised procedure for the treatment of prostate cancer. (prostata-therapie.de)
The breakdown potential of irreversible electroporation, the most critical parameter in electroporation process, can be detected by analyzing current signals as well. (asme.org)
Figure 2 illustrates a typical electrical signature in an irreversible electroporation process. (asme.org)
The use of irreversible electroporation (IRE) for cancer treatment has increased during the past decade due to many advantages over other focal therapies. (umn.edu)
Jiang, C , Shao, Q & Bischof, JC 2015, ' Pulse Timing During Irreversible Electroporation Achieves Enhanced Destruction in a Hindlimb Model of Cancer ', Annals of Biomedical Engineering , vol. 43, no. 4, pp. 887-895. (umn.edu)
Herein we aimed to ascertain whether recovery of neurosurgical high-frequency irreversible electroporation (HFIRE) injury in brain tissues can be accelerated by 7,8-dihydroxyflavone (7,8-DHF). (bvsalud.org)
Irreversible electroporation (IRE) for the management of unresectable pancreatic VIPoma has been reported. (medscape.com)
Treatment of breast cancer through the application of irreversible electroporation using a novel minimally invasive single needle electrode. (cocites.com)
Irreversible electroporation and nivolumab combined with intratumoral administration of a toll‐like receptor ligand, as a means of in vivo vaccination for metastatic pancreatic ductal adenocarcinoma (Panfire‐iii). (vumc.nl)
Irreversible Electroporation (IRE) is a novel image-guided tissue ablation technology that induces cell death via very short but strong pulsed electric fields. (upf.edu)

The electroporation technique involves exposure of cell membranes to high-intensity electric pulses that cause a temporary destabilization in certain areas of the cell. (transparencymarketresearch.com)
We observe that when electroporation is performed in media with lower ionic concentration than cells cytoplasm, their internal conductivity decreases after electroporation depending on the intensity of applied pulses. (umanitoba.ca)
Our results show that electroporation in very low conductivity media requires stronger pulses to achieve a similar poration extent as in high conductivity media. (umanitoba.ca)
Dermal Electroporation is an advanced, results-driven therapy that uses electric pulses to permeate into the deepest layers of the skin and split open skin cells. (artisanwellness.sg)
During electroporation, high electric pulses that last a few microseconds to milliseconds splits open skin cells around the treatment area and creates temporary pores. (artisanwellness.sg)
Electroporation makes use of electric pulses, creating gaps in the stratum corneum layer of the skin so that beauty enhancers and medications can be introduced into the basal and dermis layers of the skin. (skinandhairacademy.in)
electroporation beauty instrument, focus the import electroporation, needle-free mesotherapy, radiofrequency current RF, microwave pulses, and IPL Acne remove five major tip technologies, lets you synchronize solve multiple years problems, and glowing your skin's natural young color! (angelshealingtouch.com)

Our electroporation cuvettes are molded in high quality polycarbonate, with extremely precise and uniform electrode gaps. (eurogentec.com)

Unlike standard cuvette-based electroporation chambers, the Neon system uses a proprietary biologically compatible pipette tip chamber that generates a more uniform electric field. (thermofisher.com)
Standard electroporation cuvette compared to Neon pipette tip. (thermofisher.com)
Each sterilized Cuvettes Plus package includes a disposable cuvette and a transfer pipette, which is used for removal of the sample after electroporation. (llg.de)
8. Transfer the mixture to the electroporation cuvette (Bio-Rad, light blue cap). (ucsf.edu)
If you see the bubbles, then place your actual sample cuvette and perform electroporation. (ucsf.edu)

MaxCyte transient transfection is completed in three steps: cell harvesting, electroporation, and post electroporation cell usage. (cellculturedish.com)
Using the methods from PMC6656429 , and conditions from the manufacturer , I began with electroporating some HEK293 cells with GFP protein to identify conditions where I got the highest level of protein electroporation and sufficiently high viability post-electroporation. (openlabnotebooks.org)
I noted that the protein electroporation is most successful with higher levels of protein concentration and with post-electroporation treatment of cells with trypsin. (openlabnotebooks.org)

