• We used x-ray crystallography and cryo-electron microscopy to define two distinct binding epitopes. (rcsb.org)
  • Scientists used x-ray crystallography and Cryo-electron microscopy to obtain the protein's structure. (isaaa.org)
  • To do so, we use X-ray crystallography, electron cryo-microscopy (cryo-EM) together with biochemical techniques to characterize the molecular mechanism of GPCR signalling complexes. (psi.ch)
  • Image A. is at near atomic resolution, secured via x-ray crystallography and cryo-electron microscopy. (cshl.edu)
  • Stillman's collaborator, CSHL Professor and HHMI Investigator Leemor Joshua-Tor visualized the human ORC complex using X-ray crystallography and cryo-electron microscopy (cryo-EM). (cshl.edu)
  • You will find out about the experimental techniques, such as X-ray crystallography, nuclear magnetic resonance and electron microscopy, which are used to determine the 3D structure of proteins. (manchester.ac.uk)
  • Outline methods for 3D structure determination by X-ray crystallography, NMR and electron microscopy. (manchester.ac.uk)
  • Electron Crystallography research line develops new transmission electron microscopy techniques for the investigation of inorganic, organic and biological materials. (iit.it)
  • Our TEM (Trasmission Electron Microscopy) has been modified in order to collect 3D electron diffraction data (electron diffraction tomography) EDT on any kind of nanocrystalline material. (iit.it)
  • The TSRI researchers then evaluated the new Env trimer using advanced versions of two imaging methods, X-ray crystallography and electron microscopy. (scripps.edu)
  • The Department of Bionanoscience will provide outstanding infrastructure for nanofabrication, fluorescence microscopy molecular/cell biology, biochemistry, state-of-the-art electron microscopy and high-performance computing for image processing. (academictransfer.com)
  • Previous experience with electron microscopy or X-ray crystallography is beneficial, but not required. (academictransfer.com)
  • Some of them can be seen experimentally, for example with X-ray crystallography or cryo-electron microscopy. (biorxiv.org)
  • These include X-ray crystallography, cryo-electron microscopy (cryo-EM), nuclear magnetic resonance (NMR), small-angle scattering and many others ( 1 ). (biorxiv.org)
  • Difference imaging of adenovirus: bridging the resolution gap between X-ray crystallography and electron microscopy. (microbiologyresearch.org)
  • To address these challenges, we use X-ray crystallography and cryo-electron microscopy (cryo-EM) in combination with biophysical, biochemical, and cell biological methods. (psi.ch)
  • Here we report the cryo-electron microscopy structure of a tubular HIV-1 capsid-protein assembly at 8 Å resolution and the three-dimensional structure of a native HIV-1 core by cryo-electron tomography. (nature.com)
  • The cryo-electron-microscopy structures enable modelling by large-scale molecular dynamics simulation, resulting in all-atom models for the hexamer-of-hexamer and pentamer-of-hexamer elements as well as for the entire capsid. (nature.com)
  • To do so, they work closely with instrumentation developers and colleagues across the European Photon and Neutron (EPN) science campus to obtain the best possible data from synchrotron X-ray diffraction or cryo-electron microscopy experiments. (embl.org)
  • The work typically involves structure determination by X-ray crystallography or cryo-electron microscopy and associated functional studies. (embl.org)
  • The Cusack group uses X-ray crystallography and cryo electron-microscopy (cryoEM) to study the structural biology of protein-RNA complexes involved in RNA virus replication, innate immunity and cellular RNA metabolism. (embl.org)
  • Theoritical and Hands-on course in biomolecular integrative structural Biology by SV specialists in the field of X-ray crystallography, Electron Microscopy, Bio-NMR and Mass Spectrometry. (epfl.ch)
  • He also performs unique dermatologic research studying the three-dimensional structures of skin-related proteins using x-ray crystallography and cryo-electron microscopy. (yale.edu)
  • He performs laboratory research with the goal of using x-ray crystallography, cryo-electron microscopy, and biochemistry to better understand the structure-function relationship of proteins involved in normal and diseased skin and dermatologic therapeutics. (yale.edu)
  • In the study, they determined the optimum time required to produce the most uniform crystals of the CZTS semiconductor - 18 minutes in the microwave oven - and confirmed the material indeed was CZTS by using a variety of tests, such as X-ray crystallography, electron microscopy, atomic force microscopy and ultraviolet spectroscopy. (scienceblog.com)
  • These processes are controlled by the assembly of signalling complexes that can only be visualised through X-ray crystallography and electron cryo-microscopy, and therefore Dr Aylett is also engaged in developing new techniques for sample preparation and analysis in these fields. (imperial.ac.uk)
  • The work in the Gouaux Lab is concentrated on developing molecular mechanisms for the function of receptors and transporters at chemical synapses by utilizing cryo-electron microscopy, x-ray crystallography, and electrophysiology. (ohsu.edu)
