• Equilibrium binding isotherms were obtained by plotting the signal intensity (nm shift) against analyte concentration (μM). The KD values were determined by fitting the curve using non-linear regression. (moleculardevices.com)
  • The live-cell identification of distinct cell populations has most commonly been accomplished with gene expression assays that rely on the detection of fluorescent reporter proteins under the transcriptional control of the gene of interest. (elifesciences.org)
  • In this study, computer analysis of the gonococcal genome sequence database, lacZ reporter fusions, and gel mobility shift assays were used to elucidate the regulatory mechanism by which expression of the farAB operon is modulated by MtrR in gonococci. (asm.org)