17 februari 2014: toegevoegd een zogeheten case report studie van een 65 jarige man man met onbehandelbare primaire levertumoren die naast een perfusie (lokale chemo) via een portocath systeem toch ook met de nanoknife kon worden geholpen en alsnog klinisch kankervrij werd. (kanker-actueel.nl)

Cite this: Electroporation Effective in Hepatocellular Carcinoma - Medscape - Nov 23, 2010. (medscape.com)

Electroporation, or electropermeabilization, is a microbiology technique in which an electrical field is applied to cells to boost permeability of the cell membrane. (skinandhairacademy.in)
With the ability to manipulate individual cells and detect the electrical potentials that induce electroporation in each cell, the chip can be used to study the fundamental biophysics of membrane electropermeabilization on the single cell level and in biotechnology, for controlled introduction of macromolecules, such as DNA fragments, into individual cells. (asme.org)
Membrane Electroporation and Electropermeabilization: Mechanisms and Models. (cocites.com)

The Neon Transfection System offers an innovative electroporation transfection method that utilizes a proprietary biologically compatible pipette tip chamber to generate a more uniform electric field for a significant increase in transfection efficiency and cell viability. (thermofisher.com)
In the poster, MaxCyte describes their flow electroporation transfection system that provides a cell type flexible and fully scalable method of transient gene expression. (cellculturedish.com)
MaxCyte has developed an efficient and scalable transfection system based on electroporation. (cellculturedish.com)

According to the report, the global electroporation instruments market was valued at ~US$ 126 Mn in 2019 and is projected to reach ~US$ 244 Mn by 2030, expanding at a CAGR of ~6% from 2020 to 2030. (transparencymarketresearch.com)

