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  • separation
  • It allows separation of hundreds to thousands of proteins in one gel. (biomedcentral.com)
  • When an electric field is applied, the DNA will begin to move through the gel, at a speed roughly inversely proportional to the length of the DNA molecule (shorter lengths of DNA travel faster) - this is the basis for size dependent separation in standard electrophoresis. (wikipedia.org)
  • molecular
  • The gel therefore acts like a molecular sieve when the current is applied, separating the proteins on the basis of their molecular weight with larger proteins being retained higher in the gel and smaller proteins being able to pass through the sieve and reach lower regions of the gel. (wikipedia.org)
  • Novex┬« Tricine Gels are ideal for peptides and low molecular weight proteins (less than 10 kDa). (thermofisher.com)
  • A gel is a molecular mesh, with holes roughly the same size as the diameter of the DNA string. (wikipedia.org)
  • novel
  • Considering that our strategy allows highly parallel protein quantitation on 2-DE gels, it holds promise to accelerate the discovery of novel serum protein biomarkers. (jcvi.org)
  • Analysis
  • All gel images were scanned and digitized and then compared using Z3 Image Analysis Software (Compugen). (arvojournals.org)
  • The 5 images from 5 replicate 2-D analysis of each plasma sample were used to compile a master reference gel composite. (arvojournals.org)
  • different
  • Since the proteins from the different sample types (e.g. healthy/diseased, virulent/non-virulent) are run on the same gel they can be directly compared. (wikipedia.org)
  • buffers
  • 10 kDa) is hindered by the continuous accumulation of free dodecylsulfate (DS) ions (from the SDS sample and running buffers) in the stacking gel. (thermofisher.com)
  • silver
  • In the former case, a silver colloid is applied to the gel. (wikipedia.org)
  • The amount of silver can be related to the darkness, and therefore the amount of protein at a given location on the gel. (wikipedia.org)
  • The left image shows a silver-stained gel of embryo chick retina at ED7 and the right image is that of embryo chick retina at ED11. (nih.gov)
  • sample
  • Load the sample immediately on the gel. (thermofisher.com)
  • After the gel electrophoresis, the gel is scanned with the excitation wavelength of each dye one after the other, so we are able to see each sample separately (if we scan the gel at the excitation wavelength of the Cy3 dye, we will see in the gel only the sample that was labeled with that dye). (wikipedia.org)
  • Techniques
  • The chief advantage of two-dimensional techniques is that they offer a large increase in peak capacity, without requiring extremely efficient separations in either column. (wikipedia.org)
  • method
  • A method which utilizes computerized microdensitometry to measure amino acid compositions of polypeptides resolved in two-dimensional gels is described. (wiley.com)
  • Since the amounts of each protein in the internal standard is known to be the same in every gel, this method reduces inter-gel variation.electrophoresis incorporating a pooled internal standard. (wikipedia.org)