  • The research activities of the CSB revolve around three major biophysical approaches for studying the structure and dynamics of complex biological macromolecules: NMR spectroscopy, X-ray crystallography, and electron cryo-microscopy. (stonybrook.edu)
  • We employ a multidisciplinary approach including kinase biochemistry and biology, structural biology (X-ray crystallography and cryo-electron microscopy), assay development, high-throughput screening, proteomics, imaging and chemical biology to gain high-resolution insights into kinase/pseudokinase signaling proteins. (edu.au)
  • use of x-ray diffraction and electron microscopy in mineral analysis. (middlebury.edu)
  • The derivation of molecular models from spatial density data generated by X-ray crystallography or electron microscopy is an active field of research. (uzh.ch)
  • Although high-resolution structural information can be obtained by X-ray crystallography, cryo-electron microscopy, nuclear magnetic resonance, and structure prediction tools, they are often insufficient for grasping the actual states of proteins in solution. (biophysics.org)
  • By using an integrative approach which combines cryo-electron microscopy and X-ray crystallography, the Unit of Structural Dynamics of Macromolecules deciphered the recruitment and cooperativity mechanisms between DNA polymerase D and PCNA. (pasteur.fr)
  • The make-up and complexity of these molecular machines present perfect problems for structural biology and many of the protein components of these machines have been examined using X-ray crystallography or cryo-Electron Microscopy. (diamond.ac.uk)
  • Our research builds on hybrid use of state-of-the-art cryo electron microscopy, X-ray crystallography and advanced optical microscopy to elucidate blueprints of dynamic protein and principles of their assembly and cellular level interplay. (unibas.ch)
  • We have resolved the architecture of human mTORC1and mTORC2 (Fig. 1) by a combination of crystallographic studies and cryo electron microscopy in collaboration with Mike Hall (Biozentrum) and Nenad Ban (ETH Zurich). (unibas.ch)
  • Figure 1: The architectures of human mTORC1 and mTORC2 resolved by cryo electron microscopy (Aylett, Sauer & Imseng et al. (unibas.ch)
  • Through combining cryo-electron microscopy at different pHs, X-ray crystallography and biochemical and structural analysis, we showed that NendoU can shift between open and closed forms, which probably correspond to active and inactive states, respectively. (lu.se)
  • Surface plasmon resonance, x-ray crystallography, and cryo-electron microscopy determined binding kinetics and structural data. (bvsalud.org)
  • Cryo-electron microscopy reveals a planar and roughly symmetrical complex formed by REGN5713/14/15 bound to Bet v 1. (bvsalud.org)
  • Research focus on X-ray crystallography, small-angle X-ray scattering (SAXS) and electron microscopy. (lu.se)
  • Under electron microscopy (EM), amyloid deposits are composed of linear, nonbranching, aggregated fibrils that are 7.5-10 nm thick and of indefinite length and arranged in a loose meshwork. (medscape.com)
  • Infrastructures for transmission- and scanning electron microscopy, photoelectron spectroscopy, nuclear magnetic resonance and magnetic resonance imaging, mass spectroscopy, crystallography, additive manufacturing, as well as dedicated fabrication and synthesis laboratories for electronic and circular materials are being financed. (lu.se)
  • Lund University is funded 45,5 MSEK for electron microscopy, and the technology platform is run together with KTH and Stockholm University for a total of nearly 103 MSK. (lu.se)
  • At the Swiss Light Source macromolecular crystallography (MX) beamlines the collection of serial synchrotron crystallography (SSX) diffraction data is facilitated by the recent DA+ data acquisition and analysis software developments. (iucr.org)
  • The emergence of serial crystallography at the X-ray free-electron lasers (XFELs) and its subsequent adaptation at the synchrotron X-ray sources allowed macromolecular crystallography (MX) expansion towards previously inaccessible structural biology targets such as membrane proteins or large complexes, which tend to crystallize as micrometre-sized crystals. (iucr.org)
  • Macromolecular Crystallography (MX) at Diamond reveals the shape and arrangement of biological molecules at atomic resolution, knowledge of which provides a highly accurate insight into function. (diamond.ac.uk)
  • X-ray diffraction crystallography and infrared spectroscopy revealed that these fibrils have a meridional, antiparallel, beta-pleated sheet configuration with polypeptide chains arranged perpendicular to the long axis of the fibrils. (medscape.com)
  • We also investigated the conformational dynamics of the same protein by X-ray scattering, neutron scattering, and MD simulation. (biophysics.org)
  • For example, if a structure is solved by X-RAY DIFFRACTION AND NEUTRON DIFFRACTION, it will be counted only in this category. (sdsc.edu)
  • We focus on using scattering techniques to look at the structure and dynamics associated with these strong electron corrrelations, particularly neutron and X-ray methods. (lu.se)
  • To achieve this we use X-ray crystallography and single-particle cryoEM to determine structures after trapping successive states along the active transcription or replication pathways. (embl.org)