Andreason, G. & Evans, G. Induction and expression of DNA molecules in eukaryotic cells by electroporation. (nature.com)
Features of the electroporation technique, such as high specificity for targeting cancer cells and high degree for localization of treatment result in better treatment of internal tumors. (transparencymarketresearch.com)
The complete removal internal tumors includes positioning the electrode accurately to extend the electroporation in all tumor cells and to avoid damaging the healthy tissue or organs in the vicinity. (transparencymarketresearch.com)
Selective modification of antigen-specific T cells by RNA electroporation. (duke.edu)
It has been observed that the efficient transfection of T cells by RNA electroporation requires prior activation of T cells with mitogens or by anti-CD3 antibody stimulation. (duke.edu)
Using electroporation of mRNA encoding green fluorescent protein as a marker gene, we demonstrate that RNA electroporation can efficiently allow for the separation of cytomegalovirus-specific CD8+ and CD4+ T cells from bulk culture responding to cytomegalovirus pp65 antigen-pulsed dendritic cells. (duke.edu)
Electroporation affects the dielectric properties of cells. (umanitoba.ca)
In this thesis we introduce a dielectrophoresis (DEP) based technique to study changes in the cytoplasm conductivity of single Chinese hamster ovary (CHO) cells immediately after electroporation. (umanitoba.ca)
Using a microfluidic chip, we study changes in the DEP response of single CHO cells a few seconds after electroporation. (umanitoba.ca)
We also observe that with reversible electroporation there is a limit on the decrease in the cells' internal conductivity. (umanitoba.ca)
We monitor the temporal response of cells after electroporation to measure the time constant of changes due to ion transport and observe this ranges from seconds to tens of seconds depending on the applied pulse intensity. (umanitoba.ca)
Lastly, we measure the electroporation of cells in media with different conductivities. (umanitoba.ca)
An ultimate goal of this thesis was to establish proof-of-principle assays for delivery of various bioactive molecules into adherent cells by in situ electroporation, and to monitor how these compounds influence cellular behavior, once they are internalized within the cell cytosol. (uni-regensburg.de)
Studies of in situ electroporation (ISE) were conducted using different types of mammalian cells (BAEC, CHO-K1/CHO-GFP, HaCaT, NRK and NIH-3T3) grown to confluence on small planar gold film electrodes. (uni-regensburg.de)
Besides fluorescent DNA aptamers, various types of siRNA molecules were successfully delivered into cells by in situ electroporation and their long-term sequence-specific silencing effect on cells was demonstrated and quantified by using microscopy and/ or impedance analysis. (uni-regensburg.de)
In conclusion, this thesis demonstrated that in situ electroporation allows for highly efficient delivery of emerging types of molecules into monolayers of various types of cells. (uni-regensburg.de)
Electroporation derived from molecular biology makes it possible to induce substances into the heart of the cells. (magic-room16.com)
BTX Cuvettes Plus are designed for use in electroporation and electrofusion of bacteria, yeast, insect, plant and mammalian cells. (llg.de)
Electroporation import: special currents activate the skin cells, make the effective skincare cream ingredients directly into the dermal layer.10 times increase the skin absorption! (angelshealingtouch.com)
2.Electroporation import: through special current open the gap of the skin cells, making the effective skincare cream ingredients directly into the dermal layer.10 times increase absorption through the skin! (angelshealingtouch.com)
The biotech company's electroporation device is designed to locally deliver DNA-based interleukin-12 to stimulate the immune system and fight off cancer cells. (drugdeliverybusiness.com)
Mesenchymal stem cells modified by the fusion gene iRGD-Lamp2b were constructed to separate and purify exosomes, and the anti-miRNA-221 oligonucleotide (AMO) was loaded into the exosomes by electroporation. (frontiersin.org)
Since the first report on gene transfer by electroporation 1 , it has become a standard method for introduction of macromolecules into cells 2 3 4 . (asme.org)
Currently, electroporation is normally done in batches of cells between electrodes and there is little control over the permeabilization of individual cells. (asme.org)
Therefore, it is very difficult to study the fundamental biophysics of cell membrane electro-permeabilization and to design optimal electroporation protocols for individual cells 2 3 . (asme.org)
From this idea, we have developed a new micro-electroporation technology that employs a "bionic" chip to study and control the electroporation process in individual cells. (asme.org)
Electroporation involves the application of an electric field to cell suspensions, causing the cell membrane to become transiently permeable, which then encourages the entry of external materials into the cells. (cellculturedish.com)
By utilizing the principles of electroporation, MaxCyte has developed a system that permits transfection of a wide range of cells with DNA, RNA, proteins or cell lysates and has demonstrated typical transfection efficiencies and cell viabilities at greater than 90% even for more difficult to transfect cells such as CHO cells. (cellculturedish.com)
Another advantage of using electroporation is that it does not require specialized constructs, engineered cells, media additives or chemical reagents. (cellculturedish.com)
This scalable system is able to transfect from 5×10 5 cells in seconds using small-scale, static electroporation to 2×10 11 cells in less than 30 minutes by using flow electroporation. (cellculturedish.com)
Relative titers far all three sets of cells measured two weeks post EP demonstrate reproducibility and scalability of MaxCyte electroporation. (cellculturedish.com)
Using MaxCyte's flow electroporation system, CHO cells can produce secreted antibody titers over 2.7 g/L with optimization of post transfection culture conditions. (cellculturedish.com)
Transformation of Bacillus cereus vegetative cells by electroporation. (cocites.com)
Therefore, to enable the screening of as many genes as possible in primary cells, this protocol has reduced the cell number and reagent required per gene electroporation, whilst ensuring the maintenance of CRISPR editing efficiency. (slas-discovery.org)
These results were disheartening so I began exploring alternative means of expressing HTT in cells, including protein transfection and protein electroporation. (openlabnotebooks.org)
Electroporation has also been reported to successfully deliver proteins into cells. (openlabnotebooks.org)
GFP protein electroporation in HEK293 cells. (openlabnotebooks.org)

