Electrodes immobilizing an enzyme. Medical search. Frequent questions

A glucose electrode consisting of a membrane of immobilized glucose oxidase is described. (justia.com)
When the enzyme electrode is placed in contact with a biological solution or tissue, the glucose and oxygen diffuse into the enzyme layer with a corresponding outward flow of hydrogen peroxide and gluconic acid. (justia.com)
Glucose oxidizing and oxygen reducing enzymes are immobilized on microelectrodes of an application specific integrated circuit (ASIC) using redox hydrogels to produce an enzymatic biofuel cell, capable of harvesting electrical power from just a single droplet of 5 mM glucose solution. (rsc.org)
The enzyme used for glucose detection, glucose oxidase, was attached to ZnO nanocombs which resulted in a biosensor that exhibits a high affinity, high sensitivity, and fast response for glucose detection. (nanowerk.com)
Laccase and glucose oxidase (GOx) were used as enzymatic biocatalysts, which were immobilized on the CNT walls and tips using an electrochemical technique. (njit.edu)
The amperometric responses of the laccase electrode upon additions of bubbled air and potentiometric responses of GOx electrode to additions of glucose demonstrated that the immobilized enzymes retained their bioelectrocatalytic activity after electrochemical deposition. (njit.edu)

Most lactate biosensors are made by immobilizing lactate oxidase (an enzyme) and an appropriate mediator on an electrode. (medicaldesignbriefs.com)
In referring to the new nanostructured materials applied in electrochemistry,large variety of devices have been developed for application in sensors and biosensors, fuel cells and catalysts, including those using biological molecules.For example, the new hybrid devices involving inorganic materials / organic and biomolecules, several specific substances may be studied as a function of molecular architecture used in the interface electrode / electrolyte. (fapesp.br)

The overall objective of this program is to develop an effective means for integrating enzymes onto an electrode in order to improve electron transfer and enzyme loading and stability. (sbir.gov)
Our objective is to significantly increase the electron transfer efficiency of the enzyme-catalyzed reactions at electrodes and thereby establish a new state-of-the-art power source for military and commercial systems. (sbir.gov)
Moreover, carbon nanotubes are known for their high chemical and electrochemical stability facilitating the electron transfer between the active site of enzymes and the electrode surface. (science24.com)
Comparing SERR and SEIRA data we propose that binding of Ca2+ to the heme induces protein reorientation in a way that the electron transfer pathway of the catalytic FAD center to the electrode can bypass the heme cofactor, resulting in catalytic activity at more negative potentials. (istanbul.edu.tr)
In Protein Film Voltammetry, a redox enzyme is immobilized on a rotating electrode in a configuration allowing fast, direct electron transfer. (hal.science)
The combination of p-Si/CNTs with redox enzymes provided a convenient prototype for a direct electron transfer, membrane-less biofuel cell. (njit.edu)

An L-Lactate Amperometric Enzyme Electrode Based on L-Lactate Oxidase Immobilized in a Laponite Gel on a Glassy Carbon Electrode. (univ-grenoble-alpes.fr)
Moreover, the common amperometric evaluation of AChE inhibition degree was correlated to the impedimetric changes of the electrode surface, showing a good correlation ( R 2 = 0.99 for carbamates and R 2 = 0.98 for organophosphates) between the two methods. (springer.com)
An amperometric biosensor based on acetylcholinesterase immobilized onto iron oxide nanoparticles/multi-walled carbon nanotubes modified gold electrode for measurement of organophosphorus insecticides. (springer.com)
The prepared working electrodes were characterized using scanning electron microscopy-energydispersive X-ray spectrometry, together with electrochemical impedance spectroscopic, cyclic voltammetric, and amperometric techniques. (ac.rs)

The approach is based on conformably coating a layer of functional polymer onto an arrayed multi-walled carbon nanotube (MWCNT) electrode. (sbir.gov)
The work presented in this thesis has focused on designing and characterizing biofuel cell electrodes using porous silicon (p-Si) as the substrate or current collecting platform on which carbon nanotubes (CNTs), both single-walled carbon nanotubes (SWNTs) and multi-walled carbon nanotubes (MiWNTs), were synthesized directly, followed by enzyme catalyst immobilization on the CNTs. (njit.edu)

