• An electrophoretic mobility shift assay (EMSA) or mobility shift electrophoresis, also referred as a gel shift assay, gel mobility shift assay, band shift assay, or gel retardation assay, is a common affinity electrophoresis technique used to study protein-DNA or protein-RNA interactions. (wikipedia.org)
  • Gel shift assays or electrophoretic mobility shift assay (EMSA) protocols usually require labelling DNA by radioisotope, digoxygenin, or biotin. (selectscience.net)
  • EMSA and dual-luciferase reporter assays further confirmed that STM3 could directly bind the promoter region to activate FUL1 expression. (nature.com)
  • The relationship between FENDRR and dynamin-related protein 1 (DRP1) was explored using bioinformatics analysis, Chromatin Isolation by RNA Purification (CHIRP), Electrophoretic mobility shift assay (EMSA) and Methylation-Specific PCR (MSP) assays. (biomedcentral.com)
  • Common target genes, with a role in modulating bone-cell function, were confirmed using a combination of siRNA, quantitative reverse transcriptase PCR (qRT-PCR), ELISA, promoter reporter analysis, Electrophoretic Mobility Shift Assay (EMSA) and chromatin immunoprecipitation (ChIP) assays. (biomedcentral.com)
  • The interactions between AmtR and the promoter regions of the three operons were confirmed by electrophoretic mobility shift assays (EMSAs). (frontiersin.org)
  • Purified AgrA showed high-affinity binding to the RNAIII-agr intergenic region by electrophoretic mobility shift assays (EMSAs). (usda.gov)
  • Electrophoretic mobility shift assays (EMSAs) and Western blot analyses were used to monitor both the DNA binding and expression of the transcription factors required to ensure basal transcription of the α5 gene. (nih.gov)
  • Typically one compound is labeled to follow its mobility during electrophoresis. (uams.edu)
  • Electrophoretic gel shift analysis was performed in sodium dodecyl sulphate-polyacrylamide gel electrophoresis with biotinylated EDIII antigen in the presence and absence of streptavidin. (cdc.gov)
  • In functional assays, DR8 and IR0 elements act as independent RAREs, whereas DR0 does not. (nih.gov)
  • Objective: The aim of this study was to identify DA-associated functional genetic variants through next-generation sequencing (NGS), bioinformatics, and functional assays. (cdc.gov)
  • Full method validation was performed on both the ATI- and IFX-HMSA, and the clinical sample test results were also compared with those obtained from a bridging ELISA method to evaluate the difference in performance between the two assays. (nih.gov)
  • ZIKV Biotinylated-EDIII antigen capture ELISA in study of novel assay to measure ZIKV seroprevalence in the Philippines. (cdc.gov)
  • The method provides substantial improvements, including minimal matrix effects and high specificity when compared with previously used oligonucleotide ƒ u detection methods such as ligand binding assays or liquid scintillation. (aspetjournals.org)
  • The hybridization LC-MS/MS platform provided unique advantages, including minimal matrix effects and high specificity, compared with traditional ligand binding assays or liquid scintillation approaches, which enabled efficient and reliable in vitro protein binding assay. (aspetjournals.org)
  • To examine the mechanism underlying DES-mediated regulation of Fas and FasL, we performed luciferase assays using T cells transfected with luciferase reporter constructs containing full-length Fas or FasL promoters. (aspetjournals.org)
  • DNA constructs were cloned into a pGL3 promoter vector for luciferase gene reporter assays. (cdc.gov)
  • Binding was localized by DNase I protection assays to a pair of direct repeats in the P2 and P3 promoter regions of the agr locus. (usda.gov)
  • Loss of function was confirmed by transcriptional activity assays, quantitative real-time PCR, and electrophoretic mobility shift assays. (ijbs.com)
  • RT-PCR assays revealed that the transcriptional levels of the rim genes were consistent with the changes in rimocidin production in the recombinant strains. (biomedcentral.com)
  • Electrophoretic mobility shift assays were performed in order to examine nuclear factor (NF)-κB and activator protein (AP)-1 binding activities. (ersjournals.com)
  • There was shift in mobility of probes (ERE or NF-κB2) of both Fas and FasL in the presence of nuclear proteins from DES-treated cells, and the shift was specific to DES because these probes failed to shift their mobility in the presence of nuclear proteins from vehicle-treated cells. (aspetjournals.org)
  • Risk and nonrisk oligonucleotides were tested for binding of nuclear extracts from A549, BEAS-2B, and IMR-90 lung cell lines by using electrophoretic mobility shift assays. (cdc.gov)
  • Electrophoretic mobility shift assays detected allele-dependent nuclear protein binding in A549 cells for 8 of 21 variants. (cdc.gov)
  • Western blot, Lactate dehydrogenase (LDH) release assay, Annexin V-FITC/PI double staining, Hoechst 33342/PI fluorescence staining and Caspase-1 activity assay were used to detect the role of FENDRR in HPAEC pyroptosis. (biomedcentral.com)
  • Plasma lipoproteins are macromolecular complexes of lipids and proteins that are classified by density and electrophoretic mobility. (medscape.com)
  • Viability and radiation response of macrophages after exposure to tea extracts was measured by MTT assays. (biomedcentral.com)
  • Consensus sequence oligonucleotides for the transcription factor of interest will be able to compete for the binding, eliminating the shifted band, and must be confirmed by supershift. (wikipedia.org)
  • Gel shift assays are often performed in vitro concurrently with DNase footprinting, primer extension, and promoter-probe experiments when studying transcription initiation, DNA gang replication, DNA repair or RNA processing and maturation, as well as pre-mRNA splicing. (wikipedia.org)
  • Variants of the competition assay are useful for measuring the specificity of binding and for measurement of association and dissociation kinetics. (wikipedia.org)
  • A mobility shift assay is electrophoretic separation of a protein-DNA or protein-RNA mixture on a polyacrylamide or agarose gel for a short period (about 1.5-2 hr for a 15- to 20-cm gel). (wikipedia.org)
  • However, assuming that the protein is capable of binding to the fragment, the lane with a protein that binds present will contain another band that represents the larger, less mobile complex of nucleic acid probe bound to protein which is 'shifted' up on the gel (since it has moved more slowly). (wikipedia.org)
  • An antibody that recognizes the protein can be added to this mixture to create an even larger complex with a greater shift. (wikipedia.org)
  • This method is referred to as a supershift assay, and is used to unambiguously identify a protein present in the protein - nucleic acid complex. (wikipedia.org)
  • To overcome these limitations, we have developed a non-radiolabeled homogeneous mobility shift assay (HMSA) to measure the antibodies-to-infliximab (ATI) and IFX levels in serum samples. (nih.gov)
  • An electrophoretic technique for assaying the binding of one compound to another. (uams.edu)
  • Although precursors can be found in earlier literature, most current assays are based on methods described by Garner and Revzin and Fried and Crothers. (wikipedia.org)
  • Dysregulation of the NF-κB pathway in B cells from PPARγ +/- was indicated by spontaneous NF-κB activation, as well as increased IκBα phosphorylation and gel-shift activity following LPS stimulation. (jci.org)
  • If the labeled compound is bound by the other compound, then the mobility of the labeled compound through the electrophoretic medium will be retarded. (uams.edu)
  • Using a two-color assay to simultaneously monitor poly(A) tail removal from different RNAs, we demonstrate that Puf3 can distinguish between RNAs of very similar sequence. (elifesciences.org)
  • The rimR 2 deletion and complementation assays were conducted to explore its role. (biomedcentral.com)