High-efficiency in vivo gene transfer using intraarterial plasmid DNA injection following in vivo electroporation. (nature.com)
This protocol describes the ex ovo electroporation of a DNA plasmid into avian embryos as young as stage X, nearly 24 h earlier in development than most electroporation protocols. (zenodo.org)
n\n6.Prepare the plasmid DNA for electroporation by mixing a 5 \u03bcg/\u03bcL concentrated stock of endotoxin-free plasmid with buffered phenol red solution in a 1:1 ratio, such that the final DNA concentration is ~2.5 \u03bcg/\u03bcL. (zenodo.org)
Plasmid electroporation of Sinorhizobium strains: The role of the restriction gene hsdR in type strain Rm1021. (cocites.com)
High-voltage electroporation of bacteria: genetic transformation of Campylobacter jejuni with plasmid DNA. (cocites.com)

In terms of product, the global electroporation instruments market has been segmented into total electroporation system, eukaryotic electroporation system, and microbial electroporation system. (transparencymarketresearch.com)

LED phototherapy: repair and heal the mirco gaps of the electroporation, needle-free mesotherapy ultra- fine skin, the skin pores and has calming anti-inflammatory effect. (angelshealingtouch.com)
5. LED phototherapy: LED phototherapy can effectively repair and healing the mirco gaps of the electroporation, needle-free mesotherapy ultra- fine skin, the skin pores and then import the wound period, calming anti-inflammatory effect. (angelshealingtouch.com)

What are the benefits of Dermal Electroporation? (artisanwellness.sg)
Dermal Electroporation is used to treat dry skin and is commonly used with substances that have healing, balancing and revitalising qualities. (artisanwellness.sg)
Patients who undergo dermal electroporation can anticipate magnified results like an increase in collagen production and younger looking and rejuvenated skin. (artisanwellness.sg)
What products are used with Dermal Electroporation? (artisanwellness.sg)
When combined with the best products, Dermal Electroporation ensures patients get healthy, glowing, vibrant and rejuvenated skin! (artisanwellness.sg)
Is Dermal Electroporation painful? (artisanwellness.sg)
Dermal Electroporation generally has no side effects, but if the device is not optimised properly with the right amount of electrodes, it could result in unwanted side effects like itching and a pricking sensation. (artisanwellness.sg)
Is Dermal Electroporation suitable for everyone? (artisanwellness.sg)

High efficiency transformation by electroporation of Pichia pastoris pretreated with lithium acetate and dithiothreitol. (cocites.com)

Electroporation is discussed as a new technique in the VIDEO Current State of Atrial Fibrillation Ablation Technologies . (dicardiology.com)

2008) A novel electroporation method using a capillary and wire-type electrode. (thermofisher.com)

A cross-section of the glass-bottomed electroporation chamber used to electroporate pre-gastrulation stage avian embryos. (zenodo.org)

North America dominated the global electroporation instruments market in 2019, followed by Europe. (transparencymarketresearch.com)

This approach identifies toxHPUF-encoding genes through comparison of gene-specific read coverages in DNA from pooled ligation mixtures before electroporation and pooled transformants after electroporation. (helsinki.fi)

Microalgae amino acid extraction and analysis at nanomolar level using electroporation and capillary electrophoresis with laser-induced fluorescence detection. (cocites.com)

Reversible electroporation, a technique that has been available for some time, uses short-pulse, high-voltage direct current to increase cell membrane permeability by disrupting its integrity, allowing transportation of drugs or gene therapy into the cell. (medscape.com)

It occurred to us that if electroporation induces pores in the cell membrane, then in a state of electroporation, a measurable current should flow through the individual cell. (asme.org)
Application of PFA for PVI, as described by Verma, creates the lesions by exposing the myocardial targets to high-gradient electric fields, leading to a nonthermal electroporation process that rapidly renders myocyte membranes "hyperpermeable," which induces apoptotic cell death. (medscape.com)

MaxCyte instruments are supplied with pre-loaded electroporation protocols optimized for a wide range of cell types (Figure 1), thereby simplifying assay development and maximizing performance and reproducibility. (cellculturedish.com)