We present here an enzymatic microbiofuel cell whose electrodes are directly integrated into a digital electronic circuit. (rsc.org)
Application of this enzymatic electrode was investigated in the cathode chamber of a MFC. (liu.se)
Limitation by mass transport of the substrate towards the electrode may obscure important features and complicate the analysis of the enzymatic response, so much so that the enzyme has high activity. (hal.science)

A biosensor based on the immobilization of Lactate oxidase in laponite-organosilasesquioxane films on glassy carbon electrode for the quantification of L-lactate in wine and dairy products is presented. (univ-grenoble-alpes.fr)
Detection of carbamic and organophosphorus pesticides in water samples using cholinesterase biosensor based on Prussian Blue modified screen printed electrode. (springer.com)
Acetylcholinesterase biosensor based on self-assembled monolayer-modified gold-screen printed electrodes for organophosphorus insecticide detection. (springer.com)

2] I. Švancara, K. Vytřas, K. Kalcher, A. Walcarius, and J. Wang, Carbon paste electrodes in facts, numbers, and notes: A review on the occasion of the 50-years jubilee of carbon paste in electrochemistry and electroanalysis. (edu.pl)
3] R. N. Adams, Carbon paste electrodes. (edu.pl)
5] R. N. Adams, Carbon paste electrodes: A Review. (edu.pl)
8] C. Olson and R. N. Adams, Carbon paste electrodes. (edu.pl)
Analytica Chimica Acta, 22 (1960): 582-589, plus C. Olson and R. N. Adams, Carbon paste electrodes application to cathodic reductions and anodic stripping voltammetry. (edu.pl)
9] T. Kuwana and W. G. French, Carbon paste electrodes containing some electroactive compounds. (edu.pl)
11] D.G. Davis and M.E. Everhart, Chronopotentiometry of the bromide-bromine couple at platinum and carbon paste electrodes. (edu.pl)
12] A. L. Beilby and B.R. Mather, Resistance effects of two types of carbon paste electrodes. (edu.pl)
Farsang, Voltammetric properties and analytical uses of carbon paste electrodes prepared with silicone oil. (edu.pl)
The analytical performance of carbon paste electrodes (CPE) bulk modified with ionic liquid 1-allyl-3-methylimidazolium dicyanamide ([AMIM][DCA]), and surface modified with platinum on graphitized carbon (Pt-C) or platinum-ruthenium alloy on graphitized carbon (Pt,Ru-C) for analysis of hydrogen peroxide (H 2O2) were compared. (ac.rs)

It was found that screen-printed gold electrodes and a very low pH value (pH 1) are well-suited to perform the electrochemical detection of TMB, due to the reversible character of the redox reaction under these conditions. (kobv.de)
In contrast to this, for carbon screen-printed electrodes, it was found that the signal response has changed after the electrochemical reaction with TMB at pH 1. (kobv.de)
At moderately acidic conditions (pH 4), neither with carbon nor with gold electrodes a reproducible electrochemical detection of TMB could be achieved. (kobv.de)
6] M. Březina, Estimation of electrochemical activity of carbon using a paste electrode. (edu.pl)

The various areas that Ludo Diels examines in his Membrane study include Immobilized enzyme, Process economics, Inorganic chemistry and Biochemical engineering. (research.com)

Based on their intrinsic properties, i.e., high specificity and high affinity for the substrate, enzymes may provide sustainable alternatives to currently used chemical catalysts for human health monitoring, biopower generation or high-value product synthesis. (mdpi.com)
we ivestigated how microbial fuel cell (MFC) performance can be affected by laccase and manganese peroxidase (MnP) enzymes as catalysts in the cathode compartment. (liu.se)
Enzymes are biological catalysts responsible for supporting almost all of the chemical reactions that maintain animal homeostasis . (golifescience.com)
In these anomalies, several enzymes are released into the blood, and quantification of these catalysts can lead to disease detection. (golifescience.com)

