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  • agaroses
  • A variety of chemically modified agaroses with different melting and gelling temperatures are available through chemical modifications. (wikipedia.org)
  • The melting and gelling temperatures of agarose can be modified by chemical modifications, most commonly by hydroxyethylation, which reduces the number of intrastrand hydrogen bonds, resulting in lower melting and setting temperatures than standard agaroses. (wikipedia.org)
  • Gelidium
  • The gelling agent in agar is an unbranched polysaccharide obtained from the cell walls of some species of red algae, primarily from the genera Gelidium and Gracilaria. (wikipedia.org)
  • Agar was first subjected to chemical analysis in 1859 by the French chemist Anselme Payen, who had obtained agar from the marine algae Gelidium corneum. (wikipedia.org)
  • mixture
  • The remnant lipoproteins were isolated from postprandial plasma in hyperlipidemic subjects using the immunoaffinity gel mixture of anti apo A-1 and anti apo B-100 monoclonal antibodies and ultracentrifugation. (biomedsearch.com)
  • In this assay, a naturally competent tryptophan auxotrophic mutant of Acinetobacter baylyi (BD4 trpE27) is transformed with the total DNA of a putative Acinetobacter isolate and the transformation mixture is plated on a brain heart infusion agar. (wikipedia.org)
  • procedure
  • However, when charges are not all uniform then, the electrical field generated by the electrophoresis procedure will affect the species that have different charges and therefore will attract the species according to their charges being the opposite. (wikipedia.org)
  • exhibits
  • Agarose exhibits the phenomenon of thermal hysteresis in its liquid-to-gel transition, i.e. it gels and melts at different temperatures. (wikipedia.org)
  • Agar exhibits hysteresis, melting at 85 °C (358 K, 185 °F) and solidifying from 32-40 °C (305-313 K, 90-104 °F). This property lends a suitable balance between easy melting and good gel stability at relatively high temperatures. (wikipedia.org)
  • suitable
  • Agarose gel is easy to cast, has relatively fewer charged groups, and is particularly suitable for separating DNA of size range most often encountered in laboratories, which accounts for the popularity of its use. (wikipedia.org)
  • biomolecules
  • The removal of agaropeptin in agarose substantially reduce the EEO, as well as reducing the non-specific adsorption of biomolecules to the gel matrix. (wikipedia.org)
  • species
  • Prior to that time the Allegheny Mountain dusky salamander (Desmognathus ochrophaeus) had been thought to be a single species but studies using nucleic acid sequencing and agar gel electrophoresis have since shown it to be a species complex. (wikipedia.org)
  • Agar forms the supporting structure in the cell walls of certain species of algae, and which is released on boiling. (wikipedia.org)
  • analysis
  • Ten FFPE samples from 6 cases of amyotrophic lateral sclerosis (ALS) and 4 neurologically normal controls were selected for miRNA analysis on the basis of the following criteria for RNA quality: (i) a postmortem interval of less than 6 hours, (ii) a formalin fixation time of less than 4 weeks, (iii) an RNA yield per sample of more than 500 ng, and (iv) sufficient quality of the RNA agarose gel image. (nih.gov)
  • Although sharing many characterization criteria, L. jensenii differed from the similar Lactobacillus leichmannii in a gel electrophoresis analysis of their respective lactic dehydrogenases. (wikipedia.org)
  • samples
  • If several samples have been loaded into adjacent wells in the gel, they will run parallel in individual lanes. (wikipedia.org)
  • media
  • Agar has been used as an ingredient in desserts throughout Asia, and also as a solid substrate to contain culture media for microbiological work. (wikipedia.org)
  • However
  • However, with the outbreak of World War II many nations were forced to establish domestic agar industries in order to continue microbiological research. (wikipedia.org)
  • Low-concentration gels (0.1 - 0.2%) however are fragile and therefore hard to handle. (wikipedia.org)
  • growth
  • The growth is then harvested after incubation for 24 h at 30 °C, plating on an Acinetobacter minimal agar (AMA), and incubating at 30 °C for 108 h. (wikipedia.org)
  • grown
  • In medical literature, the bacterium is often referred to as S. aureus, Staph aureus or Staph A.. S. aureus appears as staphylococci (grape-like clusters) when viewed through a microscope, and has large, round, golden-yellow colonies, often with hemolysis, when grown on blood agar plates. (wikipedia.org)
  • consists
  • The electric field consists of a negative charge at one end which pushes the molecules through the gel, and a positive charge at the other end that pulls the molecules through the gel. (wikipedia.org)
  • solid
  • Commonly used media include liquids such as Middlebrook 7H9 or 7H12, egg-based solid media such as Lowenstein-Jensen, and solid agar-based such as Middlebrook 7H11 or 7H10. (wikipedia.org)
  • Common
  • Common artifacts in silver stained gels are bands of keratin in the ranges of 54-57 kDa and 65-68 kDa as a contamination of the sample prior to the electrophoresis. (wikipedia.org)
  • Genomic restriction fragment analysis of diverse host and geographical panels of S. iniae isolates has shown common profiles between virulent fish and human strains, though multiple pulsed field gel electrophoresis patterns have been identified among human isolates. (wikipedia.org)
  • Over the following centuries, agar became a common gelling agent in several Southeast Asian cuisines. (wikipedia.org)
  • adjacent
  • If several samples have been loaded into adjacent wells in the gel, they will run parallel in individual lanes. (wikipedia.org)
  • technique
  • DNA Gel electrophoresis is usually performed for analytical purposes, often after amplification of DNA via polymerase chain reaction (PCR), but may be used as a preparative technique prior to use of other methods such as mass spectrometry, RFLP, PCR, cloning, DNA sequencing, or Southern blotting for further characterization. (wikipedia.org)
  • water
  • After repeated washing with water, the gel is incubated in a silver nitrate solution. (wikipedia.org)