Technological advancements have led to the efficient use of electroporation in the treatment of internal tumor. (transparencymarketresearch.com)
Advancements in the electroporation technology present better treatment option of liver and bone metastases, brain tumors, soft tissue sarcomas, and colorectal and esophageal tumors. (transparencymarketresearch.com)
ONCS ) said today that it won Fast Track Designation from the FDA for its ImmunoPulse IL-12 electroporation gene therapy for the treatment of metastatic melanoma. (drugdeliverybusiness.com)

Efficient gene transfer by electroporation of chick embryos in ovo has allowed the development of new approaches to the analysis of gene regulation, function and expression, creating an exciting opportunity to build upon the classical manipulative advantages of the chick embryonic system. (nature.com)
Figure 1: Electroporation-mediated gene expression in cultured chick and mouse embryos. (nature.com)
Thus, positioning of the specimen and consistency of the electroporation site between embryos is improved. (zenodo.org)

Impedance monitoring of in situ electroporation conducted with high time resolution showed biphasic changes of impedance signal after pulse application, indicating (i) fast recovery of the cell membrane integrity and (ii) relatively slow process of cell recovery after changes induced by membrane permeabilization. (uni-regensburg.de)
Although the biophysics of electroporation are still not fully understood, indirect evidence shows that micro aqueous pores with diameters of tens to hundreds of angstroms are created in the cell membrane due to the electrical field induced structural rearrangement of the lipid bilayer 5 . (asme.org)
However, when the electrical potential across the electrodes is sufficient to induce electroporation, a measurable current will flow through the pores of the cell membrane and between the electrodes. (asme.org)
Once the target cell is electroporated by the application of sufficient electroporation electrical potentials, macromolecules that are normally impermeant to cell membrane can be uploaded into the cell. (asme.org)
IMSEAR at SEARO: Electroporation of red blood cell membrane and its use as a drug carrier system. (who.int)

High processing volume can be attributed to patented electroporation chamber configuration combined with proprietary electrofusion medium. (cytopulse.com)

The electroporation process, or electro-permeability, reduces the cell wall's resistance and enables it to be more permeable, thus allowing nutrients to penetrate deeply into the skin. (artisanwellness.sg)
Also, electroporation function lets solutions penetrate deeply into the skin with significantly more product penetration that Sonophoresis and Iontophoresis. (alisa.shop)

The segment is expected to expand significantly over the next few years due to increase in applications of electroporation in microbiology, oncology, surgery, biochemistry, public health, and other non-communicable diseases. (transparencymarketresearch.com)

Figure 2: Electroporation into different chick tissues. (nature.com)

Gene transfer into human leukemia cell lines by electroporation: experience with exponentially decaying and square wave pulse. (nature.com)

Hence, it is responsible to ensure the electroporation device is used correctly. (artisanwellness.sg)
D-cool is a multi-functional device with Heating, Cooling and Electroporation functions. (alisa.shop)

Unlike micro needling , electroporation is a non-invasive and painless alternative. (skinandhairacademy.in)
Electroporation is a painless, non-invasive therapy for better skin with no side effects. (skinandhairacademy.in)

One poster that I thought provided a scalable and highly efficient system for transient protein expression was presented by MaxCyte, "Stable Antibody Production from CHO Cell Line of Choice Using Flow Electroporation. (cellculturedish.com)

The pores are not permanent and will close after locking in vital nutrients, or once the electroporation machine is shut down. (artisanwellness.sg)
PEF energy ablates non-thermally by creating microscopic pores in cell membranes, called electroporation. (dicardiology.com)

Describe different opsin delivery methods, such as virus injection, electroporation, and use of transgenic mice. (sfn.org)

In addition to these treatments, electroporation can also be used to take care of breast firming, keloid scars, cellulitis, stretch marks, flaccidity and more. (skinandhairacademy.in)

