Electrophoretic Mobility Shift AssayPromoter Regions, GeneticBase SequenceDNA-Binding ProteinsMolecular Sequence DataTranscription FactorsGA-Binding Protein Transcription FactorTranscription, GeneticBinding SitesSp1 Transcription FactorProtein BindingNF-kappa BDNA FootprintingGene Expression RegulationDNATranscriptional ActivationResponse ElementsGenes, ReporterTransfectionNuclear ProteinsRNA, MessengerTranscription Factor AP-1Cell LineLuciferasesTrans-ActivatorsSp3 Transcription FactorCell NucleusRegulatory Sequences, Nucleic AcidElectrophoresisEnhancer Elements, GeneticConsensus SequenceCells, CulturedRepressor ProteinsGene Expression Regulation, EnzymologicTumor Cells, Cultured5' Flanking RegionDNA PrimersProto-Oncogene Proteins c-etsGene Expression Regulation, BacterialOligodeoxyribonucleotidesMutationChloramphenicol O-AcetyltransferaseCCAAT-Enhancer-Binding ProteinsMutagenesis, Site-DirectedHeLa CellsUpstream Stimulatory FactorsDeoxyribonuclease IChromatin ImmunoprecipitationAmino Acid SequencePlasmidsBlotting, WesternBacterial ProteinsI-kappa B ProteinsNF-kappa B p50 SubunitOligonucleotidesCCAAT-Binding FactorElectrophoresis, Polyacrylamide GelRecombinant Fusion ProteinsSignal TransductionTranscription Factor RelAReverse Transcriptase Polymerase Chain ReactionSequence DeletionCloning, MolecularProto-Oncogene Proteins c-junOctamer Transcription Factor-1Homeodomain ProteinsCyclic AMP Response Element-Binding ProteinCell Line, TumorUp-RegulationTranscription Factor AP-2Recombinant ProteinsProto-Oncogene Protein c-ets-1Binding, CompetitiveSequence Homology, Nucleic AcidTranscription Initiation SiteEarly Growth Response Protein 1Gene ExpressionHost Cell Factor C1PhosphorylationThiocarbamatesDimerizationProto-Oncogene ProteinsOligonucleotide ProbesTumor Necrosis Factor-alphaDown-RegulationBlotting, NorthernZinc FingersBlotting, SouthwesternArtificial Gene Fusion3T3 CellsNFI Transcription FactorsGene DeletionYY1 Transcription FactorE-Box ElementsReceptors, Cytoplasmic and NuclearErythroid-Specific DNA-Binding FactorsGene Expression Regulation, ViralImmediate-Early ProteinsHigh Mobility Group ProteinsTATA Box