  • The X-ray structures of trimers of the protein and their complexes with various metals, single-particle electron microscopic structure of iron-free and iron-loaded 24-subunit oligomers. (lu.se)
  • Finally, thanks to technological breakthroughs at the European Synchrotron Research Facility in France, Nelson managed to collect single-crystal X-ray diffraction data from her tiny crystals. (acs.org)
  • Today, there is an evolving need for more imaging methods and for biophysics that cannot be addressed with crystallisation and crystallography. (infn.it)
  • Serial femtosecond crystallography is a promising new technique for protein structure determination, where a liquid stream containing protein crystals is intersected with a high-intensity X-ray free electron laser beam that is 109 times brighter than traditional synchrotron X-ray sources. (spectroscopyonline.com)
  • How did the combination of serial femtosecond crystallography and high-intensity X-ray free electron laser take place? (spectroscopyonline.com)
  • While the crystals diffract and immediately after are destroyed by the intense X-ray free electron lasers (XFELs) beam, the resulting diffraction patterns can be recorded with state-of-the-art detectors. (spectroscopyonline.com)
  • The technique is able to yield high-resolution structural information from small micro- or nanocrystals, thus reducing the contribution of crystal defects and avoiding tedious (if not impossible) growth of large crystals as is required in traditional synchrotron based crystallography. (spectroscopyonline.com)
  • Because the crystals were far too small to analyze by conventional single-crystal X-ray diffraction techniques, Nelson threw herself into determining their structure using powder X-ray diffraction of crystal aggregates. (acs.org)
  • The X-ray facility has the latest robots and imaging equipment for preparing crystals of macromolecules. (stonybrook.edu)
  • In 2013 we unveiled the method MicroED, electron diffraction of microscopic crystals, and demonstrated that it is feasible to determine high-resolution protein structures by electron crystallography of three-dimensional crystals in an electron cryo-microscope (CryoEM). (lu.se)
  • The crystals are often a billion times smaller in volume than what is normally used for other structural biology methods like x-ray crystallography. (lu.se)
  • Through the Joint Structural Biology Group (JSBG), EMBL staff collaborate with the ESRF in building and operating state-of-the-art X-ray beamlines, developing associated instrumentation and techniques, and providing expert help to visitors. (embl.org)
  • Researchers from the University of Sheffield have been using some of Diamond's crystallography beamlines to understand a particularly enigmatic piece of this tiny puzzle. (diamond.ac.uk)
  • The resolution attained by cryo-EM is very often lower than that of X-ray structures, mainly due to the structural heterogeneity of the measured samples. (biorxiv.org)
  • Dr Garau is author and co-author of more than fifty publications in high-quality journals, including JACS, PNAS, Nature Neuroscience and Structure (Cell), a book chapter (X-ray Structures and Mechanisms of Metallo-Beta-Lactamases), and received several research highlights with international collaborators. (iit.it)
  • X-ray and EM structures of magnesium chelatase. (lu.se)
  • Studies on iron homeostasis in mitochondria: X-ray structures of yeast frataxin, a mitochondrial protein involved in iron storage and detoxification, also known as a factor in the neurodegenerative disease Friedrich's Ataxia. (lu.se)
  • The use of molecular mechanics calculations to supplement experimental data in standard X-ray crystallography and NMR refinements is discussed and it is shown that structures can be locally improved by the use of quantum chemical calculations. (lu.se)
  • The most common strong alternative and complement to experimental methods to methods to obtain protein structures are X-ray crystallography obtain insight in biochemical processes. (lu.se)
  • This project will also provide the student with a unique opportunity to conduct high-resolution electron cryo-tomography in order to structurally characterise these complexes in their native cellular context. (academictransfer.com)
  • X-ray free electron lasers (XFELs) are pulsed and since the protein suspensions are typically injected in a continuous flow, sample injected in-between the XFEL's pulses is wasted. (spectroscopyonline.com)
  • He has been responsible for building the beamline FemtoMAX at MAX IV and is a frequent user of Synchrotron radiation facilities (including MAX IV) and Free-electron lasers. (lu.se)
  • LEAPS is the League of European Accelerator-based Photon Sources , a strategic consortium initiated by the Directors of the Synchrotron Radiation and Free Electron Laser (FEL) user facilities in Europe. (infn.it)
  • Powerful new data analysis methods have been developed, allowing a team to analyze these diffraction patterns and obtain electron density maps and detailed structural information of proteins. (spectroscopyonline.com)
  • The data collection procedure has been automatized and optimized in order to perform fast measurements even on materials sensitive to strong electron beams. (iit.it)