In addition, high demand for biopharmaceuticals is anticipated to boost the growth of the electroporation instruments market as well. (prnewswire.com)
This study identifies high demand for biopharmaceuticals as the prime reasons driving the electroporation instruments market growth during the next few years. (prnewswire.com)
This design allows a better maintenance of physiological conditions resulting in very high cell survival compared to conventional electroporation. (thermofisher.com)
Electroporation is an easy, non-chemical technique that yields high transformation efficiencies in various cell types. (transparencymarketresearch.com)
Identical electroporation parameters and cell handling are used for small and large-scale electroporation enabling streamlined scale up to high throughput transfection. (cellculturedish.com)
Recovery of neurosurgical high-frequency electroporation injury in the canine brain can be accelerated by 7,8-dihydroxyflavone. (bvsalud.org)

The method described here uses relatively low voltage, and the electroporation chamber can be made easily, with no specialized equipment required. (zenodo.org)
The chamber is constructed such that the raised segment of the anode is bathed in electrolyte solution (HBSS) during electroporation. (zenodo.org)

Dr. Fan Yuan gave a Plenary Lecture on mechanisms of cell electrotransfection at the 2nd World Congress on Electroporation and Pulsed Electric Fields in Biology, Medicine and Food & Environmental Technologies. (duke.edu)

Rise in demand for multifaceted devices that can be used for all types of cell studies leading to overall cost-effectiveness is anticipated to drive the total electroporation system during the forecast period. (transparencymarketresearch.com)
The Cellaxess electroporation system, which can be used for the in-situ transfection of any adherent cell-type, ensures minimal cell processing for perfectly retained cell function and morphology. (bio-equip.cn)
In the poster, " Stable Antibody Production from CHO Cell Line of Choice Using Flow Electroporation, " MaxCyte describes their system and presents data to demonstrate the advantages of the flow electroporation approach. (cellculturedish.com)

Electroporation of short hairpin RNAs for rapid and efficient gene knockdown in the starlet sea anemone, Nematostella vectensis. (stowers.org)

therefore, gene editing using CRISPR (clustered regularly interspaced short palindromic repeats)/Cas9 is often carried out through ribonucleoprotein (RNP) electroporation. (slas-discovery.org)

Induced transmembrane voltage and its correlation with electroporation-mediated molecular transport. (cocites.com)

Our reports on electroporation instruments market provides a holistic analysis, market size and forecast, trends, growth drivers, and challenges, as well as vendor analysis covering around 25 vendors. (prnewswire.com)

Can be used with electroporation systems from different manufacturers (not suitable for units from Eppendorf). (llg.de)

Figure 4: Application of electroporation to analysis of gene regulation. (nature.com)
The micro-electroporation chip, shown schematically in Figure 1, is designed and fabricated using standard silicon microfabrication technology. (asme.org)
Figure 3 shows how a cell entrapped in a hole is loaded during electroporation with a fluorescent die. (asme.org)

Based on application, the global electroporation instruments market has been classified into bio-medical research, therapeutic delivery, and protein production. (transparencymarketresearch.com)

Anatomy1

Organisms1

Chemicals and Drugs6

Analytical, Diagnostic and Therapeutic Techniques and Equipment10

Phenomena and Processes10

Irreversible20

Pulses7

Cuvettes are molded1

Cuvette5

Post electroporation3

Nanoknife1

Hepatocellular Carcinoma1

Electropermeabilization3

Transfection System3

20201

Cells37

Plasmid5

Eukaryotic1

Mesotherapy2

Dermal8

Efficiency1

Ablation1

Electrode1

Electroporate1

20191

Ligation1

Capillary1

Reversible1

Induces2

Protocols1

Treatment3

Embryos3

Membrane5

Electrofusion1

Deeply into the skin2

Microbiology1

Tissues1

Pulse1

Device2

Painless2

Scalable1

Pores2

Injection1

Treatments1

High6

Chamber2

Mechanisms1

System3

Efficient1

Cas91

Molecular1

Trends1

Suitable1

Figure3

Application1