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  • tissues
  • In the case of solid tissues or cells, these are often first broken down mechanically using a blender (for larger sample volumes), using a homogenizer (smaller volumes), by sonicator or by using cycling of high pressure, and a combination of biochemical and mechanical techniques - including various types of filtration and centrifugation - may be used to separate different cell compartments and organelles prior to electrophoresis. (wikipedia.org)
  • mobility
  • Glycine has very low net charge at pH 6.8 of stacking gel, so it has low mobility. (wikipedia.org)
  • In this way, the difference in mobility of the polypeptide chains in the gel can be attributed solely to their size as opposed to both their size and charge. (wikipedia.org)
  • Chemical
  • Note: Maxam-Gilbert chemical DNA sequencing can be run alongside the samples on the polyacrylamide gel to allow the prediction of the exact location of ligand binding site. (wikipedia.org)
  • shift
  • Gel shift assays are often performed in vitro concurrently with DNase footprinting, primer extension, and promoter-probe experiments when studying transcription initiation, DNA replication, DNA repair or RNA processing and maturation, as well as pre-mRNA splicing. (wikipedia.org)
  • polymers
  • In addition, a gel filtration experiment was performed to see if the underestimation was caused by the presence of albumin polymers in the purified and heat-treated product, which might interfere with sample diffusion in the Vitros slide. (thefreelibrary.com)
  • filtration
  • 254,255 Stereoacuity declines precipitously as a function of the distance of the test objects from the convergence plane (Fig. Determine precise radioligand concentration as in gel filtration protocol above. (imbc-med.ru)
  • thus
  • Later, Daniel Nathans and Kathleen Danna showed that cleavage of simian virus 40 (SV40) DNA by restriction enzymes yields specific fragments that can be separated using polyacrylamide gel electrophoresis, thus showing that restriction enzymes can also be used for mapping DNA. (wikipedia.org)
  • water
  • After repeated washing with water, the gel is incubated in a silver nitrate solution. (wikipedia.org)