The nanobiosensor was constructed by immobilizing the enzyme horseradish peroxidase (HRP) electrostatically onto the surface of a rotating gold disk electrode modified with PDMA-PSS nanoparticles. (iupac.org)
One of the most popular strategies is enzymefunctionalized nanoparticles used as tracer to enhance the detection sensitivity by loading a large amount of enzymes toward a sandwich immunological reaction event. (cdc.gov)
In our group, various nanomaterils have been used as carrier to load enzymes and antibodies including graphene, carbon nanotubes (CNTs), carbon nanospheres, gold nanoparticles, silica nanoparticles and carboxylated magnetic beads. (cdc.gov)

However, enzyme bioelectrocatalysis suffers from low catalytic efficiency, imposing fundamental investigations on mechanisms of enzyme immobilization, including molecular basis knowledge of the efficient electronic communication between enzymes and the electrode. (mdpi.com)
It has been shown that the catalytic performance of this enzyme immobilized on electrodes can be increased by presence of calcium ions. (istanbul.edu.tr)
Spectroelectrochemical measurements of these systems will be systematically explored before and after laser application to assess the impact of photoactivation on increasing the catalytic activity of sensor and biosensor systems based on the enzyme redox alcohol dehydrogenase. (fapesp.br)
Enzymes as biocatalysts have been widely used in industrial processes such as food processing, beer fermentation, laundry detergents, pickling purposes, and control, as well as accelerate, the catalytic reactions in order to quickly and precisely obtain various valuable end products. (golifescience.com)
The amount of guanine per silica NP was determined with chronocoulometry by measuring the charges from the guanine catalytic oxidation at the SPE, which was immobilized with the functionalized silica. (cdc.gov)

The other used anti-VCM antibodies immobilized on a gold surface via the SAM (SAM-Ab). (iitani.com)

The scientists employed a method called electron beam-induced graft polymerization, by which functional molecules were bonded to a carbon-based material that can spontaneously bind to the enzyme. (medicaldesignbriefs.com)
This simple method of fabricating ZnO based biosensor can be extended to immobilize other enzymes and other bioactive molecules on various 1D metal oxide nanostructures, and form versatile electrodes for biosensor studies. (nanowerk.com)
The enzyme molecules were considered to be entrapped in a cross-linked matrix consisting of the carboxylic acid on the electrode and the cationic polymer. (go.jp)
Concerned are all important aspects of the field, from fundamental investigations with carbon paste as the electrode material, via laboratory examination of the first electrode prototypes, basic and advanced studies of various electrode processes and other phenomena, up to practical applications to the determination of inorganic ions, complexes, and molecules. (edu.pl)
13] C. A. H. Chambers and J. K. Lee, Studies of the extraction of organic molecules into the carbon-paste electrode. (edu.pl)
Biological recognition molecules are co-immobilized with enzymes on electrode arrays to sensitively and quantitatively measure target analytes having biomedical relevance. (msu.edu)

Commercial laccase was immobilized by crosslinking on chitosan using glutaraldehyde. (liu.se)
Cyclic voltammetry showed well-defined redox peaks which indicated that the enzyme (GOx and laccase) were successfully immobilized on the CNTs. (njit.edu)

The measurement of the serum levels of numerous enzymes has been shown to be of diagnostic significance as Diagnostic Enzymes . (golifescience.com)

2. A characteristic of living cells in suspension (a dispersion of fine solid or liquid particles in a fluid) and biological compounds (proteins) in a solution to travel in an electric field to the positive or negative electrode, because of the charge on these substances. (wordinfo.info)
His lab has particular expertise in the use of proteins as nanomachines and in the production of high-value products utilizing enzymes and biological cells. (msu.edu)

Hydrogen peroxide (H 2 O 2 ) was used to measure activity of the enzyme before injection of the herbicides into the electrolyte solution. (iupac.org)

A chemical sensor that is highly selective due to a specific enzyme incorporated into its structure. (wordinfo.info)
In this study, we present an enzyme-based fluorometric electrospun fiber sensor (eFES) mesh as a gas-phase biosensor in dry form. (iitani.com)