  • EMBL Grenoble has its own in house Glacios cryo-electron microscope for screening and data collection and participates with the other PSB institutes in running a Krios microscope installed at the ESRF for external users. (embl.org)
  • Other facilities include a Scanning Transmission Electron Microscope (STEM) in the Department of Biology. (stonybrook.edu)
  • metal site is extended X-ray absorption fine structure (EXAFS). (lu.se)
  • The external magnetic field induces currents of the electrons in molecular orbitals. (wikipedia.org)
  • The x-ray photons can also interact directly with the spin of the electrons, and so they can scatter off the magnetic structure. (lu.se)
  • High intensity XFELs are linear accelerators, creating an electron beam which is "wiggled" by undulators. (spectroscopyonline.com)
  • We are looking for a highly motivated PhD candidate to join the Biomolecular Electron Nanoscopy group of Dr. Arjen Jakobi at the Department of Bionanoscience, Kavli Institute of Nanoscience Delft ( http://cryoem.tudelft.nl ). (academictransfer.com)
  • Our field of investigation spans from mineralogy to solid state chemistry, from organic crystallography to protein structure determination. (iit.it)
  • In their work, the Unit of Structural Dynamics of Macromolecules presented the structure of the DNA-bound PolD-PCNA complex from P. abyssi at 3.77 Å using an integrative structural biology approach, combining cryo-EM, X-ray crystallography, protein-protein interaction measurements and activity assays. (pasteur.fr)
  • There are indeed other approaches to reducing sample waste, which all come at a price, such as either being too slow for very high XFEL repetition rates, not being compatible with time-resolved crystallography, not being compatible with many classes of proteins, or introducing large background. (spectroscopyonline.com)
  • Our method thus requires little to no adjustment compared to non-droplet approaches for the crystallographer and additionally can be adapted to time-resolved crystallography techniques, where sample requirements is a limiting factor. (spectroscopyonline.com)
  • If the incident photon energy is just right, it can excite a core atomic electron into the valence band. (lu.se)
  • The X-ray crystallography study was the first ever of an Env trimer, and both methods resolved the trimer structure to a finer level of detail than has been reported before. (scripps.edu)
  • Training will be provided in molecular biology, protein biochemistry, cryo-EM and associated image-processing techniques as well as X-ray crystallography. (academictransfer.com)
  • When the mechanism pairing the electrons together remains in debate, superconductors are classed as unconventional. (lu.se)
  • We developed a fast EDT procedure that, combined with a new generation single electron diffraction camera (MEDIPIX2 detector acquired thanks to a Regione Toscana contribution), allows fast data collection with dose lower that 0.05 e/(Å 2 s). (iit.it)
  • The new data are consistent with the findings on Env subunits over the last 15 years, but also have enabled us to explain many prior observations about HIV in structural terms for the first time," said Jean-Philippe Julien, a senior research associate in the Wilson laboratory at TSRI, who was first author of the X-ray crystallography study. (scripps.edu)
  • Fundamentals of Crystallography: the theory behind crystal structure solution. (iucr.org)
  • X-ray structure structure of human ornithine decarboxylase inhibitor complex. (lu.se)
  • X-rays interact with the electrons in matter, and are routinely used to establish the physical structure of materials, i.e. where the electrons in a given compound are spatially. (lu.se)
  • In our study, we exploited a paramagnetic lanthanide ion, Gd 3+ , in electron paramagnetic resonance (EPR) experiments and measured the inter-gadolinium distance distribution that reflects the conformational states of the protein. (biophysics.org)
  • The interactions of electrons in materials are a rich and complex source of physical problems, in part due to the issues brought about by dealing with the large number of electronic many-body interactions, both with other electrons and with the parent ions. (lu.se)
  • While X-ray crystallography captures single stable states, cryo-EM allows observing conformational ensembles in solution. (biorxiv.org)
  • They used I02, I03 and I24 in their study and plan to do follow up work using X-ray crystallography and Cryo-EM such as those at the eBIC centre at Diamond. (diamond.ac.uk)
  • Thanks to the strong interaction of the electrons with matter and the capability to focus electrons in nanometric bright beams, electron diffraction is the only single crystal diffraction technique that can be performed on nanocrystals. (iit.it)
  • Traditionally, X-ray crystallography was dominating the interaction of large facilities with life sciences. (infn.it)
  • Photosensitizers are used to obtain excited state electrons by harvesting sunlight and then transfer the excited electron to a semiconductor, substrate or another catalyst to drive solar cells and photocatalysis. (lu.se)
  • We have several projects ongoing in this area, including investigation of superconductivity in systems where the electrons are heavy, i.e. have a large effective mass, and explorations of the normal state from which superconductivity develops in the cuprate family of superconductors. (lu.se)