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  • nitric oxide sy
  • Auch CJ, Saha RN, Sheikh FG, Liu X, Jacobs BL, Pahan K. Role of protein kinase R in double-stranded RNA-induced expression of nitric oxide synthase in human astroglia. (rush.edu)
  • IFN-gamma inhibition of MCMV growth was independent of known mechanisms involving IFN-alpha/beta, tumor necrosis factor alpha, inducible nitric oxide synthase, protein kinase RNA activated (PKR), RNaseL, and Mx1, and did not involve IFN-gamma-induced soluble mediators. (nih.gov)
  • PERK
  • Among the four eIF-2α kinases present in mammals, PERK is most highly expressed in the pancreas, suggesting that it may be responsible for the high eIF-2α[P] levels found therein. (asm.org)
  • Proteins
  • After 20 hpi, the cells were 35S pulse-labeled for 2 h, proteins separated on an SDS-PAGE, and late viral protein synthesis visualized either by autoradiography (Figure 5A) or western blot analysis detecting adenovirus capsid proteins (Figure 5B). (nih.gov)
  • phosphorylate
  • Although substitution of SUI2 genes mutated at these sites for the wild-type gene have no obvious effect on cell growth, one test that we have used appears to demonstrate that the inability to phosphorylate these sites has a physiological consequence on eIF-2 function in S. cerevisiae. (asm.org)
  • Molecular
  • Although it is well established that asbestos exposure is a major risk factor in the development of mesothelioma (2) , the molecular steps in carcinogenesis remain unknown. (aacrjournals.org)
  • recombinant
  • To be able to study the function of the VA RNAI-derived 5′- and 3′-mivaRNAs on virus growth, the GFP cassette in Ad-ΔVA-GFP was replaced with the wild-type VA RNAI gene or VA RNAI genes with 5′- and 3′-seed sequence mutations (Figure 2), generating recombinant viruses Ad-VAI wt, Ad-VAI 5′-mut and Ad-VAI 3′-mut, respectively. (nih.gov)
  • Gene
  • Mutations in this gene have been found in individuals suffering from autosomal recessive pulmonary venoocclusive-disease-2. (nih.gov)
  • The human c-fps/fes gene product expressed ectopically in rat fibroblasts is nontransforming and has restrained protein-tyrosine kinase activity. (asm.org)
  • amino
  • Mutational analysis of SUI2 coupled with biochemical analysis of eIF-2 alpha maps the sites to the carboxyl region of SUI2 that correspond to Ser residues at amino acid positions 292, 294, and 301 that compose consensus casein kinase II sequences. (asm.org)
  • Mice
  • UPase1-/- mice exhibit activation of the liver HRI-eIF-2α-ATF4 heme-deficiency stress response pathway. (nih.gov)
  • However, we found high phosphorylated eIF-2α (eIF-2α[P]) levels in nonstressed pancreata of mice. (asm.org)
  • stress
  • Evaluation of the effects of uridine on ER stress and regulation of eIF-2α. (nih.gov)
  • tumor
  • Expression profiling identified processes likely responsible for 18-fluoro-2-deoxy-glucose uptake and tumor localization by positron emission tomography, and a role for hypoxia-inducible factor-1 was suggested. (aacrjournals.org)
  • We propose that, in cancer cells and the tumor microenvironment, activation of diverse eIF2α kinases followed by IFNAR1 downregulation enables multiple cellular components of tumor tissue to evade the direct and indirect anti-tumorigenic effects of Type 1 IFN. (elsevier.com)
  • mRNA
  • The miRNA seed sequence (nucleotides: 2-8) is the key element mediating pairing between an miRNA and a target mRNA (12). (nih.gov)
  • As shown in Figure 4, the steady-state level of TIA-1 mRNA was reduced in cells infected with the VA RNAI wild-type virus at both 16 and 20 hour post-infection (hpi) (compare lane 1 with 2 and 7). (nih.gov)
  • activity
  • This result indicated that NF-κB activation by PKR does not require its kinase activity and that it is independent of the PKR-eIF2α relationship. (asm.org)
  • cells
  • 1 , 2 Bortezomib (Velcade, PS-341) is a novel therapeutic agent that has been shown to selectively induce apoptosis in malignant cells. (bloodjournal.org)