The temperature range generally oscillates between 40 °C and 60 °C, a characteristic interval of the immobilized form, which usually guarantees better conditions because it is more stable than the free form. (researchgate.net)
C, a characteristic interval of the immobilized form, which usually guarantees better conditions because it is more stable than the free form. (researchgate.net)
The working principle of the AChE biosensor exploits the capability of carbamate and organophosphate pesticides to form a stable complex with the enzyme, which causes an impedimetric change. (springer.com)
A stable electrode response to TMB could be achieved at pH 1 on gold electrodes. (kobv.de)
Conventional bio-based sensors are normally prepared as wet-type probes to maintain proteins such as enzymes in a stable state, resulting in limitations in the commercialization of sensors, their operating environment, and performance. (iitani.com)
The enzyme electrode was stable for a long period of time and was used for over 60 measurements. (iupac.org)

This technique is used to probe the mechanism of enzymes by quantitatively interpreting the response in current as a function of the experimental conditions. (hal.science)

Enzyme immobilisation technology is an effective means to improve sugar ester production through the employment of biocatalysts. (researchgate.net)

A chemical reaction involving lactate oxidase, the mediator, and free lactate results in the generation of a measurable current between electrodes - current that is roughly proportional to the concentration of lactate. (medicaldesignbriefs.com)
Chemical engineering which connect with Palladium-hydrogen electrode. (research.com)

Bioelectrocatalysis is also limited by long-term stability requiring groundbreaking strategies for enzyme protection and bioelectrode survival. (mdpi.com)
Improves immobilized enzyme stability. (bionime.com)

If we immobilize Huc on an electrode, the electrons can enter an electrical circuit from the enzyme surface and generate current. (livescience.com)
The approach is to develop a novel method for orienting enzymes immobilized onto the electrode surface that results in facilitated charge transfer. (sbir.gov)
The sensing electrodes are fabricated on a PET surface, including three working electrodes, one reference electrode, and one counter electrode. (bvsalud.org)
One was prepared by immobilizing the peptide aptamer directly on (Direct-Apt) or via a self-assembled monolayer (SAM) on a gold surface (SAM-Apt). (iitani.com)
To get insight into the Ca2+-induced changes in the immobilized enzyme we employ surface-enhanced vibrational (SERR and SEIRA) spectroscopy together with electrochemistry. (istanbul.edu.tr)
Enhanced sensitivity was achieved by introducing the multi-bioconjugates of HRP-Ab2-CNS onto the electrode surface through "sandwich" immunoreactions. (cdc.gov)

Detection of organophosphorus insecticides with immobilized acetylcholinesterase-comparative study of two enzyme sensors. (springer.com)
On the basis of the dual signal amplification of graphene sheets and the multi-enzyme labeling strategy, the developed immunosensor showed a 7-fold increase in detection signal compared to the immunosensor without graphene modification and CNS labeling. (cdc.gov)

However, such biofuel cells are limited by the difficult electrical wiring of enzymes and their poor adhesion to the electrodes. (science24.com)
Moreover, results of complete biofuel cells based on these electrodes will be presented. (science24.com)
Working biofuel cells with p-Si/SWNTs and p-Si/MWNTs based electrodes with immobilized enzymes were studied at room temperature in a 0. (njit.edu)

In this work, we derive equations taking into account mass transport of substrate, for the steady-state current generated by an enzyme following Michaelis-Menten kinetics and immobilized onto a hydrodynamic (e.g. rotating) electrode. (hal.science)

This modified electrode with MnP was utilized as cathode. (liu.se)
This augmentation of MFC performance was due to a higher cathode electrode potential with H 2 O 2 rather than oxygen. (liu.se)

Because of their role in maintaining life processes, the assay and pharmacological regulation of enzymes have become key elements in clinical diagnosis and therapeutics. (golifescience.com)
Focus is on developing new processes and materials for implantable electrode arrays for register neural signals in the brain. (lu.se)

By cyclic voltammetry it was found that the TMB electrochemistry is strongly dependent on the pH and the electrode material. (kobv.de)
By cyclic voltammetry, it could be shown that TMB electrochemistry is strongly dependent on the pH and the electrode material. (kobv.de)
In this review (with 500 refs), both electrochemistry and electroanalysis with carbon paste-based electrodes, sensors, and detectors are of interest, when attention is focused on the research activities in the years of new millennium. (edu.pl)
1] P. Zuman, Electrolysis with a dropping mercury electrode: J. Heyrovský's contribution to electrochemistry. (edu.pl)
4] R. N. Adams, Electrochemistry at Solid Electrodes (New York: M. Dekker, 1969). (edu.pl)

Other enzymes and lipases from other microorganisms were also tested in the biocatalytic esterification of mono-and disaccharides. (researchgate.net)

In this patented device an enzyme reaction is coupled to an electrode process. (justia.com)

With this work plan is to contribute to understanding of new immobilized enzyme systems in the presence of nanocomposites.Furthermore, the project will allow the candidate improve their knowledge, when will contact with new laboratory techniques for the synthesis of nanostructures, and use techniques for characterization of biomaterials and nanostructured materials. (fapesp.br)

IC(50)=0.91 microM to hMAO-A). In addition, 3e showed mixed inhibition of hAChE and competitive inhibition of hMAO-B in the enzyme kinetic studies. (inrae.fr)

Adjustment of the pH and coenzyme quantity in the eFES mesh also affected enzyme activity. (iitani.com)
Serum elevations of ALT activity are rarely observed except in parenchymal liver disease, since ALT is a more liver-specific enzyme than asparate aminotransferase (AST). (cdc.gov)
1975. [Effect of methyl parathion or zineb administration on the activity of some hepatic enzymes in rats]. (cdc.gov)

Patented barrel plated gold electrodes. (bionime.com)
Enzymatically oxidized TMB is quantified after addition of H2SO4 by amperometry with screen-printed gold electrodes in a custom-made wall-jet flow cell. (kobv.de)

Scientists studying a cousin of the bacteria responsible for tuberculosis and leprosy have discovered an enzyme that converts hydrogen into electricity, and they think it could be used to create a new, clean source of energy literally from thin air. (livescience.com)

These attributes, alongside the microbe's ubiquity and ability to be easily grown, could make the enzyme an ideal candidate for a power source in organic batteries, the researchers say. (livescience.com)

The diagnosis of the serum level of certain enzymes has been used as an indicator of cellular damage that results in the release of intracellular components into the bloodstream. (golifescience.com)
This is because the presence of these enzymes in the serum indicates that tissue or cellular damage has occurred resulting in the release of intracellular components into the blood. (golifescience.com)

It provided much better performance than the biofuel cell with p-Si/MWNTs electrodes, which yielded a peak power of 1.23 nW at 5.6 mV. (njit.edu)

The enzymes are more frequently used in both laboratories and commercially for the purpose of stereospecific bioconversion, utilization of waste into useful end products for a better environment, for the production of raw materials and many more. (golifescience.com)

To investigate the chemistry behind M. smegmatis' shocking ability, the scientists first isolated the Huc enzyme responsible for the process using chromatography - a lab technique which enables scientists to separate the components of a mixture. (livescience.com)

Additionally, 4c(2) as an Nrf2 activator could promote the nuclear translocation of Nrf2 protein and induce the expressions of Nrf2-dependent enzymes HO-1, NQO1, and GPX4. (inrae.fr)

The bioelectrode showed a very high sensitivity (0.33±0.01) A cm-2 M-1 and a short time response (10 s) for less than 1 U of enzyme. (univ-grenoble-alpes.fr)

Each specific enzyme is immobilized on its corresponding working electrode. (bvsalud.org)

Anatomy7

Organisms3

Diseases2

Chemicals and Drugs18

Analytical, Diagnostic and Therapeutic Techniques and Equipment48

Psychiatry and Psychology4

Phenomena and Processes17

Disciplines and Occupations7

Technology, Industry, Agriculture8

Information Science1

Health Care4

Glucose6

Biosensors2

Electron transfer6

Amperometric4

Multi-walled carbon2

Enzymatic3

Biosensor based3

Carbon paste electrodes10

Electrochemical4

Membrane1

Catalysts4

Nanoparticles3

Catalytic5

Antibodies1

Molecules6

Laccase2

Measurement1

Biological2

Electrolyte1

Sensor2

Stable6

Quantitatively1

Biocatalysts1

Chemical2

Stability2

Surface6

Detection2

Biofuel cells3

Substrate1

Cathode2

Processes2

Electrochemistry5

Biocatalytic1

Reaction1

Synthesis1

Kinetic1

Activity3

Gold2

Bacteria1

Organic1

Serum2

Performance1

Commercially1

Chemistry1

Protein1

Sensitivity1

Specific1